CN109549862B - Cosmetic containing growth factor and preparation method thereof - Google Patents
Cosmetic containing growth factor and preparation method thereof Download PDFInfo
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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Abstract
The invention discloses a cosmetic containing growth factors and a preparation method thereof, wherein the preparation method comprises the following steps: (1) adding water and disodium ethylene diamine tetraacetate into a water phase pot, stirring and mixing uniformly, then adding glycerol, 1, 3-propylene glycol, cetearyl alcohol, plant extract and hydroxyethyl cellulose, stirring and mixing uniformly; (2) adding glycerol monostearate, sorbitan monopalmitate and shea butter into an oil phase pot, and stirring and mixing uniformly; (3) and (4) homogenizing. The cosmetic containing the growth factors and the preparation method thereof are safe and non-irritant, have the functions of nutrition and moistening, have the special functions of nutrition, anti-aging, stable emulsification, antibiosis and anti-inflammation, promotion of wound healing, repair of damaged cells and the like, have the effects of high-efficiency moisture retention and lasting water retention, can continuously keep the moisture content of the horny layer of the skin, ensure the moistening degree of the skin, enable the skin to be compact, rich in elasticity and luster, and enable the skin to be more exquisite, smooth and full of elasticity after being frequently used.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a cosmetic containing growth factors and a preparation method thereof.
Background
The cosmetic is applied to any part of human body surface (skin, nail, lip, oral cavity membrane, etc.) by smearing, spraying or other similar methods, so as to achieve the purposes of cleaning, eliminating unpleasant odor, caring skin, and finishing.
Sebum is skin oil that is produced by sebaceous gland cells inside the skin and then secreted to the surface of the skin. The study demonstrated that the composition of sebum is roughly as follows: about 57% triglyceride or fatty acid, about 12% squalene, about 3% sterol ester, about 23% wax ester and 1-2% cholesterol.
Dermatologists and cosmetic experts have long sought various natural or synthetic substances that are effective in caring human skin, or treating certain skin disorders. Nowadays, cosmetics have been developed to a relative prosperous stage, and cosmetics and skin care products in various forms and functions are gladly full. Among a plurality of skin care products, a product containing natural non-irritant components and a formula which can really and effectively maintain skin components is always pursued by skin care product manufacturers and skin care product consumers. Skin care products that can actually meet this need often become expensive luxuries that are not the subject of most human consumption.
Moisture is one of important substances for human bodies to live and is also one of important components of the human bodies, and the life activities such as metabolism of the human bodies lacking moisture can be affected, for example, 10% of water loss of the human bodies threatens the health, and 20% of water loss of the human bodies can lead to death. The skin is one of the important organs of the human body, and the influence and the injury of the skin caused by water shortage are not negligible. The skin covered on the surface of a human body is affected most by external factors such as environment and the like, so that the skin is very easy to lose moisture, and the skin loses elasticity after being lack of water, and the repair and regeneration speed is reduced, so that the skin problems such as shrivelled, aged and poor in elasticity occur, and therefore the skin moisturizing repair becomes a theme of modern cosmetics.
In addition, in recent years, the cosmetic industry advocates natural functional components and natural plant extracts with moisturizing or antioxidant effects to be added into various skin care cosmetics so as to resist skin aging, which is an important research direction of the current anti-aging cosmetics.
Disclosure of Invention
The invention mainly solves the technical problem of providing a cosmetic containing growth factors and a preparation method thereof, and the cosmetic has long-acting skin moisturizing, whitening, antibacterial and antioxidant effects.
In order to solve the technical problems, the invention adopts the technical scheme that:
a method for preparing cosmetic containing growth factor comprises the following steps:
(1) adding water and disodium ethylene diamine tetraacetate into a water phase pot, stirring and mixing uniformly, then adding glycerol, 1, 3-propylene glycol, cetearyl alcohol, plant extract and hydroxyethyl cellulose, stirring and mixing uniformly to obtain a water phase material;
(2) adding glycerol monostearate, sorbitan monopalmitate and shea butter into an oil phase pot, and stirring and mixing uniformly to obtain an oil phase material;
(3) pumping the water phase material into an emulsifying pot, pumping the oil phase material while stirring and homogenizing, and finally adding the moisturizing additive and the growth factor and mixing uniformly to obtain the water-based emulsion.
