CN109588080A - A kind of high altitude localities preventing from heavy metal Alfalfa Growing method - Google Patents

A kind of high altitude localities preventing from heavy metal Alfalfa Growing method Download PDF

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CN109588080A
CN109588080A CN201811481688.4A CN201811481688A CN109588080A CN 109588080 A CN109588080 A CN 109588080A CN 201811481688 A CN201811481688 A CN 201811481688A CN 109588080 A CN109588080 A CN 109588080A
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seed
soil
alfalfa
sowing
rhizobium
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袁绪川
王世刚
杨磊
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Hunan Bo Chuan Agricultural Development Co Ltd
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Hunan Bo Chuan Agricultural Development Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G20/00—Cultivation of turf, lawn or the like; Apparatus or methods therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08—Immunising seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A01N37/04—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof polybasic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a kind of high altitude localities preventing from heavy metal Alfalfa Growing methods: sowing surface management, seed disinfection, alfalfa seed is impregnated in liquor potassic permanganate after disinfection, alfalfa seed will be sowed with base manure by then impregnating alfalfa seed in the strain soil liquid containing bacillus subtilis and rhizobium；Sowing, which is finished, to be covered seed with thin soil and treads；After sowing in 3-5 days, at the sowing near spray oxalic acid solution.Germinating capacity of the alfalfa in High aititude heavy-metal contaminated soil can be improved in implantation methods of the invention, protects high altitude localities ecological environment, promotes the development of animal husbandry.

Description

A kind of high altitude localities preventing from heavy metal Alfalfa Growing method
Technical field
The invention belongs to field of agricultural cultivation, are related to Alfalfa Growing method, in particular to raising Altitude Regions are anti-heavy Metal Alfalfa Growing method.
Background technique
China has a vast territory, and Qinghai-Tibet Platean is known as the title of " roof of the world ", and high altitude localities rich in mineral resources is China Intensively, recently as high altitude localities expanding economy, mineral resources large-scale develop and utilize important heavy metal mineral products, It discards the heavy-metal residuals such as a large amount of cadmiums, lead in the soil, causes serious heavy metal pollution.High altitude localities ecosystem resistance Low, soil is easy to be destroyed, and is difficult to restore.
The heavy metal pollution of soil of China high altitude localities is serious at present, although having centainly for heavy metal over-accumulated plant Research, but this plant growth is slow, is not suitable for promoting in high altitude localities.Based on this, plantation has preventing from heavy metal activity The alfalfa for being suitable for the growth of high altitude localities is significant to heavy metal soil remediation, maintenance plateau ecology key.
Alfalfa is to cultivate one of relatively early, most commonly used alfalfa of plantation, Alfalfa Root System hair in the world It reaches, branch is more, and biomass is big, has the good characteristics such as cold-resistant, saline-alkali tolerant, has weight in improving the ecological environment, conceding the land to forestry It acts on.Although the study found that the alfalfa being grown in heavy metal soil have for other most plants compared with High tolerance level, but the heavy metal of high concentration is inhibited to the sprouting of alfalfa seed, at present China Gao Hai Area's heavy metal pollution of rising sheer from level ground increasingly aggravates, and commercially available alfalfa seed can not normally have been sprouted under field conditions (factors), in order to protect The ecological environment of high altitude localities worsening promotes the development of high altitude localities animal husbandry, improves the pale reddish brown lucerne in high altitude localities Mu preventing from heavy metal germinating capacity is extremely urgent.
Summary of the invention
The object of the present invention is to provide a kind of high altitude localities preventing from heavy metal Alfalfa Growing methods, to solve height The problems such as Altitude Regions heavy-metal contaminated soil Growth of Alfalfa is bad, animal husbandry development is limited, the ecosystem deteriorates, furthermore The ability that alfalfa seed is sprouted and grown in the soil of heavy metal pollution can be improved in the present invention, keeps high altitude localities ecological System is restored, and the ecosystem is optimized.
To achieve the goals above, the technical solution adopted by the present invention is that:
A kind of high altitude localities preventing from heavy metal Alfalfa Growing method, this method include the following steps:
Sowing surface management: moisture in the soil has been retained according to kind preceding crop, soil surface treatment has been carried out to seed bed, before fully erased Stubble crop underground rhizome, soil is whole thin whole broken, and raking, it is smooth to reach native face.
