CN115957245B - Method for processing nutrition of cordyceps sinensis fresh-keeping lock by cascade treatment - Google Patents
Method for processing nutrition of cordyceps sinensis fresh-keeping lock by cascade treatment Download PDFInfo
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Abstract
The application discloses a method for processing nutrition of a combined-stage processed cordyceps sinensis fresh-keeping lock, which comprises the steps of cleaning, fresh-keeping pretreatment, stabilizing, modified atmosphere packaging and sterilization, and belongs to the technical field of cordyceps sinensis fresh-keeping, in particular to a method for processing nutrition of a combined-stage processed cordyceps sinensis fresh-keeping lock; in order to solve the problem that the prior art still cannot realize the effective, convenient and low-cost fresh-keeping and fresh-keeping effects on the cordyceps sinensis, the application provides a method for preprocessing the natural fresh-keeping composition, which can inhibit the breathing effect of the cordyceps sinensis, reduce the activity of polyphenol oxidase, reduce the loss of nutrition matters of the cordyceps sinensis in the storage process, improve the refrigerating and fresh-keeping effects on the cordyceps sinensis, simplify the storage environment, remarkably prolong the storage and fresh-keeping time of the cordyceps sinensis and have wide application prospects.
Description
Technical Field
The application belongs to the technical field of cordyceps sinensis preservation, and particularly relates to a nutrition processing method for a cascade treatment cordyceps sinensis preservation lock.
Background
Cordyceps sinensis is a complex of stroma and larva corpse of Cordyceps sinensis of Clavipitaceae on larva of Hepialidae, is a traditional rare nourishing Chinese medicinal material, has various effects of regulating immune system function, resisting tumor, resisting fatigue and the like, and is mostly dried in the sun at present, and since the Cordyceps sinensis contains a plurality of thermosensitive nutritional components, the sun-dried Cordyceps sinensis volatilizes sunlight, so that the nutritional components of the Cordyceps sinensis are reduced, and the fresh keeping and freshness locking of fresh Cordyceps sinensis are very important.
However, fresh cordyceps sinensis is preserved for a short period of time at zero temperature, and is preserved by freezing at minus temperature, but the freezing preservation has the problems of high cost, nutrition loss, inconvenient transportation, easy softening after thawing and bad taste, so that the development of the cordyceps sinensis refrigeration technology has important significance, and Chinese patent CN110628633B discloses a fresh-keeping method for the fresh cordyceps sinensis and a fresh cordyceps sinensis product, which realize the fresh-keeping effect on the cordyceps sinensis for 90-105 days under the condition of being above 0 ℃, but the technology has good fresh-keeping effect only under the windless environment, and most of the existing household storage tools rely on cold air circulation refrigeration, the diffusion of gas in a package is accelerated by the flow of air, the breathing effect of the cordyceps sinensis is enhanced, and the storage time of the cordyceps sinensis is reduced, so that the effective, convenient and low-cost fresh-keeping effect on the cordyceps sinensis cannot be realized in the prior art.
Disclosure of Invention
1.1 purpose
Aiming at the situation, the application provides a method for processing the nutrition of the cordyceps sinensis fresh-keeping lock by cascade treatment in order to overcome the defects of the prior art, and the application provides a method for processing the nutrition of the cordyceps sinensis fresh-keeping lock by cascade pretreatment in order to solve the problem that the prior art still cannot realize the effective, convenient and low-cost fresh-keeping lock effect of cordyceps sinensis.
1.2 detailed description of the application
Specifically, in order to achieve the purpose, the application provides a combined-stage processing cordyceps sinensis fresh-keeping lock nutrition processing method which comprises the steps of cleaning, fresh-keeping pretreatment, stabilizing, modified atmosphere packaging and sterilization, wherein the cleaning is to clean and remove impurities such as muddy sand attached to the surface of fresh cordyceps sinensis by using water, the fresh-keeping pretreatment is to soak cordyceps sinensis in a natural fresh-keeping composition, take out and dry the cordyceps sinensis to obtain soaked cordyceps sinensis, the stabilizing is to place the cordyceps sinensis in a nitrogen closed container with the temperature of 0-4 ℃ for 5-8 hours, and the modified atmosphere packaging is to fill the cordyceps sinensis after stabilizing into packaging materials, and fill packaging gas into the packaging materials for modified atmosphere packaging; the sterilization is carried out for 1-2 hours under ultraviolet light to obtain sterilized Cordyceps sinensis processed product, and storing Cordyceps sinensis processed product at 0-4deg.C.
