CN113907367A - Preparation method of shaddock soluble dietary fiber - Google Patents
Preparation method of shaddock soluble dietary fiber Download PDFInfo
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- CN113907367A CN113907367A CN202111188911.8A CN202111188911A CN113907367A CN 113907367 A CN113907367 A CN 113907367A CN 202111188911 A CN202111188911 A CN 202111188911A CN 113907367 A CN113907367 A CN 113907367A
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- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 244000276331 Citrus maxima Species 0.000 title description 11
- 235000001759 Citrus maxima Nutrition 0.000 title description 11
- 240000000560 Citrus x paradisi Species 0.000 claims abstract description 53
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 36
- 108090000790 Enzymes Proteins 0.000 claims abstract description 32
- 102000004190 Enzymes Human genes 0.000 claims abstract description 32
- 229910000029 sodium carbonate Inorganic materials 0.000 claims abstract description 19
- 238000001694 spray drying Methods 0.000 claims abstract description 15
- 238000010438 heat treatment Methods 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 8
- 229940088598 enzyme Drugs 0.000 claims description 31
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 24
- 239000002893 slag Substances 0.000 claims description 24
- 101710166469 Endoglucanase Proteins 0.000 claims description 18
- 238000006243 chemical reaction Methods 0.000 claims description 16
- 239000000706 filtrate Substances 0.000 claims description 15
- 238000001914 filtration Methods 0.000 claims description 15
- 238000002156 mixing Methods 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 10
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 8
- 230000000415 inactivating effect Effects 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 5
- 239000000835 fiber Substances 0.000 claims description 5
- 230000009849 deactivation Effects 0.000 claims description 3
- 239000002440 industrial waste Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 238000009776 industrial production Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 4
- 239000002699 waste material Substances 0.000 abstract description 4
- 239000002253 acid Substances 0.000 abstract description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 abstract description 2
- 239000002904 solvent Substances 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 10
- 108010059892 Cellulase Proteins 0.000 description 5
- 229940106157 cellulase Drugs 0.000 description 5
- 230000002378 acidificating effect Effects 0.000 description 4
- 238000001035 drying Methods 0.000 description 3
- 239000012467 final product Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000002411 adverse Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 239000003925 fat Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 239000001606 7-[(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]oxy-5-hydroxy-2-(4-hydroxyphenyl)chroman-4-one Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- DFPMSGMNTNDNHN-ZPHOTFPESA-N naringin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC=2C=C3O[C@@H](CC(=O)C3=C(O)C=2)C=2C=CC(O)=CC=2)O[C@H](CO)[C@@H](O)[C@@H]1O DFPMSGMNTNDNHN-ZPHOTFPESA-N 0.000 description 1
- 229940052490 naringin Drugs 0.000 description 1
- 229930019673 naringin Natural products 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
- A23L33/22—Comminuted fibrous parts of plants, e.g. bagasse or pulp
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/40—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
- A23L3/46—Spray-drying
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to the technical field of dietary fiber processing, in particular to a preparation method of soluble grapefruit dietary fiber, which comprises the following steps: s1, pretreating raw materials; s2, performing primary enzymolysis; s3, secondary enzymolysis; s4, carrying out three times of enzymolysis and S5, carrying out spray drying, wherein waste pomelo residues generated in the industrial production of pomelo are used as raw materials, which is not only beneficial to environmental protection, but also can improve the added value of pomelo, and sodium carbonate is added in the pretreatment stage of the pomelo residues, and hydroxide generated under the heating condition by the sodium carbonate is used for removing fat and protein in the pomelo residues, and simultaneously destroying the molecular structure of insoluble dietary fiber, so that the insoluble dietary fiber can be better combined with enzyme in the subsequent enzymolysis process, thereby achieving higher yield, avoiding the addition of solvents such as strong acid, strong base, ethanol and the like, being safe and efficient, and the obtained soluble dietary fiber has better water holding capacity and expansibility, and being suitable for industrial production.
