CN109481513A - A kind of Chinese medicine peptide and preparation method thereof for preventing and treating pig blue-ear disease - Google Patents
A kind of Chinese medicine peptide and preparation method thereof for preventing and treating pig blue-ear disease Download PDFInfo
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Abstract
The present invention relates to veterinary pharmaceutical processing technique fields, a kind of Chinese medicine peptide and preparation method for preventing and treating pig blue-ear disease is specifically disclosed, which is made after cellulase, amylase and protease hydrolyzed by radix bupleuri, schizonepeta, Chinese ephedra, Radix Angelicae Sinensis, Radix Paeoniae Alba, Rhizoma Atractylodis Macrocephalae and radix saposhnikoviae.Chinese medicine peptide dosage of the present invention is few, short treating period, has significant prevention and treatment effect to pig blue-ear disease, and be not likely to produce drug resistance, no drug residue in pork.
Description
Technical field
The present invention relates to veterinary pharmaceutical processing technique fields, and in particular to a kind of Chinese medicine peptide and its system for preventing and treating pig blue-ear disease
Preparation Method.
Background technique
Pig blue-ear disease is also known as porcine reproductive and respiratory syndrome, is the main infection that current serious restricts that pig breeding industry develops in a healthy way
Disease, major transmission path are that contact infection, air borne and sperm are propagated, can also be by placenta vertical transmission, from the nose of sick pig
Virus can be detected in chamber, excrement and urine.Various ages in days, kind pig can infect this virus, wherein age in days January with
Interior piglet and pregnant sow is most susceptible swinery.Morbidity sow is mainly shown as spiritual depressed, poor appetite or goes on a hunger strike, sends out
There is different degrees of expiratory dyspnea in heat, and pregnant sow is easily miscarried, and for abortion ratio up to 50%~70%, mortinatality is reachable
35% or more, mummy is up to 25%, and the death rate obviously increases (40%~80%) in 1 week part newborn piglet postpartum.Growth
Pig and growing and fattening pigs show different degrees of Respiratory symptoms, and a small number of cases can express cough and the ears back side, edge, abdomen
There is darkviolet in portion and tail skin.Secondary infection easily occurs for infection pig, and corresponding symptom occurs.Pig infects this after being ill, then
There is onset anxious, clinical symptoms are serious, easily the higher feature of Spreading and diffusion and case fatality rate and the death rate, endanger to aquaculture
Evil is very big.
Mainly prevented at present by vaccine for pig blue-ear disease, lacks the drug that can effectively prevent the disease.
Summary of the invention
For the above state of the art, the present invention, which provides one kind, can effectively prevent the Chinese medicine peptide of pig blue-ear disease, and provide it
Preparation method.
To achieve the above object of the invention, present invention employs the following technical solutions:
A kind of Chinese medicine peptide for preventing and treating pig blue-ear disease is to be made by the Chinese medicine material of following parts by weight through digesting: radix bupleuri
4-12 parts, 4-12 parts of schizonepeta, 4-12 parts of Chinese ephedra, 2-6 parts of Radix Angelicae Sinensis, 2-6 parts of Radix Paeoniae Alba, 2-6 parts of Rhizoma Atractylodis Macrocephalae, 2-6 parts of radix saposhnikoviae；The enzyme
For cellulase, amylase and protease.
In Chinese medicine material selected by the present invention, radix bupleuri evacuation bring down a fever, only malaria, soothing liver-qi stagnation, schizonepeta wind-dispelling, it is antipyretic,
Cool blood, Chinese ephedra diuresis, antibechic, heart tonifying, Radix Angelicae Sinensis relax bowel and defecation, activating microcirculation and removing stasis medicinal, anti-inflammatory, Radix Paeoniae Alba calming the liver and relieving pain, blood tonification effect, Rhizoma Atractylodis Macrocephalae
Strengthening the spleen and replenishing qi, eliminate dampness and have diuretic effect, windproof inducing diaphoresis to dispel wind win wet, relieving convulsion, and all medicines share keeps pharmacological property complementary, maximizes favourable factors and minimizes unfavourable ones, play disappear altogether
The effect of inflammation sterilization, activating microcirculation and removing stasis medicinal, has significant effect to prevention and treatment pig blue-ear disease.The present invention only selects seven kinds of Chinese medicine materials,
It avoids flavour of a drug excessive and leads to the problem of pharmacological property restriction or checking relation in five elements and prepare difficulty, and there is excellent control efficiency to pig blue-ear disease.
