CN109549862A - Cosmetics and preparation method thereof containing growth factor - Google Patents
Cosmetics and preparation method thereof containing growth factor Download PDFInfo
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- CN109549862A CN109549862A CN201910089050.4A CN201910089050A CN109549862A CN 109549862 A CN109549862 A CN 109549862A CN 201910089050 A CN201910089050 A CN 201910089050A CN 109549862 A CN109549862 A CN 109549862A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
Abstract
The invention discloses a kind of cosmetics and preparation method thereof containing growth factor, the preparation method comprises the following steps: (1) water, disodium ethylene diamine tetraacetate being added in water phase pot and be uniformly mixed, then glycerol, 1,3-PD, cetostearyl alcohol, plant extraction liquid, hydroxyethyl cellulose is added to be uniformly mixed；(2) glyceryl monostearate, sorbitan monopalmitate, shea butter are added in oily phase pot and are uniformly mixed；(3) homogeneous.Cosmetics and preparation method thereof of the present invention containing growth factor, it is safe and non-stimulating, have both nutrition and moisten effect, with nutrition, anti-aging, stable emulsion, antibacterial anti-inflammatory, promote the specific functions such as wound healing and reparation damaged cell, also have the effect of efficient moisture-retention, lasting water lock, the moisture content for keeping keratoderma can be continued, guarantee the moist degree of skin, make skin-tightening, rich in elasticity, gloss, commonly using can make skin it is finer and smoother, it is soft and smooth and full of elasticity.
Description
Technical field
The present invention relates to cosmetic technical fields, and in particular to a kind of cosmetics and preparation method thereof containing growth factor.
Background technique
Cosmetics are to impose on any position of human body surface (skin, nail, mouth with smearing, sprinkling or other similar method
Lip, the ripe film in oral cavity etc.), to reach cleaning, eliminate bad smell, skin care, beauty and the product for modifying purpose.
Sebum be by skin inside sebocyte cell generate, be then secreted into skin surface skin oil.It is demonstrate,proved through research
The composition of bright sebum is approximately as about 57% triglycerides or fatty acid, about 12% squalene, about 3% sterol ester, about
23% wax ester and the cholesterol of 1-2%.
For a long time, dermatology expert, cosmetics expert can effectively maintain human skin striving to find, or be
Treat certain dermopathic all kinds of natural materials or artificial synthesized substance.Now, cosmetics had developed to one it is relatively numerous
In flourish period, various forms, the cosmetics of various functions, skin care item are tinkling of jades meets the eye on every side.In numerous skin care item, contain natural nothing
Stimulation component, the product for really being able to the formula of effective skin care ingredient is always skin care item manufacturing enterprise and skin care item consumption
What person was pursued.And the skin care item that can actually meet this requirement often become expensive luxury goods, cannot for as
The consumer objects of majority.
Moisture is one of one of important substance that human body is depended on for existence and important component of human body, lacks moisture human body
The vital movements such as metabolism be subject to influence, such as human body dehydration 10% will threaten health, dehydration 20%
It will causing death.Vitals one of of the skin as human body, water shortage influence it and injure to be also that cannot be neglected.Skin
Being covered in human body surface is influenced maximum by extraneous factors such as environment, is easily dried out, and be will lead to skin after skin water shortage and is lost
Elasticity and reparative regeneration speed slow down the skin problems such as shrivelled, aged, poor flexibility occur, therefore the replenishing water and preserving moisture of skin is repaired
Become a theme of modern cosmetics again.
In addition, in recent years since cosmetics circle are advocated innate efficacy ingredient, by the day with moisturizing or antioxidation
Right plant extracts be added in all kinds of skin protection cosmetics to can anti aging effect be current anti-aging cosmetics a weight
Want research direction.
Summary of the invention
The invention mainly solves the technical problem of providing a kind of cosmetics and preparation method thereof containing growth factor are tools
There are the cosmetics of long-acting holding skin moisturizing, whitening and antibacterial, antioxidant effect.
In order to solve the above technical problems, the technical solution adopted by the present invention is that:
A kind of preparation method of the cosmetics containing growth factor, comprising the following steps:
(1) water, disodium ethylene diamine tetraacetate are added in water phase pot and are uniformly mixed, glycerol, 1,3- third is then added
Glycol, cetostearyl alcohol, plant extraction liquid, hydroxyethyl cellulose are uniformly mixed, and obtain water phase material；
(2) glyceryl monostearate, sorbitan monopalmitate, shea butter are added in oily phase pot and are stirred
It is even, obtain oily mutually material；
(3) will water phase material be pumped into emulsion pot in, stir homogeneous under be pumped into oil mutually expect, homogeneous, be eventually adding moisturizing additive,
Growth factor is uniformly mixed to obtain the final product.
A kind of preparation method of the cosmetics containing growth factor, comprising the following steps:
(1) stirring in water phase pot is added in 50-70 parts by weight water, 0.05-0.15 parts by wt EDTA-disodium to mix
It closes uniformly, 5-10 parts by weight of glycerin, 1-4 parts by weight 1,3-PD, 0.5-1.5 parts by weight cetostearyl alcohol, 9- is then added
11 parts by weight Flos Lonicerae extractive solutions, 4-6 parts by weight teasel extracting solution, the hydroxyethyl cellulose modified object liter of 0.5-1.5 parts by weight
Temperature is uniformly mixed to 55-85 DEG C, obtains water phase material；
(2) by 0.5-3 parts by weight glycerol monostearate, 0.5-3 parts by weight sorbitan monopalmitate, 1-4 parts by weight
Shea butter is added in oily phase pot, is heated to 55-85 DEG C, is uniformly mixed, and obtains oily mutually material；
(3) water phase material is pumped into emulsion pot, stirs suction oil under homogeneous and mutually expects, homogeneous 3-13 minutes at 55-85 DEG C,
Then 10-20min is kept the temperature, is cooled to room temperature and 0.5-1.5 parts by weight moisturizing additive, the mixing of 1-5 parts by weight growth factor is added
Uniformly to obtain the final product.
