CN101884621B - Method for preparing salinomycin particle premix - Google Patents
Method for preparing salinomycin particle premix Download PDFInfo
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- CN101884621B CN101884621B CN2010102131911A CN201010213191A CN101884621B CN 101884621 B CN101884621 B CN 101884621B CN 2010102131911 A CN2010102131911 A CN 2010102131911A CN 201010213191 A CN201010213191 A CN 201010213191A CN 101884621 B CN101884621 B CN 101884621B
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- KQXDHUJYNAXLNZ-XQSDOZFQSA-N Salinomycin Chemical compound O1[C@@H]([C@@H](CC)C(O)=O)CC[C@H](C)[C@@H]1[C@@H](C)[C@H](O)[C@H](C)C(=O)[C@H](CC)[C@@H]1[C@@H](C)C[C@@H](C)[C@@]2(C=C[C@@H](O)[C@@]3(O[C@@](C)(CC3)[C@@H]3O[C@@H](C)[C@@](O)(CC)CC3)O2)O1 KQXDHUJYNAXLNZ-XQSDOZFQSA-N 0.000 title claims abstract description 46
- 239000004189 Salinomycin Substances 0.000 title claims abstract description 46
- 229960001548 salinomycin Drugs 0.000 title claims abstract description 46
- 235000019378 salinomycin Nutrition 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 32
- 239000002245 particle Substances 0.000 title claims abstract description 22
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 18
- 238000000855 fermentation Methods 0.000 claims abstract description 17
- 230000004151 fermentation Effects 0.000 claims abstract description 17
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 9
- 239000003513 alkali Substances 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 17
- 238000001694 spray drying Methods 0.000 claims description 13
- 241000894006 Bacteria Species 0.000 claims description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 7
- 239000002028 Biomass Substances 0.000 claims description 6
- 239000004115 Sodium Silicate Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 6
- 239000011265 semifinished product Substances 0.000 claims description 6
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052911 sodium silicate Inorganic materials 0.000 claims description 6
- 238000013337 sub-cultivation Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- 230000012010 growth Effects 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 4
- 230000002159 abnormal effect Effects 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- 230000003020 moisturizing effect Effects 0.000 claims description 3
- 230000035943 smell Effects 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 abstract description 2
- 238000005507 spraying Methods 0.000 abstract 1
- 239000000273 veterinary drug Substances 0.000 abstract 1
- ITCAUAYQCALGGV-UHFFFAOYSA-M sodium;1,4a-dimethyl-7-propan-2-yl-2,3,4,4b,5,6,10,10a-octahydrophenanthrene-1-carboxylate Chemical compound [Na+].C12CCC(C(C)C)=CC2=CCC2C1(C)CCCC2(C)C([O-])=O ITCAUAYQCALGGV-UHFFFAOYSA-M 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000008187 granular material Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical class OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 2
- 238000004134 energy conservation Methods 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 235000011121 sodium hydroxide Nutrition 0.000 description 2
- 208000003495 Coccidiosis Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010023076 Isosporiasis Diseases 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 description 1
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 150000002763 monocarboxylic acids Chemical class 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 229910001414 potassium ion Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 229910001419 rubidium ion Inorganic materials 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the veterinary drug field, in particular to a method for preparing salinomycin particle premix. The method comprises the concrete steps as follows: adjusting the pH value of salinomycin fermentation liquor with the valence of 60000-100000 mu/ml to be 6.5-10.0 by adopting alkali to obtain alkalic fermentation liquor; adding calcium carbonate with the addition amount of 0 to 25% by weight of the fermentation liquor to obtain salinomycin feed liquor; and spraying and drying the salinomycin feed liquor obtained in step a to obtain the salinomycin particle premix. The method can be used for preparing the salinomycin particle premix with high uniformity and stability by a rapid and energy-saving mode, and the content of the salinomycin particle premix can be up to 31%.
Description
Technical field
The present invention relates to field of veterinary, particularly the method for preparing of salinomycin particle premix.
Background technology
Salinomycin is a monocarboxylic acid polyethers animal specific antibiotic; Most of gram positive bacterias and various coccidiosis there are stronger inhibition and killing action, are difficult for producing drug resistance and cross-resistance, drain rapidly; Residual quantity is extremely low, can be used for preventing and treating diarrhoea, growth promotion, raising survival rate.Salinomycin has the cation transport effect in cell, especially the affinity to potassium ion, sodium ion, rubidium ion is high especially, and these ion selectivities are flowed out outside films, causes ionic equilibrium to destroy, the osmotic pressure imbalance, cell expansion, be out of shape, break, disintegrate.