A method for preparing cosmetic containing growth factor comprises the following steps:
(1) adding 50-70 parts by weight of water and 0.05-0.15 part by weight of disodium ethylene diamine tetraacetate into a water phase pot, stirring and mixing uniformly, then adding 5-10 parts by weight of glycerol, 1-4 parts by weight of 1, 3-propylene glycol, 0.5-1.5 parts by weight of cetostearyl alcohol, 9-11 parts by weight of honeysuckle extract, 4-6 parts by weight of dipsacus asperoides extract and 0.5-1.5 parts by weight of hydroxyethyl cellulose modifier, heating to 55-85 ℃, stirring and mixing uniformly to obtain a water phase material;
(2) adding 0.5-3 parts by weight of glycerol monostearate, 0.5-3 parts by weight of sorbitan monopalmitate and 1-4 parts by weight of shea butter into an oil phase pot, heating to 55-85 ℃, and uniformly stirring and mixing to obtain an oil phase material;
(3) pumping the water phase material into an emulsifying pot, pumping the oil phase material while stirring and homogenizing, homogenizing at 55-85 deg.C for 3-13 min, keeping the temperature for 10-20min, cooling to room temperature, adding 0.5-1.5 weight parts of moisturizing additive and 1-5 weight parts of growth factor, and mixing well to obtain the final product.
The growth factor is a stem cell growth factor. The stem cell growth factor comprises epidermal growth factor, fibroblast growth factor, platelet-derived growth factor and vascular endothelial growth factor.
The hydroxyethyl cellulose modifier is prepared by the following method: hydroxyethyl cellulose and water are mixed according to the mass ratio of 1: (10-30) stirring at the rotation speed of 200-; and (4) spray-drying the reaction solution to obtain the catalyst.
The air inlet temperature of the spray drying is 170-190 ℃, the air outlet temperature is 70-90 ℃, and the air speed of the hot air is2-5m3The material feeding speed is 25-45mL/min, and the rotating speed of the atomizer is 200-500 r/min.
The honeysuckle extracting solution is prepared by the following method: pulverizing flos Lonicerae to 10-20 mesh to obtain flos Lonicerae powder; mixing honeysuckle powder and water according to a mass ratio of 1: (5-15), ultrasonically extracting for 2-5h at the temperature of 45-65 ℃, centrifuging to remove bottom sediment, and filtering supernate with a ceramic membrane of 0.1-0.5 mu m to obtain the product.
The dipsacus root extract is prepared by the following method: pulverizing radix Dipsaci to 10-20 mesh to obtain radix Dipsaci powder; mixing the teasel root powder and 10-20wt% of ethanol water solution according to the mass ratio of 1: (5-15), ultrasonically extracting for 2-5h at the temperature of 45-65 ℃, centrifuging to remove bottom sediment, and filtering supernate with a ceramic membrane of 0.1-0.5 mu m to obtain the product.
The ultrasonic power of the ultrasonic extraction is 300-.
The moisturizing additive is at least one of amphiphilic carboxymethyl chitosan octadecyl ammonium chloride and hyaluronic acid quaternary ammonium salt. Further, the moisturizing additive consists of 25-35wt% of amphiphilic carboxymethyl chitosan octadecyl ammonium chloride and 65-75wt% of hyaluronic acid quaternary ammonium salt.
The invention also provides a cosmetic containing the growth factor, which is prepared by the preparation method of the cosmetic containing the growth factor.
The invention has the beneficial effects that:
the honeysuckle extract is extracted from dried flower buds and/or flowers with initial blossoming of Lonicera of Caprifoliaceae and congeneric plants, mainly comprises chlorogenic acid (chlorogenic acid) and Luteolin (Luteolin), can be used as raw materials of medicines, health products and cosmetics, and has the functions of diminishing inflammation and resisting bacteria.
The dipsacus root extract of the present invention has strong water-holding capacity, and can effectively supplement the moisture and humidity required by the skin, so that the skin has good skin feel and luster. The teasel roots are also called teasel roots, monk, mountain radishes and dipsacus roots, and are produced mainly in Sichuan province, Hubei province, Hunan province, Guizhou province and the like.
The epidermal cell growth factor can promote the growth of epidermal cells and repair the epidermis; the fibroblast growth factor can promote the growth of fibroblasts and accelerate the generation of collagen.
The cosmetic containing the growth factors is safe and non-irritant, has the functions of nutrition and moistening, has the special functions of nutrition, anti-aging, stable emulsification, antibiosis and anti-inflammation, promotion of wound healing, repair of damaged cells and the like, also has the effects of high-efficiency moisture retention and lasting water locking, can continuously keep the moisture content of the horny layer of the skin, ensures the moisture degree of the skin, enables the skin to be compact, rich in elasticity and luster, and enables the skin to be more exquisite, smooth and full of elasticity after being frequently used.
