CN104313079A - Preparation method of monensin premix - Google Patents
Preparation method of monensin premix Download PDFInfo
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Abstract
The invention discloses a preparation method of a monensin premix. The preparation method comprises the following specific steps: preparing cinnamon streptomyce strain; seed flask culture and seed tank culture are performed; when the age of the seed is 20-24h, the pH of a culture solution is 6.6-7.1, the biomass is above 10%, and microscopic examination shows that mycelia are thick and reticular, are stretched and darkly stained and are free of foreign bacteria, performing fermentation culture is performed; when the residual oil amount is below 0.7%, the biomass is above 40%, the mycelium concentration is 40-50%, and the monensin titer is not lower than 40000U/mL, fermentation is finished; monensin is extracted by a one-step spray drying method, wherein the prepared finished monensin product has the monensin content of above 20% and the grain rate of above 75%. According to the preparation method, the fermentation process is simple, so that the fermentation unit can be effectively increased and the production cost can be reduced; usages of an animal-derived nitrogen source and a chemical organic solvent are avoided.
Description
Technical field
The invention belongs to field of veterinary, relate to a kind of preparation method of microbiotic pre-mixture, be specifically related to the preparation method of monensin premix.
Background technology
Monensin (monensin), belongs to polyether antibiotics, is first separated from the fermented liquid of Chinese cassia tree ground streptomycete (Streptomyces cinnamonensis) by people such as Haney obtains in 1967.20 century 70s start to put on market as anticoccidial drug.The U.S., Japan it can be used as fodder additives respectively at official approval in 1974,1977, and China is granted in 1985, and is applied to production.Existing more than 40 countries are used as beef cattle, the weighting agent of mutton sheep and the growth stimulant of pig at present.Monensin without effect, but has certain restraining effect to staphylococcus, bacillus, clostridium, suis, mould (Penicillium notatum, candidiasis) to negative bacterium.Monensin can affect the metabolism of ruminant tumor gastric self-energy, improves rumen zymosis, improves the yield ratio of propionic acid and acetic acid, reduces voltaile fatty acid, reduces methane generation, improves Protein utilization.Therefore, it is possible to significantly improve the efficiency of feed utilization of ruminating animal and the effect of growth promoting effects.
Along with further developing of livestock industry, can the market demand of pre-mixture constantly not expand in recent years, and both at home and abroad client to not can the specification of quality of pre-mixture more and more higher, and there is following shortcoming in more existing preparation methods: extraction process is complicated, chemical solvents consumption is large, thus produce a large amount of solvent slops and waste residue, production cost and environmental protection cost larger; Product particle rate is lower, is unfavorable for the dispersion of monensin in feed; Employ the raw material that strictly prohibits the use in domestic and international feedstuff industry in fermentation culture process as fish meal etc., cause production extension to realize.In view of this, be necessary to be improved further the preparation method of existing monensin premix.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of monensin premix, the method adopts an one step spray-drying, improves product particle rate.