Seed disinfection: carrying out disinfection to alfalfa seed using 2%NaClO solution, then uses distilled water flushing 3 times, 75% ethyl alcohol is reused by seed disinfection 30s, is dried with being placed on the gauze sterilized after distilled water flushing 3 times.
Seed treatment: alfalfa seed is impregnated in the liquor potassic permanganate of 200-500mg/L, after 6 hours, after will Alfalfa seed impregnates 3-6 days in the strain soil liquid.
Sowing: sowing row is sorted out with line-spacing 14-16cm, sowing row depth 1-2cm, band base manure broadcasts alfalfa seed Kind；Sowing, which is finished, to be covered seed with thin soil and treads；After sowing in 3-5 days, at the sowing near spray oxalic acid solution.
It top dressing and cuts weeds: top dressing is carried out when Growth of Alfalfa layer height is more than 15 centimetres, to alfalfa after top dressing Watering is primary；And carry out weeds processing；
Grassland utilization: can start to herd or gather in when Growth of Alfalfa is at least up to 50 centimetres to layer height, when harvesting Pay attention to 10 centimetres of toothing.
The strain soil liquid includes: high altitude localities heavy metal free Polluted Soil, bacillus subtilis, rhizobium and steaming Distilled water.
The oxalic acid solution is the oxalic acid aqueous solution of 2-6mol/l.
The preparation method of the strain soil liquid is to be uniformly mixed 50g planting soil with rhizobium, be dissolved in 25 DEG C, In 1000ml distilled water, stir evenly, 2) bacillus subtilis is added, it stirs evenly.
The solubility that the rhizobial nodule bacterium mixes with soil is hundred million/g of 6-8.
The bacillus subtilis bacterial content is 5 × 1010cfu/ml。
Alfalfa Growing method of the present invention is compared with the traditional method, and has the advantages that preparation contains rhizobium Alfalfa seed is impregnated with the mixed bacteria soil liquid of bacillus subtilis, alfalfa germinating capacity can be improved.In purple Russian fenugreek herb seed was sowed in 3-5 days, and oxalic acid aqueous solution is sprayed at sowing, alfalfa germinating capacity can be improved.In sowing 3-5 The colonization ability of rhizobium and bacillus subtilis is not influenced in it in sprinkling oxalic acid aqueous solution.
Rhizobium can promote a kind of Gram-negative aerobasilus of anomaly of plants hyperplasia, and bacillus subtilis is available In the microorganism system for improving soil, soil hardening, solidification, to improve the yield of crops are reduced.Bacillus subtilis bacterium The antibacterial substances such as subtilin, polymyxins, nystatin, gramicidins that body generates during the growth process, while growing In with other strains compete nutriment, different strains shares the conflict that both may cause.Stringent control in the present invention The concentration of rhizobium and bacillus subtilis processed makes the two reach balance in the soil, and the two that is independent of each other is to alfalfa Facilitation.
Oxalic acid is a kind of organic acid, it is known that working as, pH value in soil is lower, and when soil acidity is higher, raw to plant having Apparent inhibiting effect, such as influence of the acid rain to plant growth.For many years by the constraint of traditional thought, this field researcher The promotion under developing alkaline condition to plant growth is always striven to, alleviates destruction to alleviate more serious ecology in recent years, and It is fresh that acid environment is used to treat to promote plant growth soil less.Binding isotherm speculates hair to inventor through a large number of experiments Existing, oxalic acid can form chelate with heavy metal in the soil, change heavy metal ion in the intracorporal transhipment of plant, increase plant To the tolerance of heavy metal, thus the present invention is opened up.
Specific embodiment:
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention Technical solution be clearly and completely described, the person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds The condition of view carries out, reagents or instruments used without specified manufacturer, is the conventional production that can be obtained by commercially available purchase Product.The planting soil referred in embodiment 1-7 is high altitude localities heavy metal free Polluted Soil.
Embodiment 1
In high altitude localities, 4 pieces of setting of heavy metal free pollution, are denoted as A, B, C, D, respectively according to the following steps by 1 mu every piece It carries out:
The preparation A soil liquid: planting soil 50g is dissolved in 25 DEG C, in 1000ml distilled water, is stirred evenly.
