CN109431847A - A kind of peptide composition and its application - Google Patents
A kind of peptide composition and its application Download PDFInfo
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- CN109431847A CN109431847A CN201811526833.6A CN201811526833A CN109431847A CN 109431847 A CN109431847 A CN 109431847A CN 201811526833 A CN201811526833 A CN 201811526833A CN 109431847 A CN109431847 A CN 109431847A
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- peptide composition
- facial mask
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/524—Preservatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Abstract
The invention belongs to cosmetic technical fields, and in particular to a kind of peptide composition and its application.The peptide composition includes palmityl tripeptides -1, -1 copper of palmityl tripeptides -5, epithelical cell growth factor and tripeptides.The peptide composition can be used for preparing cosmetics, and the present invention also provides one kind to repair facial mask containing peptide composition, mainly consist of the following compositions: peptide composition, the anti-irritant factor, pH adjusting agent, absorption enhancers, moisturizer, thickener, preservative and water.The absorption enhancers that facial mask of the invention uses can increase the stability of peptide composition, this contain peptide composition repair facial mask have thicken epidermis, skin is stimulated to generate collagen, elastin laminin and at laminins, accelerate the metabolism of Skin Cell, the reparation and regeneration for promoting impaired epidermis, the effect that face wrinkles can be eliminated, acne print, promote wound healing, desalinate scar.
Description
Technical field
The invention belongs to cosmetic technical fields, and in particular to a kind of peptide composition and its application.
Background technique
In recent years, the kind of cosmetics is increasing, functional also increasingly stronger.Facial mask is one in daily cosmetics
Kind, by its fitting contact action of short duration to face, it is temporarily isolating extraneous air and pollution.Isolation of short duration in this way can be with
Skin temperature is improved, the pore of skin is expanded, and then promotes the secretion and metabolism of sweat gland, is conducive to skin and excludes epidermis
The noxious products of cell metabolism and the oil substances of accumulation.Increase the oxygen content of skin simultaneously, in facial mask
Moisture penetrate into the cuticula of epidermis, so that skin is become soft and naturally bright and high resilience.But it is wide with cosmetics
The abuse of the spatter properties cosmetics such as general use, especially exfoliating, pore cleanser, keeps the cuticula of skin more and more thinner, leads to face
The exposure of portion's capillary, its moistening effect is worse, is easier to dry crack occur after cuticula is thinning, it is therefore desirable to which use can
Promote the skin-protection product of epidermal cell fast-growth, protects skin.
Patent document CN201710262266.7 discloses one kind and attains product collagen protein face, contains ultimate attainment reparation peptide, flesh
Peptide, biological carbohydrate gum, skin milk conditioner, dipotassium glycyrrhizinate, hydrolytic collagen, allantoin, preservative, citric acid, methyl hydroxybenzoate,
The substances such as Sodium Hyaluronate, EDETATE SODIUM, Cremophor RH40, essence have permeability, trophism, prosthetic, moisturizing
The effect of property, compatibility and whitening smoothing wrinkle, skin elasticity, pore refining can be enhanced, dispel facial wrinkles, sweep obscure oil
Light, the infiltration skin of energy deep layer, persistently supplements skin nutrition and moisture, repairs aging and damaged cell, reaches permanent beauty treatment effect
Fruit.In the collagen protein face in addition to containing peptide is repaired, also contain Sodium Hyaluronate, which is to maintain skin and the elasticity of muscle
Main matter, content directly determines the quality of skin, with strong moisture-keeping function, can maintain the tender light of keratoderma water
Sliding and high resilience, but hyaluronic acid is macromolecular viscous polysaccharide, it is difficult to skin corium is reached through cuticula and is worked, therefore real
Repairing effect is bad on border.In addition, containing a large amount of protein substances in the collagen protein face, influenced vulnerable to pH, temperature etc.
And deactivation, stability are poor, Yi Zisheng bacterium, to improve stability, are added to many stabilizers in the collagen protein face
Such as biological carbohydrate gum -1, dipotassium glycyrrhizinate, citric acid, EDETATE SODIUM, Cremophor RH40, methyl hydroxybenzoate and liquid preservative
Agent, complicated component, easily acceleration allergy, the thin crowd of especially not applicable cuticula are used for a long time.
