CN112245324B - Cosmetic with moisturizing function and preparation method and application thereof - Google Patents
Cosmetic with moisturizing function and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a cosmetic with a moisturizing function, a preparation method and application thereof. The invention researches cactus with excellent water retention function, 2 sections of moisturizing polypeptide obtained by screening through a dry cell model experiment has better moisturizing effect, and the moisturizing polypeptide is selected to be applied to the repair of dry cells.
Description
Technical Field
The invention relates to the field of cosmetics and medicines, in particular to a polypeptide with moisturizing activity and application of the polypeptide in cosmetics prepared by the polypeptide.
Background
The skin is the largest organ of human body, the maintenance of the water content (10% -20%) of the horny layer of healthy skin depends on the structural and functional integrity of the natural moisturizing barrier system of the epidermis, but the functional disorder of the system is often caused by the influence of environmental factors and disease occurrence, such as winter climate dryness, renal failure, application of molecular targeted drugs and the like, the water content of the horny layer is often lower than 10%, the skin is dry, rough, chapped and the like, and even certain skin diseases are induced or aggravated. It is therefore of great importance for skin moisturization, especially for girls, to have a shrapnel breakable skin that is a pursuit of somnolence, which is closely linked to skin moisturization.
The natural moisturizer helps repair skin barrier, supplements lipid in cells, forms a closed environment, improves hydration of stratum corneum cells, reduces inflammation, and reduces microorganisms. The artificial humectant contains more allergic and irritant chemical substances, such as preservatives and perfumes, is easy to cause skin allergy or generate other adverse reactions, and some of the artificial humectant is even carcinogenic and has adverse effects on the next generation. Among natural moisturizers, plant-derived moisturizers have natural advantages of high safety, low acquisition cost and the like, and are the important research direction of moisturizing cosmetics.
Natural moisturizers of vegetable origin comprise many natural vegetable oils such as olive oil, coconut oil, nut oil, aloe vera, polypeptides and the like. The natural vegetable oil can delay the transepidermal water loss, promote the repair of the damaged part and enhance the function of the skin barrier by simulating a natural moisturizing barrier system, thereby maintaining the hydration state of the stratum corneum. Finally, the skin barrier is repaired, and the percutaneous loss water loss (TWEL) is reduced, so that the moisture-keeping effect is achieved. Water is locked mainly by two mechanisms, namely, the hydration of epidermal cells is enhanced by adding hydrophilic components such as glycerol; ② reducing the evaporation of water from the stratum corneum by the occlusive action of hydrophobic ingredients such as petrolatum. There is also evidence that some vegetable oils of hydrophobic composition may diffuse into the intercellular spaces of the stratum corneum to enhance the integrity of the barrier structure. The proportion of each essential fatty acid in natural oil is a major factor affecting the barrier repair benefit. Natural oils with higher concentrations of Linoleic Acid (LA) have better potential for barrier repair than normal natural oils, while excessive levels of irritating Oleic Acid (OA) can adversely impair skin barrier function. It is reported that the proportion of Linoleic Acid (LA) in a natural vegetable oil such as Grape Seed Oil (GSO) in a fatty acid profile exceeds 60%, and thus the natural vegetable oil contributes to the repair of the skin barrier. The cold pressing method is the preferred method for extracting natural vegetable oil, which can retain beneficial grease in a heatless and chemionless process and limit irritant by-products, and the natural vegetable oil extracted by the cold pressing method has the characteristics of oxidation resistance, antibiosis and skin barrier repair. Several studies have preliminarily demonstrated the good role of natural oils in neonatal skin care regimens, on average studies using small doses of oil (2-4 mL/time) massaged to a specified skin area, or applied systemically, 2-4 times/d, helping to protect and improve the integrity of the immature skin barrier. The natural and cold pressed oils are selected for conventional moisturization, increased keratinocyte hydration, and improved skin barrier function. Although topical application of cold-pressed oil appears to be safe, adverse reactions may also include burns, erythema or allergic reactions. It has been reported that vegetable oils may also cause allergic contact dermatitis in some humans, but natural vegetable oils extracted by cold pressing have a lower incidence than essential oils. In general, natural oils have been demonstrated to have anti-inflammatory and anti-itching properties, as well as antimicrobial properties.
