CN107080851A - A kind of ethanol freezing sterilizing methods of cordyceps sinensis and its application - Google Patents
A kind of ethanol freezing sterilizing methods of cordyceps sinensis and its application Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
- A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
- A61L2/0082—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using chemical substances
- A61L2/0088—Liquid substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/20—Targets to be treated
- A61L2202/21—Pharmaceuticals, e.g. medicaments, artificial body parts
Abstract
A kind of ethanol freezing sterilizing methods of cordyceps sinensis and its application, comprise the following steps：（1）Infiltration：Cordyceps sinensis is crushed to 60 1000 mesh, 70 85% ethanol infiltration 0.5 5h of processing is added, the volumes of aqueous ethanol is 15 times of cordyceps sinensis weight, it is 5 35% to add pure water and be diluted to concentration of alcohol；（2）Pre-freeze：Solution after dilution is spread into disk, it is 65-50 DEG C of 6h of pre-freeze 2 to insert temperature；（3）Vacuum freeze drying：In 45-20 DEG C of 30h of lyophilization 10, the μ bar of vacuum 100 400；The parsing-desiccation at least 2h at 15 20 DEG C, the μ bar of vacuum 10 100, that is, obtain cordyceps sinensis sterilizing freeze-dried powder, the freeze-dried powder total plate count killing rate ＞ 99%；Simple to operate, safety non-toxic of the invention, it is adaptable to actual big production requirement, with good medicine and food, health products DEVELOPMENT PROSPECT.
Description
Technical field
The present invention relates to Chinese medicine and health food preparing technical field, the ethanol freezing sterilizing of specifically a kind of cordyceps sinensis
Method and its application.
Background technology
Cordyceps sinensis is Clavicipitaceae fungi, stroma and larva that aweto is colonized on Hepialidae insect larvae
The dry composite body of corpse.Property sweet taste, enter lung kidney channel, function benefit lung, kidney, cough-relieving are breathed heavily, qi-restoratives is damaged, strengthening the essence gas.For chronic cough
Void is breathed heavily, overstrain cough spitting of blood, impotence and seminal emission, soreness of waist and knee joint.《Essentials of Materia Medica》：" cordyceps sinensis, Gan Ping, guarantor's lung kidney-nourishing, hemostasis and phlegm,
Only phthisical cough ".Zhao Xuemin《A Supplement to the Compendium of Materia Medica》Record：" cordyceps sinensis it is warm-natured and, mend marrow, this thing protect lung qi.With steeping in wine number
Piece it is eaten, control waist and knee and ask pain, there is the work(of kidney-nourishing, preferably the elderly.
Cordyceps sinensis is grown among the soil of highlands, is cladded with film, and Tibetan are early summer stroma is unearthed, spore do not dissipate
When dug out, generally directly the fibrous attachment of its outer membrane and soil impurity are brushed off with wood brush, cool place is to six or seven naturally
Cheng Gan.Whole process is simple, extensive, and cordyceps sinensis surface can carry the microorganism in soil with can not avoiding.Meanwhile, wild worm
Careless rich in nutrition content, is also easily polluted by various microorganisms in its growth, storage, causes its former powder product bacteria containing amount
More than state health standards, product quality is had a strong impact on.Cordyceps sinensis contain cordycepin, cordycepic acid, Cordyceps sinensis polysaccharide, amino acid,
The compositions such as aliphatic acid, sterol, protein and various active enzyme, wherein many materials can occur under hot and humid environment denaturation,
Hydrolysis causes functional component largely to lose.Therefore traditional high temperature, moist hear heat test are not suitable for cordyceps sinensis.
