CN109717348A - A method of the fresh-keeping cordyceps sinensis of controlled atmospheric packing containing argon gas - Google Patents
A method of the fresh-keeping cordyceps sinensis of controlled atmospheric packing containing argon gas Download PDFInfo
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Abstract
The present invention provides a kind of method of fresh-keeping cordyceps sinensis of the controlled atmospheric packing containing argon gas; it is related to cordyceps sinensis technical field of preservation of fresh; the method of the fresh-keeping cordyceps sinensis of controlled atmospheric packing of the present invention containing argon gas; first fresh cordyceps sinensis is impregnated in fresh-keeping liquid; it is soaked in overlay film liquid again after taking out drying; show to form layer protecting film in cordyceps sinensis, cordyceps sinensis coated with film is subjected to controlled atmospheric packing with the mixed gas containing argon gas, is realized to the fresh-keeping of cordyceps sinensis.The present invention carries out controlled atmospheric packing using the mixed gas containing argon gas, the weight-loss ratio decline that cordyceps sinensis can be significantly inhibited, delays the growth of water activity and polyphenol oxidase activity is delayed to increase the decline for slowing down soluble protein content, relative to conventional preservation method, fresh keeping time at least can achieve 75d, keep better effect to the color, smell and taste of fresh cordyceps sinensis.
Description
Technical field
The present invention relates to cordyceps sinensis technical field of preservation of fresh more particularly to a kind of controlled atmospheric packing containing argon gas fresh-keeping worm summers in winter
The method of grass.
Background technique
Cordyceps sinensis is a kind of high-grade nourishing rare traditional Chinese medicine, nutriment and active constituent containing there are many, including nucleosides,
Polysaccharide, sterol, fatty acid, protein, polypeptide and volatile component etc. have antitumor, adjusting immune system, promotion body thin
Cellular damage reparation, free radical resisting, anti-oxidant, anti-aging, protection cardiac muscle and vascular cell improve cardiovascular function, protection liver
Dirty, protection kidney adjusts respiratory system, adjusts hematological system and adjusts the functions such as blood lipid.
Cordyceps sinensis on the market is dry cordyceps sinensis, a large amount of volatile components in cordyceps sinensis drying process mostly at present
It can evaporate, part nutrition can be lost, and active constituent can inactivate, while being taken mouthfeel and being greatly reduced, and fresh and alive cordyceps sinensis can be most
Its nutriment of the reservation of limits and active constituent, and keep its unique faint scent and crispy texture.In addition, pharmacology
Studies have shown that fresh cordyceps sinensis is in the pharmacological actions such as immunological regulation, antitumor, anti-oxidant compared with dry cordyceps sinensis
Aspect all has better effects.Therefore, the fresh-keeping of fresh cordyceps sinensis is urgently to be solved in the process of manufacture faced at present
Most significant problems.
Preservation method about fresh cordyceps sinensis is one of the hot spot direction of cordyceps sinensis research in recent years, studies main collection
In preservation by low temperature, quick-freezing fresh-keeping, in terms of, related patents method is as follows:
((CN 102406164A) discloses a kind of fresh and alive cordyceps sinensis processing and its can preparation side to Chinese patent application
Method fresh cordyceps sinensis will be mainly classified after cleaning in -70 DEG C of stored frozens of ultralow temperature, rear thawed by cordyceps sinensis,
It is immersed in the tap water fresh-keeping liquid containing citric acid, sodium isoascorbate, is examined, high temperature sterilization is prepared into can.
Chinese invention patent (CN 1037405928) discloses a kind of preservation method of cordyceps sinensis, to cordyceps sinensis
Head, after stroma and the cleaning of polypide branch, nutriment and active material are lost in order to prevent, by the fresh winter worm summer after cleaning
Grass, which is placed in 0.9% acid calcium lactate solution, impregnates 5-8min: quickly cooling handles 5~12h of cordyceps sinensis under the conditions of -75 DEG C again
Afterwards, it is placed in -5~-25 DEG C of vacuum cold case and saves.
Chinese invention patent (CN 1021782268) discloses a kind of fresh processing technique of cordyceps sinensis, mainly will
Fresh cordyceps sinensis deepfreeze under conditions of being lower than -65 DEG C；Mode cleaning and sterilization of the ultrasonic wave in conjunction with ozone is set simultaneously,
It is saved at last 0 DEG C.
Chinese patent application (105199956 A of CN) discloses a kind of preservation method of cordyceps sinensis, mainly uses one
After determining the cleaning of concentration alcoholic solution, being sprayed on cordyceps sinensis surface with hydromel saves nutrient water from damage, then carries out air-drying dehumidifying
Processing, then quick-frozen at 30 DEG C be placed on -5 DEG C of cold storage warehouses below and stored.
Chinese invention patent (106727734 A of CN) discloses a kind of fresh cordyceps sinensis product and preparation method thereof, mainly
It is by fresh cordyceps sinensis using flowing water and after being cleaned by ultrasonic, atomizing humidifying performance handles 10-40min, then uses 3-7%O2,75-
92%N2And 5-18%CO2Controlled atmospheric packing is carried out with fresh cordyceps sinensis is compared, the cordycepic acid content of fresh cordyceps sinensis product is not low
In 2%, polysaccharide is not less than 0.5%.
Chinese invention patent (106720232 A of CN) discloses a kind of storing and fresh-keeping method of fresh cordyceps sinensis, mainly
The methods of saved using Low-temperature cleaning, low temperature classification, classification, entire technique carries out at low ambient temperatures, makes fresh cordyceps sinensis
Fresh keeping time greatly prolongs, and reduces the loss of effective component, while reducing fresh-keeping cost.
