CN109430783B - Enzymolysis method of pollen - Google Patents

Abstract

The invention discloses an enzymolysis method of pollen, which comprises the following steps: s1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding into a 100L reaction kettle, adding 80Kg of purified water, 100g of cellulase and 100g of alpha-amylase, heating to 40 +/-5 ℃, reacting for 5 +/-0.5 h, heating to 80 ℃, adding 100g of protease, and reacting for 12h. S2, filtering: after the reaction is finished, filtering while the solution is hot, and taking the filtrate, S3, concentrating: concentrating under reduced pressure to density of about 1.1, and discharging extract. The method has simple and safe process, and does not use other organic reagents compared with the traditional method of adopting enzymolysis water extraction; the invention has more complete enzymolysis, and the content of the small molecular peptide after the enzymolysis is more than 30 percent, thereby greatly improving the absorption effect of the human body and being suitable for popularization and use.

Description

Enzymolysis method of pollen

Technical Field

The invention relates to an enzymolysis method, in particular to an enzymolysis method of pollen.

Background

Pollen (pollen pini, corn pollen and rape pollen) is a natural resource with abundant nutrition, but because the pollen wall is compact, the nutrient substances are contained in the pollen wall, are not easy to dissociate and are absorbed and utilized by human bodies, and can not be fully absorbed and utilized when being directly used or the wall breaking is insufficient, such as mechanical wall breaking, the absorption and utilization of the pollen can be insufficient, and the yield is 5 percent when a non-enzymatic technology (direct water extraction) is directly adopted, and the yield of extract is extremely low, so that an enzymatic hydrolysis method for the pollen is designed to solve the problems.

Disclosure of Invention

The invention aims to solve the defects in the prior art and provides an enzymolysis method of pollen.

In order to achieve the purpose, the invention adopts the following technical scheme:

an enzymolysis method of pollen comprises the following steps:

s1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding into a 100L reaction kettle, adding 80Kg of purified water, 100g of cellulase and 100g of alpha-amylase, heating to 40 +/-5 ℃, reacting for 5 +/-0.5 h, heating to 80 ℃, adding 100g of protease, and reacting for 12h;

s2, filtering: after the reaction is finished, filtering while the solution is hot, and taking filtrate;

s3, concentrating: concentrating under reduced pressure to density of about 1.1, and discharging extract;

s4, spray drying: opening a steam valve to ensure that the steam pressure reaches 0.1MPa, and keeping the temperature in the spray drying tank at 110-120 ℃; opening a compressed air valve and a peristaltic pump simultaneously to spray the liquid medicine into the tank body in a mist shape, and collecting dry powder to obtain the medicine;

s5, mixing and granulating: and (3) mixing the extract powder: beta cyclodextrin is as follows: purified water was prepared as 1:1:0.1, mixing for 20min, mixing uniformly, performing swing granulation in a swing granulator, and sieving with a 20-mesh sieve;

s6, drying: drying in a hot air circulation oven at 80 +/-5 ℃ for 24h;

s7, inner packaging: and (5) carrying out inner packaging by adopting a particle racking machine to obtain a finished product.

Preferably, the step of S1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding into a 100L reaction kettle, adding 80Kg of purified water and 100g of alpha-amylase, heating to 40 +/-5 ℃, and reacting for 5 +/-0.5 h.

Preferably, the step of S1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding into a 100L reaction kettle, adding 80Kg of purified water, 100g of alpha-amylase and 100g of cellulase, heating to 40 +/-5 ℃, and reacting for 5 +/-0.5 h.

Preferably, the step of S1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding into a 100L reaction kettle, adding 80Kg of purified water and 100g of alpha-amylase, heating to 40 +/-5 ℃, reacting for 5h +/-0.5 h, heating to 80 ℃, adding 100g of protease, and reacting for 12h.