In prior art research and Patents, the used method that sterilized to non-refractory high moisture material mainly includes
The irradiation method of cobalt -60, Electron Beam Irradiation, ozone sterilization method, microwave sterilization method, ethylene oxide sterilizing method, superfreeze method etc.,
The presence bacterium residue problem having among these methods, what is had can then destroy the functional component of material, and some methods, which also exist, goes out
The problem of bacterium condition is harsh, sterilization effect is not good.It is micro- that such as Chinese patent publication No. discloses cordyceps sinensis for CN 104000853A
Powder preparation method and its preparation, by after crushing aweto micropowder carry out be not less than -268.7 DEG C superfreeze sterilizing and
Not higher than 65 DEG C of the freeze thawing treatment of more than 2 times repeatedly.This method is disadvantageous in that：Temperature control is to -268.7 DEG C first
It is very harsh to equipment requirement, although limit low temperature and multigelation can cause mechanical damage and the dehydration of part microbial cell
Death, but part micro organism quantity can take the mode of " self dormancy " under cryogenic, self-protection be carried out, when condition
Breeding is properly brought back to life again, is extremely difficult to expected sterilization effect.Chinese patent publication No. is that CN 104415064A disclose the winter
The fresh-keeping storage technology of worm summer grass, will carry out vacuum freeze drying, then be passed through more than 120ppm after fresh cordyceps sinensis cleaning
Ozone sterilization 60min, ozone sterilization method is limited to the penetration power of material, and directly cordyceps sinensis raw material, which is sterilized, can also make
Into a large amount of losses of wherein oxidizable composition.Chinese patent publication No. is that CN 103300276B disclose a kind of sterilizing of pollen
Method, first freezes pollen 8 ~ 12 days in -18 DEG C--20 DEG C, then with HIGH-POWERED MICROWAVES the pollen after freezing is carried out into microwave
Heating and sterilizing, line speed are 0.3 ~ 0.5m/s, and microwave power is 20 ~ 24KW；Temperature control is in 60 ~ 90 DEG C of scopes；
Again with 6 ~ 12KW microwave power on the basis of this, 10 ~ 20min of sterilizing at 85 ~ 90 DEG C.When being sterilized using microwave, go out
Bacterium effect is good, and pollen color and composition are easy to change, and influences quality；And when pollen color and composition keep preferable,
Sterilization effect is undesirable.Therefore, microwave sterilization method can not really apply to the sterilizing of pollen and cordyceps sinensis this kind of product
In production technology, it is the art urgent problem to be solved to explore a kind of sterilizing methods of suitable cordyceps sinensis powder.
The content of the invention
Present invention aim to solve above mentioned problem that existing sterilizing methods are present there is provided one kind to be applied to winter worm
The sterilizing methods of summer grass meal and its application, this method can effectively solve the problem of microorganism is exceeded serious in cordyceps sinensis powder,
And simple to operate, safety non-toxic, suitable for actual big production requirement, physics and chemistry and the life of cordyceps sinensis powder can be protected to greatest extent
Thing characteristic, improves solubility, promotes to absorb, with good medicine and food, health food development prospect.
A kind of ethanol freezing sterilizing methods of cordyceps sinensis of the present invention, comprise the following steps：
（1）Ethanol infiltrates：Cordyceps sinensis is crushed to 60-1000 mesh, 70-85% ethanol solutions is added and soaks Cordyceps sinensis powder
Profit processing 0.5-5h, the volume of the ethanol solution is 1-5 times of cordyceps sinensis weight, adds pure water and is diluted in solution
The concentration of ethanol is 5-35%；
（2）Pre-freeze：It will be inserted after solution paving disk after dilution in freeze drier, temperature is-65-- 50 DEG C, pre-freeze 2-6h；
（3）Vacuum freeze drying：Lyophilization is carried out at-45-- 20 DEG C, vacuum is 100-400 μ bar, drying time 10-
30h；The parsing-desiccation at 15-20 DEG C again, vacuum is 10-100 μ bar, drying time at least 2h, you can obtain cordyceps sinensis
Sterilize freeze-dried powder, and the water content in the cordyceps sinensis sterilizing freeze-dried powder is 1-5%, total plate count killing rate ＞ 99%.
Heretofore described step（2）In the mm of paving disc thickness≤20.
Present invention also offers a kind of application of the ethanol of cordyceps sinensis freezing sterilizing methods in Chinese herbal medicine.
Heretofore described Chinese herbal medicine is ginseng, pseudo-ginseng, cervus elaphus linnaeus, pearl, Radix Angelicae Sinensis, maca.