The concrete technical scheme that the present invention adopts is as follows:
The preparation method of monensin premix, comprises the following steps:
A. seed culture
The Chinese cassia tree prepared through bacterial classification ground streptomycete is carried out seed flask cultivation and seed tank culture successively; The culture condition that described seed flask is cultivated is the rotating speed with 220 ~ 260rpm, under 32 ± 0.5 DEG C of conditions, cultivate 24 ~ 27h; The inoculum size of described seed tank culture is 0.0035% seed liquor; The culture condition of described seed tank culture is for being 32 ± 0.5 DEG C in temperature, and tank pressure is cultivate 20 ~ 24h under 0.02 ~ 0.05MPa condition;
Substratum needed for described seed flask cultivation consists of the following composition by mass percentage concentration: soybean cake powder 1 ~ 1.5%, yeast powder 0.1 ~ 0.3%, glucose 0.2 ~ 0.5%, dextrin 1.0 ~ 2.0%, calcium carbonate 0.05 ~ 0.1%, and surplus is water; After medium sterilization, pH value is 6.9 ~ 7.1;
The substratum of described seed tank culture consists of the following composition by mass percentage concentration: soybean cake powder 1.0 ~ 1.5%, yeast powder 0.1 ~ 0.25%, glucose 1.5 ~ 2.5%, calcium carbonate 0.1 ~ 0.25%, defoamer 0.05 ~ 0.1%, and surplus is water; After medium sterilization, pH value is 6.6 ~ 7.1;
B. fermentation culture
The nutrient solution containing bacterial classification after being cultivated by step a proceeds in fermentor tank and carries out fermentation culture, and fermentation culture conditions is: temperature 34 ± 1 DEG C in tank, dissolved oxygen amount more than 30%, tank pressure 0.03 ~ 0.05MPa; By adding ammonia soln, vegetables oil and sterilized water in fermenting process, maintain between 6.5 ~ 6.6 to make fermented liquid pH value, vegetables oil is not less than 3% by mass percentage concentration, and dissolved oxygen amount is not less than 30%, and fermenting process terminates within first 24 ~ 48 hours, to stop adding vegetables oil; When fermented liquid pH value reaches 6.6 ~ 7.0, resid amount is less than 0.7%, biomass more than 40%, when monensin is tired and is not less than 40000U/mL, fermentation ends;
Fermention medium consists of the following composition by mass percentage concentration: glucose 2.0 ~ 3.8%, soybean cake powder 1.8 ~ 3.7%, vegetables oil 1.9 ~ 2.5%, Witconol 2301 0.05 ~ 1.0%, calcium carbonate 0.15 ~ 0.35%, sodium sulfate 0.15 ~ 0.22%, SODIUMNITRATE 0.15 ~ 0.22%, Manganous chloride tetrahydrate 0.01 ~ 0.03%, ferrous sulfate 0.005 ~ 0.01%, xitix 0.001 ~ 0.002%, Tai-Ace S 150 0.05 ~ 0.07%, dipotassium hydrogen phosphate 0.005 ~ 0.01%, defoamer 0.01 ~ 0.02%, surplus is water;
C. extract
After fermentation ends, first fermented liquid pH value is adjusted to 10 ~ 11, and then is heated to 63 DEG C and is incubated 3 hours, after adding anhydrous sodium metasilicate and calcium carbonate respectively, fermented liquid pH value is adjusted to 7 ~ 8 and places 30 minutes, last spraying dry; Described anhydrous sodium metasilicate add-on is 6% of fermented liquid weight, and described calcium carbonate add-on is 0 ~ 35% of fermented liquid weight; Described spray-dired condition is: inlet temperature 140 ~ 160 DEG C; Dry room temp 65 ~ 95 DEG C, temperature of outgoing air 60 ~ 70 DEG C, moisture eliminator internal pressure-280 ~-380MPa.
Preferably, the substratum needed for the cultivation of seed flask described in described step a consists of the following composition by mass percentage concentration: soybean cake powder 1.5%, yeast powder 0.2%, glucose 0.4%, dextrin 1.0%, calcium carbonate 0.06%, surplus is water.
Preferably, described in described step a, the substratum of seed tank culture consists of the following composition by mass percentage concentration: soybean cake powder 1.5%, yeast powder 0.2%, glucose 2%, calcium carbonate 0.25%, defoamer 0.1%, and surplus is water.
Preferably, described in described step a, fermention medium consists of the following composition by mass percentage concentration: glucose 3%, soybean cake powder 3%, vegetables oil 2%, Witconol 2301 1.0%, calcium carbonate 0.2%, sodium sulfate 0.2%, SODIUMNITRATE 0.2%, Manganous chloride tetrahydrate 0.02%, ferrous sulfate 0.01%, xitix 0.001%, Tai-Ace S 150 0.06%, dipotassium hydrogen phosphate 0.01%, defoamer 0.01%, surplus is water.
