CN104313079B - Preparation method of monensin premix - Google Patents
Preparation method of monensin premix Download PDFInfo
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- CN104313079B CN104313079B CN201410608474.4A CN201410608474A CN104313079B CN 104313079 B CN104313079 B CN 104313079B CN 201410608474 A CN201410608474 A CN 201410608474A CN 104313079 B CN104313079 B CN 104313079B
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Abstract
The invention discloses a preparation method of a monensin premix. The preparation method comprises the following specific steps: preparing cinnamon streptomyce strain; seed flask culture and seed tank culture are performed; when the age of the seed is 20-24h, the pH of a culture solution is 6.6-7.1, the biomass is above 10%, and microscopic examination shows that mycelia are thick and reticular, are stretched and darkly stained and are free of foreign bacteria, performing fermentation culture is performed; when the residual oil amount is below 0.7%, the biomass is above 40%, the mycelium concentration is 40-50%, and the monensin titer is not lower than 40000U/mL, fermentation is finished; monensin is extracted by a one-step spray drying method, wherein the prepared finished monensin product has the monensin content of above 20% and the grain rate of above 75%. According to the preparation method, the fermentation process is simple, so that the fermentation unit can be effectively increased and the production cost can be reduced; usages of an animal-derived nitrogen source and a chemical organic solvent are avoided.
Description
Technical field
The invention belongs to field of veterinary, is related to a kind of preparation method of antibiotic pre-mixing agent, and in particular to monensin is pre-
The preparation method of mixture.
Background technology
Monensin (monensin), belongs to polyether antibioticses, in 1967 first by Haney et al. from Cortex Cinnamomi ground strepto-
It is isolated in the fermentation liquid of bacterium (Streptomyces cinnamonensis).20 century 70s are opened as coccidiostat
Beginning puts on market., respectively at, official approval in 1977 in 1974 as feed additive, China is in 1985 for the U.S., Japan
Year is granted, and is applied to production.The growth promoter that more than 40 countries are used as beef cattle, the weighting agent of meat sheep and pig is had at present.
Monensin but has one to staphylococcuses, bacillus, clostridium, streptococcus, mycete (penicillium, candidiasises) to negative bacterium without effect
Fixed inhibitory action.Monensin can affect ruminant tumor gastric self-energy metabolism, improve rumen zymosiss, improve propanoic acid and second
The yield ratio of acid, reduces volatile fatty acid, reduces methane and generates, improves Protein utilization.Therefore, it is possible to significantly carry
The efficiency of feed utilization of high ruminant and the effect of promotion growth.
With the further development of animal husbandry, in recent years not can the market demand of pre-mixing agent constantly expand, it is and domestic and international
Client to not can pre-mixing agent prescription more and more higher, and there are following shortcomings in existing some preparation methoies：Extraction process
Complexity, chemical solvent consumption are big, and so as to produce substantial amounts of solvent slop and waste residue, production cost and environmentally friendly cost are larger；Product
Granule rate is relatively low, is unfavorable for dispersion of the monensin in feedstuff；During domestic and international feedstuff industry has been used during fermentation culture
The raw material for strictly prohibitting the use of such as fish flour etc., causes production extension realize.In view of this, it is necessary to can not to existing
The preparation method of rhzomorph pre-mixing agent is improved further.
The content of the invention
It is an object of the invention to provide a kind of preparation method of monensin premix, the method is using a step spray dried
It is dry, improve product particle rate.