The preparation B soil liquid: planting soil 50g is dissolved in 25 DEG C, in 1000ml distilled water, is stirred evenly, 2) withered grass is added Bacillus stirs evenly, and bacillus subtilis bacterial content is (5 × 1010)cfu/ml.Prepare the bacillus subtilis soil liquid.
The preparation C soil liquid: planting soil 50g is uniformly mixed with rhizobium, and root nodule bacterial content is hundred million/g of 6-8, is dissolved in 25 DEG C, in 1000ml distilled water, stir evenly.Prepare the rhizobium soil liquid.
The preparation D soil liquid: planting soil 50g is uniformly mixed with rhizobium, and root nodule bacterial content is hundred million/g of 6-8, is dissolved in 25 DEG C, in 1000ml distilled water, stir evenly, 2) be added bacillus subtilis, stir evenly, bacillus subtilis bacterial content be (5 × 1010)cfu/ml。
Prepare rhizobium and bacillus subtilis mixed soil solution, i.e., the strain soil liquid of the invention.
Sowing surface management: having retained moisture in the soil according to after kind preceding crop, only carried out soil surface treatment to seed bed, completely clear Except preceding crop underground rhizome, soil is whole thin whole broken, and raking, it is smooth to reach native face.
Seed disinfection: carrying out disinfection to alfalfa seed using 2%NaCLO solution, then uses distilled water flushing 3 times, 75% ethyl alcohol is reused by seed disinfection 30s, is dried with being placed on the gauze sterilized after distilled water flushing 3 times.
Seed treatment: alfalfa seed is impregnated in the liquor potassic permanganate of 200-500mg/L, after 6 hours, respectively The seed on the tetra- pieces of ground A, B, C, D is impregnated 3-6 days in above-mentioned corresponding A, B, C, D soil liquid.
Sowing: sowing row is sorted out with line-spacing 14-16cm, sowing row depth 1-2cm, band base manure broadcasts alfalfa seed Kind；Sowing, which is finished, to be covered seed with thin soil and treads.
The alfalfa germination percentage in the experimental plot A, B, C, D is counted, the results are shown in Table 1.
Table 1 (unit: %)
A B C D
65.22±2.53 72.25±2.12 74.89±1.98 89.56±2.35
According to result shown in table 1 it is found that B, C, D alfalfa germination percentage are higher than A, wherein B and C germination percentage is substantially suitable, D Germination percentage is significantly higher than A, B, C.Illustrate to can be improved after rhizobium and the bacillus subtilis soil liquid impregnate alfalfa seed Percentage of seedgermination.
Embodiment 2
In high altitude localities, heavy metal free contaminated land is arranged 4 groups, every group 4 pieces, 1 mu every piece, is denoted as tetra- groups of A, B, C, D, It is carried out respectively according to the following steps:
Sowing surface management: having retained moisture in the soil according to after kind preceding crop, only carried out soil surface treatment to seed bed, completely clear Except preceding crop underground rhizome, soil is whole thin whole broken, and raking, it is smooth to reach native face.
Seed disinfection: carrying out disinfection to alfalfa seed using 2%NaCLO solution, then uses distilled water flushing 3 times, 75% ethyl alcohol is reused by seed disinfection 30s, is dried with being placed on the gauze sterilized after distilled water flushing 3 times.
Seed treatment: alfalfa seed is impregnated in the liquor potassic permanganate of 200-500mg/L.
The preparation A group soil liquid: planting soil 50g is dissolved in 25 DEG C, in 1000ml distilled water, is stirred evenly.
The preparation B group soil liquid: planting soil 50g being dissolved in 25 DEG C, in 1000ml distilled water, is stirred evenly, 2) addition is withered Careless bacillus, stirs evenly, and bacillus subtilis bacterial content is (5 × 1010)cfu/ml.It is molten to prepare bacillus subtilis soil bacteria Liquid.
The preparation C group soil liquid: planting soil 50g is uniformly mixed with rhizobium, root nodule bacterial content is that root nodule bacterial content is Hundred million/g of 6-8, is dissolved in 25 DEG C, in 1000ml distilled water, stirs evenly.Prepare the rhizobium soil liquid.
The native solution of preparation D group: planting soil 50g is uniformly mixed with rhizobium, root nodule bacterial content is that root nodule bacterial content is 6-8 Hundred million/g, is dissolved in 25 DEG C, in 1000ml distilled water, stirs evenly, 2) bacillus subtilis is added, it stirs evenly, bacillus subtilis Bacterial content is (5 × 1010)cfu/ml。
Prepare rhizobium and bacillus subtilis mixed soil solution.