Preferably, the bacterial classification prepared through bacterial classification is carried out seed flask cultivation and seed tank culture by described step a successively; The culture condition that described seed flask is cultivated is the rotating speed with 220 ~ 260rpm, under 32 ± 0.5 DEG C of conditions, cultivate 25h; The inoculum size of described seed tank culture is 0.0035% seed liquor; The culture condition of described seed tank culture is for being 32 ± 0.5 DEG C in temperature, and tank pressure is cultivate 24h under 0.04MPa condition.
Preferably, described step b be step a is cultivated after proceed in fermentor tank containing the nutrient solution of bacterial classification and carry out fermentation culture, fermentation culture conditions is: in tank, temperature is 34 ± 1 DEG C, dissolved oxygen amount is more than 30%, tank pressure is at 0.03MPa, by adding ammonia soln, vegetables oil and sterilized water in fermenting process, maintain between 6.5 ~ 6.6 to make fermented liquid pH value, vegetables oil is not less than 3% by mass percentage concentration, dissolved oxygen amount is not less than 30%, and fermenting process terminates within first 24 ~ 48 hours, to stop adding vegetables oil; When fermented liquid pH is 6.9, resid amount is 0.6%, biomass 45%, when monensin is tired as 45690u/mL, fermentation ends.
Preferably, described step c, for first fermented liquid pH value is adjusted to 10.8, is then heated to 63 DEG C and is incubated 3 hours, after adding anhydrous sodium metasilicate and calcium carbonate, fermented liquid pH value is adjusted to 7 and places 30 minutes, last spraying dry; Described anhydrous sodium metasilicate add-on is 6% of fermented liquid weight, and described calcium carbonate add-on is 20% of fermented liquid weight; Described spray-dired condition is: inlet temperature 155 DEG C; Dry room temp 87 DEG C, temperature of outgoing air 67 DEG C, moisture eliminator internal pressure-300MPa.
In seed flask culturing process, when medium pH value is 6.6 ~ 6.7, biomass is 6.9 ~ 15%, and color is canescence, and microscopy result display mycelial growth is good, becomes threadiness with many branches, without miscellaneous bacteria time, transfer seed tank culture to.
In seed tank culture process, be 20 ~ 24h when being cultured to seed age, medium pH value is 6.6 ~ 7.1, and biomass is more than 10%, microscopy result display mycelia sturdy in netted, unfold, dye deeply, without miscellaneous bacteria time, transfer fermentation culture to.
Beneficial effect of the present invention is: zymotechnique of the present invention is simple, effectively can improve fermentation unit, reduce production cost, avoid the use of animal derived nitrogenous source and chemical organic solvent simultaneously, prevent organic solvent from polluting monensin premix, avoid the harm to animal; And adopt substratum of the present invention to be beneficial to the synthesis of monensin special construction, thus improve monensin yield, the follow-up extraction to monensin premix can also be conducive to; In addition, adopt an one step spray-drying, can not sewage be produced, reduce environmental pollution; Obtained monensin finished product content is more than 20%, and particle rate reaches more than 75%, significantly improves product particle rate, realizes monensin stability and high efficiency and produces.
Embodiment
Below the preferred embodiments of the present invention are described in detail.The experimental technique of unreceipted actual conditions in embodiment, the usually conveniently conditioned disjunction condition of advising according to manufacturer.
Embodiment 1
The preparation method of monensin premix is as follows:
A. seed culture
1) bacterial classification preparation
Get the sand pipe containing osmanthus, meat ground streptomycete, add 5mL sterilized water under aseptic condition and shake up, be then applied to aseptic inoculation pin and produce in spore slant medium, and be placed in 32 DEG C ± 0.5 DEG C cultivation 11 days.
2) seed flask is cultivated
By step 1) spore inoculating prepared in the substratum of shake-flask culture, with the rotating speed of 220-260rpm, constant temperature culture 25 hours at 32 ± 0.5 DEG C.