The concrete technical scheme that the present invention is adopted is as follows：
The preparation method of monensin premix, comprises the following steps：
A. seed culture
The Cortex Cinnamomi prepared through strain ground streptomycete is carried out into seed flask culture and seed tank culture successively；It is described
The condition of culture of seed flask culture is the rotating speed with 220～260rpm, and 24～27h is cultivated under the conditions of 32 ± 0.5 DEG C；It is described
The inoculum concentration of seed tank culture is 0.0035% seed liquor；The condition of culture of the seed tank culture be temperature be 32 ± 0.5
DEG C, tank pressure is 20～24h of culture under the conditions of 0.02～0.05MPa；
Culture medium needed for the seed flask culture is consisted of the following composition based on mass percentage concentration：Soybean cake powder 1
～1.5%, yeast powder 0.1～0.3%, glucose 0.2～0.5%, dextrin 1.0～2.0%, Calcium Carbonate 0.05～0.1% are remaining
Measure as water；After medium sterilization, pH value is 6.9～7.1；
The culture medium of the seed tank culture is consisted of the following composition based on mass percentage concentration：Soybean cake powder 1.0～
1.5%, yeast powder 0.1～0.25%, glucose 1.5～2.5%, Calcium Carbonate 0.1～0.25%, defoamer 0.05～0.1%,
Balance of water；After medium sterilization, pH value is 6.6～7.1；
B. fermentation culture
The culture fluid containing strain after step a is cultivated is proceeded to
For：34 ± 1 DEG C of temperature in tank, dissolved oxygen amount more than 30%, 0.03～0.05MPa of tank pressure；It is molten by adding ammonia in sweat
Liquid, vegetable oil and sterilized water, so that fermentation liquid pH value is maintained between 6.5～6.6, vegetable oil is not low based on mass percentage concentration
In 3%, dissolved oxygen amount is not less than 30%, and sweat terminates to stop plus vegetable oil for first 24～48 hours；When fermentation liquid pH value is up to 6.6
～7.0, resid amount is less than 0.7%, and Biomass more than 40%, when monensin potency is not less than 40000U/mL, tie by fermentation
Beam；
Fermentation medium is consisted of the following composition based on mass percentage concentration：Glucose 2.0～3.8%, soybean cake powder 1.8
～3.7%, vegetable oil 1.9～2.5%, methyl oleate 0.05～1.0%, Calcium Carbonate 0.15～0.35%, sodium sulfate 0.15～
0.22%, sodium nitrate 0.15～0.22%, manganese chloride 0.01～0.03%, ferrous sulfate 0.005～0.01%, ascorbic acid
0.001～0.002%, aluminum sulfate 0.05～0.07%, dipotassium hydrogen phosphate 0.005～0.01%, defoamer 0.01～0.02%,
Balance of water；
C. extract
After fermentation ends, fermentation liquid pH value is adjusted to into 10～11 first, is then again heated to 63 DEG C and is incubated 3 hours, respectively
After adding anhydrous sodium metasilicate and Calcium Carbonate, fermentation liquid pH value is adjusted to into 7～8 and is placed 30 minutes, be finally spray-dried；The nothing
Water sodium silicate addition for fermentation liquid weight 6%, the Calcium Carbonate addition for fermentation liquid weight 0～35%；The spray
Mist be dried condition be：140～160 DEG C of inlet temperature；65～95 DEG C of indoor temperature is dried, 60～70 DEG C of temperature of outgoing air is dried
- 280～-380MPa of pressure in device.
Preferably, the culture medium described in step a needed for seed flask culture based on mass percentage concentration by following
Into being grouped into：Soybean cake powder 1.5%, yeast powder 0.2%, glucose 0.4%, dextrin 1.0%, Calcium Carbonate 0.06% are balance of
Water.
Preferably, the culture medium of seed tank culture described in step a based on mass percentage concentration by following component group
Into：Soybean cake powder 1.5%, yeast powder 0.2%, glucose 2%, Calcium Carbonate 0.25%, defoamer 0.1%, balance of water.
Preferably, described in step a, fermentation medium is consisted of the following composition based on mass percentage concentration：Glucose
3%, soybean cake powder 3%, vegetable oil 2%, methyl oleate 1.0%, Calcium Carbonate 0.2%, sodium sulfate 0.2%, sodium nitrate 0.2%,
Manganese chloride 0.02%, ferrous sulfate 0.01%, ascorbic acid 0.001%, aluminum sulfate 0.06%, dipotassium hydrogen phosphate 0.01% disappear
Infusion 0.01%, balance of water.