Seed treatment: alfalfa seed is impregnated in the liquor potassic permanganate of 200-500mg/L, after 6 hours, respectively Tetra- groups of A, B, C, D of seed is impregnated 3-6 days in above-mentioned tetra- groups of soil solution of corresponding A, B, C, D.
Sowing: sowing row is sorted out with line-spacing 14-16cm, sowing row depth 1-2cm, band base manure broadcasts alfalfa seed Kind；Sowing, which is finished, to be covered seed with thin soil and treads.After sowing in 3-5 days, the every group of 4 pieces of ground A, B, C, D are being sowed respectively Nearby spray the oxalic acid aqueous solution of 2mol/l, 4mol/l, 6mol/l, 8mol/l in place.
The alfalfa germination percentage in every piece of experimental plot is counted, the results are shown in Table 2.
Table 2 (unit: %)
According to table 2 as a result, compared with 1 result of table, when the oxalic acid aqueous solution of sprinkling 2mol/l, 4mol/l, 6mol/l When, promote the effect of alfalfa seed germination to have not significant impact rhizobium and bacillus subtilis, but oxalic acid solution is dense When degree reaches 8mol/l, the effect of alfalfa seed germination is promoted to have certain inhibition to make rhizobium and bacillus subtilis With.
Embodiment 3
3 groups of experimental plots of heavy metal free pollution setting in high altitude localities, every group of 6 pieces of experimental plot, 1 mu every piece.According to following Step carries out:
Sowing surface management: having retained moisture in the soil according to after kind preceding crop, only carried out soil surface treatment to seed bed, completely clear Except preceding crop underground rhizome, soil is whole thin whole broken, and raking, it is smooth to reach native face.
Experimental plot 1-6 block, respectively spray cadmium solubility be 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, The cadmium salt soln of 400mg/l simulates cadmium pollution experimental plot.
Experimental plot 7-12, spraying lead concentration respectively is 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, 400mg/ The lead salt solution of l simulates lead contamination experimental plot.
Experimental plot 13-18, respectively spray aluminum concentration be 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, The aluminum salt solution of 400mg/l simulates lead contamination experimental plot.
Seed disinfection: carrying out disinfection to alfalfa seed using 2%NaCLO solution, then uses distilled water flushing 3 times, 75% ethyl alcohol is reused by seed disinfection 30s, is dried with being placed on the gauze sterilized after distilled water flushing 3 times.
Seed treatment: alfalfa seed is impregnated in the liquor potassic permanganate of 200-500mg/L, after 6 hours, after will Alfalfa seed impregnates 3-6 days in the strain soil liquid.
The strain soil liquid is by rhizobium, bacillus subtilis, planting soil solution mixing system at preparation method It is 1) planting soil 50g is uniformly mixed with rhizobium, root nodule bacterial content is that root nodule bacterial content is hundred million/g of 6-8,25 DEG C are dissolved in, In 1000ml distilled water, stir evenly, 2) be added bacillus subtilis, stir evenly, bacillus subtilis bacterial content be (5 × 1010)cfu/ml。
Sowing: sowing row is sorted out with line-spacing 14-16cm, sowing row depth 1-2cm, band base manure broadcasts alfalfa seed Kind；Sowing, which is finished, to be covered seed with thin soil and treads.
The alfalfa germination percentage in every group of experimental plot is counted, the results are shown in Table 3.
Table 3 (unit: %)
As a result, the germination percentage of alfalfa is reduced with the growth of cadmium solubility, lead concentration, aluminum concentration according to table 3.
Embodiment 4
3 groups of experimental plots of heavy metal free pollution setting in high altitude localities, every group of 6 pieces of experimental plot, 1 mu every piece.According to following Step carries out:
Sowing surface management: having retained moisture in the soil according to after kind preceding crop, only carried out soil surface treatment to seed bed, completely clear Except preceding crop underground rhizome, soil is whole thin whole broken, and raking, it is smooth to reach native face.
Experimental plot 1-6 block, respectively spray cadmium solubility be 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, The cadmium salt soln of 400mg/l simulates cadmium pollution experimental plot.
