CN109662927B

CN109662927B - Whitening and freckle removing cream and preparation method thereof

Info

Publication number: CN109662927B
Application number: CN201910095382.3A
Authority: CN
Inventors: 王丽辉; 季小洋; 林锦雄
Original assignee: Guangzhou Aibei Biological Technology Co Ltd
Current assignee: Guangzhou Aibei Biological Technology Co Ltd
Priority date: 2019-01-31
Filing date: 2019-01-31
Publication date: 2021-11-23
Anticipated expiration: 2039-01-31
Also published as: CN109662927A

Abstract

The invention relates to a whitening and freckle-removing cream and a preparation method thereof, belonging to the field of cosmetics. The whitening and freckle-removing cream comprises the following freckle-removing agents in percentage by weight: 0.05-3% of undecylenoyl phenylalanine, 0.01-10% of 3-o-ethyl ascorbic acid, 0.01-5% of a mixture a, 0.01-5% of a mixture b and 0.01-5% of a mixture c; also comprises the following skin conditioning agents in percentage by weight: 0.01-1% of bisabolol, 0.01-5% of phytosterol oleate, 0.01-5% of silanediol salicylate and 0.01-1% of sodium hyaluronate; the mixture a comprises phenethyl resorcinol; the mixture b comprises yeast extract; said mixture c comprises hydrolysed plums. The whitening and freckle-removing cream disclosed by the invention is safe, free of side effects, capable of inhibiting inflammation and good in whitening and freckle-removing effects.

Description

Whitening and freckle removing cream and preparation method thereof

Technical Field

The invention relates to a whitening and freckle-removing cream and a preparation method thereof, belonging to the field of cosmetics.

Background

Skin whitening is a permanent topic and pursuit of vast women who love beauty. The development, safety and efficacy evaluation of whitening cosmetics naturally become a focus of attention of researchers, production operators and consumers. The color of the skin is mainly determined by the pigment content and distribution of the skin, and melanin is the most important determinant. The content of melanin in skin determines the skin color, the synthesis of melanin is a biological reaction process, wherein the activity of a rate-limiting enzyme tyrosinase plays a decisive role, and meanwhile, melanin granules are transferred to keratinocytes through dendritic projections of the melanocytes, and the melanin granules transferred to the keratinocytes move upwards to the stratum corneum along with epidermal cells, so that the color of the skin is influenced or color spots are formed, and finally, the melanin granules are discharged along with the shedding of the stratum corneum.

The whitening and freckle-removing cream is an emulsified cream for skin, which has certain inhibiting, dissolving and removing effects on skin tarnish, uneven skin color and spots on the surface of the skin. At present, the general skin whitening and freckle removing products mainly achieve the effects of whitening and removing freckles by inhibiting tyrosinase activity or inhibiting the transfer of melanosomes, thereby reducing the production of melanin and fading the existing freckles. The skin whitening and freckle removing product mainly comprises arbutin, hydroquinone and derivatives thereof, L-ascorbic acid and derivatives thereof, kojic acid and derivatives thereof, vitamin C ethyl ether and derivatives thereof, nicotinamide, tranexamic acid, benzenediol and derivatives thereof and the like. However, the skin whitening and freckle removing product cannot comprehensively consider all links of skin whitening and freckle removing, has single function and effect, is not ideal, and can still bring the risk of anaphylactic infection after being used. Therefore, the research and development of a whitening and freckle-removing product which is safe, has no side effect, has good whitening and freckle-removing effects and can inhibit inflammation is particularly necessary.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide the whitening and freckle-removing cream which is safe, has no side effect, has good whitening and freckle-removing effects and can inhibit inflammation and the preparation method thereof.

In order to achieve the purpose, the invention adopts the technical scheme that: a whitening and freckle-removing cream comprises the following freckle-removing agents in percentage by weight: 0.05-3% of undecylenoyl phenylalanine, 0.01-10% of 3-o-ethyl ascorbic acid, 0.01-5% of a mixture a, 0.01-5% of a mixture b and 0.01-5% of a mixture c; also comprises the following skin conditioning agents in percentage by weight: 0.01-1% of bisabolol, 0.01-5% of phytosterol oleate, 0.01-5% of silanediol salicylate and 0.01-1% of sodium hyaluronate; the mixture a comprises phenethyl resorcinol; the mixture b comprises yeast extract; said mixture c comprises hydrolysed plums.