Patent document CN201110127080.3 discloses a kind of transparent facial mask containing epidermal growth factor and its preparation
Method contains fibroblast growth factor, metallothionein, vitamin, skin conditioner, moisturizer, tackifier, pH in the facial mask
It is worth regulator and deionized water, the shield which has delay skin aging, repairs skin injury
Skin cosmetic composition, but epithelical cell growth factor is that polypeptide is similar with hyaluronic acid, penetrates the ability of keratoderma
It is weak, it is difficult to play a role into skin corium, therefore in this kind of cosmetology composition active principle utilization rate it is low, it is actually beautiful
Hold ineffective.
Therefore, still lack at present can stimulating cellular growth, repair damaged cell, thicken cuticula, anti-inflammatory antiallergy, together
When can pass through cuticula reach skin corium effect facial mask.
Summary of the invention
The present invention is intended to provide a kind of anti-inflammatory, antiallergy, can promote epidermal cell repairing and regeneration, thickens cuticula,
The easy peptide composition to work through epidermis arrival skin corium and its application, the peptide composition can be used for conduct of making up
Industry prepares face cream, facial mask or essence cream etc..For this purpose, the invention adopts the following technical scheme:
The present invention provides a kind of peptide compositions, including palmityl tripeptides -1, palmityl tripeptides -5, epidermal growth
- 1 copper of the factor and tripeptides.
Specifically, the palmityl tripeptides -1, the quality of -1 copper of palmityl tripeptides -5, epithelical cell growth factor and tripeptides
Than for (1-3): (2-5): (0.5-1.5): (1-2).
Further, the present invention also provides the peptide compositions to prepare the application in cosmetics.
Specifically, the present invention also provides one kind repairs facial mask containing peptide composition, mainly by following component and its quality
Percentage composition:
Peptide composition 3-6%, anti-irritant factor 1.5-2.5%, pH adjusting agent 0.05-0.12%, absorption enhancers 1-
2%, moisturizer 0.01-0.1%, thickener 0.1-0.2%, preservative 0.2-0.6% and water 88-95%.
Preferably, the reparation facial mask containing peptide composition is mainly made of following component and its mass percent:
Peptide composition 5%, the anti-irritant factor 2%, pH adjusting agent 0.1%, absorption enhancers 1.5%, moisturizer 0.05%,
Thickener 0.12%, preservative 0.4% and water 90.83%；The palmityl three that the peptide composition is 2:5:1:2 by mass ratio
Peptide -1, palmityl tripeptides -5, epithelical cell growth factor and -1 copper of tripeptides composition.
Preferably, the anti-irritant factor is the rough gentian anti-irritant factor.
Preferably, the absorption enhancers are rhamnolipid.
Preferably, the pH adjusting agent is triethanolamine, and the moisturizer is hyaluronic acid, and the thickener is card wave
Nurse 941, the preservative are parahydroxyacet-ophenone.
In addition, the present invention also provides the preparation methods that facial mask is repaired containing peptide composition, the preparation method is as follows:
S1: absorption enhancers, moisturizer, thickener and preservative are added to the water, and are impregnated 1-3h, are heated with stirring to 70-85
DEG C, revolving speed 30-50rpm stirs 6-10min, keeps the temperature 20-40min, obtains mixture A；
S2: it is cooled to 45-55 DEG C to mixture A in step S1, pH adjusting agent is added in 45-55 DEG C of water bath with thermostatic control, and revolving speed is
30-50rpm stirs 8-12min, obtains mixture B；
S3: being cooled to 34-36 DEG C to mixture B in step S2, and peptide composition is added in 34-36 DEG C of water bath with thermostatic control, is turning
Speed be 30-50rpm under the conditions of, stir 12-20min to get.