The method for preparing of Salinomycin pre-mixing agent comprises solvent extraction, spray drying method, plate-and-frame filtration method etc.Application number is that 93115177.5 Chinese patent discloses a kind of method of utilizing the Powdered feed grade Salinomycin Sodium of spray drying method for preparation; The Salinomycin fermentation liquid is heated through alkalization; After separating concentration, make excipient, adopt spray-dired method convection drying Salinomycin mycelium with calcium carbonate; Obtain the feed grade Salinomycin Sodium of different content, its finished product is the fawn uniform powder.This method is made Powdered Salinomycin Sodium, removes in the production process and very easily produces dust, influence the operative employee healthy outside, also progressively be eliminated along with the popularization of pellet.Application number is the method for preparing that 200410023920.1 Chinese patent discloses a kind of Salinomycin Sodium granule; Supernatant concentration is gone in Salinomycin fermentation liquid sedimentation after sterilization treatment; Add adjuvant and medicinal adhesive a-starch, methylol fiber sodium and sodium carboxymethyl cellulose in proportion; The adjustment pH value is an alkalescence, processes the dried granule of Salinomycin Sodium with the bed spray seasoning.Though this method can prepare the graininess Salinomycin Sodium by meeting the market requirement, complex procedures, the production cycle is long, and is all unfavorable to production cost and product quality.In addition, the content of the Salinomycin pre-mixing agent that prior art is prepared is generally 10% or 12%, and high-load Salinomycin pre-mixing agent can not reach requirement usually.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of method for preparing of salinomycin particle premix, this method can reduce the influence of external additive, fast, stable, energy-conservation uniformity height and the high Salinomycin pre-mixing agent of content produced.
For realizing above-mentioned purpose, technical scheme of the present invention is:
The method for preparing of salinomycin particle premix may further comprise the steps:
A prepares alkaline broth: will tire is that the Salinomycin fermentation liquid of 60000～100000u/ml is regulated pH to 6.5～10.0 with alkali, alkaline broth;
B prepares the Salinomycin feed liquid: in step a gained alkaline broth, add the calcium carbonate that is equivalent to alkaline broth quality 0～25%, get the Salinomycin feed liquid;
C spray drying: step b gained Salinomycin feed liquid is processed granule with spray drying method, promptly get salinomycin particle premix.
Further, further comprising the steps of between said step a and the step b: as step a gained alkaline broth to be heated to 40～42 ℃ of insulations 4 hours earlier, to add sodium silicate solution and with acid for adjusting pH to 7～8, again according to the said adding calcium carbonate of step b;
Further, the mass concentration of said sodium silicate solution is 0.5%, and the addition of said sodium silicate solution is equivalent to 1～6% of alkaline broth volume.
Beneficial effect of the present invention is: this method is high with the salinomycin particle premix homogeneity and the stability of quick, energy-conservation mode of production preparation, and its content can be up to 31%; It is low at high-load salinomycin particle fat content that the present invention prepares, and then guaranteed quality of stability.
The specific embodiment
Below will carry out detailed description to the preferred embodiments of the present invention with reference to accompanying drawing.
Embodiment 1 content is the method for preparing of 16.5% salinomycin particle premix
The spawn culture of 1 Salinomycin
Select in the inclined-plane the plentiful single bacterium colony of outward appearance several, under aseptic condition, the bacterium colony spore is put into the 5ml sterilized water and shakes up.Evenly be applied to the method for scoring spore suspension and produce on the spore slant medium and place 30 ℃ of thermostatic chambers to cultivate 8 days, well-grown slant pore bottle is indicated strain number, on following bottle of date, preserve in 2～4 ℃ of refrigerators subsequent use.
2 fermenting and producing
Add 30ml water in every inclined-plane of sub-slant pore, carefully scrape spore and make it not carry any culture medium material, merge and pour in the aseptic bottle, pressure differential method is inoculated in the 500L first class seed pot then, and after 30 hours, mycelia polybrochate merogenesis mycelia is sturdy, does not have assorted bacterium.Abnormal smells from the patient is normal, and light yellow, PH7.0, Biomass are 22%; With its subcultivation to 3m
3In the secondary seed jar, after 10 hours, the growth mycelia is sturdy, and branch is few, and polybrochate does not have assorted bacterium PH7.0, and Biomass is 29%, and subcultivation is to 6m
3In the fermentation tank, at 35 ℃ of fermentation culture 268 hours, ventilation 1.3, pressure 0.05Mpa, moisturizing 800L, repairing 348L, logical alkali 50L; Putting a jar Salinomycin tires: 78000u/ml.
3 feed liquids are handled
Get above-mentioned fermentation liquid 500L, get in the storage tank, pH is transferred to 9.8, add calcium carbonate 120Kg, stir with caustic soda solution.