Description
Technical Field
The invention relates to the technical field of dietary fiber processing, in particular to a preparation method of soluble grapefruit dietary fiber.
Background
The pomelo is an important economic crop in China, is an important raw material for producing fruit juice and jam and extracting naringin, and has high industrial value. Along with the production, a large amount of shaddock peel residues are generated, and the shaddock peel residues contain rich dietary fibers, so that if the shaddock peel residues cannot be reasonably utilized, not only is the resource waste caused, but also the environment is adversely affected.
Dietary Fiber (DF) is a generic name for polysaccharide polymers that are not digested and absorbed by the human body, has important physiological functions, and is recognized by the nutritional community as a "seventh group of nutrients" in addition to proteins, fats, carbohydrates, vitamins, minerals, water. They can be classified into Soluble Dietary Fiber (SDF) and Insoluble Dietary Fiber (IDF) according to their solubility differences. The content of SDF is found to be an important factor influencing the physiological function of the dietary fiber. Compared with IDF, SDF is more easily fermented and metabolized by intestinal flora, and lowers blood sugar response and plasma cholesterol. The preparation of the soluble shaddock fibers can solve the problems of environmental pollution and resource waste in the industrial production process, improve the added value of products, and can also be used as an additive in the food processing process to improve the performance of food. Therefore, the development of a preparation method of the grapefruit soluble dietary fiber is of great significance.
At present, SDF is prepared by means of chemical and enzymatic methods. The chemical method mainly depends on solvents such as strong acid, strong base and the like to modify the raw materials, so that the structure of IDF is damaged and the IDF is converted into SDF. The enzymatic method mostly uses acidic cellulase as a catalyst, and the acidic cellulase is directly added into a solution for enzymolysis at one time. The acidic cellulase is a complex enzyme, mainly comprises exo-beta-glucanase, endo-beta-glucanase, beta-glucosidase and the like, can act on part of short-chain soluble fibers while hydrolyzing long-chain cellulose, and influences the yield and the quality of a final product. In addition, the enzyme preparation is added into the reaction system all at once, and along with the gradual generation of products, the enzyme also has product inhibition effect, so that the reaction is influenced, and meanwhile, partial products are further hydrolyzed by the enzyme, and finally, the quality and the yield of the product are reduced.
In conclusion, the invention solves the existing problems by designing the preparation method of the grapefruit soluble dietary fiber.
Disclosure of Invention
The invention aims to provide a preparation method of shaddock soluble dietary fiber, and aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a preparation method of soluble grapefruit dietary fibers comprises the following specific steps:
s1, pretreatment of raw materials: collecting and crushing industrial waste grapefruit slag, screening, mixing the screened grapefruit slag with a sodium carbonate solution, reacting for 1-1.5 hours in a water bath kettle at the temperature of 70-90 ℃, and cooling to room temperature;
s2, primary enzymolysis: adding a proper amount of water, adjusting the pH value to 8-9 by using citric acid/disodium hydrogen phosphate, heating a water bath to 50-55 ℃, preserving the temperature for 20min, adding alkaline cellulase for reaction, inactivating the enzyme after reacting for 2-4 h, filtering while hot, and collecting filtrate for later use;
s3, secondary enzymolysis: mixing the filter residue obtained in the step S2 with a proper amount of water, adjusting the pH to 8-9 by using citric acid/disodium hydrogen phosphate, heating to 50-55 ℃, adding alkaline cellulase again for reaction, carrying out reaction for 2-4 h again, inactivating enzyme, filtering while hot, and collecting filtrate;
s4, three times of enzymolysis: repeating S3;
s5, spray drying: and collecting the three filtrates, mixing, concentrating to 1/2 of the original volume by using a vacuum concentration tank, and spray drying to obtain a solid, namely the soluble fiber.
