CN109430854B - Health product and preparation method thereof - Google Patents
Health product and preparation method thereof Download PDFInfo
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- CN109430854B CN109430854B CN201811088857.8A CN201811088857A CN109430854B CN 109430854 B CN109430854 B CN 109430854B CN 201811088857 A CN201811088857 A CN 201811088857A CN 109430854 B CN109430854 B CN 109430854B
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- dendrobium
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- sea cucumber
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- 230000036541 health Effects 0.000 title claims abstract description 49
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 238000004519 manufacturing process Methods 0.000 title description 2
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- 241001523681 Dendrobium Species 0.000 claims abstract description 43
- 239000000284 extract Substances 0.000 claims abstract description 38
- 239000000126 substance Substances 0.000 claims description 84
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 82
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 78
- 150000004676 glycans Chemical class 0.000 claims description 47
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- 239000000243 solution Substances 0.000 claims description 28
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- 239000012153 distilled water Substances 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 229960004329 metformin hydrochloride Drugs 0.000 description 4
- OETHQSJEHLVLGH-UHFFFAOYSA-N metformin hydrochloride Chemical compound Cl.CN(C)C(=N)N=C(N)N OETHQSJEHLVLGH-UHFFFAOYSA-N 0.000 description 4
- XZWYZXLIPXDOLR-UHFFFAOYSA-N metformin hydrochloride Natural products CN(C)C(=N)NC(N)=N XZWYZXLIPXDOLR-UHFFFAOYSA-N 0.000 description 4
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- 239000004375 Dextrin Substances 0.000 description 2
- VYZAHLCBVHPDDF-UHFFFAOYSA-N Dinitrochlorobenzene Chemical compound [O-][N+](=O)C1=CC=C(Cl)C([N+]([O-])=O)=C1 VYZAHLCBVHPDDF-UHFFFAOYSA-N 0.000 description 2
- 206010014025 Ear swelling Diseases 0.000 description 2
- 241000233855 Orchidaceae Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
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- HIMXGTXNXJYFGB-UHFFFAOYSA-N alloxan Chemical compound O=C1NC(=O)C(=O)C(=O)N1 HIMXGTXNXJYFGB-UHFFFAOYSA-N 0.000 description 2
- IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 description 2
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
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- CJDPJFRMHVXWPT-UHFFFAOYSA-N barium sulfide Chemical compound [S-2].[Ba+2] CJDPJFRMHVXWPT-UHFFFAOYSA-N 0.000 description 1
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- MAWWITJOQDJRJF-ADBICINLSA-M holothurin Chemical compound [Na+].O[C@@H]1[C@@H](OC)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O[C@H]3[C@@H](OC[C@H]([C@@H]3O)OS([O-])(=O)=O)O[C@@H]3C([C@H]4[C@](C=5[C@H]([C@@]6(CC[C@]7(O)[C@](C)([C@H]8OC(C)(C)CC8)OC(=O)[C@@]76[C@@H](O)C=5)C)CC4)(C)CC3)(C)C)O[C@@H]2C)O)O[C@H](C)[C@H]1O MAWWITJOQDJRJF-ADBICINLSA-M 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/616—Echinodermata, e.g. starfish, sea cucumbers or sea urchins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a health product and a preparation method thereof. The health product is prepared from sea cucumber and dendrobium as raw materials, is a pure natural biological extract product, is safe and non-toxic, has obvious effect, can achieve the effects of improving the immunity of organisms and reducing blood sugar by taking a proper amount every day, is taken persistently, contributes to the effects of strengthening physique and improving the health condition, is suitable for people with weakness, low immune function and high blood sugar, and is a good nutritional supplement for healthy people.
Description
The technical field is as follows:
the invention relates to the technical field of health-care food, in particular to a health-care product and a preparation method thereof.