The growth factor is stem cell factor.The stem cell factor includes epidermal growth factor, fiber
Porcine HGF, platelet derived growth factor and vascular endothelial growth factor.
The hydroxyethyl cellulose modified object is prepared by the following method: be in mass ratio 1 by hydroxyethyl cellulose, water:
(10-30) is that 200-500r/min stirs 10-20min mixing with revolving speed, and the sweet of hydroxyethyl cellulose weight 1-5% is then added
Propylhomoserin zinc, the zinc lactate of hydroxyethyl cellulose weight 1-5%, hydroxyethyl cellulose weight 1-5% catechin be with revolving speed
200-500r/min stirs 2-5h, obtains reaction solution；By reaction solution be spray-dried to get.
The inlet air temperature of the spray drying is 170-190 DEG C, and leaving air temp is 70-90 DEG C, and hot wind wind speed is 2-5m3/
Min, feeding speed 25-45mL/min, atomizer revolving speed are 200-500r/min.
The Flos Lonicerae extractive solution is prepared by the following method: honeysuckle powder being broken to 10-20 mesh, obtains honeysuckle powder；It will
Honeysuckle powder is 1:(5-15 with water in mass ratio) it mixes, it is 45-65 DEG C of ultrasonic extraction 2-5h in temperature, centrifugation removal bottom is heavy
Form sediment, supernatant with 0.1-0.5 μm of ceramic membrane filter to get.
The teasel extracting solution is prepared by the following method: teasel being crushed to 10-20 mesh, obtains teasel powder；It will
Teasel powder is 1:(5-15 with 10-20wt% ethanol water in mass ratio) it mixes, it is 45-65 DEG C of ultrasonic extraction 2- in temperature
5h, centrifugation removal bottom precipitation, supernatant with 0.1-0.5 μm of ceramic membrane filter to get.
The ultrasonic power of the ultrasonic extraction is 300-500W, supersonic frequency 30-45kHz.
The moisturizing additive be amphiphilic carboxymethyl chitosan octadecyl ammonium chloride, in hyaluronic acid quaternary ammonium salt extremely
Few one kind.Further, the moisturizing additive by the amphiphilic carboxymethyl chitosan octadecyl ammonium chloride of 25-35wt% and
65-75wt% hyaluronic acid quaternary ammonium salt composition.
The present invention also provides a kind of cosmetics containing growth factor, by the preparation side of the above-mentioned cosmetics containing growth factor
Method is prepared.
The beneficial effects of the present invention are:
Flos Lonicerae extractive solution of the present invention, extract from Caprifoliaceae woodbine honeysuckle and congener dry flower and/or
The flower that band is just opened, main component are chlorogenic acid (chlorogenic acid) and luteolin (Luteolin), are usually implemented as
Drug, health care product, cosmetics raw material, have anti-inflammatory, antibacterial action.
Teasel extracting solution of the present invention, there is very strong moisture holding capacity, moisture and humidity needed for capable of effectively supplementing skin,
Good skin sense and gloss are allowed the skin to.Teasel also known as teasel root, shaven head, chervil, radix dipsaci, main product Sichuan Province, Hubei
The ground such as province, Hunan Province, Guizhou Province.
Epithelical cell growth factor of the present invention can promote epidermal growth, repair epidermis；Fibroblast growth factor
It can promote fibroblastic growth, collagen is accelerated to generate.
Cosmetics of the present invention containing growth factor, it is safe and non-stimulating, nutrition and moisten effect are had both, there is nutrition, anti-ageing
Always, stable emulsion, antibacterial anti-inflammatory, promote wound healing and repair the specific functions such as damaged cell, also there is efficient moisture-retention, persistently
The effect of water lock can continue the moisture content for keeping keratoderma, guarantee the moist degree of skin, make skin-tightening, be rich in
Elasticity, gloss, commonly using can make skin it is finer and smoother, it is soft and smooth and full of elasticity.
Specific embodiment
Present invention will now be described in detail, and the features and advantages of the invention will become more with these explanations
It is clear, clear.