In a preferable scheme of the invention, the concentration of the sodium carbonate solution in the S1 is 1-3%, the addition amount of the sodium carbonate solution is 10-15 times of the mass of the grapefruit slag, the sodium carbonate solution is a mixture of sodium carbonate and water, the addition amount of the water is 10-12 times of the mass of the grapefruit slag, and the room temperature is 24-25 ℃.
In a preferable embodiment of the invention, the addition amount of the water in the S2 is 25-30 times of the mass of the grapefruit slag, the enzyme activity of the alkaline cellulase is 15000u/ml, and the addition amount of the enzyme is 0.5% of the mass of the grapefruit slag.
As a preferable scheme of the invention, the enzyme deactivation modes in S2 and S3 are both increased to 100 ℃, and the temperature is maintained for 15-20 min.
In a preferable embodiment of the invention, the addition amount of the water in the S3 is 35-45 times of the mass of the grapefruit slag, the enzyme activity of the alkaline cellulase is 15000u/ml, and the addition amount of the enzyme is 0.5% of the mass of the grapefruit slag.
As a preferable scheme of the invention, the temperature of the spray drying inlet air in the S5 is 170-190 ℃, the screen in the S1 is a 50-mesh screen, and the filtration in the S2 and the filtration in the S3 are both performed by adopting a plate-and-frame filter press.
Compared with the prior art, the invention has the beneficial effects that:
1. in the invention, waste pomelo residues generated in the industrial production of pomelo are used as raw materials, which is not only beneficial to environmental protection, but also can improve the added value of pomelo, and sodium carbonate is added in the pretreatment stage of the pomelo residues, and the hydroxide generated by the sodium carbonate under the heating condition is used for removing fat and protein in the pomelo residues, and simultaneously destroying the molecular structure of insoluble dietary fiber, so that the pomelo residues can be better combined with enzyme in the subsequent enzymolysis process, thereby avoiding the adoption of strong acid and strong alkali for raw material treatment and being beneficial to reducing chemical pollution.
2. According to the invention, alkaline cellulase is adopted for enzymolysis, the alkaline cellulase only has endo-beta-glucanase and can only act on the inside of long-chain insoluble dietary fibers, and the short-chain soluble dietary fibers have low or almost no activity, so that the problem that part of short-chain soluble dietary fibers are hydrolyzed in the enzymolysis process of the acidic cellulase can be effectively avoided, and the quality and yield of products can be improved.
3. According to the invention, enzymolysis is carried out by adding a small amount of cellulase for multiple times, so that the product inhibition effect and the phenomenon that part of products are excessively hydrolyzed caused by adding the enzyme preparation for one time can be effectively avoided, the yield of the product is favorably improved, spray drying is adopted as a drying mode of the product, the problem that other methods such as an alcohol precipitation method need to introduce organic solvents such as ethanol and the like for treatment and then drying is avoided, the production cost is reduced, and the adverse effect of the organic solvents on the environment is also reduced.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
The invention provides a technical scheme that:
a preparation method of soluble grapefruit dietary fibers comprises the following specific steps:
s1, pretreatment of raw materials: collecting and crushing industrial waste grapefruit slag, screening, mixing the screened grapefruit slag with a sodium carbonate solution, reacting for 1-1.5 hours in a water bath kettle at the temperature of 70-90 ℃, and cooling to room temperature;
s2, primary enzymolysis: adding a proper amount of water, adjusting the pH value to 8-9 by using citric acid/disodium hydrogen phosphate, heating a water bath to 50-55 ℃, preserving the temperature for 20min, adding alkaline cellulase for reaction, inactivating the enzyme after reacting for 2-4 h, filtering while hot, and collecting filtrate for later use;
s3, secondary enzymolysis: mixing the filter residue obtained in the step S2 with a proper amount of water, adjusting the pH to 8-9 by using citric acid/disodium hydrogen phosphate, heating to 50-55 ℃, adding alkaline cellulase again for reaction, carrying out reaction for 2-4 h again, inactivating enzyme, filtering while hot, and collecting filtrate;
s4, three times of enzymolysis: repeating S3;
s5, spray drying: and collecting the three filtrates, mixing, concentrating to 1/2 of the original volume by using a vacuum concentration tank, and spray drying to obtain a solid, namely the soluble fiber.