The substratum of shake-flask culture is made up of following component by mass percentage concentration: soybean cake powder 1.5%, yeast powder 0.2%, glucose 0.4%, dextrin 1.0%, calcium carbonate 0.06%, and surplus is water; Sterilizing wild Oryza species pH value is 7.05.
3) seed tank culture
By step 2) cultivate after bacterial classification add in seeding tank, inoculum size is 0.0035% seed liquor, is 32 ± 0.5 DEG C in temperature, and tank pressure is cultivate 24h under 0.04MPa condition, sampling detect.
Detected result: outward appearance is light yellow, thick, smell is normal, and without miscellaneous bacteria, pH value is 6.7, and biomass is 18%, and monensin is tired as 160U/mL, mycelia sturdy in netted, unfold, dye deeply, reach seed culture fluid quality control index.
Used medium consists of the following composition by mass percentage concentration: soybean cake powder 1.5%, yeast powder 0.2%, glucose 2%, calcium carbonate 0.25%, defoamer 0.1%, and surplus is water; After sterilizing, pH value is 6.7.
B. fermentation culture
By the nutrient solution culture transferring of seed culture in fermentor tank, in tank, temperature maintains 34 ± 1 DEG C, and dissolved oxygen amount is more than 30%, and tank pressure is at 0.03MPa.By adding ammonia soln, vegetables oil and sterilized water in fermenting process, maintain between 6.5 ~ 6.6 to make fermented liquid pH value, vegetables oil is not less than 3% by mass percentage concentration, and dissolved oxygen amount is not less than 30%, and fermenting process terminates within first 24 ~ 48 hours, to stop adding vegetables oil; Ferment to 290 hours and terminate, sampling detects.
Detected result: pH value is 6.9, biomass is 45%, and monensin is tired as 45690U/mL, and Residual oil is 0.6%, and now mycelia starts disconnected joint, partial autolysis.
The substratum of described fermentation culture is counted by mass percentage concentration: glucose 3%, soybean cake powder 3%, vegetables oil 2%, Witconol 2301 1.0%, calcium carbonate 0.2%, sodium sulfate 0.2%, SODIUMNITRATE 0.2%, Manganous chloride tetrahydrate 0.02%, ferrous sulfate 0.01%, xitix 0.001%, Tai-Ace S 150 0.06%, dipotassium hydrogen phosphate 0.01%, defoamer 0.01%, surplus is water.Substratum NaOH adjust pH to 8.2, for subsequent use after constant volume sterilizing.
C. extract
After fermentation ends, first with NaOH, fermented liquid pH value is adjusted to 10.8, then 63 DEG C of insulations 3 hours are heated to, add anhydrous sodium metasilicate again, anhydrous sodium metasilicate add-on is 6% of fermented liquid weight, then adds calcium carbonate, and add-on is 20% of fermented liquid weight, with sulfuric acid or hydrochloric acid fermented liquid pH value be adjusted to 7 afterwards and place half an hour, last spraying dry.Spray drying condition is: inlet temperature 155 DEG C; Dry room temp 87 DEG C, temperature of outgoing air 67 DEG C, moisture eliminator internal pressure is-300MPa.Last monensin finished product content is 34%, particle rate 87%.
Embodiment 2
The preparation method of monensin premix is as follows:
A. seed culture
1) bacterial classification preparation is with embodiment 1.
2) seed flask is cultivated
Operation is with embodiment 1.
The substratum of shake-flask culture is made up of following ingredients by mass percentage concentration: soybean cake powder 1.4%, yeast powder 0.2%, glucose 0.35%, dextrin 1.5%, calcium carbonate 0.08%, and surplus is water.After medium sterilization, pH value is 7.0.
3) seed tank culture
By step 2) cultivate after bacterial classification add in seeding tank, inoculum size is 0.0035% seed liquor, is 32 ± 0.5 DEG C in temperature, and tank pressure is cultivate 23h under 0.04MPa condition, sampling detect.
