CN87106139A - Method for producing pollen essence - Google Patents
Method for producing pollen essence Download PDFInfo
- Publication number
- CN87106139A CN87106139A CN87106139.2A CN87106139A CN87106139A CN 87106139 A CN87106139 A CN 87106139A CN 87106139 A CN87106139 A CN 87106139A CN 87106139 A CN87106139 A CN 87106139A
- Authority
- CN
- China
- Prior art keywords
- pollen
- concentrate
- filter
- add
- hour
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 14
- 238000000605 extraction Methods 0.000 claims abstract description 8
- 239000000284 extract Substances 0.000 claims abstract description 6
- 239000002253 acid Substances 0.000 claims abstract description 5
- 239000012141 concentrate Substances 0.000 claims description 20
- 239000002904 solvent Substances 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 7
- 239000000706 filtrate Substances 0.000 claims description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 238000005984 hydrogenation reaction Methods 0.000 claims description 5
- CHWRSCGUEQEHOH-UHFFFAOYSA-N potassium oxide Chemical compound [O-2].[K+].[K+] CHWRSCGUEQEHOH-UHFFFAOYSA-N 0.000 claims description 5
- 229910001950 potassium oxide Inorganic materials 0.000 claims description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- 239000003960 organic solvent Substances 0.000 claims description 3
- 238000007127 saponification reaction Methods 0.000 claims description 3
- 241000256844 Apis mellifera Species 0.000 claims description 2
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 claims description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 241000220259 Raphanus Species 0.000 claims 1
- -1 filter Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 5
- 238000002156 mixing Methods 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 239000007787 solid Substances 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 abstract 1
- 238000012423 maintenance Methods 0.000 abstract 1
- 230000035764 nutrition Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 5
- 239000004480 active ingredient Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 239000011149 active material Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 238000007670 refining Methods 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229930191515 Pollenin Natural products 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 230000007198 pollen germination Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Landscapes
- Jellies, Jams, And Syrups (AREA)
Abstract
A process for preparing pollen extract includes such steps as adding water to pollen, enzymolyzing at 35-55 deg.C to break the wall of pollen shell, sequentially immersing the wall-broken pollen or the filter dregs in water, alcohol and diluted acid, extracting, filtering, concentrating and mixing the concentrated extracts. The method has the advantages of low cost, comprehensive extraction of effective components contained in the pollen, extraction rate of solid matters up to about 70%, clear and transparent finished product, rich nutrition, and better maintenance of the original edible and health-care effects of the natural pollen.
Description
The invention belongs to pollen food, the drink manufacturing industry is a kind of relevant pollen broken wall and include the method that effective nutritional labeling is extracted.
Pollen is rich in multiple nutritional components and active material; And can regulate the multiple function of human body and be subjected to people's attention day by day, but pollen wall system is respectively by cellulose and pectin, outer two-layer shell was formed within lignin and pollenin constituted, high pressure resistant and the soda acid of energy, directly edible not only food flavor is not good, and is difficult to be digested and assimilated by human body.Around the problems referred to above,, make the pollen precision method that is easily absorbed and carried out extensive studies by human consumption with regard to the broken wall treatment and the extraction that includes active ingredient of pollen wall.Aspect the pollen broken wall processing, the clear 56-20823 of Japan Patent " special permission communique " " edible pollen manufacture method " has introduced the method for pollen spontaneous fermentation, it is made up of two technologies: the one, pollen water content is transferred to 14-30%, and spread on culture plate, average thickness is 2-4 centimetre; The 2nd, above-mentioned pollen after 48-72 hour, is being carried out drying at 36-38 ℃ of normal temperature bottom fermentation again below 50 ℃, promptly make powdery or granular edible pollen.But this method is when the enzyme that utilizes pollen self to contain carries out spontaneous fermentation, and the entrained assorted bacterium of pollen also will inevitably growth and breeding, consumes battalion certain in the pollen and supports And and pollute unavoidably.Aspect the broken pollen technology for extracting effective component, Chinese invention patent ublic specification of application CN86106662A " manufacture method of multiple pollen essence " has introduced the selection benzinum, edible ethanol, distilled water is solvent, at first adds a kind of solvent in pollen, after a kind of pollen essence is made in extraction, in the filter residue of gained, add another kind of solvent again, extract separation again, multiple pollen essence is made in continued operation like this, and gross production rate is 91.37% of a propollen amount.Measure greatly but this method has with an organic solvent, often, the rate of recovery is low, the cost height; Next adopts 95% alcohol in high concentration as solvent, will cause contained albuminous degeneration of pollen and enzymatic activity to descend; Be that same pollen raw material is made multiple pollen essence through the extraction of different solvents order once more, every kind of pollen essence only contains the composition that is dissolved in certain kind solvent like this, take the back just can not bring into play between each active ingredient of propollen intrinsic synergy, influenced result of use.