In a further preferable embodiment of the present invention, the concentration of the sodium carbonate solution in S1 is 1-3%, the addition amount is 10-15 times of the mass of the grapefruit slag, the sodium carbonate solution is a mixture of sodium carbonate and water, the addition amount of water is 10-12 times of the mass of the grapefruit slag, and the room temperature is 24-25 ℃.
In a further preferable embodiment of the invention, the addition amount of the water in the S2 is 25-30 times of the mass of the grapefruit slag, the enzyme activity of the alkaline cellulase is 15000u/ml, and the addition amount of the enzyme is 0.5% of the mass of the grapefruit slag.
As a preferable scheme of the invention, the enzyme deactivation modes in S2 and S3 are both increased to 100 ℃, and the temperature is maintained for 15-20 min.
In a further preferable embodiment of the invention, the addition amount of the water in the S3 is 35-45 times of the mass of the grapefruit slag, the enzyme activity of the alkaline cellulase is 15000u/ml, and the addition amount of the enzyme is 0.5% of the mass of the grapefruit slag.
According to a further preferable scheme of the invention, the temperature of the spray drying inlet air in S5 is 170-190 ℃, the screen in S1 is a 50-mesh screen, and the filtration in S2 and S3 are both performed by adopting a plate-and-frame filter press.
Detailed description of the preferred embodiments
Example 1
(1) Pretreatment of raw materials: crushing the collected pomelo residues, sieving the crushed pomelo residues with a 50-mesh sieve, weighing 100g of pomelo residues, adding 1g of sodium carbonate and 1000ml of water, reacting in a water bath kettle at 85 ℃ for 1.5h, taking out and cooling to room temperature;
(2) primary enzymolysis: adding 3000ml of water, adjusting the pH value to about 9 by using citric acid/disodium hydrogen phosphate, preserving the heat in a 55 ℃ water bath kettle for 20min, adding 1.5ml of alkaline cellulase, reacting for 3h, heating to 100 ℃ to inactivate the enzyme for 20min, filtering while the solution is hot, and collecting the filtrate for later use;
(3) secondary enzymolysis: collecting the filter residue in the step (2), uniformly mixing the filter residue with 4000ml of water, adjusting the pH value to about 9, adding 1.5ml of alkaline cellulase again for reaction, heating to inactivate enzyme after 3 hours, filtering when the solution is hot, and collecting the filtrate for later use;
(4) and (3) carrying out third enzymolysis: repeating the step (3);
(5) spray drying: mixing the obtained filtrates, concentrating to 1/2 of original volume, and spray drying the concentrated solution at 180 deg.C to obtain the final product;
finally, 25g of shaddock soluble dietary fiber with the content of 91.8 percent and the yield of 22.95 percent is tested, the water holding capacity of the shaddock soluble dietary fiber is 9.75g/g, and the expansibility of the shaddock soluble dietary fiber reaches 6.11 ml/g.