Some fresh-keeping thinkings of the above prior art are saved in such a way that direct superfreeze is situated between 70 DEG C, Yi Xieshi
(0 DEG C or less) of low temperature preservation after the quick-frozen latching effective component of ultralow temperature (- 30 DEG C to -75 DEG C), also some technologies are sought to save from damage
It supports moisture or keeps color, increase the step of impregnating sour water or sprinkling hydromel, these prior arts provide fresh winter worm
Summer grass, compares dry cordyceps sinensis product and remains most nutriment and active constituent, but above-mentioned preservation method is big
Mostly using sense organ method from appearance color, smell, hardness, mouthfeel, flavour etc. variation aspect judgement sample freshness, it is subjective because
Element interference greatly, not can effectively solve loss of water in preservation process, soluble protein content decline, polyphenol oxidase enzyme activity still
Property increase etc. caused by cordyceps sinensis freshness decline the problem of.
The water content of the cordyceps sinensis of fresh acquisition is higher, and in storage period, its moisture content factor solid object surface steams
It rises and acts on and scatter and disappear, weightlessness can cause the symptoms such as sample softening physiology that its freshness is caused to decline.Soluble protein participates in
The regulation of a variety of Physiological and Biochemical Metabolism processes of edible mushroom is one of the important indicator of edible mushroom quality and nutrition.Under its content
Drop is considered as adopting an important feature of rear sample aging, with the extension of storage time, due to lacking in nitrogen source and mushroom body
The enhancing of prolease activity, accelerates the decomposition of protein, to change the flavor of mushroom body.Polyphenol oxidase is edible
The main enzyme of bacterium enzymatic browning can be such that product brown stain even ruins, the serious nutritive value for damaging edible mushroom, flavor and outer
Texture is seen, polyphenol oxidase activity is lower to illustrate that storage property can be better.
It is the novel preservation technology proposed in recent years that inert gas, which is used for fruit and vegetable air-conditioning preservation,.Hydrogen (Ar) belongs to lazy
Property gas, non-toxic and tasteless, price is lower.Under high pressure and certain temperature, hydrogen is dissolved into water, hydrone will form one kind
Polyhedron void structure is called " cage modle " compound.Cage compound can inhibit water activity, reduce the activity of enzyme, inhibit
Respiratory intensity, to achieve the purpose that inhibit fruits and vegetables brown stain.Rocculi etc. uses 90%Ar/5%O2/ 5%CO2Claim monkey to fresh-cut
Peach piece carries out controlled atmosphere, the results showed that argon gas can effectively inhibit respiratory rate.Wu etc. uses high pressure hydrogen combination low-temperature treatment
Fresh-cut Apples piece, the results showed that high pressure hydrogen can substantially reduce the reduction of total phenol content.DonglinZhang and
PeterC.Quantick etc. has studied influence of the Ar to tyrosinase and apple dehydrogenase activity, fills respectively into sample packaging
Enter air, N2, Ar, comparison discovery, Ar can be substantially reduced the activity of tyrosinase and apple dehydrogenase, and can protect for a long time
It holds.Guo Jia etc. research shows that argon gas controlled atmosphere to fresh berry it is fresh-keeping have positive effect, can by its freshness date expand 3 times with
On.Zhao Chunxia etc. is research shows that 90% argon gas processing mushroom can reduce certain respiratory intensity, and can effectively reduce solubility
The reduction of solid content.
But currently used controlled atmosphere technology is the concentration ratio for adjusting oxygen, carbon dioxide and nitrogen, such as China
Patent (CN 106923139A) discloses " a kind of intelligent fresh-keeping system of fresh cordyceps sinensis ", the mixed gas which uses
Proportion is 3-5%O2, 19-23%CO2, 74-76%N2；Chinese patent (CN 106923139A) discloses a kind of " fresh cordyceps sinensis
Product and preparation method thereof ", the controlled atmospheric packing gas mixing ratio which uses is 3-7%O2, 5-18%CO2, 75-92%N2.So far
Until the present, the report that controlled atmosphere is carried out using inert gas animal this to cordyceps sinensis-plant complex yet there are no.
Summary of the invention
Existing fresh cordyceps sinensis preservation method is short for cordyceps sinensis fresh keeping time, relies on equipment in order to overcome by the present invention
Property high defect, provide it is a kind of be suitable for fresh-keeping fresh cordyceps sinensis method, it is small to device dependence, it is easy to operate, for a long time
Fresh-keeping fresh cordyceps sinensis quality is good.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of methods of fresh-keeping cordyceps sinensis of the controlled atmospheric packing containing argon gas, comprising the following steps:
(1) fresh cordyceps sinensis is soaked in fresh-keeping liquid, takes out drying, the cordyceps sinensis after being impregnated；
The fresh-keeping liquid includes 1~3mmol/L D-sorbite, 1-3mmol/L vitamin C or ISOASCORBIC ACID, 6~9 μ
The L-cysteine concentration of mol/L phytic acid, 0.4~0.8mg/kg；
(2) cordyceps sinensis after immersion is soaked in overlay film liquid, takes out drying, obtains the cordyceps sinensis of overlay film；
The overlay film liquid includes the ethanol extract of 30~100mg/ml Cordyceps fermented liquid, 5~30mg/L chitosan oligosaccharide
With 5~50mg/L chitosan grape saccharide complex；
(3) cordyceps sinensis of overlay film is packed into packaging bag, is filled with mixed gas and carries out controlled atmospheric packing, obtains controlled atmospheric packing
Cordyceps sinensis refrigerates the cordyceps sinensis of controlled atmospheric packing；
The mixed gas is 85~95:3~8:2~7:0~5 argon gas, oxygen, carbon dioxide and nitrogen by volume ratio
Composition.
