CN1101148C - Process for breaking wall of pollen - Google Patents

Process for breaking wall of pollen Download PDF

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Publication number
CN1101148C
CN1101148C CN00105084A CN00105084A CN1101148C CN 1101148 C CN1101148 C CN 1101148C CN 00105084 A CN00105084 A CN 00105084A CN 00105084 A CN00105084 A CN 00105084A CN 1101148 C CN1101148 C CN 1101148C
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China
Prior art keywords
pollen
radiation
present
wall
food
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Expired - Fee Related
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CN00105084A
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Chinese (zh)
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CN1273784A (en
Inventor
苏松坤
陈盛禄
林雪珍
傅凌
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Zhejiang University ZJU
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Zhejiang University ZJU
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Priority to CN00105084A priority Critical patent/CN1101148C/en
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  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Jellies, Jams, And Syrups (AREA)

Abstract

The present invention discloses a pollen wall breaking method which comprises: fresh pollen or dried pollen is put in a container made from a radioprotective food packaging material. The container is put under a radiation point used for food radiation at a normal temperature and at normal pressure. The radiation dosage is from 1KG y to 11KG y, and the radiation time is 24 hours. The present invention has high wall breaking ratio, and has sterilization effect. Because the integral pollen grains are not destroyed, the present invention is favorable to nutrient component conservation. Furthermore, the present invention has simple and convenient operation, energy conservation and no chemical waste which pollute the environment, and is especially suitable for mass industrial production.

Description

Process for breaking wall of pollen
The present invention relates to a kind of wall-breaking method of pollen.
Pollen is the male gametophyte of plant generative propagation.The nutritional labeling of pollen is very abundant, contains protein, amino acid, carbohydrate, lipid, vitamin, nucleic acid, mineral matter, enzyme and flavone compound etc.Pollen has effects such as resistance prostatitis, anti-ageing, anti-anoxic, reducing blood lipid, is widely used in aspects such as food, beverage, cosmetics, medicine, feed.But pollen has a hard pollen wall, and the main component of pollen wall is sporopollenin, cellulose, carotenoid etc., and acidproof anticorrosive, the pollen main nutrient composition is present in the inclusion of pollen wall inside.Pollen has only the nutritional labeling of its inclusion behind broken wall to be digested and assimilated by body.
At present, the wall-breaking method of pollen mainly contains following several:
1) difference Poluo wall method: utilize the principle of expanding with heat and contract with cold, pollen drops into rapidly after cryogenic freezing in the hot water " bursting ", and this method is destroyed bigger to the nutritional labeling of pollen, and complicated operation, and the pollen behind the broken wall also needs dry if will make pulvis.
2) colloid mill broken wall method: be to place-15~-25 ℃ to descend freezing 15 hours mixed pollen, pollen after freezing put in the water-bath melt, again by the colloid mill broken wall, this method sporoderm-broken rate is lower, pollen needs could handle after soaking into colloid, complicated operation, and the pollen behind the broken wall also needs dry if will make pulvis.
3) comminution by gas stream: be to utilize the compressed air pump air to produce high pressure draught, spray into pulverizing chamber with velocity of sound,, reach the purpose of pulverizing pollen at low temperatures by collision through nozzle.This method operation is complicated, and the pollen grain inclusion behind the broken wall is exposed in the external environment fully, is easy to generate oxidation deterioration.
4) fermentation enzymolysis broken wall method: be a kind of application method more widely, the enzyme that utilizes the abundant microorganism of pollen self or produce by microbe inoculation, make the fovilla degraded, osmotic pressure raises and makes pollen broken wall, required time of this method broken wall is long, and is easily influenced product quality by living contaminants in the sweat.
The objective of the invention is to propose to adopt radiation pollen grain integral body not to be gone to pot and rapid broken wall, and the high a kind of process for breaking wall of pollen of broken wall efficient.
In order to achieve the above object, the technical solution used in the present invention is: the pollen of fresh or drying is packed in the container that radiation-resistant packaging material for food (as glass, polyethylene) makes, under normal temperature, normal pressure, be placed on the radiation source that can be used for food irradiation, as 60C OOr 137C SDown, carry out radiation treatment, dose of radiation is 1KGy~11KGy (kilogray), is preferably 5KGy~10KGy, and the radiation treatment time is 24 hours.Preferably use vacuum-packed technique,, can adopt the packing specification of 100g, 1kg, 20kg, 50kg to reduce radiation and oxidation when storing.
The present invention compared with prior art, the useful effect that has is:
1, adopt radiation that the interior macromolecular substances (as protein, DNA etc.) of pollen cell is degraded, secondary structure is destroyed, and osmotic pressure raises in the cell; Radiation improves the cytoplasm membrane permeability, and inclusion collapses expansion from pollen germination pore easily, the effect that reaches not only broken wall but also sterilize.
2,, help nutritional labeling and preserve because pollen grain integral body does not have destroyedly, and inclusion still is present in the pollen wall under drying condition.
3, easy and simple to handle, the efficient height, energy savings does not produce the refuse of contaminated environment, is highly suitable for industrialized mass.
Be embodiments of the invention below:
Embodiment 1: take by weighing dry zasiokaurin, chrysanthemum powder, sunflower powder 10g respectively, in the vial of packing into, be placed on 60C OUnder the radiation source, respectively through 3KGy, 5KGy, 7KGy, 9KGy, 11KGy radiation treatment, the radiation treatment time is 24 hours.Get each 1g of pollen sample of radiation treatment and control group, add the 9ml distilled water diluting, observe, count, calculate sporoderm-broken rate and be listed as follows at the OLYMPUS microscopically:
The table 1 average sporoderm-broken rates of different pollen kinds (%)
The pollen kind Control group Radiation treatment dosage (KGy)
3 5 7 9
Corn 9.01 16.69 18.60 21.53 36.30
Chrysanthemum 23.7 41.2 51.1 49.7 53.7
Sunflower 19.2 41.1 45.2 51.3 52.1
Control group in table 2 his-and-hers watches 1 and result of the test are carried out variance analysis
Kind Source of variation df SS MS F Conspicuousness
Corn Between processing 5 0.785 0.157 78.2 **
In the processing 54 0.097 0.002
Chrysanthemum Between processing 5 0.89 0.178 59.33 **
In the processing 54 0.184 0.003
Sunflower Between processing 5 0.79 0.158 31.6 **
In the processing 54 0.269 0.005
Annotate: df: the free degree
SS: sum of sguares of deviation from mean
MS: sample mean variance
F: all square between processing to error average variance ratio
*: expression difference is extremely remarkable
Through variance analysis, find that radiation treatment is remarkable to the sporoderm-broken rate effect that improves pollen such as corn, chrysanthemum, sunflower.
Embodiment 2: take by weighing dry tealeaves pollen 10g, its wall-breaking method is identical with embodiment 1, and its sporoderm-broken rate is listed as follows:
The average sporoderm-broken rate of the different place of production of table 3 camellia powder (%)
The place of production Control group Radiation treatment dosage (KGy)
3 5 7 9 11
Linan, Zhejiang 20.5 85.7 95.9 98.2 95.8 98.5
Yixing, Jiangsu 9.0 88.11 95.2 98.3 98.1 98.1
Chunan, Zhejiang 38.4 82.6 86.6 94.6 97.2 96.9
Anhui 39.8 93.6 95.1 95.0 97.1 95.7
The Tonglu, Zhejiang 57.7 80.6 90.4 94.4 94.7 96.9
Table 4 his-and-hers watches 3 control groups and test group result carry out variance analysis
The place of production Source of variation df SS MS F Conspicuousness
Linan, Zhejiang Between processing 5 2.355 0.471 471 **
In the processing 24 0.019 0.001
Yixing, Jiangsu Between processing 5 3.160 0.632 632 **
In the processing 24 0.028 0.001
Chunan, Zhejiang Between processing 5 1.226 0.253 84.33 **
In the processing 24 0.074 0.003
Anhui Between processing 5 1.287 0.037 18.5 **
In the processing 24 0.059 0.002
The Tonglu, Zhejiang Between processing 5 0.556 0.111 37 **
In the processing 24 0.075 0.003
Through variance analysis, find that radiation treatment is remarkable to the sporoderm-broken rate effect that improves the camellia powder.
The expert group of coordination committee mechanism of the World Health Organization reaches a conclusion recently, can not bring any toxicologic threat to the food of the dose of radiation radiation of 10KGy, and the food of handling with this dosage no longer needs any toxicologic evaluation.