Further, the cleaning can be performed by adopting ultrasonic cleaning for a plurality of times at a low frequency, wherein the power of the ultrasonic cleaning is 550-1200W, the frequency of the ultrasonic cleaning is 35-60kHz, the times of the ultrasonic cleaning are 5-8 times, and the time of the ultrasonic cleaning is 3-5 minutes/time.
Further, the fresh-keeping pretreatment time is 3-5 hours, and the temperature of the fresh-keeping pretreatment is 0-4 ℃.
Preferably, stirring is continuously carried out in the fresh-keeping pretreatment process, the stirring frequency is 5-10 revolutions per minute, the stirring is used for promoting the soaking effect of the natural fresh-keeping composition and the cordyceps sinensis, and the natural fresh-keeping composition has the same effect without stirring.
Further, the natural fresh-keeping composition comprises the following components in parts by weight: 10-15 parts of echinacea purpurea root extract, 5-10 parts of goose phyllostachys pubescens leaf extract, 1-5 parts of secoisolariciresinol diglucoside, 0.5-3 parts of vanillic acid and the balance of water.
Preferably, the natural fresh-keeping composition comprises the following components in parts by weight: 12-15 parts of echinacea purpurea root extract, 8-10 parts of goose phyllostachys pubescens leaf extract, 1-3 parts of secoisolariciresinol diglucoside, 0.5-2 parts of vanillic acid and the balance of water.
Further, the packaging gas is composed of 5% -15% v/v of oxygen, 2% -5% v/v of carbon dioxide and the balance of nitrogen, and the volume of the packaging gas is not less than 2.5 times of the volume of the cordyceps sinensis.
Preferably, the packaging material has a water vapor transmission rate of 60-80 g/(m) 2 * 24h) The oxygen transmission rate is 30-70m 3 /(m 2 *24h*0.1MPa)；
Or the packaging material has a water vapor transmission rate of 65 g/(m) 2 * 24h) Oxygen transmission of 40m 3 /(m 2 *24h*0.1MPa)；
Or the packaging material has a water vapor transmission rate of 80 g/(m) 2 * 24h) Oxygen transmission of 70m 3 /(m 2 *24h*0.1MPa)。
In the application, the packaging material is preferably a P30S modified atmosphere box, and other modified atmosphere boxes may have the same effect.
Further, the preparation method of the echinacea purpurea root extract comprises the following steps: weighing 2-4g of dried Echinacea purpurea root, soaking in 80% ethanol, extracting with ultrasonic wave for 3 times, mixing filtrates, concentrating under reduced pressure, and oven drying at 45deg.C.
Further, the preparation method of the goose phyllostachys pubescens leaf extract comprises the following steps: weighing She Ganpin-10 g of goose moso bamboo, soaking in 80% ethanol, extracting with ultrasonic method, repeating for 3 times, mixing filtrates, concentrating under reduced pressure, and oven drying at 45deg.C.
Preferably, the frequency of ultrasonic extraction is 40kHz, the ultrasonic extraction time is 1.5-2 hours, and the ultrasonic extraction temperature is 55 ℃.
1.3 the beneficial effects obtained by the application
In order to solve the problem that the prior art cannot realize the effective, convenient and low-cost fresh-keeping and fresh-keeping effects on cordyceps sinensis, the application provides a method for processing the nutrition of the fresh-keeping lock of the cascade pretreatment cordyceps sinensis;
the natural fresh-keeping composition prepared by screening natural substances can obviously improve the fresh-keeping effect of Cordyceps sinensis after treatment;
the polyphenol oxidase is a main enzyme for enzymatic browning of edible fungi, can cause browning and even spoiling of products, seriously damages the nutritive value, flavor and appearance texture of the edible fungi, and shows that the lower the polyphenol oxidase activity is, the better the storability is, and the application proposes a mode of preprocessing plant extracts, thereby realizing the effects of inhibiting the respiration of the cordyceps sinensis, reducing the polyphenol oxidase activity, reducing the loss of nutrient substances of the cordyceps sinensis in the storage process, and improving the refrigerating, fresh-keeping and freshness-locking effects of the cordyceps sinensis;
in addition, the soluble protein participates in the regulation and control of various physiological and biochemical metabolic processes of the edible fungi, is one of important indexes of the quality and nutrition of the edible fungi, and the content reduction of the soluble protein is considered as an important characteristic of aging of the sampled samples, and can inhibit the reduction of the soluble protein after being treated by the natural fresh-keeping composition along with the extension of the storage time;
the cavitation, acceleration and direct flow of ultrasonic wave in liquid are utilized to directly and indirectly act on liquid and dirt, so that the dirt layer is dispersed, emulsified and stripped to achieve the aim of quickly and efficiently cleaning the surface risk pollutants of fresh cordyceps sinensis, and the surface damage of a sample in the cleaning process of the traditional cleaning method is avoided;
the natural fresh-keeping composition used in the application is easy to prepare and is derived from plants, has wide application prospect, and the method for processing the fresh-keeping lock nutrition of the cascade pretreatment cordyceps sinensis simplifies the storage environment and remarkably prolongs the storage and fresh-keeping time of the cordyceps sinensis.