Preferably, the undecylenoyl phenylalanine is sold under the name of SEPIWHITE MSH, has a structure similar to that of melanocyte stimulating hormone (a-MSH), can control the combination of the a-MSH and melanin generation factors, further blocks the melanin formation process, comprehensively inhibits the melanin generation from multiple links, and has obvious, durable, safe and reliable improvement effect on skin color spots.

Preferably, the 3-o-ethyl ascorbic acid is VC ethyl ether, which can effectively inhibit the activity of tyrosinase, prevent the formation of melanin, and achieve the effects of improving color spots and whitening skin.

Preferably, said mixture a, having the trade name Multisal ® White, comprising hydrolyzed corn starch, phenylethyl resorcinol, hydrated silica; the microsphere is attached to the skin, active substances are slowly released along with the time, the slow release greatly reduces the stimulation to the skin, and the process of the skin absorbing the phenethyl resorcinol is very mild.

Preferably, the mixture b is commercially available as DermalrX SRC, which comprises water, yeast extract, soybean amino acids, phenoxyethanol; said mixture c, with the trade name Clairju (hydrolyzed cherry plum), Chinese name hydrolyzed European plum (PRUNUS DOMESTICA), comprises butylene glycol, water, hydrolyzed European plum; the yeast extract and the hydrolyzed European plums have synergistic effect, and can effectively promote the renewal of horny layer and keratinocyte to achieve the effects of whitening and removing freckles.

Preferably, the silanediol salicylate is under the trade name of DSBC, the phytosterol oleate is under the trade name of phytosterol ester, and the bisabolol is under the trade name of bisabolol, the silanediol salicylate, the phytosterol ester and the bisabolol, which can effectively inhibit inflammation generation, and the combined use of the three can achieve better effect of synergistically inhibiting inflammation generation.

As a preferred embodiment of the whitening and freckle-removing cream, the whitening and freckle-removing cream further comprises the following emollients in percentage by weight: 0.1-5% of hydrogenated polyisobutene, 0.1-10% of isononyl isononanoate, 0.01-10% of camellia seed oil, 0.1-5% of polydimethylsiloxane, 0.1-5% of cyclopenta dimethyl siloxane, 0.1-10% of shea butter and 0.1-10% of cocoa seed fat.

As a preferred embodiment of the whitening and freckle-removing cream, the whitening and freckle-removing cream further comprises the following emulsifiers in percentage by weight: 0.1-5% of mixture d, 0.1-5% of mixture e and 0.01-3% of potassium cetyl phosphate.

As a preferred embodiment of the whitening and freckle-removing cream, the whitening and freckle-removing cream further comprises the following thickening agents in percentage by weight: 0.01-3% of sodium acrylate copolymer/lecithin, 0.01-1% of carbomer, 0.1-5% of cetearyl alcohol and 0.1-3% of mixture f.

As a preferred embodiment of the whitening and freckle-removing cream, the whitening and freckle-removing cream further comprises the following preservatives in percentage by weight: 0.01-0.4% of methyl hydroxybenzoate, 0.01-0.1% of propyl hydroxybenzoate, 0.01-1% of phenoxyethanol/ethylhexyl glycerol and 0.01-0.3% of sodium bisulfite.

As a preferred embodiment of the whitening and freckle-removing cream, the whitening and freckle-removing cream further comprises the following antioxidants in percentage by weight: 0.01-0.1% of pentaerythritol tetra (bis-tert-butyl hydroxy hydrocinnamate) and 0.01-3% of tocopherol.

According to a preferred embodiment of the whitening and freckle-removing cream, the whitening and freckle-removing cream further comprises, by weight, 0.01-1% of a pH regulator, 0.1-10% of a humectant, 0.01-1% of an aromatic and a solvent, wherein the solvent is water, the pH regulator is aminomethyl propanol, the humectant is butanediol, the aromatic is essence.