Preferably, step a is that the strain that will be prepared through strain carries out seed flask culture and seed successively
Tank culture；The condition of culture of the seed flask culture is the rotating speed with 220～260rpm, is cultivated under the conditions of 32 ± 0.5 DEG C
25h；The inoculum concentration of the seed tank culture is 0.0035% seed liquor；The condition of culture of the seed tank culture is to be in temperature
32 ± 0.5 DEG C, tank pressure be 0.04MPa under the conditions of cultivate 24h.
Preferably, the culture fluid containing strain after step b is by the culture of step a is proceeded in fermentation tank and is fermented
Cultivate, fermentation culture conditions are：In tank, temperature is 34 ± 1 DEG C, and, more than 30%, tank pressure is in 0.03MPa, sweat for dissolved oxygen amount
In by adding ammonia spirit, vegetable oil and sterilized water so that fermentation liquid pH value is maintained between 6.5～6.6, vegetable oil presses matter
Amount percentage concentration meter is not less than 3%, and dissolved oxygen amount is not less than 30%, and sweat terminates to stop plus vegetable oil for first 24～48 hours；
When fermentation liquid pH is 6.9, resid amount is 0.6%, and Biomass 45%, when monensin potency is 45690u/mL, tie by fermentation
Beam.
Preferably, step c is then heated to 63 DEG C and is incubated 3 hours for fermentation liquid pH value is adjusted to 10.8 first, plus
Fermentation liquid pH value is adjusted to into 7 after entering anhydrous sodium metasilicate and Calcium Carbonate and is placed 30 minutes, be finally spray-dried；The anhydrous silicic acid
Sodium addition for fermentation liquid weight 6%, the Calcium Carbonate addition for fermentation liquid weight 20%；The bar of the spray drying
Part is：155 DEG C of inlet temperature；It is dried 87 DEG C of indoor temperature, 67 DEG C of temperature of outgoing air, pressure -300MPa in exsiccator.
In seed flask incubation, when medium pH value is 6.6～6.7, Biomass is 6.9～15%, and color is
Canescence, and microscopy result shows that mycelial growth is good, with many branches into threadiness, without miscellaneous bacteria when, switch to seed tank training
Support.
During seed tank culture, when culture to seed age is 20～24h, medium pH value is 6.6～7.1, biological
Measure as more than 10%, microscopy result show mycelia it is sturdy in it is netted, unfold, dye depth, without miscellaneous bacteria when, switch to fermentation culture.
The beneficial effects of the present invention is：Fermentation technology of the present invention is simple, can effectively improve fermentation unit, and reduction is produced into
This, while avoiding the use of animal derived nitrogen source and chemical organic solvent, prevents organic solvent to monensin premix
Pollute, it is to avoid harm to animal；And the conjunction of monensin special construction is beneficial to using culture medium of the present invention
Into so as to improve monensin yield, moreover it is possible to be conducive to the follow-up extraction to monensin premix；In addition, being sprayed using a step
Mist is dried, and will not produce sewage, reduce environmental pollution；Obtained monensin finished product content is more than 20%, granule rate
Up to more than 75%, product particle rate is significantly improved, realize that monensin stability and high efficiency is produced.
Specific embodiment
Below the preferred embodiments of the present invention are described in detail.The experiment side of unreceipted actual conditions in embodiment
Method, generally according to normal condition or according to the condition proposed by manufacturer.
Embodiment 1
The preparation method of monensin premix is as follows：
A. seed culture
1) prepared by strain
The sand pipe containing meat ground osmanthus streptomycete is taken, is added 5mL sterilized water to shake up under aseptic condition, is then used aseptic inoculation pin
It is applied in product spore slant medium, is placed in 32 DEG C ± 0.5 DEG C and cultivates 11 days.