Detected result: outward appearance is light yellow, thick, smell is normal, and without miscellaneous bacteria, pH value is 6.9, and biomass is 20%, and monensin is tired as 178U/mL, mycelia sturdy in netted, unfold, dye deeply, reach seed culture fluid quality control index.
Used medium is made up of following ingredients by mass percentage concentration: soybean cake powder 1.0%, yeast powder 0.15%, glucose 2.1%, calcium carbonate 0.2%, defoamer 0.1%, and surplus is water.Sterilizing wild Oryza species pH value is 6.7.
B. fermentation culture
By step 3) the bacterial classification culture transferring cultivated is in fermentor tank, and in tank, temperature maintains 34 ± 1 DEG C, and dissolved oxygen amount is more than 30%.By adding ammonia soln, vegetables oil and sterilized water in fermenting process, maintain between 6.5 ~ 6.6 to make fermented liquid pH value, vegetables oil is not less than 3% by mass percentage concentration, and dissolved oxygen amount is not less than 30%, and fermenting process terminates within first 24 ~ 48 hours, to stop adding vegetables oil; Ferment to 275 hours and terminate, sampling detects.
Detected result: pH value is 6.8, biomass is 50%, and monensin is tired as 51430u/mL, and Residual oil is 0.7%, and now mycelia starts disconnected joint, partial autolysis.
The substratum of described fermentation culture is counted by mass percentage concentration: glucose 3%, soybean cake powder 2.2%, vegetables oil 2.2%, Witconol 2301 1.0%, calcium carbonate 0.2%, sodium sulfate 0.2%, SODIUMNITRATE 0.2%, Manganous chloride tetrahydrate 0.01%, ferrous sulfate 0.01%, xitix 0.001%, Tai-Ace S 150 0.05%, dipotassium hydrogen phosphate 0.01%, defoamer 0.01%, surplus is water.Substratum NaOH adjust pH to 8, for subsequent use after constant volume sterilizing.
C. extract
First fermented liquid pH value is adjusted to 10.5, be then heated to 63 DEG C of insulations 3 hours, then add anhydrous sodium metasilicate, add-on is 6% of fermented liquid weight, adds calcium carbonate afterwards, and its add-on is 15% of fermented liquid weight.Again the pH value of fermented liquid be adjusted to 7.2 and place half an hour, last spraying dry.Spray drying condition is: inlet temperature 160 DEG C; Dry room temp 90 DEG C, temperature of outgoing air 72 DEG C, moisture eliminator internal pressure is-310 MPa.Last monensin finished product content is 30%, particle rate 80%.
It should be noted that, in leaching process calcium carbonate add-on with put tank and tire relevant, when putting tank, monensin is tired height, then calcium carbonate add-on increases.
What finally illustrate is, above preferred embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although by above preferred embodiment to invention has been detailed description, but those skilled in the art are to be understood that, various change can be made to it in the form and details, and not depart from claims of the present invention limited range.