Example 2
(1) Pretreatment of raw materials: drying and crushing collected pomelo residues, weighing 1.5kg of pomelo residues, 150g of sodium carbonate and 15L of water, adding into a reaction kettle, heating to about 85 ℃ while stirring, maintaining the temperature for 1.5h, stopping the reaction, and cooling to room temperature;
(2) primary enzymolysis: supplementing 45L of water into the reaction kettle, adjusting the pH to about 9 by using citric acid/disodium hydrogen phosphate, heating to about 52 ℃, adding 23ml of alkaline cellulase for enzymolysis, raising the temperature to 100 ℃ after reacting for 3 hours, inactivating the enzyme for 20min, filtering by a plate-and-frame filter press while the solution is hot for solid-liquid separation, and collecting the filtrate for later use;
(3) secondary enzymolysis: adding the filter residue obtained in the step (2) into the reaction kettle again, adding 60L of water, adjusting the pH value to about 9, adding 23ml of alkaline cellulase again for enzymolysis, reacting for 3 hours, inactivating the enzyme again, filtering while hot, and collecting the filtrate for later use;
(4) and (3) carrying out third enzymolysis: repeating the step (3);
(5) spray drying: mixing the obtained filtrates, concentrating to 1/2 with original volume in a vacuum concentration tank, and spray drying at air inlet temperature of 180 deg.C with the aid of a spray dryer to obtain the final product;
finally, 375g of shaddock soluble dietary fiber is obtained, the content is 92.1 percent, the yield is 23 percent, and the water holding capacity and the expansibility are respectively 9.81g/g and 6.10 ml/g.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. The preparation method of the grapefruit soluble dietary fiber is characterized by comprising the following steps:
s1, pretreatment of raw materials: collecting and crushing industrial waste grapefruit slag, screening, mixing the screened grapefruit slag with a sodium carbonate solution, reacting for 1-1.5 hours in a water bath kettle at the temperature of 70-90 ℃, and cooling to room temperature;
s2, primary enzymolysis: adding a proper amount of water, adjusting the pH value to 8-9 by using citric acid/disodium hydrogen phosphate, heating a water bath to 50-55 ℃, preserving the temperature for 20min, adding alkaline cellulase for reaction, inactivating the enzyme after reacting for 2-4 h, filtering while hot, and collecting filtrate for later use;
s3, secondary enzymolysis: mixing the filter residue obtained in the step S2 with a proper amount of water, adjusting the pH to 8-9 by using citric acid/disodium hydrogen phosphate, heating to 50-55 ℃, adding alkaline cellulase again for reaction, carrying out reaction for 2-4 h again, inactivating enzyme, filtering while hot, and collecting filtrate;
s4, three times of enzymolysis: repeating S3;
s5, spray drying: and collecting the three filtrates, mixing, concentrating to 1/2 of the original volume by using a vacuum concentration tank, and spray drying to obtain a solid, namely the soluble fiber.
2. The preparation method of the grapefruit soluble dietary fiber according to claim 1, wherein the concentration of the sodium carbonate solution in S1 is 1-3%, the addition amount is 10-15 times of the mass of grapefruit slag, the sodium carbonate solution is a mixture of sodium carbonate and water, the addition amount of water is 10-12 times of the mass of grapefruit slag, and the room temperature is 24-25 ℃.
3. The preparation method of the grapefruit soluble dietary fiber according to claim 1, wherein the addition amount of water in S2 is 25-30 times of the mass of grapefruit slag, the enzyme activity of the alkaline cellulase is 15000u/ml, and the addition amount of the enzyme is 0.5% of the mass of grapefruit slag.
4. The method for preparing the grapefruit soluble dietary fiber according to claim 1, wherein the enzyme deactivation modes in the S2 and S3 are both heating to 100 ℃, and the temperature is maintained for 15-20 min.
5. The preparation method of the grapefruit soluble dietary fiber according to claim 1, wherein the addition amount of water in S3 is 35-45 times of the mass of grapefruit slag, the enzyme activity of the alkaline cellulase is 15000u/ml, and the addition amount of the enzyme is 0.5% of the mass of grapefruit slag.
6. The preparation method of the grapefruit soluble dietary fiber according to claim 1, wherein the spray drying inlet air temperature in the S5 is 170-190 ℃, the screen in the S1 is a 50-mesh screen, and the filtration in the S2 and the filtration in the S3 are both performed by a plate-and-frame filter press.
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|---|---|---|---|---|
| CN115595286A (en) * | 2022-11-07 | 2023-01-13 | 东莞石龙津威饮料食品有限公司（Cn） | Lactobacillus plantarum microbial inoculum and preparation method and application thereof |
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