CN101697981B - Fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore - Google Patents
Fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore Download PDFInfo
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- CN101697981B CN101697981B CN200910067101XA CN200910067101A CN101697981B CN 101697981 B CN101697981 B CN 101697981B CN 200910067101X A CN200910067101X A CN 200910067101XA CN 200910067101 A CN200910067101 A CN 200910067101A CN 101697981 B CN101697981 B CN 101697981B
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Abstract
The invention provides a fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore, belonging to the technical field of biology. The method comprises the steps of: 1. adding the ganoderma lucidum spore into water or 20-100% alcohol-containing solution or edible acid solution with the pH value of 3-7 or edible alkali solution with the pH value of 7-10, and evenly stirring to prepare mixture, wherein the weight ratio of the ganoderma lucidum spore to the solution is 1:5-15:2; and 2. processing the mixture under normal temperature though high-pressure pulse electric field to break, wherein the electric-field intensity is 10-110 kilovolt/cm and the number of the pulse is 1-800. By using the high-pressure pulse electric field, the method has less heat energy in the process of breaking, low raised temperature of a breaking target, and short breaking time. The breaking mechanism is not breaking the cell wall in the high-pressure pulse electric field but breaking the cell wall by forming a plurality of through holes on the surface of the cell wall by polar molecules in the solution under the function of the high-pressure pulse electric field, thereby improving the use ratio of bioactive substances.
Description
Technical field
The invention belongs to biological technical field.
Background technology
At present Ganoderma has been widely used in products such as medicine, food, beauty treatment, and Ganoderma spore is the elite of Ganoderma, also is anticancer in recent years and one of the popular medicine of enhancing human body immunity function.The sporoderm (polysaccharide wall) that the Ganoderma spore wall haves three layers and is made of chitin and glucosan, strong but pliable in texture, acid and alkali-resistance, therefore extremely difficult oxidation Decomposition has limited people's digesting and assimilating active substance in the spore.Most of activated report explanation, the main active substances of Ganoderma concentrates on Ganoderma spore, and contained effective ingredient and medical value, nutritive value all exceed the Ganoderma sporophore several times.Biological activity characteristic such as wherein have antitumor, protect the liver Ganoderma (Ganoderma lucidum), Ganoderma tsugae (Ganoderma tsugae), Ganoderma applanatum (Pers.) Pat (Ganoderma applanaturn) etc. are arranged.Have only the effective ingredient of the Ganoderma spore of breaking cellular wall just can be easy to the absorption of human body utilization, just embody its exclusive drug value and nutritive value.
At present, the method that domestic breaking trachytectum of glossy ganoderma is commonly used has:
1, fermentation method: during with Ganoderma spore heap fermentation to 70 ℃, its conidial cell wall begins to burst.This method is simple, but the effective ingredient major part of inclusions is hydrolyzed destruction, and sporoderm-broken rate low, pollution arranged.
2. enzymatic isolation method: adopt multiple lywallzyme to mix, in water, the Ganoderma spore wall is dissolved.Shortcoming is that the time is short, and wall does not break, and the time is long, and wall has dissolved, and its effective solute has also dissolved.Because the activity of lywallzyme is subjected to the influence of external factors such as temperature, humidity bigger, the suitable molten wall time is difficult to grasp.
3. high-frequency ultrasonic method: adopt Wet-process wall breaking, power consumption is big.
4. the high power high velocity air grinds method: need-35 ℃ low temperature environment, investment is big, the noise height, and volume of production is little, and the Ganoderma spore behind the breaking cellular wall needs cryopreservation, and production cost is higher.
5. microwave negative pressure method: adopt Wet-process wall breaking, pumped down, volume of production is little, and investment is big, the production cost height.
6. carbon dioxide steam explosion method consumes energy greatly, and is dangerous to heat-sensitive materials.
Summary of the invention
The objective of the invention is: a kind of fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore is provided, and it has simplified technology, and heat-sensitive ingredients is not subject to destruction, the volatile ingredient loss is little, and readily oxidizable substance is damaged little, and broken time is short.
Method of the present invention is:
1, Ganoderma spore is added to water contain 20～100% alcoholic solution or the edible acid solution of pH value 3～7 or pH value 7～10 edible base solution in stir evenly and produce mixed solution, Ganoderma spore is 1 with above-mentioned solution weight ratio: 5-15;
2, mixed solution is obtained breaking cellular wall by high voltage pulse electric field processing at normal temperatures, its electric field intensity is that 10-100 kv/cm, umber of pulse are 1～800.
