CN109699539A - A method of hybridize suitable for river snail different geographic populations - Google Patents
A method of hybridize suitable for river snail different geographic populations Download PDFInfo
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- CN109699539A CN109699539A CN201811601698.7A CN201811601698A CN109699539A CN 109699539 A CN109699539 A CN 109699539A CN 201811601698 A CN201811601698 A CN 201811601698A CN 109699539 A CN109699539 A CN 109699539A
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Abstract
It mainly include that net cage preparations, isolated rearing, standby parent select and match and hybridize the invention discloses a kind of method suitable for the hybridization of river snail different geographic populations.The river snail of different geographic populations, different sexes, different Hybrid is isolated in different net cages and is raised by this method, and according to their own growth characteristics independent control cultivation water temperature and dissolved oxygen amount in suitable range, the river snail growth for meeting different cultivars requires, different geographical river snail population hybridization can be accurately constructed in this way, step is simple, easily operated and popularization, it cultivates simultaneously and is suitble to the bottom food organisms film of dwelling of aquatic breeding field snail that can provide the bait of high nutrition for river snail and will cause the pollution of environment.
Description
Technical field
The invention belongs to aquaculture technical fields, and in particular to it is a kind of suitable for river snail different geographic populations hybridization
Method.
Background technique
River snail refers to the mollusk of Viviparidae, belongs to Mollusca Gastropoda Probranchia Viviparidae, Chinese big
Portion area is distributed.River snail is liked living in the waters that cool in summer and warm in winter, substrate is soft, bait is abundant, water quality is pure and fresh, especially
Like the place for being clustered in miniflow water.River snail feeding habits are miscellaneous, main to absorb water plant young stem and leaf, organic debris etc., and river snail likes
It joyous nocturnalism and ingests, growth optimal temperature is 20~27 DEG C.The nutritive value of river snail is very high, and every hectogram spiral shell meat contains albumen
18.2 grams of matter, 0.6 gram of fat also contains carbohydrate and calcium, phosphorus, iron, vitamins and other nutritious components, wherein the content of calcium
It is especially high.River snail is also a kind of medicinal animal, and spiral shell is sweet in flavor, cold in nature, has heat-clearing, improving eyesight, diuresis, treating stranguria and other effects." this
Careless detailed outline " record that " river snail dampness removing heat-clearing, quenches the thirst and sober up, sharp stool and urine；Control tinea pedis, jaundice."
Recently as the rapid growth of river snail consumption market, aquatic breeding field snail base, aquatic breeding field snail field have been organized in many places.In
State's number of patent application is that the patent application of CN201711164497.0 discloses " a kind of method of artificial breeding river snail ", is passed through
Seedling selection, domesticating and cultivating, branch mailbox cultivation and the cultivation of young spiral shell and etc. provide a large amount of qualities good young spiral shell, solve crop field breeding children
The problems such as spiral shell is encroached on vulnerable to helminth, pathogenic bacteria, and the speed of growth is slow, survival rate is low；China Patent No. is
The Chinese patent of CN201410339318.2 discloses " a kind of cultural method of high mountain shell river snail ", and this method passes through in height above sea level
For null is arranged in 800-1200 meters of rice fields, and river snail is bred and cultivated in null, then harvested in second year, no
Cultivated area is rationally utilized in the usable floor area that reduced by only arable land, and has obtained preferable yield.But up to the present
Rarely have about river snail different geographic populations hybridization research report, due to different geographic populations river snail spiral case quality and meat not
It is identical to the greatest extent, respectively there is a feature, the river snail that High aititude mountain area produces mostly shell is thin intolerant to transport, and but meat is splendid, tasty mouthfeel；Low sea
Although pulling out the more thick and solid resistance to transport of river snail spiral case that Plain produces, but what mouthfeel and taste were often produced not as good as High aititude mountain area
River snail, so obtaining good river snail kind by the hybridization of river snail different geographic populations has very high researching value.
Summary of the invention
In view of the above deficiencies, the present invention provides a kind of method suitable for the hybridization of river snail different geographic populations, this method
Different geographical river snail population hybridization can be accurately constructed, step is simple, easily operated.