The objective of the invention is to overcome above-mentioned shortcoming, according to including the active material physicochemical property in the pollen, propose a kind of industrial-scale production that is suitable for, the pollen broken wall effect is better, and extracts active ingredients is comprehensive, yield height, the refining making method of the pollen that cost is low.
Manufacture method of the present invention at first adds the distilled water of pollen amount 80-300% in pollen, 35-55 ℃ is stirred down, natural enzymolysis 20-36 hour broken wall treatment, and then respectively with water, alcohol, diluted acid are solvent, carry out the extraction of following steps:
(1) add propollen amount 6-8 distilled water doubly in the pollen after broken wall treatment, 35-55 ℃ is stirred and soaked 4-8 hour, add filter aid filter filter residue (I), filtrate concentrates down at 42-50 ℃, concentrate (A);
(2) filter residue (I) is with 2-5 times of the propollen amount, and concentration is the ethanol of 20-60%, and 55-65 ℃ was soaked 3-6 hour, adds filter aid after the cooling and filters, and gets filter residue (II), and filtrate concentrates back end hydrogenation potassium oxide or NaOH saponification, gets concentrate (B);
(3) filter residue (II) is used and propollen equivalent, the following dilute hydrochloric acid solution of PH2.5, after soaking 10-30 hour under 80-90 ℃, add 4-6 times of distilled water of propollen amount, 75 ℃ were soaked 2-4 hour, cooling back end hydrogenation potassium oxide is neutralized to PH4.0, add concentrate (B) and mix, add filter aid and filter, concentrating filter liquor gets concentrate (C), add concentrate (A) again and mix, the pollen essence.
Pollen natural enzymolysis broken wall treatment of the present invention, be under the condition of liquid state and 35-55 ℃, to carry out, enzymolysis activity is good, and assorted bacterium activity is restrained, enzymolysis after most of pollen germination hole of microscopy and wall shell break, and there is not living contaminants, again through water, alcohol, three roads of diluted acid are handled, make the active ingredient of the contained different dissolubilities of pollen all obtain better extract, pollen soluble solid extraction rate reached about 70%, organic solvent (ethanol) only uses once in the refining making method of this pollen, and cost is lower, the clear wash rice of finished product is transparent, free from extraneous odour is sought rich comprehensive, abundant, kept natural pollen original edible preferably, health-care effect.
The embodiment of the invention:
The example I:
Get pollen 500 grams, 600 milliliters of adding distil waters, shape is in a liquid state after the stirring, temperature keeps 35-55 ℃, after intermittently stirring 30 hours, 2500 milliliters of adding distil waters, 35-55 ℃ is intermittently stirred immersion 4-8 hour, adding super-cell filters, get filter residue (I), transparent filtrate is concentrated into 700 milliliters in 42-50 ℃ of following vacuum, get concentrate (A), filter residue (I) adds 1000 milliliters of 60% ethanol, 55-65 ℃ is intermittently stirred immersion 3-6 hour, adding super-cell after the cooling filters, get filter residue (II) filtrate vacuum and be concentrated into 250 milliliters, add 20 milliliters of saponification of 20% potassium hydroxide again, concentrate (B), get 500 milliliters of 2% hydrochloric acid solutions again and put under 90 ℃ of liquid temperature and soaked filter residue (II) 10-30 hour, add water again and under 75 ℃ of liquid temperature, soaked 2-4 hour for 2000 milliliters, cooling back end hydrogenation potassium oxide is neutralized to PH4.0, concentrate (B) is added again and mix, add super-cell and filter, the filtrate vacuum is concentrated into 300 milliliters, be transparence concentrate (C), add concentrate (A) mixing again and promptly get smart 1000 milliliters of transparent liquid pollen.
The example II:
Get pollen 500 grams, add 25 milliliters of close or 50 gram radish Juice of honeybee, 575 milliliters in water, other method gets smart 1000 milliliters of pollen at last with the embodiment I.
