CN109576282B - 月季转录因子RhMYB4及其花器官发育调控中的应用 - Google Patents
月季转录因子RhMYB4及其花器官发育调控中的应用 Download PDFInfo
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- CN109576282B CN109576282B CN201811549304.8A CN201811549304A CN109576282B CN 109576282 B CN109576282 B CN 109576282B CN 201811549304 A CN201811549304 A CN 201811549304A CN 109576282 B CN109576282 B CN 109576282B
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- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
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- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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Abstract
Description
常用菌株或质粒 | 型号或名称 |
大肠杆菌 | top10 |
农杆菌 | GV3101 |
酵母菌 | Y2H GOLD(黄金酵母)、AH109 |
克隆载体 | pMD18-T |
过表达载体 | Super1300-GFP(C) |
VIGS载体 | pTRV1、pTRV2 |
酵母载体 | pBD-GAL4、pGBKT7、pGADT7(Clontech公司) |
50%PEG | 240μL |
1M LiAc | 36μL |
ssDNA(5mg/mL) | 10μL |
重组质粒 | 1μL |
Claims (3)
Priority Applications (1)
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CN201811549304.8A CN109576282B (zh) | 2018-12-18 | 2018-12-18 | 月季转录因子RhMYB4及其花器官发育调控中的应用 |
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CN201811549304.8A CN109576282B (zh) | 2018-12-18 | 2018-12-18 | 月季转录因子RhMYB4及其花器官发育调控中的应用 |
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Publication Number | Publication Date |
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CN109576282A CN109576282A (zh) | 2019-04-05 |
CN109576282B true CN109576282B (zh) | 2021-08-10 |
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Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112159865B (zh) * | 2020-10-15 | 2022-05-06 | 北京市林业果树科学研究院 | 与月季红色花瓣相关的Indel标记及鉴定月季红色花瓣的分子方法 |
CN112522280B (zh) * | 2020-12-07 | 2023-05-16 | 上海师范大学 | 一种用于调控小兰屿蝴蝶兰花瓣颜色的基因PeMYB4序列及其应用 |
CN112724213B (zh) * | 2021-01-13 | 2022-02-18 | 中国农业大学 | 甘薯花青苷合成和抗逆相关蛋白IbMYB4及其编码基因与应用 |
CN113151306B (zh) * | 2021-06-09 | 2022-05-17 | 浙江农林大学 | 一种提高梅花花瓣抗寒性的基因PmWRKY57及其应用 |
CN114410646B (zh) * | 2021-12-13 | 2023-09-29 | 上海师范大学 | 一种调控蝴蝶兰花器官发育的基因PeARF18及其应用 |
CN114907463A (zh) * | 2022-05-11 | 2022-08-16 | 中国农业科学院棉花研究所 | 瑟伯氏棉基因GthCBF4互作蛋白及其筛选方法 |
CN116217683B (zh) * | 2022-09-08 | 2024-04-16 | 深圳全棉时代科技有限公司 | 一种与棉花纤维品质相关的基因、超表达载体和敲除载体及应用 |
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JP2005192483A (ja) * | 2004-01-07 | 2005-07-21 | Japan Science & Technology Agency | 植物の雄性不稔体の生産方法およびこれを用いて得られる植物体、並びにその利用 |
CN103421835B (zh) * | 2012-12-08 | 2015-03-04 | 华中农业大学 | 转录因子基因RrMYB11在调控玫瑰花型中的应用 |
CN103060340B (zh) * | 2013-01-30 | 2014-04-16 | 山东省花生研究所 | 一种花生中逆境胁迫AhMYBL6基因的克隆及功能表达方法 |
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