Preferably, in step (1), the fresh cordyceps sinensis is soaked in fresh-keeping liquid again by ultrasonic cleaning.
Preferably, the clear power of the ultrasound is 250~1200W, and the frequency of ultrasonic cleaning is 40kHz~120kHz, is surpassed
The number of sound cleaning is 2~5 times, and the time being cleaned by ultrasonic every time independently is 5~10min.
Preferably, in step (1), the preparation method of the ethanol extract of the Cordyceps fermented liquid includes following step
It is rapid:
S1, cordyceps sinensis is inoculated in liquid fermentation medium, cultivates 6~8d, be separated by solid-liquid separation, obtain cordyceps sinensis hair
Zymotic fluid；
S2, the concentration expressed in percentage by volume that ethyl alcohol to ethyl alcohol are added into Cordyceps fermented liquid reach 70~80%, are centrifuged
Supernatant removes drying after ethyl alcohol, obtains the ethanol extract of Cordyceps fermented liquid.
Preferably, the liquid fermentation liquid in the step S1 includes potato, soya bean, sucrose, glucose, peptone, ferment
One of female cream, mineral salt and vitamin are a variety of.
Preferably, in step (1), the preparation method of the chitosan grape saccharide complex the following steps are included:
A1, chitosan and glucose are dissolved in glacial acetic acid, are configured to containing 0.5~2% chitosan of volumetric concentration and body
The glacial acetic acid solution of product 0.5~2% glucose of concentration；
A2, the glacial acetic acid solution containing 0.5~2% glucose of 0.5~2% chitosan of volumetric concentration and volumetric concentration is existed
103.4KPa, 15~20min is reacted under conditions of 121.3 DEG C, freeze-drying obtains chitosan grape saccharide complex.
Preferably, the soaking time in the step (1) is 2~5min.
Preferably, the soaking time in the step (2) is 30~200s.
Preferably, the refrigerated storage temperature in the step (3) is -10~0 DEG C.
Compared with prior art, beneficial effects of the present invention:
The present invention provides a kind of methods of fresh-keeping cordyceps sinensis of the controlled atmospheric packing containing argon gas, are first protecting fresh cordyceps sinensis
It is impregnated in fresh liquid, is soaked in overlay film liquid again after taking out drying, shows to form layer protecting film in cordyceps sinensis, it will be coated with film
Cordyceps sinensis carries out controlled atmospheric packing with the mixed gas containing argon gas, realizes to the fresh-keeping of cordyceps sinensis.The present invention, which utilizes, to be contained
The mixed gas of argon gas carries out controlled atmospheric packing, can significantly inhibit the weight-loss ratio decline of cordyceps sinensis, slow down soluble protein and contain
The decline of amount, the growth for delaying water activity and polyphenol oxidase activity is delayed to increase, it is fresh-keeping relative to conventional preservation method
Time at least can achieve 75d, keep better effect to the color, smell and taste of fresh cordyceps sinensis.
Specific embodiment
The present invention provides a kind of methods of fresh-keeping cordyceps sinensis of the controlled atmospheric packing containing argon gas, comprising the following steps:
(1) fresh cordyceps sinensis is soaked in fresh-keeping liquid, takes out drying, the cordyceps sinensis after being impregnated；
The fresh-keeping liquid includes 1~3mmol/L D-sorbite, 1-3mmol/L vitamin C or ISOASCORBIC ACID, 6~9 μ
The L-cysteine concentration of mol/L phytic acid, 0.4~0.8mg/kg；
(2) cordyceps sinensis after immersion is soaked in overlay film liquid, takes out drying, obtains the cordyceps sinensis of overlay film；
The overlay film liquid includes the ethanol extract of 30~100mg/ml Cordyceps fermented liquid, 5~30mg/L chitosan oligosaccharide
With 5~50mg/L chitosan grape saccharide complex；
(3) cordyceps sinensis of overlay film is packed into packaging bag, is filled with mixed gas and carries out controlled atmospheric packing, obtains controlled atmospheric packing
Cordyceps sinensis refrigerates the cordyceps sinensis of controlled atmospheric packing；
The mixed gas is 85~95:3~8:2~7:0~5 argon gas, oxygen, carbon dioxide and nitrogen by volume ratio
Composition.
Fresh cordyceps sinensis is soaked in fresh-keeping liquid by the present invention, takes out drying, the cordyceps sinensis after being impregnated.The present invention
Fresh-keeping liquid is prepared to impregnate fresh cordyceps sinensis, to keep the moisture of fresh cordyceps sinensis in preservation process, prevent oxidation, etc. originals
The brown stain because caused by and putrid and deteriorated reduces weight-loss ratio, prevents from softening.Fresh-keeping liquid of the present invention include composite antioxidant and
Preservation is played in compound preservative, the limitation for avoiding the fresh-keeping approach of single antistaling agent single, collaboration.Each original in fresh-keeping liquid
The composition ratio of material is the active function range of component, and controls and add within acceptable dose range in food.
The fresh-keeping liquid that the present invention uses fresh cordyceps sinensis of the present invention refers to that acquisition time is no more than for 24 hours and does not do
Dry cordyceps sinensis.Preservation method of the present invention is to protect the quality of fresh cordyceps sinensis not significantly change.
