CN1026167C

CN1026167C - Preparation process of the pollen essence

Info

Publication number: CN1026167C
Application number: CN87106139A
Authority: CN
Inventors: 陈传盈; 许尧兴; 袁亚
Original assignee: Zhejiang Academy of Agricultural Sciences
Current assignee: Zhejiang Academy of Agricultural Sciences
Priority date: 1987-09-03
Filing date: 1987-09-03
Publication date: 1994-10-12
Anticipated expiration: 2002-09-03
Also published as: CN87106139A

Abstract

The present invention relates to the preparation process of pollen essence. Pollen is first mixed with water into a liquid state, and the wall shell of pollen is split under the action of natural enzymolysis at a temperature of 35 to 55 DEG C. Then the wall-split pollen or the filtered dregs of the preceding step is respectively soaked by water, alcohol and dilute acid. After extraction, filtration and concentration, pollen essence is finally obtained by mixing the different concentrated liquids extracted by different solvents. The process has low cost, the effective components of pollen are completely extracted, and the extraction rate is about 70%. The finished product is clear and transparent in appearance and rich in nutrition, and the preparation process fairly keeps the edible and health-care effect of natural pollen.

Description

Preparation process of the pollen essence

The invention belongs to pollen food, the drink manufacturing industry is a kind of relevant pollen broken wall and include the method that effective nutritional labeling is extracted.

Pollen is rich in multiple nutritional components and active material, and can regulate the multiple function of human body and be subjected to people day by day and pay attention to, but pollen wall system is respectively by cellulose and pectin, outer two-layer shell was formed within lignin and pollenin constituted, high pressure resistant and the soda acid of energy, directly edible not only food flavor is not good, and is difficult to be digested and assimilated by human body.Around the problems referred to above,, make the pollen precision method that is easily absorbed and carried out extensive studies by human consumption with regard to the broken wall treatment and the extraction that includes active ingredient of pollen wall.Aspect the pollen broken wall processing, the clear 56-20823 of Japan Patent " special permission communique " " edible pollen manufacture method " has introduced the method for pollen spontaneous fermentation, it is made up of two technologies: the one, pollen water content is transferred to 14-30%, and spread on culture plate, average thickness is 2-4 centimetre; The 2nd, above-mentioned pollen after 48-72 hour, is being carried out drying at 36-38 ℃ of normal temperature bottom fermentation again below 50 ℃, promptly make powdery or granular edible pollen.But this method is when the enzyme that utilizes pollen self to contain carries out spontaneous fermentation, and the entrained assorted bacterium of pollen also will inevitably growth and breeding, consumes nutrition certain in the pollen and pollutes unavoidably.Aspect the broken pollen technology for extracting effective component, Chinese invention patent ublic specification of application CN86106662A " manufacture method of multiple pollen essence " has introduced selection benzinum, edible ethanol, distilled water is solvent, at first in pollen, add a kind of solvent, after a kind of pollen essence is made in extraction, in the filter residue of gained, add another kind of solvent again, extract separation again, multiple pollen essence is made in continued operation like this, and gross production rate is 91.37% of a propollen amount.Measure greatly but this method has with an organic solvent, often, the rate of recovery is low, the cost height; Next adopts 95% alcohol in high concentration as solvent, will cause contained albuminous degeneration of pollen and enzymatic activity to descend; To be same pollen raw material once more through the different solvents order extract makes multiple pollen essence, so every kind of pollen essence only contain the composition that is dissolved in certain kind solvent, take the back just can not bring into play between each active ingredient of propollen intrinsic synergy, influenced result of use.

The objective of the invention is to overcome above-mentioned shortcoming, according to including the active material physicochemical property in the pollen, propose a kind of industrial-scale production that is suitable for, the pollen broken wall effect is better, and extracts active ingredients is comprehensive, yield height, the refining making method of the pollen that cost is low.