The plant active polypeptide is widely applied to various fields of beauty treatment, health care, skin anti-aging and the like, particularly has natural physiological effects of skin whitening, freckle removing, wrinkle resisting, moisture preservation, sebum removal, acne removal and the like, has good oxidation resistance, excessive free radical removal, skin tissue nutrition and skin cell metabolism promotion, can be used as an effective raw material and an ideal additive of the skin beauty and moisture preservation cosmetic, and must make positive progress and pay attention to the application of the skin beauty and moisture preservation cosmetic in the future. However, the research on the use of plant active peptides for moisturizing is still relatively few.
Disclosure of Invention
The invention overcomes the limitation of the prior art, researches the cactus with excellent water retention function, provides the process optimization to obtain the cactus moisturizing polypeptide with five molecular weight sections, and obtains the moisturizing polypeptide with 2 sections through dry cell model experiment screening, thereby having better moisturizing effect.
In one aspect of the invention, a method for separating moisturizing polypeptides from cactus is provided, and the method comprises the following steps of taking cactus powder and n-hexane according to the volume ratio of 1: mixing at a ratio of 3, extracting with shaking in 40 deg.C water bath for 30min, centrifuging at 10000g/min for 3min, discarding supernatant, adding n-hexane again, repeating for 1 time to completely remove fat, placing in a constant temperature drying oven, and oven drying at 65 deg.C to constant weight (4 hr overnight) to obtain powder; adding 10 times of deionized water according to the volume ratio of the powder, adjusting pH to 8.5, adding 0.2% of cellulase and 0.3% of pectinase, hydrolyzing at 50 deg.C for 60min, inactivating enzyme, and cooling to room temperature; adding neutral protease 0.5 wt% of the powder, hydrolyzing at 40 deg.C for 100min, inactivating enzyme, and cooling to room temperature; adding alkaline protease 0.5 wt% of the powder, hydrolyzing at 40 deg.C for 100min, inactivating enzyme, and cooling to room temperature; adding activated carbon 0.5% of the powder weight for decolorizing for half an hour, centrifuging, and collecting filtrate which is light green clear liquid. And (3) filtering the filtrate by a 0.22-micron microporous filter membrane to remove impurities, then respectively filtering the filtrate by hollow fiber membranes with the cut-off amounts of 10kDa, 5kDa and 3kDa and an ultrafiltration membrane with the cut-off amount of 1kDa, and filtering the filtrate by an ultrafiltration membrane with the molecular weight of below 1kDa to remove impurities. And (4) carrying out low-temperature rotary evaporation on the obtained polypeptide with each molecular weight segment, and carrying out cold drying. Five moisturizing polypeptide samples with the molecular weight segments of Mr >10kDa, 5kDa < Mr <10kDa, 3kDa < Mr <5kDa, 1kDa < Mr <3kDa and Mr <1kDa are obtained. Wherein, the two polypeptide groups of 3kDa < Mr <5kDa and Mr <1kDa have the best moisturizing effect,
the invention further provides application of the moisturizing polypeptide in preparing moisturizing cosmetics.
Furthermore, the cosmetic provided by the invention is a skin moistening shower gel, and the preparation method of the skin moistening shower gel comprises the following steps:
(i) adding deionized water into a reaction kettle, heating to 70 ℃, adding decyl glucoside, cocamidopropyl betaine, sodium dodecyl sulfate, glycerol, behenyl alcohol, caprylyl glycol, sodium carboxymethyl cellulose, squalane and L-fucose while stirring at 35 revolutions per minute, and stirring at 100 revolutions per minute for 10 minutes to obtain a mixed solution;
(ii) and cooling the mixed solution to 35 ℃, adding sodium hyaluronate and moisturizing polypeptide, and stirring at 50 revolutions per minute for 20 minutes to obtain the skin-moistening shower gel.
The skin-moistening shower gel comprises the following raw materials in proportion: 2-5% of decyl glucoside, 1-3% of cocamidopropyl betaine, 0.5-1.5% of sodium dodecyl sulfate, 1-5% of glycerol, 0.5-1.5% of behenyl alcohol, 0.5-1.5% of caprylyl glycol, 1-3% of sodium carboxymethyl cellulose, 3-5% of squalane, 1-2% of L-fucose, 0.25-1% of sodium hyaluronate, 1-2% of moisturizing polypeptide and the balance of deionized water.