As a preferred embodiment of the invention, the whitening and freckle-removing cream comprises the following components in percentage by weight: 1% of undecylenoyl phenylalanine, 3-o-ethyl ascorbic acid, 3% of the mixture a 3%, 2% of the mixture b, 3% of the mixture c, 0.03% of bisabolol, 1% of phytosterol oleate, 1% of silanediol salicylate, 0.03% of sodium hyaluronate, 3% of hydrogenated polyisobutene, 2% of isononyl isononanoate, 2% of camellia seed oil, 2% of polydimethylsiloxane, 2% of cyclopentyldimethylsiloxane, 2% of shea butter, 3% of cocoa seed butter, 2% of the mixture d, 2% of the mixture e, 0.3% of potassium cetyl phosphate, 0.5% of sodium/lecithin (acrylate) copolymer, 0.15% of carbomer, 3% of cetostearyl alcohol, 0.5% of the mixture f, 0.2% of methyl hydroxybenzoate, 0.1% of propylhydroxybenzoate, 0.5% of phenoxyethanol/ethylhexylglycerol, 0.1% of sodium bisulfite, 0.1% of, Pentaerythritol tetra (di-tert-butyl hydroxy hydrocinnamic acid) ester 0.3%, tocopherol 0.3%, aminomethyl propanol 0.07%, butanediol 1%, essence 0.1%, and water to 100%.

Further, the mixture d comprises one or more of arachidyl alcohol, behenyl alcohol, arachidyl alcohol glucoside, water and glucose; the mixture d is sold under the trade name MONTANOV 202.

Further, the mixture e comprises one or more of glyceryl stearate, cetearyl alcohol, stearic acid, sodium lauroyl glutamate; the mixture e is known under the trade name emulsifier HP.

Further, the mixture f comprises one or more of sodium acrylate, acryloyl dimethyl sodium taurate copolymer, isohexadecane, polysorbate-80, water and sorbitan oleate; the mixture f is sold under the name SIMULGEL EG.

The invention also provides a preparation method of the whitening and freckle-removing cream, which comprises the following steps:

(1) weighing the following raw materials in proportion:

phase A raw materials: water, butanediol, sodium hyaluronate, sodium acrylate copolymer/lecithin, and carbomer;

b phase raw materials: mixture d, mixture e, hydrogenated polyisobutene, isononyl isononanoate, camellia oil, cetostearyl alcohol, polydimethylsiloxane, cyclopentadimethylsiloxane, undecylenoylphenylalanine, methylparaben, propylparaben, pentaerythritol tetrakis (di-tert-butyl hydroxyhydrocinnamate), bisabolol, phytosterol oleate, tocopherol, shea butter, cocoa butter, potassium cetyl phosphate, mixture a;

c phase raw material: a mixture f;

d phase raw material: aminomethyl propanol;

e phase raw material: 3-o-ethyl ascorbic acid, sodium bisulfite, mixture b, mixture c, silanediol salicylate, phenoxyethanol/ethylhexyl glycerol, and essence;

(2) sequentially adding the phase A raw materials in the step (1) into a water phase pot, heating to 85-90 ℃, fully and uniformly stirring until the phase A raw materials are completely dissolved, and keeping the temperature for later use;

(3) sequentially adding the raw materials of the phase B in the step (1) into an oil phase pot, heating to 80-85 ℃, fully and uniformly stirring, and keeping the temperature for later use;

(4) pumping the water phase pot material obtained in the step (2) into a vacuum emulsification stirring pot, stirring at a constant speed until the water phase pot material is uniform, pumping the oil phase pot material obtained in the step (3) into the stirring pot, stirring uniformly, homogenizing and emulsifying for 5-8 minutes, adding the phase C, homogenizing for 2-3 minutes, fully stirring uniformly again, and keeping the temperature for 30 minutes;

(5) cooling the components obtained in the step (4) to 60 ℃, sequentially adding the D-phase raw materials, and uniformly stirring for later use;

(6) cooling the components obtained in the step (5) to 45 ℃, sequentially adding the E-phase raw materials, and fully and uniformly stirring for later use;

(7) and (4) cooling the components obtained in the step (6) to 38 ℃, discharging, and standing to obtain the whitening and freckle-removing cream.

