CN112121965B - Laser light energy-based spore physical wall breaking device and method - Google Patents

Laser light energy-based spore physical wall breaking device and method Download PDF

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CN112121965B
CN112121965B CN202010930957.1A CN202010930957A CN112121965B CN 112121965 B CN112121965 B CN 112121965B CN 202010930957 A CN202010930957 A CN 202010930957A CN 112121965 B CN112121965 B CN 112121965B
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light energy
laser
spore
energy receiver
spores
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CN112121965A (en
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宁燕平
刘东波
马金亮
王浟
孙宁亚
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B02CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02CCRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C19/00Other disintegrating devices or methods
    • B02C19/18Use of auxiliary physical effects, e.g. ultrasonics, irradiation, for disintegrating
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B02CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02CCRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C23/00Auxiliary methods or auxiliary devices or accessories specially adapted for crushing or disintegrating not provided for in preceding groups or not specially adapted to apparatus covered by a single preceding group

Abstract

The invention discloses a laser light energy-based spore physical wall breaking device and a method, belonging to the technical field of spore wall breaking, wherein the spore physical wall breaking device comprises a mixing barrel, a pipeline pump, a light energy receiver and a laser; the mixing barrel, the pipeline pump and the light energy receiver are sequentially connected through pipelines, and a spore feeding hopper and a pure water inlet pipe are arranged on the mixing barrel; the pipeline pump is used for conveying the mixed liquid in the mixing barrel to the light energy receiver, and the laser is used for carrying out laser irradiation on the mixed liquid in the light energy receiver. The physical spore wall breaking method comprises adding pure water and spores into a mixing barrel to obtain mixed solution; conveying the mixed solution to a light energy receiver; carrying out laser irradiation on the side part of the light energy receiver by a laser; the spores in the mixed solution are irradiated by a light energy receptor to break the wall and then are conveyed into a finished product barrel. The invention utilizes laser light energy to physically break walls of medicinal plant spores and pollen spores without oxidation and any additive, and has high wall breaking rate.

Description

Laser light energy-based spore physical wall breaking device and method
Technical Field
The invention relates to the technical field of spore wall breaking, in particular to a device and a method for physically breaking walls of spores based on laser light energy.
Background
After the wall of the medicinal source plant spore is broken, the medicinal effect can be completely released, and the treatment effect is substantially changed due to the change of the medicinal property. After the spore is broken, water and oil in the spore are migrated, so that the surface of the spore presents a semi-moist state, stable particle groups can be formed between the microparticles, and each microparticle group contains the traditional Chinese medicine components in the same proportion. The physical structure of the corpuscle group is different with different combinations and different interactions of HLB (hydrophile-lipophile balance), ductility, fragmenting property, specific gravity and the like of each component in the composition. The structure is beneficial to the absorption and utilization of the traditional Chinese medicine by the human body. The lipid solubility of the traditional Chinese medicine is enhanced after the spore wall is broken, the traditional Chinese medicine can easily penetrate through a lipid membrane, the blood concentration in a human body is rapidly improved, and the purpose of treatment is achieved.
Pollen spores are not only the source of plant life but also really called "micro-nutrition treasury". For example, the content of protein (amino acid) in every 100 g of pollen pini spores is up to dozens of milligrams, and 22 amino acids are all in a free state and are easily absorbed by human bodies. Most pollen spores contain dietary fiber and fat, and are rich in vitamin B group and 14 vitamins such as vitamin A, vitamin D, vitamin E, vitamin C, vitamin K and the like. Several vitamins in pollen spore are proved to delay the aging process of human body cells, prolong life and beautify face. The pollen spore also contains thirty-more mineral trace elements such as iron, zinc, calcium, magnesium, potassium, selenium and the like, 104 enzymes and coenzyme, nucleic acid, lecithin, brass substances and monounsaturated polyunsaturated fatty acids, and several unsaturated fatty acids in the pollen are essential fatty acids which cannot be synthesized by a human body, so that the pollen spore can be said to be a totipotent nutritional food and a health-care product.
Pollen spores used as skin care products must be subjected to wall breaking treatment in advance, and because the moisture content of the human skin surface is low and the enzyme and acid environment are lacked, the nutrient components of the pollen spores cannot be naturally released from germination holes or germination ditches of the pollen spores. Only when the pollen spore wall is broken in advance to expose its content can the nutrients in it be directly contacted with skin and absorbed by skin.