Fresh cordyceps sinensis of the present invention is preferably cleaned before being soaked in fresh-keeping liquid, and the cleaning agent of the cleaning is excellent
Choosing includes water or calcium lactate solution.The purpose that the present invention cleans fresh cordyceps sinensis be in order to remove the impurity such as silt, not into
Row cleaning can also realize the fresh-keeping effect of preservation method of the present invention.In the present invention, the quality hundred of the calcium lactate solution
Point concentration is preferably 0.1~0.5%, and more preferably 0.2~0.4%.
In the present invention, the method for the cleaning is preferably cleaned by ultrasonic, using ultrasonic wave cavitation in a liquid,
Acceleration effect and direct flow effect to liquid and dirt directly, indirectly effect, make crud layer by dispersion, emulsification, removing and
Reach and rapidly and efficiently clean fresh cordyceps sinensis surface risk pollutant purpose, Conventional cleaning methods is avoided to damage in the process of cleaning
Sample surfaces.Currently preferred, the power of the ultrasonic cleaning is 250~1200W；More preferably 600~900W.The present invention
Preferably, the frequency of the ultrasonic cleaning is preferably 40kHz~120kHz；More preferably 70~80kHz.It is currently preferred,
The number of the ultrasonic cleaning is preferably 2~5 times, and more preferably 3 times；The present invention is it is further preferred that be every time cleaned by ultrasonic
Time independently is 5~10min, is still more preferably 6~8min.
In the present invention, the fresh-keeping liquid includes that 1~3mmol/L D-sorbite, 1~3mmol/L vitamin C or different dimension are raw
The L-cysteine of plain C, 6~9 μm of ol/L phytic acid, 0.4~0.8mg/kg；It preferably include 1.5~2mmol/L D-sorbite, 2
The L-cysteine of~2.5mmol/L vitamin C or ISOASCORBIC ACID, 7~8 μm of ol/L phytic acid, 0.5~0.6mg/kg.
In the present invention, D-sorbite has the function of moisture absorption, water conservation, the D-sorbite in fresh-keeping liquid of the present invention
It is used as water-retaining agent.
In the present invention, the vitamin C or ISOASCORBIC ACID use in fresh-keeping liquid as antioxidant, can keep fresh
The color and natural flavor of cordyceps sinensis, prolong storage period, and without any side effects.
In the present invention, phytic acid uses in fresh-keeping liquid as antioxidant, metal ion chelation agent and antistaling agent.Phytic acid
Hydrolysate is inositol and phosphatide in human body, and inositol has anti-aging effect, and phosphatide is the important component of human body cell.
Meanwhile each matter acid molecule can provide six pairs of hydrogen atoms, make free radical electronically forms rock-steady structure, thus instead of by fresh-keeping
Cordyceps sinensis is used as oxygen molecule, avoids fresh cordyceps sinensis oxidation deterioration.
In the present invention, L-cysteine uses in fresh-keeping liquid as BPH resistant rice variety antistaling agent, to prevent vitamin C or different
Vitamin C oxidation, can also prevent the brown stain of cordyceps sinensis surface.
In the present invention, the time that the fresh cordyceps sinensis impregnates in fresh-keeping liquid is preferably 2~5min, and more preferably 3
~4min.The present invention is not particularly limited the dosage of the fresh-keeping liquid, can submerge fresh cordyceps sinensis.
In the present invention, the temperature of the drying is preferably 0~4 DEG C, and more preferably 2~3 DEG C.The present invention selects 0~4 DEG C
Be dried is in order to avoid causing the corruption of cordyceps sinensis to accelerate and internal moisture loss and nutritional ingredient, heat because of high temperature
The loss of quick property substance.It is not particularly limited in mode of the present invention to the drying, using methods known in the art, example
Such as cold air drying.
After cordyceps sinensis after being impregnated, the cordyceps sinensis after immersion is soaked in overlay film liquid by the present invention, is taken out dry
It is dry, obtain the cordyceps sinensis of overlay film.Overlay film liquid of the present invention can form one layer of antibacterial, oxidation resistant on cordyceps sinensis surface
Protective layer, in addition to can prevent the brown stains caused by reasons such as oxidation, spoilage organisms proliferation and it is putrid and deteriorated while, can also be effectively
The raising for inhibiting its respiratory intensity delays the decline of the nutriments such as soluble solid and titratable acid, reduces weight-loss ratio, prevents
Only soften.
Overlay film liquid of the present invention includes the ethanol extract of 30~100mg/ml Cordyceps fermented liquid, 5~30mg/L
Chitosan oligosaccharide and 5~50mg/L chitosan grape saccharide complex；Preferred includes the ethyl alcohol of 50~80mg/ml Cordyceps fermented liquid
Extract, 10~20mg/L chitosan oligosaccharide and 15~35mg/L chitosan grape saccharide complex.
In the present invention, the principle active component in the ethanol extract of the Cordyceps fermented liquid is guanine, worm
Careless element, hypoxanthine, cytidine, xanthine and adenosine, rise in overlay film liquid of the present invention and use as antimicrobial antistaling agent, inhibit
Bacterial reproduction in preservation process.
Specifically, the ethanol extract of Cordyceps fermented liquid of the present invention is preferably according to the side included the following steps
It is prepared by method:
S1, cordyceps sinensis is inoculated in liquid fermentation medium, cultivates 6~8d, be separated by solid-liquid separation, obtain cordyceps sinensis hair
Zymotic fluid；
S2, the concentration expressed in percentage by volume that ethyl alcohol to ethyl alcohol are added into Cordyceps fermented liquid reach 70~80%, are centrifuged
Supernatant removes drying after ethyl alcohol, obtains the ethanol extract of Cordyceps fermented liquid.