Compared with the prior art, the invention has the beneficial effects that:

(1) the whitening and freckle-removing cream contains undecylenoyl phenylalanine, can control the combination of a-MSH and melanin generation factors, further blocks the process of melanin formation, comprehensively inhibits the melanin generation from multiple links, and has obvious, lasting, safe and reliable improvement effect on skin color spots;

(2) the whitening and freckle-removing cream contains 3-o-ethyl ascorbic acid, and can effectively inhibit the activity of tyrosinase, prevent the formation of melanin, and achieve the effects of improving color spots and whitening skin;

(3) the combination of the undecylenoyl phenylalanine and the 3-o-ethyl ascorbic acid has a synergistic effect, can better inhibit the generation of melanin, and has better whitening and spot-lightening effects;

(4) the whitening and freckle-removing cream contains phenethyl resorcinol, can effectively reduce pigmentation by injecting microspheres into skin cells to achieve the whitening effect, and the microspheres are attached to the skin to slowly release active substances, so that the stimulation to the skin is greatly reduced, the application range of the whitening and freckle-removing cream is expanded, and the whitening and freckle-removing cream can be used by the face, the body and sensitive people;

(5) the whitening spot-fading cream contains yeast extract and hydrolyzed European plums, and the two have synergistic effect, so that the renewal of cuticle and keratinocytes can be effectively promoted, and the whitening spot-fading effect can be achieved;

(6) the whitening and spot-fading cream contains silanediol salicylate, phytosterol ester and bisabolol, all of which can effectively inhibit inflammation, and the effect of synergistically inhibiting inflammation is better when the three are used in combination;

in a word, the whitening and freckle-removing cream disclosed by the invention is safe, has no side effect, can inhibit inflammation and has a good whitening and freckle-removing effect.

Detailed Description

To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.

Example 1

A whitening and freckle-removing cream comprises the following components in percentage by weight: 1% of undecylenoyl phenylalanine, 3-o-ethyl ascorbic acid, 3% of the mixture a 3%, 2% of the mixture b, 3% of the mixture c, 0.03% of bisabolol, 1% of phytosterol oleate, 1% of silanediol salicylate, 0.03% of sodium hyaluronate, 3% of hydrogenated polyisobutene, 2% of isononyl isononanoate, 2% of camellia seed oil, 2% of polydimethylsiloxane, 2% of cyclopentyldimethylsiloxane, 2% of shea butter, 3% of cocoa seed butter, 2% of the mixture d, 2% of the mixture e, 0.3% of potassium cetyl phosphate, 0.5% of sodium/lecithin (acrylate) copolymer, 0.15% of carbomer, 3% of cetearyl alcohol, 0.5% of the mixture f, 0.2% of methyl hydroxybenzoate, 0.1% of propylhydroxybenzoate, 0.5% of phenoxyethanol/ethylhexylglycerol, 0.1% of sodium bisulfite, pentaerythritol tetra (di-tert-butyl hydroxy hydrocinnamic acid) ester 0.3%, tocopherol 0.3%, aminomethyl propanol 0.07%, butanediol 1%, essence 0.1%, and water to 100%; the mixture a comprises hydrolyzed corn starch, phenethyl resorcinol, hydrated silica; the mixture b comprises water, yeast extract, soybean amino acid and phenoxyethanol; the mixture c comprises butanediol, water and hydrolyzed plums; the mixture d comprises arachidyl alcohol, behenyl alcohol, arachidyl alcohol glucoside, water and glucose; the mixture e comprises glyceryl stearate, cetearyl alcohol, stearic acid, sodium lauroyl glutamate; the mixture f comprises sodium acrylate, acryloyl dimethyl taurate copolymer, isohexadecane, polysorbate-80, water and sorbitan oleate.

The preparation method of the whitening and freckle-removing cream comprises the following steps:

(1) weighing the following raw materials in proportion:

c phase raw material: a mixture f;

d phase raw material: aminomethyl propanol;

(2) sequentially adding the phase A raw materials in the step (1) into a water phase pot, heating to 85 ℃, fully and uniformly stirring until the phase A raw materials are completely dissolved, and keeping the temperature for later use;

(3) sequentially adding the raw materials of the phase B in the step (1) into an oil phase pot, heating to 80 ℃, fully and uniformly stirring, and keeping the temperature for later use;

(4) pumping the water phase pot material obtained in the step (2) into a vacuum emulsification stirring pot, stirring at a constant speed until the mixture is uniform, pumping the oil phase pot material obtained in the step (3) into the stirring pot, stirring uniformly, homogenizing and emulsifying for 5 minutes, adding the phase C, homogenizing for 2 minutes, fully stirring uniformly again, and keeping the temperature for 30 minutes;

Example 2

A whitening and freckle-removing cream is different from example 1 in the following listed components, and the other components are the same as example 1: 1% of undecylenoyl phenylalanine, 3% of 3-o-ethyl ascorbic acid, 3% of phenethyl resorcinol, 2% of yeast extract, 3% of hydrolyzed European plums, 0.03% of bisabolol, 1% of phytosterol oleate, 1% of silanediol salicylate and 0.03% of sodium hyaluronate.