Background art:
diabetes is a group of metabolic diseases characterized primarily by hyperglycemia. Hyperglycemia is caused by a defect in insulin secretion or an impaired biological action, or both. Hyperglycemia occurring in the long term of diabetes results in chronic damage to, and dysfunction of, various tissues, particularly the eyes, kidneys, heart, blood vessels, nerves. The immunity refers to the function of the body immune system to recognize self and foreign matters and eliminate antigenic foreign matters through immune response so as to maintain the physiological balance of the body. Immunity is a physiological function of the human body, and the human body recognizes "self" and "non-self" components depending on the function, thereby destroying and rejecting antigen substances entering the human body, or damaged cells and tumor cells generated by the human body itself, and the like, to maintain the health of the human body. With the progress of society and economy and the improvement of living standard of people, people begin to shift from heavy treatment to heavy prevention, which greatly promotes the research of medicines, foods and the like for reducing blood sugar and improving immunity.
Sea cucumber, belonging to Holothuroidea of Echinodermata, is a high-protein nutriment from ancient times, and has the health-preserving efficacies of mainly tonifying primordial qi, nourishing five internal organs and six internal organs and removing deficiency and damage in compendium of materia medica, historically called 'eight delicacies in sea', and is named as abalone and shark fin. Modern scientific researches find that the sea cucumber contains a plurality of important physiologically active substances, such as sea cucumber polypeptide, sea cucumber polysaccharide, sea cucumber saponin, sterol, fatty acid, toxin and the like, and the physiologically active substances have the effects of enhancing immunity, resisting tumors and blood coagulation, protecting nervous tissues, relieving pain, resisting fungi, reducing blood viscosity and the like.
Dendrobe, which is a collective name of fresh or dry stems of various plants in the genus of dendrobe in the family of orchidaceae, is one of the longest-history traditional Chinese medicine health preserving products, and has records in the earliest monograph in pharmacology in China, written in Shen nong Ben Cao Jing in east Han: dendrobium nobile, sweet and neutral in flavor. Mainly injures middle energizer, removes arthralgia, discharges qi, tonifies internal organs and consumptive disease, emaciation and strengthens yin. It can be taken for a long time to nourish stomach, lighten body and prolong life. One forest orchid (Yu Yun, one forbidden to live, see book, as black character) and one mountain valley. Ancient medical titles such as 'compendium of materia Medica' and 'famous medical miscellaneous records' are more highly advocated and have good medical value, and are popular among the famous medical titles in the past to be good medical medicines for 'tonifying internal organs and consumptive diseases, emaciation, yin strengthening, stomach thickening, weight reducing and life prolonging' and have high medicinal value.
The invention content is as follows:
the invention aims to provide a health-care product and a preparation method thereof.
The preparation method of the health care product comprises the following steps: mixing Stichopus japonicus saponins and herba Dendrobii polysaccharides uniformly to obtain health product.
Preferably, the step of uniformly mixing the holothurian saponin substances and the dendrobium polysaccharide substances is to uniformly mix the holothurian saponin substances and the dendrobium polysaccharide substances according to the mass ratio of 1: 1-3.
Preferably, the holothurian saponin substance is prepared by the following method: pulverizing dried sea cucumber or removing intestine from fresh sea cucumber, cleaning, drying, and pulverizing; adding ethanol water with volume fraction of 70-80% according to 5-15 times of the mass of the sea cucumber, carrying out reflux extraction at 50-90 ℃ for 5-10 hours, carrying out solid-liquid separation on the extracting solution, carrying out reduced pressure concentration on the supernatant to remove ethanol to obtain an extract, dispersing the extract in water, sequentially extracting with petroleum ether and n-butanol, and carrying out reduced pressure concentration and drying on the n-butanol extracting solution to obtain the sea cucumber saponin substance.
Preferably, the dendrobium polysaccharide substance is prepared by the following method: pulverizing dried stem of herba Dendrobii or cleaning fresh stem of herba Dendrobii, drying, and pulverizing; adding water which is 8-15 times of the mass of the dendrobium, performing reflux extraction at 60-95 ℃ for 1-8 hours, performing solid-liquid separation on the extracting solution, performing reduced pressure concentration on the supernatant to 1/5-1/10 of the original volume, adding ethanol until the volume fraction of the ethanol is 70-85%, standing for 12-24 hours, centrifuging to remove the supernatant, and drying the precipitate to obtain the dendrobium polysaccharide substance.
The sea cucumber is preferably stichopus japonicus, thelenota ananas, Holothuria scabra, Pachyrhizus japonicus, Panaxanthus japonicus or Pennisetum alnifolium.