4 spray dryinges
Open spray drying device, when wind pushing temperature reaches 120 ℃ of left and right sides, open intake valve, close exhaust valve.Temperature is raised to 70 ℃ in tower, when temperature of outgoing air is 60 ℃, starts centrifuge.Open inlet valve then, a small amount of charging is also adjusted the feed liquid flow at any time, and when EAT rises to more than 140 ℃, temperature in the tower, temperature of outgoing air are stable, and atomizing is good, normal charging.Obtain Salinomycin Sodium granule semi-finished product 220Kg.
5. hybrid packed
To be transported to through the semi-finished product that are up to the standards after the spray drying in the mixer, through the mixer uniform mixing after 40 minutes, so that obtain the mixture of the even homogeneity of this product.The mass fraction of in time notifying reviewer's sampling inspection wherein to contain Salinomycin behind the mix homogeneously is 16.5%, and the product that is up to the standards can import packing into from mixer.
Embodiment 2 content are the method for preparing of 31% salinomycin particle premix
1. the spawn culture of Salinomycin
Select in the inclined-plane the plentiful single bacterium colony of outward appearance several, under aseptic condition, the bacterium colony spore is put into the 5ml sterilized water and shakes up.Evenly be applied to the method for scoring spore suspension and produce on the spore slant medium and place 30 ℃ of thermostatic chambers to cultivate 8 days, well-grown slant pore bottle is indicated strain number, on following bottle of date, preserve in 2～4 ℃ of refrigerators subsequent use.
2. fermenting and producing
Add 30ml water in every inclined-plane of sub-slant pore, carefully scrape spore and make it not carry any culture medium material, merge and pour in the aseptic bottle, pressure differential method is inoculated in the 500L first class seed pot then, and after 32 hours, mycelia polybrochate merogenesis mycelia is sturdy, does not have assorted bacterium.Abnormal smells from the patient is normal, and light yellow, PH7.0, Biomass are 24%; With its subcultivation to 3m
3In the secondary seed jar, after 9 hours, the growth mycelia is sturdy, and branch is few, and polybrochate does not have assorted bacterium PH7.0, and Biomass is 32%, and subcultivation is to 6m
3In the fermentation tank, at 33 ℃ of fermentation culture 288 hours, ventilation 1.3, pressure 0.05Mpa, moisturizing 900L, repairing 402L, logical alkali 78L; Putting a jar Salinomycin tires: 72000u/ml.
3 feed liquids are handled
Get above-mentioned 500L fermentation liquid, get in the storage tank, pH is transferred to 9.5 with sodium hydroxide solution; Fermentation liquid is heated to 40 degrees centigrade, is incubated 4 hours, adding mass concentration is 0.5% sodium silicate solution 30L; Transfer PH to 7.5 with hydrochloric acid, add calcium carbonate 20Kg, stir.
4 spray dryinges
Open spray drying device, when wind pushing temperature reaches 120 ℃ of left and right sides, open intake valve, close exhaust valve.Temperature is raised to 70 ℃ in tower, when temperature of outgoing air is 60 ℃, starts centrifuge.Open inlet valve then, a small amount of charging is also adjusted the feed liquid flow at any time, and when EAT rises to more than 140 ℃, temperature in the tower, temperature of outgoing air are stable, and atomizing is good, normal charging.Obtain salinomycin particle semi-finished product 110Kg.
5. hybrid packed
To be transported to through the semi-finished product that are up to the standards after the spray drying in the mixer, through the mixer uniform mixing after 40 minutes, so that obtain the mixture of the even homogeneity of this product.The mass fraction of in time notifying reviewer's sampling inspection wherein to contain Salinomycin behind the mix homogeneously is 31%, and the product that is up to the standards can import packing into from mixer.
In the method for preparing of above-mentioned salinomycin particle premix, the realization of consumption that can be through regulating calcium carbonate prepares the salinomycin particle premix of different content, wherein, when the consumption of calcium carbonate is 0, can prepare the highest salinomycin particle premix of content; When the higher salinomycin particle premix of preparation content; Usually can produce the too high problem of fat content; And then cause salinomycin particle in bulk and the unstable a series of problems of quality in the storage process, and can reach except that greasy purpose through adding sodium silicate solution.
Explanation is at last; Above embodiment is only unrestricted in order to technical scheme of the present invention to be described; Although through invention has been described with reference to the preferred embodiments of the present invention; But those of ordinary skill in the art should be appreciated that and can make various changes to it in form with on the details, and the spirit and scope of the present invention that do not depart from appended claims and limited.