Peptide composition provided by the invention includes palmityl tripeptides -1, palmityl tripeptides -5, epithelical cell growth factor
With -1 copper of tripeptides, in specific implementation, the inventors discovered that, by palmityl tripeptides -1, palmityl tripeptides -5, epidermal growth because
The mass ratio of son and -1 copper of tripeptides is (1-3): (2-5): (0.5-1.5): (1-2) composition, the peptide composition have strong repair
The effect of damaged cell can reach the effect for promoting wound healing, which be used for cosmetic industry, can be made into face
The cosmetic product such as film, face cream or essence cream quickly repair damaged cell, desalination wrinkle even scar.
Rhamnolipid provided in the present invention containing in peptide composition reparation facial mask is by pseudomonad or Burkholderia
A kind of biosurfactant for biological metabolism property that class generates, stability is good, nontoxic, biodegradable, is a kind of good
Good wetting agent, the prior art does not report that it can promote Transdermal absorption effect, in reparation containing peptide composition provided by the invention
It is found in facial mask, rhamnolipid is added, can promote hyaluronic acid and peptide composition through epidermis and reach skin corium, more effectively
Ground plays the effect that peptide composition promotes wound healing, may have hydrophilic radical and hydrophobic grouping with rhamnolipid, and saturating
Bright matter acid, epidermal growth factor, -1 copper of tripeptides and reparation peptide: palmityl tripeptides -1 and palmityl tripeptides -5 are with Hydrogenbond, mouse
Lee's glycolipid peptides molecular surface adhere to, promote through cuticula reach skin corium effect it is related.Meanwhile the addition of rhamnolipid
The stability for also adding epidermal growth factor, -1 copper of tripeptides, palmityl tripeptides -1 and palmityl tripeptides -5, makes peptide matters not
Vulnerable to pH value, temperature change and deactivation, reduce the addition of preservative.
The rough gentian anti-irritant factor of the invention by Guangdong Di Mei new material Science and Technology Ltd. provide, it is interior containing propylene glycol,
Radix Gentianae extract and water, Radix Gentianae extract master constituents containing iridoid glycoside, and gentiamarin is effective component in iridoid.
The rough gentian anti-irritant factor is added in facial mask by the present invention has anti-inflammatory, analgesia, anti-irritant, antipruritic and antianaphylactic effect, from
And solves the problems, such as the inadaptability of easy allergy skin quality crowd.In addition, the present inventors have additionally discovered that, which can
The hygroscopicity of carbomer is reduced, effectively inhibits the growth of bacterium, reduces the additional amount of preservative, is solved after carbomer moisture absorption easily
In agglomeration, causes component distributing uneven, be not easy the case where absorbing.
In addition, it is found through experiment that, active peptide of the invention, which repairs facial mask, also has the function of anti-acne, desalination acne print, is applicable in
The patient of Yu Yichang small pox.
Compared with prior art, the invention has the following advantages that
(1) activity provided by the invention repairs peptide facial mask, and there is stimulation skin to generate collagen, elastin laminin and at glutinous
The effects of even albumen thickens epidermis can accelerate the metabolism of Skin Cell, promote the reparation and regeneration of impaired epidermis, except tool
There are desalination face wrinkles, desalinate outside scar, may additionally facilitate wound healing, eliminates acne print.
(2) activity produced by the present invention repairs the rhamnolipid that peptide facial mask uses, with promotion epidermal growth factor and thoroughly
Effective component epidermal growth factor, -1 copper of tripeptides, the stability for repairing peptide also can be improved in the Transdermal absorption effect of bright matter acid.
(3) active peptide facial mask produced by the present invention contains the rough gentian anti-irritant factor, has anti-inflammatory and antalgic, anti-irritant and anti-mistake
Quick effect, there is fungistatic effect, and the stability of carbomer can be improved, and is uniformly distributed facial mask component, extends the facial mask shelf-life.
Specific embodiment
The specific embodiment of form by the following examples makees further specifically above content of the invention
It is bright.But the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to following embodiment.
Embodiment 1, a kind of peptide composition that contains repair facial mask
It is described to repair facial mask containing peptide composition, it is made of following component and its mass percent:
Peptide composition 3%, the rough gentian anti-irritant factor 1.5%, triethanolamine 0.05%, rhamnolipid 1%, hyaluronic acid
0.01%, Carbopol 941 0.1%, parahydroxyacet-ophenone 0.2% and water 94.14%.