The dendrobium is preferably dendrobium officinale.
The ethanol is preferably absolute ethanol.
The invention also aims to provide the health-care product with the functions of reducing blood sugar and improving immunity, which is prepared according to the preparation method.
The health product of the invention can also be used together with auxiliary materials acceptable in food or medicines. The adjuvant can be flavoring agent such as aspartame, sucrose, etc. or bulking agent such as starch, dextrin, etc. The content thereof is not strictly limited and is selected according to conventional methods, processes and requirements. Optionally adding filler such as starch and dextrin, optionally adding flavoring agent such as aspartame, and making into granule or other dosage forms by conventional method.
The method for taking the health care product comprises the following steps: the medicine is taken 2 times a day, 0.1-0.3 g each time, and can be taken for a long time.
The drug effect test of the health product comprises:
firstly, the efficacy test of the health product for improving immunity comprises the following steps:
selecting 50 healthy NIH male mice with the weight of 18-22 g, randomly dividing the healthy NIH male mice into 5 groups, namely a blank control group, a health-care product (the health-care product prepared in example 1) high and low dose group, a sea cucumber saponin substance (the sea cucumber saponin substance prepared in example 1) group and a dendrobium polysaccharide substance (the dendrobium polysaccharide substance prepared in example 1) group, wherein each group comprises 10 mice. The health-care product prepared in the gavage example 1 of the high-dose group of the health-care product has the dosage of 300 mg/kg; the dosage of the health care product prepared in the gavage example 1 in the low-dose group of the health care product is 100mg/kg, and the dosage of the holothurin saponin substances prepared in the gavage example 1 in the holothurin substance group is 300 mg/kg; dendrobe polysaccharide substance group dendrobe polysaccharide substance prepared in the gastric lavage example 1, the dosage is 300 mg/kg; the health care product prepared in example 1, the holothurian saponin substances and the dendrobium polysaccharide substances are respectively re-suspended by distilled water, each group is subjected to intragastric administration according to the weight of 0.2mL/10g, the continuous administration is carried out for 15 days, 1 time per day, and the blank control group is subjected to intragastric administration with distilled water with the same volume. The first administration was performed by dropping 50. mu.L/mouse abdominal skin (previously dehaired with barium sulfide) of 1% DNCB in acetone, and boosting once the next day. 1h after the last administration, 1% DNCB acetone solution is dropped on the two sides of the right ear of the mouse, 10 mu L of the acetone solution is dropped on each mouse, the mouse is killed at the cervical vertebra after 24h, the left ear and the right ear of the mouse are cut off, the ear piece at the same part is taken by an 8mm puncher, and the weight difference of the left ear and the right ear is regarded as swelling degree (mg). The greater the swelling, the stronger the reaction. The results are shown in Table 1.
Table 1: effects on delayed hypersensitivity of mouse skin: (
n＝10)
| Group of | Animal number (only) | Dosage (mg/kg) | Swelling degree of ear (mg) |
| Blank control group | 10 | — | 31.4±11.9cB |
| High dosage group of health products | 10 | 300 | 81.3±17.4aA |
| Low dosage group of health products | 10 | 100 | 67.6±22.3abA |
| Holothurian saponin substance | 10 | 300 | 56.8±27.1bA |
| Polysaccharides of herba Dendrobii | 10 | 300 | 62.2±17.1bA |
Note: the difference of different lower case letters in the same row is obvious (Duncan new compound difference method, P is less than 0.05), the difference of different upper case letters in the same row is obvious (Duncan new compound difference method, P is less than 0.01)
As can be seen from Table 1, the health care product of the invention can significantly increase the ear swelling degree of mice, which shows that the health care product of the invention can enhance the intensity of delayed hypersensitivity of mice and has the effect of significantly improving immunity. The ear swelling degree of the mice in the high-dose group of the health-care product is obviously higher than that of the mice in the holothurian saponin substance group and the dendrobium polysaccharide substance group, so that the health-care product disclosed by the invention has a synergistic effect by compounding the holothurian saponin substances and the dendrobium polysaccharide substances.