2) seed flask culture
By step 1) spore inoculating for preparing in the culture medium of shake-flask culture, with the rotating speed of 220-260rpm, 32 ±
Constant temperature culture 25 hours at 0.5 DEG C.
The culture medium of shake-flask culture is made up of following component based on mass percentage concentration：Soybean cake powder 1.5%, yeast powder
0.2%, glucose 0.4%, dextrin 1.0%, Calcium Carbonate 0.06%, balance of water；Sterilizing wild Oryza species pH value is 7.05.
3) seed tank culture
By step 2) culture after strain add seed tank in, inoculum concentration be 0.0035% seed liquor, temperature be 32
± 0.5 DEG C, for 24h is cultivated under the conditions of 0.04MPa, sampling is detected tank pressure.
Testing result：Outward appearance is in light yellow, thick, and abnormal smells from the patient is normal, and without miscellaneous bacteria, pH value is 6.7, and Biomass is 18%, Mo Neng
Rhzomorph potency be 160U/mL, mycelia it is sturdy in it is netted, unfold, dye depth, reach seed culture fluid quality control index.
Used medium is consisted of the following composition based on mass percentage concentration：Soybean cake powder 1.5%, yeast powder 0.2%, Portugal
Grape sugar 2%, Calcium Carbonate 0.25%, defoamer 0.1%, balance of water；After sterilizing, pH value is 6.7.
B. fermentation culture
By the culture fluid subcultivation of seed culture to fermentation tank, in tank, temperature maintains 34 ± 1 DEG C, dissolved oxygen amount 30% with
On, tank pressure is in 0.03MPa.By adding ammonia spirit, vegetable oil and sterilized water in sweat, so that fermentation liquid pH value is maintained
Between 6.5～6.6, vegetable oil is not less than 3% based on mass percentage concentration, and dissolved oxygen amount is not less than 30%, and sweat terminates
Stop plus vegetable oil within first 24～48 hours；Ferment to 290 hours and terminate, sampling detection.
Testing result：PH value is 6.9, and Biomass is 45%, and monensin potency is 45690U/mL, and Residual oil is 0.6%,
Now mycelia starts disconnected section, partial autolysis.
The culture medium of the fermentation culture is calculated as by mass percentage concentration：Glucose 3%, soybean cake powder 3%, vegetable oil
2%, methyl oleate 1.0%, Calcium Carbonate 0.2%, sodium sulfate 0.2%, sodium nitrate 0.2%, manganese chloride 0.02%, ferrous sulfate
0.01%, ascorbic acid 0.001%, aluminum sulfate 0.06%, dipotassium hydrogen phosphate 0.01%, defoamer 0.01%, balance of water.Training
Foster base NaOH adjusts pH value to 8.2, standby after constant volume sterilizing.
C. extract
After fermentation ends, fermentation liquid pH value is adjusted to into 10.8 with NaOH first, is then heated to 63 DEG C and is incubated 3 hours, then plus
Enter anhydrous sodium metasilicate, anhydrous sodium metasilicate addition is the 6% of fermentation liquid weight, is subsequently adding Calcium Carbonate, and addition is fermentation liquid
Fermentation liquid pH value is adjusted to 7 and is placed half an hour with sulphuric acid or hydrochloric acid, is finally spray-dried by the 20% of weight afterwards.Spray dried
Dry condition is：155 DEG C of inlet temperature；It is dried 87 DEG C of indoor temperature, 67 DEG C of temperature of outgoing air, in exsiccator, pressure is -300MPa.Most
Monensin finished product content is 34% afterwards, granule rate 87%.
Embodiment 2
The preparation method of monensin premix is as follows：
A. seed culture
1) strain is prepared with embodiment 1.
2) seed flask culture
Operation is with embodiment 1.
The culture medium of shake-flask culture is made up of following ingredients based on mass percentage concentration：Soybean cake powder 1.4%, yeast powder
0.2%, glucose 0.35%, dextrin 1.5%, Calcium Carbonate 0.08%, balance of water.After medium sterilization, pH value is 7.0.