Detailed Description
The features and advantages of the present invention will become more apparent and appreciated from the following detailed description of the invention.
The materials used are described below: glycerol, CAS number: 56-81-5. 1, 3-propanediol, CAS No.: 504-63-2. Shea butter is provided by Sichuan Biotech limited. Hydroxyethyl cellulose is provided by the chemical Limited of Jade spill in Jinan, and the product grade is a premium product. Zinc glycinate, CAS number: 7214-08-6. Zinc lactate, i.e., L-zinc lactate, CAS No.: 103404-76-8. Chuan xu is provided by Huimin Baicao medicine marketing limited company in Bozhou city, and the origin is Sichuan. Honeysuckle is provided by the marketing company of the Haemahao Chinese herbal medicine of Bozhou city, and the origin is Chongqing. Disodium ethylenediaminetetraacetic acid, CAS No.: 139-33-3. Glycerol monostearate, CAS number: 31566-31-1. Cetostearyl alcohol, CAS No.: 67762-27-0. Sorbitan monopalmitate, CAS No.: 26266-57-9. Catechin, CAS No.: 7295-85-4. The amphiphilic carboxymethyl chitosan octadecyl ammonium chloride is prepared according to the method shown in example 1 in the Chinese patent with the application number of 200710056993.4. The hyaluronic acid quaternary ammonium salt is prepared according to the method shown in example 1 in Chinese patent with application number 200810016722.0. The vacuum homogenizing and emulsifying machine is a vacuum homogenizing and emulsifying machine with the model of MSE-E, which is provided by Shanghai Mingshi electromechanical equipment, and the specification is 50L.
Example 1
A method for preparing a cosmetic containing growth factors comprises the following steps:
(1) adding 60 parts by weight of deionized water and 0.1 part by weight of disodium ethylene diamine tetraacetate into a water phase pot of a vacuum homogenizing emulsifying machine, stirring for 5min at the rotating speed of 300r/min, uniformly mixing, then adding 8 parts by weight of glycerol, 2.5 parts by weight of 1, 3-propylene glycol, 1 part by weight of cetostearyl alcohol, 10 parts by weight of honeysuckle extract, 5 parts by weight of dipsacus asperoides extract and 1 part by weight of hydroxyethyl cellulose modifier, heating to 75 ℃, stirring for 15min at the rotating speed of 300r/min, and uniformly mixing to obtain a water phase material;
(2) adding 2 parts by weight of glycerol monostearate, 2 parts by weight of sorbitan monopalmitate and 2.5 parts by weight of shea butter into an oil phase pot of a vacuum homogenizing and emulsifying machine, heating to 75 ℃, stirring at the rotating speed of 300r/min for 15min, and uniformly mixing to obtain an oil phase material;
(3) pumping the water phase material into an emulsifying pot of a vacuum homogenizing emulsifying machine, pumping the oil phase material while stirring and homogenizing at 4500r/min, homogenizing at 75 ℃ for 8 minutes, then preserving the temperature for 15 minutes, cooling to room temperature of 25 ℃, adding 1 weight part of hyaluronic acid quaternary ammonium salt and 3 weight parts of growth factors, stirring at the rotating speed of 300r/min for 10 minutes, and uniformly mixing to obtain the cosmetic containing the growth factors.
The growth factor is stem cell growth factor, is prepared according to the method shown in the example 1 in the Chinese patent with the application number of 201510420174.8, and has the following component analysis results: the content of the stem cell growth factor is 33 wt%, and specifically comprises the following components: the content of epidermal growth factor is 18 wt%, the content of fibroblast growth factor is 8 wt%, the content of platelet-derived growth factor is 2 wt%, and the content of vascular endothelial growth factor is 5 wt%.
The hydroxyethyl cellulose modifier is prepared by the following method: hydroxyethyl cellulose and deionized water are mixed according to the mass ratio of 1: 20 stirring and mixing for 15min at the rotating speed of 300r/min, then adding zinc glycinate accounting for 2 percent of the weight of the hydroxyethyl cellulose, zinc lactate accounting for 2 percent of the weight of the hydroxyethyl cellulose and catechin accounting for 2 percent of the weight of the hydroxyethyl cellulose, and stirring for 3h at the rotating speed of 300r/min to obtain a reaction solution; spray drying the reaction solution, wherein the inlet air temperature of the spray drying is 180 ℃, the outlet air temperature is 80 ℃, and the hot air speed is 4m3At the feeding speed of 35mL/min and the rotating speed of an atomizer of 300r/min, the hydroxyethyl fiber is obtainedA modified product of cellulose.
