CN109662189A - Biological fermentation feed and preparation method thereof based on needle mushroom mushroom bran - Google Patents
Biological fermentation feed and preparation method thereof based on needle mushroom mushroom bran Download PDFInfo
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- CN109662189A CN109662189A CN201910127456.7A CN201910127456A CN109662189A CN 109662189 A CN109662189 A CN 109662189A CN 201910127456 A CN201910127456 A CN 201910127456A CN 109662189 A CN109662189 A CN 109662189A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
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- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
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Abstract
The invention discloses a kind of biological fermentation feed and preparation method thereof based on needle mushroom mushroom bran, the biological fermentation feed based on needle mushroom mushroom bran, raw material including following mass fraction: 90-120 parts of needle mushroom mushroom bran, 70-90 parts of ensiling, 30-50 parts of brewex's grains, 30-50 parts of soybean residue, 20-30 parts of corn stigma 20-30, enoki mushroom roots, 20-30 parts of straw, 5-10 parts of puer tea slag, 3-5 parts of haw thorn granule and 3-5 parts of microbial bacterial agent.The present invention has many advantages, such as that digestion thoroughly, can efficiently be digested and absorbed by domestic animal, can be widely applied to biological fermentation feed technical field.
Description
Technical field
The present invention relates to biological fermentation feed technical fields.It is more particularly related to which a kind of be based on golden mushroom
Biological fermentation feed of chaff and preparation method thereof.
Background technique
Needle mushroom mushroom bran is to carry out golden mushroom plantation using raw materials such as corncob, rice bran, wheat brans, and the culture medium after harvesting is surplus
Excess, it is the residuum of Flammulina velutipes mycelium and is digested through mycelia, the compound of the ingredients such as the crude fibre of qualitative change occurs for structure.
Make animal feed, organic fertilizer using needle mushroom mushroom bran exploitation crops accessory substance, is the need developed a circular economy
It wants.Using agricultural crop straw development " planting industry --- edible fungus culturing --- aquaculture --- planting industry " Mode of Circular,
It has broad application prospects.
It is present feed research hotspot that needle mushroom mushroom bran, which is applied to field of fodder, but how to make full use of needle mushroom
The nutritive value of mushroom bran is a problem.
Summary of the invention
The object of the present invention is to provide it is a kind of digestion thoroughly, can efficiently be digested and absorbed by domestic animal based on needle mushroom
Biological fermentation feed of mushroom bran and preparation method thereof.
In order to realize these purposes and other advantages according to the present invention, a kind of biology based on needle mushroom mushroom bran is provided
Fermented feed, the raw material including following mass fraction:
90-120 parts of needle mushroom mushroom bran, 70-90 parts of ensiling, 30-50 parts of brewex's grains, 30-50 parts of soybean residue, corn stigma 20-
30, enoki mushroom roots 20-30 parts, 20-30 parts of straw, 5-10 parts of puer tea slag and 3-5 parts of microbial bacterial agent.
A kind of preparation method of the biological fermentation feed based on needle mushroom mushroom bran, comprising the following steps:
S1, by the corn stigma and enoki mushroom roots of above-mentioned mass fraction, mix and mince processing obtain the section shape of 0.5-1cm
Mixture 1, mixture 1 is added into 30-50 part of boiling water, boils 5-10min, is dried to obtain mixture after standing 2-3h
2；
Mixture 2 is carried out grinding and minces processing by S2, and the mixture 3 for obtaining the section shape of 0.3-0.5cm is spare；
S3 takes the needle mushroom mushroom bran of above-mentioned mass fraction to be pre-processed, obtains mixture 4；
S4, by the ensiling of the obtained mixture 4 of step S3 and above-mentioned mass fraction, brewex's grains, soybean residue, straw, Pu'er
Tea grounds is uniformly mixed and obtains mixture 5, and the sterile water of 1.3-1.5 times of its volume is added to mixture 5, after boiling 20-30min,
It is cooled to 35-40 DEG C, the microbial bacterial agent that above-mentioned mass fraction is added carries out anaerobic fermentation, and fermentation temperature is 35-40 DEG C, fermentation
Time is 12-16h, obtains tunning；
Step S4, is obtained that the mixture 3 obtained with step S2 is dried in tunning and haw thorn granule is mixed by S5
After conjunction, it is placed in and is granulated to obtain finished feed in feed granulator.