The peptide composition is thin by the palmityl tripeptides -1, palmityl tripeptides -5, epidermis that mass ratio is 3:4:0.5:1
The intracellular growth factor and -1 copper of tripeptides composition.
The membrane preparation method of repair surface containing peptide composition is as follows:
S1: rhamnolipid, hyaluronic acid, Carbopol 941 and parahydroxyacet-ophenone are added to the water, and impregnate 4h, and stirring adds
Heat stirs 8min to 80 DEG C, revolving speed 45rpm, keeps the temperature 30min, obtains mixture A；
S2: being cooled to 50 DEG C to mixture A in step S1,50 DEG C of waters bath with thermostatic control, is added triethanolamine, revolving speed 30rpm,
10min is stirred, mixture B is obtained；
S3: being cooled to 35 DEG C to mixture in step S2,35 DEG C of waters bath with thermostatic control, be added repair peptide, epidermal growth because
Son, -1 copper of the anti-irritant factor and tripeptides stir 15min to get gel-type vehicle, then by gel base under the conditions of revolving speed is 30rpm
Matter is spread evenly across coating, covers back sheet, compacting, encapsulation to get.
Embodiment 2, a kind of peptide composition that contains repair facial mask
The reparation facial mask containing peptide composition is made of following component and its mass percent:
Peptide composition 6%, the rough gentian anti-irritant factor 2.5%, triethanolamine 0.12%, rhamnolipid 2%, hyaluronic acid
0.1%, Carbopol 941 0.2%, parahydroxyacet-ophenone 0.6% and water 88.48%.
Palmityl tripeptides -1, the palmityl tripeptides -5, epidermis that the peptide composition is 1:5:0.5:1.5 by mass ratio
Porcine HGF and -1 copper of tripeptides composition.
Preparation method is similar to Example 1.
Embodiment 3, a kind of peptide composition that contains repair facial mask
The reparation facial mask containing peptide composition is made of following component and its mass percent:
Peptide composition 5%, the rough gentian anti-irritant factor 2%, triethanolamine 0.1%, rhamnolipid 1.5%, hyaluronic acid
0.05%, Carbopol 941 0.12%, parahydroxyacet-ophenone 0.4% and water 90.93%.
The peptide composition is raw by the palmityl tripeptides -1, palmityl tripeptides -5, epidermal cell that mass ratio is 2:5:1:2
The long factor and -1 copper of tripeptides composition.
Preparation method is similar to Example 1.
Comparative example 1, a kind of peptide composition that contains repair facial mask
The reparation facial mask containing peptide composition is made of following component and its mass percent:
Peptide composition 5%, the rough gentian anti-irritant factor 2%, triethanolamine 0.1%, hyaluronic acid 0.05%, carbomer
9410.12%, parahydroxyacet-ophenone 0.4% and water 92.43%.
The peptide composition is raw by the palmityl tripeptides -1, palmityl tripeptides -5, epidermal cell that mass ratio is 2:5:1:2
The long factor and -1 copper of tripeptides composition.
Preparation method is similar to Example 1.
Difference with embodiment 3 is to be not added with rhamnolipid.
Comparative example 2, a kind of peptide composition that contains repair facial mask
The reparation facial mask containing peptide composition is made of following component and its mass percent:
Peptide composition 5%, the rough gentian anti-irritant factor 2%, triethanolamine 0.1%, menthol 1.5%, hyaluronic acid
0.05%, Carbopol 941 0.12%, parahydroxyacet-ophenone 0.4% and water 90.93%.
The peptide composition is raw by the palmityl tripeptides -1, palmityl tripeptides -5, epidermal cell that mass ratio is 2:5:1:2
The long factor and -1 copper of tripeptides composition.
Preparation method is similar to Example 1.
Difference with embodiment 3 is to replace rhamnolipid with menthol.
Comparative example 3, a kind of peptide composition that contains repair facial mask
The reparation facial mask containing peptide composition is made of following component and its mass percent:
Peptide composition 5%, strontium cation 2%, triethanolamine 0.1%, rhamnolipid 1.5%, hyaluronic acid 0.05%,
Carbopol 941 0.12%, parahydroxyacet-ophenone 0.4% and water 90.93%.