The honeysuckle extracting solution is prepared by the following method: pulverizing flos Lonicerae to 16 mesh to obtain flos Lonicerae powder; mixing honeysuckle powder and deionized water according to a mass ratio of 1: 10, mixing, carrying out ultrasonic extraction at 55 ℃ for 3h, wherein the ultrasonic power of the ultrasonic extraction is 400W, the ultrasonic frequency is 35kHz, then centrifuging at the rotating speed of 6000r/min for 30min to remove bottom sediment, and filtering supernate with a ceramic membrane of 0.45 mu m to obtain the honeysuckle extracting solution.
The dipsacus root extract is prepared by the following method: crushing the teasel roots to 16 meshes to obtain teasel root powder; mixing the teasel root powder and 15 wt% of ethanol water solution according to the mass ratio of 1: 10, mixing, carrying out ultrasonic extraction at 50 ℃ for 3h, wherein the ultrasonic power of the ultrasonic extraction is 400W, the ultrasonic frequency is 35kHz, then centrifuging at the rotating speed of 6000r/min for 30min to remove bottom sediment, and filtering supernate with a ceramic membrane of 0.45 mu m to obtain the dipsacus asperoides extract.
Example 2
A method for preparing a cosmetic containing growth factors comprises the following steps:
(1) adding 60 parts by weight of deionized water and 0.1 part by weight of disodium ethylene diamine tetraacetate into a water phase pot of a vacuum homogenizing emulsifying machine, stirring for 5min at the rotating speed of 300r/min, uniformly mixing, then adding 8 parts by weight of glycerol, 2.5 parts by weight of 1, 3-propylene glycol, 1 part by weight of cetostearyl alcohol, 10 parts by weight of honeysuckle extract, 5 parts by weight of dipsacus asperoides extract and 1 part by weight of hydroxyethyl cellulose, heating to 75 ℃, stirring for 15min at the rotating speed of 300r/min, and uniformly mixing to obtain a water phase material;
(2) adding 2 parts by weight of glycerol monostearate, 2 parts by weight of sorbitan monopalmitate and 2.5 parts by weight of shea butter into an oil phase pot of a vacuum homogenizing and emulsifying machine, heating to 75 ℃, stirring at the rotating speed of 300r/min for 15min, and uniformly mixing to obtain an oil phase material;
(3) pumping the water phase material into an emulsifying pot of a vacuum homogenizing emulsifying machine, pumping the oil phase material while stirring and homogenizing at 4500r/min, homogenizing at 75 ℃ for 8 minutes, then preserving the temperature for 15 minutes, cooling to room temperature of 25 ℃, adding 1 weight part of hyaluronic acid quaternary ammonium salt and 3 weight parts of growth factors, stirring at the rotating speed of 300r/min for 10 minutes, and uniformly mixing to obtain the cosmetic containing the growth factors.
The growth factor is stem cell growth factor, is prepared according to the method shown in the example 1 in the Chinese patent with the application number of 201510420174.8, and has the following component analysis results: the content of the stem cell growth factor is 33 wt%, and specifically comprises the following components: the content of epidermal growth factor is 18 wt%, the content of fibroblast growth factor is 8 wt%, the content of platelet-derived growth factor is 2 wt%, and the content of vascular endothelial growth factor is 5 wt%.
The honeysuckle extracting solution is prepared by the following method: pulverizing flos Lonicerae to 16 mesh to obtain flos Lonicerae powder; mixing honeysuckle powder and deionized water according to a mass ratio of 1: 10, mixing, carrying out ultrasonic extraction at 55 ℃ for 3h, wherein the ultrasonic power of the ultrasonic extraction is 400W, the ultrasonic frequency is 35kHz, then centrifuging at the rotating speed of 6000r/min for 30min to remove bottom sediment, and filtering supernate with a ceramic membrane of 0.45 mu m to obtain the honeysuckle extracting solution.
The dipsacus root extract is prepared by the following method: crushing the teasel roots to 16 meshes to obtain teasel root powder; mixing the teasel root powder and 15 wt% of ethanol water solution according to the mass ratio of 1: 10, mixing, carrying out ultrasonic extraction at 50 ℃ for 3h, wherein the ultrasonic power of the ultrasonic extraction is 400W, the ultrasonic frequency is 35kHz, then centrifuging at the rotating speed of 6000r/min for 30min to remove bottom sediment, and filtering supernate with a ceramic membrane of 0.45 mu m to obtain the dipsacus asperoides extract.
