CN1197598C - Biological wall breaking technology for rape melissa powder - Google Patents
Biological wall breaking technology for rape melissa powder Download PDFInfo
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- CN1197598C CN1197598C CNB021063990A CN02106399A CN1197598C CN 1197598 C CN1197598 C CN 1197598C CN B021063990 A CNB021063990 A CN B021063990A CN 02106399 A CN02106399 A CN 02106399A CN 1197598 C CN1197598 C CN 1197598C
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- wall breaking
- pollen
- enzymolysis
- sterilization
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Abstract
The present invention relates to a biological wall breaking technology for rape melissa powder, which belongs to the technical field of a production technology of medicines and health products. The biological wall breaking technology is characterized in that the technological process comprises two steps of sterilization of a pollen solution and wall breaking by enzymolysis. The biological wall breaking technology has the technological conditions that in the step of sterilization of a pollen solution, the sterilizing time is from 5 to 200 minutes; in the step of wall breaking by enzymolysis, the weight percentage concentration of the pollen solution is from 0.5 to 50%, the time for wall breaking by enzymolysis is from 0.5 to 72 hours; the temperature for wall breaking by enzymolysis is from 25 to 60 DEG C. The biological wall breaking technology has the advantages of simple and reliable manufacturing process, little investment, high efficiency of wall breaking, no damage to the nutritive substances of pollen, etc.
Description
One, technical field: the invention belongs to medicine, technical field of health care products, particularly biological wall breaking technology for rape melissa powder.
Two, technical background: Brassica campestris L pollen is the male sex-cell of rape flower, is the elite of Pollen Brassicae campestris life.Nutrient substance is very abundant in the Brassica campestris L pollen, A wide selection of colours and designs, and edibility and human body conditioning functions are high, but there is the hard conidial cell wall of one deck its outside, has seriously hindered human body to the nutraceutical absorption in its inside.Wall-breaking method at present both at home and abroad commonly used mostly is the physics method, as the high pressure draught impaction, and supercritical ultrasonics technology, freezing method heating etc., but these methods are to the equipment requirements height, and investment is big, should not promote and industrialized implementation.
Three, summary of the invention: the objective of the invention is the deficiency at above-mentioned existing cole melissa powder wall breaking method existence, design a kind of new wall-breaking method, it is simple that this method has technology, equipment requirements is not high, small investment, sporoderm-broken rate height, do not damage inner nutrient substance, the excellent popularization using value is arranged.
Technical scheme is: the technical characterictic of this Brassica campestris L pollen biological wall breaking method is that its technological process is finished for the sterilization of pollen solution, enzymolysis breaking cellular wall two steps; Process conditions are that the sterilizing time of pollen solution sterilization process is 5-200 minute, and the weight percent concentration of pollen solution is 0.5-50% in the enzymolysis breaking cellular wall operation, and the enzymolysis broken time is 0.5-72 hour, and enzymolysis breaking cellular wall temperature is 25-60 ℃.Pollen solution sterilization process is Brassica campestris L pollen to be added entry be mixed with the operation of killing various pathogenic bacterias behind the pollen solution again, and this pollen solution is pasty state solution, and sterilization can be adopted dry-air sterilization, wet heat sterilization, the sterilization of ethanol spray or other sterilization mode.Dry-air sterilization is to sterilize with pyritous air, since the temperature height, nutrient substance in the destructible pollen.Comparatively ideal sterilization mode adopts wet heat sterilization and ethanol spray sterilization mode.The wet heat sterilization mode is with high-temperature water vapor pollen solution to be carried out the mode of steam sterilization, and the sterilization temperature of wet heat sterilization mode is 100-135 ℃, and the sterilizing time of wet heat sterilization mode is 5-80 minute.Ethanol spray sterilization mode is to spray with alcoholic solution to carry out germ-resistant mode in the pollen solution.Enzymolysis breaking cellular wall operation is to through the fermentation enzymolysis pollen being carried out broken wall treatment in the pollen solution with enzyme bacterium (strain), Brassica campestris L pollen sporoderm-broken rate behind enzymolysis breaking cellular wall operation breaking cellular wall can reach more than 90%, its product is the breaking cellular wall Brassica campestris L pollen, and is direct-edible or use as additive in beverage, food, the health product.Enzyme strain in the enzymolysis breaking cellular wall operation preferably selects for use inulinase genus, the female genus of enzyme and Denmark to produce three kinds of leavens of powerful compound enzyme VlscozymeL.In the use these enzyme bacterium are mixed thoroughly and keep uniform temperature to ferment in the pollen solution, just can finish enzymolysis breaking cellular wall operation to certain hour.The weight percent concentration of pollen solution is meant the Brassica campestris L pollen parts by weight that contain in the cole melissa amidin of 100 parts of weight in the enzymolysis breaking cellular wall operation.Too dense or too rare then its shell-broken effect of concentration is all undesirable.