The present invention is achieved by the following technical scheme:
A method of hybridize suitable for river snail different geographic populations, mainly comprises the steps that
(1) net cage prepares: it is miscellaneous that rice field or pool are first divided into isolated rearing area, female spiral shell raising area, male spiral shell raising area and pairing
Area is handed over, above-mentioned each region is mutually isolated, then the plastic wire of 30~40 mesh or metal mesh is selected to be made into net cage, net cage is immersed
In the rice field or pool of isolated rearing area, female spiral shell raising area, male spiral shell raising area and pairing hybridization region, net cage open end is higher than
Then any one or two kinds of mixtures in rice straw and sugarcane toppers leaf are launched as fixation matrix and are existed by 4~6cm of the water surface
Net cage bottom, distribution density are 1 Cu/㎡, then introduce a fine variety benthic algae and mini-Frac test to fixation matrix, and according to
5kg/ mus of amount discharges biology to the rice field of isolated rearing area, female spiral shell raising area, male spiral shell raising area and pairing hybridization region or pool
Fertilizer just covers with the bottom that one layer of suitable river snail ingests and dwells food organisms film after culture 7~10 days on fixation matrix；
(2) isolated rearing: carrying out primary election to the river snails of different geographic populations, eliminates disability individual, selects and remain that spiral case is complete, health has
Vigor, the river snail that specification is 25~35g/ individual, the correspondence net being thrown to respectively by different geographic populations in isolated rearing area
It is carried out isolated rearing 1~2 month in case, net cage is distinguish with place of production Sign Board；River snail injected volume is 1kg/m2, control river snail
Temperature of cultivation is 20~25 DEG C, continues to be oxygenated into net cage using oxygen pump, guarantees that water-soluble oxygen amount is 2~3 ppm, changes within every 5~7 days
Water is primary, discharges bio-fertilizer to the rice field in isolated rearing area or pool according to 5kg/ mus of amount within every 10~15 days；
(3) standby parent selects: carrying out standby parent after step (2) isolated rearing 1~2 month and selects, according to right feeler
Curling person is male spiral shell, and the same person of two feeler shapes is that female spiral shell carries out male and female differentiation to river snail；Select specification for 35~40g/,
The female spiral shell not mated is maternal as standby, and is thrown to the correspondence net cage in female spiral shell raising area respectively according to different geographic populations
Interior progress unisexuality raising, net cage mating group and gender Sign Board are distinguish, and female spiral shell injected volume is 0.5kg/m2, control river snail and support
Growing water temperature is 20~25 DEG C, continues to be oxygenated into net cage using oxygen pump, guarantees that water-soluble oxygen amount is 2.5~3.5 ppm, every 5~7 days
It changes that water is primary, discharges bio-fertilizer to the rice field in female spiral shell raising area or pool according to 5kg/ mus of amount within every 7~8 days；Select specification for
The big specification hero spiral shell that 30~38g/ is a, testis development is perfect is launched respectively as standby male parent, and according to different geographic populations
Unisexuality raising is carried out in correspondence net cage into male spiral shell raising area, net cage mating group and gender Sign Board are distinguish, and male spiral shell is thrown
It is high-volume 0.5kg/m2, control aquatic breeding field snail water temperature is 20~25 DEG C, continues to be oxygenated into net cage using oxygen pump, guarantees water-soluble oxygen
Amount is 2~3 ppm, changes within every 5~7 days that water is primary, raises within every 10~15 days rice field or the water in area to male spiral shell according to 5kg/ mu of amount
Discharge bio-fertilizer in the pool；
(4) pairing hybridization: the female spiral shell and male spiral shell picked out in step (3) are after isolated rearing 1~2 month, by different population
The individual of gonadal maturation carries out pairing hybridization, carries out isolation feeding in the correspondence net cage being then thrown in pairing hybridization region
It supports, net cage is distinguish with cross combination Sign Board, and river snail injected volume is 1kg/m2, control aquatic breeding field snail water temperature is 20~25
DEG C, continue to be oxygenated into net cage using oxygen pump, guarantees that water-soluble oxygen amount is 2~3 ppm, it is primary to change within every 5~7 days water, and every 10~15
It discharges bio-fertilizer to the rice field of pairing hybridization region or pool according to 5kg/ mus of amount, finally obtains river snail cross combination.
Further, benthic algae as described in step (1) is any one or a few of chlorella, diatom and green alga.
Further, mini-Frac test as described in step (1) is wheel animalcule.