Selected Chinese medicine can be such that the effective component in medicinal material sufficiently discharges after digesting, such as saikoside, pulegone, fiber crops
The active Chinese drug components substances such as yellow alkali, ligusticum sinense oliver lactones, nopinene, australene, albiflorin, atractylol, atractylone, mannitol and bitter taste glycosides
And Multi-vitamin and multi-mineral substance etc., it can be utmostly from extracting into the cell, so that drug effect be made to significantly increase.Egg contained in medicinal material
White matter generates the lesser peptide of molecular weight after digesting, and can form chela with the active material and Multi-vitamin and multi-mineral progress chelating in Chinese medicine
The active peptide of conjunction state can reduce the mutual antagonism between active material, animal is enable quickly to absorb, and improve biological utilisation
Degree, achievees the purpose that quickly to treat.Three enzyme combined degradations, make the polysaccharide conversion oligosaccharide in Chinese medicine, and animal is easier to absorb
It utilizes.Chinese medicine can also generate some new active materials after digesting, and degrade toxic component and anaphylactogen, pacify product more
Entirely, absorptivity is higher, so that the Chinese medicine for digesting control efficiency more not significantly improves.
Preferably, the protease is neutral proteinase, and further preferably aspergillus oryzae produces neutral proteinase.
Preferably, the cellulase is Li Shi trichoderma cellulase-producing, and the amylase is Aspergillus Niger amylase.
Preferably, the dosage of the cellulase is the 0.8~1.2% of the Chinese medicine material gross mass, the amylase
Dosage be the 1~1.5% of the Chinese medicine material gross mass, the dosage of the protease is the 1 of the Chinese medicine material gross mass
~1.5%.
Preferred protease has preferable degradation effect to the protein of selected Chinese medicine, and it is higher small can to obtain activity
Molecular peptide, and can be digested in neutral conditions, avoid influence of the acid-base property condition to active material.Preferred each enzyme
Dosage can farthest reduce influencing each other between enzyme, guarantee the abundant progress of enzyme digestion reaction.
The preparation method of the Chinese medicine peptide of the prevention and treatment pig blue-ear disease, at least includes the following steps:
(1) each Chinese medicine material is weighed by the parts by weight, adds water to cook to obtain decoction liquor；
(2) decoction liquor is cooled to 56~58 DEG C, is separately added into the cellulase, amylase and protease, stirs
Enzymatic hydrolysis is mixed, then heat up enzyme deactivation, obtains enzymolysis liquid；
(3) enzymolysis liquid is filtered, the Chinese medicine peptide of the prevention and treatment pig blue-ear disease is obtained after the purified drying of filtrate.
Preparation method of the invention is simple, mild condition, is conducive to the production of heavy industrialization.
Preferably, in step (1), amount of water is 7~8 times of the Chinese medicine material gross mass, decocts 7 at 90~95 DEG C
~8h.
Preferably, in step (2), the enzymolysis time is 3~4h, and the heating enzyme deactivation is to keep 10 at 90~95 DEG C
~15min.
Preferably, in step (3), the filtering uses decanter centrifuge, and the purifying is by filtrate three-phase centrifuge
Centrifugation, then be concentrated through dual-effect concentrator, concentrate is finally spray-dried to obtain the Chinese medicine peptide for preventing and treating pig blue-ear disease.
Decanter centrifuge is suitble to the filtering of thick liquid, more efficient than flame filter press, preliminary by decanter centrifuge
It is filtered to remove insoluble matter, grease and other suspended matters in enzymolysis liquid are then removed by three-phase centrifuge, separation can be improved
Efficiency improves the purity of product Chinese medicine peptide.
The drug that various dosage forms are made in conventional pharmaceutical auxiliary material can also be added in Chinese medicine peptide of the present invention, as powder, oral solution,
Granula, tablet, pill or paste etc..
Chinese medicine peptide usage amount of the present invention is few, and the 1/50~1/30 of only conventional Chinese medicine usage amount, and short treating period, it can be complete
Instead of antibiotic, treatment cost is lower, and will not make pathogenic bacteria generation drug resistance, residual without any medicine in pork.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment 1
Radix bupleuri 400g, schizonepeta 800g, Chinese ephedra 400g, Radix Angelicae Sinensis 600g, Radix Paeoniae Alba 200g, Rhizoma Atractylodis Macrocephalae 400g, radix saposhnikoviae 200 are chosen respectively
Gram, 21L purified water is added and stirs evenly, under the conditions of 90 DEG C, decocts 7h；Then 56 DEG C are cooled to, 30g aspergillus niger is separately added into
Amylase, 30g aspergillus oryzae neutral proteinase and 24g Li Shi trichoderma cellulase-producing, stirring enzymatic hydrolysis 3.0h, then 90 DEG C are warming up to,
Keep 10min enzyme deactivation；First enzymolysis liquid is filtered with sleeping gong centrifuge after enzyme deactivation；Three-phase centrifuge Purification by filtration is used again；Filtrate is used
Dual-effect concentrator is further concentrated into the 1/3 of original volume；The Chinese medicine of prevention and treatment pig blue-ear disease is arrived after concentrate is spray-dried
Peptide product.