3) seed tank culture
By step 2) culture after strain add seed tank in, inoculum concentration be 0.0035% seed liquor, temperature be 32
± 0.5 DEG C, for 23h is cultivated under the conditions of 0.04MPa, sampling is detected tank pressure.
Testing result：Outward appearance is in light yellow, thick, and abnormal smells from the patient is normal, and without miscellaneous bacteria, pH value is 6.9, and Biomass is 20%, Mo Neng
Rhzomorph potency be 178U/mL, mycelia it is sturdy in it is netted, unfold, dye depth, reach seed culture fluid quality control index.
Used medium is made up of following ingredients based on mass percentage concentration：Soybean cake powder 1.0%, yeast powder 0.15%,
Glucose 2.1%, Calcium Carbonate 0.2%, defoamer 0.1%, balance of water.Sterilizing wild Oryza species pH value is 6.7.
B. fermentation culture
By step 3), to fermentation tank, in tank, temperature maintains 34 ± 1 DEG C for the strain subcultivation cultivated, dissolved oxygen amount 30% with
On.By adding ammonia spirit, vegetable oil and sterilized water in sweat so that fermentation liquid pH value maintain 6.5～6.6 it
Between, vegetable oil is not less than 3% based on mass percentage concentration, and dissolved oxygen amount is not less than 30%, and sweat terminates first 24～48 hours
Stop plus vegetable oil；Ferment to 275 hours and terminate, sampling detection.
Testing result：PH value is 6.8, and Biomass is 50%, and monensin potency is 51430u/mL, and Residual oil is 0.7%,
Now mycelia starts disconnected section, partial autolysis.
The culture medium of the fermentation culture is calculated as by mass percentage concentration：Glucose 3%, soybean cake powder 2.2%, vegetable oil
2.2%, methyl oleate 1.0%, Calcium Carbonate 0.2%, sodium sulfate 0.2%, sodium nitrate 0.2%, manganese chloride 0.01%, ferrous sulfate
0.01%, ascorbic acid 0.001%, aluminum sulfate 0.05%, dipotassium hydrogen phosphate 0.01%, defoamer 0.01%, balance of water.Training
Foster base NaOH adjusts pH value to 8, standby after constant volume sterilizing.
C. extract
Fermentation liquid pH value is adjusted to into 10.5 first, 63 DEG C is then heated to and is incubated 3 hours, add anhydrous sodium metasilicate, add
6% for fermentation liquid weight is measured, Calcium Carbonate is added afterwards, its addition for fermentation liquid weight 15%.Again by the pH of fermentation liquid
Value is adjusted to 7.2 and places half an hour, is finally spray-dried.Spray drying condition is：160 DEG C of inlet temperature；It is dried indoor temperature
90 DEG C, 72 DEG C of temperature of outgoing air, in exsiccator, pressure is -310 MPa.Last monensin finished product content is 30%, granule rate
80%.
It should be noted that the addition of Calcium Carbonate is relevant with tank potency is put in extraction process, monensin effect when putting tank
Valency is high, then the increase of Calcium Carbonate addition.
Finally illustrate, preferred embodiment above is only unrestricted to illustrate technical scheme, although logical
Cross above preferred embodiment to be described in detail the present invention, it is to be understood by those skilled in the art that can be
Various changes are made to which in form and in details, without departing from claims of the present invention limited range.