Further, bio-fertilizer described in step (1) is uniformly mixed by the raw material of following parts by weight: shrimp peptide ammino acid
20~30 parts of organic material, 5~10 parts of sodium humate, 5~10 parts of amino acid chelated calcium, 5~10 parts of zinc-amino acid chelate, amino acid
5~10 parts of chelated iron, 10~15 parts of turfy soil, 100~150 parts of water.
It further, is by weight to introduce a fine variety 8 parts of benthic algaes and 2 parts of mini-Frac tests to adhesive substrate in step (1)
Matter.
The river snail of above-mentioned different geographic populations is the river snail of the river snail peace original seed group of high mountain population.
The technical program has the advantages that compared with prior art
1, the present invention uses rice straw or sugarcane toppers leaf as fixation matrix and introduces a fine variety benthic algae and mini-Frac test group,
The biological organic fertilizer of science addition simultaneously promotes benthic algae and the mini-Frac test growth and breeding on fixed object, finally turns out
Be suitble to aquatic breeding field snail bottom dwell food organisms film be river snail hybridization raise the raising bait of health environment-friendly is provided.
2, the present invention uses isolated rearing method, by different geographic populations, different sexes, different Hybrid river snail every
It is raised from different net cages, and provides different growing environments for it according to the female spiral shell speed of growth different with male spiral shell,
The river snail growth for meeting different cultivars requires, and is conducive to improve aquatic breeding field snail efficiency.
3, since the molten amount of oxygen of the river snail to cultivation water is more sensitive, so continuing blowing aeration side using artificial in the present invention
Method guarantees that the molten amount of oxygen of cultivation water can satisfy the appearance requirement of river snail, while also refreshing the water periodically to keep the pure and fresh cleaning of water quality.
Specific embodiment
The present invention is further illustrated by the following examples, but not as limitation of the present invention.In the following example not
Dated specific experiment condition and method, used technological means are usually conventional hand well-known to those skilled in the art
Section.
Embodiment 1:
A method of hybridize suitable for river snail different geographic populations, mainly comprises the steps that
(1) net cage prepares: in early April, 2018, and rice field or pool are first divided into isolated rearing area, female spiral shell raising area, male spiral shell
Area and pairing hybridization region are raised, above-mentioned each region is mutually isolated, and then select the plastic wire of 35 mesh or metal mesh to be made into net cage,
Net cage is immersed in rice field or pool, net cage open end is higher than water surface 5cm, then throws rice straw as fixation matrix
It is placed on net cage bottom, distribution density is 1 Cu/㎡, then by weight introduces a fine variety 8 parts of benthic algaes and 2 parts of mini-Frac tests
To fixation matrix, and according to 5kg/ mus of amount to isolated rearing area, female spiral shell raising area, male spiral shell raising area and the rice for matching hybridization region
Bio-fertilizer is discharged in field or pool, after culture 10 days, the bottom that one layer of suitable river snail ingests just is covered on fixation matrix and is dwelt food organisms
Film；The benthic algae is chlorella, diatom and green alga；The mini-Frac test is wheel animalcule；The bio-fertilizer by
The raw material of following parts by weight is uniformly mixed: 25 parts of shrimp peptide ammino acid organic material, 7 parts of sodium humate, amino acid chelated calcium 7
Part, 6 parts of zinc-amino acid chelate, 7 parts of amino acid chelated iron, 14 parts of turfy soil, 110 parts of water；
(2) isolated rearing: to Yuanbaoshan Power Station population river snail (high mountain population river snail) and Quanzhou population river snail (Plain population river snail) into
Disability individual is eliminated in row primary election, the river snail individual that spiral case is complete, health is vibrant, specification is 30g/ is selected and remain, by different geography
Population is thrown in the correspondence net cage in isolated rearing area respectively and carries out isolated rearing 2 months, and net cage is subject to place of production Sign Board
It distinguishes；River snail injected volume is 1kg/m2, control aquatic breeding field snail water temperature is 20~25 DEG C, continues to be oxygenated into net cage using oxygen pump,
Guarantee that water-soluble oxygen amount is 2 ppm, changes within every 5 days that water is primary, the every 10 days rice fields or water according to 5kg/ mus of amount to isolated rearing area
Discharge bio-fertilizer in the pool；
(3) standby parent selects: carrying out standby parent after step (2) isolated rearing 2 months and selects, crimps according to right feeler