Preferably, step S3 the following steps are included:
C1 after taking the needle mushroom mushroom bran of the mass fraction to be crushed, crosses 3 mesh screens, obtains granular needle mushroom
Mushroom bran；
C2 soaks in water 12-24h into the granular needle mushroom mushroom bran that step C1 is obtained, the mass fraction of water is added
It is 3-4 times of mass fraction of needle mushroom mushroom bran, the needle mushroom mushroom bran of abrasive grains shape, obtains slimy mixture after immersion
6；
Cellulase, enzyme amount 38U/g, under conditions of 50-60 DEG C is added into the mixture 5 that step C2 is obtained in C3
Enzymolysis time 2h obtains mixture 7；
C4, the microbial bacterial agent for being 1 by the mixture 7 addition mass fraction, 35-37 DEG C of fermentation temperature, fermentation time
After 6-8h, mixed liquor 8 is obtained；
The mixed liquor 8 that step C4 is obtained is dried C5, obtains the mixture 4.
Preferably, the preparation process of the ensiling is as follows:
Fresh mass fraction is 40-50 parts of stalk by B1, is cut into the segment of 0.5-1cm, 4 DEG C of freezing 6-8h,
4-6 DEG C at room temperature, the liquid nitrogen of the mass fractions such as addition, and is ground into graininess rapidly, obtains stalk particle；
B2, the sterile water of 10-15 parts, 20 DEG C is added into stalk particle, and is uniformly mixed, and adds 10-15 parts, 40 DEG C
Sterile water, and be uniformly mixed obtain ensiling mixed liquor, by the ensiling mixed liquor stir 2-4h after, continue to grind, obtain blueness
Store slurries；
The lactic acid bacteria powder that mass fraction is 3-5 parts is added into ensiling slurries, stirs evenly, sealing container, in 32- by B3
It ferments 3-5 days at 39 DEG C, collects fermentation material to get ensiling.
Preferably, the stalk be corn stover, it is wheat stalk, rice straw, soybean stalk, any in broomcorn straw
It is a kind of.
Preferably, the partial size of the haw thorn granule is 1-2cm.
The present invention is include at least the following beneficial effects:
1, the mixture 3 for the section shape that corn stigma and enoki mushroom roots are prepared into is fiber substance, and is with hawthorn
Graininess after being eaten by domestic animal, strengthens the wriggling of the stomach of domestic animal, promotes stomach and assimilates ability to feed.
2, the ingredient of needle mushroom mushroom bran is more complex, is digested to it by cellulase before this, allows in needle mushroom mushroom bran
By the cellulose of gastrointestinal disturbances absorption and it cannot be degraded to the small-molecule substance that can be absorbed by gastrointestinal disturbances, then again with micro- life
Object microbial inoculum cannot be disappeared by the substance of cellulose degraded by microbial bacterial agent to other in needle mushroom mushroom bran
Change, so that needle mushroom mushroom bran to be prepared to the mixture 4 not absorbed easily.
3, ensiling have passed through greatly remained from fresh ensiling preparation, freeze grinding processing the nutrition of ensiling at
Point, and ensiling is prepared into the fermentation that have passed through lactic acid bacteria after ensiling slurries, and nutriment is refined, and is conducive to domestic animal to blueness
The digestion and absorption of storage also accelerates the body weight increase of domestic animal.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
It should be noted that experimental method described in following embodiments is unless otherwise specified conventional method, institute
Reagent and material are stated, unless otherwise specified, is commercially obtained.