Example 3
A method for preparing a cosmetic containing growth factors comprises the following steps:
(1) adding 60 parts by weight of deionized water and 0.1 part by weight of disodium ethylene diamine tetraacetate into a water phase pot of a vacuum homogenizing emulsifying machine, stirring for 5min at the rotating speed of 300r/min, uniformly mixing, then adding 8 parts by weight of glycerol, 2.5 parts by weight of 1, 3-propylene glycol, 1 part by weight of cetostearyl alcohol, 10 parts by weight of honeysuckle extract, 5 parts by weight of dipsacus asperoides extract and 1 part by weight of hydroxyethyl cellulose modifier, heating to 75 ℃, stirring for 15min at the rotating speed of 300r/min, and uniformly mixing to obtain a water phase material;
(2) adding 2 parts by weight of glycerol monostearate, 2 parts by weight of sorbitan monopalmitate and 2.5 parts by weight of shea butter into an oil phase pot of a vacuum homogenizing and emulsifying machine, heating to 75 ℃, stirring at the rotating speed of 300r/min for 15min, and uniformly mixing to obtain an oil phase material;
(3) pumping the water phase material into an emulsifying pot of a vacuum homogenizing emulsifying machine, pumping the oil phase material while stirring and homogenizing at 4500r/min, homogenizing at 75 ℃ for 8 minutes, then preserving the temperature for 15 minutes, cooling to room temperature of 25 ℃, adding 1 weight part of hyaluronic acid quaternary ammonium salt and 3 weight parts of growth factors, stirring at the rotating speed of 300r/min for 10 minutes, and uniformly mixing to obtain the cosmetic containing the growth factors.
The growth factor is stem cell growth factor, is prepared according to the method shown in the example 1 in the Chinese patent with the application number of 201510420174.8, and has the following component analysis results: the content of the stem cell growth factor is 33 wt%, and specifically comprises the following components: the content of epidermal growth factor is 18 wt%, the content of fibroblast growth factor is 8 wt%, the content of platelet-derived growth factor is 2 wt%, and the content of vascular endothelial growth factor is 5 wt%.
The hydroxyethyl cellulose modifier is prepared by the following method: hydroxyethyl cellulose and deionized water are mixed according to the mass ratio of 1: 20 stirring and mixing for 15min at the rotating speed of 300r/min, then adding zinc glycinate accounting for 2 percent of the weight of the hydroxyethyl cellulose and zinc lactate accounting for 2 percent of the weight of the hydroxyethyl cellulose, and stirring for 3h at the rotating speed of 300r/min to obtain a reaction solution; spray drying the reaction solution, wherein the inlet air temperature of the spray drying is 180 ℃, the outlet air temperature is 80 ℃, and the hot air speed is 4m3And/min, the feeding speed is 35mL/min, and the rotating speed of an atomizer is 300r/min, so that the hydroxyethyl cellulose modified substance is obtained.
The honeysuckle extracting solution is prepared by the following method: pulverizing flos Lonicerae to 16 mesh to obtain flos Lonicerae powder; mixing honeysuckle powder and deionized water according to a mass ratio of 1: 10, mixing, carrying out ultrasonic extraction at 55 ℃ for 3h, wherein the ultrasonic power of the ultrasonic extraction is 400W, the ultrasonic frequency is 35kHz, then centrifuging at the rotating speed of 6000r/min for 30min to remove bottom sediment, and filtering supernate with a ceramic membrane of 0.45 mu m to obtain the honeysuckle extracting solution.
The dipsacus root extract is prepared by the following method: crushing the teasel roots to 16 meshes to obtain teasel root powder; mixing the teasel root powder and 15 wt% of ethanol water solution according to the mass ratio of 1: 10, mixing, carrying out ultrasonic extraction at 50 ℃ for 3h, wherein the ultrasonic power of the ultrasonic extraction is 400W, the ultrasonic frequency is 35kHz, then centrifuging at the rotating speed of 6000r/min for 30min to remove bottom sediment, and filtering supernate with a ceramic membrane of 0.45 mu m to obtain the dipsacus asperoides extract.