Drawings
FIG. 1 is a graph showing the analysis result of the weight loss rate after preservation and fresh-keeping by using the nutrition processing method of the Cordyceps sinensis fresh-keeping lock provided by the application;
FIG. 2 is a graph showing the analysis result of the content reduction rate of soluble protein after preservation and fresh-keeping by using the nutrition processing method of the Cordyceps sinensis fresh-keeping lock provided by the application;
FIG. 3 is a graph showing the analysis result of the activity increase rate of polyphenol oxidase after preservation and fresh-keeping by using the nutrition processing method of the Cordyceps sinensis fresh-keeping lock provided by the application.
The accompanying drawings are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate the application and together with the embodiments of the application, serve to explain the application.
Detailed Description
The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the application; all other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition, any methods and materials similar or equivalent to those described herein can be used in the present application. The preferred methods and materials described herein are illustrative only and should not be construed as limiting the application.
The experimental methods in the following examples are all conventional methods unless otherwise specified; the test materials and test strains used in the examples described below, unless otherwise specified, were commercially available.
Example 1
The application provides a combined-stage treatment cordyceps sinensis fresh-keeping lock nutrition processing method which comprises the steps of cleaning, fresh-keeping pretreatment, stabilizing, modified atmosphere packaging and sterilization, wherein the cleaning is to clean and remove impurities such as muddy sand attached to the surface of fresh cordyceps sinensis by using water, the fresh-keeping pretreatment is to soak cordyceps sinensis in a natural fresh-keeping composition, take out and dry the cordyceps sinensis to obtain soaked cordyceps sinensis, the stabilizing is to place the cordyceps sinensis in a nitrogen closed container with the temperature of 0-4 ℃ for 5-8 hours, and the modified atmosphere packaging is to fill the stabilized cordyceps sinensis into packaging materials, and fill packaging gas for modified atmosphere packaging; the sterilization is to sterilize for 1.5 hours under ultraviolet light to obtain sterilized Cordyceps sinensis processed product, and store Cordyceps sinensis processed product at 0-4deg.C.
The ultrasonic cleaning is carried out for a plurality of times at low frequency, the power of the ultrasonic cleaning is 600W, the frequency of the ultrasonic cleaning is 45kHz, the times of the ultrasonic cleaning are 5 times, and the time of the ultrasonic cleaning is 3 minutes/time.
The fresh-keeping pretreatment time is 4 hours, the temperature of the fresh-keeping pretreatment is 0-4 ℃, preferably, stirring is continuously carried out in the fresh-keeping pretreatment process, the stirring frequency is 5-10 revolutions per minute, and the stirring is used for promoting the soaking effect of the natural fresh-keeping composition and the cordyceps sinensis.
The natural fresh-keeping composition comprises the following components in parts by weight: 12 parts of echinacea purpurea root extract, 8 parts of goose phyllostachys pubescens leaf extract, 1 part of secoisolariciresinol diglucoside, 0.5 part of vanillic acid and the balance of water.
The packaging gas consists of 10% v/v oxygen, 5% v/v carbon dioxide and the balance nitrogen, the volume of the packaging gas is not less than 2.5 times of the volume of Cordyceps sinensis, and the water vapor permeability of the packaging material is 65 g/(m) 2 * 24h) Oxygen transmission of 40m 3 /(m 2 *24h*0.1MPa)。
The packaging material is a P30S modified atmosphere box, and the P30S modified atmosphere box is purchased from Shanghai preferred packaging technology limited company.