It is of the invention compared with existing sterilizing methods, with following advantage：
（1）Whole sterilization process only uses ethanol and pure water, any poisonous and harmful chemical reagent is not in contact with, in the absence of chemistry
Residue problem；
（2）The present invention is using the freezing sterilizing of ethanol combined vacuum, and sterilization effect is good, is not in irradiation sterilization, ozone sterilization
And the color change that causes such as microwave sterilization and chemical composition loss problem, freeze-drying sterilization process is in low temperature, vacuum state
It is lower to complete, it is to avoid the side effect such as high temperature, oxidation, enzymatic browning phenomenon caused by heat drying mode is solved, being capable of maximum journey
Prevent the denaturation in terms of physics and chemistry and biology on degree；
（3）The present invention combines the sterilization method of freezing by ethanol, can arrive the control of the micro organism quantity in cordyceps sinensis powder
Meet national food safety standard requirement, and do not result in the loss of cordyceps sinensis powder functional component adenosine content；
（4）Mode of the present invention can reach suppression by being freeze-dried mode by the moisture control of cordyceps sinensis powder to 1-5%
Microorganism processed growth, the mesh of breeding, improve the stability of product shelf life, resulting Chinese caterpillar fungus freeze-dried powder skeleton is dredged
Pine, rehydration dissolubility is improved, and promotes to absorb；
（5）Ethanol is infiltrated the present invention into sterilizing and freezing sterilizing is combined, and required equipment is common, simple to operate, can meet industry
Change production requirement；
（6）The present invention has good ductility, this method can be applied into ginseng, pseudo-ginseng, cervus elaphus linnaeus, pearl, Radix Angelicae Sinensis, agate
In the Chinese herbal medicines such as coffee.
Embodiment
Embodiment 1
A kind of ethanol freezing sterilizing methods of cordyceps sinensis of the present embodiment, comprise the following steps：
（1）Ethanol infiltrates：Cordyceps sinensis is crushed to 60 mesh, 70% ethanol solution is added by Cordyceps sinensis powder infiltration processing 5h,
The volume of the ethanol solution is 5 times of cordyceps sinensis weight, adds pure water and is diluted to the concentration of ethanol in solution and is
35%；
（2）Pre-freeze：It will insert pre-freeze 6h in -65 DEG C of freeze drier after solution paving disk after dilution, the paving disc thickness≤
20 mm；
（3）Vacuum freeze drying：Lyophilization 30h is carried out after pre-freeze at -45 DEG C, vacuum is 100 μ bar；Again at 15 DEG C
Lower parsing-desiccation at least 2h, vacuum is 10 μ bar, you can obtain cordyceps sinensis sterilizing freeze-dried powder.
The cordyceps sinensis sterilizing freeze-dried powder that the present embodiment is obtained carries out hygiene, adenosine with the cordyceps sinensis powder before sterilizing
The item detections such as content, moisture, as a result see the table below 1.
As can be seen from Table 1, the cordyceps sinensis sterilizing freeze-dried powder that the present embodiment is obtained effectively reduces cordyceps sinensis powder
The content of microorganism in end, total plate count killing rate is 99.13%, coliform killing rate ＞ 99.75%, mould killing rate ＞
99.62%, and do not result in the loss of functional component adenosine content in cordyceps sinensis.
Embodiment 2
A kind of ethanol freezing sterilizing methods of cordyceps sinensis of the present embodiment, comprise the following steps：
（1）Ethanol infiltrates：Cordyceps sinensis is crushed to 300 mesh, 75% ethanol solution is added and handles Cordyceps sinensis powder infiltration
3h, the volume of the ethanol solution is 2.5 times of cordyceps sinensis weight, adds the concentration that pure water is diluted to ethanol in solution
For 15%；
（2）Pre-freeze：It will insert pre-freeze 4h in -60 DEG C of freeze drier after solution paving disk after dilution, the paving disc thickness≤
20 mm；
（3）Vacuum freeze drying：Lyophilization 22h is carried out after pre-freeze at -35 DEG C, vacuum is 200 μ bar；Again at 18 DEG C
Lower parsing-desiccation at least 2h, vacuum is 50 μ bar, you can obtain cordyceps sinensis sterilizing freeze-dried powder.
The cordyceps sinensis sterilizing freeze-dried powder that the present embodiment is obtained carries out hygiene, adenosine with the cordyceps sinensis powder before sterilizing
The item detections such as content, moisture, as a result see the table below 2.
As can be seen from Table 2, the cordyceps sinensis sterilizing freeze-dried powder that the present embodiment is obtained effectively reduces cordyceps sinensis powder
The content of microorganism in end, total plate count killing rate ＞ 99.93%, coliform killing rate ＞ 99.23%, mould killing rate ＞
99.72%, and do not result in the loss of functional component adenosine content in cordyceps sinensis.