In the present invention, the liquid fermentation medium preferably includes but is not limited to potato, soya bean, sucrose, grape
One of sugar, peptone, yeast extract, mineral salt and vitamin are a variety of.In the present invention, the cordyceps sinensis is inoculated with liquid
The inoculum concentration of fermentation medium is preferably 0.01~0.1g/ml, more preferably 0.05g/ml.In the present invention, the culture
Time is preferably 6~8d, more preferably 7d.In the present invention, the temperature of the culture is preferably 22~30 DEG C, more preferably 26
℃.The present invention is not particularly limited the solid-liquid separating method, using manner known in the art, such as filtering or centrifugation
Deng.
It is big in order to precipitate removal that it is 70~80% that the present invention, which adds ethyl alcohol to concentration of alcohol into Cordyceps fermented liquid,
The impurity such as molecule protein, polysaccharide, and active principle is enriched in supernatant.In the present invention, the revolving speed of the centrifugation is preferred
For 5000~15000rpm, more preferably 10000rpm.In the present invention, the time of the centrifugation is preferably 15~60min, more
Preferably 30min.The present invention is not particularly limited to how to remove ethyl alcohol, is steamed using method commonly used in the art, such as rotation
Hair.The present invention is not particularly limited the mode of the drying, using methods known in the art, such as heated-air drying, subtracts
It press dry dry.
In the present invention, chitosan uses in overlay film liquid as antisepsis antistaling agent.Chitosan is a kind of natural food
Preservative has stronger antibacterial and antioxidation, additionally it is possible to effectively inhibit the respiratory intensity in winter worm summer to increase, delay solvable
Property the nutriments such as solid content and titratable acid be lost, reduce weight-loss ratio, prevent from softening.
In the present invention, chitosan grape saccharide complex plays to form antibacterial, anti-oxidant, inhibition breathing etc. in overlay film liquid
The effect of the protective film of effect.In the present invention, the preparation method of the chitosan grape saccharide complex preferably includes following
Step:
A1, chitosan and glucose are dissolved in glacial acetic acid, are configured to containing 0.5~2% chitosan of volumetric concentration and body
The glacial acetic acid solution of product 0.5~2% glucose of concentration；
A2, the glacial acetic acid solution containing 0.5~2% glucose of 0.5~2% chitosan of volumetric concentration and volumetric concentration is existed
103.4KPa、121.3℃(1.05kg/cm2) under conditions of react 15~20min, it is multiple to obtain chitosan glucose for freeze-drying
Close object.
In the present invention, in the step A1, the glacial acetic acid solution being preferably configured to contains volumetric concentration 1~1.5%
1~1.5% glucose of chitosan and volumetric concentration.In the present invention, the time reacted in the step A2 is preferably 16~
18min。
In the present invention, the time that the cordyceps sinensis of the immersion impregnates in overlay film liquid is preferably 30~200s, more excellent
It is selected as 80~150s.The present invention is not particularly limited the dosage of the overlay film liquid, can submerge cordyceps sinensis.In the present invention
In, the temperature of the drying is preferably 0~4 DEG C, and more preferably 2~3 DEG C.
In the present invention, the solvent of the antistaling agent and filming agent is preferably water.
After obtaining the cordyceps sinensis of overlay film, the cordyceps sinensis of overlay film is packed into packaging bag by the present invention, be filled with mixed gas into
Row controlled atmospheric packing obtains the cordyceps sinensis of controlled atmospheric packing, and the cordyceps sinensis of controlled atmospheric packing is refrigerated.
Mixed gas of the present invention is 85~95:3~8:2~7:0~5 argon gas, oxygen, carbon dioxide by volume ratio
It is formed with nitrogen.The present invention is fresh-keeping to cordyceps sinensis progress controlled atmospheric packing using the mixed gas containing argon gas, provides and is directed to
The mixed gas formula of cordyceps sinensis long acting fresh-keeping delays soluble egg to reduce the weightlessness of cordyceps sinensis during preservation
White loss, polyphenol oxidase activity increase, water activity increases.
In the present invention, the material of the packaging bag includes but is not limited to PET/CPE, LDPE or PEIPA/EV OH/PA/
MPE.The realization of controlled atmospheric packing of the present invention uses the device of controlled atmospheric packing, and which is not limited by the present invention.
In the present invention, the temperature of the refrigeration is preferably -10~0 DEG C, more preferably -8~-4 DEG C.
The method of the fresh-keeping cordyceps sinensis of irradiation method provided by the invention combines composite preservative, covers for cordyceps sinensis surface
The processing of film and the controlled atmospheric packing containing argon gas can effectively extend the fresh-keeping of cordyceps sinensis, delay the mistake of cordyceps sinensis fresh goods
Rate growth, the reduction of soluble protein, the growth of polyphenol oxidase activity and the growth of water activity again.
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
Embodiment 1
Prepare fresh-keeping liquid: the different Vc of 1mmol/L D-sorbite, 1mmol/L, 6 μm of ol/L phytic acid, L-cysteine concentration are
0.4mg/kg。
The preparation of the ethanol extract of Cordyceps fermented liquid: liquid fermentation training is inoculated in after aweto strain is activated
It supports in base (including potato, soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin), is cultivated at 26 DEG C
7d, centrifuging and taking supernatant, obtains Cordyceps fermented liquid；The volume basis of ethyl alcohol to ethyl alcohol is added into Cordyceps fermented liquid
Concentration stops when being 75%, and supernatant, the ethyl alcohol in vacuum distillation removal supernatant is collected by centrifugation, and freeze-drying obtains winter worm
The ethanol extract of summer grass fermentation liquid.