Experimental plot 7-12, spraying lead concentration respectively is 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, 400mg/ The lead salt solution of l simulates lead contamination experimental plot.
Experimental plot 13-18, respectively spray aluminum concentration be 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, The aluminum salt solution of 400mg/l simulates lead contamination experimental plot.
Seed disinfection: carrying out disinfection to alfalfa seed using 2%NaCLO solution, then uses distilled water flushing 3 times, 75% ethyl alcohol is reused by seed disinfection 30s, is dried with being placed on the gauze sterilized after distilled water flushing 3 times.
Seed treatment: alfalfa seed is impregnated in the liquor potassic permanganate of 200-500mg/L, after 6 hours, after will Alfalfa seed impregnates 3-6 days in the strain soil liquid.
The strain soil liquid is by rhizobium, bacillus subtilis, planting soil solution mixing system at preparation method It is 1) planting soil 50g is uniformly mixed with rhizobium, root nodule bacterial content is that root nodule bacterial content is hundred million/g of 6-8,25 DEG C are dissolved in, In 1000ml distilled water, stir evenly, 2) be added bacillus subtilis, stir evenly, bacillus subtilis bacterial content be (5 × 1010)cfu/ml。
Sowing: sowing row is sorted out with line-spacing 14-16cm, sowing row depth 1-2cm, band base manure broadcasts alfalfa seed Kind；Sowing, which is finished, to be covered seed with thin soil and treads；After sowing in 3-5 days, all experimental plots at the sowing near spray Spill 2mol/l oxalic acid aqueous solution.
The alfalfa germination percentage in every group of experimental plot is counted, the results are shown in Table 4.
Table 4 (unit: %)
As a result, after planting spraying the alfalfa of 2mol/l oxalic acid solution, germination percentage is with cadmium solubility, lead according to table 4 The growth of concentration, aluminum concentration reduces, but is significantly increased with compared with concentration does not spray the germination percentage of oxalic acid solution (table 3).
Embodiment 5
3 groups of experimental plots of heavy metal free pollution setting in high altitude localities, every group of 6 pieces of experimental plot, 1 mu every piece.According to following Step carries out:
Sowing surface management: having retained moisture in the soil according to after kind preceding crop, only carried out soil surface treatment to seed bed, completely clear Except preceding crop underground rhizome, soil is whole thin whole broken, and raking, it is smooth to reach native face.
Experimental plot 1-6 block, respectively spray cadmium solubility be 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, The cadmium salt soln of 400mg/l simulates cadmium pollution experimental plot.
Experimental plot 7-12, spraying lead concentration respectively is 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, 400mg/ The lead salt solution of l simulates lead contamination experimental plot.
Experimental plot 13-18, respectively spray aluminum concentration be 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, The aluminum salt solution of 400mg/l simulates lead contamination experimental plot.
Seed disinfection: carrying out disinfection to alfalfa seed using 2%NaCLO solution, then uses distilled water flushing 3 times, 75% ethyl alcohol is reused by seed disinfection 30s, is dried with being placed on the gauze sterilized after distilled water flushing 3 times.
Seed treatment: alfalfa seed is impregnated in the liquor potassic permanganate of 200-500mg/L, after 6 hours, after will Alfalfa seed impregnates 3-6 days in the strain soil liquid.
Sowing: sowing row is sorted out with line-spacing 14-16cm, sowing row depth 1-2cm, band base manure broadcasts alfalfa seed Kind；Sowing, which is finished, to be covered seed with thin soil and treads；After sowing in 3-5 days, at the sowing near spray 4mol/l oxalic acid Aqueous solution.
The strain soil liquid is by rhizobium, bacillus subtilis, planting soil solution mixing system at preparation method It is 1) planting soil 50g is uniformly mixed with rhizobium, root nodule bacterial content is that root nodule bacterial content is hundred million/g of 6-8,25 DEG C are dissolved in, In 1000ml distilled water, stir evenly, 2) be added bacillus subtilis, stir evenly, bacillus subtilis bacterial content be (5 × 1010)cfu/ml。
The alfalfa germination percentage in every group of experimental plot is counted, the results are shown in Table 5.
Table 5 (unit: %)
As a result, after planting spraying the alfalfa of 4mol/l oxalic acid solution, germination percentage is with cadmium solubility, lead according to table 5 The growth of concentration, aluminum concentration reduces, but does not spray oxalic acid solution (table 3) with same concentration and after planting spray 2mol/l grass with concentration The germination percentage of the alfalfa of acid solution (table 4) is compared and is significantly increased.