The invention also provides skin-moistening shower gel which comprises the following raw materials in parts by weight: 2-5% of decyl glucoside, 1-3% of cocamidopropyl betaine, 0.5-1.5% of sodium dodecyl sulfate, 1-5% of glycerol, 0.5-1.5% of behenyl alcohol, 0.5-1.5% of caprylyl glycol, 1-3% of sodium carboxymethyl cellulose, 3-5% of squalane, 1-2% of L-fucose, 0.25-1% of sodium hyaluronate, 1-2% of moisturizing polypeptide and the balance of deionized water.
The invention also provides application of the moisturizing polypeptide in preparation of cosmetics or medicines for repairing skin injury.
The moisturizing polypeptide is prepared by separating cactus and adopting the separation method.
Further, the cosmetic can be face cream, facial mask, facial cleanser, soap, moisturizing water, hand cream and the like.
Furthermore, other moisturizing factors can be added into the cosmetic disclosed by the invention to increase the moisturizing effect. Including but not limited to glycerol.
Furthermore, the cosmetic of the present invention contains a known bactericide.
Advantageous effects
The invention researches cactus with excellent water retention function, provides process optimization to obtain the cactus moisturizing polypeptide with five molecular weight sections, obtains the moisturizing polypeptide with 2 sections through dry cell model experiment screening, has better moisturizing effect, selects the moisturizing polypeptide to be applied to the repair of dry cells, finds that the moisturizing polypeptide has better effect of repairing the dry cells, and also proves that the polypeptide has better moisturizing effect through a mouse model and human body experiments.
Drawings
FIG. 1 graph of the protective effect of moisturizing polypeptides on desiccation damaged HaCaT cells
Detailed Description
To further illustrate the objects, aspects and advantages of the present invention, we shall now describe the invention with reference to the following specific examples, which are only for better illustrating the patent of the present invention and are not intended to limit the scope of the present invention. All other embodiments that can be obtained by a person skilled in the art without making any inventive step based on the examples of the present invention belong to the protection scope of the present invention.
Example 1 preparation of moisturizing polypeptide extract
Taking 1.0kg of cactus powder, and mixing the cactus powder with n-hexane according to the volume ratio of 1: mixing at a ratio of 3, extracting with shaking in 40 deg.C water bath for 30min, centrifuging at 10000g/min for 3min, discarding supernatant, adding n-hexane again, repeating for 1 time to completely remove fat, placing in a constant temperature drying oven, and oven drying at 65 deg.C to constant weight (4 hr overnight) to obtain powder; adding 10 times of deionized water according to the volume ratio of the powder, adjusting pH to 8.5, adding 0.2% of cellulase and 0.3% of pectinase, hydrolyzing at 50 deg.C for 60min, inactivating enzyme, and cooling to room temperature; adding neutral protease 0.5 wt% of the powder, hydrolyzing at 40 deg.C for 100min, inactivating enzyme, and cooling to room temperature; adding alkaline protease 0.5 wt% of the powder, hydrolyzing at 40 deg.C for 100min, inactivating enzyme, and cooling to room temperature; adding activated carbon 0.5% of the powder weight for decolorizing for half an hour, centrifuging, and collecting filtrate which is light green clear liquid. And (3) filtering the filtrate by a 0.22-micron microporous filter membrane to remove impurities, then respectively filtering the filtrate by hollow fiber membranes with the cut-off amounts of 10kDa, 5kDa and 3kDa and an ultrafiltration membrane with the cut-off amount of 1kDa, and filtering the filtrate by an ultrafiltration membrane with the molecular weight of below 1kDa to remove impurities. And (4) carrying out low-temperature rotary evaporation on the obtained polypeptide with each molecular weight segment, and carrying out cold drying. Five moisturizing polypeptide samples with the molecular weight segments of Mr >10kDa, 5kDa < Mr <10kDa, 3kDa < Mr <5kDa, 1kDa < Mr <3kDa and Mr <1kDa are obtained. The results are shown in table 1 below.
TABLE 1 enzymatic moisturizing polypeptide Properties
| Moisturizing polypeptide types | Water content% | Total protein% | Purity% |
| Mr>10kDa | 1.13±0.02 | 98.23±0.79 | 98.31±0.65 |
| 5kDa<Mr<10kDa | 1.39±0.01 | 97.66±1.03 | 97.26±0.44 |
| 3kDa<Mr<5kDa | 2.05±0.04 | 96.87±1.11 | 97.52±0.36 |
| 1kDa<Mr<3kDa | 1.19±0.03 | 95.63±0.73 | 98.02±0.81 |
| Mr<1kDa | 0.96±0.02 | 96.95±0.86 | 97.65±1.03 |
As can be seen from Table 1, the total protein content and purity of the isolated and purified moisturizing polypeptide are higher than those obtained by the conventional extraction method in the field, and are both more than 95%, which is beneficial to the subsequent tests.