The preparation of chitosan grape saccharide complex: 1% chitosan of configuration quality percentage concentration, 1% Portugal of mass percentage concentration
The glacial acetic acid mixed solution of grape sugar, in pressure 103.4kPa (1.05kg/cm2), maintain 15 minutes under the conditions of 121.3 DEG C of temperature,
Reactant freeze-drying, obtains chitosan grape saccharide complex.
Prepare overlay film liquid: 30mg/mL cordyceps mycelia extract, 5mg/mL chitosan oligosaccharide, 5mg/mL chitosan glucose are multiple
Close object.
At 0 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production according to
It is secondary to be cleaned；It is ultrasound in 0.1~0.5.% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration,
Wave cleans 5 times (250W, frequency 120Hz, 0 DEG C of temperature, time 5min/ times), and 0 DEG C drains surface moisture.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 2min, 0 DEG C of cold wind drying, the winter after being impregnated in fresh-keeping liquid
Worm summer grass.Cordyceps sinensis after immersion is impregnated into 30s in overlay film liquid, 0 DEG C of cold wind drying obtains the cordyceps sinensis of overlay film.
The cordyceps sinensis of overlay film is fitted into packaging bag made of PET/CPE material, it will be by argon gas, oxygen with gas-control packing device
The mixed gas of gas, carbon dioxide and nitrogen volume ratio 85:5:5:5 is filled in packaging bag, obtains the cordyceps sinensis of controlled atmospheric packing.
It is refrigerated at -10 DEG C.
Embodiment 2
Prepare fresh-keeping liquid: 3mmol/L D-sorbite, 3mmol/LVc, 9 μm of ol/L phytic acid, L-cysteine concentration are
0.8mg/kg。
The preparation of the ethanol extract of Cordyceps fermented liquid: liquid fermentation training is inoculated in after aweto strain is activated
It supports in base (including potato, soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin), is cultivated at 25 DEG C
7d, centrifuging and taking supernatant, obtains Cordyceps fermented liquid；The volume basis of ethyl alcohol to ethyl alcohol is added into Cordyceps fermented liquid
Concentration stops when being 70%, and supernatant, the ethyl alcohol in vacuum distillation removal supernatant is collected by centrifugation, and freeze-drying obtains winter worm
The ethanol extract of summer grass fermentation liquid.
The preparation of chitosan grape saccharide complex: 1% chitosan of configuration quality percentage concentration, 1% Portugal of mass percentage concentration
The glacial acetic acid mixed solution of grape sugar, in pressure 103.4kPa (1.05kg/cm2), maintain 20 minutes under the conditions of 121.3 DEG C of temperature,
Reactant freeze-drying, obtains chitosan grape saccharide complex.
Prepare overlay film liquid: 100mg/mL cordyceps mycelia extract, 30mg/mL chitosan oligosaccharide, 50mg/mL chitosan glucose
Compound.
At 4 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production according to
It is secondary to be cleaned；It is ultrasound in 0.1~0.5% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration,
Wave cleans 1 time (power 1200W, frequency 40k HzHz, 0 DEG C of temperature, time 10min/ times), and 4 DEG C drain surface moisture.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 5min, 10 DEG C of cold wind dryings, after being impregnated in fresh-keeping liquid
Cordyceps sinensis.Cordyceps sinensis after immersion is impregnated into 200s in overlay film liquid, 10 DEG C of cold wind dryings obtain the worm summer in winter of overlay film
Grass.
The cordyceps sinensis of overlay film is fitted into packaging bag made of LDPE material, it will be by argon gas, oxygen with gas-control packing device
It is filled in packaging bag with the mixed gas of carbon dioxide volume ratio 95:3:2, obtains the cordyceps sinensis of controlled atmospheric packing.It is cold at 0 DEG C
Hiding.
Embodiment 3
Prepare fresh-keeping liquid: the different Vc of 2mmol/L D-sorbite, 2mmol/L, 7 μm of ol/L phytic acid, L-cysteine concentration are
0.6mg/kg。
The preparation of the ethanol extract of Cordyceps fermented liquid: liquid fermentation training is inoculated in after aweto strain is activated
It supports in base (including potato, soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin), is cultivated at 27 DEG C
7d, centrifuging and taking supernatant, obtains Cordyceps fermented liquid；The volume basis of ethyl alcohol to ethyl alcohol is added into Cordyceps fermented liquid
Concentration stops when being 80%, and supernatant, the ethyl alcohol in vacuum distillation removal supernatant is collected by centrifugation, and freeze-drying obtains winter worm
The ethanol extract of summer grass fermentation liquid.
The preparation of chitosan grape saccharide complex: 1% chitosan of configuration quality percentage concentration, 1% Portugal of mass percentage concentration
The glacial acetic acid mixed solution of grape sugar, in pressure 103.4kPa (1.05kg/cm2), maintain 16 minutes under the conditions of 121.3 DEG C of temperature,
Reactant freeze-drying, obtains chitosan grape saccharide complex.
Prepare overlay film liquid: 70mg/mL cordyceps mycelia extract, 20mg/mL chitosan oligosaccharide, 30mg/mL chitosan glucose
Compound.
At 5 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production according to
It is secondary to be cleaned；It is ultrasonic cleaning in 0.1% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration,
3 times (power 700W, frequency 80Hz, 5 DEG C of temperature, time 7min/ times), 2 DEG C drain surface moisture.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 3min, 2 DEG C of cold wind dryings, the winter after being impregnated in fresh-keeping liquid
Worm summer grass.Cordyceps sinensis after immersion is impregnated into 150s in overlay film liquid, 0 DEG C of cold wind drying obtains the cordyceps sinensis of overlay film.