Manufacture method of the present invention at first adds the distilled water of pollen amount 80-300% in pollen, 35-55 ℃ is stirred down, natural enzymolysis 20-36 hour broken wall treatment, and then respectively with water, alcohol, diluted acid are solvent, carry out the extraction of following steps:

(1) add propollen amount 6-8 distilled water doubly in the pollen after broken wall treatment, 35-55 ℃ is stirred and soaked 4-8 hour, add filter aid filter filter residue (I), filtrate concentrates down at 42-50 ℃, concentrate (A);

(2) filter residue (I) is with 2-5 times of the propollen amount, and concentration is the ethanol of 20-60%, and 55-65 ℃ was soaked 3-6 hour, adds filter aid after the cooling and filters, and gets filter residue (II), and filtrate concentrates back end hydrogenation potassium oxide or NaOH saponification, gets concentrate (B);

(3) filter residue (II) is used and propollen equivalent, the following dilute hydrochloric acid solution of PH2.5, after soaking 10-30 hour under 80-90 ℃, add 4-6 times of distilled water of propollen amount, 75 ℃ were soaked 2-4 hour, cooling back end hydrogenation potassium oxide is neutralized to PH4.0, add concentrate (B) and mix, add filter aid and filter, concentrating filter liquor gets concentrate (C), add concentrate (A) again and mix, the pollen essence.

Pollen natural enzymolysis broken wall treatment of the present invention, be under the condition of liquid state and 35-55 ℃, to carry out, enzymolysis activity is good, and assorted bacterium activity is restrained, and enzymolysis and does not have living contaminants after most of pollen germination hole of microscopy and wall shell break, again through water, alcohol, three roads of diluted acid are handled, and make the active ingredient of the contained different dissolubilities of pollen all obtain better extract, pollen soluble solid extraction rate reached about 70%, organic solvent (ethanol) only uses once in the refining making method of this pollen, cost is lower, and finished product is Clear ﹠ Transparent, free from extraneous odour, comprehensive nutrition, abundant, kept natural pollen original edible preferably, health-care effect.

The embodiment of the invention:

The example I:

Get pollen 500 grams, 600 milliliters of adding distil waters, shape is in a liquid state after the stirring, temperature keeps 35-55 ℃, after intermittently stirring 30 hours, 2500 milliliters of adding distil waters, 35-55 ℃ is intermittently stirred immersion 4-8 hour, adding super-cell filters, get filter residue (I), transparent filtrate is concentrated into 700 milliliters in 42-50 ℃ of following vacuum, get concentrate (A), filter residue (I) adds 1000 milliliters of 60% ethanol, 55-65 ℃ is intermittently stirred immersion 3-6 hour, adding super-cell after the cooling filters, get filter residue (II) filtrate vacuum and be concentrated into 250 milliliters, add 20 milliliters of saponification of 20% potassium hydroxide again, concentrate (B), get 500 milliliters of 2% hydrochloric acid solutions again and put under 90 ℃ of liquid temperature and soaked filter residue (II) 10-30 hour, add water again and under 75 ℃ of liquid temperature, soaked 2-4 hour for 2000 milliliters, cooling back end hydrogenation potassium oxide is neutralized to PH4.0, concentrate (B) is added again and mix, add super-cell and filter, the filtrate vacuum is concentrated into 300 milliliters, be transparence concentrate (C), add concentrate (A) mixing again and promptly get smart 1000 milliliters of transparent liquid pollen.

The example II:

Get pollen 500 grams, add 25 milliliters of close or 50 gram radish Juice of honeybee, 575 milliliters in water, other method gets smart 1000 milliliters of pollen at last with the embodiment I.

Claims

1, a kind of manufacture method of pollen essence, after pollen carried out broken wall treatment, add entry or organic solvent extracts, filter, concentrate, its feature adds the distilled water of the 80-300% of pollen amount earlier in pollen, 35-55 ℃ is stirred natural enzymolysis 20-36 hour down, then with water, alcohol, diluted acid are solvent, carry out the extraction of following three steps;

Add propollen amount 6-8 distilled water doubly in-the pollen after broken wall treatment, 35-55 ℃ is stirred and soaked 4-8 hour, add filter aid filter filter residue (I), filtrate concentrates down at 42-50 ℃, concentrate (A);

-filter residue (I) is with 2-5 times of the propollen amount, and concentration is the ethanol of 20-60%, and 55-65 ℃ was soaked 3-6 hour, adds filter aid after the cooling and filters, and gets filter residue (II), and filtrate concentrates back end hydrogenation potassium oxide or NaOH saponification, gets concentrate (B);

The dilute hydrochloric acid solution of-filter residue (II) below the pH2.5 with propollen equivalent, after soaking 10-30 hour under 80-90 ℃, adding 4-6 times of distilled water of propollen amount soaked 2-4 hour for 75 ℃, cooling back end hydrogenation potassium oxide is neutralized to pH4.0, adding concentrate (B) mixes, add filter aid and filter, concentrating filter liquor gets concentrate (C), adds concentrate (A) again and mixes.

2, by the described manufacture method of claim 1, its feature also can add 5-20% honey or radish Juice in pollen broken wall is handled.