Example 2 protective Effect of moisturizing Polypeptides on desiccation-damaged HaCaT cells
The HaCaT cells are positioned on the epidermal layer of the skin, and are recognized in the field to be used for evaluating the effects of moisturizing, relieving inflammation, resisting oxidation and the like. HaCaT cells are inoculated into a 96-well plate and cultured for 24h according to a method known in the art, after the cells are attached to the wall, 100 mu l of 5 moisturizing polypeptide samples prepared in DMEM medium and to be tested and prepared in example 1 is added, after 24h of culture, culture solution in the wells is removed, the 96-well plate is placed in a clean bench and dried for 20min under the condition that the air speed is 0.4m/s (the cell death rate is close to 50%). The sample concentrations in the experiment were 1. mu.g/mL, 10. mu.g/mL, 50. mu.g/mL, 100. mu.g/mL. Wells with DMEM medium alone were used as controls in the experiment. Cell viability was determined. The cell survival rate was calculated as a publication of (%) -cell survival rate (OD450 sample-OD 450 blank)/(OD 450 control-OD 450 blank) x 100%. The results are shown in FIG. 1.
The protective effect of the sample on the dry damaged cells is explored by carrying out damage treatment on the HaCaT cells after sample loading and drying. As shown in FIG. 1, the two polypeptide groups 3kDa < Mr <5kDa and Mr <1kDa can better protect injured cells. The two polypeptide groups can achieve higher effect of resisting cell desiccation injury when the concentration is 50 mu g/mL, and the cell survival rates are respectively 97.3% +/-2.5% and 98.25% +/-2.1%. Therefore, subsequent studies were performed with two polypeptide groups of 3kDa < Mr <5kDa and Mr <1kDa, respectively.
Example 3 Effect of Mr <1kDa Polypeptides on the production of HA in HaCaT cells
The HA ELISA kit is used for determining the HA content in the sample by a double-antibody sandwich method, and the antibody selected in the test is hyaluronic acid. The HA content of HaCaT cells treated by 50 ug/mL Mr <1kDa polypeptide in example 2 was measured. The result shows that the HA content in the HaCaT cells subjected to drying injury is 104.28 +/-3.41 ng/L, while the HA content of the treated cells reaches 226.81 +/-6.02 ng/L, and the HA content is obviously improved.
Example 4 Mr <1kDa polypeptide group Effect on Dry mouse models
70 experimental mice were randomly divided into 7 groups: 1) blank group, injecting normal saline subcutaneously, skin does not smear any product; 2) model group, D-galactose was injected subcutaneously, skin was not smeared with any product; 3) a positive control group, D-galactose is injected subcutaneously, and 10% glycerol solution is smeared on the skin; 4) mr <1kDa low dose group, D-galactose was injected subcutaneously, and the skin application dose was 0.25mg/cm 2; 5) mr <1kDa high dose group, D-galactose was injected subcutaneously, and the skin application dose was 1mg/cm 2. After each group of mice was raised for one week, the experiment was started. All mice were dehaired with 6% sodium sulfide, exposing skin on the back of a 3 × 3cm2 size. The subcutaneous injection dosage is 1000 mg.kg-1. d-1. After 8 weeks of mouse feeding, the experiment was ended. After one day of fasting, the skin was cleaned and the mice were sacrificed by cervical dislocation. And detecting the water content of the removed skin. The results are shown in Table 2.
TABLE 2 Mr <1kDa polypeptide group influence on moisture content of mouse skin
| Group of | Skin moisture content (%) |
| Blank group | 66.48±1.98 |
| Model set | 56.42±2.01## |
| Positive control group | 60.79±1.36* |
| Mr<1kDa Low dose group | 65.13±2.13* |
| Mr<1kDa high dose group | 65.97±1.38** |
# P <0.01 compared to blank; p <0.01 was compared to model groups.
The experimental results show (table 2) that the skin moisture content of the model group mice is significantly lower than that of the blank group (P <0.01), confirming that aging causes the skin moisture content to decrease. Compared with the model group, the moisture content of the skin of the mice of the Mr <1kDa group and the positive control group is obviously improved, the difference has statistical significance (P <0.01), and the moisture content of the skin of the mice of the Mr <1kDa group with high dose has very obvious difference (P <0.01) compared with the model group. Experiments show that the Mr <1kDa peptide has good effect on improving the moisture content of aging skin, wherein the effect of the high-dose Mr <1kDa peptide is most obvious.