Embodiment one
The preparation biological fermentation feed based on needle mushroom mushroom bran, the specific steps are as follows:
Upper 20 parts of corn stigma and 20 parts of enoki mushroom roots are mixed and mince processing and obtain the section shape of 0.5cm by S1
Mixture 1 is added into 30 parts of boiling water mixture 1, boils 5min, is dried to obtain mixture 2 after standing 2h；
Mixture 2 is carried out grinding and minces processing by S2, and the mixture 3 for obtaining the section shape of 0.3cm is spare；
S3 takes 90 parts of needle mushroom mushroom bran to be pre-processed, obtains mixture 4；
S4, by the obtained mixture 4 of step S3 and 70 parts of ensiling, 30 parts of brewex's grains, 30 parts of soybean residue, 20 parts
Straw, 5 parts of puer tea slag be uniformly mixed obtain mixture 5, to mixture 5 be added 1.3 times of its volume sterile water, boil
After 20min, 35 DEG C are cooled to, 3 parts of microbial bacterial agent is added and carries out anaerobic fermentation, fermentation temperature is 35 DEG C, and fermentation time is
12h obtains tunning；
Step S4 is obtained tunning and the mixture 3 obtained with step S2 and 3 parts of hawthorn is dried by S5
After particle mixing, it is placed in and is granulated to obtain finished feed in feed granulator.
Wherein, step S3 the following steps are included:
C1 after taking 90 parts of needle mushroom mushroom bran to be crushed, crosses 3 mesh screens, obtains granular needle mushroom mushroom bran；
C2 soaks in water 12h into the granular needle mushroom mushroom bran that step C1 is obtained, and the mass fraction of water is added as gold
3 times of mass fraction of needle mushroom chaff, the needle mushroom mushroom bran of abrasive grains shape after immersion obtains slimy mixture 6；
Cellulase, enzyme amount 38U/g, the enzyme under conditions of 50 DEG C is added into the mixture 5 that step C2 is obtained in C3
Time 2h is solved, mixture 7 is obtained；
C4, the microbial bacterial agent for being 1 by the mixture 7 addition mass fraction, 35 DEG C of fermentation temperature, fermentation time 6h
Afterwards, mixed liquor 8 is obtained；
The mixed liquor 8 that step C4 is obtained is dried C5, obtains the mixture 4.
Wherein, the preparation process of the ensiling is as follows:
B1, the stalk for being 40 parts by fresh mass fraction, is cut into the segment of 0.5cm, 4 DEG C of freezing 6h, 4 DEG C of room
Under temperature, the liquid nitrogen of the mass fractions such as addition, and it is ground into graininess rapidly, obtain stalk particle；
10 parts, 20 DEG C of sterile water are added into stalk particle, and is uniformly mixed by B2, add 10 parts, 40 DEG C sterile
Water, and be uniformly mixed and obtain ensiling mixed liquor, after the ensiling mixed liquor is stirred 2h, continues to grind, obtain ensiling slurries；
The lactic acid bacteria powder that mass fraction is 3 parts is added into ensiling slurries, stirs evenly, sealing container, at 32 DEG C by B3
Fermentation 3 days collects fermentation material to get ensiling.
Wherein, the stalk is corn stover.
The haw thorn granule partial size is 1cm.
The microbial bacterial agent is Butyrivibrio fibrisolvens, Ruminobacter amylophilus and the yellow that mass ratio is 1:2:2
Ruminobacter.
Embodiment two
The preparation biological fermentation feed based on needle mushroom mushroom bran, the specific steps are as follows:
S1, by upper 25 parts of corn stigma and 25 parts of enoki mushroom roots, mix and mince processing obtain the section shape of 0.75cm
Mixture 1, mixture 1 is added into 40 parts of boiling water, boils 7.5min, is dried to obtain mixture 2 after standing 2.5h；
Mixture 2 is carried out grinding and minces processing by S2, and the mixture 3 for obtaining the section shape of 0.4cm is spare；
S3 takes 105 parts of needle mushroom mushroom bran to be pre-processed, obtains mixture 4；
S4, by the obtained mixture 4 of step S3 and 80 parts of ensiling, 40 parts of brewex's grains, 40 parts of soybean residue, 25 parts
Straw, 7.5 parts of puer tea slag be uniformly mixed obtain mixture 5, to mixture 5 be added 1.4 times of its volume sterile water, boil
After boiling 25min, 37.5 DEG C are cooled to, 4 parts of microbial bacterial agent is added and carries out anaerobic fermentation, fermentation temperature is 37.5 DEG C, fermentation
Time is 14h, obtains tunning；
Step S4, is obtained that the mixture 3 obtained with step S2 is dried in tunning and haw thorn granule is mixed by S5
After conjunction, it is placed in and is granulated to obtain finished feed in feed granulator.