Secondly, the health care product of the invention has the function of reducing blood sugar of a diabetes model mouse:
150 healthy Kunming male mice are selected, the weight of the mice is 18-22 g, 10 healthy Kunming male mice are randomly selected as a blank control group after the mice are fed for 7 days, the rest 140 mice are used for constructing a diabetes model mouse, the mice are fasted (without water supply) for 24 hours before modeling, alloxan is injected into the abdominal cavity at one time for 160mg/kg, the mice are fasted for 4 hours after 5 days, eyeball blood is taken for detecting fasting blood glucose, and the fasting blood glucose value is higher than 11.1mmol/L to achieve successful modeling. Selecting 60 diabetes model mice, randomly dividing into 6 groups: a model control group, a positive (metformin hydrochloride) control group, two dosage groups of a high dosage group and a low dosage group of a health care product (the health care product prepared in example 1), a holothurin saponin substance group (the holothurin saponin substance prepared in example 1) and a dendrobium polysaccharide substance group (the polysaccharide dendrobium substance prepared in example 1), wherein each group contains 10 capsules. The health-care product prepared in the gavage example 1 of the high-dose group of the health-care product has the dosage of 300 mg/kg; the health-care product prepared in the gastric lavage group of example 1 is administrated at the dosage of 100 mg/kg; the holothurian saponin substance group is the holothurian saponin substance prepared in the gastric lavage example 1, and the dosage is 300 mg/kg; dendrobe polysaccharide substance group dendrobe polysaccharide substance prepared in the gastric lavage example 1, the dosage is 300 mg/kg; the positive control group is infused with metformin hydrochloride, and the dosage is 500 mg/kg; the health care product prepared in example 1, the holothurian saponin substances and the dendrobium polysaccharide substances are respectively resuspended by distilled water, each group is subjected to intragastric administration according to the weight of 0.2mL/10g, the continuous administration is carried out for 15 days, 1 time per day, and the distilled water with the same volume as the intragastric administration is carried out on a blank control group and a model control group. Fasted for 24h, weighed and fasting blood glucose measured. The results are shown in tables 2 and 3.
Table 2: the influence of the health product of the invention on the body weight of a diabetes model mouse (
n＝10)
| Group of | Animal number (only) | Dosage (mg/kg) | Initial weight (g) | Final weight (g) |
| Blank control group | 10 | — | 23.2±1.7 | 28.5±2.1aA |
| Model control group | 10 | — | 20.6±1.4 | 17.8±2.2dD |
| Positive control group | 10 | 500 | 20.4±1.7 | 21.7±2.5bcBC |
| High dosage group of health products | 10 | 300 | 20.8±1.5 | 23.4±2.4bB |
| Low dosage group of health products | 10 | 100 | 20.5±1.6 | 21.6±2.2bcBC |
| Holothurian saponin substance group | 10 | 300 | 20.6±1.5 | 20.4±2.2cCD |
| Polysaccharide group of dendrobe | 10 | 300 | 20.8±1.6 | 20.8±2.1cBC |
Note: the difference of different lower case letters in the same row is obvious (Duncan new compound difference method, P is less than 0.05), the difference of different upper case letters in the same row is obvious (Duncan new compound difference method, P is less than 0.01)
As can be seen from Table 2, after administration for 15 days by gavage, the weights of the mice in the positive control group, the mice in the high and low dose groups of the health care products, the mice in the holothurian saponin substance group and the dendrobium polysaccharide substance group are all significantly higher than those of the mice in the model control group, and the weights of the mice in the high dose group of the health care products are significantly higher than those of the mice in the holothurian saponin substance group and the dendrobium polysaccharide substance group. Transverse comparison (weight before and after experiment) shows that the weight of the mice in the blank control group is increased, the weight of the mice in the model control group is reduced, which indicates that the weight of the mice in the diabetes model is increased negatively, and the weights of the mice in the positive control group, the mice in the health product high and low dose groups, the mice in the sea cucumber saponin substance group and the mice in the dendrobe polysaccharide substance group are all obviously higher than those of the mice in the model control group and lower than those of the mice in the blank control group, which indicates that the health product can slow down the weight reduction trend of the mice in the diabetes model. Therefore, the health care product has a function of slowing down the weight loss of a diabetes model mouse, and the effect is better than that of metformin hydrochloride.