Example 5 Mr <1kDa polypeptide human experiments
(1) Preparation of Experimental cosmetics
The experimental cosmetics are skin-moistening shower gel, and the preparation method of the skin-moistening shower gel comprises the following steps:
(i) adding deionized water into a reaction kettle, heating to 70 ℃, adding decyl glucoside, cocamidopropyl betaine, sodium dodecyl sulfate, glycerol, behenyl alcohol, caprylyl glycol, sodium carboxymethyl cellulose, squalane and L-fucose while stirring at 35 revolutions per minute, and stirring at 100 revolutions per minute for 10 minutes to obtain a mixed solution;
(ii) cooling the mixed solution to 35 ℃, adding sodium hyaluronate and Mr <1kDa polypeptide, and stirring at 50 revolutions per minute for 20 minutes to obtain the skin moistening shower gel.
The skin-moistening shower gel comprises the following raw materials in proportion: decyl glucoside 2%, cocamidopropyl betaine 1%, sodium dodecyl sulfate 0.5%, glycerol 1%, behenyl alcohol 0.5%, caprylyl glycol 0.5%, sodium carboxymethyl cellulose 1%, squalane 3%, L-fucose 1%, sodium hyaluronate 0.25%, Mr <1kDa polypeptide 1%, and the balance deionized water.
(2) Preparation of control cosmetic
The control cosmetic is skin-moistening bath cream, and the preparation method of the skin-moistening bath cream comprises the following steps:
(i) adding deionized water into a reaction kettle, heating to 70 ℃, adding decyl glucoside, cocamidopropyl betaine, sodium dodecyl sulfate, glycerol, behenyl alcohol, caprylyl glycol, sodium carboxymethyl cellulose, squalane and L-fucose while stirring at 35 revolutions per minute, and stirring at 100 revolutions per minute for 10 minutes to obtain a mixed solution;
(ii) and cooling the mixed solution to 35 ℃, adding sodium hyaluronate, and stirring at the speed of 50 revolutions per minute for 20 minutes to obtain the skin moistening shower gel.
The skin-moistening shower gel comprises the following raw materials in proportion: decyl glucoside 2%, cocamidopropyl betaine 1%, sodium dodecyl sulfate 0.5%, glycerol 1%, behenyl alcohol 0.5%, caprylyl glycol 0.5%, sodium carboxymethyl cellulose 1%, squalane 3%, L-fucose 1%, sodium hyaluronate 0.25%, and the balance of deionized water.
(3) Control 2 the skin lotion bath cream was a commercially available skin lotion cosmetic.
Selecting 20-40 years old adults, male and female halves, having good health and regular life, having no skin diseases, randomly dividing into 3 groups, wherein each group comprises 20 people, each group uses the skin-moistening bath cream obtained in the embodiment to take a bath, then measuring the moisture content of the neck in an environment with the test environment temperature of 25 ℃ and the relative humidity of 60%, and keeping the testee calm. 2 square experimental areas of 4cm × 4cm are drawn on the back neck, and used as a test area of the skin-moistening shower gel, the skin moisture content 1 hour and 5 hours after bathing is respectively measured by using a skin moisture meter, and the result is recorded. The specific results are shown in Table 3.
Table 3 skin lotion bath lotion moisturizing effect test results table
| Group of | 1h skin moisture content (%) | Skin moisture content (%) 5h |
| Experimental cosmetics | 62.6±2.1 | 59.4±1.0 |
| Control cosmetic | 46.4±1.4 | 40.8±0.9 |
| Control 2 body wash | 52.9±1.5 | 44.2±1.5 |
The results in the table show that the moisturizing performance of the skin-moistening shower gel added with the Mr <1kDa peptide is better than that of the shower gel without the Mr <1kDa peptide, and the effect is better than that of the commercially available skin-moistening shower gel. Moreover, the difference between the water content of 1h and the water content of 5h is not large, which shows that the moisturizing peptide can well improve the water content of the skin and is beneficial to keeping the skin moist.