Preferably, the step of S1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding into a 100L reaction kettle, adding 80Kg of purified water, 100 papain and 100g of alpha-amylase, heating to 40 +/-5 ℃, and reacting for 5 +/-0.5 h.

Preferably, the step of S1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the cell walls, adding into a 100L reaction kettle, adding 80Kg of purified water and 100g of cellulase, heating to 40 +/-5 ℃, reacting for 5 +/-0.5 h, heating to 80 ℃, adding 100g of protease, and reacting for 12h.

Preferably, the step S1 and the step of enzymatic hydrolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding into a 100L reaction kettle, adding 80Kg of purified water, 100g of cellulase, 100g of alpha-amylase and 100g of papain, heating to 40 +/-5 ℃, reacting for 5h +/-0.5 h, heating to 80 ℃, then adding 100g of protease, and reacting for 12h.

Compared with the prior art, the invention has the beneficial effects that:

the method for the enzymolysis of the pollen provided by the invention has simple and safe process, and does not use other organic reagents compared with the traditional method adopting enzymolysis water extraction; the invention has more complete enzymolysis, and the content of the micromolecule peptide after the enzymolysis is more than 30 percent, thereby greatly improving the extract yield and being suitable for popularization and use.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.

Example one

An enzymolysis method of pollen comprises the following steps:

s1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding into a 100L reaction kettle, adding 80Kg of purified water, 100g of cellulase and 100g of alpha-amylase, heating to 40 +/-5 ℃, reacting for 5 +/-0.5 h, heating to 80 ℃, adding 100g of protease, and reacting for 12h;

s2, filtering: after the reaction is finished, filtering while the solution is hot, and taking filtrate;

s3, concentrating: concentrating under reduced pressure to density of about 1.1, and discharging extract;

s4, spray drying: opening a steam valve to ensure that the steam pressure reaches 0.1MPa, and keeping the temperature in the spray drying tank at 110-120 ℃; opening a compressed air valve and a peristaltic pump simultaneously to spray the liquid medicine into the tank body in a mist shape, and collecting dry powder to obtain the medicine;

s5, mixing and granulating: and (3) mixing the extract powder: beta cyclodextrin is prepared by the following steps: purified water was prepared as 1:1:0.1, mixing for 20min, mixing uniformly, performing swing granulation in a swing granulator, and sieving with a 20-mesh sieve;

s6, drying: drying in a hot air circulation oven at 80 +/-5 ℃ for 24h;

s7, inner packaging: carrying out inner packaging by adopting a particle subpackaging machine to obtain a finished product;

example two

S1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding the pollen pini into a 100L reaction kettle, adding 80Kg of purified water and 100g of alpha-amylase, heating to 40 +/-5 ℃, and reacting for 5 +/-0.5 h;

s2, filtering: after the reaction is finished, filtering while the solution is hot, and taking filtrate;

s3, concentrating: concentrating under reduced pressure to density of about 1.1, and discharging extract;

s4, spray drying: opening a steam valve to ensure that the steam pressure reaches 0.1MPa, and keeping the temperature in the spray drying tank at 110-120 ℃; opening a compressed air valve, simultaneously opening a peristaltic pump to spray the liquid medicine into the tank body in a mist shape, and collecting dry powder to obtain the medicine;

s5, mixing and granulating: and (3) mixing the extract powder: beta cyclodextrin is as follows: purified water was prepared as 1:1:0.1, mixing for 20min, mixing uniformly, performing swing granulation in a swing granulator, and sieving with a 20-mesh sieve;

s6, drying: drying for 24 hours at the temperature of 80 +/-5 ℃ in a hot air circulation drying oven;

s7, inner packaging: and (5) carrying out inner packing by adopting a particle packing machine to obtain a finished product.