Since the outer walls of the herb-derived plant spores and the pollen spores are composed of sporopouenin, callose, chitin, insoluble cellulose, pectin and other substances, the pollen spores are hard and have strong chemical corrosion resistance, and the tough walls of the outer layers of the spores cannot be digested by human digestive juice, so that various wall breaking techniques and methods are continuously sought. To date, spore wall breaking methods are mainly classified into the following types:
the chemical method comprises the following steps: by adopting chemical methods such as solvent soaking, acid degradation, alkali degradation and the like, the effective components of spore inclusion are often denatured because the solvent used for soaking is a chemical substance, and the harmful residues are high and difficult to remove. At present, the chemical method is eliminated.
Mechanical method: the aim of spore wall breaking is achieved through the actions of high-speed collision, vibration, grinding, shearing force and the like generated by mechanical motion, and the method has simple equipment but low wall breaking efficiency. And the problem of heavy metal pollution exists, because the wall breaking is carried out at the high temperature of mechanical movement, the phenomenon of scorching of spores generally occurs, and because the wall breaking is carried out under the natural environment, the phenomenon that the content of the spores is oxidized occurs. In addition, the wall shell after wall breaking and the spore inclusion are mixed together, thereby reducing the effective utilization rate of the spore inclusion. Although there are many problems in mechanically breaking spore wall, the so-called mechanically breaking spore powder on the market is mostly produced in small workshops due to low industry threshold, low technology content and low investment.
The biological enzyme method comprises the following steps: generally, cellulase, hemicellulase, protease, pectinase, lysozyme, helicase, chitinase and the like are used for degrading the spore wall to achieve the aim of breaking the wall. The main treatment process is as follows: the spores are soaked in a solution containing one or more of the enzymes for a period of time (usually several days). Although the biological enzyme method can degrade the outer wall of the spore (break the wall), the method cannot be adopted for breaking the wall of the spore of the medicinal plant because the enzyme in the spore content is difficult to remove.
Physical method: the main stream of physical wall breaking in the industry is a low-temperature freezing wall breaking method; ultrasonic wall breaking method; wind tunnel (high pressure) supersonic airflow wall breaking method; ultra-low temperature air flow impact wall breaking method and the like. The physical wall breaking equipment has large one-time investment, complex equipment and high production cost, and is difficult to be used for large-scale production. And the wall-breaking rate is difficult to control, and the nutrient loss of the spore content after wall breaking is very large. At present, various spore products broken by physical methods are difficult to become the mainstream of spore wall breaking.
The synthesis method comprises the following steps: i.e. combining the effects of the above-mentioned methods, and simultaneously or successively using several of them. The main reasons for the use of the comprehensive method are that the size of the spores of medicinal plants and pollen spores is very small (micron size), the outer walls of the spores are very tough, and the spores have at least one inner wall, and one method is difficult to completely destroy the whole spore wall.
It should be noted that: the spore of the medicinal plant spore and the spore of the pollen have to be broken under the physical conditions of no oxidation and no additive, and the wall is sealed and stored at low temperature after being broken. Because the spore content after wall breaking is exposed outside, the spore is easily polluted by bacteria falling and oxidized and deteriorated under the airtight environment and normal temperature.
In view of this, the present invention provides a device and a method for physically breaking spore walls based on laser light energy.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a device and a method for physically breaking the spore wall based on laser light energy.
In order to achieve the purpose, the invention provides the following technical scheme:
a laser light energy based spore physical wall breaking device comprises a mixing barrel, a pipeline pump, a light energy receiver and a laser;
the mixing barrel, the pipeline pump and the light energy receiver are sequentially connected through pipelines, and a spore feed hopper and a pure water inlet pipe are arranged on the mixing barrel so as to add spores and pure water into the mixing barrel and mix the spores and the pure water through the mixing barrel to form mixed liquid;
the pipeline pump is used for conveying the mixed liquid in the mixing barrel to the light energy receiver, and the laser is used for carrying out laser irradiation on the mixed liquid in the light energy receiver.
More preferably: the device also comprises a flow controller, a water pump and a finished product barrel;
the flow controller comprises a first flow controller and a second flow controller, and the mixing barrel, the pipeline pump, the first flow controller, the light energy receiver, the second flow controller, the water pump and the finished product barrel are sequentially connected through pipelines.