Embodiment 6
3 groups of experimental plots of heavy metal free pollution setting in high altitude localities, every group of 6 pieces of experimental plot, 1 mu every piece.According to following Step carries out:
Sowing surface management: having retained moisture in the soil according to after kind preceding crop, only carried out soil surface treatment to seed bed, completely clear Except preceding crop underground rhizome, soil is whole thin whole broken, and raking, it is smooth to reach native face.
Experimental plot 1-6 block, respectively spray cadmium solubility be 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, The cadmium salt soln of 400mg/l simulates cadmium pollution experimental plot.
Experimental plot 7-12, spraying lead concentration respectively is 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, 400mg/ The lead salt solution of l simulates lead contamination experimental plot.
Experimental plot 13-18, respectively spray aluminum concentration be 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, The aluminum salt solution of 400mg/l simulates lead contamination experimental plot.
Seed disinfection: carrying out disinfection to alfalfa seed using 2%NaCLO solution, then uses distilled water flushing 3 times, 75% ethyl alcohol is reused by seed disinfection 30s, is dried with being placed on the gauze sterilized after distilled water flushing 3 times.
Seed treatment: alfalfa seed is impregnated in the liquor potassic permanganate of 200-500mg/L, after 6 hours, after will Alfalfa seed impregnates 3-6 days in the strain soil liquid.
Sowing: sowing row is sorted out with line-spacing 14-16cm, sowing row depth 1-2cm, band base manure broadcasts alfalfa seed Kind；Sowing, which is finished, to be covered seed with thin soil and treads；After sowing in 3-5 days, at the sowing near spray 6mol/l oxalic acid Aqueous solution.
The strain soil liquid is by rhizobium, bacillus subtilis, planting soil solution mixing system at preparation method It is 1) planting soil 50g is uniformly mixed with rhizobium, root nodule bacterial content is that root nodule bacterial content is hundred million/g of 6-8,25 DEG C are dissolved in, In 1000ml distilled water, stir evenly, 2) be added bacillus subtilis, stir evenly, bacillus subtilis bacterial content be (5 × 1010)cfu/ml。
The alfalfa germination percentage in every group of experimental plot is counted, the results are shown in Table 6.
Table 6 (unit: %)
As a result, after planting spraying the alfalfa of 6mol/l oxalic acid solution, germination percentage is with cadmium solubility, lead according to table 6 The growth of concentration, aluminum concentration reduces, but does not spray oxalic acid solution (table 3) with same concentration, after planting sprays 2mol/l grass with concentration Acid solution (table 4) and have compared with concentration after planting sprays the germination percentage of the alfalfa of 4mol/l oxalic acid solution (table 5) bright It is aobvious to increase.
Embodiment 7
3 groups of experimental plots of heavy metal free pollution setting in high altitude localities, every group of 6 pieces of experimental plot, 1 mu every piece.According to following Step carries out:
Sowing surface management: having retained moisture in the soil according to after kind preceding crop, only carried out soil surface treatment to seed bed, completely clear Except preceding crop underground rhizome, soil is whole thin whole broken, and raking, it is smooth to reach native face.
Experimental plot 1-6 block, respectively spray cadmium solubility be 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, The cadmium salt soln of 400mg/l simulates cadmium pollution experimental plot.
Experimental plot 7-12, spraying lead concentration respectively is 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, 400mg/ The lead salt solution of l simulates lead contamination experimental plot.
Experimental plot 13-18, respectively spray aluminum concentration be 0mg/l, 10mg/l, 50mg/l, 100mg/l, 200mg/l, The aluminum salt solution of 400mg/l simulates lead contamination experimental plot.
Seed disinfection: carrying out disinfection to alfalfa seed using 2%NaCLO solution, then uses distilled water flushing 3 times, 75% ethyl alcohol is reused by seed disinfection 30s, is dried with being placed on the gauze sterilized after distilled water flushing 3 times.
Seed treatment: alfalfa seed is impregnated in the liquor potassic permanganate of 200-500mg/L, after 6 hours, after will Alfalfa seed impregnates 3-6 days in the strain soil liquid.