(1) weighing the following raw materials in proportion:

c phase raw material: a mixture f;

d phase raw material: aminomethyl propanol;

(2) sequentially adding the phase A raw materials in the step (1) into a water phase pot, heating to 90 ℃, fully and uniformly stirring until the phase A raw materials are completely dissolved, and keeping the temperature for later use;

(3) sequentially adding the raw materials of the phase B in the step (1) into an oil phase pot, heating to 85 ℃, fully and uniformly stirring, and keeping the temperature for later use;

(4) pumping the water phase pot material obtained in the step (2) into a vacuum emulsification stirring pot, stirring at a constant speed until the mixture is uniform, pumping the oil phase pot material obtained in the step (3) into the stirring pot, stirring uniformly, homogenizing and emulsifying for 8 minutes, adding the phase C, homogenizing for 3 minutes, fully stirring uniformly again, and keeping the temperature for 30 minutes;

Example 3

A whitening and freckle-removing cream is different from example 1 in the following listed components, and the other components are the same as example 1: 0.05% of undecylenoyl phenylalanine, 0.01% of 3-o-ethyl ascorbic acid, 0.01% of phenethyl resorcinol, 0.01% of yeast extract, 0.01% of hydrolyzed European plum, 0.01% of bisabolol, 0.01% of phytosterol oleate, 0.01% of silanediol salicylate and 0.01% of sodium hyaluronate.

(1) weighing the following raw materials in proportion:

c phase raw material: a mixture f;

d phase raw material: aminomethyl propanol;

(2) sequentially adding the phase A raw materials in the step (1) into a water phase pot, heating to 88 ℃, fully and uniformly stirring until the phase A raw materials are completely dissolved, and keeping the temperature for later use;

(3) sequentially adding the raw materials of the phase B in the step (1) into an oil phase pot, heating to 83 ℃, fully and uniformly stirring, and keeping the temperature for later use;

(4) pumping the water phase pot material obtained in the step (2) into a vacuum emulsification stirring pot, stirring at a constant speed until the mixture is uniform, pumping the oil phase pot material obtained in the step (3) into the stirring pot, stirring uniformly, homogenizing and emulsifying for 6 minutes, adding the phase C, homogenizing for 3 minutes, fully stirring uniformly again, and keeping the temperature for 30 minutes;

Example 4

A whitening and freckle removing cream is different from example 1 in the following listed components, and other components and preparation methods are the same as example 1: hydrogenated polyisobutene 0.1%, isononyl isononanoate 0.1%, camellia seed oil 0.01%, polydimethylsiloxane 0.1%, cyclopenta dimethyl siloxane 0.1%, shea butter 0.1%, cocoa seed butter 0.1%.

Example 5

A whitening and freckle removing cream is different from example 1 in the following listed components, and other components and preparation methods are the same as example 1: 5% of hydrogenated polyisobutene, 10% of isononyl isononanoate, 10% of camellia seed oil, 5% of polydimethylsiloxane, 5% of cyclopentadimethylsiloxane, 10% of shea butter and 0.1-10% of cocoa butter.

Example 6

A whitening and freckle removing cream is different from example 1 in the following listed components, and other components and preparation methods are the same as example 1: mixture d 0.1%, mixture e 0.1%, potassium cetyl phosphate 0.01%;

example 7

A whitening and freckle removing cream is different from example 1 in the following listed components, and other components and preparation methods are the same as example 1: mixture d 5%, mixture e 5%, potassium cetyl phosphate 3%.

Example 8

A whitening and freckle removing cream is different from example 1 in the following listed components, and other components and preparation methods are the same as example 1: 0.01% of sodium acrylate copolymer/lecithin, 0.01% of carbomer, 0.1% of cetostearyl alcohol and 0.1% of mixture f.

Example 9

A whitening and freckle removing cream is different from example 1 in the following listed components, and other components and preparation methods are the same as example 1: 3% of sodium acrylate copolymer/lecithin, 1% of carbomer, 5% of cetostearyl alcohol and 3% of mixture f.

Example 10

A whitening and freckle removing cream is different from example 1 in the following listed components, and other components and preparation methods are the same as example 1: 0.01% of methyl hydroxybenzoate, 0.01% of propyl hydroxybenzoate, 0.01% of phenoxyethanol/ethylhexyl glycerol and 0.01% of sodium bisulfite.