Claims (7)
1. the preparation method of monensin premix, it is characterised in that comprise the following steps：
A. seed culture
The Cortex Cinnamomi prepared through strain ground streptomycete is carried out into seed flask culture and seed tank culture successively；The seed
The condition of culture of shake-flask culture is the rotating speed with 220～260rpm, and 24～27h is cultivated under the conditions of 32 ± 0.5 DEG C；The seed
The inoculum concentration of tank culture is 0.0035% seed liquor；The condition of culture of the seed tank culture be temperature be 32 ± 0.5 DEG C, tank
Press as 20～24h is cultivated under the conditions of 0.02～0.05MPa；
Culture medium needed for the seed flask culture is consisted of the following composition based on mass percentage concentration：Soybean cake powder 1～
1.5%, yeast powder 0.1～0.3%, glucose 0.2～0.5%, dextrin 1.0～2.0%, Calcium Carbonate 0.05～0.1%, surplus
For water；After medium sterilization, pH value is 6.9～7.1；
The culture medium of the seed tank culture is consisted of the following composition based on mass percentage concentration：Soybean cake powder 1.0～1.5%,
Yeast powder 0.1～0.25%, glucose 1.5～2.5%, Calcium Carbonate 0.1～0.25%, defoamer 0.05～0.1% are balance of
Water；After medium sterilization, pH value is 6.6～7.1；
B. fermentation culture
The culture fluid containing strain after step a is cultivated is proceeded to, and fermentation culture conditions are：Tank
34 ± 1 DEG C of interior temperature, dissolved oxygen amount more than 30%, 0.03～0.05MPa of tank pressure；By adding ammonia spirit, planting in sweat
Thing oil and sterilized water, so that fermentation liquid pH value is maintained between 6.5～6.6, vegetable oil is not less than based on mass percentage concentration
3%, dissolved oxygen amount is not less than 30%, and sweat terminates to stop plus vegetable oil for first 24～48 hours；When fermentation liquid pH value up to 6.6～
7.0, resid amount is less than 0.7%, and Biomass more than 40%, when monensin potency is not less than 40000U/mL, tie by fermentation
Beam；
Fermentation medium is consisted of the following composition based on mass percentage concentration：Glucose 2.0～3.8%, soybean cake powder 1.8～
3.7%, vegetable oil 1.9～2.5%, methyl oleate 0.05～1.0%, Calcium Carbonate 0.15～0.35%, sodium sulfate 0.15～
0.22%, sodium nitrate 0.15～0.22%, manganese chloride 0.01～0.03%, ferrous sulfate 0.005～0.01%, ascorbic acid
0.001～0.002%, aluminum sulfate 0.05～0.07%, dipotassium hydrogen phosphate 0.005～0.01%, defoamer 0.01～0.02%,
Balance of water；
C. extract
After fermentation ends, fermentation liquid pH value is adjusted to into 10～11 first, is then again heated to 63 DEG C and is incubated 3 hours, be separately added into
After anhydrous sodium metasilicate and Calcium Carbonate, fermentation liquid pH value is adjusted to into 7～8 and is placed 30 minutes, be finally spray-dried；The anhydrous silicon
Sour sodium addition for fermentation liquid weight 6%, the Calcium Carbonate addition for fermentation liquid weight 0～35%；The spray dried
Dry condition is：140～160 DEG C of inlet temperature；It is dried 65～95 DEG C of indoor temperature, 60～70 DEG C of temperature of outgoing air, in exsiccator
- 280～-380MPa of pressure.
2. the preparation method of monensin premix according to claim 1, it is characterised in that plant described in step a
Culture medium needed for sub- shake-flask culture is consisted of the following composition based on mass percentage concentration：Soybean cake powder 1.5%, yeast powder
0.2%, glucose 0.4%, dextrin 1.0%, Calcium Carbonate 0.06%, balance of water.
3. the preparation method of monensin premix according to claim 1, it is characterised in that plant described in step a
The culture medium of sub- tank culture is consisted of the following composition based on mass percentage concentration：Soybean cake powder 1.5%, yeast powder 0.2%, Fructus Vitis viniferae
Sugar 2%, Calcium Carbonate 0.25%, defoamer 0.1%, balance of water.