Sowing: sowing row is sorted out with line-spacing 14-16cm, sowing row depth 1-2cm, band base manure broadcasts alfalfa seed Kind；Sowing, which is finished, to be covered seed with thin soil and treads；After sowing in 3-5 days, at the sowing near spray 8mol/l oxalic acid Aqueous solution.
The strain soil liquid is by rhizobium, bacillus subtilis, planting soil solution mixing system at preparation method It is 1) planting soil 50g is uniformly mixed with rhizobium, root nodule bacterial content is that root nodule bacterial content is hundred million/g of 6-8,25 DEG C are dissolved in, In 1000ml distilled water, stir evenly, 2) be added bacillus subtilis, stir evenly, bacillus subtilis bacterial content be (5 × 1010)cfu/ml。
The alfalfa germination percentage in every group of experimental plot is counted, the results are shown in Table 7.
Table 7 (unit: %)
As a result, after planting spraying the alfalfa of 8mol/l oxalic acid solution, germination percentage is with cadmium solubility, lead according to table 7 Concentration, aluminum concentration growth reduce, with compared with concentration does not spray oxalic acid solution (table 3) germination percentage remain basically stable, but with dense Degree after planting spray 2mol/l oxalic acid solution (table 4) and after planting sprayed with concentration 4mol/l oxalic acid solution (table 5) and It decreased significantly compared with concentration after planting sprays the germination percentage of the alfalfa of 6mol/l oxalic acid solution (table 6).
According to the experimental result of embodiment 3-7 it is found that spraying 2-6mol/l in the soil after sowing alfalfa seed Oxalic acid solution can promote germination of the alfalfa seed in heavy-metal contaminated soil, reach 8mol/l in oxalic acid solution concentration When facilitation be substantially reduced.

Claims (6)

1. a kind of high altitude localities preventing from heavy metal Alfalfa Growing method, which comprises the following steps:
1) it sows surface management: having retained moisture in the soil according to kind preceding crop, soil surface treatment, fully erased preceding stubble are carried out to seed bed Crop underground rhizome, soil is whole thin whole broken, and raking, it is smooth to reach native face；
2) seed disinfection: carrying out disinfection to alfalfa seed using NaClO solution, then uses distilled water flushing, reuses 75% ethyl alcohol dries seed disinfection with being placed on the gauze sterilized after distilled water flushing；
3) seed treatment: alfalfa seed is impregnated in liquor potassic permanganate, and after 6 hours, alfalfa seed is being contained Have in the strain soil liquid of bacillus subtilis and rhizobium and impregnates 3-6 days；
4) sow: band base manure sows alfalfa seed；Sowing, which is finished, to be covered seed with thin soil and treads；3- after sowing In 5 days, at the sowing near spray oxalic acid solution；
5) it top dressing and cuts weeds: carrying out top dressing when Growth of Alfalfa layer height is more than 15 centimetres, alfalfa is poured after top dressing Water is primary；And carry out weeds processing.
2. implantation methods as described in claim 1, which is characterized in that the strain soil liquid includes: planting soil, withered grass bud Spore bacillus, rhizobium and distilled water.
3. implantation methods as claimed in claim 1 or 2, which is characterized in that the oxalic acid solution is the oxalic acid water of 2-6mol/L Solution.
4. implantation methods as claimed in claim 3, which is characterized in that the preparation method of the strain soil liquid is, by 50g Planting soil is uniformly mixed with rhizobium, is dissolved in 25 DEG C, in 1000ml distilled water, is stirred evenly, 2) bacillus subtilis is added, it stirs It mixes uniformly.
5. implantation methods as claimed in claim 4, which is characterized in that the solubility that the rhizobium mix with the planting soil is Hundred million/g of 6-8.