Example 4
A method for preparing a cosmetic containing growth factors comprises the following steps:
(1) adding 60 parts by weight of deionized water and 0.1 part by weight of disodium ethylene diamine tetraacetate into a water phase pot of a vacuum homogenizing emulsifying machine, stirring for 5min at the rotating speed of 300r/min, uniformly mixing, then adding 8 parts by weight of glycerol, 2.5 parts by weight of 1, 3-propylene glycol, 1 part by weight of cetostearyl alcohol, 10 parts by weight of honeysuckle extract, 5 parts by weight of dipsacus asperoides extract and 1 part by weight of hydroxyethyl cellulose modifier, heating to 75 ℃, stirring for 15min at the rotating speed of 300r/min, and uniformly mixing to obtain a water phase material;
(2) adding 2 parts by weight of glycerol monostearate, 2 parts by weight of sorbitan monopalmitate and 2.5 parts by weight of shea butter into an oil phase pot of a vacuum homogenizing and emulsifying machine, heating to 75 ℃, stirring at the rotating speed of 300r/min for 15min, and uniformly mixing to obtain an oil phase material;
(3) pumping the water phase material into an emulsifying pot of a vacuum homogenizing emulsifying machine, pumping the oil phase material while stirring and homogenizing at 4500r/min, homogenizing at 75 ℃ for 8 minutes, then preserving the temperature for 15 minutes, cooling to 25 ℃ at room temperature, adding 1 weight part of amphiphilic carboxymethyl chitosan octadecyl ammonium chloride and 3 weight parts of growth factors, stirring at the rotating speed of 300r/min for 10 minutes, and uniformly mixing to obtain the cosmetic containing the growth factors.
The growth factor is stem cell growth factor, is prepared according to the method shown in the example 1 in the Chinese patent with the application number of 201510420174.8, and has the following component analysis results: the content of the stem cell growth factor is 33 wt%, and specifically comprises the following components: the content of epidermal growth factor is 18 wt%, the content of fibroblast growth factor is 8 wt%, the content of platelet-derived growth factor is 2 wt%, and the content of vascular endothelial growth factor is 5 wt%.
The hydroxyethyl cellulose modifier is prepared by the following method: hydroxyethyl cellulose and deionized water are mixed according to the mass ratio of 1: 20 stirring at 300r/min for 15min, adding hydroxyethyl cellulose 2% of zinc glycinate, zinc lactate accounting for 2% of the weight of the hydroxyethyl cellulose and catechin accounting for 2% of the weight of the hydroxyethyl cellulose are stirred for 3 hours at the rotating speed of 300r/min to obtain reaction liquid; spray drying the reaction solution, wherein the inlet air temperature of the spray drying is 180 ℃, the outlet air temperature is 80 ℃, and the hot air speed is 4m3And/min, the feeding speed is 35mL/min, and the rotating speed of an atomizer is 300r/min, so that the hydroxyethyl cellulose modified substance is obtained.
The honeysuckle extracting solution is prepared by the following method: pulverizing flos Lonicerae to 16 mesh to obtain flos Lonicerae powder; mixing honeysuckle powder and deionized water according to a mass ratio of 1: 10, mixing, carrying out ultrasonic extraction at 55 ℃ for 3h, wherein the ultrasonic power of the ultrasonic extraction is 400W, the ultrasonic frequency is 35kHz, then centrifuging at the rotating speed of 6000r/min for 30min to remove bottom sediment, and filtering supernate with a ceramic membrane of 0.45 mu m to obtain the honeysuckle extracting solution.
The dipsacus root extract is prepared by the following method: crushing the teasel roots to 16 meshes to obtain teasel root powder; mixing the teasel root powder and 15 wt% of ethanol water solution according to the mass ratio of 1: 10, mixing, carrying out ultrasonic extraction at 50 ℃ for 3h, wherein the ultrasonic power of the ultrasonic extraction is 400W, the ultrasonic frequency is 35kHz, then centrifuging at the rotating speed of 6000r/min for 30min to remove bottom sediment, and filtering supernate with a ceramic membrane of 0.45 mu m to obtain the dipsacus asperoides extract.
Example 5
A method for preparing a cosmetic containing growth factors comprises the following steps:
(1) adding 60 parts by weight of deionized water and 0.1 part by weight of disodium ethylene diamine tetraacetate into a water phase pot of a vacuum homogenizing emulsifying machine, stirring for 5min at the rotating speed of 300r/min, uniformly mixing, then adding 8 parts by weight of glycerol, 2.5 parts by weight of 1, 3-propylene glycol, 1 part by weight of cetostearyl alcohol, 10 parts by weight of honeysuckle extract, 5 parts by weight of dipsacus asperoides extract and 1 part by weight of hydroxyethyl cellulose modifier, heating to 75 ℃, stirring for 15min at the rotating speed of 300r/min, and uniformly mixing to obtain a water phase material;
(2) adding 2 parts by weight of glycerol monostearate, 2 parts by weight of sorbitan monopalmitate and 2.5 parts by weight of shea butter into an oil phase pot of a vacuum homogenizing and emulsifying machine, heating to 75 ℃, stirring at the rotating speed of 300r/min for 15min, and uniformly mixing to obtain an oil phase material;
(3) pumping the water phase material into an emulsifying pot of a vacuum homogenizing emulsifying machine, pumping the oil phase material while stirring and homogenizing at 4500r/min, homogenizing at 75 ℃ for 8 minutes, then preserving the temperature for 15 minutes, cooling to 25 ℃ and adding 0.7 weight part of hyaluronic acid quaternary ammonium salt, 0.3 weight part of amphiphilic carboxymethyl chitosan octadecyl ammonium chloride and 3 weight parts of growth factors, stirring at the rotating speed of 300r/min for 10 minutes and uniformly mixing to obtain the cosmetic containing the growth factors.