The preparation method of the echinacea purpurea root extract comprises the following steps: weighing dried Echinacea purpurea root 4g, soaking in 80% ethanol, extracting with ultrasound for 3 times, mixing filtrates, concentrating under reduced pressure, and oven drying at 45deg.C.
The preparation method of the goose phyllostachys pubescens leaf extract comprises the following steps: weighing She Ganpin g of goose moso bamboo, soaking in 80% ethanol, extracting with ultrasonic method for 3 times, mixing filtrates, concentrating under reduced pressure, and oven drying at 45deg.C.
The frequency of ultrasonic extraction is 40kHz, the ultrasonic extraction time is 2 hours, and the ultrasonic extraction temperature is 55 ℃.
Example 2
The application provides a combined-stage treatment cordyceps sinensis fresh-keeping lock nutrition processing method which comprises the steps of cleaning, fresh-keeping pretreatment, stabilizing, modified atmosphere packaging and sterilization, wherein the cleaning is to clean and remove impurities such as muddy sand attached to the surface of fresh cordyceps sinensis by using water, the fresh-keeping pretreatment is to soak cordyceps sinensis in a natural fresh-keeping composition, take out and dry the cordyceps sinensis to obtain soaked cordyceps sinensis, the stabilizing is to place the cordyceps sinensis in a nitrogen closed container with the temperature of 0-4 ℃ for 5-8 hours, and the modified atmosphere packaging is to fill the stabilized cordyceps sinensis into packaging materials, and fill packaging gas for modified atmosphere packaging; the sterilization is to sterilize for 1.5 hours under ultraviolet light to obtain sterilized Cordyceps sinensis processed product, and store Cordyceps sinensis processed product at 0-4deg.C.
The ultrasonic cleaning is carried out for a plurality of times at low frequency, the power of the ultrasonic cleaning is 600W, the frequency of the ultrasonic cleaning is 45kHz, the times of the ultrasonic cleaning are 5 times, and the time of the ultrasonic cleaning is 3 minutes/time.
The fresh-keeping pretreatment time is 4 hours, the temperature of the fresh-keeping pretreatment is 0-4 ℃, preferably, stirring is continuously carried out in the fresh-keeping pretreatment process, the stirring frequency is 5-10 revolutions per minute, and the stirring is used for promoting the soaking effect of the natural fresh-keeping composition and the cordyceps sinensis.
The natural fresh-keeping composition comprises the following components in parts by weight: 12 parts of echinacea purpurea root extract, 8 parts of goose phyllostachys pubescens leaf extract, 1 part of secoisolariciresinol diglucoside, 0.5 part of vanillic acid and the balance of water,
the packaging gas consists of 10% v/v oxygen, 5% v/v carbon dioxide and the balance nitrogen, the volume of the packaging gas is not less than 2.5 times of the volume of Cordyceps sinensis, and the water vapor permeability of the packaging material is 80 g/(m) 2 * 24h) Oxygen transmission of 70m 3 /(m 2 *24h 0.1 MPa), wherein the packaging material is a P30S modified atmosphere box.
The preparation method of the echinacea purpurea root extract comprises the following steps: weighing dried Echinacea purpurea root 4g, soaking in 80% ethanol, extracting with ultrasound for 3 times, mixing filtrates, concentrating under reduced pressure, and oven drying at 45deg.C.
The preparation method of the goose phyllostachys pubescens leaf extract comprises the following steps: weighing She Ganpin g of goose moso bamboo, soaking in 80% ethanol, extracting with ultrasonic method for 3 times, mixing filtrates, concentrating under reduced pressure, and oven drying at 45deg.C.
The frequency of ultrasonic extraction is 40kHz, the ultrasonic extraction time is 2 hours, and the ultrasonic extraction temperature is 55 ℃.