4. the preparation method of monensin premix according to claim 1, it is characterised in that send out described in step a
Ferment culture medium is consisted of the following composition based on mass percentage concentration：Glucose 3%, soybean cake powder 3%, vegetable oil 2%, Oleic acid first
Ester 1.0%, Calcium Carbonate 0.2%, sodium sulfate 0.2%, sodium nitrate 0.2%, manganese chloride 0.02%, ferrous sulfate 0.01% are anti-bad
Hematic acid 0.001%, aluminum sulfate 0.06%, dipotassium hydrogen phosphate 0.01%, defoamer 0.01%, balance of water.
5. the preparation method of monensin premix according to claim 1, it is characterised in that step a is to pass through
The strain that strain is prepared carries out seed flask culture and seed tank culture successively；The condition of culture of the seed flask culture
Be the rotating speed with 220～260rpm, 25h is cultivated under the conditions of 32 ± 0.5 DEG C；The inoculum concentration of the seed tank culture is
0.0035% seed liquor；The condition of culture of the seed tank culture be temperature be 32 ± 0.5 DEG C, tank pressure be 0.04MPa conditions
Lower culture 24h.
6. the preparation method of monensin premix according to claim 1, it is characterised in that step b is by step a
The culture fluid containing strain after culture is proceeded to, and fermentation culture conditions are：In tank, temperature is 34
± 1 DEG C, more than 30%, tank pressure is in 0.03MPa, sweat by adding ammonia spirit, vegetable oil and aseptic for dissolved oxygen amount
Water, so that fermentation liquid pH value is maintained between 6.5～6.6, vegetable oil is not less than 3% based on mass percentage concentration, and dissolved oxygen amount is not
Less than 30%, sweat terminates to stop plus vegetable oil for first 24～48 hours；When fermentation liquid pH is 6.9, resid amount is 0.6%,
Biomass is in 45%, when monensin potency is 45690u/mL, fermentation ends.
7. according to any one of claim 1～6 monensin premix preparation method, it is characterised in that step c
For fermentation liquid pH value is adjusted to 10.8 first, it is then heated to 63 DEG C and is incubated 3 hours, will after adding anhydrous sodium metasilicate and Calcium Carbonate
Fermentation liquid pH value is adjusted to 7 and places 30 minutes, is finally spray-dried；The anhydrous sodium metasilicate addition is fermentation liquid weight
6%, the Calcium Carbonate addition for fermentation liquid weight 20%；The condition of the spray drying is：155 DEG C of inlet temperature；It is dry
87 DEG C of dry indoor temperature, 67 DEG C of temperature of outgoing air, pressure -300MPa in exsiccator.
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| CN110592159B (en) * | 2019-09-23 | 2021-04-09 | 浙江拜克生物科技有限公司 | Preparation process and device of monensin premix |
| CN112410386B (en) * | 2020-10-30 | 2021-08-31 | 内蒙古中牧生物药业有限公司 | Process for improving content of monensin A component |
| CN112680489A (en) * | 2021-01-15 | 2021-04-20 | 驻马店华中正大有限公司 | Method for improving monensin bioavailability |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899377A (en) * | 2012-10-31 | 2013-01-30 | 金河生物科技股份有限公司 | Preparation method of chlortetracycline premix |
| CN103937848A (en) * | 2014-04-14 | 2014-07-23 | 宁夏泰瑞制药股份有限公司 | Novel culture medium for producing monensin by fermenting streptomyces cinnamonensis and culture method |
-
2014
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899377A (en) * | 2012-10-31 | 2013-01-30 | 金河生物科技股份有限公司 | Preparation method of chlortetracycline premix |
| CN103937848A (en) * | 2014-04-14 | 2014-07-23 | 宁夏泰瑞制药股份有限公司 | Novel culture medium for producing monensin by fermenting streptomyces cinnamonensis and culture method |
Non-Patent Citations (1)
| Title |
|---|
| Crotonyl-coenzyme A reductase provides methylmalonyl-CoA precursors for monensin biosynthesis by Streptomyces cinnamonensis in an oil-based extended fermentation;Chaoxuan Li et al.;《Microbiology》;20041231;第150卷;3463-3472 * |
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