The growth factor is stem cell growth factor, is prepared according to the method shown in the example 1 in the Chinese patent with the application number of 201510420174.8, and has the following component analysis results: the content of the stem cell growth factor is 33 wt%, and specifically comprises the following components: the content of epidermal growth factor is 18 wt%, the content of fibroblast growth factor is 8 wt%, the content of platelet-derived growth factor is 2 wt%, and the content of vascular endothelial growth factor is 5 wt%.
The hydroxyethyl cellulose modifier is prepared by the following method: hydroxyethyl cellulose and deionized water are mixed according to the mass ratio of 1: 20 stirring and mixing for 15min at the rotating speed of 300r/min, then adding zinc glycinate accounting for 2 percent of the weight of the hydroxyethyl cellulose, zinc lactate accounting for 2 percent of the weight of the hydroxyethyl cellulose and catechin accounting for 2 percent of the weight of the hydroxyethyl cellulose, and stirring for 3h at the rotating speed of 300r/min to obtain a reaction solution; spray drying the reaction solution, wherein the inlet air temperature of the spray drying is 180 ℃, the outlet air temperature is 80 ℃, and the hot air speed is 4m3And/min, the feeding speed is 35mL/min, and the rotating speed of an atomizer is 300r/min, so that the hydroxyethyl cellulose modified substance is obtained.
The honeysuckle extracting solution is prepared by the following method: pulverizing flos Lonicerae to 16 mesh to obtain flos Lonicerae powder; mixing honeysuckle powder and deionized water according to a mass ratio of 1: 10, mixing, carrying out ultrasonic extraction at 55 ℃ for 3h, wherein the ultrasonic power of the ultrasonic extraction is 400W, the ultrasonic frequency is 35kHz, then centrifuging at the rotating speed of 6000r/min for 30min to remove bottom sediment, and filtering supernate with a ceramic membrane of 0.45 mu m to obtain the honeysuckle extracting solution.
The dipsacus root extract is prepared by the following method: crushing the teasel roots to 16 meshes to obtain teasel root powder; mixing the teasel root powder and 15 wt% of ethanol water solution according to the mass ratio of 1: 10, mixing, carrying out ultrasonic extraction at 50 ℃ for 3h, wherein the ultrasonic power of the ultrasonic extraction is 400W, the ultrasonic frequency is 35kHz, then centrifuging at the rotating speed of 6000r/min for 30min to remove bottom sediment, and filtering supernate with a ceramic membrane of 0.45 mu m to obtain the dipsacus asperoides extract.
Test example 1
The cosmetic properties containing the growth factors of examples 1 to 5 were tested. Specific results are shown in table 1.
And (4) moisture retention performance test: 150 female subjects were randomized into 5 groups of 30 subjects, age: 30-40 years old. Health condition: the skin of the subject is healthy, has no allergic history of the skin disease, and meets the voluntary selection standard of the subject. The test method comprises the following steps: the skin elasticity Cotomerma MPA580 host manufactured by Courage + Khazaka, Germany (CK, Germany) was used, the probe used was a ReviscometerRV600 elastic fiber tissue test probe, and the average was taken five times. Test area: the forehead area 2cm above the middle of the eyebrow, the chin area 2cm below the middle of the lower lip, and the left and right cheek areas at the intersection of the nose tip and the pupil. Measuring blank value (R2) 30min after cleaning tested area, and providing the subject with growth factor-containing cosmetic in an amount of 0.1g/cm2(ii) a The skin elasticity index R2 value of the test area after 2 weeks of testing (the negative pressure during the test was constant at 450 mbar). Specification of indexes: the closer the skin elasticity index R2 is to 1, the better the elasticity of the skin. R2 ═ Ua/Uf. Uf — maximum amount of skin stretch. Ua-the recovery value of the skin from the elimination of the negative pressure to the next successive test of the skin surface plus the negative pressure. The comparative test results of each group show that P is less than 0.05, and the difference has statistical significance.