Claims (7)
1. the preparation method of monensin premix, is characterized in that, comprises the following steps:
A. seed culture
The Chinese cassia tree prepared through bacterial classification ground streptomycete is carried out seed flask cultivation and seed tank culture successively; The culture condition that described seed flask is cultivated is the rotating speed with 220 ~ 260rpm, under 32 ± 0.5 DEG C of conditions, cultivate 24 ~ 27h; The inoculum size of described seed tank culture is 0.0035% seed liquor; The culture condition of described seed tank culture is for being 32 ± 0.5 DEG C in temperature, and tank pressure is cultivate 20 ~ 24h under 0.02 ~ 0.05MPa condition;
Substratum needed for described seed flask cultivation consists of the following composition by mass percentage concentration: soybean cake powder 1 ~ 1.5%, yeast powder 0.1 ~ 0.3%, glucose 0.2 ~ 0.5%, dextrin 1.0 ~ 2.0%, calcium carbonate 0.05 ~ 0.1%, and surplus is water; After medium sterilization, pH value is 6.9 ~ 7.1;
The substratum of described seed tank culture consists of the following composition by mass percentage concentration: soybean cake powder 1.0 ~ 1.5%, yeast powder 0.1 ~ 0.25%, glucose 1.5 ~ 2.5%, calcium carbonate 0.1 ~ 0.25%, defoamer 0.05 ~ 0.1%, and surplus is water; After medium sterilization, pH value is 6.6 ~ 7.1;
B. fermentation culture
The nutrient solution containing bacterial classification after being cultivated by step a proceeds in fermentor tank and carries out fermentation culture, and fermentation culture conditions is: temperature 34 ± 1 DEG C in tank, dissolved oxygen amount more than 30%, tank pressure 0.03 ~ 0.05MPa; By adding ammonia soln, vegetables oil and sterilized water in fermenting process, maintain between 6.5 ~ 6.6 to make fermented liquid pH value, vegetables oil is not less than 3% by mass percentage concentration, and dissolved oxygen amount is not less than 30%, and fermenting process terminates within first 24 ~ 48 hours, to stop adding vegetables oil; When fermented liquid pH value reaches 6.6 ~ 7.0, resid amount is less than 0.7%, biomass more than 40%, when monensin is tired and is not less than 40000U/mL, fermentation ends;
Fermention medium consists of the following composition by mass percentage concentration: glucose 2.0 ~ 3.8%, soybean cake powder 1.8 ~ 3.7%, vegetables oil 1.9 ~ 2.5%, Witconol 2301 0.05 ~ 1.0%, calcium carbonate 0.15 ~ 0.35%, sodium sulfate 0.15 ~ 0.22%, SODIUMNITRATE 0.15 ~ 0.22%, Manganous chloride tetrahydrate 0.01 ~ 0.03%, ferrous sulfate 0.005 ~ 0.01%, xitix 0.001 ~ 0.002%, Tai-Ace S 150 0.05 ~ 0.07%, dipotassium hydrogen phosphate 0.005 ~ 0.01%, defoamer 0.01 ~ 0.02%, surplus is water;
C. extract
After fermentation ends, first fermented liquid pH value is adjusted to 10 ~ 11, and then is heated to 63 DEG C and is incubated 3 hours, after adding anhydrous sodium metasilicate and calcium carbonate respectively, fermented liquid pH value is adjusted to 7 ~ 8 and places 30 minutes, last spraying dry; Described anhydrous sodium metasilicate add-on is 6% of fermented liquid weight, and described calcium carbonate add-on is 0 ~ 35% of fermented liquid weight; Described spray-dired condition is: inlet temperature 140 ~ 160 DEG C; Dry room temp 65 ~ 95 DEG C, temperature of outgoing air 60 ~ 70 DEG C, moisture eliminator internal pressure-280 ~-380MPa.
2. the preparation method of monensin premix according to claim 1, it is characterized in that, substratum needed for the cultivation of seed flask described in described step a consists of the following composition by mass percentage concentration: soybean cake powder 1.5%, yeast powder 0.2%, glucose 0.4%, dextrin 1.0%, calcium carbonate 0.06%, surplus is water.
3. the preparation method of monensin premix according to claim 1, it is characterized in that, described in described step a, the substratum of seed tank culture consists of the following composition by mass percentage concentration: soybean cake powder 1.5%, yeast powder 0.2%, glucose 2%, calcium carbonate 0.25%, defoamer 0.1%, surplus is water.
4. the preparation method of monensin premix according to claim 1, it is characterized in that, described in described step a, fermention medium consists of the following composition by mass percentage concentration: glucose 3%, soybean cake powder 3%, vegetables oil 2%, Witconol 2301 1.0%, calcium carbonate 0.2%, sodium sulfate 0.2%, SODIUMNITRATE 0.2%, Manganous chloride tetrahydrate 0.02%, ferrous sulfate 0.01%, xitix 0.001%, Tai-Ace S 150 0.06%, dipotassium hydrogen phosphate 0.01%, defoamer 0.01%, surplus is water.