Person is male spiral shell, and the same person of two feeler shapes is that female spiral shell carries out male and female differentiation to river snail；It selects specification for 38g/, mate
Female spiral shell carries out single as standby female parent, and in the correspondence net cage being thrown in female spiral shell raising area respectively according to different geographic populations
Property raising, net cage mating group and gender Sign Board be distinguish, and female spiral shell injected volume is 0.5kg/m2, controlling aquatic breeding field snail water temperature is
20~25 DEG C, continue to be oxygenated into net cage using oxygen pump, guarantee that water-soluble oxygen amount is 3 ppm, it is primary to change within every 5 days water, every 7 days according to
5kg/ mus of amount discharges bio-fertilizer to the rice field in female spiral shell raising area or pool；Select specification perfect for 30g/, testis development
Big specification hero spiral shell is thrown in the correspondence net cage in male spiral shell raising area respectively as standby male parent, and according to different geographic populations
Unisexuality raising is carried out, net cage mating group and gender Sign Board are distinguish, and male spiral shell injected volume is 0.5kg/m2, control aquatic breeding field snail
Water temperature is 20~25 DEG C, continues to be oxygenated into net cage using oxygen pump, guarantees that water-soluble oxygen amount is 2 ppm, and it is primary to change within every 5 days water, often
Bio-fertilizer is discharged to the rice field in male spiral shell raising area or pool according to 5kg/ mus of amount within 10 days；
(4) pairing hybridization: the female spiral shell and male spiral shell picked out in step (3) are after isolated rearing 1 month, by the sexual gland of different population
The individual reached maturity carries out pairing hybridization, carries out isolated rearing, net in the correspondence net cage being then thrown in pairing hybridization region
Case is distinguish with cross combination Sign Board, and river snail injected volume is 1kg/m2, control aquatic breeding field snail water temperature is 20~25 DEG C, is used
Oxygen pump continues to be oxygenated into net cage, guarantees that water-soluble oxygen amount is 2.5 ppm, changes within every 5 days that water is primary, and every 10 days according to 5kg/ mus of amount
Bio-fertilizer is discharged to the rice field of pairing hybridization region or pool, finally obtains following 2 cross combination of river snail: Yuanbaoshan Power Station population river snail
(high mountain population river snail) ♀ × Quanzhou population river snail (Plain population river snail) ♂ and Quanzhou population river snail (Plain population river snail) ♀ ×
Yuanbaoshan Power Station population river snail (high mountain population river snail) ♂.
Embodiment 2:
A method of hybridize suitable for river snail different geographic populations, mainly comprises the steps that
(1) net cage prepares: in mid-April, 2018, and rice field or pool are first divided into isolated rearing area, female spiral shell raising area, male spiral shell
Area and pairing hybridization region are raised, above-mentioned each region is mutually isolated, and then select the plastic wire of 40 mesh or metal mesh to be made into net cage,
Net cage is immersed in rice field or pool, net cage open end is higher than water surface 6cm, then throws sugarcane toppers leaf as fixation matrix
It is placed on net cage bottom, distribution density is 1 Cu/㎡, then by weight introduces a fine variety 8 parts of benthic algaes and 2 parts of mini-Frac tests
To fixation matrix, and according to 5kg/ mus of amount to isolated rearing area, female spiral shell raising area, male spiral shell raising area and the rice for matching hybridization region
Bio-fertilizer is discharged in field or pool, after culture 7 days, the bottom that one layer of suitable river snail ingests just is covered on fixation matrix and is dwelt food organisms
Film；The benthic algae is diatom and green alga；The mini-Frac test is wheel animalcule；The bio-fertilizer is by following weight
The raw material of number is uniformly mixed: 30 parts of shrimp peptide ammino acid organic material, 5 parts of sodium humate, 10 parts of amino acid chelated calcium, amino
10 parts of sour chelated zinc, 10 parts of amino acid chelated iron, 10 parts of turfy soil, 100 parts of water；
(2) isolated rearing: to Yuanbaoshan Power Station population river snail (high mountain population river snail) and Hubei population river snail (Plain population river snail) into
Disability individual is eliminated in row primary election, the river snail individual that spiral case is complete, health is vibrant, specification is 25g/ is selected and remain, by different geography
Population is thrown in the correspondence net cage in isolated rearing area respectively and carries out isolated rearing 1 month, and net cage is subject to place of production Sign Board
It distinguishes；River snail injected volume is 1kg/m2, control aquatic breeding field snail water temperature is 20~25 DEG C, continues to be oxygenated into net cage using oxygen pump,