More preferably: the mixing barrel, the pipeline pump, the first flow controller and the light energy receiver are connected in sequence through a food-grade plastic conduit.
More preferably: the light energy receiver, the second flow controller, the water suction pump and the finished product barrel are connected in sequence through a medical grade plastic conduit.
More preferably: the mixing device is characterized by further comprising an electromagnetic valve, wherein the electromagnetic valve comprises a first electromagnetic valve and a second electromagnetic valve, the first electromagnetic valve is installed between the mixing barrel and the pipeline pump, and the second electromagnetic valve is installed between the water suction pump and the finished product barrel;
the first electromagnetic valve and the second electromagnetic valve are connected through a pipeline, so that the mixed liquid is sent back to the light energy receiver again.
More preferably: the light energy receiver is provided with a laser light energy receiving area, the laser light energy receiving area is made of transparent glass, and the light energy receiver is thin and flat.
More preferably: the food-grade plastic conduit is of a round pipe shape, and one end of the food-grade plastic conduit close to the light energy receiver is of a flat shape; the medical grade plastic conduit is a round pipe type, and one end of the medical grade plastic conduit close to the light energy receiver is flat.
More preferably: the spore is medicinal source plant spore or pollen spore.
More preferably: the laser is a nanosecond laser or a picosecond laser; the laser comprises one or more of a titanium gem laser, an ultraviolet-visible-near infrared band laser, an excimer laser and an ytterbium-doped fiber laser.
A laser energy based spore physical wall breaking method comprises the following steps:
s1, adding pure water and drug source plant spores or pollen spores needing wall breaking into a food-grade stainless steel mixing barrel with a stirring device, and uniformly stirring to obtain a mixed solution;
s2, conveying the mixed solution in the mixing barrel to a light energy receiver through an electrodeless direct current voltage speed-regulating micro pipeline pump, wherein the pressure and the flow speed of the mixed solution in a pipeline are controlled by a flow controller;
s3, performing laser irradiation on the mixed solution by one or more lasers at the side part of the light energy receiver to break and crush the wall of the medicinal plant spore or pollen spore needing to be broken;
s4, after the spores in the mixed solution are irradiated by the light energy receptor to break the walls, the mixed solution is conveyed to a medical-grade closed plastic finished product barrel by the water pump through the medical-grade plastic guide pipe, and then the mixed solution is subpackaged into small sealed barrels to be refrigerated and transported.
In conclusion, the invention has the following beneficial effects:
1. is suitable for industrial production. Each production line (wall breaking production line) is an independent subsystem, and production enterprises can set up N production lines according to market needs.
2. The automation degree is high. Each production line (broken wall production line) can all realize unmanned on duty (the on-the-spot staff that also can not leave of laser instrument during operation). Meanwhile, the method is conveniently expanded to carry out unified automatic control on N production lines.
3. The aim of no oxidation and no additive for breaking wall is really realized. When the spore in the mixed solution is irradiated by light in the light energy receptor to break the wall, the spore is conveyed to the closed barrel until the whole process of subpackaging, and the spore is carried out in an anaerobic environment.
4. The investment is relatively small. The main investment of each production line is a laser, the investment of other auxiliary equipment is small (a miniature direct-current voltage speed-regulating pump, a pipeline, pipeline accessories such as an electromagnetic valve and the like, a light energy receiver and the like), the investment can be basically ignored, and with the common use of the lasers, the price quotation of a single laser is reduced to be within 10 million famous currencies.
5. The phenomenon of nutrient loss of spore content hardly exists. The product from the production line is the original liquid of the wall-broken spore provided for downstream enterprises, and the downstream enterprises can carry out the post-treatment of the original liquid according to the requirements.
6. Has certain bactericidal effect. The invention has no phenomena of heavy metal pollution, coking pollution and nutrient loss of the content, but has the function of killing most of bacteria because of the laser light energy.
Therefore, the invention is a brand-new process flow with brand-new concept and without oxidation and any additive for physically breaking the walls of the medicinal plant spores and the pollen spores by using laser light energy. The method can not only obtain high wall-breaking rate, but also solve the problem that spore inclusion is easy to be oxidized or volatilized after wall breaking. After the wall of the medicinal plant spore and pollen spore is broken, the extracted inclusion can be widely applied to the production of solid preparations such as granules, capsules, tablets, films and the like, and can even be prepared into traditional Chinese medicine injections, and simultaneously, can also be widely applied to the health food industry and the beauty and skin care industry.