Claims (4)
1. The preparation method of the skin-moistening shower gel comprises the following steps:
(i) adding deionized water into a reaction kettle, heating to 70 ℃, adding decyl glucoside, cocamidopropyl betaine, sodium dodecyl sulfate, glycerol, behenyl alcohol, caprylyl glycol, sodium carboxymethyl cellulose, squalane and L-fucose while stirring at 35 revolutions per minute, and stirring at 100 revolutions per minute for 10 minutes to obtain a mixed solution;
(ii) cooling the mixed solution to 35 ℃, adding sodium hyaluronate and moisturizing polypeptide, and stirring at 50 revolutions per minute for 20 minutes to obtain the skin-moistening shower gel;
the skin-moistening shower gel comprises the following raw materials in parts by weight: 2-5% of decyl glucoside, 1-3% of cocamidopropyl betaine, 0.5-1.5% of sodium dodecyl sulfate, 1-5% of glycerol, 0.5-1.5% of behenyl alcohol, 0.5-1.5% of caprylyl glycol, 1-3% of sodium carboxymethyl cellulose, 3-5% of squalane, 1-2% of L-fucose, 0.25-1% of sodium hyaluronate, 1-2% of moisturizing polypeptide and the balance of deionized water;
the moisturizing polypeptide is prepared by the following method: the method comprises the following steps of taking cactus powder and n-hexane according to the volume ratio of 1: mixing at a ratio of 3, extracting with shaking in 40 deg.C water bath for 30min, centrifuging at 10000g/min for 3min, discarding supernatant, adding n-hexane again, repeating for 1 time to completely remove fat, placing in a constant temperature drying oven, and drying at 65 deg.C to constant weight to obtain powder; adding 10 times of deionized water according to the volume ratio of the powder, adjusting pH to 8.5, adding 0.2% of cellulase and 0.3% of pectinase, hydrolyzing at 50 deg.C for 60min, inactivating enzyme, and cooling to room temperature; adding neutral protease 0.5 wt% of the powder, hydrolyzing at 40 deg.C for 100min, inactivating enzyme, and cooling to room temperature; adding alkaline protease 0.5 wt% of the powder, hydrolyzing at 40 deg.C for 100min, inactivating enzyme, and cooling to room temperature; adding activated carbon accounting for 0.5 percent of the weight of the powder for decoloring for half an hour, centrifugally filtering, collecting filtrate, filtering the filtrate by a 0.22 mu m microporous filter membrane to remove impurities, respectively filtering the filtrate by hollow fiber membranes with the cut-off amounts of 10kDa, 5kDa and 3kDa and an ultrafiltration membrane with the cut-off amount of 1kDa, filtering components with the molecular weight of below 1kDa by an ultrafiltration membrane with the molecular weight of 200Da for removing impurities, carrying out low-temperature rotary evaporation on the obtained polypeptide with each molecular weight section, carrying out cold drying, and selecting moisturizing polypeptide with the molecular weight section of Mr <1 kDa.
2. The skin-moistening shower gel comprises the following raw materials in parts by weight: 2-5% of decyl glucoside, 1-3% of cocamidopropyl betaine, 0.5-1.5% of sodium dodecyl sulfate, 1-5% of glycerol, 0.5-1.5% of behenyl alcohol, 0.5-1.5% of caprylyl glycol, 1-3% of sodium carboxymethyl cellulose, 3-5% of squalane, 1-2% of L-fucose, 0.25-1% of sodium hyaluronate, 1-2% of moisturizing polypeptide and the balance of deionized water; wherein the moisturizing polypeptide is prepared by adopting the following method: the method comprises the following steps of taking cactus powder and n-hexane according to the volume ratio of 1: mixing at a ratio of 3, extracting with shaking in 40 deg.C water bath for 30min, centrifuging at 10000g/min for 3min, discarding supernatant, adding n-hexane again, repeating for 1 time to completely remove fat, placing in a constant temperature drying oven, and drying at 65 deg.C to constant weight to obtain powder; adding 10 times of deionized water according to the volume ratio of the powder, adjusting pH to 8.5, adding 0.2% of cellulase and 0.3% of pectinase, hydrolyzing at 50 deg.C for 60min, inactivating enzyme, and cooling to room temperature; adding neutral protease 0.5 wt% of the powder, hydrolyzing at 40 deg.C for 100min, inactivating enzyme, and cooling to room temperature; adding alkaline protease 0.5 wt% of the powder, hydrolyzing at 40 deg.C for 100min, inactivating enzyme, and cooling to room temperature; adding activated carbon accounting for 0.5 percent of the weight of the powder for decoloring for half an hour, centrifugally filtering, collecting filtrate, filtering the filtrate by a 0.22 mu m microporous filter membrane to remove impurities, respectively filtering the filtrate by hollow fiber membranes with the cut-off amounts of 10kDa, 5kDa and 3kDa and an ultrafiltration membrane with the cut-off amount of 1kDa, filtering components with the molecular weight of below 1kDa by an ultrafiltration membrane with the molecular weight of 200Da for removing impurities, carrying out low-temperature rotary evaporation on the obtained polypeptide with each molecular weight section, carrying out cold drying, and selecting moisturizing polypeptide with the molecular weight section of Mr <1 kDa.