Guarantee that water-soluble oxygen amount is 2.5 ppm, change within every 7 days that water is primary, every 15 days according to 5kg/ mus rice field from amount to isolated rearing area or
Discharge bio-fertilizer in pool；
(3) standby parent selects: carrying out standby parent after step (2) isolated rearing 1 month and selects, crimps according to right feeler
Person is male spiral shell, and the same person of two feeler shapes is that female spiral shell carries out male and female differentiation to river snail；It selects specification for 40g/, mate
Female spiral shell carries out single as standby female parent, and in the correspondence net cage being thrown in female spiral shell raising area respectively according to different geographic populations
Property raising, net cage mating group and gender Sign Board be distinguish, and female spiral shell injected volume is 0.5kg/m2, controlling aquatic breeding field snail water temperature is
20~25 DEG C, continue to be oxygenated into net cage using oxygen pump, guarantee that water-soluble oxygen amount is 2.5 ppm, it is primary to change within every 6 days water, presses within every 8 days
Bio-fertilizer is discharged to the rice field in female spiral shell raising area or pool according to 5kg/ mus of amounts；Select specification perfect for 34g/, testis development
Big specification hero spiral shell as standby male parent, and the correspondence net cage being thrown to respectively according to different geographic populations in male spiral shell raising area
Interior progress unisexuality raising, net cage mating group and gender Sign Board are distinguish, and male spiral shell injected volume is 0.5kg/m2, control river snail and support
Growing water temperature is 20~25 DEG C, continues to be oxygenated into net cage using oxygen pump, guarantees that water-soluble oxygen amount is 3 ppm, and it is primary to change within every 5 days water,
Bio-fertilizer is discharged to the rice field in male spiral shell raising area or pool according to 5kg/ mus of amount within every 10 days；
(4) pairing hybridization: the female spiral shell and male spiral shell picked out in step (3) are after isolated rearing 2 months, by the sexual gland of different population
The individual reached maturity carries out pairing hybridization, carries out isolated rearing, net in the correspondence net cage being then thrown in pairing hybridization region
Case is distinguish with cross combination Sign Board, and river snail injected volume is 1kg/m2, control aquatic breeding field snail water temperature is 20~25 DEG C, is used
Oxygen pump continues to be oxygenated into net cage, guarantees that water-soluble oxygen amount is 2 ppm, changes within every 7 days that water is primary, every 15 days according to 5kg/ mus amount to
Bio-fertilizer is discharged in the rice field or pool for matching hybridization region, and finally obtain following 2 cross combination of river snail: Hubei population river snail is (flat
Original seed group river snail) ♀ × Yuanbaoshan Power Station population river snail (high mountain population river snail) ♂ and Yuanbaoshan Power Station population river snail (high mountain population river snail) ♀
× Hubei population river snail (Plain population river snail) ♂.
Embodiment 3:
A method of hybridize suitable for river snail different geographic populations, mainly comprises the steps that
(1) net cage prepares: in late April, 2018, and rice field or pool are first divided into isolated rearing area, female spiral shell raising area, male spiral shell
Area and pairing hybridization region are raised, above-mentioned each region is mutually isolated, and then select the plastic wire of 30 mesh or metal mesh to be made into net cage,
Net cage is immersed in rice field or pool, net cage open end is higher than water surface 4cm, then by the mixed of rice straw and sugarcane toppers leaf
It closes object to launch as fixation matrix in net cage bottom, distribution density is 1 Cu/㎡, then by weight by 8 parts of benthic algaes and 2
Part mini-Frac test is introduced a fine variety to fixation matrix, and is raised according to 5kg/ mus of amount to isolated rearing area, female spiral shell raising area, male spiral shell
The rice field or pool of area and pairing hybridization region discharge bio-fertilizer and just cover with one layer of suitable river snail on fixation matrix after culture 9 days and take the photograph
The bottom of food is dwelt food organisms film；The benthic algae is green alga；The mini-Frac test is wheel animalcule；The bio-fertilizer
It is uniformly mixed by the raw material of following parts by weight: 20 parts of shrimp peptide ammino acid organic material, 10 parts of sodium humate, chelating amino acids
5 parts of calcium, 5 parts of zinc-amino acid chelate, 5 parts of amino acid chelated iron, 15 parts of turfy soil, 150 parts of water；
(2) isolated rearing: to Yuanbaoshan Power Station population river snail (high mountain population river snail) and Liujiang population river snail (Plain population river snail) into