Table 3: the influence of the health product on the blood sugar of a diabetes model mouse (
n＝10)
Note: the difference of different lower case letters in the same row is obvious (Duncan new compound difference method, P is less than 0.05), the difference of different upper case letters in the same row is obvious (Duncan new compound difference method, P is less than 0.01)
As can be seen from Table 3, the blood glucose values of the mice of the positive control group, the mice of the high-dose group of the health care product, the mice of the low-dose group of the health care product, the mice of the holothurian saponin substance group and the mice of the dendrobium polysaccharide substance group are all significantly lower than those of the mice of the model control group after the administration for 15 days by intragastric administration. Transverse comparison (blood sugar values before and after experiments) shows that after the administration for 15 days by gastric lavage, the blood sugar values of mice in the positive control group, the health-care product high-dose group, the health-care product low-dose group, the holothurin saponin substance group and the dendrobium polysaccharide substance group are all obviously reduced, and the blood sugar value of the mice in the health-care product high-dose group is obviously lower than that of the mice in the positive control group, the holothurin saponin substance group and the dendrobium polysaccharide substance group. Therefore, the health care product has stronger hypoglycemic effect and better effect than metformin hydrochloride. The health product has the blood sugar reducing effect obviously higher than that of single use (dendrobium polysaccharide substances or sea cucumber saponin substances), and has synergistic effect by compounding the sea cucumber saponin substances and the dendrobium polysaccharide substances.
The health product is prepared from sea cucumber and dendrobium as raw materials, is a pure natural biological extract product, is safe and non-toxic, has obvious effect, can achieve the effects of improving the immunity of organisms and reducing blood sugar by taking a proper amount every day, is taken persistently, contributes to the effects of strengthening physique and improving the health condition, is suitable for people with weakness, low immune function and high blood sugar, and is a good nutritional supplement for healthy people.
The specific implementation mode is as follows:
the following examples are further illustrative of the present invention and are not intended to be limiting thereof.
Example 1:
removing intestines of fresh stichopus japonicus, cleaning, drying, crushing, putting 10kg of fresh stichopus japonicus into 120kg of ethanol aqueous solution with 75% of volume fraction, performing reflux extraction at 80 ℃ for 8 hours, centrifuging the extract at 6000rpm for 10min, separating and removing residues, performing reduced pressure concentration on the supernatant to remove ethanol to obtain an extract, dispersing the extract into 5L of water, firstly extracting with 5L of petroleum ether, extracting the extracted water phase with 5L of n-butyl alcohol, performing reduced pressure concentration on the n-butyl alcohol extract to remove n-butyl alcohol to obtain an extract, and performing vacuum freeze drying to obtain the holothurian saponins; cleaning fresh dendrobium officinale stems, drying, crushing, putting 10kg of the fresh dendrobium officinale stems into 120kg of water, carrying out reflux extraction at 80 ℃ for 5 hours, centrifuging the extracting solution at 6000rpm for 10 minutes, separating and removing residues, concentrating the supernatant into 1/6 with the original volume under reduced pressure, adding absolute ethyl alcohol until the volume fraction of the ethyl alcohol is 80%, standing at 4 ℃ for 12 hours, centrifuging at 5000rpm for 10 minutes, removing the supernatant, and drying the precipitate to obtain dendrobium polysaccharide substances. Uniformly mixing the sea cucumber saponin substances and the dendrobium polysaccharide substances according to the mass ratio of 1:1 to obtain the health-care product. Animal experiments prove that the health care product has the functions of reducing blood sugar and improving the immunity of the organism.