Embodiment 3
A kind of ethanol freezing sterilizing methods of cordyceps sinensis of the present embodiment, comprise the following steps：
（1）Ethanol infiltrates：Cordyceps sinensis is crushed to 1000 mesh, 85% ethanol solution is added and handles Cordyceps sinensis powder infiltration
0.5h, the volume of the ethanol solution is 1 times of cordyceps sinensis weight, adds the concentration that pure water is diluted to ethanol in solution
For 5%；
（2）Pre-freeze：It will insert pre-freeze 2h in -50 DEG C of freeze drier after solution paving disk after dilution, the paving disc thickness≤
20 mm；
（3）Vacuum freeze drying：Lyophilization 10h is carried out after pre-freeze at -20 DEG C, vacuum is 400 μ bar；Again at 20 DEG C
Lower parsing-desiccation at least 2h, vacuum is 100 μ bar, you can obtain cordyceps sinensis sterilizing freeze-dried powder.
The cordyceps sinensis sterilizing freeze-dried powder that the present embodiment is obtained carries out hygiene, adenosine with the cordyceps sinensis powder before sterilizing
The item detections such as content, moisture, as a result see the table below 3.
As can be seen from Table 3, the cordyceps sinensis sterilizing freeze-dried powder that the present embodiment is obtained effectively reduces cordyceps sinensis powder
The content of microorganism in end, total plate count killing rate ＞ 99.91%, coliform killing rate ＞ 99.68%, mould killing rate ＞
99.78%, and do not result in the loss of functional component adenosine content in cordyceps sinensis.
Embodiment 4
A kind of application of the ethanol freezing sterilizing methods of cordyceps sinensis of the present embodiment in maca, comprises the following steps：
（1）Ethanol infiltrates：Maca is crushed to 800 mesh, 75% ethanol solution is added by maca powder infiltration processing 1h, the second
The volume of alcoholic solution is 3 times of maca weight, and it is 10% to add pure water and be diluted to the concentration of ethanol in solution；
（2）Pre-freeze：It will insert pre-freeze 5h in -55 DEG C of freeze drier after solution paving disk after dilution, the paving disc thickness≤
20 mm；
（3）Vacuum freeze drying：Lyophilization 16h is carried out after pre-freeze at -25 DEG C, vacuum is 300 μ bar；Again at 20 DEG C
Lower parsing-desiccation at least 2h, vacuum is 20 μ bar, you can obtain maca sterilizing freeze-dried powder.
The maca sterilizing freeze-dried powder that the present embodiment is obtained carries out hygiene, benzyl mustard oil glycosides with the pueraria root powder before sterilizing
The item detections such as content, moisture, as a result see the table below 4.
As can be seen from Table 4, the maca sterilizing freeze-dried powder that the present embodiment is obtained effectively reduces micro- life in maca powder
The content of thing, total plate count killing rate is 99.30%, coliform killing rate ＞ 99.41%, mould killing rate ＞ 99.09%, and
The loss of functional component benzyl mustard oil glycosides content in maca is not resulted in.
Embodiment 5
A kind of application of the ethanol freezing sterilizing methods of cordyceps sinensis of the present embodiment in Radix Angelicae Sinensis, comprises the following steps：
（1）Ethanol infiltrates：Radix Angelicae Sinensis is crushed to 800 mesh, 75% ethanol solution is added by Chinese Angelica Root infiltration processing 2h, the second
The volume of alcoholic solution is 2 times of Radix Angelicae Sinensis weight, and it is 10% to add pure water and be diluted to the concentration of ethanol in solution；
（2）Pre-freeze：It will insert pre-freeze 5h in -55 DEG C of freeze drier after solution paving disk after dilution, the paving disc thickness≤
20 mm；
（3）Vacuum freeze drying：Lyophilization 20h is carried out after pre-freeze at -35 DEG C, vacuum is 200 μ bar；Again at 20 DEG C
Lower parsing-desiccation at least 2h, vacuum is 50 μ bar, you can obtain Radix Angelicae Sinensis sterilizing freeze-dried powder.
The Radix Angelicae Sinensis sterilizing freeze-dried powder that the present embodiment is obtained and the angelica powder before sterilizing carry out hygiene, ferulaic acid content,
The item detections such as moisture, as a result see the table below 5.
As can be seen from Table 5, the Radix Angelicae Sinensis sterilizing freeze-dried powder that the present embodiment is obtained effectively reduces micro- life in Chinese Angelica Root
The content of thing, total plate count killing rate is 99.02%, coliform killing rate ＞ 99.17%, and have no in Radix Angelicae Sinensis functional component Ah
The significantly sacrificing of Wei's acid content.