Example 3
The application provides a combined-stage treatment cordyceps sinensis fresh-keeping lock nutrition processing method which comprises the steps of cleaning, fresh-keeping pretreatment, stabilizing, modified atmosphere packaging and sterilization, wherein the cleaning is to clean and remove impurities such as muddy sand attached to the surface of fresh cordyceps sinensis by using water, the fresh-keeping pretreatment is to soak cordyceps sinensis in a natural fresh-keeping composition, take out and dry the cordyceps sinensis to obtain soaked cordyceps sinensis, the stabilizing is to place the cordyceps sinensis in a nitrogen closed container with the temperature of 0-4 ℃ for 5-8 hours, and the modified atmosphere packaging is to fill the stabilized cordyceps sinensis into packaging materials, and fill packaging gas for modified atmosphere packaging; the sterilization is to sterilize for 1.5 hours under ultraviolet light to obtain sterilized Cordyceps sinensis processed product, and store Cordyceps sinensis processed product at 0-4deg.C.
The ultrasonic cleaning is carried out for a plurality of times at low frequency, the power of the ultrasonic cleaning is 600W, the frequency of the ultrasonic cleaning is 45kHz, the times of the ultrasonic cleaning are 5 times, and the time of the ultrasonic cleaning is 3 minutes/time.
The fresh-keeping pretreatment time is 4 hours, and the temperature of the fresh-keeping pretreatment is 0-4 ℃.
Stirring is continuously carried out in the fresh-keeping pretreatment process, the stirring frequency is 5-10 revolutions per minute, and the stirring is used for promoting the soaking effect of the natural fresh-keeping composition and the cordyceps sinensis.
The natural fresh-keeping composition comprises the following components in parts by weight: 15 parts of echinacea purpurea root extract, 10 parts of goose phyllostachys pubescens leaf extract, 3 parts of secoisolariciresinol diglucoside, 2 parts of vanillic acid and the balance of water.
The packaging gas consists of 10% v/v oxygen, 5% v/v carbon dioxide and the balance nitrogen, the volume of the packaging gas is not less than 2.5 times of the volume of Cordyceps sinensis, and the water vapor permeability of the packaging material is 65 g/(m) 2 * 24h) Oxygen transmission of 40m 3 /(m 2 *24h 0.1 MPa), wherein the packaging material is a P30S modified atmosphere box.
The preparation method of the echinacea purpurea root extract comprises the following steps: weighing dried Echinacea purpurea root 4g, soaking in 80% ethanol, extracting with ultrasound for 3 times, mixing filtrates, concentrating under reduced pressure, and oven drying at 45deg.C.
The preparation method of the goose phyllostachys pubescens leaf extract comprises the following steps: weighing She Ganpin g of goose moso bamboo, soaking in 80% ethanol, extracting with ultrasonic method for 3 times, mixing filtrates, concentrating under reduced pressure, and oven drying at 45deg.C.
The frequency of ultrasonic extraction is 40kHz, and the ultrasonic extraction time is 2 hours.
The temperature of the ultrasonic extraction was 55 ℃.
Comparative example 1
The comparative example is a nutrition processing method of a cascade processing Cordyceps sinensis fresh-keeping lock, which is different from example 1 only in that sterile water is suitable for replacing a natural fresh-keeping composition, and the rest components and the content of the components are the same as those of example 1.
Comparative example 2
The comparative example is a method for processing Cordyceps sinensis fresh-keeping lock nutrition by cascade treatment, which is different from example 1 only in that the natural fresh-keeping composition does not contain Echinacea purpurea root extract, and the rest components and the content of the components are the same as example 1.
Comparative example 3
The comparative example is a combined processing method for Cordyceps sinensis fresh-keeping lock nutrition, which is different from example 1 only in that the natural fresh-keeping composition does not contain the goose Mao bamboo leaf extract, and the rest components and the content of the components are the same as those in example 1.
Comparative example 4
The comparative example is a nutrition processing method of a cascade processing Cordyceps sinensis fresh-keeping lock, which is different from example 1 only in that the natural fresh-keeping composition does not contain secoisolariciresinol diglucoside, and the rest components and the content of the components are the same as those of example 1.
Fresh keeping performance test analysis
Experimental example 1
Basic index scoring in Cordyceps sinensis fresh-keeping process
Selecting 0.5-1.0 g of Cordyceps sinensis, performing fresh-keeping treatment and packaging according to the methods of example 1, example 2 and example 3, storing in environment with temperature of 0-4deg.C and humidity of 60-70%, taking out 10 Cordyceps sinensis at intervals of half month, and evaluating color, hardness and water yield.
As shown in Table 1, the results of the evaluation were that the color, hardness and retention of water in the processing methods of examples 1 to 3 were better, while the results of comparative examples 1 to 4 were not good.