3. The body wash according to claim 2, wherein other moisturizing factors can be added to increase the moisturizing effect.
4. Use of a moisturizing polypeptide in the preparation of a cosmetic or a medicament for repairing skin damage; the moisturizing polypeptide is prepared by the following method: the method comprises the following steps of taking cactus powder and n-hexane according to the volume ratio of 1: mixing at a ratio of 3, extracting with shaking in 40 deg.C water bath for 30min, centrifuging at 10000g/min for 3min, discarding supernatant, adding n-hexane again, repeating for 1 time to completely remove fat, placing in a constant temperature drying oven, and drying at 65 deg.C to constant weight to obtain powder; adding 10 times of deionized water according to the volume ratio of the powder, adjusting pH to 8.5, adding 0.2% of cellulase and 0.3% of pectinase, hydrolyzing at 50 deg.C for 60min, inactivating enzyme, and cooling to room temperature; adding neutral protease 0.5 wt% of the powder, hydrolyzing at 40 deg.C for 100min, inactivating enzyme, and cooling to room temperature; adding alkaline protease 0.5 wt% of the powder, hydrolyzing at 40 deg.C for 100min, inactivating enzyme, and cooling to room temperature; adding activated carbon accounting for 0.5 percent of the weight of the powder for decoloring for half an hour, centrifugally filtering, collecting filtrate, filtering the filtrate by a 0.22 mu m microporous filter membrane to remove impurities, respectively filtering the filtrate by hollow fiber membranes with the cut-off amounts of 10kDa, 5kDa and 3kDa and an ultrafiltration membrane with the cut-off amount of 1kDa, filtering components with the molecular weight of below 1kDa by an ultrafiltration membrane with the molecular weight of 200Da for removing impurities, carrying out low-temperature rotary evaporation on the obtained polypeptide with each molecular weight section, carrying out cold drying, and selecting moisturizing polypeptide with the molecular weight section of Mr <1 kDa.
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| CN111067816A (en) * | 2020-01-15 | 2020-04-28 | 上海中匡生物科技有限公司 | Moisturizing and repairing mask containing active polypeptide and preparation method thereof |
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| CN1237982C (en) * | 2003-03-26 | 2006-01-25 | 王志忠 | Cactus primary juice and its preparation method and application |
| CN103417414A (en) * | 2012-05-20 | 2013-12-04 | 李一凡 | Extracting method for cactus primary juice |
| US20160243023A1 (en) * | 2015-02-19 | 2016-08-25 | Elc Management Llc | Novel Skin Remodeling Strategy |
| CN106511136B (en) * | 2016-12-23 | 2019-11-19 | 广州市科能化妆品科研有限公司 | Moisturizing cosmetic additive and preparation method thereof and cosmetics comprising the moisturizing cosmetic additive |
| CN107362090B (en) * | 2017-08-17 | 2020-02-21 | 广州市科能化妆品科研有限公司 | Water-based cosmetic capable of generating pearly luster and preserving moisture for long time |
| CN111067852A (en) * | 2020-01-16 | 2020-04-28 | 猫头鹰(福州)日用品有限公司 | Essence containing polypeptide and preparation method thereof |
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| CN111067816A (en) * | 2020-01-15 | 2020-04-28 | 上海中匡生物科技有限公司 | Moisturizing and repairing mask containing active polypeptide and preparation method thereof |
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