Biological wall breaking technology of the present invention has simple and reliable process, and equipment requirements is not high, small investment, and the sporoderm-broken rate height does not damage the pollens nutrition material, and better economic and social benefit is arranged.
Four, the specific embodiment: 1, get 5 kilograms of Brassica campestris L pollens, with 40 kilograms of pure water mixings is pollen solution, pollen solution sterilization process adopts the wet heat sterilization mode, steam the smoked kill bacterium with water vapour down at 105 ℃ and promptly finished in 30 minutes, the weight percent concentration of pollen solution elects 6% as in the enzymolysis breaking cellular wall operation.The enzyme strain selects the Saccharomyces strain to mix thoroughly at 35 ℃ of bottom fermentations can obtain the breaking cellular wall Brassica campestris L pollen solution product of sporoderm-broken rate more than 90% in 24 hours.
2, getting 20 kilograms of Brassica campestris L pollens after the medical alcohol spray is handled, is pollen solution with 80 kilograms of pure water mixings.The weight percent concentration of pollen solution is 20% in the enzymolysis breaking cellular wall operation, and it is leaven that the enzyme strain selects Denmark to produce powerful compound enzyme VlscozymeL, mixes the breaking cellular wall Brassica campestris L pollen solution product that can obtain 90% above sporoderm-broken rate at 45 ℃ of bottom fermentations in 20 hours thoroughly.
Claims (2)
1, a kind of Brassica campestris L pollen biological wall breaking method, the technological process that it is characterized in that it is finished for the sterilization of pollen solution, enzymolysis breaking cellular wall two steps, process conditions are that the sterilizing time of pollen solution sterilization process is 5-200 minute, the weight percent concentration of pollen solution is 0.5-50% in the enzymolysis breaking cellular wall operation, the enzymolysis broken time is 0.5-72 hour, and enzymolysis breaking cellular wall temperature is 25-60 ℃.
2, Brassica campestris L pollen biological wall breaking method according to claim 1, it is characterized in that pollen solution sterilization process adopts wet heat sterilization or ethanol spray sterilization mode, the sterilization temperature of wet heat sterilization mode is 100-135 ℃, and the sterilizing time of wet heat sterilization mode is 5-80 minute.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021063990A CN1197598C (en) | 2002-02-28 | 2002-02-28 | Biological wall breaking technology for rape melissa powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021063990A CN1197598C (en) | 2002-02-28 | 2002-02-28 | Biological wall breaking technology for rape melissa powder |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1377654A CN1377654A (en) | 2002-11-06 |
| CN1197598C true CN1197598C (en) | 2005-04-20 |
Family
ID=4740222
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021063990A Expired - Fee Related CN1197598C (en) | 2002-02-28 | 2002-02-28 | Biological wall breaking technology for rape melissa powder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1197598C (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101401821B (en) * | 2007-10-06 | 2011-10-05 | 徐春华 | Bee pollen peptide slice and preparation method thereof |
| CN101999568A (en) * | 2010-09-21 | 2011-04-06 | 湖北国力生物技术开发有限公司 | Biological wall breaking technology of pine pollen |
| CN102389069A (en) * | 2011-11-14 | 2012-03-28 | 西华大学 | Bee pollen cell-wall breaking method, bee pollen oral solution and its preparation method |
| CN102772345B (en) * | 2012-06-29 | 2013-12-18 | 安徽省王巢食品有限公司 | All-natural bee pollen facial mask paste and production method thereof |
| CN105360873A (en) * | 2015-11-22 | 2016-03-02 | 威海百合生物技术股份有限公司 | Quick sterilization method of spirulina powder |
| CN107468835A (en) * | 2017-09-02 | 2017-12-15 | 三株福尔制药有限公司 | The composition of microbial fermentation cattail pollen and its application |
| CN110613113A (en) * | 2019-09-24 | 2019-12-27 | 武汉市农业科学院 | Preparation method of functional biological fermentation bee pollen |
| CN110810783A (en) * | 2019-11-21 | 2020-02-21 | 青海民族大学 | Method for breaking cell wall of rape bee pollen |
-
2002
- 2002-02-28 CN CNB021063990A patent/CN1197598C/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN1377654A (en) | 2002-11-06 |
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