Disability individual is eliminated in row primary election, the river snail individual that spiral case is complete, health is vibrant, specification is 35g/ is selected and remain, by different geography
Population is thrown in the correspondence net cage in isolated rearing area respectively and carries out isolated rearing 1 month, and net cage is subject to place of production Sign Board
It distinguishes；River snail injected volume is 1kg/m2, control aquatic breeding field snail water temperature is 20~25 DEG C, continues to be oxygenated into net cage using oxygen pump,
Guarantee that water-soluble oxygen amount is 3 ppm, changes within every 6 days that water is primary, the every 13 days rice fields or water according to 5kg/ mus of amount to isolated rearing area
Discharge bio-fertilizer in the pool；
(3) standby parent selects: carrying out standby parent after step (2) isolated rearing 1 month and selects, crimps according to right feeler
Person is male spiral shell, and the same person of two feeler shapes is that female spiral shell carries out male and female differentiation to river snail；It selects specification for 35g/, mate
Female spiral shell carries out single as standby female parent, and in the correspondence net cage being thrown in female spiral shell raising area respectively according to different geographic populations
Property raising, net cage mating group and gender Sign Board be distinguish, and female spiral shell injected volume is 0.5kg/m2, controlling aquatic breeding field snail water temperature is
20~25 DEG C, continue to be oxygenated into net cage using oxygen pump, guarantee that water-soluble oxygen amount is 3.5 ppm, it is primary to change within every 7 days water, presses within every 7 days
Bio-fertilizer is discharged to the rice field in female spiral shell raising area or pool according to 5kg/ mus of amounts；Select specification perfect for 38g/, testis development
Big specification hero spiral shell as standby male parent, and the correspondence net cage being thrown to respectively according to different geographic populations in male spiral shell raising area
Interior progress unisexuality raising, net cage mating group and gender Sign Board are distinguish, and male spiral shell injected volume is 0.5kg/m2, control river snail and support
Growing water temperature is 20~25 DEG C, continues to be oxygenated into net cage using oxygen pump, guarantees that water-soluble oxygen amount is 2.5 ppm, changes water one within every 6 days
It is secondary, bio-fertilizer is discharged to the rice field in male spiral shell raising area or pool according to 5kg/ mus of amount within every 12 days；
(4) pairing hybridization: the female spiral shell and male spiral shell picked out in step (3) are after isolated rearing 2 months, by the sexual gland of different population
The individual reached maturity carries out pairing hybridization, carries out isolated rearing, net in the correspondence net cage being then thrown in pairing hybridization region
Case is distinguish with cross combination Sign Board, and river snail injected volume is 1kg/m2, control aquatic breeding field snail water temperature is 20~25 DEG C, is used
Oxygen pump continues to be oxygenated into net cage, guarantees that water-soluble oxygen amount is 3 ppm, changes within every 6 days that water is primary, every 12 days according to 5kg/ mus amount to
Bio-fertilizer is discharged in the rice field or pool for matching hybridization region, finally obtains following 2 cross combination of river snail: Yuanbaoshan Power Station population river snail
(high mountain population river snail) ♀ × Liujiang population river snail (Plain population river snail) ♂ and Liujiang population river snail (Plain population river snail) ♀ ×
Yuanbaoshan Power Station population river snail (high mountain population river snail) ♂.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (5)
1. a kind of method suitable for the hybridization of river snail different geographic populations, it is characterised in that: it is mainly comprised the steps that
(1) net cage prepares: it is miscellaneous that rice field or pool are first divided into isolated rearing area, female spiral shell raising area, male spiral shell raising area and pairing
Area is handed over, above-mentioned each region is mutually isolated, then the plastic wire of 30~40 mesh or metal mesh is selected to be made into net cage, net cage is immersed
In the rice field or pool of isolated rearing area, female spiral shell raising area, male spiral shell raising area and pairing hybridization region, net cage open end is higher than
Then any one or two kinds of mixtures in rice straw and sugarcane toppers leaf are launched as fixation matrix and are existed by 4~6cm of the water surface
Net cage bottom, distribution density are 1 Cu/㎡, then introduce a fine variety benthic algae and mini-Frac test to fixation matrix, and according to
5kg/ mus of amount discharges biology to the rice field of isolated rearing area, female spiral shell raising area, male spiral shell raising area and pairing hybridization region or pool