6. implantation methods as claimed in claim 5, which is characterized in that the bacillus subtilis bacterial content is 5 × 1010cfu/ ml。
CN201811481688.4A 2018-12-05 2018-12-05 A kind of high altitude localities preventing from heavy metal Alfalfa Growing method Pending CN109588080A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110073906A (en) * 2019-06-18 2019-08-02 牟钰德 One kind sweeps along nourishing slurry inlaying process to sow herbaceous crops reparation degeneration Grassland desertification method
CN112369156A (en) * 2020-11-12 2021-02-19 安徽省农业科学院畜牧兽医研究所 Efficient yield-increasing treatment method for alfalfa seeds

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1951197A (en) * 2006-11-01 2007-04-25 沈阳农业大学 A novel method for controlling soybean root disease by using rhizobia
CN101194567A (en) * 2008-01-03 2008-06-11 中国农业大学 Method for improving production volume and quality of alfalfa seed
CN102559198A (en) * 2010-12-14 2012-07-11 华中农业大学 Passivator for controlling pollutions of heavy metals copper, lead and cadmium on soil and applications thereof
CN104255110A (en) * 2014-09-04 2015-01-07 兰州大学 Method for promoting germination of alfalfa seeds
CN107046840A (en) * 2017-01-23 2017-08-18 中国科学院沈阳应用生态研究所 A kind of cadmium pollution farming land safe utilization method based on barrier layer
CN107432151A (en) * 2017-08-06 2017-12-05 菏泽市农业科学院 A kind of application technique of the efficient machine dispenser fertilizer of Peanut Mild letter
CN108213074A (en) * 2018-01-10 2018-06-29 西北农林科技大学 A kind of chelating agent strengthens the method that arbor repairs drought-hit area basic soil lead contamination
CN108277173A (en) * 2018-01-12 2018-07-13 西北农林科技大学 The double of plant resistance to environment stress connect bacterium method in a kind of raising heavy-metal contaminated soil

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1951197A (en) * 2006-11-01 2007-04-25 沈阳农业大学 A novel method for controlling soybean root disease by using rhizobia
CN101194567A (en) * 2008-01-03 2008-06-11 中国农业大学 Method for improving production volume and quality of alfalfa seed
CN102559198A (en) * 2010-12-14 2012-07-11 华中农业大学 Passivator for controlling pollutions of heavy metals copper, lead and cadmium on soil and applications thereof
CN104255110A (en) * 2014-09-04 2015-01-07 兰州大学 Method for promoting germination of alfalfa seeds
CN107046840A (en) * 2017-01-23 2017-08-18 中国科学院沈阳应用生态研究所 A kind of cadmium pollution farming land safe utilization method based on barrier layer
CN107432151A (en) * 2017-08-06 2017-12-05 菏泽市农业科学院 A kind of application technique of the efficient machine dispenser fertilizer of Peanut Mild letter
CN108213074A (en) * 2018-01-10 2018-06-29 西北农林科技大学 A kind of chelating agent strengthens the method that arbor repairs drought-hit area basic soil lead contamination
CN108277173A (en) * 2018-01-12 2018-07-13 西北农林科技大学 The double of plant resistance to environment stress connect bacterium method in a kind of raising heavy-metal contaminated soil

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
CLI´STENES WILLIAMS A.DO NASCIMENTO等: "Comparison of natural organic acids and synthetic chelates at enhancing phytoextraction of metals from a multi-metal contaminated soil", 《ENVIRONMENTAL POLLUTION》 *
严莲荷: "《水处理药剂及配方手册》", 31 January 2004, 中国石化出版 *
刘欢: "外源低分子量有机酸对镉胁迫下美洲商陆的超累积和解Cd毒的机制研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 *
李锦华: "《西藏牧草繁育研究进展》", 31 March 2017, 甘肃科学技术出版社 *
杨海琳等: "低分子有机酸去除土壤中重金属条件的研究", 《农业环境科学学报》 *
殷朝洲: "高锰酸钾处理苜蓿种子效果好", 《中国草地》 *
甘肃省农牧厅: "《牧草种植与草原保护技术读本》", 30 November 2014 *
罗巧芝: "适宜于青藏高原种植的耐镉牧草品种筛选", 《中国优秀硕士学位论文全文数据库 农业科技辑》 *
金婷婷等: "外源有机酸对铝毒胁迫下大豆根系形态的影响", 《中国油料作物学报》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110073906A (en) * 2019-06-18 2019-08-02 牟钰德 One kind sweeps along nourishing slurry inlaying process to sow herbaceous crops reparation degeneration Grassland desertification method
CN110073906B (en) * 2019-06-18 2021-04-27 牟钰德 Method for repairing degraded desertified grassland by planting herbaceous crops by wrapping and embedding nutrient slurry
CN112369156A (en) * 2020-11-12 2021-02-19 安徽省农业科学院畜牧兽医研究所 Efficient yield-increasing treatment method for alfalfa seeds

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