The peptide composition is raw by the palmityl tripeptides -1, palmityl tripeptides -5, epidermal cell that mass ratio is 2:5:1:2
The long factor and -1 copper of tripeptides composition.
Preparation method is similar to Example 1.
Difference with embodiment 3 is to replace the rough gentian anti-irritant factor with strontium cation.
Comparative example 4, a kind of peptide composition that contains repair facial mask
The reparation facial mask containing peptide composition is made of following component and its mass percent:
Peptide composition 5%, the rough gentian anti-irritant factor 2%, triethanolamine 0.1%, rhamnolipid 1.5%, hyaluronic acid
0.05%, Carbopol 941 0.12%, parahydroxyacet-ophenone 0.4% and water 90.93%.
Preparation method is similar to Example 1.
Difference with embodiment 3 is the peptide composition by the palmityl tripeptides -1 that mass ratio is 1:1:1:1, palm
Acyl tripeptides -5, epithelical cell growth factor and -1 copper of tripeptides composition.
Test example one promotes wound healing
1, test objective: the measurement embodiment 1-3 and comparative example 1-4 promotion wound obtained that facial mask is repaired containing peptide composition
Mouth healing effect.
2, test method:
SD male rat 80, every 220 ± 20g of weight are taken, random point 8 groups, every group 10, difference label, in back ridge
Column side, cuts off hair, is scratched with scalpel and grows 2 ± 0.2cm, and the edge of a knife of 0.2 ± 0.1cm of depth smears embodiment 1, reality respectively
It applies example 2 and embodiment 3 is obtained containing peptide composition reparation facial mask, comparative example 1, comparative example 2, comparative example 3 and comparative example 4 are made
Active peptide repair facial mask, blank control group does not use any drug, smears in the morning, afternoon and evening daily three times, on day 1, the 3rd day, the
7 days and the 15th day observation wound healing situations evaluate wound healing situation with Wound healing rate.
3, test result: Wound healing rate situation is shown in Table 1.
1 surface of a wound of table is cured rate (%)
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Blank control | |
| 1st day | 1.40 | 1.15 | 1.69 | 1.03 | 1.12 | 1.45 | 0.89 | 0.68 |
| 3rd day | 8.23 | 7.01 | 9.63 | 4.24 | 4.89 | 7.12 | 3.58 | 3.46 |
| 7th day | 58.20 | 56.43 | 61.23 | 40.54 | 41.23 | 55.21 | 28.32 | 30.26 |
| 10th day | 80.69** | 76.41** | 86.67** | 58.51* | 60.45* | 75.21** | 40.67 | 42.73 |
| 15th day | 90.60** | 93.36** | 98.48** | 78.59* | 80.21* | 89.92** | 70.38 | 68.75 |
Compared with blank control group: P < 0.01 * *；*P<0.05.
From result in table 1:
After (1) the 15th day, the embodiment 1-3 wound healing rate and blank control obtained that facial mask is repaired containing peptide composition
For group compared to there is significant difference (P < 0.01), healing rate all reaches 90% or more, has good promotion wound healing effect, separately
Outside, the reparation facial mask obtained containing peptide composition of comparative example 3 also shows good wound healing promoting effect, with control group phase
Than there is notable difference (P < 0.01), but in physical record, 3 groups of rats of comparative example have a little stimulation in the 1-3 days smearings
The phenomenon that wound, wound redness, but redness phenomenon disappears after 3 days.
(2) the from the 1st to the 15th day, comparative example 1-2 is obtained to repair facial mask compared with blank control group containing peptide composition
Variant (P < 0.05) has the effect of certain promotion wound healing, but wound healing rate is lower than embodiment；It is right
The indifference obtained compared with blank control group containing peptide composition reparation facial mask of ratio 4 is anisotropic, and the experimental result of each comparative example is said
It is bright to repair the substance that peptide is main promotion wound healing in the activity facial mask, especially when peptide composition palmityl tripeptides-
1, palmityl tripeptides -5, epithelical cell growth factor and -1 copper of tripeptides are (1-3) in mass ratio: (2-5): (0.5-1.5): (1-
2) combined effect is more preferable；The rough gentian anti-irritant factor has the function of antiallergy anti-irritant.