Example 11

A whitening and freckle removing cream is different from example 1 in the following listed components, and other components and preparation methods are the same as example 1: pentaerythritol tetra (di-tert-butyl hydroxyhydrocinnamic acid) ester 0.01%, tocopherol 0.01%.

Example 12

A whitening and freckle removing cream is different from example 1 in the following listed components, and other components and preparation methods are the same as example 1: pentaerythritol tetra (bis-tert-butyl hydroxyhydrocinnamic acid) ester 0.1% and tocopherol 3%.

Example 13

A whitening and freckle removing cream is different from example 1 in the following listed components, and other components and preparation methods are the same as example 1: 0.01% of aminomethyl propanol, 0.1% of butanediol and 0.01% of essence.

Example 14

A whitening and freckle removing cream is different from example 1 in the following listed components, and other components and preparation methods are the same as example 1: 1% of aminomethyl propanol, 10% of butanediol and 1% of essence.

Comparative example 1

A whitening and freckle-removing cream is prepared by the same method as in example 1 except that the cream does not contain undecylenoyl phenylalanine.

Comparative example 2

A whitening and freckle-removing cream comprises the following components and a preparation method which are the same as those in example 1 except that 3-O-ethyl ascorbic acid is not contained:

comparative example 3

A whitening and freckle-removing cream is prepared by the same method as the embodiment 1 except that phenethyl resorcinol is not contained.

Comparative example 4

A whitening and spot-removing cream has the same components and preparation method as in example 1 except that it does not contain yeast extract and hydrolyzed European plums.

Comparative example 5

A whitening and freckle-removing cream is prepared by the same method as in example 1 except that the cream does not contain the bisabolol, the phytosterol oleate and the silanediol salicylate.

Product evaluation

Firstly, evaluating the safety of products

The whitening and freckle-removing creams of examples 1 to 14 and comparative examples 1 to 5 were evaluated for irritation of cosmetics.

The test population: 100 women aged 20-50 years have healthy skin and no allergic history of skin diseases, and accord with the voluntary selection standard of the subjects.

Grouping: the test population was divided into 20 groups of 5 individuals each, with the members in the group differing in age by no more than 2 years.

The spot pasting method comprises the following steps: selecting a qualified spot tester, placing 0.020-0.025g of a tested object in the spot tester in a closed spot patch experimental mode, attaching the spot tester with the tested object to the bent side of the back or the forearm of a tested object by using a non-irritating adhesive tape, slightly pressing the bent side with a palm to uniformly attach the tested object to the skin, removing the tested object after 24 hours, observing skin reactions after 0.5, 6, 12, 24 and 48 hours after the tested object is removed, and recording the results according to skin reaction grading standards in cosmetic hygiene standards and cosmetic safety technical standards, wherein the test results are shown in table 1.

Evaluation criteria: level 0: negative reaction; level 1: suspicious reactions, only faint erythema; and 2, stage: weak positive reaction, erythema, infiltration, edema, and possibly pimple; and 3, level: strong positive reaction, erythema, infiltration, edema, pimple and reaction beyond the tested area; 4, level: very strong positive reaction, obvious erythema, severe infiltration, edema, herpes fusiformis and reaction beyond the tested area.

Table 1: safety detection of whitening and freckle removing cream for each group

Test product	Patch test 0.5h (negative number of people)	Patch test 6h (negative number of people)	Patch test 12h (negative number of people)	Patch test 24h (negative number of people)	Patch test 48h (negative number of people)
						Example 1	5	5	5	5	5
Example 2	5	5	5	5	5
						Example 3	5	5	5	5	5
Example 4	5	5	5	5	5
						Example 5	5	5	5	5	5
Example 6	5	5	5	5	5
						Example 7	5	5	5	5	5
Example 8	5	5	5	5	5
						Example 9	5	5	5	5	5
Example 10	5	5	5	5	5
						Example 11	5	5	5	5	5
Example 12	5	5	5	5	5
						Example 13	5	5	5	5	5
Example 14	5	5	5	5	5
						Comparative example 1	5	5	5	5	5
Comparative example 2	5	5	5	5	5
						Comparative example 3	5	5	5	5	5
Comparative example 4	5	5	5	5	5
						Comparative example 5	5	5	5	5	5

As can be seen from Table 1, the results of the human skin patch test show that the skin reactions of all groups of test subjects are observed within 0.5 hour, 6 hours, 12 hours, 24 hours and 48 hours after all groups of test subjects pass the patch test of all groups of test substances, and the skin reactions of all the test subjects are negative, so that the whitening and freckle-removing cream prepared by the method is safe to use.