Drawings
FIG. 1 is a process flow diagram of example 1, which is mainly used to embody a method and an apparatus for physically breaking walls of spores;
FIG. 2 is a process flow diagram of example 2, which is mainly used to embody a method and an apparatus for physically breaking walls of spores;
FIG. 3 is a schematic structural diagram of an embodiment, which is mainly used for embodying the structure of the light energy receiver;
FIG. 4 is a schematic structural diagram of an embodiment, which is mainly used for embodying the structure of a light energy receiver provided with a plurality of laser light energy receiving areas;
FIG. 5 is a schematic structural diagram of an embodiment, which is mainly used for showing the connection mode of adjacent light energy receivers;
FIG. 6 is a microscopic view of pollen Pini spore, which is mainly used for showing the cell structure of pollen Pini spore before wall breaking;
FIG. 7 is a microscopic view of pollen Pini spore, which is mainly used for showing the cell structure of pollen Pini spore after wall breaking;
FIG. 8 is a microscopic view of a pollen spore of rape, which is mainly used for showing the cell structure of the pollen spore of rape before wall breaking;
FIG. 9 is a microscopic view of a pollen spore of rape, which is mainly used for showing the cell structure of the pollen spore of rape after wall breaking.
In the figure, 1, a mixing barrel; 2. a pipeline pump; 31. a first flow controller; 32. a second flow controller; 4. a light energy receptor; 41. a light energy receptor body; 42. a laser light energy receiving area; 5. a water pump; 6. a finished product barrel; 7. a small sealed barrel; 8. a food grade plastic conduit; 9. a medical grade plastic catheter; 10. a spore feed hopper; 11. a pure water inlet pipe; 12. a first solenoid valve; 13. a second solenoid valve.
In fig. 1-2, the arrows represent the laser beam irradiation directions.
Detailed Description
The invention is described in detail below with reference to the figures and examples.
Example 1: a laser light energy based spore physical wall breaking device and a method are disclosed, as shown in figures 1, 3, 4 and 5, the spore physical wall breaking device comprises a mixing barrel 1, a pipeline pump 2, a light energy receiver 4, a laser, a flow controller, a water pump 5, a finished product barrel 6 and a small sealing barrel 7. The flow controller comprises a first flow controller 31 and a second flow controller 32, and the mixing barrel 1, the pipeline pump 2, the first flow controller 31, the light energy receiver 4, the second flow controller 32, the water pump 5 and the finished product barrel 6 are sequentially connected through pipelines. The top of the mixing barrel 1 is provided with a spore feed hopper 10 and a pure water inlet pipe 11 to add spores and pure water into the mixing barrel 1 and mix them through the mixing barrel 1 to form a mixed solution. In order to mix the spores and pure water uniformly, the mixing barrel 1 is preferably provided with a magnetic stirring device, and the stirring form of the stirring device can be paddle type, frame type or other types.
In this embodiment, the spores are medicinal plant spores or pollen spores.
The mixing barrel 1 is a food-grade stainless steel mixing barrel, and required pure water and spores are added according to a planned proportion and are properly stirred and mixed. The proportion of pure water and spores is determined by two factors, namely the concentration requirement of the stock solution after spore wall breaking and various parameters of the maximum output light energy of the laser set by the process.
Referring to fig. 1, 3, 4 and 5, the pipeline pump 2 is used for conveying the mixed liquor in the mixing barrel 1 to the light energy receiver 4, the laser is placed on one side of the outside of the light energy receiver 4 and is used for performing laser irradiation on the mixed liquor in the light energy receiver 4, and the water pump 5 is used for conveying the mixed liquor in the light energy receiver 4 to the finished product barrel 6. In order to improve the edibility and safety of the spore application, the mixing barrel 1, the pipeline pump 2, the first flow controller 31 and the light energy receiver 4 are preferably connected through a food-grade plastic conduit 8 in sequence. The light energy receiver 4, the second flow controller 32, the water pump 5 and the finished product barrel 6 are connected in turn through a medical grade plastic conduit 9. And before spore wall breaking, the spore pipeline transportation is all through food grade plastic pipe 8, and after spore wall breaking, the spore pipeline transportation is all through medical grade plastic pipe 9.