The cordyceps sinensis of overlay film is fitted into packaging bag made of PEIPA/EV OH/PA/MPE material, with gas-control packing device
It will be filled in packaging bag by the mixed gas of argon gas, oxygen and carbon dioxide volume ratio 90:5:5, obtain the winter worm of controlled atmospheric packing
Summer grass.It is refrigerated at -5 DEG C.
Embodiment 4
Prepare fresh-keeping liquid: 1mmol/L D-sorbite, 3mmol/LVc, 8 μm of ol/L phytic acid, L-cysteine concentration are
0.7mg/kg。
The preparation of the ethanol extract of Cordyceps fermented liquid: liquid fermentation training is inoculated in after aweto strain is activated
It supports in base (including potato, soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin), is cultivated at 24 DEG C
7d, centrifuging and taking supernatant, obtains Cordyceps fermented liquid；The volume basis of ethyl alcohol to ethyl alcohol is added into Cordyceps fermented liquid
Concentration stops when being 80%, and supernatant, the ethyl alcohol in vacuum distillation removal supernatant is collected by centrifugation, and freeze-drying obtains winter worm
The ethanol extract of summer grass fermentation liquid.
The preparation of chitosan grape saccharide complex: 1% chitosan of configuration quality percentage concentration, 1% Portugal of mass percentage concentration
The glacial acetic acid mixed solution of grape sugar, in pressure 103.4kPa (1.05kg/cm2), maintain 16 minutes under the conditions of 121.3 DEG C of temperature,
Reactant freeze-drying, obtains chitosan grape saccharide complex.
Prepare overlay film liquid: 70mg/mL cordyceps mycelia extract, 20mg/mL chitosan oligosaccharide, 30mg/mL chitosan glucose
Compound.
At 4 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production according to
It is secondary to be cleaned；It is ultrasonic cleaning in 0.1% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration,
4 times (power 400W, frequency zones 100Hz, 4 DEG C of temperature, time 6min/ times), 2 DEG C drain surface moisture.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 4min, 2 DEG C of cold wind dryings, the winter after being impregnated in fresh-keeping liquid
Worm summer grass.Cordyceps sinensis after immersion is impregnated into 180s in overlay film liquid, 3 DEG C of cold wind dryings obtain the cordyceps sinensis of overlay film.
The cordyceps sinensis of overlay film is fitted into packaging bag made of PET/CPE material, it will be by argon gas, oxygen with gas-control packing device
The mixed gas of gas, carbon dioxide and nitrogen volume ratio 92:3:3:2 is filled in packaging bag, obtains the cordyceps sinensis of controlled atmospheric packing.
It is refrigerated at -2 DEG C.
Embodiment 5
Prepare fresh-keeping liquid: 1.5mmol/L D-sorbite, 2.5mmol/LVc, 7.5 μm of ol/L phytic acid, L-cysteine concentration
For 0.65mg/kg.
The preparation of the ethanol extract of Cordyceps fermented liquid: liquid fermentation training is inoculated in after aweto strain is activated
It supports in base (including potato, soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin), is cultivated at 28 DEG C
7d, centrifuging and taking supernatant, obtains Cordyceps fermented liquid；The volume basis of ethyl alcohol to ethyl alcohol is added into Cordyceps fermented liquid
Concentration stops when being 77%, and supernatant, the ethyl alcohol in vacuum distillation removal supernatant is collected by centrifugation, and freeze-drying obtains winter worm
The ethanol extract of summer grass fermentation liquid.
The preparation of chitosan grape saccharide complex: 1% chitosan of configuration quality percentage concentration, 1% Portugal of mass percentage concentration
The glacial acetic acid mixed solution of grape sugar, in pressure 103.4kPa (1.05kg/cm2), maintain 18 minutes under the conditions of 121.3 DEG C of temperature,
Reactant freeze-drying, obtains chitosan grape saccharide complex.
Prepare overlay film liquid: 85mg/mL cordyceps mycelia extract, 17mg/mL chitosan oligosaccharide, 22mg/mL chitosan glucose
Compound.
At 9 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production according to
It is secondary to be cleaned；It is ultrasonic cleaning in 0.1% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration,
2 times (power 750W, frequency zones 80Hz, 6 DEG C of temperature, time 7min/ times), 1 DEG C drains surface moisture.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 5min, 1 DEG C of cold wind drying, the winter after being impregnated in fresh-keeping liquid
Worm summer grass.Cordyceps sinensis after immersion is impregnated into 70s in overlay film liquid, 1 DEG C of cold wind drying obtains the cordyceps sinensis of overlay film.
The cordyceps sinensis of overlay film is fitted into packaging bag made of PEIPA/EV OH/PA/MPE material, with gas-control packing device
It will be filled in packaging bag by the mixed gas of argon gas, oxygen, carbon dioxide and nitrogen volume ratio 88:5:5:2, obtain controlled atmospheric packing
Cordyceps sinensis.It is refrigerated at -8 DEG C.
Comparative example 1
" storing and fresh-keeping method of fresh cordyceps sinensis " recorded according to Chinese patent 201611222582.3 is to the fresh winter worm summer
Grass carries out fresh-keeping:
The fresh cordyceps sinensis just excavated is taken, is cleaned under 5 DEG C of environment temperatures with 5 DEG C of water respectively, then in 8 DEG C of environment
Under be less than 0.5g, 0.5~1.0g by weight, fall into three classes greater than 1.0g.