Claims (3)

1. process for breaking wall of pollen, it is characterized in that: the pollen of fresh or drying is packed in the container that radiation-resistant packaging material for food makes, under normal temperature, normal pressure, be placed under the radiation source that is used for food irradiation, carry out radiation treatment, dose of radiation is 1KGy~11KGy, and the radiation treatment time is 24 hours.
2. process for breaking wall of pollen according to claim 1 is characterized in that: dose of radiation is 5KGy~10KGy.
3. process for breaking wall of pollen according to claim 1 and 2 is characterized in that: the radiation source that is used for food irradiation is 60C OOr 137C S
CN00105084A 2000-04-25 2000-04-25 Process for breaking wall of pollen Expired - Fee Related CN1101148C (en)

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CN1101148C true CN1101148C (en) 2003-02-12

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103125800A (en) * 2013-03-11 2013-06-05 中国林业科学研究院亚热带林业研究所 Processing technology of pollen pini for preventing and controlling Indian meal moth damage
CN112121965B (en) * 2020-09-07 2021-12-10 宁燕平 Laser light energy-based spore physical wall breaking device and method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN87106139A (en) * 1987-09-03 1988-02-10 浙江省农业科学院 Method for producing pollen essence

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN87106139A (en) * 1987-09-03 1988-02-10 浙江省农业科学院 Method for producing pollen essence

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
《河南预防医学杂志》99年第2期 1994-04-01 王培仁等,"食品辐射技术的研究与应用" *
《河南预防医学杂志》99年第2期 1994-04-01 王培仁等,"食品辐射技术的研究与应用";《食品科技》94年第1期 1994-02-01 屈福才"玉米花粉的开发与利用" *
《食品科技》94年第1期 1994-02-01 屈福才"玉米花粉的开发与利用" *

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