Wherein, step S3 the following steps are included:
C1 after taking 105 parts of needle mushroom mushroom bran to be crushed, crosses 3 mesh screens, obtains granular needle mushroom mushroom bran；
C2 soaks in water 18h into the granular needle mushroom mushroom bran that step C1 is obtained, and the mass fraction of water is added as gold
3.5 times of mass fraction of needle mushroom chaff, the needle mushroom mushroom bran of abrasive grains shape after immersion obtains slimy mixture 6；
Cellulase, enzyme amount 38U/g, the enzyme under conditions of 55 DEG C is added into the mixture 5 that step C2 is obtained in C3
Time 2h is solved, mixture 7 is obtained；
C4, the microbial bacterial agent for being 1 by the mixture 7 addition mass fraction, 36 DEG C of fermentation temperature, fermentation time 7h
Afterwards, mixed liquor 8 is obtained；
The mixed liquor 8 that step C4 is obtained is dried C5, obtains the mixture 4.
Wherein, the preparation process of the ensiling is as follows:
B1, the stalk for being 45 parts by fresh mass fraction, is cut into the segment of 0.75cm, 4 DEG C of freezing 7h, at 5 DEG C
At room temperature, the liquid nitrogen of the mass fractions such as addition, and it is ground into graininess rapidly, obtain stalk particle；
12.5 parts, 20 DEG C of sterile water are added into stalk particle, and is uniformly mixed B2, add 12.5 parts, 40 DEG C
Sterile water, and be uniformly mixed and obtain ensiling mixed liquor, after the ensiling mixed liquor is stirred 3h, continue to grind, obtains ensiling slurry
Liquid；
The lactic acid bacteria powder that mass fraction is 4 parts is added into ensiling slurries, stirs evenly, sealing container, at 35.5 DEG C by B3
Lower fermentation 4 days collects fermentation material to get ensiling.
Wherein, the stalk is wheat stalk.
The haw thorn granule partial size is 1.5cm.
The microbial bacterial agent is Butyrivibrio fibrisolvens, Ruminobacter amylophilus and the yellow that mass ratio is 1:2:2
Ruminobacter.
Embodiment three
The preparation biological fermentation feed based on needle mushroom mushroom bran, the specific steps are as follows:
S1, by upper 30 parts of corn stigma and 30 parts of enoki mushroom roots, mix and mince processing obtain 1cm section shape it is mixed
Object 1 is closed, mixture 1 is added into 50 parts of boiling water, boils 10min, is dried to obtain mixture 2 after standing 3h；
Mixture 2 is carried out grinding and minces processing by S2, and the mixture 3 for obtaining the section shape of 0.5cm is spare；
S3 takes 120 parts of needle mushroom mushroom bran to be pre-processed, obtains mixture 4；
S4, by the obtained mixture 4 of step S3 and 90 parts of ensiling, 50 parts of brewex's grains, 50 parts of soybean residue, 30 parts
Straw, 10 parts of puer tea slag be uniformly mixed obtain mixture 5, to mixture 5 be added 1.5 times of its volume sterile water, boil
After boiling 30min, 40 DEG C are cooled to, 5 parts of microbial bacterial agent is added and carries out anaerobic fermentation, fermentation temperature is 40 DEG C, fermentation time
For 16h, tunning is obtained；
Step S4, is obtained that the mixture 3 obtained with step S2 is dried in tunning and haw thorn granule is mixed by S5
After conjunction, it is placed in and is granulated to obtain finished feed in feed granulator.