It is as follows using disclosure: glycerol, No. CAS: 56-81-5.1,3-PD, No. CAS: 504-63-2.Newborn wood fruit
Oil is provided by Xi'an four seasons Biotechnology Co., Ltd.Hydroxyethyl cellulose is provided by Jinan Yu Yi Chemical Co., Ltd., product
Grade is top grade product.Glycine zine, No. CAS: 7214-08-6.Zinc lactate, i.e. Pfansteihl zinc, No. CAS: 103404-76-8.River
Teasel root is provided by hundred herbal medicine industry of Bozhou City Huimin sale Co., Ltd, and source area is Sichuan.Honeysuckle by Bozhou City Sai Er it is great in
Medicinal material is sold Co., Ltd and is provided, and source area is Chongqing.Disodium ethylene diamine tetraacetate, No. CAS: 139-33-3.Glycerol list is stearic
Acid esters, No. CAS: 31566-31-1.Cetostearyl alcohol, No. CAS: 67762-27-0.Sorbitan monopalmitate, No. CAS:
26266-57-9.Catechin, No. CAS: 7295-85-4.Amphiphilic carboxymethyl chitosan octadecyl ammonium chloride is according to application number
For the preparation of method shown in embodiment 1 in 200710056993.4 Chinese patent.Hyaluronic acid quaternary ammonium salt according to application No. is
200810016722.0 Chinese patent in the preparation of method shown in embodiment 1.Vacuum homogeneous emulsifying machine is applied electromechanics for Shanghai inscription and is set
The vacuum homogeneous emulsifying machine for the model MSE-E that standby Co., Ltd provides, specification 50L.
Embodiment 1
The preparation method of cosmetics containing growth factor, comprising the following steps:
(1) 60 parts by weight of deionized water, 0.1 parts by wt EDTA-disodium are added to the water of vacuum homogeneous emulsifying machine
With revolving speed it is that 300r/min stirs 5min and is uniformly mixed in phase pot, is then added 8 parts by weight of glycerin, 2.5 parts by weight 1,3- the third two
Alcohol, 1 parts by weight cetostearyl alcohol, 10 parts by weight Flos Lonicerae extractive solutions, 5 parts by weight teasel extracting solutions, 1 parts by weight ethoxy are fine
Tieing up plain modifier and being warming up to 75 DEG C with revolving speed is that 300r/min stirring 15min is uniformly mixed, and obtains water phase material；
(2) by 2 parts by weight glycerol monostearate, 2 parts by weight sorbitan monopalmitates, 2.5 parts by weight shea butters
It is added in the oily phase pot of vacuum homogeneous emulsifying machine, is heated to 75 DEG C, be that 300r/min stirring 15min is uniformly mixed with revolving speed, obtain
Mutually expect to oily；
(3) water phase material is pumped into the emulsion pot of vacuum homogeneous emulsifying machine, is pumped into oily phase in the case where 4500r/min stirs homogeneous
Material, homogeneous 8 minutes, then keep the temperature 15min at 75 DEG C, and 1 parts by weight hyaluronic acid quaternary ammonium salt, 3 are added after being down to 25 DEG C of room temperature
Parts by weight growth factor is that 300r/min stirs the uniformly mixed cosmetics containing growth factor to obtain the final product of 10min with revolving speed.
The growth factor is stem cell factor, according to real in the Chinese patent application No. is 201510420174.8
Apply the preparation of method shown in example 1, composition analysis result are as follows: stem cell factor content is 33wt%, specifically: epidermal growth factor
Sub- content 18wt%, fibroblast growth factor content 8wt%, platelet derived growth factor content 2wt%, blood vessel endothelium are raw
Long factor content 5wt%.
The hydroxyethyl cellulose modified object is prepared by the following method: in mass ratio by hydroxyethyl cellulose, deionized water
For 1:20 with revolving speed be 300r/min stir 15min mixing, then be added hydroxyethyl cellulose weight 2% glycine zine, hydroxyl
The zinc lactate of ethyl cellulose weight 2%, the catechin of hydroxyethyl cellulose weight 2% stir 3h with revolving speed for 300r/min,
Obtain reaction solution；Reaction solution is spray-dried, the inlet air temperature of the spray drying is 180 DEG C, and leaving air temp is 80 DEG C, hot wind
Wind speed is 4m3/ min, feeding speed 35mL/min, atomizer revolving speed are 300r/min to get hydroxyethyl cellulose modified object.
The Flos Lonicerae extractive solution is prepared by the following method: honeysuckle powder being broken to 16 mesh, obtains honeysuckle powder；By gold and silver
Pollen is that 1:10 is mixed with deionized water in mass ratio, is 55 DEG C of ultrasonic extraction 3h, the ultrasonic function of the ultrasonic extraction in temperature
Rate is 400W, supersonic frequency 35kHz, is then that 6000r/min is centrifuged 30min and removes bottom precipitation with revolving speed, supernatant is used
0.45 μm of ceramic membrane filter is to get Flos Lonicerae extractive solution.
The teasel extracting solution is prepared by the following method: teasel being crushed to 16 mesh, obtains teasel powder；River is continued
Disconnected powder is that 1:10 is mixed with 15wt% ethanol water in mass ratio, is 50 DEG C of ultrasonic extraction 3h, the ultrasonic extraction in temperature
Ultrasonic power be 400W, supersonic frequency 35kHz, then with revolving speed be 6000r/min be centrifuged 30min remove bottom precipitation,
Supernatant is with 0.45 μm of ceramic membrane filter to get teasel extracting solution.