It should be noted that the electrodeless direct-current voltage speed-regulating micro pipeline pump 2 (the wading part in the pump is food-grade stainless steel) is conveyed to the light energy receiver 4 from the stainless steel mixing barrel 1 through the food-grade plastic conduit 8. The first flow monitoring controller is arranged in the middle of the pipeline, and the first flow monitoring controller and the pipeline pump 2 are controlled in a closed loop mode to ensure that the pressure/flow rate of the mixed liquid conveyed to the light energy receiver 4 meets the preset requirement.
Referring to fig. 1, 3, 4 and 5, the optical energy receptor 4 includes an optical energy receptor body 41, laser optical energy receiving areas 42 are disposed on two opposite sides of the optical energy receptor body 41, the laser optical energy receiving areas 42 are made of transparent glass, and the optical energy receptor 4 is thin and flat, so that the mixed liquid is in a water curtain wall state when passing through the optical energy receptor 4. The flow rate of the mixed liquid (i.e. the time of the mixed liquid passing through the light energy receiver 4) needs to be adapted to the type of the laser and various parameters of the laser so as to ensure the wall breaking effect. According to the properties of the outer wall and the inner wall of the medicine source plant spore and the pollen spore, the layer numbers (2 layers or 3 layers) of the wall shells of various spores and the difficulty degree of wall breaking of various spores, a plurality of light energy receivers 4 can be arranged on one spore wall breaking production line, namely, a mode that the light energy receivers 4 are sequentially connected in series is adopted, so that the spore wall breaking rate is ensured to be higher. Generally, the spore wall breaking rate can reach more than 95%. When a plurality of light energy receivers 4 are arranged, if some spores are easy to break the wall, part of lasers on the wall breaking production line can not be irradiated. One or more laser light energy receiving areas 42 may be disposed on each light energy receiver 4, and when a plurality of laser light energy receiving areas 42 are disposed, the plurality of laser light energy receiving areas 42 should be sequentially disposed along the flowing direction of the mixed liquid in the light energy receiver 4.
Referring to fig. 1, 3, 4 and 5, the food grade plastic conduit 8 in the pipeline is a circular tube, and the food grade plastic conduit 8 changes its shape from a circular tube to a flat shape at the end close to the light energy receiver 4, so that the food grade plastic conduit 8 and the light energy receiver 4 are firmly combined without water seepage. The medical grade plastic conduit 9 in the pipeline is a round pipe type, and the shape of the medical grade plastic conduit 9 close to one end of the light energy receiver 4 is changed from a round pipe type to a flat type, so that the medical grade plastic conduit 9 and the light energy receiver 4 are combined firmly and do not seep water. After the spore in the mixed solution is subjected to irradiation wall breaking by the light energy receiver 4, the spore is conveyed to a medical grade closed plastic finished product barrel 6 (without oxidation in the whole process) through a medical grade plastic guide pipe 9, subpackaged to a small sealed barrel 7 suitable for transportation, and then refrigerated.
Referring to fig. 1, 3, 4, 5, the laser is a nanosecond or picosecond laser; the laser comprises one or more of a titanium gem laser, an ultraviolet-visible-near infrared band laser, an excimer laser and an ytterbium-doped fiber laser. The method comprises irradiating medicinal plant spore and pollen spore with pulsed laser beam output by laser to break their wall shells. The invention adopts a plurality of lasers (or a single laser) to combine a proper light beam irradiation domain (point region of laser emission light beam) on the laser light energy receiving region 42 of the light energy receiver 4, and spores in mixed liquid passing through the domain can receive the light energy of the laser beam by utilizing the polarization function of the lasers.
It should be noted that if only a single light energy receptor 4 is on the wall breaking production line, whether a water pump 5 is installed on the outlet pipeline of the light energy receptor 4 can be selected according to the pressure and flow rate of the mixed liquid; if a plurality of light energy receivers 4 are connected in series, an electrodeless direct current voltage speed regulation micro water pump 5 (the wading part in the pump is food-grade stainless steel) is added in a pipeline of the medical grade plastic conduit 9, and a second flow monitoring controller is installed. The closed-loop control of the water pump 5 and the closed-loop control of the pipeline pump 2 must be controlled in a linkage manner. The small sealed barrel 7 suitable for transportation needs to be subpackaged from the medical grade sealed plastic finished product barrel 6 in a vacuum chamber to prevent bacteria from falling and being oxidized, and stock solution after wall breaking is ensured not to contact with the external environment.