Specifically, the color of the preservation method of the embodiment 1-the embodiment 3 is still yellow and the hardness is slightly soft after 75 days, and only a small amount of water is discharged;
the comparative examples 1 to 4 were all dark yellow and black yellow with different degrees, soft hardness and serious water yield.
TABLE 1 evaluation of color in fresh-keeping storage of Cordyceps sinensis
| Group of | Day 15 | Day 30 | Day 45 | Day 60 | Day 75 | Day 90 |
| Example 1 | Golden Yellow | Golden Yellow | Golden Yellow | Yellow colour | Yellow colour | Dark yellow |
| Example 2 | Golden Yellow | Golden Yellow | Golden Yellow | Golden Yellow | Yellow colour | Dark yellow |
| Example 3 | Golden Yellow | Golden Yellow | Golden Yellow | Yellow colour | Yellow colour | Dark yellow |
| Comparative example 1 | Golden Yellow | Yellow colour | Yellow colour | Dark yellow | Black and yellow | Black and yellow |
| Comparative example 2 | Golden Yellow | Golden Yellow | Yellow colour | Dark yellow | Black and yellow | Black and yellow |
| Comparative example 3 | Golden Yellow | Yellow colour | Dark yellow | Dark yellow | Black and yellow | Black and yellow |
| Comparative example 4 | Golden Yellow | Golden Yellow | Yellow colour | Dark yellow | Black and yellow | Black and yellow |
TABLE 2 evaluation of hardness in fresh-keeping storage of Cordyceps sinensis
| Group of | Day 15 | Day 30 | Day 45 | Day 60 | Day 75 | Day 90 |
| Example 1 | Hard compaction | Hard compaction | Hard compaction | Slightly soft | Slightly soft | Softer and softer |
| Example 2 | Hard compaction | Hard compaction | Hard compaction | Hard compaction | Slightly soft | Slightly soft |
| Example 3 | Hard compaction | Hard compaction | Hard compaction | Hard compaction | Slightly soft | Softer and softer |
| Comparative example 1 | Hard compaction | Slightly soft | Slightly soft | Softer and softer | Is very soft | Is very soft |
| Comparative example 2 | Hard compaction | Slightly soft | Slightly soft | Slightly soft | Is very soft | Is very soft |
| Comparative example 3 | Hard compaction | Slightly soft | Slightly soft | Is very soft | Is very soft | Is very soft |
| Comparative example 4 | Hard compaction | Softer and softer | Is very soft | Is very soft | Is very soft | Is very soft |
TABLE 3 evaluation of Water discharge during fresh-keeping storage of Cordyceps sinensis
| Group of | Day 15 | Day 30 | Day 45 | Day 60 | Day 75 | Day 90 |
| Example 1 | No obvious water outlet | No obvious water outlet | Slightly yielding water | Slightly yielding water | Slightly yielding water | Proper water outlet |
| Example 2 | No obvious water outlet | No obvious water outlet | Slightly yielding water | Slightly yielding water | Slightly yielding water | Proper water outlet |
| Example 3 | No obvious water outlet | No obvious water outlet | Slightly yielding water | Slightly yielding water | Slightly yielding water | Proper water outlet |
| Comparative example 1 | Slightly yielding water | Proper water outlet | Proper water outlet | The water is more discharged | The water is more discharged | The water is more discharged |
| ComparisonExample 2 | Slightly yielding water | Slightly yielding water | Proper water outlet | The water is more discharged | The water is more discharged | The water is more discharged |
| Comparative example 3 | Slightly yielding water | Slightly yielding water | Slightly yielding water | Proper water outlet | The water is more discharged | The water is more discharged |
| Comparative example 4 | Slightly yielding water | Slightly yielding water | Proper water outlet | Proper water outlet | The water is more discharged | The water is more discharged |
Experimental example 2
Internal index scoring in Cordyceps sinensis fresh-keeping process
Referring to the method in experimental example 1, the weight loss rate, the soluble protein content reduction rate, the polyphenol oxidase activity increase rate of each group of Cordyceps sinensis were measured after 0 day, 45 days and 70 days relative to the weight loss rate before the fresh-keeping treatment, and the influence degree of the inside enzyme activity of Cordyceps sinensis was analyzed, and the results are shown in fig. 1 to 3.
