CN101697981A - Fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore - Google Patents
Fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore Download PDFInfo
- Publication number
- CN101697981A CN101697981A CN200910067101A CN200910067101A CN101697981A CN 101697981 A CN101697981 A CN 101697981A CN 200910067101 A CN200910067101 A CN 200910067101A CN 200910067101 A CN200910067101 A CN 200910067101A CN 101697981 A CN101697981 A CN 101697981A
- Authority
- CN
- China
- Prior art keywords
- breaking
- solution
- electric field
- ganoderma lucidum
- lucidum spore
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 21
- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 8
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 8
- 230000005684 electric field Effects 0.000 claims abstract description 17
- 239000002253 acid Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 3
- 241000222336 Ganoderma Species 0.000 claims description 26
- 239000011259 mixed solution Substances 0.000 claims description 16
- 230000001413 cellular effect Effects 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 12
- JEIPFZHSYJVQDO-UHFFFAOYSA-N ferric oxide Chemical compound O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims description 6
- 230000001476 alcoholic effect Effects 0.000 claims description 3
- 210000002421 cell wall Anatomy 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 3
- 230000000975 bioactive effect Effects 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 1
- 239000003513 alkali Substances 0.000 abstract 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 239000004615 ingredient Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 241001480597 Ganoderma tsugae Species 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 229920002101 Chitin Polymers 0.000 description 1
- 241001149422 Ganoderma applanatum Species 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- TWNIBLMWSKIRAT-VFUOTHLCSA-N levoglucosan Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@H]2CO[C@@H]1O2 TWNIBLMWSKIRAT-VFUOTHLCSA-N 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000012476 oxidizable substance Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore, belonging to the technical field of biology. The method comprises the steps of: 1. adding the ganoderma lucidum spore into water or 20-100% alcohol-containing solution or edible acid solution with the pH value of 3-7 or edible alkali solution with the pH value of 7-10, and evenly stirring to prepare mixture, wherein the weight ratio of the ganoderma lucidum spore to the solution is 1:5-15:2; and 2. processing the mixture under normal temperature though high-pressure pulse electric field to break, wherein the electric-field intensity is 10-110 kilovolt/cm and the number of the pulse is 1-800. By using the high-pressure pulse electric field, the method has less heat energy in the process of breaking, low raised temperature of a breaking target, and short breaking time. The breaking mechanism is not breaking the cell wall in the high-pressure pulse electric field but breaking the cell wall by forming a plurality of through holes on the surface of the cell wall by polar molecules in the solution under the function of the high-pressure pulse electric field, thereby improving the use ratio of bioactive substances.
Description
Technical field
The invention belongs to biological technical field.
Background technology
At present Ganoderma has been widely used in products such as medicine, food, beauty treatment, and Ganoderma spore is the elite of Ganoderma, also is anticancer in recent years and one of the popular medicine of enhancing human body immunity function.The sporoderm (polysaccharide wall) that the Ganoderma spore wall haves three layers and is made of chitin and glucosan, strong but pliable in texture, acid and alkali-resistance, therefore extremely difficult oxidation Decomposition has limited people's digesting and assimilating active substance in the spore.Most of activated report explanation, the main active substances of Ganoderma concentrates on Ganoderma spore, and contained effective ingredient and medical value, nutritive value all exceed the Ganoderma sporophore several times.Biological activity characteristic such as wherein have antitumor, protect the liver Ganoderma (Ganoderma lucidum), Ganoderma tsugae (Ganoderma tsugae), Ganoderma applanatum (Pers.) Pat (Ganoderma applanaturn) etc. are arranged.Have only the effective ingredient of the Ganoderma spore of breaking cellular wall just can be easy to the absorption of human body utilization, just embody its exclusive drug value and nutritive value.
At present, the method that domestic breaking trachytectum of glossy ganoderma is commonly used has:
1, fermentation method: during with Ganoderma spore heap fermentation to 70 ℃, its conidial cell wall begins to burst.This method is simple, but the effective ingredient major part of inclusions is hydrolyzed destruction, and sporoderm-broken rate low, pollution arranged.
2. enzymatic isolation method: adopt multiple lywallzyme to mix, in water, the Ganoderma spore wall is dissolved.Shortcoming is that the time is short, and wall does not break, and the time is long, and wall has dissolved, and its effective solute has also dissolved.Because the activity of lywallzyme is subjected to the influence of external factors such as temperature, humidity bigger, the suitable molten wall time is difficult to grasp.
3. high-frequency ultrasonic method: adopt Wet-process wall breaking, power consumption is big.
4. the high power high velocity air grinds method: need-35 ℃ low temperature environment, investment is big, the noise height, and volume of production is little, and the Ganoderma spore behind the breaking cellular wall needs cryopreservation, and production cost is higher.