Fertilizer just covers with the bottom that one layer of suitable river snail ingests and dwells food organisms film after culture 7~10 days on fixation matrix；
(2) isolated rearing: carrying out primary election to the river snails of different geographic populations, eliminates disability individual, selects and remain that spiral case is complete, health has
Vigor, the river snail that specification is 25~35g/ individual, the correspondence net being thrown to respectively by different geographic populations in isolated rearing area
It is carried out isolated rearing 1~2 month in case, net cage is distinguish with place of production Sign Board；River snail injected volume is 1kg/m2, control river snail
Temperature of cultivation is 20~25 DEG C, continues to be oxygenated into net cage using oxygen pump, guarantees that water-soluble oxygen amount is 2~3 ppm, changes within every 5~7 days
Water is primary, discharges bio-fertilizer to the rice field in isolated rearing area or pool according to 5kg/ mus of amount within every 10~15 days；
(3) standby parent selects: carrying out standby parent after step (2) isolated rearing 1~2 month and selects, according to right feeler
Curling person is male spiral shell, and the same person of two feeler shapes is that female spiral shell carries out male and female differentiation to river snail；Select specification for 35~40g/,
The female spiral shell not mated is maternal as standby, and is thrown to the correspondence net cage in female spiral shell raising area respectively according to different geographic populations
Interior progress unisexuality raising, net cage mating group and gender Sign Board are distinguish, and female spiral shell injected volume is 0.5kg/m2, control river snail and support
Growing water temperature is 20~25 DEG C, continues to be oxygenated into net cage using oxygen pump, guarantees that water-soluble oxygen amount is 2.5~3.5 ppm, every 5~7 days
It changes that water is primary, discharges bio-fertilizer to the rice field in female spiral shell raising area or pool according to 5kg/ mus of amount within every 7~8 days；Select specification for
The big specification hero spiral shell that 30~38g/ is a, testis development is perfect is launched respectively as standby male parent, and according to different geographic populations
Unisexuality raising is carried out in correspondence net cage into male spiral shell raising area, net cage mating group and gender Sign Board are distinguish, and male spiral shell is thrown
It is high-volume 0.5kg/m2, control aquatic breeding field snail water temperature is 20~25 DEG C, continues to be oxygenated into net cage using oxygen pump, guarantees water-soluble oxygen
Amount is 2~3 ppm, changes within every 5~7 days that water is primary, raises within every 10~15 days rice field or the water in area to male spiral shell according to 5kg/ mu of amount
Discharge bio-fertilizer in the pool；
(4) pairing hybridization: the female spiral shell and male spiral shell picked out in step (3) are after isolated rearing 1~2 month, by different population
The individual of gonadal maturation carries out pairing hybridization, carries out isolation feeding in the correspondence net cage being then thrown in pairing hybridization region
It supports, net cage is distinguish with cross combination Sign Board, and river snail injected volume is 1kg/m2, control aquatic breeding field snail water temperature is 20~25
DEG C, continue to be oxygenated into net cage using oxygen pump, guarantees that water-soluble oxygen amount is 2~3 ppm, it is primary to change within every 5~7 days water, and every 10~15
It discharges bio-fertilizer to the rice field of pairing hybridization region or pool according to 5kg/ mus of amount, finally obtains river snail cross combination.
2. a kind of method suitable for the hybridization of river snail different geographic populations according to claim 1, it is characterised in that: step
(1) benthic algae described in is any one or a few of chlorella, diatom and green alga.
3. a kind of method suitable for the hybridization of river snail different geographic populations according to claim 1, it is characterised in that: step
(1) mini-Frac test described in is wheel animalcule.
4. a kind of method suitable for the hybridization of river snail different geographic populations according to claim 1, it is characterised in that: step
(1) bio-fertilizer described in is uniformly mixed by the raw material of following parts by weight: 20~30 parts of shrimp peptide ammino acid organic material, humic
5~10 parts of sour sodium, 5~10 parts of amino acid chelated calcium, 5~10 parts of zinc-amino acid chelate, 5~10 parts of amino acid chelated iron, turf
10~15 parts, 100~150 parts of water of soil.
5. a kind of method suitable for the hybridization of river snail different geographic populations according to claim 1, it is characterised in that: step
(1) 8 parts of benthic algaes and 2 parts of mini-Frac tests are introduced a fine variety to fixation matrix by weight in.
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