Claims (2)
1, a kind of manufacture method of pollen essence, after pollen carried out broken wall treatment, add entry or organic solvent extracts, filter, concentrate, its feature adds the distilled water of the 80-300% of pollen amount earlier in pollen, 35-55 ℃ is stirred natural enzymolysis 20-36 hour down, then with water, alcohol, diluted acid are solvent, carry out the extraction of following three steps:
Add propollen amount 6-8 distilled water doubly in-the pollen after broken wall treatment, 35-55 ℃ is stirred and soaked 4-8 hour, add filter aid filter filter residue (I), filtrate concentrates down at 42-50 ℃, concentrate (A);
-filter residue (I) is with 2-5 times of the propollen amount, and concentration is the ethanol of 20-60%, and 55-65 ℃ was soaked 3-6 hour, adds filter aid after the cooling and filters, and gets filter residue (II), and filtrate concentrates back end hydrogenation potassium oxide or NaOH saponification, gets concentrate (B);
The dilute hydrochloric acid solution of-filter residue (II) below the PH2.5 with propollen equivalent, after soaking 10-30 hour under 80-90 ℃, add 4-6 times of distilled water of propollen amount, 75 ℃ were soaked 2-4 hour, cooling back end hydrogenation potassium oxide is neutralized to PH4.0, adds concentrate (B) and mixes, and adds filter aid and filters, concentrating filter liquor gets concentrate (C), adds concentrate (A) again and mixes.
2, by the described manufacture method of claim 1, its feature also can add the close or radish Juice of 5-20% honeybee in pollen broken wall is handled.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN87106139A CN1026167C (en) | 1987-09-03 | 1987-09-03 | Preparation process of the pollen essence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN87106139A CN1026167C (en) | 1987-09-03 | 1987-09-03 | Preparation process of the pollen essence |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN87106139A true CN87106139A (en) | 1988-02-10 |
| CN1026167C CN1026167C (en) | 1994-10-12 |
Family
ID=4815598
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN87106139A Expired - Fee Related CN1026167C (en) | 1987-09-03 | 1987-09-03 | Preparation process of the pollen essence |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1026167C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061973C (en) * | 1993-10-27 | 2001-02-14 | 唐孝 | Technology for extracting amino acid of animal and plant |
| CN1101148C (en) * | 2000-04-25 | 2003-02-12 | 浙江大学 | Process for breaking wall of pollen |
| CN101518303B (en) * | 2009-04-10 | 2011-10-26 | 南京师范大学 | Wet-type wall-breaking method for pollen |
| CN102242014A (en) * | 2010-05-11 | 2011-11-16 | 天津帅博科技有限公司 | Extraction technology of fresh rose liquid |
| CN102302109A (en) * | 2011-07-12 | 2012-01-04 | 新疆维吾尔自治区药物研究所 | Preparation method of safflower pollen |
| CN103211137A (en) * | 2013-01-05 | 2013-07-24 | 伊犁拉合曼蜂业科技有限责任公司 | Bionic enzymatic pollen wall-breaking method |
| CN103535835A (en) * | 2012-07-13 | 2014-01-29 | 北京市蜂业公司 | Solid beverage prepared from pollen soluble extracts |
| CN109430783A (en) * | 2018-11-12 | 2019-03-08 | 湖南德海制药有限公司 | A kind of enzyme solution of pollen |
-
1987
- 1987-09-03 CN CN87106139A patent/CN1026167C/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061973C (en) * | 1993-10-27 | 2001-02-14 | 唐孝 | Technology for extracting amino acid of animal and plant |
| CN1101148C (en) * | 2000-04-25 | 2003-02-12 | 浙江大学 | Process for breaking wall of pollen |
| CN101518303B (en) * | 2009-04-10 | 2011-10-26 | 南京师范大学 | Wet-type wall-breaking method for pollen |
| CN102242014A (en) * | 2010-05-11 | 2011-11-16 | 天津帅博科技有限公司 | Extraction technology of fresh rose liquid |
| CN102302109A (en) * | 2011-07-12 | 2012-01-04 | 新疆维吾尔自治区药物研究所 | Preparation method of safflower pollen |
| CN103535835A (en) * | 2012-07-13 | 2014-01-29 | 北京市蜂业公司 | Solid beverage prepared from pollen soluble extracts |
| CN103535835B (en) * | 2012-07-13 | 2015-09-30 | 北京市蜂业公司 | Solid beverage prepared by a kind of pollen soluble extract |
| CN103211137A (en) * | 2013-01-05 | 2013-07-24 | 伊犁拉合曼蜂业科技有限责任公司 | Bionic enzymatic pollen wall-breaking method |
| CN109430783A (en) * | 2018-11-12 | 2019-03-08 | 湖南德海制药有限公司 | A kind of enzyme solution of pollen |
| CN109430783B (en) * | 2018-11-12 | 2023-01-31 | 湖南德海制药有限公司 | Enzymolysis method of pollen |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1026167C (en) | 1994-10-12 |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| C14 | Grant of patent or utility model | ||
| C19 | Lapse of patent right due to non-payment of the annual fee |