Claims (1)
1. the method for preparing of salinomycin particle premix is characterized in that, following steps are arranged:
A. the spawn culture of Salinomycin
Select in the inclined-plane the plentiful single bacterium colony of outward appearance several; Under aseptic condition, the bacterium colony spore being put into the 5ml sterilized water shakes up; Evenly be applied to the method for scoring spore suspension and produce on the spore slant medium and place 30 ℃ of thermostatic chambers to cultivate 8 days, preserve in 2～4 ℃ of refrigerators well-grown slant pore bottle subsequent use;
B. fermenting and producing
Add 30ml water in every inclined-plane of slant pore, carefully scrape spore and make it not carry any culture medium material, merge and pour in the aseptic bottle; Pressure differential method is inoculated in the 500L first class seed pot then, and after 32 hours, mycelia polybrochate merogenesis mycelia is sturdy; There is not assorted bacterium; Abnormal smells from the patient is normal, and light yellow, pH7.0, Biomass are 24%; With its subcultivation to 3m
3In the secondary seed jar, after 9 hours, the growth mycelia is sturdy, and branch is few, and polybrochate does not have assorted bacterium pH7.0, and Biomass is 32%, and subcultivation is to 6m
3In the fermentation tank, at 33 ℃ of fermentation culture 288 hours, ventilation 1.3, pressure 0.05Mpa, moisturizing 900L, repairing 402L, logical alkali 78L; Putting a jar Salinomycin tires: 72000u/ml;
The C feed liquid is handled
Get above-mentioned 500L fermentation liquid, get in the storage tank, pH is transferred to 9.5 with sodium hydroxide solution; Fermentation liquid is heated to 40 degrees centigrade, is incubated 4 hours, adding mass concentration is 0.5% sodium silicate solution 30L; Transfer pH to 7.5 with hydrochloric acid, add calcium carbonate 20Kg, stir;
The d spray drying
Open spray drying device, when wind pushing temperature reaches 120 ℃, open intake valve, close exhaust valve; Temperature is raised to 70 ℃ in tower, when temperature of outgoing air is 60 ℃, starts centrifuge; Open inlet valve then, a small amount of charging is also adjusted the feed liquid flow at any time, when EAT rises to more than 140 ℃; Temperature, temperature of outgoing air are stablized in the tower, and atomizing is good, normal charging; Obtain salinomycin particle semi-finished product 110Kg;
E. mix
To be transported to through the semi-finished product that are up to the standards after the spray drying in the mixer, through the mixer uniform mixing after 40 minutes, so that obtain the mixture of the even homogeneity of this product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2010102131911A CN101884621B (en) | 2010-06-29 | 2010-06-29 | Method for preparing salinomycin particle premix |
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|---|---|---|---|
| CN2010102131911A CN101884621B (en) | 2010-06-29 | 2010-06-29 | Method for preparing salinomycin particle premix |
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| CN101884621B true CN101884621B (en) | 2012-04-18 |
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| CN108309953B (en) * | 2018-03-30 | 2019-02-26 | 内蒙古拜克生物有限公司 | A kind of production method of salinomycin microcapsule granule |
| CN108420796B (en) * | 2018-03-30 | 2019-02-26 | 内蒙古拜克生物有限公司 | A kind of salinomycin particle premix and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1087791A (en) * | 1993-10-20 | 1994-06-15 | 山东鲁抗医药(集团)股份有限公司 | The preparation method of feed grade salinomycin |
| CN1631365A (en) * | 2004-04-14 | 2005-06-29 | 山东鲁抗舍里乐药业有限公司 | Sodium salinomycin powder and its preparation |
| CN101366461A (en) * | 2008-10-06 | 2009-02-18 | 山东齐发药业有限公司 | Preparation method for salinomycin sodium fine granular formulation |
-
2010
- 2010-06-29 CN CN2010102131911A patent/CN101884621B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1087791A (en) * | 1993-10-20 | 1994-06-15 | 山东鲁抗医药(集团)股份有限公司 | The preparation method of feed grade salinomycin |
| CN1631365A (en) * | 2004-04-14 | 2005-06-29 | 山东鲁抗舍里乐药业有限公司 | Sodium salinomycin powder and its preparation |
| CN101366461A (en) * | 2008-10-06 | 2009-02-18 | 山东齐发药业有限公司 | Preparation method for salinomycin sodium fine granular formulation |
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Denomination of invention: Preparation of salinomycin granule premix Effective date of registration: 20201228 Granted publication date: 20120418 Pledgee: INNER MONGOLIA BRANCH OF BANK OF COMMUNICATIONS Co.,Ltd. Pledgor: JINHE BIO-TECHNOLOGY Co.,Ltd. Registration number: Y2020150000082 |
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