Embodiment 2
The preparation method of cosmetics containing growth factor, comprising the following steps:
(1) 60 parts by weight of deionized water, 0.1 parts by wt EDTA-disodium are added to the water of vacuum homogeneous emulsifying machine
With revolving speed it is that 300r/min stirs 5min and is uniformly mixed in phase pot, is then added 8 parts by weight of glycerin, 2.5 parts by weight 1,3- the third two
Alcohol, 1 parts by weight cetostearyl alcohol, 10 parts by weight Flos Lonicerae extractive solutions, 5 parts by weight teasel extracting solutions, 1 parts by weight ethoxy are fine
It is that 300r/min stirring 15min is uniformly mixed that dimension element, which is warming up to 75 DEG C with revolving speed, obtains water phase material；
(2) by 2 parts by weight glycerol monostearate, 2 parts by weight sorbitan monopalmitates, 2.5 parts by weight shea butters
It is added in the oily phase pot of vacuum homogeneous emulsifying machine, is heated to 75 DEG C, be that 300r/min stirring 15min is uniformly mixed with revolving speed, obtain
Mutually expect to oily；
(3) water phase material is pumped into the emulsion pot of vacuum homogeneous emulsifying machine, is pumped into oily phase in the case where 4500r/min stirs homogeneous
Material, homogeneous 8 minutes, then keep the temperature 15min at 75 DEG C, and 1 parts by weight hyaluronic acid quaternary ammonium salt, 3 are added after being down to 25 DEG C of room temperature
Parts by weight growth factor is that 300r/min stirs the uniformly mixed cosmetics containing growth factor to obtain the final product of 10min with revolving speed.
The growth factor is stem cell factor, according to real in the Chinese patent application No. is 201510420174.8
Apply the preparation of method shown in example 1, composition analysis result are as follows: stem cell factor content is 33wt%, specifically: epidermal growth factor
Sub- content 18wt%, fibroblast growth factor content 8wt%, platelet derived growth factor content 2wt%, blood vessel endothelium are raw
Long factor content 5wt%.
The Flos Lonicerae extractive solution is prepared by the following method: honeysuckle powder being broken to 16 mesh, obtains honeysuckle powder；By gold and silver
Pollen is that 1:10 is mixed with deionized water in mass ratio, is 55 DEG C of ultrasonic extraction 3h, the ultrasonic function of the ultrasonic extraction in temperature
Rate is 400W, supersonic frequency 35kHz, is then that 6000r/min is centrifuged 30min and removes bottom precipitation with revolving speed, supernatant is used
0.45 μm of ceramic membrane filter is to get Flos Lonicerae extractive solution.
The teasel extracting solution is prepared by the following method: teasel being crushed to 16 mesh, obtains teasel powder；River is continued
Disconnected powder is that 1:10 is mixed with 15wt% ethanol water in mass ratio, is 50 DEG C of ultrasonic extraction 3h, the ultrasonic extraction in temperature
Ultrasonic power be 400W, supersonic frequency 35kHz, then with revolving speed be 6000r/min be centrifuged 30min remove bottom precipitation,
Supernatant is with 0.45 μm of ceramic membrane filter to get teasel extracting solution.
Embodiment 3
The preparation method of cosmetics containing growth factor, comprising the following steps:
(1) 60 parts by weight of deionized water, 0.1 parts by wt EDTA-disodium are added to the water of vacuum homogeneous emulsifying machine
With revolving speed it is that 300r/min stirs 5min and is uniformly mixed in phase pot, is then added 8 parts by weight of glycerin, 2.5 parts by weight 1,3- the third two
Alcohol, 1 parts by weight cetostearyl alcohol, 10 parts by weight Flos Lonicerae extractive solutions, 5 parts by weight teasel extracting solutions, 1 parts by weight ethoxy are fine
Tieing up plain modifier and being warming up to 75 DEG C with revolving speed is that 300r/min stirring 15min is uniformly mixed, and obtains water phase material；
(2) by 2 parts by weight glycerol monostearate, 2 parts by weight sorbitan monopalmitates, 2.5 parts by weight shea butters
It is added in the oily phase pot of vacuum homogeneous emulsifying machine, is heated to 75 DEG C, be that 300r/min stirring 15min is uniformly mixed with revolving speed, obtain
Mutually expect to oily；
(3) water phase material is pumped into the emulsion pot of vacuum homogeneous emulsifying machine, is pumped into oily phase in the case where 4500r/min stirs homogeneous
Material, homogeneous 8 minutes, then keep the temperature 15min at 75 DEG C, and 1 parts by weight hyaluronic acid quaternary ammonium salt, 3 are added after being down to 25 DEG C of room temperature
Parts by weight growth factor is that 300r/min stirs the uniformly mixed cosmetics containing growth factor to obtain the final product of 10min with revolving speed.
The growth factor is stem cell factor, according to real in the Chinese patent application No. is 201510420174.8
Apply the preparation of method shown in example 1, composition analysis result are as follows: stem cell factor content is 33wt%, specifically: epidermal growth factor
Sub- content 18wt%, fibroblast growth factor content 8wt%, platelet derived growth factor content 2wt%, blood vessel endothelium are raw
Long factor content 5wt%.
The hydroxyethyl cellulose modified object is prepared by the following method: in mass ratio by hydroxyethyl cellulose, deionized water
For 1:20 with revolving speed be 300r/min stir 15min mixing, then be added hydroxyethyl cellulose weight 2% glycine zine, hydroxyl
The zinc lactate of ethyl cellulose weight 2% is that 300r/min stirs 3h with revolving speed, obtains reaction solution；Reaction solution is spray-dried,
The inlet air temperature of the spray drying is 180 DEG C, and leaving air temp is 80 DEG C, and hot wind wind speed is 4m3/ min, feeding speed are
35mL/min, atomizer revolving speed are 300r/min to get hydroxyethyl cellulose modified object.