Embodiment 2
Radix bupleuri 8kg, schizonepeta 4kg, Chinese ephedra 12kg, Radix Angelicae Sinensis 2kg, Radix Paeoniae Alba 4kg, Rhizoma Atractylodis Macrocephalae 6kg, radix saposhnikoviae 4kg are chosen respectively, are added
300L purified water stirs evenly, under the conditions of 92 DEG C, boiling 7.5h；Then 57 DEG C are cooled to, 500g aspergillus niger shallow lake is separately added into
Powder enzyme, 600g aspergillus oryzae neutral proteinase and 400g cellulase, stirring enzymatic hydrolysis 3.5h, then 92 DEG C are warming up to, keep 12min to go out
Enzyme；First with sleeping gong centrifuge filtering after enzyme deactivation；Three-phase centrifuge Purification by filtration is used again；Filtrate is further dense with dual-effect concentrator
Contracting；The Chinese medicine peptide product for the treatment of pig blue-ear disease is arrived after concentrate spray-dried separately.
Embodiment 3
Radix bupleuri 120kg, schizonepeta 120kg, Chinese ephedra 80kg, Radix Angelicae Sinensis 40kg, Radix Paeoniae Alba 60kg, Rhizoma Atractylodis Macrocephalae 20kg, radix saposhnikoviae are chosen respectively
60kg is added 4000L purified water and stirs evenly, under the conditions of 95 DEG C, boiling 8.0h；Then 58 DEG C are cooled to, is separately added into
7.5kg aspergillus niger amylase, 7.5kg aspergillus oryzae neutral proteinase and 6kg cellulase, stirring enzymatic hydrolysis 4.0h, then it is warming up to 95
DEG C, keep 15min enzyme deactivation；First with sleeping gong centrifuge filtering after enzyme deactivation；Three-phase centrifuge Purification by filtration is used again；Filtrate is dense with economic benefits and social benefits
Contracting device is further concentrated；The Chinese medicine peptide product for the treatment of pig blue-ear disease is arrived after concentrate spray-dried separately.
Comparative example 1
Rhizoma Atractylodis Macrocephalae in embodiment 1 is substituted for the radix scutellariae of equivalent, remaining and embodiment 1 are identical, and Chinese medicine peptide is made.
Comparative example 2
Radix Angelicae Sinensis in embodiment 1 is substituted for the radix rehmanniae preparata of equivalent, remaining and embodiment 1 are identical, and Chinese medicine peptide is made.
In order to embody the effect of Chinese medicine peptide of the present invention, the prevention and treatment for being respectively used to pig blue-ear disease are tested, above
Chinese medicine peptide made from embodiment and comparative example is for when preventing pig blue-ear disease, every consumption per day to be 1mg/kg weight, for treating pig
When blue otopathy, every consumption per day is 5mg/kg weight.
Test example 1
Selecting the weight of health is grower pigs 300 of 30kg or so, is divided into six groups, every group 50, administration is implemented respectively
Each Chinese medicine peptide is dissolved in drinking water by the Chinese medicine peptide of example 1~3 and comparative example 1~2, and pig passes through drinking-water medication, blank control group
Normal water.After 7d is administered, PRRS virus liquid is sprayed in pig house, statistics infection rate (following pig blue-ear disease occurs after 3d
Symptom is appraisal basis: apathetic, fever, difficulty of being short of breath), the results are shown in Table 1.
The effect of 1 Chinese medicine peptide of table prevention pig blue-ear disease
| Group | The total head number of pig | Infect head number | Infection rate/% |
| Blank control | 50 | 42 | 84 |
| Embodiment 1 | 50 | 6 | 12 |
| Embodiment 2 | 50 | 5 | 10 |
| Embodiment 3 | 50 | 8 | 16 |
| Comparative example 1 | 50 | 17 | 34 |
| Comparative example 2 | 50 | 21 | 42 |
By 1 result of table as it can be seen that the Chinese medicine peptide of the present invention and comparative example can significantly reduce infection rate compared with blank control group, with
Comparative example is compared, and the effect that Chinese medicine peptide made from the embodiment of the present invention reduces infection rate is more excellent.
Test example 2
The piglet on certain pig farm in August, 2017 occur it is apathetic, be short of breath, abdominal respiration, dermohemia is in indigo plant
The symptoms such as purple, choose sick pig 210, be divided into seven groups, every group 30, blank control group is not administered, positive controls according to
20mg/kg weight gives Florfenicol, remaining gives the Chinese medicine peptide of Examples 1 to 3 and comparative example 1~2 respectively, unites after 5d is administered
Experimental result is counted, the results are shown in Table 2.