Measurement of skin complexion: 150 female subjects were divided into 5 groups of 30, age: 30-40 years old, 3X 3cm are drawn on the left and right forearm flexor sides of the subject2The measurement of L value of skin color at target site was carried out using Colorimeter CL400, a skin color tester manufactured by Courage + Khazaka (CK) Co., Germany, and evaluation was carried out by measuring the change in L value of the test area before use and after 30 days of use of the productEffect of the product on skin shine. Δ L-experimental area L value after 30 days of use-experimental area L value before use. The comparison results of all groups show that P is less than 0.05, and the difference has statistical significance.
Table 1: test result table
According to the invention, through a large number of researches, the influence of the additives on the performances of the cosmetics such as stability, skin feeling, air temperature and the like is examined, and whether the functional components in the additives can still exert the functions in the system environment with complex cosmetic components is examined, so that the prepared dipsacus root extract and the honeysuckle extract have excellent moisturizing or antioxidant effects.
Claims (5)
1. A preparation method of a cosmetic containing growth factors is characterized by comprising the following steps:
(1) adding 50-70 parts by weight of water and 0.05-0.15 part by weight of disodium ethylene diamine tetraacetate into a water phase pot, stirring and mixing uniformly, then adding 5-10 parts by weight of glycerol, 1-4 parts by weight of 1, 3-propylene glycol, 0.5-1.5 parts by weight of cetostearyl alcohol, 9-11 parts by weight of honeysuckle extract, 4-6 parts by weight of dipsacus asperoides extract and 0.5-1.5 parts by weight of hydroxyethyl cellulose modifier, heating to 55-85 ℃, stirring and mixing uniformly to obtain a water phase material;
(2) adding 0.5-3 parts by weight of glycerol monostearate, 0.5-3 parts by weight of sorbitan monopalmitate and 1-4 parts by weight of shea butter into an oil phase pot, heating to 55-85 ℃, and uniformly stirring and mixing to obtain an oil phase material;
(3) pumping the water phase material into an emulsifying pot, pumping the oil phase material while stirring and homogenizing, homogenizing at 55-85 deg.C for 3-13 min, keeping the temperature for 10-20min, cooling to room temperature, adding 0.5-1.5 weight parts of moisturizing additive and 1-5 weight parts of growth factor, and mixing well to obtain the final product;
the hydroxyethyl cellulose modifier is prepared by the following method: hydroxyethyl cellulose and water are mixed according to the mass ratio of 1: (10-30) stirring at the rotation speed of 200-; spray drying the reaction solution to obtain the product;
the honeysuckle extracting solution is prepared by the following method: pulverizing flos Lonicerae to 10-20 mesh to obtain flos Lonicerae powder; mixing honeysuckle powder and water according to a mass ratio of 1: (5-15), ultrasonically extracting for 2-5h at the temperature of 45-65 ℃, centrifuging to remove bottom sediment, and filtering supernate with a ceramic membrane of 0.1-0.5 mu m to obtain the product;
the dipsacus root extract is prepared by the following method: pulverizing radix Dipsaci to 10-20 mesh to obtain radix Dipsaci powder; mixing the teasel root powder and 10-20wt% of ethanol water solution according to the mass ratio of 1: (5-15), ultrasonically extracting for 2-5h at the temperature of 45-65 ℃, centrifuging to remove bottom sediment, and filtering supernate with a ceramic membrane of 0.1-0.5 mu m to obtain the product;
the moisturizing additive consists of 25-35wt% of amphiphilic carboxymethyl chitosan octadecyl ammonium chloride and 65-75wt% of hyaluronic acid quaternary ammonium salt.
2. The method for preparing a cosmetic containing a growth factor according to claim 1, wherein: the growth factor is a stem cell growth factor.
3. The method for preparing a cosmetic containing a growth factor according to claim 1, wherein: the inlet air temperature of the spray drying is 170-190 ℃, the outlet air temperature is 70-90 ℃, and the hot air speed is 2-5m3The material feeding speed is 25-45mL/min, and the rotating speed of the atomizer is 200-500 r/min.
4. The method for preparing a cosmetic containing a growth factor according to claim 1, wherein: the ultrasonic power of the ultrasonic extraction is 300-.
5. A growth factor-containing cosmetic which is characterized by being produced by the production method of the growth factor-containing cosmetic according to any one of claims 1 to 4.
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