5. microwave negative pressure method: adopt Wet-process wall breaking, pumped down, volume of production is little, and investment is big, the production cost height.
6. carbon dioxide steam explosion method consumes energy greatly, and is dangerous to heat-sensitive materials.
Summary of the invention
The objective of the invention is: a kind of fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore is provided, and it has simplified technology, and heat-sensitive ingredients is not subject to destruction, the volatile ingredient loss is little, and readily oxidizable substance is damaged little, and broken time is short.
Method of the present invention is:
1, Ganoderma spore is added to water contain 20～100% alcoholic solution or the edible acid solution of pH value 3～7 or pH value 7～10 edible base solution in stir evenly and produce mixed solution, Ganoderma spore is 1 with above-mentioned solution weight ratio: 5-15;
2, mixed solution is obtained breaking cellular wall by high voltage pulse electric field processing at normal temperatures, its electric field intensity is that 10-100 kv/cm, umber of pulse are 1～800.
The invention has the beneficial effects as follows: utilize the high-pressure pulse electric breaking cellular wall, heat production is few in the breaking cellular wall process, the intensification of breaking cellular wall object is little, broken time short.Breaking cellular wall mechanism be not high-pressure pulse electric with the cell wall fragmentation, but the polar molecule in the solution is under the effect of high-pressure pulse electric, the cell wall surface forms the many places perforation, thereby thin wall can be destroyed, and improves the bioactive substance utilization rate.
Material temperature increasing degree before and after high-pressure pulse electric is very little, generally in 0～30 ℃.Can protect the heat-sensitive substance in the active ingredient not to be damaged to greatest extent, this method is suitable for industrial continuous production.
The specific embodiment
Embodiment 1:
Ganoderma spore is taken by weighing 50 grams, add 500 grams to and contain in 50% alcoholic solution, make the mixed solution of 1: 10 weight ratio.
Mixed solution is obtained breaking cellular wall by high voltage pulse electric field processing at normal temperatures, and its electric field intensity is that 20-80 kv/cm, umber of pulse are 200～700.
Produce out the sporoderm-broken Ganoderma spore mixed solution of 550 grams.
Embodiment 2:
Ganoderma spore is taken by weighing 50 grams, add 500 grams to and contain in 10% citric acid solution, the pH value of citric acid is made the mixed solution of 1: 10 weight ratio 3.
Mixed solution is obtained breaking cellular wall by high voltage pulse electric field processing at normal temperatures, and its electric field intensity is that 20-80 kv/cm, umber of pulse are 100～700.
Produce out the sporoderm-broken Ganoderma spore mixed solution of 550 grams.
Embodiment 3:
Ganoderma spore is taken by weighing 50 grams, add 600 grams to and contain in 10% sodium bicarbonate solution, the pH value of sodium bicarbonate is made the mixed solution of 1: 10 weight ratio between 8-10.
Mixed solution is obtained breaking cellular wall by high voltage pulse electric field processing at normal temperatures, and its electric field intensity is that 20-80 kv/cm, umber of pulse are 100～700.
Produce out the sporoderm-broken Ganoderma spore mixed solution of 650 grams.
Embodiment 4:
Ganoderma spore is taken by weighing 50 grams, add in the 500 gram aqueous solutions, make the mixed solution of 1: 10 weight ratio.
Mixed solution is obtained breaking cellular wall by high voltage pulse electric field processing at normal temperatures, and its electric field intensity is that 20-80 kv/cm, umber of pulse are 10～700.
Produce out the sporoderm-broken Ganoderma spore mixed solution of 550 grams.