EXAMPLE III

S1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding into a 100L reaction kettle, adding 80Kg of purified water, 100g of alpha-amylase and 100g of cellulase, heating to 40 +/-5 ℃, and reacting for 5 +/-0.5 h;

s2, filtering: after the reaction is finished, filtering while the solution is hot, and taking filtrate;

s3, concentrating: concentrating under reduced pressure to density of about 1.1, and discharging extract;

s4, spray drying: opening a steam valve to ensure that the steam pressure reaches 0.1MPa, and keeping the temperature in the spray drying tank at 110-120 ℃; opening a compressed air valve and a peristaltic pump simultaneously to spray the liquid medicine into the tank body in a mist shape, and collecting dry powder to obtain the medicine;

s5, mixing and granulating: and (3) mixing the extract powder: beta cyclodextrin is as follows: purified water was prepared as 1:1:0.1, mixing for 20min, mixing uniformly, performing swing granulation in a swing granulator, and sieving with a 20-mesh sieve;

s6, drying: drying in a hot air circulation oven at 80 +/-5 ℃ for 24h;

s7, inner packaging: and (5) carrying out inner packing by adopting a particle packing machine to obtain a finished product.

Example four

S1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding the pollen pini into a 100L reaction kettle, adding 80Kg of purified water and 100g of alpha-amylase, heating to 40 +/-5 ℃, reacting for 5 +/-0.5 h, heating to 80 ℃, adding 100g of protease, and reacting for 12h;

s2, filtering: after the reaction is finished, filtering while the solution is hot, and taking filtrate;

s3, concentrating: concentrating under reduced pressure to density of about 1.1, and discharging extract;

s4, spray drying: opening a steam valve to ensure that the steam pressure reaches 0.1MPa, and keeping the temperature in the spray drying tank at 110-120 ℃; opening a compressed air valve and a peristaltic pump simultaneously to spray the liquid medicine into the tank body in a mist shape, and collecting dry powder to obtain the medicine;

s5, mixing and granulating: and (3) mixing the extract powder: beta cyclodextrin is as follows: purified water was prepared as 1:1:0.1 mixing for 20min, mixing uniformly, performing swing granulation in a swing granulator, and sieving with a 20-mesh sieve;

s6, drying: drying in a hot air circulation oven at 80 +/-5 ℃ for 24h;

s7, inner packaging: and (5) carrying out inner packaging by adopting a particle racking machine to obtain a finished product.

EXAMPLE five

S1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding into a 100L reaction kettle, adding 80Kg of purified water, 100 papain and 100g of alpha-amylase, heating to 40 +/-5 ℃, and reacting for 5 +/-0.5 h;

s2, filtering: after the reaction is finished, filtering while the solution is hot, and taking filtrate;

s3, concentrating: concentrating under reduced pressure to density of about 1.1, and discharging extract;

s4, spray drying: opening a steam valve to ensure that the steam pressure reaches 0.1MPa, and keeping the temperature in the spray drying tank at 110-120 ℃; opening a compressed air valve and a peristaltic pump simultaneously to spray the liquid medicine into the tank body in a mist shape, and collecting dry powder to obtain the medicine;

s5, mixing and granulating: and (3) mixing the extract powder: beta cyclodextrin is prepared by the following steps: purified water was prepared as 1:1:0.1, mixing for 20min, mixing uniformly, performing swing granulation in a swing granulator, and sieving with a 20-mesh sieve;

s6, drying: drying in a hot air circulation oven at 80 +/-5 ℃ for 24h;

s7, inner packaging: and (5) carrying out inner packing by adopting a particle packing machine to obtain a finished product.