Example 2:
removing intestines of fresh white sea cucumber, cleaning, drying, crushing, putting 10kg into 50kg of ethanol water solution with volume fraction of 70%, refluxing and extracting at 50 ℃ for 10 hours, centrifuging the extracting solution at 6000rpm for 10min, separating and removing residues, performing reduced pressure concentration on the supernatant to remove ethanol to obtain an extract, dispersing the extract into 5L of water, firstly extracting with 5L of petroleum ether, extracting the extracted water phase with 5L of n-butyl alcohol, performing reduced pressure concentration on the n-butyl alcohol extracting solution to remove n-butyl alcohol to obtain an extract, and performing vacuum freeze drying to obtain the sea cucumber saponins; cleaning fresh dendrobium officinale stems, drying, crushing, putting 10kg of the fresh dendrobium officinale stems into 80kg of water, carrying out reflux extraction at 60 ℃ for 8 hours, centrifuging the extracting solution at 6000rpm for 10 minutes, separating and removing residues, concentrating the supernatant fluid under reduced pressure to 1/5 of the original volume, adding absolute ethyl alcohol until the volume fraction of the ethyl alcohol is 70%, standing at 4 ℃ for 15 hours, centrifuging at 5000rpm for 10 minutes, removing the supernatant fluid, and drying the precipitate to obtain dendrobium polysaccharide substances. Uniformly mixing the sea cucumber saponin substances and the dendrobium polysaccharide substances according to the mass ratio of 1:2 to obtain the health-care product. Animal experiments prove that the health care product has the functions of reducing blood sugar and improving the immunity of the organism.
Example 3:
removing intestines of fresh thelenota ananas, cleaning, drying, crushing, putting 10kg of the fresh thelenota ananas into 150kg of ethanol water solution with volume fraction of 80%, refluxing and extracting for 5 hours at 90 ℃, centrifuging the extracting solution at 6000rpm for 10min, separating and removing residues, performing reduced pressure concentration on the supernatant to remove ethanol to obtain an extract, dispersing the extract into 5L of water, extracting with 5L of petroleum ether, extracting the extracted water phase with 5L of n-butyl alcohol, performing reduced pressure concentration on the n-butyl alcohol extracting solution to remove the n-butyl alcohol to obtain an extract, and performing vacuum freeze drying to obtain the holothurian saponins; cleaning fresh dendrobium officinale stems, drying, crushing, putting 10kg of the fresh dendrobium officinale stems into 150kg of water, carrying out reflux extraction at 95 ℃ for 1 hour, centrifuging the extracting solution at 6000rpm for 10min, separating and removing residues, concentrating the supernatant into 1/10 of the original volume under reduced pressure, adding absolute ethyl alcohol until the volume fraction of the ethyl alcohol is 85%, standing at 4 ℃ for 24 hours, centrifuging at 5000rpm for 10min, removing the supernatant, and drying the precipitate to obtain dendrobium polysaccharide substances. Uniformly mixing the sea cucumber saponin substances and the dendrobium polysaccharide substances according to the mass ratio of 1:3 to obtain the health-care product. Animal experiments prove that the health care product has the functions of reducing blood sugar and improving the immunity of the organism.
Example 4:
crushing dry holothuria scabra, putting 10kg of the dry holothuria scabra into 120kg of ethanol water solution with 75% of volume fraction, performing reflux extraction at 80 ℃ for 8 hours, centrifuging the extracting solution at 6000rpm for 10min, removing residues, performing reduced pressure concentration on the supernatant to remove ethanol to obtain an extract, dispersing the extract into 5L of water, extracting with 5L of petroleum ether, extracting the extracted water phase with 5L of n-butyl alcohol, performing reduced pressure concentration on the n-butyl alcohol extracting solution to remove n-butyl alcohol to obtain an extract, and performing vacuum freeze drying to obtain holothuria saponins; pulverizing dry stem of herba Dendrobii, placing 10kg into 120kg water, reflux extracting at 80 deg.C for 5 hr, centrifuging the extractive solution at 6000rpm for 10min to remove residue, concentrating the supernatant under reduced pressure to 1/6 of original volume, adding anhydrous ethanol until ethanol volume fraction is 80%, standing at 4 deg.C for 12 hr, centrifuging at 5000rpm for 10min to remove supernatant, and drying the precipitate to obtain herba Dendrobii polysaccharide substance. Uniformly mixing the sea cucumber saponin substances and the dendrobium polysaccharide substances according to the mass ratio of 1:1 to obtain the health-care product. Animal experiments prove that the health care product has the functions of reducing blood sugar and improving the immunity of the organism.