Test example two, irritation test
1, subjects: healthy guinea pig 60, weight 220-300g, male and female are half-and-half.
2, test material: embodiment 1-3 and the peptide composition that contains of the preparation of comparative example 3 repair facial mask.
3, test method:
Grouping: cavy is randomly divided into 6 groups, blank control group, example 1 group, 2 groups of embodiment, is implemented positive sensitization group
3 groups of example and 3 groups of comparative example, blank control group, every group 10, half-and-half, cavy body back is lost hair or feathers for male and female, and blank control group exists
0.5-0.6ml physiological saline is smeared at depilation；Positive sensitization group smears 0.5-0.6ml sensitization emulsifiable paste at depilation, uniformly smears
At cavy depilation；One kind prepared by embodiment 1-3 and comparative example 3 is repaired facial mask containing peptide composition and is smeared at depilation respectively
0.5-0.6ml observes phenomena of cutaneous irritation respectively at 0,1,6,24,48,72h.
4, test result:
Test result shows that blank control group, example 1 group, 2 groups of embodiment and 3 groups of embodiment each 10 cavys do not go out
Existing erythema and oedema；3 groups of 10 cavys of comparative example occur a small amount of small erythema in 1h, and when 6h subsides substantially；Sensitization group cavy is certainly
There is erythema after administration 1h, when 6h is most obvious, gradually subsides for 24 hours, erythema subsides substantially after 72h.As a result illustrate, rough gentian anti-irritant
Antiallergy, the anti-irritant effect of the factor are good, better than strontium cation commonly used in the prior art.
Test example three, anti-acne effect test
1, test material: embodiment 1-3, comparative example 1-3 are obtained to repair facial mask containing peptide composition.
2, subjects:
Number: 120 people；
Age: between 18-48 years old；
Gender: female；
Health status: the long acne of subjects skin has acne or has acne scar, no skin disease allergies, and subject's aspiration is selected,
Tested period, diet are pungent.
3, test method:
Subjects are randomly divided into 6 groups, every group of 20 people, respectively application embodiment 1, embodiment 2, embodiment 3, comparative example 1,
It is obtained in comparative example 2 and comparative example 3 to repair facial mask containing peptide composition, it applies 2-3 times weekly, after 1 month, observes tested
Small pox, acne, the situation of acne scar of personnel on the face.
Evaluation explanation:
(1) do not improve the small pox referred to subject on the face, acne, acne scar not eliminate, there is new small pox and acne to generate；
(2) slightly improve and refer to the phenomenon that small pox, the quantity of acne of tested crowd on the face are not further added by and are reduced, but acne
Scar is unchanged；
(3) it is obviously improved and refers to that small pox or acne have 1 or more to fully recover, acne scar has desalination；
(4) improve completely and refer to small pox, acne recovery from illness, the desalination of acne scar is obvious.
4, test result:
As a result such as table 2.
2 subject face situation of table record
| Group | Do not improve | Slightly improve | It is obviously improved | Improve completely |
| Embodiment 1 | 0 | 2 | 9 | 9 |
| Embodiment 2 | 0 | 2 | 7 | 11 |
| Embodiment 3 | 0 | 1 | 8 | 11 |
| Comparative example 1 | 2 | 8 | 8 | 2 |
| Comparative example 2 | 1 | 6 | 11 | 2 |
| Comparative example 3 | 0 | 10 | 8 | 2 |
Illustrated by table 2:
(1) growth of small pox can be effectively suppressed in the reparation facial mask containing peptide composition that 1-3 of the embodiment of the present invention is provided, and promotees
Into the healing of small pox, acne, desalinate acne print；
(2) repairing in facial mask in comparative example 1-3 containing peptide composition, anti-acne effect is best for comparative example 3, but phase
Facial mask is repaired than containing peptide composition made from embodiment 1-3 in the present invention, the ability for curing small pox is substantially reduced, and is illustrated more
The composition ratio of peptide combinations has an impact to skin repair.