Second, product functionality evaluation

Efficacy tests were performed on the whitening and spot-removing creams of examples 1 to 14 and comparative examples 1 to 5.

(1) Test for Melanin reduction Rate

Selecting 100 women and testing the skin melanin reduction rate between the ages of 20-50, dividing a tested human group into 20 groups, respectively correspondingly coating the whitening and freckle-removing creams of examples 1-14 and comparative examples 1-5 on 5 persons in each group and 20 groups of subjects, taking stratum corneum cells of the skin of the subjects by using a scraping blade before the subjects are coated with the whitening and freckle-removing creams for the first time, storing for later use, coating the whitening and freckle-removing creams for 28 days, taking stratum corneum cells of the same subjects at the same position, respectively dyeing the stratum corneum cells scraped in the two times, respectively, visualizing the melanin content, and performing data analysis on skin images of the subjects to obtain a melanin semi-quantitative analysis result, wherein the test result is shown in a table 2.

(2) Inhibition of tyrosinase assay

Respectively taking 0.025g of the whitening spot-lightening creams of the examples 1 to 14 and the comparative examples 1 to 5, placing the whitening spot-lightening creams into test tubes, wherein the numbers are 1 to 20, respectively adding 120 mu L of phosphate buffer solution and 40 mu L of 2.98 mmol/L of tyrosine solution (2.98 mmol/L of tyrosine solution is used as a substrate) into the test tubes 1 to 20, respectively adding the mixed solution in the test tubes 1 to 20 in equal amount into a 96-well plate, adding 40 mu L of 200U/mL of tyrosinase solution into each well after the constant temperature of water bath at 37 ℃ is kept for 10 min, uniformly mixing the mixture under the condition of keeping out of the sun at room temperature, quickly placing the mixture in a multifunctional microplate detector after the water bath at 37 ℃ is carried out for 15 min, measuring the absorbance value at 475 nm, calculating the tyrosinase inhibition rate, repeating the operation, carrying out parallel measurement for 3 times, and obtaining the test results shown in Table 2.

(3) Determination of DPPH radical scavenging Activity

Antioxidant activity is expressed as the ability to scavenge DPPH free radicals. According to the experimental conditions determined by the preliminary experiment, the activities of the whitening and freckle-removing cream of the examples 1 to 14 and the comparative examples 1 to 5 for removing the DPPH (free fatty radical) are measured. Taking 1.0g of each sample, adding 2.0 ml of 1.52X 10-4mmol/L DPPH absolute ethanol solution, mixing well, standing at room temperature in dark place for 30min, taking a solvent as a blank control, and measuring the absorbance A1 at 517 nm; replacing the sample solution with the same volume of solvent to serve as a negative control, and measuring the absorbance A2; the absorbance A3 was measured using the same volume of absolute ethanol as the blank instead of the DPPH solution. VC was used as a control, 3 replicates of each group were averaged, the test results are shown in table 2, and the clearance was calculated as follows:

the clearance (%) of the sample to DPPH was [1- (a1-A3)/a2] × 100%.

TABLE 2 whitening and speckle-removing efficacy test of each group of whitening and speckle-removing cream

Group of	Melanin reduction ratio (%)	Tyrosinase inhibition (%)	Oxygen radical DPPH clearance (%)
				Example 1	65	55	35
Example 2	61	54	34
				Example 3	52	52	33
Example 4	60	58	30
				Example 5	60	52	31
Example 6	63	51	28
				Example 7	64	51	29
Example 8	63	55	30
				Example 9	65	55	30
Example 10	60	58	31
				Example 11	59	54	32
Example 12	58	55	34
				Example 13	55	56	33
Example 14	59	54	30
				Comparative example 1	58	47	30
Comparative example 2	49	49	31
				Comparative example 3	48	40	25
Comparative example 4	45	46	25
				Comparative example 5	55	43	26