Example 5:
removing intestines of fresh radix panacis majoris, cleaning, drying, crushing, putting 10kg of the fresh radix panacis majoris into 120kg of ethanol water with 75% of volume fraction, performing reflux extraction at 80 ℃ for 8 hours, centrifuging the extracting solution at 6000rpm for 10min, separating and removing residues, performing reduced pressure concentration on the supernatant to remove ethanol to obtain an extract, dispersing the extract into 5L of water, firstly extracting the extract with 5L of petroleum ether, extracting the extracted water phase with 5L of n-butyl alcohol, performing reduced pressure concentration on the n-butyl alcohol extracting solution to remove n-butyl alcohol to obtain an extract, and performing vacuum freeze drying to obtain a holothurian saponin substance; cleaning fresh dendrobium officinale stems, drying, crushing, putting 10kg of the fresh dendrobium officinale stems into 120kg of water, carrying out reflux extraction at 80 ℃ for 5 hours, centrifuging the extracting solution at 6000rpm for 10 minutes, separating and removing residues, concentrating the supernatant into 1/6 with the original volume under reduced pressure, adding absolute ethyl alcohol until the volume fraction of the ethyl alcohol is 80%, standing at 4 ℃ for 12 hours, centrifuging at 5000rpm for 10 minutes, removing the supernatant, and drying the precipitate to obtain dendrobium polysaccharide substances. Uniformly mixing the sea cucumber saponin substances and the dendrobium polysaccharide substances according to the mass ratio of 1:1 to obtain the health-care product. Animal experiments prove that the health care product has the functions of reducing blood sugar and improving the immunity of the organism.
Example 6:
removing intestines of fresh stichopus japonicus, cleaning, drying, crushing, putting 10kg of fresh stichopus japonicus into 120kg of ethanol aqueous solution with 75% of volume fraction, performing reflux extraction at 80 ℃ for 8 hours, centrifuging the extract at 6000rpm for 10min, separating and removing residues, performing reduced pressure concentration on the supernatant to remove ethanol to obtain an extract, dispersing the extract into 5L of water, firstly extracting with 5L of petroleum ether, extracting the extracted water phase with 5L of n-butyl alcohol, performing reduced pressure concentration on the n-butyl alcohol extract to remove n-butyl alcohol to obtain an extract, and performing vacuum freeze drying to obtain the holothurian saponin substances; cleaning fresh dendrobium officinale stems, drying, crushing, putting 10kg of the fresh dendrobium officinale stems into 120kg of water, carrying out reflux extraction at 80 ℃ for 5 hours, centrifuging the extracting solution at 6000rpm for 10 minutes, separating and removing residues, concentrating the supernatant into 1/6 with the original volume under reduced pressure, adding absolute ethyl alcohol until the volume fraction of the ethyl alcohol is 80%, standing at 4 ℃ for 12 hours, centrifuging at 5000rpm for 10 minutes, removing the supernatant, and drying the precipitate to obtain dendrobium polysaccharide substances. Uniformly mixing the sea cucumber saponin substances and the dendrobium polysaccharide substances according to the mass ratio of 1:1 to obtain the health-care product. Animal experiments prove that the health care product has the functions of reducing blood sugar and improving the immunity of the organism.
Example 7:
removing intestines of fresh acaudina molpadioides, cleaning, drying, crushing, putting 10kg of fresh acaudina molpadioides in 120kg of ethanol aqueous solution with volume fraction of 75%, performing reflux extraction at 80 ℃ for 8 hours, centrifuging the extract at 6000rpm for 10min, separating and removing residues, performing reduced pressure concentration on the supernatant to remove ethanol to obtain an extract, dispersing the extract in 5L of water, extracting with 5L of petroleum ether, extracting the extracted water phase with 5L of n-butyl alcohol, performing reduced pressure concentration on the n-butyl alcohol extract to remove n-butyl alcohol to obtain an extract, and performing vacuum freeze drying to obtain a sea cucumber saponin substance; cleaning fresh dendrobium officinale stems, drying, crushing, putting 10kg of the fresh dendrobium officinale stems into 120kg of water, carrying out reflux extraction at 80 ℃ for 5 hours, centrifuging the extracting solution at 6000rpm for 10 minutes, separating and removing residues, concentrating the supernatant into 1/6 with the original volume under reduced pressure, adding absolute ethyl alcohol until the volume fraction of the ethyl alcohol is 80%, standing at 4 ℃ for 12 hours, centrifuging at 5000rpm for 10 minutes, removing the supernatant, and drying the precipitate to obtain dendrobium polysaccharide substances. Uniformly mixing the sea cucumber saponin substances and the dendrobium polysaccharide substances according to the mass ratio of 1:1 to obtain the health-care product. Animal experiments prove that the health care product has the functions of reducing blood sugar and improving the immunity of the organism.