(3) active peptide made from comparative example 1-2 repairs facial mask and embodiment 1-3 is obtained containing peptide composition reparation facial mask
Anti-acne effect compare, illustrate the addition of rhamnolipid in prescription can be obviously promoted small pox healing.
(4) in addition, the effect of comparative example 1 more preferably also illustrates that the promotion of rhamnolipid absorbs work than 2 healing effect of comparative example
With more preferable than menthol.
Test example four, stability test
1, subjects: what embodiment 3 and comparative example 2-3 were prepared repairs facial mask containing peptide composition.
2, test method: embodiment 3 and comparative example 3 be prepared containing peptide composition repair facial mask using aluminium foil bag+
Carton package is 25 ± 2 DEG C in temperature, and relative humidity is 60% ± 10%, carries out 6 months greenhouses and keeps sample test, to sample
Appearance, weight differential, microbial limit carry out investigation measurement.
3, test result:
Test result is as shown in Table 3 and Table 4.
3 appearance of table (25 ± 2 DEG C of temperature, relative humidity 60% ± 10%)
The stability test that facial mask is repaired containing peptide composition that 4 embodiment 3 of table is prepared
| 0 month | January | 2 months | March | June | |
| Total bacteria | Nothing | 10/5cm2 | 15/5cm2 | 15/5cm2 | 20/5cm2 |
| Mould, yeast count | Nothing | 1/5cm2 | 1/5cm2 | 2/5cm2 | 2/5cm2 |
| Staphylococcus aureus | It is not detected | It is not detected | It is not detected | It is not detected | It is not detected |
| Pseudomonas aeruginosa | It is not detected | It is not detected | It is not detected | It is not detected | It is not detected |
| Escherichia coli | It is not detected | It is not detected | It is not detected | It is not detected | It is not detected |
The membrane stability of repair surface containing the peptide composition test that 5 comparative example 2 of table is prepared
The membrane stability of repair surface containing the peptide composition test that 6 comparative example 3 of table is prepared
| 0 month | January | 2 months | March | June | |
| Total bacteria | Nothing | 10/5cm2 | 15/5cm2 | 31/5cm2 | 42/5cm2 |
| Mould, yeast count | Nothing | 1/5cm2 | 3/5cm2 | 5/5cm2 | 9/5cm2 |
| Staphylococcus aureus | It is not detected | It is not detected | It is not detected | It is not detected | Detection |
| Pseudomonas aeruginosa | It is not detected | It is not detected | It is not detected | It is not detected | Detection |
| Escherichia coli | It is not detected | It is not detected | It is not detected | It is not detected | Detection |
(1) by the appearance test result of table 3 it is found that at 25 ± 2 DEG C of temperature, 60% ± 10% environment of relative humidity lower 6
After month, what embodiment 3 was prepared repairs the brighter water profit of facial mask containing peptide composition, uniform color, has small agglomeration, but compares
The reparation facial mask containing peptide composition that example 2 and comparative example 3 are prepared is obscure, uneven color is even, has into fish-eye shaped agglomerates, and stablizes
Property it is poor, by 2 comparative descriptions rhamnolipid of comparative example by improve effective component peptide composition stability, the present invention can be increased
The stability that facial mask is repaired containing peptide composition, carbomer can be improved in the comparative descriptions rough gentian anti-irritant factor of comparative example 3
Stability is uniformly distributed facial mask component, extends the facial mask shelf-life.
(2) by the Comparative result of table 4-6 it is found that after 6 months, repair surface containing peptide composition that comparative example 3 is prepared
Film detects staphylococcus aureus, pseudomonas aeruginosa and Escherichia coli, and total bacteria, fungi count, yeast count are higher than real
It applies the peptide composition that contains that example 3 is prepared and repairs facial mask, do not meet facial mask long-term preservation quality standard, illustrate rough gentian anti-irritant
The factor has the effect of antibacterial, can extend the shelf-life of facial mask, and what comparative example 2 was prepared repairs facial mask not containing peptide composition
Detect staphylococcus aureus, pseudomonas aeruginosa and Escherichia coli, but total bacteria, fungi count, yeast count are higher than in fact
Example 3 is applied, comparative example 3 is lower than, illustrates rhamnolipid because epidermal growth factor, -1 copper of tripeptides, the ultimate attainment stabilization for repairing peptide can be increased
Property, make peptide matters be not easy to go mouldy.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe
The personage for knowing this technology all without departing from the spirit and scope of the present invention, carries out modifications and changes to above-described embodiment.Cause
This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as
At all equivalent modifications or change, should be covered by the claims of the present invention.