Cordyceps sinensis by weight less than 0.5g saves under -5 DEG C, 75% damp condition；
The cordyceps sinensis of 0.5~1.0g of weight is saved under -7 DEG C, 70% damp condition；
Cordyceps sinensis by weight greater than 1.0g saves under -10 DEG C, 60% damp condition.
Embodiment 6
1, process is tested
Take fresh cordyceps sinensis 180 of 0.5~1.0g specification respectively, be randomly divided into 6 groups, respectively according to Examples 1 to 5 and
The method of comparative example 1 is fresh-keeping to the progress of each group fresh cordyceps sinensis, and measurement each group cordyceps sinensis is relative to Preservation Treatment after saving 75d
Preceding weight-loss ratio, soluble protein content rate of descent, polyphenol oxidase activity growth rate and water activity growth rate.Testing result
As shown in table 1.
Sample weight loss rate is according to " Wang Jingbo waits influence [J] of .3 kind plants essential oil suffocating treatment to nectarine fresh-keeping effect
Nuclear agricultural science report, 2018,32 (5): the method measurement in 933-940. "；Soluble protein content rate of descent is according to " Deng Lili waits
Bright trip's method measurement condition optimizing [J] the Food Science of apple tissue trace soluble egg from content of coomassie, 2012, (24):
185-189. " method measurement；Polyphenol oxidase activity growth rate is according to " Jiankang waits postharvest fruit and vegetable bio-chemical characteristics to instruct
Beijing [M]: China Light Industry Press, the measurement of 2007:59-154. " method；Water activity is surveyed using water activity detecting instrument
It is fixed.
The results are shown in Table 1, when saving the 75th day, the fresh-keeping cordyceps sinensis of the method for the present invention, in weight-loss ratio, solubility
In terms of protein content rate of descent and polyphenol oxidase activity growth rate, water activity growth rate, it is superior to Chinese patent
201611222582.3 the fresh-keeping cordyceps sinensis of method.Meanwhile the fresh-keeping effect of the method for the invention is with fresh keeping time
Extension advantage is more obvious.
Table 1 saves the 75th day, weight-loss ratio, soluble protein content rate of descent and the polyphenol of each processing group cordyceps sinensis sample
Oxidase active growth rate, water activity growth rate measurement result
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of method of the fresh-keeping cordyceps sinensis of controlled atmospheric packing containing argon gas, comprising the following steps:
(1) fresh cordyceps sinensis is soaked in fresh-keeping liquid, takes out drying, the cordyceps sinensis after being impregnated；
The fresh-keeping liquid includes 1~3mmol/L D-sorbite, 1-3mmol/L vitamin C or ISOASCORBIC ACID, 6~9 μm of ol/L plants
The L-cysteine concentration of acid, 0.4~0.8mg/kg；
(2) cordyceps sinensis after immersion is soaked in overlay film liquid, takes out drying, obtains the cordyceps sinensis of overlay film；
The overlay film liquid include the ethanol extract of 30~100mg/ml Cordyceps fermented liquid, 5~30mg/L chitosan oligosaccharide and 5~
50mg/L chitosan grape saccharide complex；
(3) cordyceps sinensis of overlay film is packed into packaging bag, is filled with mixed gas and carries out controlled atmospheric packing, obtains the winter worm of controlled atmospheric packing
Summer grass, the cordyceps sinensis of controlled atmospheric packing is refrigerated；
The mixed gas is 85~95:3~8:2~7:0~5 argon gas, oxygen, carbon dioxide and nitrogen group by volume ratio
At.
2. the method according to claim 1, wherein the fresh cordyceps sinensis is by ultrasonic cleaning in step (1)
It is soaked in fresh-keeping liquid again.
3. according to the method described in claim 2, ultrasound is clear it is characterized in that, the clear power of the ultrasound is 250~1200W
The frequency washed is 40kHz~120kHz, and the number of ultrasonic cleaning is 2~5 times, the time being cleaned by ultrasonic every time independently is 5~
10min。
4. the method according to claim 1, wherein the ethyl alcohol of the Cordyceps fermented liquid mentions in step (1)
Take the preparation method of object the following steps are included:
S1, cordyceps sinensis is inoculated in liquid fermentation medium, cultivates 6~8d, be separated by solid-liquid separation, obtain cordyceps sinensis fermentation
Liquid；
S2, the concentration expressed in percentage by volume that ethyl alcohol to ethyl alcohol are added into Cordyceps fermented liquid reach 70~80%, are centrifuged to obtain supernatant
Liquid removes drying after ethyl alcohol, obtains the ethanol extract of Cordyceps fermented liquid.
5. according to the method described in claim 4, it is characterized in that, the liquid fermentation liquid in the step S1 includes including Ma Ling
One of potato, soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin are a variety of.
6. the method according to claim 1, wherein in step (1), the system of the chitosan grape saccharide complex
Preparation Method the following steps are included:
A1, chitosan and glucose are dissolved in glacial acetic acid, are configured to dense containing 0.5~2% chitosan of volumetric concentration and volume
Spend the glacial acetic acid solution of 0.5~2% glucose；
A2, the glacial acetic acid solution containing 0.5~2% glucose of 0.5~2% chitosan of volumetric concentration and volumetric concentration is existed
103.4KPa, 15~20min is reacted under conditions of 121.3 DEG C, freeze-drying obtains chitosan grape saccharide complex.
7. the method according to claim 1, wherein the soaking time in the step (1) is 2~5min.
8. method according to claim 1 or claim 7, which is characterized in that soaking time in the step (2) is 30~
200s。
9. the method according to claim 1, wherein the refrigerated storage temperature in the step (3) is -10~0 DEG C.
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