The Flos Lonicerae extractive solution is prepared by the following method: honeysuckle powder being broken to 16 mesh, obtains honeysuckle powder；By gold and silver
Pollen is that 1:10 is mixed with deionized water in mass ratio, is 55 DEG C of ultrasonic extraction 3h, the ultrasonic function of the ultrasonic extraction in temperature
Rate is 400W, supersonic frequency 35kHz, is then that 6000r/min is centrifuged 30min and removes bottom precipitation with revolving speed, supernatant is used
0.45 μm of ceramic membrane filter is to get Flos Lonicerae extractive solution.
The teasel extracting solution is prepared by the following method: teasel being crushed to 16 mesh, obtains teasel powder；River is continued
Disconnected powder is that 1:10 is mixed with 15wt% ethanol water in mass ratio, is 50 DEG C of ultrasonic extraction 3h, the ultrasonic extraction in temperature
Ultrasonic power be 400W, supersonic frequency 35kHz, then with revolving speed be 6000r/min be centrifuged 30min remove bottom precipitation,
Supernatant is with 0.45 μm of ceramic membrane filter to get teasel extracting solution.
Embodiment 4
The preparation method of cosmetics containing growth factor, comprising the following steps:
(1) 60 parts by weight of deionized water, 0.1 parts by wt EDTA-disodium are added to the water of vacuum homogeneous emulsifying machine
With revolving speed it is that 300r/min stirs 5min and is uniformly mixed in phase pot, is then added 8 parts by weight of glycerin, 2.5 parts by weight 1,3- the third two
Alcohol, 1 parts by weight cetostearyl alcohol, 10 parts by weight Flos Lonicerae extractive solutions, 5 parts by weight teasel extracting solutions, 1 parts by weight ethoxy are fine
Tieing up plain modifier and being warming up to 75 DEG C with revolving speed is that 300r/min stirring 15min is uniformly mixed, and obtains water phase material；
(2) by 2 parts by weight glycerol monostearate, 2 parts by weight sorbitan monopalmitates, 2.5 parts by weight shea butters
It is added in the oily phase pot of vacuum homogeneous emulsifying machine, is heated to 75 DEG C, be that 300r/min stirring 15min is uniformly mixed with revolving speed, obtain
Mutually expect to oily；
(3) water phase material is pumped into the emulsion pot of vacuum homogeneous emulsifying machine, is pumped into oily phase in the case where 4500r/min stirs homogeneous
Material, homogeneous 8 minutes, then keep the temperature 15min at 75 DEG C, and the amphiphilic carboxymethyl chitosan of 1 parts by weight is added after being down to 25 DEG C of room temperature
Sugared octadecyl ammonium chloride, 3 parts by weight growth factors with revolving speed be 300r/min stir 10min be uniformly mixed up to containing growth because
The cosmetics of son.
The growth factor is stem cell factor, according to real in the Chinese patent application No. is 201510420174.8
Apply the preparation of method shown in example 1, composition analysis result are as follows: stem cell factor content is 33wt%, specifically: epidermal growth factor
Sub- content 18wt%, fibroblast growth factor content 8wt%, platelet derived growth factor content 2wt%, blood vessel endothelium are raw
Long factor content 5wt%.
The hydroxyethyl cellulose modified object is prepared by the following method: in mass ratio by hydroxyethyl cellulose, deionized water
For 1:20 with revolving speed be 300r/min stir 15min mixing, then be added hydroxyethyl cellulose weight 2% glycine zine, hydroxyl
The zinc lactate of ethyl cellulose weight 2%, the catechin of hydroxyethyl cellulose weight 2% stir 3h with revolving speed for 300r/min,
Obtain reaction solution；Reaction solution is spray-dried, the inlet air temperature of the spray drying is 180 DEG C, and leaving air temp is 80 DEG C, hot wind
Wind speed is 4m3/ min, feeding speed 35mL/min, atomizer revolving speed are 300r/min to get hydroxyethyl cellulose modified object.
The Flos Lonicerae extractive solution is prepared by the following method: honeysuckle powder being broken to 16 mesh, obtains honeysuckle powder；By gold and silver
Pollen is that 1:10 is mixed with deionized water in mass ratio, is 55 DEG C of ultrasonic extraction 3h, the ultrasonic function of the ultrasonic extraction in temperature
Rate is 400W, supersonic frequency 35kHz, is then that 6000r/min is centrifuged 30min and removes bottom precipitation with revolving speed, supernatant is used
0.45 μm of ceramic membrane filter is to get Flos Lonicerae extractive solution.
The teasel extracting solution is prepared by the following method: teasel being crushed to 16 mesh, obtains teasel powder；River is continued
Disconnected powder is that 1:10 is mixed with 15wt% ethanol water in mass ratio, is 50 DEG C of ultrasonic extraction 3h, the ultrasonic extraction in temperature
Ultrasonic power be 400W, supersonic frequency 35kHz, then with revolving speed be 6000r/min be centrifuged 30min remove bottom precipitation,
Supernatant is with 0.45 μm of ceramic membrane filter to get teasel extracting solution.