From the test results in table 2, it can be seen that the melanin reduction rate of the subjects coated with the whitening and freckle-removing creams of examples 1 to 14 is lower than that of comparative examples 1 and 5 only in example 3, and example 14 is equivalent to comparative example 5 but lower than that of comparative example 1, and the other examples are higher than that of comparative example; the subjects who are smeared with the whitening and freckle-removing cream in the examples 1 to 14 have higher tyrosinase inhibition rate than the subjects who are smeared with the whitening and freckle-removing cream in the comparative examples 1 to 5; the DPPH clearance rate of oxygen free radicals of the subjects coated with the whitening and freckle-removing cream of the examples 1 to 3 is higher than that of the comparative examples 1 to 5, and the DPPH clearance rates of the examples 4 to 14 are equivalent to that of the comparative examples 1 and 2 but are higher than that of the comparative examples 3 to 5; therefore, the whitening and freckle-removing cream provided by the invention can effectively reduce melanin, inhibit the activity of tyrosinase, has strong antioxidant activity and has good whitening and freckle-removing effects. The whitening and freckle-removing cream in example 1 has the best efficacy, and the melanin reduction rate, the tyrosinase inhibition rate and the oxygen radical DPPH clearance rate are the highest, and are 65%, 55% and 35% respectively.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. The whitening and freckle-removing cream is characterized by comprising the following freckle-removing agents in percentage by weight: 0.05-3% of undecylenoyl phenylalanine, 0.01-10% of 3-o-ethyl ascorbic acid, 0.01-5% of a mixture a, 0.01-5% of a mixture b and 0.01-5% of a mixture c; the mixture a comprises phenethyl resorcinol; the mixture b comprises yeast extract; said mixture c comprises hydrolysed plums;

also comprises the following skin conditioning agents in percentage by weight: 0.01-1% of bisabolol, 0.01-5% of phytosterol oleate, 0.01-5% of silanediol salicylate and 0.01-1% of sodium hyaluronate; the preservative also comprises the following preservatives in percentage by weight: 0.01-0.4% of methyl hydroxybenzoate, 0.01-0.1% of propyl hydroxybenzoate, 0.01-1% of phenoxyethanol/ethylhexyl glycerol and 0.01-0.3% of sodium bisulfite; also comprises the following emollients in percentage by weight: 0.1-5% of hydrogenated polyisobutene, 0.1-10% of isononyl isononanoate, 0.01-10% of camellia seed oil, 0.1-5% of polydimethylsiloxane, 0.1-5% of cyclopenta dimethyl siloxane, 0.1-10% of shea butter and 0.1-10% of cocoa seed fat; the emulsifier also comprises the following components in percentage by weight: 0.1-5% of mixture d, 0.1-5% of mixture e and 0.01-3% of potassium cetyl phosphate; the mixture d comprises one or more of arachidyl alcohol, behenyl alcohol, arachidyl alcohol glucoside, water and glucose; the mixture e comprises one or more of glyceryl stearate, cetearyl alcohol, stearic acid and sodium lauroyl glutamate; the thickener also comprises the following components in percentage by weight: 0.01-3% of sodium acrylate copolymer/lecithin, 0.01-1% of carbomer, 0.1-5% of cetearyl alcohol and 0.1-3% of mixture f; the mixture f comprises one or more of sodium acrylate/sodium acryloyldimethyl taurate copolymer, isohexadecane, polysorbate-80, water and sorbitan oleate; the antioxidant also comprises the following antioxidants in percentage by weight: 0.01-0.1% of pentaerythritol tetra (bis-tert-butyl hydroxy hydrocinnamate) and 0.01-3% of tocopherol; the environment-friendly type water-based deodorant also comprises 0.01-1% of a pH regulator, 0.1-10% of a humectant, 0.01-1% of an aromatic and a solvent, wherein the solvent is water, the pH regulator is aminomethyl propanol, the humectant is butanediol, the aromatic is essence.

2. The cream of claim 1, wherein the undecylenoyl phenylalanine is 1%, the 3-o-ethyl ascorbic acid is 3%, the mixture a is 3%, the mixture b is 2%, the mixture c is 3%, the bisabolol is 0.03%, the phytosterol oleate is 1%, the silanediol salicylate is 1%, and the sodium hyaluronate is 0.03% by weight of the cream.

3. The method for preparing the whitening and freckle-removing cream according to claim 1, which comprises the following steps:

(1) weighing the following raw materials in proportion:

c phase raw material: a mixture f;

d phase raw material: aminomethyl propanol;