The weight loss rate of the sample is measured by a weighing method, the weight of the fruits before and after storage is weighed, and the weight loss rate is calculated according to the following formula:
weight loss = (weight of fruit before storage-weight of fruit during storage)/weight of fruit before storage x 100%;
the method for measuring the content of the soluble protein comprises the steps of sucking 1.0mL of sample supernatant, placing the supernatant into a test tube, adding 5.0mL of coomassie brilliant blue G-250 solution, fully mixing, standing for 5min, colorizing at the wavelength of 595nm, and measuring absorbance according to the same method for manufacturing a standard curve. The method is repeated 3 times, and the content of the soluble protein is the ratio percentage of the mass/[ mu ] g of the protein to the total volume/mL of the sample extracting solution, which is found from a standard curve, to 1000 times of the product of the volume/mL of the sample extracting solution and the mass/g of the sample.
The soluble protein content decrease rate was a percentage of the ratio of the difference between the soluble protein content at 45 days and 75 days and the soluble protein content at 0 day to the soluble protein content at 0 day.
The increase rate of the polyphenol oxidase activity is measured according to the method of Jiankang, et al, physiological and biochemical experiment guidance after fruit and vegetable harvest [ M ], beijing: chinese light industry Press, 2007:59-154.
As shown in FIG. 1, the method for processing the cordyceps sinensis fresh-keeping lock nutrition by cascade treatment can reduce the weight loss rate, and the weight loss rate of comparative examples 1-4 is reduced to a larger extent.
In addition, the soluble protein participates in the regulation and control of various physiological and biochemical metabolic processes of the edible fungi, is one of important indexes of the quality and nutrition of the edible fungi, and the content reduction of the soluble protein is considered as an important characteristic of aging of the sampled samples, as shown in fig. 2, the reduction of the soluble protein can be inhibited after the treatment of the natural preservation composition in the embodiment 1-the embodiment 3, so that the higher preservation and storage quality can be maintained, and the content reduction rate of the soluble protein in the comparative example 1 is obviously higher, so that the treatment of the natural preservation composition has an important effect.
As shown in FIG. 3, the increase of polyphenol oxidase activity can be reduced in examples 1-3, the loss of Cordyceps nutrient substances during storage is reduced, the refrigerating and fresh-keeping effects on Cordyceps sinensis are improved, and the obvious effects of comparative examples 1-4 are worse.
Although embodiments of the present application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the application, the scope of which is defined in the appended claims and their equivalents.
The application and its embodiments have been described above with no limitation, and the actual construction is not limited to the embodiments of the application as shown in the drawings. In summary, if one of ordinary skill in the art is informed by this disclosure, a structural manner and an embodiment similar to the technical solution should not be creatively devised without departing from the gist of the present application.
Claims (9)
1. A method for processing the nutrition of a combined-grade processed cordyceps sinensis fresh-keeping lock is characterized by comprising the following steps: the method comprises the steps of cleaning, preserving pretreatment, stabilizing, modified atmosphere packaging and sterilization, wherein the cleaning is to clean and remove impurities attached to the surface of fresh cordyceps sinensis by using water, the preserving pretreatment is to soak cordyceps sinensis in a natural preserving composition, take out and dry the cordyceps sinensis to obtain soaked cordyceps sinensis, the stabilizing is to place the soaked cordyceps sinensis in a nitrogen closed container with the temperature of 0-4 ℃ for 5-8 hours, the modified atmosphere packaging is to fill the stabilized cordyceps sinensis into packaging materials, and the packaging gas is filled for modified atmosphere packaging; sterilizing with ultraviolet light for 1-2 hr to obtain sterilized Cordyceps product, and preserving at 0-4deg.C;
the natural fresh-keeping composition comprises the following components in parts by weight: 10-15 parts of echinacea purpurea root extract, 5-10 parts of goose phyllostachys pubescens leaf extract, 1-5 parts of secoisolariciresinol diglucoside, 0.5-3 parts of vanillic acid and the balance of water.
2. The method for processing the cordyceps sinensis fresh-keeping lock nutrition by cascade treatment according to claim 1, which is characterized by comprising the following steps of: the ultrasonic cleaning is carried out for many times at low frequency, the power of the ultrasonic cleaning is 550-1200W, the frequency of the ultrasonic cleaning is 35-60kHz, the times of the ultrasonic cleaning are 5-8 times, and the time of the ultrasonic cleaning is 3-5 minutes per time.