The physical spore wall breaking process includes the following steps:
s1, adding pure water and drug source plant spores or pollen spores needing wall breaking into a food-grade stainless steel mixing barrel 1 with a stirring device, and uniformly stirring to obtain a mixed solution;
s2, conveying the mixed liquor in the mixing barrel 1 to the light energy receiver 4 through the electrodeless direct current voltage speed regulation micro pipeline pump 2, and controlling the pressure and the flow rate of the mixed liquor in a pipeline through the first flow controller 31;
s3, performing laser irradiation on the mixed solution by one or more lasers at the side part of the light energy receiver 4 to break and crush the wall of the medicinal plant spore or pollen spore needing to be broken;
s4, after the spore in the mixed solution is irradiated and broken by the light energy receptor 4, the mixed solution is conveyed to the medical-grade sealed plastic finished product barrel 6 by the water pump 5 through the medical-grade plastic conduit 9, and then the mixed solution is subpackaged in the small sealed barrel 7 for cold storage and transportation.
Example 2: referring to fig. 2, the difference between the apparatus for physically breaking walls of spores and embodiment 1 is that the apparatus for physically breaking walls of spores further includes electromagnetic valves including a first electromagnetic valve 12 and a second electromagnetic valve 13. The first electromagnetic valve 12 is arranged between the mixing barrel 1 and the pipeline pump 2, and the second electromagnetic valve 13 is arranged between the water suction pump 5 and the finished product barrel 6. First solenoid valve 12 and second solenoid valve 13 are plumbed to return mixed liquor to light energy receptacle 4 again.
In the above technical solution, if the exine of some spores is hard and hard to circulate once, the wall is broken, and on/off switches (the first electromagnetic valve 12 and the second electromagnetic valve 13) can be added at the inlet and the outlet of the process system without adding the light energy receiver 4 and the laser, so that the mixed liquor can be circulated again in the process system, i.e. the mixed liquor passes through the light energy receiver 4 again.
The difference between the method for physically breaking the wall of the spore and the embodiment 1 is that when the wall breaking rate of the spore is low, the mixed solution is circulated again in the process system, namely the mixed solution passes through the light energy receiver 4 again, so that the reflux effect is realized.
Examples of the applications
1. The physical wall breaking device and method for the spores in the embodiment 1 are adopted to perform wall breaking treatment on the pine pollen spores, a single laser is adopted to perform laser irradiation on the laser light energy receiving areas 42 of the light energy receivers 4 in the wall breaking process, one light energy receiver 4 is arranged, and one laser light energy receiving area 42 is arranged on each light energy receiver 4.
Observing the cell structures before and after pollen Pini spore wall breaking by microscope to obtain figures 6-7.
As can be seen from fig. 6-7, after the pine pollen spores are subjected to the wall-breaking treatment by using the device and method for physically breaking the walls of the spores in example 1, the walls of the pine pollen spores are broken higher, and the pine pollen spores are substantially free of non-broken pine pollen spores.
2. The physical spore wall breaking device and method in embodiment 1 are adopted to perform wall breaking treatment on the rape pollen spores, a single laser is adopted to perform laser irradiation on the laser light energy receiving area 42 of the light energy receiver 4 in the wall breaking process, one light energy receiver 4 is arranged, and the light energy receiver 4 is provided with one laser light energy receiving area 42.
The cell structures before and after the wall breaking of the rapeseed pollen spores were observed by a microscope to obtain fig. 8-9.
As can be seen from fig. 6 to 7, after the wall-breaking treatment of the rapeseed pollen spores by using the physical wall-breaking device and method of embodiment 1, the wall-breaking of the rapeseed pollen spores is high, and only a few of the rapeseed pollen spores which are not wall-broken are contained.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that several improvements and modifications without departing from the principle of the present invention will occur to those skilled in the art, and such improvements and modifications should also be construed as within the scope of the present invention.