Claims (1)
1. fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore, its method is:
A, add Ganoderma spore to water or contain 20～100% alcoholic solution or the edible acid solution of pH value 3～7 or pH value 7～10 edible base solution in stir evenly and produce mixed solution, Ganoderma spore is 1 with above-mentioned solution weight ratio: 5-15;
B, mixed solution is obtained breaking cellular wall by high voltage pulse electric field processing at normal temperatures, its electric field intensity is that 10-100 kv/cm, umber of pulse are 1～800.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910067101XA CN101697981B (en) | 2009-06-12 | 2009-06-12 | Fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910067101XA CN101697981B (en) | 2009-06-12 | 2009-06-12 | Fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101697981A true CN101697981A (en) | 2010-04-28 |
| CN101697981B CN101697981B (en) | 2011-11-23 |
Family
ID=42146445
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910067101XA Active CN101697981B (en) | 2009-06-12 | 2009-06-12 | Fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101697981B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555709A (en) * | 2013-10-22 | 2014-02-05 | 哈尔滨师范大学 | Algae cell breaking method |
| CN103923226A (en) * | 2014-04-23 | 2014-07-16 | 赛珂睿德生物医药科技(上海)有限公司 | Method for pretreating raw material for preparing pectin |
| CN104095175A (en) * | 2014-07-31 | 2014-10-15 | 中国农业科学院特产研究所 | Ginseng royal jelly granules with anti-radiation effect |
| CN109157550A (en) * | 2018-09-14 | 2019-01-08 | 广东润源中天生物科技有限公司 | A kind of acid infiltration high pressure frost wall breaking technology of lucidum spore powder |
| CN111329054A (en) * | 2020-03-10 | 2020-06-26 | 广东健微营养科技有限公司 | A nutritional preparation containing Haematococcus pluvialis and its preparation method |
| CN112121965A (en) * | 2020-09-07 | 2020-12-25 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
-
2009
- 2009-06-12 CN CN200910067101XA patent/CN101697981B/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555709A (en) * | 2013-10-22 | 2014-02-05 | 哈尔滨师范大学 | Algae cell breaking method |
| CN103923226A (en) * | 2014-04-23 | 2014-07-16 | 赛珂睿德生物医药科技(上海)有限公司 | Method for pretreating raw material for preparing pectin |
| CN103923226B (en) * | 2014-04-23 | 2016-08-24 | 赛珂睿德生物医药科技(上海)有限公司 | A kind of preprocessing method of raw materials prepared for pectin |
| CN104095175A (en) * | 2014-07-31 | 2014-10-15 | 中国农业科学院特产研究所 | Ginseng royal jelly granules with anti-radiation effect |
| CN109157550A (en) * | 2018-09-14 | 2019-01-08 | 广东润源中天生物科技有限公司 | A kind of acid infiltration high pressure frost wall breaking technology of lucidum spore powder |
| CN111329054A (en) * | 2020-03-10 | 2020-06-26 | 广东健微营养科技有限公司 | A nutritional preparation containing Haematococcus pluvialis and its preparation method |
| CN112121965A (en) * | 2020-09-07 | 2020-12-25 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
| CN112121965B (en) * | 2020-09-07 | 2021-12-10 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101697981B (en) | 2011-11-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101697981B (en) | Fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore | |
| Yan et al. | High intensity pulsed electric field as an innovative technique for extraction of bioactive compounds—A review | |
| Li et al. | Prospects and application of ultrasound and magnetic fields in the fermentation of rare edible fungi | |
| CN101268815B (en) | Supercritical carbonic anhydride wall-breaking method for melissa powder | |
| CN104042642A (en) | Ganoderma spore wall breaking process | |
| CN103564310B (en) | Flour product suitable for patients suffering from kidney disease and processing method thereof | |
| CN103555582B (en) | Method for breaking wall of chlorella by using compound enzyme | |
| CN101418329B (en) | Preparation method of rapeseed proteolysis peptides based on pulse ultrasonic technology and use thereof | |
| EP2859797A1 (en) | Additive composition for shrimp feed and a method for producing the same | |
| KR20140028305A (en) | A fermentation medicinal herbs extract process | |
| CN103340909B8 (en) | Biological wall breaking method for ganoderma lucidum spore powder | |
| CN106993807A (en) | A kind of preparation method of ginger ferment | |
| CN104592409A (en) | Method for extracting high-activity phellinus linteus polysaccharide at low pressure and low temperature | |
| CN104072627B (en) | A kind of preparation method of Herba Typhonii gigantei polyoses extract | |
| CN101108196A (en) | Method of producing glossy ganoderma polypeptide product | |
| CN104946628A (en) | Extraction method of mycelial fungus genome | |
| CN101579366A (en) | Method for fully releasing active ingredients of ganoderma lucidum spores | |
| CN1165032A (en) | Method for breaking cell wall of Ganoderma lucidum spore | |
| CN107779400A (en) | A kind of microorganism wall-breaking method of lucidum spore powder | |
| CN108048329B (en) | Method for breaking wall of microalgae cell | |
| CN106103681A (en) | A kind of health preserving wine and preparation method thereof | |
| CN101549928A (en) | Ultrasonic ultraviolet algea-allelopathy sterilizing unit | |
| CN100445378C (en) | Ultrasonic method for breaking up micro alga cells | |
| CN204521608U (en) | Medical material is broken and decoct integrated apparatus | |
| CN103756843A (en) | Functional cranberry and blackberry composite fruit wine and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210707 Address after: 130118 No. 2888 Xincheng street, Jilin, Changchun Patentee after: JILIN AGRICULTURAL University Address before: No.15-602, Qinqing garden, 2888 Xincheng street, Changchun City, Jilin Province Patentee before: Li Changtian |