Wherein, step S3 the following steps are included:
C1 after taking 120 parts of needle mushroom mushroom bran to be crushed, crosses 3 mesh screens, obtains granular needle mushroom mushroom bran；
C2, soaking in water into the granular needle mushroom mushroom bran that step C1 is obtained and the mass fraction of water is added for 24 hours is gold
4 times of mass fraction of needle mushroom chaff, the needle mushroom mushroom bran of abrasive grains shape after immersion obtains slimy mixture 6；
Cellulase, enzyme amount 38U/g, the enzyme under conditions of 60 DEG C is added into the mixture 5 that step C2 is obtained in C3
Time 2h is solved, mixture 7 is obtained；
C4, the microbial bacterial agent for being 1 by the mixture 7 addition mass fraction, 37 DEG C of fermentation temperature, fermentation time 8h
Afterwards, mixed liquor 8 is obtained；
The mixed liquor 8 that step C4 is obtained is dried C5, obtains the mixture 4.
Wherein, the preparation process of the ensiling is as follows:
B1, the stalk for being 50 parts by fresh mass fraction, is cut into the segment of 1cm, 4 DEG C of freezing 8h, in 6 DEG C of room temperature
Under, the liquid nitrogen of the mass fractions such as addition, and it is ground into graininess rapidly, obtain stalk particle；
15 parts, 20 DEG C of sterile water are added into stalk particle, and is uniformly mixed by B2, add 15 parts, 40 DEG C sterile
Water, and be uniformly mixed and obtain ensiling mixed liquor, after the ensiling mixed liquor is stirred 4h, continues to grind, obtain ensiling slurries；
The lactic acid bacteria powder that mass fraction is 5 parts is added into ensiling slurries, stirs evenly, sealing container, at 39 DEG C by B3
Fermentation 5 days collects fermentation material to get ensiling.
Wherein, the stalk is rice straw.
The haw thorn granule partial size is 2m.
The microbial bacterial agent is Butyrivibrio fibrisolvens, Ruminobacter amylophilus and the yellow that mass ratio is 1:2:2
Ruminobacter.
Comparative test, specific experiment scheme are as follows:
The beef cattle that 32 weight are consistent as far as possible, are in a good state of health is selected to be randomly divided into control group by weight phase approximately principle
With test group one, test group two, test group three, every group of 8 oxen, big column is raised, and original body mass difference is not significant between each group.From
By drinking water, metering feeding, formal test 30 days, initially ox body was weighed at the end of for test, and test site is Hubei Province beef cattle
Feed lot.
Wherein, the feed that test group one, test group two, test group three are fed is respectively embodiment one, embodiment two, implements
Feed prepared by example three, and experimental group is the needle mushroom mushroom bran material of purchase, using quantitative feeding, multiple feeding is fed every time
Feed is the 0.5% of beef cattle weight.Test result is as follows:
One, 4 points of every afternoon checks the defecation of every beef cattle, and every group of eight beef cattles are checked, every group
It checks 240 times altogether, appearance is bottle green, distinguishes result such as following table.
| Group/index | First moisture (mean value) | External appearance characteristic shapes, without particle number | External appearance characteristic is shapeless, has particle number |
| Test group one | 73.5% | 205 | 35 |
| Test group two | 74.8% | 221 | 19 |
| Test group three | 76.5% | 228 | 12 |
| Control group | 80.6% | 163 | 77 |
Interpretation of result: compare between test group and control group, it is known that, eat embodiment one, embodiment two and embodiment three
The defecation situation of the beef cattle of the feed of preparation is preferable, and the beef cattle excrement sample moisture content of control group is high, inclined compared with normal cow dung sample
Dilute, excrement does not form, and has particle, also illustrates that feed digestion utilization power is poor.
Two, after 30 days, the increased average value record of four groups of beef cattle weights is as follows:
Interpretation of result: the weight gain of beef cattle after 30 days, test group obviously increase weight much than control group, therefore it can be concluded that test
Group obtains more the nutrient absorption of feed, more preferably.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and embodiment shown and described herein.
Claims (6)
1. a kind of biological fermentation feed based on needle mushroom mushroom bran, which is characterized in that the raw material including following mass fraction:
90-120 parts of needle mushroom mushroom bran, 70-90 parts of ensiling, 30-50 parts of brewex's grains, 30-50 parts of soybean residue, corn stigma 20-30, gold
20-30 parts of mushroom root of needle mushroom, 20-30 parts of straw, 5-10 parts of puer tea slag, 3-5 parts of haw thorn granule and 3-5 parts of microbial bacterial agent.