The effect of 2 Chinese medicine peptide of table treatment pig blue-ear disease
| Group | Sick pig head number | Cure head number | Dead head number | Effective percentage/% |
| Blank control | 30 | 0 | 15 | 13.3 |
| Positive control | 30 | 22 | 1 | 93.3 |
| Embodiment 1 | 30 | 29 | 0 | 100 |
| Embodiment 2 | 30 | 27 | 0 | 100 |
| Embodiment 3 | 30 | 28 | 0 | 100 |
| Comparative example 1 | 30 | 20 | 4 | 80 |
| Comparative example 2 | 30 | 22 | 3 | 83.3 |
By the result of table 2 as it can be seen that compared with blank control group, administration group has a significant curative effect, the Chinese medicine peptide of comparative example compared with
The effects of antibiotics of positive controls is slightly worse, and the Chinese medicine peptide of Examples 1 to 3 has then reached effect more preferably than antibiotic, and
Pig is without any side effects during medication and adverse reaction, is not likely to produce drug resistance after medication.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modification, equivalent replacement or improvement etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (10)
1. a kind of Chinese medicine peptide for preventing and treating pig blue-ear disease, which is characterized in that be made by the Chinese medicine material of following parts by weight through digesting:
4-12 parts of radix bupleuri, 4-12 parts of schizonepeta, 4-12 parts of Chinese ephedra, 2-6 parts of Radix Angelicae Sinensis, 2-6 parts of Radix Paeoniae Alba, 2-6 parts of Rhizoma Atractylodis Macrocephalae, 2-6 parts of radix saposhnikoviae；It is described
Enzyme be cellulase, amylase and protease.
2. the Chinese medicine peptide of prevention and treatment pig blue-ear disease as described in claim 1, it is characterised in that: the protease is neutral protein
Enzyme.
3. the Chinese medicine peptide of prevention and treatment pig blue-ear disease as claimed in claim 2, it is characterised in that: the amylase is Aspergillus Niger shallow lake
Powder enzyme, the neutral proteinase are that aspergillus oryzae produces neutral proteinase, and the cellulase is Li Shi trichoderma cellulase-producing.
4. the Chinese medicine peptide of prevention and treatment pig blue-ear disease as described in claim 1, it is characterised in that: the dosage of the cellulase is institute
The 0.8~1.2% of Chinese medicine material gross mass is stated, the dosage of the amylase is the 1~1.5% of the Chinese medicine material gross mass,
The dosage of the protease is the 1~1.5% of the Chinese medicine material gross mass.
5. the preparation method of the Chinese medicine peptide of the described in any item prevention and treatment pig blue-ear diseases of Claims 1 to 4, which is characterized in that at least
The following steps are included:
(1) each Chinese medicine material is weighed by the parts by weight, adds water to cook to obtain decoction liquor；
(2) decoction liquor is cooled to 56~58 DEG C, is separately added into the cellulase, amylase and protease, stir enzyme
Solution, then heat up enzyme deactivation, obtains enzymolysis liquid；
(3) enzymolysis liquid is filtered, the Chinese medicine peptide of the prevention and treatment pig blue-ear disease is obtained after the purified drying of filtrate.
6. the preparation method of the Chinese medicine peptide of prevention and treatment pig blue-ear disease as claimed in claim 5, it is characterised in that: in step (1), add
Water is 7~8 times of the Chinese medicine material gross mass, and 7~8h is decocted at 90~95 DEG C.
7. the preparation method of the Chinese medicine peptide of prevention and treatment pig blue-ear disease as claimed in claim 5, it is characterised in that: in step (2), institute
Stating enzymolysis time is 3~4h, and the heating enzyme deactivation is 10~15min of holding at 90~95 DEG C.
8. the preparation method of the Chinese medicine peptide of prevention and treatment pig blue-ear disease as claimed in claim 5, it is characterised in that: in step (3), institute
Filtering is stated using decanter centrifuge, the purifying is to be centrifuged filtrate with three-phase centrifuge, then be concentrated through dual-effect concentrator, finally
Concentrate is spray-dried to obtain the Chinese medicine peptide for preventing and treating pig blue-ear disease.
9. a kind of drug for preventing and treating pig blue-ear disease, which is characterized in that blue including the described in any item prevention and treatment pigs of Claims 1 to 4
The Chinese medicine peptide of otopathy.
10. as claimed in claim 9 prevention and treatment pig blue-ear disease drug, which is characterized in that its dosage form be powder, oral solution,
Granula, tablet, pill or paste.
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Cited By (2)
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110338265A (en) * | 2019-08-22 | 2019-10-18 | 河北肽都生物科技有限公司 | A kind of additive for feed for piglets and preparation method thereof |
| CN110338266A (en) * | 2019-08-26 | 2019-10-18 | 河北肽都生物科技有限公司 | One broad sow Chinese medicine complex peptides feed addictive and preparation method thereof |
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