3. The method for processing the cordyceps sinensis fresh-keeping lock nutrition by cascade treatment according to claim 2, which is characterized by comprising the following steps of: the fresh-keeping pretreatment time is 3-5 hours, the temperature of the fresh-keeping pretreatment is 0-4 ℃, stirring is continuously carried out in the fresh-keeping pretreatment process, and the stirring frequency is 5-10 revolutions per minute.
4. The method for processing the cordyceps sinensis fresh-keeping lock nutrition by cascade treatment according to claim 3, which is characterized by comprising the following steps of: the natural fresh-keeping composition comprises the following components in parts by weight: 12-15 parts of echinacea purpurea root extract, 8-10 parts of goose phyllostachys pubescens leaf extract, 1-3 parts of secoisolariciresinol diglucoside, 0.5-2 parts of vanillic acid and the balance of water.
5. The method for processing the cordyceps sinensis fresh-keeping lock nutrition by cascade treatment according to claim 4, which is characterized by comprising the following steps of: the packaging gas consists of 5-15% of oxygen, 2-5% of carbon dioxide and the balance of nitrogen in a volume ratio of 5-15% to 2.5 times of the volume of the cordyceps sinensis.
6. The method for processing the cordyceps sinensis fresh-keeping lock nutrition by cascade treatment according to claim 5, which is characterized by comprising the following steps of: the water vapor transmission rate of the packaging material is 60-80 g/(m) 2 * 24h) The oxygen transmission amount is 30-70m 3/(m) 2 *24h 0.1 mpa); or the packaging material has a water vapor transmission rate of 65 g/(m) 2 * 24h) The oxygen transmission amount was 40m 3/(m) 2 *24h 0.1 mpa); or the packaging material has a water vapor transmission rate of 80 g/(m) 2 * 24h) The oxygen transmission amount was 70m 3/(m) 2 *24h*0.1MPa)。
7. The method for processing the cordyceps sinensis fresh-keeping lock nutrition by cascade treatment according to claim 6, which is characterized by comprising the following steps of: the packaging material is a P30S modified atmosphere box.
8. The method for processing the cordyceps sinensis fresh-keeping lock nutrition by cascade treatment according to claim 7, which is characterized by comprising the following steps of: the preparation method of the echinacea purpurea root extract comprises the following steps: weighing 2-4g of dried echinacea purpurea root, soaking in 80% ethanol, extracting with ultrasonic wave for 3 times, mixing filtrates, concentrating under reduced pressure, and oven drying at 45deg.C; the preparation method of the goose phyllostachys pubescens leaf extract comprises the following steps: weighing She Ganpin-10 g of goose moso bamboo, soaking in 80% ethanol, extracting with ultrasonic method, repeating for 3 times, mixing filtrates, concentrating under reduced pressure, and oven drying at 45deg.C.
9. The method for processing the cordyceps sinensis fresh-keeping lock nutrition by cascade treatment according to claim 8, which is characterized by comprising the following steps of: the ultrasonic extraction frequency is 40kHz, the ultrasonic extraction time is 1.5-2 hours, and the ultrasonic extraction temperature is 55 ℃.
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| CN106727734A (en) * | 2017-01-05 | 2017-05-31 | 广东东阳光药业有限公司 | Fresh cordyceps sinensis product and preparation method thereof |
| CN106923139A (en) * | 2017-02-24 | 2017-07-07 | 广东东阳光药业有限公司 | A kind of intelligent fresh-keeping system of fresh Cordyceps sinensis |
| CN109717346A (en) * | 2019-03-08 | 2019-05-07 | 中国科学院西北高原生物研究所 | A kind of method of the fresh-keeping cordyceps sinensis of irradiation method |
| CN110122730A (en) * | 2019-06-21 | 2019-08-16 | 广东东阳光药业有限公司 | The fresh jelly liquid of one kind, its preservation method and its application |
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| CN106727734A (en) * | 2017-01-05 | 2017-05-31 | 广东东阳光药业有限公司 | Fresh cordyceps sinensis product and preparation method thereof |
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| CN110122730A (en) * | 2019-06-21 | 2019-08-16 | 广东东阳光药业有限公司 | The fresh jelly liquid of one kind, its preservation method and its application |
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