2. a kind of preparation method of the biological fermentation feed based on needle mushroom mushroom bran as described in claim 1 any one,
It is characterized in that, comprising the following steps:
S1, by the corn stigma and enoki mushroom roots of above-mentioned mass fraction, mix and mince processing obtain 0.5-1cm section shape it is mixed
Object 1 is closed, mixture 1 is added into 30-50 parts of boiling water, boils 5-10min, is dried to obtain mixture 2 after standing 2-3h；
Mixture 2 is carried out grinding and minces processing by S2, and the mixture 3 for obtaining the section shape of 0.3-0.5cm is spare；
S3 takes the needle mushroom mushroom bran of above-mentioned mass fraction to be pre-processed, obtains mixture 4；
S4, by the ensiling of the obtained mixture 4 of step S3 and above-mentioned mass fraction, brewex's grains, soybean residue, straw, puer tea slag
It is uniformly mixed and obtains mixture 5, the sterile water of 1.3-1.5 times of its volume is added to mixture 5, it is cooling after boiling 20-30min
To 35-40 DEG C, the microbial bacterial agent that above-mentioned mass fraction is added carries out anaerobic fermentation, and fermentation temperature is 35-40 DEG C, fermentation time
For 12-16h, tunning is obtained；
Step S4, is obtained that the mixture 3 obtained with step S2 is dried in tunning and haw thorn granule is mixed by S5
Afterwards, it is placed in and is granulated to obtain finished feed in feed granulator.
3. the preparation method of the biological fermentation feed as claimed in claim 2 based on needle mushroom mushroom bran, which is characterized in that step
S3 the following steps are included:
C1 after taking the needle mushroom mushroom bran of the mass fraction to be crushed, crosses 3 mesh screens, obtains granular needle mushroom mushroom bran；
C2 soaks in water 12-24h into the granular needle mushroom mushroom bran that step C1 is obtained, and the mass fraction of water is added as gold
3-4 times of mass fraction of needle mushroom chaff, the needle mushroom mushroom bran of abrasive grains shape after immersion, obtains slimy mixture 6；
C3 is added cellulase, enzyme amount 38U/g, digests under conditions of 50-60 DEG C into the mixture 5 that step C2 is obtained
Time 2h obtains mixture 7；
C4, the microbial bacterial agent for being 1 by the mixture 7 addition mass fraction, 35-37 DEG C of fermentation temperature, fermentation time 6-8h
Afterwards, mixed liquor 8 is obtained；
The mixed liquor 8 that step C4 is obtained is dried C5, obtains the mixture 4.
4. the preparation method of the biological fermentation feed as claimed in claim 2 based on needle mushroom mushroom bran, which is characterized in that described
The preparation process of ensiling is as follows:
B1, the stalk for being 40-50 parts by fresh mass fraction, is cut into the segment of 0.5-1cm, 4 DEG C of freezing 6-8h, in 4-6
DEG C at room temperature, it the liquid nitrogen of mass fractions such as is added, and is ground into graininess rapidly, obtain stalk particle；
B2, the sterile water of 10-15 parts, 20 DEG C is added into stalk particle, and is uniformly mixed, and adds the nothing of 10-15 parts, 40 DEG C
Bacterium water, and be uniformly mixed and obtain ensiling mixed liquor, after the ensiling mixed liquor is stirred 2-4h, continue to grind, obtains ensiling slurry
Liquid；
The lactic acid bacteria powder that mass fraction is 3-5 parts is added into ensiling slurries, stirs evenly, sealing container, at 32-39 DEG C by B3
Lower fermentation 3-5 days collects fermentation material to get ensiling.
5. the preparation method of the biological fermentation feed as claimed in claim 4 based on needle mushroom mushroom bran, which is characterized in that described
Stalk is corn stover, wheat stalk, rice straw, soybean stalk, any one in broomcorn straw.
6. the biological fermentation feed as described in claim 1 based on needle mushroom mushroom bran, which is characterized in that the haw thorn granule
Partial size is 1-2cm.
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