Claims (4)
1. the ethanol freezing sterilizing methods of a kind of cordyceps sinensis, it is characterised in that comprise the following steps：
（1）Ethanol infiltrates：Cordyceps sinensis is crushed to 60-1000 mesh, 70-85% ethanol solutions is added and soaks Cordyceps sinensis powder
Profit processing 0.5-5h, the volume of the ethanol solution is 1-5 times of cordyceps sinensis weight, adds pure water and is diluted in solution
The concentration of ethanol is 5-50%；
（2）Pre-freeze：It will be inserted after solution paving disk after dilution in freeze drier, temperature is-65-- 50 DEG C, pre-freeze 2-6h；
（3）Vacuum freeze drying：Lyophilization is carried out at-45-- 20 DEG C, vacuum is 100-400 μ bar, drying time 10-
30h；The parsing-desiccation at 15-20 DEG C again, vacuum is 10-100 μ bar, drying time at least 2h, you can obtain cordyceps sinensis
Sterilize freeze-dried powder, and the water content in the cordyceps sinensis sterilizing freeze-dried powder is 1-5%, total plate count killing rate ＞ 99%.
2. a kind of ethanol freezing sterilizing methods of cordyceps sinensis according to claim 1, it is characterised in that：The step
（2）In the mm of paving disc thickness≤20.
3. a kind of application of the ethanol freezing sterilizing methods of cordyceps sinensis as claimed in claim 1 in Chinese herbal medicine.
4. a kind of application of the ethanol freezing sterilizing methods of cordyceps sinensis according to claim 3 in Chinese herbal medicine, it is special
Levy and be：The Chinese herbal medicine is ginseng, pseudo-ginseng, cervus elaphus linnaeus, pearl, Radix Angelicae Sinensis, maca.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110632322A (en) * | 2019-08-27 | 2019-12-31 | 迪瑞医疗科技股份有限公司 | Freeze-drying preparation process of apolipoprotein C2 calibrator |
| CN111420084A (en) * | 2020-03-23 | 2020-07-17 | 贵阳学院 | Method for killing pathogenic bacteria (PSA) of kiwi fruit pollen canker in vacuum by using low-temperature microwaves |
| CN115429819A (en) * | 2022-10-19 | 2022-12-06 | 云南中医药大学 | Method for processing and preparing high-activity leech powder |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1480197A (en) * | 2003-06-30 | 2004-03-10 | 李建立 | Method for preparing oral liquid of Chinese herbal medicine by using attritioning technique |
| CN1562186A (en) * | 2004-03-17 | 2005-01-12 | 北京奇源益德药物研究所 | Preparation of Chinese traditional medicine for curing Illness of Cardiac cerebral ischemia and preparation method |
| CN101129414A (en) * | 2007-09-10 | 2008-02-27 | 王辉 | Chinese caterpillar fungus freeze-dried powder produced by artificial zymosis method and method thereof |
| CN101129415A (en) * | 2007-09-10 | 2008-02-27 | 王辉 | Refined Chinese caterpillar fungus powder and method of preparing the same |
| CN103305455A (en) * | 2013-06-05 | 2013-09-18 | 林树芳 | Taxus chinensis plant stem cell line breeding and anticancer drug extracting and preparing method |
| CN104415064A (en) * | 2013-08-20 | 2015-03-18 | 齐生伟 | Fresh-preserving and storage technology of cordyceps sinensis |
-
2017
- 2017-05-04 CN CN201710306339.8A patent/CN107080851B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1480197A (en) * | 2003-06-30 | 2004-03-10 | 李建立 | Method for preparing oral liquid of Chinese herbal medicine by using attritioning technique |
| CN1562186A (en) * | 2004-03-17 | 2005-01-12 | 北京奇源益德药物研究所 | Preparation of Chinese traditional medicine for curing Illness of Cardiac cerebral ischemia and preparation method |
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| CN103305455A (en) * | 2013-06-05 | 2013-09-18 | 林树芳 | Taxus chinensis plant stem cell line breeding and anticancer drug extracting and preparing method |
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| CN110632322A (en) * | 2019-08-27 | 2019-12-31 | 迪瑞医疗科技股份有限公司 | Freeze-drying preparation process of apolipoprotein C2 calibrator |
| CN110632322B (en) * | 2019-08-27 | 2021-07-13 | 迪瑞医疗科技股份有限公司 | Freeze-drying preparation process of apolipoprotein C2 calibrator |
| CN111420084A (en) * | 2020-03-23 | 2020-07-17 | 贵阳学院 | Method for killing pathogenic bacteria (PSA) of kiwi fruit pollen canker in vacuum by using low-temperature microwaves |
| CN115429819A (en) * | 2022-10-19 | 2022-12-06 | 云南中医药大学 | Method for processing and preparing high-activity leech powder |
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