EXAMPLE six

S1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding the pollen pini into a 100L reaction kettle, adding 80Kg of purified water and 100g of cellulase, heating to 40 +/-5 ℃, reacting for 5 +/-0.5 h, heating to 80 ℃, adding 100g of protease, and reacting for 12h;

s2, filtering: after the reaction is finished, filtering while the solution is hot, and taking filtrate;

s3, concentrating: concentrating under reduced pressure to density of about 1.1, and discharging extract;

s4, spray drying: opening a steam valve to ensure that the steam pressure reaches 0.1MPa, and keeping the temperature in the spray drying tank at 110-120 ℃; opening a compressed air valve and a peristaltic pump simultaneously to spray the liquid medicine into the tank body in a mist shape, and collecting dry powder to obtain the medicine;

s5, mixing and granulating: and (3) mixing the extract powder: beta cyclodextrin is prepared by the following steps: purified water was prepared as 1:1:0.1, mixing for 20min, mixing uniformly, performing swing granulation in a swing granulator, and sieving with a 20-mesh sieve;

s6, drying: drying in a hot air circulation oven at 80 +/-5 ℃ for 24h;

s7, inner packaging: and (5) carrying out inner packing by adopting a particle packing machine to obtain a finished product.

EXAMPLE seven

S1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding into a 100L reaction kettle, adding 80Kg of purified water, 100g of cellulase, 100g of alpha-amylase and 100g of papain, heating to 40 +/-5 ℃, reacting for 5h +/-0.5 h, heating to 80 ℃, then adding 100g of protease, and reacting for 12h;

s2, filtering: after the reaction is finished, filtering while the solution is hot, and taking filtrate;

s3, concentrating: concentrating under reduced pressure to density of about 1.1, and discharging extract;

s4, spray drying: opening a steam valve to ensure that the steam pressure reaches 0.1MPa, and keeping the temperature in the spray drying tank at 110-120 ℃; opening a compressed air valve and a peristaltic pump simultaneously to spray the liquid medicine into the tank body in a mist shape, and collecting dry powder to obtain the medicine;

s5, mixing and granulating: and (3) mixing the extract powder: beta cyclodextrin is as follows: purified water was prepared as 1:1:0.1, mixing for 20min, mixing uniformly, performing swing granulation in a swing granulator, and sieving with a 20-mesh sieve;

s6, drying: drying in a hot air circulation oven at 80 +/-5 ℃ for 24h;

s7, inner packaging: and (5) carrying out inner packaging by adopting a particle racking machine to obtain a finished product.

The following table is obtained from examples one to seven:

examples	Yield of
		Example one	30%
Example two	12%
		EXAMPLE III	26%
Example four	18%
		EXAMPLE five	20%
EXAMPLE six	24%
		EXAMPLE seven	40%

The table shows that the optimal scheme of the invention is the seventh embodiment, amylase, cellulase, protease and papain are adopted, and the yield of the extract is 40%.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (1)

1. An enzymolysis method of pollen is characterized by comprising the following steps:

s1, enzymolysis: taking 5Kg of pollen pini, 10Kg of corn pollen and 5Kg of rape pollen, breaking the walls, adding into a 100L reaction kettle, adding 80Kg of purified water, 100g of cellulase, 100g of alpha-amylase and 100g of papain, heating to 40 +/-5 ℃, reacting for 5h +/-0.5 h, heating to 80 ℃, then adding 100g of protease, and reacting for 12h;

s2, filtering: after the reaction is finished, filtering while the solution is hot, and taking filtrate;

s3, concentrating: concentrating under reduced pressure to density of 1.1, and discharging extract;

s4, spray drying: opening a steam valve to ensure that the steam pressure reaches 0.1MPa, and keeping the temperature in the spray drying tank at 110-120 ℃; opening a compressed air valve and a peristaltic pump simultaneously to spray the liquid medicine into the tank body in a mist shape, and collecting dry powder to obtain the medicine;

s5, mixing and granulating: and (3) mixing the extract powder: beta cyclodextrin is as follows: purified water was prepared as 1:1:0.1, mixing for 20min, mixing uniformly, performing swing granulation in a swing granulator, and sieving with a 20-mesh sieve;

s6, drying: drying for 24 hours at the temperature of 80 +/-5 ℃ in a hot air circulation drying oven;

s7, inner packaging: and (5) carrying out inner packaging by adopting a particle racking machine to obtain a finished product.