Claims (9)
1. a kind of peptide composition, which is characterized in that including palmityl tripeptides -1, palmityl tripeptides -5, epidermal growth because
Son and -1 copper of tripeptides.
2. peptide composition according to claim 1, which is characterized in that the palmityl tripeptides -1, palmityl tripeptides -5,
The mass ratio of -1 copper of epithelical cell growth factor and tripeptides is (1-3): (2-5): (0.5-1.5): (1-2).
3. peptide composition of any of claims 1 or 2 is preparing the application in cosmetics.
4. one kind repairs facial mask containing peptide composition, mainly it is made of following component and its mass percent:
As the peptide composition 3-6% of claims 1 or 2, anti-irritant factor 1.5-2.5%, pH adjusting agent 0.05-0.12%,
Absorption enhancers 1-2%, moisturizer 0.01-0.1%, thickener 0.1-0.2%, preservative 0.2-0.6% and water 88-95%.
5. according to claim 4 repair facial mask containing peptide composition, which is characterized in that mainly by following component and its matter
Measure percentage composition:
As the peptide composition 5% of claims 1 or 2, the anti-irritant factor 2%, pH adjusting agent 0.1%, absorption enhancers 1.5%,
Moisturizer 0.05%, thickener 0.12%, preservative 0.4% and water 90.83%, the peptide composition are 2:5 by mass ratio:
Palmityl tripeptides -1, palmityl tripeptides -5, epithelical cell growth factor and -1 copper of the tripeptides composition of 1:2.
6. according to claim 4 or 5 repair facial mask containing peptide composition, which is characterized in that the anti-irritant factor
For the rough gentian anti-irritant factor.
7. according to claim 4 or 5 repair facial mask containing peptide composition, which is characterized in that the absorption enhancers are
Rhamnolipid.
8. according to claim 4 or 5 repair facial mask containing peptide composition, which is characterized in that the pH adjusting agent is three
Ethanol amine, the moisturizer are hyaluronic acid, and the thickener is Carbopol 941, and the preservative is parahydroxyacet-ophenone.
9. -8 any preparation method for repairing facial mask containing peptide composition according to claim 1, which is characterized in that preparation
Method is as follows:
S1: absorption enhancers, hyaluronic acid, carbomer and parahydroxyacet-ophenone are added to the water, and are impregnated 1-3h, are heated with stirring to
70-85 DEG C, revolving speed 30-50rpm, 6-10min is stirred, 20-40min is kept the temperature, obtains mixture A；
S2: it is cooled to 45-55 DEG C to mixture A in step S1, triethanolamine, revolving speed 30- is added in 45-55 DEG C of water bath with thermostatic control
50rpm stirs 8-12min, obtains mixture B；
S3: it is cooled to 34-36 DEG C to mixture B in step S2,34-36 DEG C of water bath with thermostatic control is added and repairs peptide, epidermal growth
- 1 copper of the factor, the anti-irritant factor and tripeptides, revolving speed be 30-50rpm under the conditions of, stir 12-20min to get.
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| CN201811526833.6A CN109431847A (en) | 2018-12-13 | 2018-12-13 | A kind of peptide composition and its application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110200875A (en) * | 2019-07-09 | 2019-09-06 | 中山卡丝生物科技有限公司 | A kind of antiallergic whitening mask and preparation method thereof |
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2018
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110200875A (en) * | 2019-07-09 | 2019-09-06 | 中山卡丝生物科技有限公司 | A kind of antiallergic whitening mask and preparation method thereof |
| CN110200875B (en) * | 2019-07-09 | 2022-03-18 | 中山卡丝生物科技有限公司 | Anti-allergy whitening mask and preparation method thereof |
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