Embodiment 5
The preparation method of cosmetics containing growth factor, comprising the following steps:
(1) 60 parts by weight of deionized water, 0.1 parts by wt EDTA-disodium are added to the water of vacuum homogeneous emulsifying machine
With revolving speed it is that 300r/min stirs 5min and is uniformly mixed in phase pot, is then added 8 parts by weight of glycerin, 2.5 parts by weight 1,3- the third two
Alcohol, 1 parts by weight cetostearyl alcohol, 10 parts by weight Flos Lonicerae extractive solutions, 5 parts by weight teasel extracting solutions, 1 parts by weight ethoxy are fine
Tieing up plain modifier and being warming up to 75 DEG C with revolving speed is that 300r/min stirring 15min is uniformly mixed, and obtains water phase material；
(2) by 2 parts by weight glycerol monostearate, 2 parts by weight sorbitan monopalmitates, 2.5 parts by weight shea butters
It is added in the oily phase pot of vacuum homogeneous emulsifying machine, is heated to 75 DEG C, be that 300r/min stirring 15min is uniformly mixed with revolving speed, obtain
Mutually expect to oily；
(3) water phase material is pumped into the emulsion pot of vacuum homogeneous emulsifying machine, is pumped into oily phase in the case where 4500r/min stirs homogeneous
Material, homogeneous 8 minutes, then keep the temperature 15min at 75 DEG C, be added after being down to 25 DEG C of room temperature 0.7 parts by weight hyaluronic acid quaternary ammonium salt,
The amphiphilic carboxymethyl chitosan octadecyl ammonium chloride of 0.3 parts by weight, 3 parts by weight growth factors are 300r/min stirring with revolving speed
10min is uniformly mixed up to the cosmetics containing growth factor.
The growth factor is stem cell factor, according to real in the Chinese patent application No. is 201510420174.8
Apply the preparation of method shown in example 1, composition analysis result are as follows: stem cell factor content is 33wt%, specifically: epidermal growth factor
Sub- content 18wt%, fibroblast growth factor content 8wt%, platelet derived growth factor content 2wt%, blood vessel endothelium are raw
Long factor content 5wt%.
The hydroxyethyl cellulose modified object is prepared by the following method: in mass ratio by hydroxyethyl cellulose, deionized water
For 1:20 with revolving speed be 300r/min stir 15min mixing, then be added hydroxyethyl cellulose weight 2% glycine zine, hydroxyl
The zinc lactate of ethyl cellulose weight 2%, the catechin of hydroxyethyl cellulose weight 2% stir 3h with revolving speed for 300r/min,
Obtain reaction solution；Reaction solution is spray-dried, the inlet air temperature of the spray drying is 180 DEG C, and leaving air temp is 80 DEG C, hot wind
Wind speed is 4m3/ min, feeding speed 35mL/min, atomizer revolving speed are 300r/min to get hydroxyethyl cellulose modified object.
The Flos Lonicerae extractive solution is prepared by the following method: honeysuckle powder being broken to 16 mesh, obtains honeysuckle powder；By gold and silver
Pollen is that 1:10 is mixed with deionized water in mass ratio, is 55 DEG C of ultrasonic extraction 3h, the ultrasonic function of the ultrasonic extraction in temperature
Rate is 400W, supersonic frequency 35kHz, is then that 6000r/min is centrifuged 30min and removes bottom precipitation with revolving speed, supernatant is used
0.45 μm of ceramic membrane filter is to get Flos Lonicerae extractive solution.
The teasel extracting solution is prepared by the following method: teasel being crushed to 16 mesh, obtains teasel powder；River is continued
Disconnected powder is that 1:10 is mixed with 15wt% ethanol water in mass ratio, is 50 DEG C of ultrasonic extraction 3h, the ultrasonic extraction in temperature
Ultrasonic power be 400W, supersonic frequency 35kHz, then with revolving speed be 6000r/min be centrifuged 30min remove bottom precipitation,
Supernatant is with 0.45 μm of ceramic membrane filter to get teasel extracting solution.
Test case 1
The cosmetics performance containing growth factor of embodiment 1-5 is tested.Concrete outcome is shown in Table 1.
Performance of keeping humidity test: being randomly divided into 5 groups for 150 female subjects, and every group 30, subject age: 30-40
Year.Health status: subjects skin's health, no skin disease allergies meet subject and volunteer inclusion criteria.Test method: it adopts
The skin elasticity CotometerMPA580 host manufactured with German Courage+Khazaka company (German CK company), is used
Probe be ReviscometerRV600 elastic fibrous tissue test probe, survey and be averaged for five times.Test zone: place between the eyebrows is just
Intermediate up 2cm forehead region, lower lip middle left and right cheek area at 2cm chin area, nose and pupil intersection down
Domain.The blank value (R2) of 30min after being tested region clean is first measured, then regulation subject adheres to sooner or later daily using containing life
The cosmetics of the long factor, dosage 0.1g/cm2；Test zone skin elasticity index R2 value (is born in test process after testing 2 weeks
It presses constant for 450mbar).Index explanation: for skin elasticity index R2 closer to 1, the elasticity of skin is better.R2=Ua/Uf.
Uf --- skin maximum tension amount.Ua --- from negative pressure is cancelled to skin when follow-on test skin surface adds negative pressure again next time
Recovery value.Each group compares test result and shows P < 0.05, and difference has statistical significance.
The measurement of skin complexion: being divided into 5 groups for 150 female subjects, and every group 30, the age: 30-40 years old, tested
Person or so forearm bends side and marks 3 × 3cm2The square Experimental Area of size, it is every square centimeter uniformly smear it is every square centimeter
Even smearing preserving moisture and protecting skin frost 0.1g, the skin color tester produced using German Courage+Khazaka (CK) company
Colorimeter CL400 measures the measurement of target area skin complexion L value, by measurement product before and using real after 30 days
The variation of region L value is tested to assess influence of the product to the bright degree of skin.Experimental Area L value-use after Δ L=is used 30 days
Preceding Experimental Area L value.Each group comparison result shows P < 0.05, and difference has statistical significance.