5. the preparation method of monensin premix according to claim 1, it is characterized in that, the bacterial classification prepared through bacterial classification is carried out seed flask cultivation and seed tank culture by described step a successively; The culture condition that described seed flask is cultivated is the rotating speed with 220 ~ 260rpm, under 32 ± 0.5 DEG C of conditions, cultivate 25h; The inoculum size of described seed tank culture is 0.0035% seed liquor; The culture condition of described seed tank culture is for being 32 ± 0.5 DEG C in temperature, and tank pressure is cultivate 24h under 0.04MPa condition.
6. the preparation method of monensin premix according to claim 1, it is characterized in that, described step b be step a is cultivated after proceed in fermentor tank containing the nutrient solution of bacterial classification and carry out fermentation culture, fermentation culture conditions is: in tank, temperature is 34 ± 1 DEG C, dissolved oxygen amount is more than 30%, tank pressure is at 0.03MPa, by adding ammonia soln in fermenting process, vegetables oil and sterilized water, maintain between 6.5 ~ 6.6 to make fermented liquid pH value, vegetables oil is not less than 3% by mass percentage concentration, dissolved oxygen amount is not less than 30%, fermenting process terminates within first 24 ~ 48 hours, to stop adding vegetables oil, when fermented liquid pH is 6.9, resid amount is 0.6%, biomass 45%, when monensin is tired as 45690u/mL, fermentation ends.
7. the preparation method of monensin premix according to any one of claim 1 ~ 6, it is characterized in that, described step c is for be first adjusted to 10.8 by fermented liquid pH value, then be heated to 63 DEG C and be incubated 3 hours, after adding anhydrous sodium metasilicate and calcium carbonate, fermented liquid pH value be adjusted to 7 and place 30 minutes, last spraying dry; Described anhydrous sodium metasilicate add-on is 6% of fermented liquid weight, and described calcium carbonate add-on is 20% of fermented liquid weight; Described spray-dired condition is: inlet temperature 155 DEG C; Dry room temp 87 DEG C, temperature of outgoing air 67 DEG C, moisture eliminator internal pressure-300MPa.
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| CN106344520A (en) * | 2016-08-24 | 2017-01-25 | 浙江升华拜克生物股份有限公司 | Preparation method of monensin premix |
| CN110592159A (en) * | 2019-09-23 | 2019-12-20 | 浙江拜克生物科技有限公司 | Preparation process and device of monensin premix |
| CN112410386A (en) * | 2020-10-30 | 2021-02-26 | 内蒙古中牧生物药业有限公司 | Process for improving content of monensin A component |
| CN112626145A (en) * | 2021-01-15 | 2021-04-09 | 驻马店华中正大有限公司 | Production method of monensin for improving utilization rate of grease |
| CN112680489A (en) * | 2021-01-15 | 2021-04-20 | 驻马店华中正大有限公司 | Method for improving monensin bioavailability |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106344520A (en) * | 2016-08-24 | 2017-01-25 | 浙江升华拜克生物股份有限公司 | Preparation method of monensin premix |
| CN106344520B (en) * | 2016-08-24 | 2019-03-12 | 浙江拜克生物科技有限公司 | A kind of preparation method of monensin premix |
| CN110592159A (en) * | 2019-09-23 | 2019-12-20 | 浙江拜克生物科技有限公司 | Preparation process and device of monensin premix |
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| CN112410386A (en) * | 2020-10-30 | 2021-02-26 | 内蒙古中牧生物药业有限公司 | Process for improving content of monensin A component |
| CN112410386B (en) * | 2020-10-30 | 2021-08-31 | 内蒙古中牧生物药业有限公司 | Process for improving content of monensin A component |
| CN112626145A (en) * | 2021-01-15 | 2021-04-09 | 驻马店华中正大有限公司 | Production method of monensin for improving utilization rate of grease |
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| CN112626145B (en) * | 2021-01-15 | 2024-06-04 | 驻马店华中正大有限公司 | Monensin production method for improving grease utilization rate |
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