The invention has the beneficial effects as follows: utilize the high-pressure pulse electric breaking cellular wall, heat production is few in the breaking cellular wall process, the intensification of breaking cellular wall object is little, broken time short.Breaking cellular wall mechanism be not high-pressure pulse electric with the cell wall fragmentation, but the polar molecule in the solution is under the effect of high-pressure pulse electric, the cell wall surface forms the many places perforation, thereby thin wall can be destroyed, and improves the bioactive substance utilization rate.
Material temperature increasing degree before and after high-pressure pulse electric is very little, generally in 0 ~ 30 ℃.Can protect the heat-sensitive substance in the active ingredient not to be damaged to greatest extent, this method is suitable for industrial continuous production.
The specific embodiment
Embodiment 1:
Ganoderma spore is taken by weighing 50 grams, add 500 grams to and contain in 50% alcoholic solution, make the mixed solution of 1: 10 weight ratio.
Mixed solution is obtained breaking cellular wall by high voltage pulse electric field processing at normal temperatures, and its electric field intensity is that 20-80 kv/cm, umber of pulse are 200～700.
Produce out the sporoderm-broken Ganoderma spore mixed solution of 550 grams.
Embodiment 2:
Ganoderma spore is taken by weighing 50 grams, add 500 grams to and contain in 10% citric acid solution, the pH value of citric acid is made the mixed solution of 1: 10 weight ratio 3.
Mixed solution is obtained breaking cellular wall by high voltage pulse electric field processing at normal temperatures, and its electric field intensity is that 20-80 kv/cm, umber of pulse are 100～700.
Produce out the sporoderm-broken Ganoderma spore mixed solution of 550 grams.
Embodiment 3:
Ganoderma spore is taken by weighing 50 grams, add 600 grams to and contain in 10% sodium bicarbonate solution, the pH value of sodium bicarbonate is made the mixed solution of 1: 10 weight ratio between 8-10.
Mixed solution is obtained breaking cellular wall by high voltage pulse electric field processing at normal temperatures, and its electric field intensity is that 20-80 kv/cm, umber of pulse are 100～700.
Produce out the sporoderm-broken Ganoderma spore mixed solution of 650 grams.
Embodiment 4:
Ganoderma spore is taken by weighing 50 grams, add in the 500 gram aqueous solutions, make the mixed solution of 1: 10 weight ratio.
Mixed solution is obtained breaking cellular wall by high voltage pulse electric field processing at normal temperatures, and its electric field intensity is that 20-80 kv/cm, umber of pulse are 10～700.
Produce out the sporoderm-broken Ganoderma spore mixed solution of 550 grams.
Claims (1)
1. fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore, its method is:
A, add Ganoderma spore to water or contain 20～100% alcoholic solution or the edible acid solution of pH value 3～7 or pH value 7～10 edible base solution in stir evenly and produce mixed solution, Ganoderma spore is 1 with above-mentioned solution weight ratio: 5-15;
B, mixed solution is obtained breaking cellular wall by high voltage pulse electric field processing at normal temperatures, its electric field intensity is that 20-80 kv/cm, umber of pulse are 10～700.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN200910067101XA CN101697981B (en) | 2009-06-12 | 2009-06-12 | Fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore |
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| CN200910067101XA CN101697981B (en) | 2009-06-12 | 2009-06-12 | Fast, non-heat change and high-efficiency method for breaking ganoderma lucidum spore |
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| CN101697981A CN101697981A (en) | 2010-04-28 |
| CN101697981B true CN101697981B (en) | 2011-11-23 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103555709A (en) * | 2013-10-22 | 2014-02-05 | 哈尔滨师范大学 | Algae cell breaking method |
| CN103923226B (en) * | 2014-04-23 | 2016-08-24 | 赛珂睿德生物医药科技(上海)有限公司 | A kind of preprocessing method of raw materials prepared for pectin |
| CN104095175B (en) * | 2014-07-31 | 2016-05-25 | 中国农业科学院特产研究所 | A kind of ginseng royal jelly's particle with anti-radiation effect |
| CN109157550A (en) * | 2018-09-14 | 2019-01-08 | 广东润源中天生物科技有限公司 | A kind of acid infiltration high pressure frost wall breaking technology of lucidum spore powder |
| CN111329054B (en) * | 2020-03-10 | 2022-07-26 | 广东健微营养科技有限公司 | A nutritional preparation containing Haematococcus pluvialis and its preparation method |
| CN112121965B (en) * | 2020-09-07 | 2021-12-10 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
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Effective date of registration: 20210707 Address after: 130118 No. 2888 Xincheng street, Jilin, Changchun Patentee after: JILIN AGRICULTURAL University Address before: No.15-602, Qinqing garden, 2888 Xincheng street, Changchun City, Jilin Province Patentee before: Li Changtian |