Table 1: test result table
The present invention by numerous studies, investigated additive to cosmetics performance such as stability itself, using skin sense, temperature
Etc. influence and additive in effect ingredient whether remain to play its function in the system environment of cosmetic composition complexity
Effect, teasel extracting solution, the Flos Lonicerae extractive solution of preparation have excellent moisturizing or antioxidation.
Claims (10)
1. a kind of preparation method of the cosmetics containing growth factor, which comprises the following steps:
(1) will water, disodium ethylene diamine tetraacetate be added water phase pot in be uniformly mixed, then be added glycerol, 1,3-PD,
Cetostearyl alcohol, plant extraction liquid, hydroxyethyl cellulose are uniformly mixed, and obtain water phase material；
(2) glyceryl monostearate, sorbitan monopalmitate, shea butter are added in oily phase pot and are uniformly mixed, obtained
Mutually expect to oily；
(3) water phase material is pumped into emulsion pot, stirs suction oil under homogeneous and mutually expect, homogeneous is eventually adding moisturizing additive, growth
The factor is uniformly mixed to obtain the final product.
2. a kind of preparation method of the cosmetics containing growth factor, which comprises the following steps:
(1) 50-70 parts by weight water, 0.05-0.15 parts by wt EDTA-disodium are added in water phase pot and are stirred
It is even, 5-10 parts by weight of glycerin, 1-4 parts by weight 1,3-PD, 0.5-1.5 parts by weight cetostearyl alcohol, 9-11 weight is then added
Amount part Flos Lonicerae extractive solution, 4-6 parts by weight teasel extracting solution, the hydroxyethyl cellulose modified object of 0.5-1.5 parts by weight are warming up to
55-85 DEG C is uniformly mixed, and obtains water phase material；
(2) by 0.5-3 parts by weight glycerol monostearate, 0.5-3 parts by weight sorbitan monopalmitate, 1-4 parts by weight cream wood
Fruit oil is added in oily phase pot, is heated to 55-85 DEG C, is uniformly mixed, and obtains oily mutually material；
(3) water phase material is pumped into emulsion pot, stirs suction oil under homogeneous and mutually expect, homogeneous 3-13 minutes at 55-85 DEG C, then
10-20min is kept the temperature, is cooled to room temperature and 0.5-1.5 parts by weight moisturizing additive is added, 1-5 parts by weight growth factor is uniformly mixed
To obtain the final product.
3. the preparation method of the cosmetics containing growth factor as claimed in claim 2, it is characterised in that: the growth factor is dry
Porcine HGF.
4. the preparation method of the cosmetics containing growth factor as claimed in claim 2, it is characterised in that: the hydroxyethyl cellulose
Modifier is prepared by the following method: being in mass ratio 1:(10-30 by hydroxyethyl cellulose, water) with revolving speed for 200-500r/min
10-20min mixing is stirred, glycine zine, the hydroxyethyl cellulose weight 1- of hydroxyethyl cellulose weight 1-5% is then added
5% zinc lactate, the catechin of hydroxyethyl cellulose weight 1-5% stir 2-5h with revolving speed for 200-500r/min, obtain anti-
Answer liquid；By reaction solution be spray-dried to get.
5. the preparation method of the cosmetics containing growth factor as claimed in claim 4, it is characterised in that: the spray drying into
Air temperature is 170-190 DEG C, and leaving air temp is 70-90 DEG C, and hot wind wind speed is 2-5m3/ min, feeding speed 25-45mL/
Min, atomizer revolving speed are 200-500r/min.
6. the preparation method of the cosmetics containing growth factor as claimed in claim 2, it is characterised in that: the Flos Lonicerae extractive solution
It is prepared by the following method: honeysuckle powder being broken to 10-20 mesh, obtains honeysuckle powder；In mass ratio it is 1 by honeysuckle powder and water:
(5-15) mixing is 45-65 DEG C of ultrasonic extraction 2-5h, centrifugation removal bottom precipitation, 0.1-0.5 μm of pottery of supernatant in temperature
Porcelain film filtering to get.
7. the preparation method of the cosmetics containing growth factor as claimed in claim 2, it is characterised in that: the teasel extracting solution
It is prepared by the following method: teasel being crushed to 10-20 mesh, obtains teasel powder；By teasel powder and 10-20wt% ethanol water
Solution is 1:(5-15 in mass ratio) mixing is 45-65 DEG C of ultrasonic extraction 2-5h, centrifugation removal bottom precipitation, supernatant in temperature
Liquid with 0.1-0.5 μm of ceramic membrane filter to get.
8. the preparation method of the cosmetics containing growth factor as claimed in claims 6 or 7, it is characterised in that: the ultrasonic extraction
Ultrasonic power be 300-500W, supersonic frequency 30-45kHz.
9. the preparation method of the cosmetics containing growth factor as claimed in claim 2, it is characterised in that: the moisturizing additive is
At least one of amphiphilic carboxymethyl chitosan octadecyl ammonium chloride, hyaluronic acid quaternary ammonium salt.
10. a kind of cosmetics containing growth factor, which is characterized in that using described in any one of claim 1-9 containing growth because
The preparation method of the cosmetics of son is prepared.