Example 8:
removing intestines of fresh black stichopus japonicus, cleaning, drying, crushing, putting 10kg of the fresh black stichopus japonicus into 120kg of ethanol water solution with 75% of volume fraction, performing reflux extraction at 80 ℃ for 8 hours, centrifuging the extracting solution at 6000rpm for 10min, separating and removing residues, performing reduced pressure concentration on the supernatant to remove ethanol to obtain an extract, dispersing the extract into 5L of water, firstly extracting with 5L of petroleum ether, extracting the extracted water phase with 5L of n-butyl alcohol, performing reduced pressure concentration on the n-butyl alcohol extracting solution to remove n-butyl alcohol to obtain an extract, and performing vacuum freeze drying to obtain the holothurian saponin substances; cleaning fresh dendrobium officinale stems, drying, crushing, putting 10kg of the fresh dendrobium officinale stems into 120kg of water, carrying out reflux extraction at 80 ℃ for 5 hours, centrifuging the extracting solution at 6000rpm for 10 minutes, separating and removing residues, concentrating the supernatant into 1/6 with the original volume under reduced pressure, adding absolute ethyl alcohol until the volume fraction of the ethyl alcohol is 80%, standing at 4 ℃ for 12 hours, centrifuging at 5000rpm for 10 minutes, removing the supernatant, and drying the precipitate to obtain dendrobium polysaccharide substances. Uniformly mixing the sea cucumber saponin substances and the dendrobium polysaccharide substances according to the mass ratio of 1:1 to obtain the health-care product. Animal experiments prove that the health care product has the functions of reducing blood sugar and improving the immunity of the organism.
Claims (5)
1. A preparation method of a health product is characterized in that holothurian saponin substances and dendrobium polysaccharide substances are uniformly mixed according to the mass ratio of 1: 1-3;
the holothurian saponin substance is prepared by the following method: pulverizing dried sea cucumber or removing intestine from fresh sea cucumber, cleaning, drying, and pulverizing; adding 70-80% ethanol aqueous solution by volume fraction according to 5-15 times of the mass of the sea cucumber, carrying out reflux extraction at 50-90 ℃ for 5-10 hours, carrying out solid-liquid separation on the extract, carrying out reduced pressure concentration on the supernatant to remove ethanol to obtain an extract, dispersing the extract in water, sequentially extracting with petroleum ether and n-butanol, and carrying out reduced pressure concentration and drying on the n-butanol extract to obtain the sea cucumber saponins;
the dendrobium polysaccharide substance is prepared by the following method: pulverizing dried stem of herba Dendrobii or cleaning fresh stem of herba Dendrobii, drying, and pulverizing; adding water which is 8-15 times of the mass of dendrobium, performing reflux extraction at 60-95 ℃ for 1-8 hours, performing solid-liquid separation on the extracting solution, performing reduced pressure concentration on the supernatant to 1/5-1/10 of the original volume, adding ethanol until the volume fraction of the ethanol is 70-85%, standing for 12-24 hours, centrifuging to remove the supernatant, and drying the precipitate to obtain the dendrobium polysaccharide substance.
2. The method according to claim 1, wherein the sea cucumber is Stichopus japonicus selenka, Thelenota ananas, Holothuria scabra, Pachyrhizus hainanensis, Panax quinquefolium or Panax japonicus selenka.
3. The method according to claim 1, wherein the dendrobium is dendrobium officinale.
4. The method according to claim 1, wherein the ethanol is absolute ethanol.
5. A health product prepared by the preparation method of any one of claims 1 to 4.
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