Claims (5)

1. The utility model provides a spore physics nature broken wall device based on laser light ability which characterized in that: comprises a mixing barrel (1), a pipeline pump (2), a light energy receiver (4) and a laser;
the mixing barrel (1), the pipeline pump (2) and the light energy receiver (4) are sequentially connected through a pipeline, a spore feed hopper (10) and a pure water inlet pipe (11) are arranged on the mixing barrel (1) so that spores and pure water can be added into the mixing barrel (1) and mixed through the mixing barrel (1) to form mixed liquid;
the pipeline pump (2) is used for conveying the mixed liquor in the mixing barrel (1) to the light energy receiver (4), and the laser is used for carrying out laser irradiation on the mixed liquor in the light energy receiver (4);
the light energy receiver (4) is provided with a laser light energy receiving area (42), the laser light energy receiving area (42) is made of transparent glass, and the light energy receiver (4) is thin and flat;
the device also comprises a flow controller, a water pump (5) and a finished product barrel (6);
the flow controller comprises a first flow controller (31) and a second flow controller (32), and the mixing barrel (1), the pipeline pump (2), the first flow controller (31), the light energy receiver (4), the second flow controller (32), the water pump (5) and the finished product barrel (6) are sequentially connected through pipelines;
the mixing barrel (1), the pipeline pump (2), the first flow controller (31) and the light energy receiver (4) are connected through a food grade plastic conduit (8) in sequence;
the light energy receiver (4), the second flow controller (32), the water pump (5) and the finished product barrel (6) are connected in sequence through a medical grade plastic conduit (9);
the food-grade plastic conduit (8) is of a circular pipe shape, and one end, close to the light energy receiver (4), of the food-grade plastic conduit (8) is of a flat shape; the medical grade plastic conduit (9) is a circular tube, and one end of the medical grade plastic conduit (9) close to the light energy receiver (4) is flat.
2. The apparatus of claim 1, wherein the apparatus for physically breaking spore wall based on laser energy comprises: the mixing device is characterized by further comprising an electromagnetic valve, wherein the electromagnetic valve comprises a first electromagnetic valve (12) and a second electromagnetic valve (13), the first electromagnetic valve (12) is installed between the mixing barrel (1) and the pipeline pump (2), and the second electromagnetic valve (13) is installed between the water suction pump (5) and the finished product barrel (6);
the first solenoid valve (12) and the second solenoid valve (13) are connected by a pipeline to send the mixed liquid back to the light energy receiver (4) again.
3. The apparatus of claim 1, wherein the apparatus for physically breaking spore wall based on laser energy comprises: the spore is medicinal source plant spore or pollen spore.
4. The apparatus of claim 1, wherein the apparatus for physically breaking spore wall based on laser energy comprises: the laser is a nanosecond laser or a picosecond laser; the laser comprises one or more of a titanium gem laser, an ultraviolet-visible-near infrared band laser, an excimer laser and an ytterbium-doped fiber laser.
5. A method for physically breaking walls of spores based on laser energy, which provides the apparatus for physically breaking walls of spores based on laser energy according to claim 1, and is characterized in that: the method comprises the following steps:
s1, adding pure water and drug source plant spores or pollen spores needing wall breaking into a food-grade stainless steel mixing barrel (1) with a stirring device, and uniformly stirring to obtain a mixed solution;
s2, conveying the mixed liquor in the mixing barrel (1) to a light energy receiver (4) through an electrodeless direct current voltage speed regulation micro pipeline pump, wherein the pressure and the flow speed of the mixed liquor in a pipeline are controlled by a flow controller;
s3, performing laser irradiation on the mixed solution by one or more lasers at the side part of the light energy receiver (4) so as to break and crush the wall of the medicinal plant spore or pollen spore needing to be broken;
s4, after spores in the mixed solution are irradiated and broken walls through the light energy receiver (4), the mixed solution is conveyed to a medical-grade closed plastic finished product barrel (6) through a medical-grade plastic guide pipe (9) by a water pump (5), and then the mixed solution is subpackaged into a small sealed barrel (7) for cold storage and transportation.
CN202010930957.1A 2020-09-07 2020-09-07 Laser light energy-based spore physical wall breaking device and method Active CN112121965B (en)

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CN113522497B (en) * 2021-09-03 2023-03-17 湖南源科工程技术有限公司 A light energy receiving area that is arranged in non-oxidation laser broken wall production line of plant spore
CN113731589B (en) * 2021-09-03 2022-12-13 湖南源科工程技术有限公司 Laser light energy receiver for plant spore non-oxidation laser wall breaking
CN114798124A (en) * 2022-03-18 2022-07-29 湖南源科工程技术有限公司 Preparation device and method of spore water aqua based on laser irradiation non-oxidation wall breaking technology

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