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Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101020725A (en) * | 2007-03-23 | 2007-08-22 | 天津大学 | Amphiphilic chitosan quatermary ammonium salt with long alkane radical and its prepn |
| WO2008134712A2 (en) * | 2007-04-30 | 2008-11-06 | Living Proof, Inc. | Use of matrix metalloproteinase inhibitors in skin care |
| CN101422415A (en) * | 2008-05-30 | 2009-05-06 | 烟台海岸带可持续发展研究所 | Moisture-absorption humectant and preparation method thereof |
| US20100143274A1 (en) * | 2007-03-07 | 2010-06-10 | Ciba Corporation | Use of amines and amides for the stabilization of organic micronized uv absorbers |
| CN104055714A (en) * | 2014-07-08 | 2014-09-24 | 广州千百度化妆品有限公司 | Moisturizing cosmetic composition and preparation method thereof |
| CN104856892A (en) * | 2014-11-14 | 2015-08-26 | 广州远想生物科技有限公司 | Cosmetic or skin care product preparation rich in growth factors and preparation method therefor |
| CN105250428A (en) * | 2015-11-09 | 2016-01-20 | 广州丹奇日用化工厂有限公司 | Plant extract and application thereof |
| WO2016024999A1 (en) * | 2014-08-15 | 2016-02-18 | Nevada Naturals Inc. | Deodorizing and skin cleaning |
| CN105878094A (en) * | 2014-12-05 | 2016-08-24 | 江南大学 | Preparation method of cosmetic containing tea seed antioxidant peptide |
| CN105997771A (en) * | 2016-08-01 | 2016-10-12 | 广州赛莱拉干细胞科技股份有限公司 | Inflammation diminishing and acne removing mask and preparation method thereof |
| WO2017024110A1 (en) * | 2015-08-04 | 2017-02-09 | Isp Investments Llc | Polymers derived from amino-functional vinyl alcohol ethers and applications thereof |
| CN107519034A (en) * | 2017-08-17 | 2017-12-29 | 成都新柯力化工科技有限公司 | A kind of skin deep layer of the bioactie agent containing chelating repairs liquid and preparation method thereof |
| CN107802580A (en) * | 2017-12-08 | 2018-03-16 | 广州赛莱拉干细胞科技股份有限公司 | Essence containing human stem cell growth factor and preparation method thereof |
-
2019
- 2019-01-30 CN CN201910089050.4A patent/CN109549862B/en active Active
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100143274A1 (en) * | 2007-03-07 | 2010-06-10 | Ciba Corporation | Use of amines and amides for the stabilization of organic micronized uv absorbers |
| CN101020725A (en) * | 2007-03-23 | 2007-08-22 | 天津大学 | Amphiphilic chitosan quatermary ammonium salt with long alkane radical and its prepn |
| WO2008134712A2 (en) * | 2007-04-30 | 2008-11-06 | Living Proof, Inc. | Use of matrix metalloproteinase inhibitors in skin care |
| CN101422415A (en) * | 2008-05-30 | 2009-05-06 | 烟台海岸带可持续发展研究所 | Moisture-absorption humectant and preparation method thereof |
| CN104055714A (en) * | 2014-07-08 | 2014-09-24 | 广州千百度化妆品有限公司 | Moisturizing cosmetic composition and preparation method thereof |
| WO2016024999A1 (en) * | 2014-08-15 | 2016-02-18 | Nevada Naturals Inc. | Deodorizing and skin cleaning |
| CN104856892A (en) * | 2014-11-14 | 2015-08-26 | 广州远想生物科技有限公司 | Cosmetic or skin care product preparation rich in growth factors and preparation method therefor |
| CN105878094A (en) * | 2014-12-05 | 2016-08-24 | 江南大学 | Preparation method of cosmetic containing tea seed antioxidant peptide |
| WO2017024110A1 (en) * | 2015-08-04 | 2017-02-09 | Isp Investments Llc | Polymers derived from amino-functional vinyl alcohol ethers and applications thereof |
| CN105250428A (en) * | 2015-11-09 | 2016-01-20 | 广州丹奇日用化工厂有限公司 | Plant extract and application thereof |
| CN105997771A (en) * | 2016-08-01 | 2016-10-12 | 广州赛莱拉干细胞科技股份有限公司 | Inflammation diminishing and acne removing mask and preparation method thereof |
| CN107519034A (en) * | 2017-08-17 | 2017-12-29 | 成都新柯力化工科技有限公司 | A kind of skin deep layer of the bioactie agent containing chelating repairs liquid and preparation method thereof |
| CN107802580A (en) * | 2017-12-08 | 2018-03-16 | 广州赛莱拉干细胞科技股份有限公司 | Essence containing human stem cell growth factor and preparation method thereof |
Non-Patent Citations (5)
| Title |
|---|
| SIONKOWSKA, ALINA等: ""Preparation and characterization of collagen/chitosan/hyaluronic acid thin films for application in hair care cosmetics"", 《PURE AND APPLIED CHEMISTRY》 * |
| 慢生活工坊: "《茶饮与健康》", 31 July 2016, 浙江摄影出版社 * |
| 程乐卿: "《长寿养生经》", 31 January 2016, 青岛出版社 * |
| 谭勇军等: "《精细化工小产品新配方生产与研制》", 30 November 1991, 中南工业大学出版社 * |
| 陈明岭等: "《皮肤病常用中药药理及临床》", 31 October 2017, 中国科学技术出版社 * |
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