CN110592159B - Preparation process and device of monensin premix - Google Patents

Abstract

A preparation process of a monensin premix comprises the following steps: the method comprises the following steps: inoculating the sequentially cultured and activated streptomyces cinnamoneum strains, and then performing fermentation culture to obtain monensin fermentation liquor; step two: adding sodium hydroxide into the monensin fermentation liquor obtained in the step one to adjust the pH, stirring, and then carrying out solid-liquid separation to obtain a monensin wet solid; step three: drying the monensin wet solid obtained in the step two, and adding a carrier for mixing to obtain a mixture; step four: tabletting the mixture obtained in the step three to obtain a flaky material; step five: and d, finishing and sieving the flaky material obtained in the step four to obtain the finished product monensin premix. The preparation process adopted by the application can be automatically controlled in the production process, and has the advantages of no dust, low labor intensity and environmental protection in the production environment.

Description

Preparation process and device of monensin premix

Technical Field

The invention belongs to the technical field of feed additives, and particularly relates to a preparation process and a device of a monensin premix.

Background

Monensin, also called rumensin, is a substance secreted by microorganism streptomyces cinnamomea, is a monovalent polyether ion carrier type anticoccidial drug, has broad-spectrum anticoccidial effect, the sodium salt of the monensin can affect the ion balance of the polypide, causes the polypide to break and die, also has the functions of controlling the proportion of volatile fatty acid in the rumen, reducing the degradation of protein in the rumen, reducing the dry matter consumption of feed, improving the utilization rate of nutrient substances, improving the energy utilization rate of animals and the like, is widely added into the feed of ruminants, is mainly used for preventing and treating chicken, lamb, calf and rabbit coccidiosis and promoting the growth of premix of ruminants, the prior production technologies of monensin agents are mainly two, one technology is a solvent method, monensin fermentation liquor is obtained by fermentation, mycelium is obtained by filtering the fermentation liquor, and the mycelium is extracted by a solvent to obtain a pure product, then mixing the pure product with a carrier to obtain a monensin premix; another technique is a filtration-granulation method, for example, CN106344520A discloses a preparation method of a monensin premix, which comprises the steps of obtaining a monensin fermentation broth by fermentation, adjusting the pH of the fermentation broth with sodium hydroxide, adding a carrier to the fermentation broth, and then obtaining a mixed solution, performing spray granulation, and drying to obtain the monensin premix.

Disclosure of Invention

Aiming at the problems, the invention aims to provide a preparation process of a monensin premix, which can be automatically controlled in the production process, has no dust in the production environment, low labor intensity and environmental protection.

In order to achieve the purpose, the technical scheme of the invention is as follows:

a preparation process of a monensin premix sequentially comprises the following steps:

the method comprises the following steps: inoculating the activated streptomyces cinnamoneum strain which is subjected to seed bottle culture and seed tank culture in sequence, and then performing fermentation culture to obtain monensin fermentation liquor;

step two: heating the monensin fermentation liquor for a period of time, cooling to room temperature, adding sodium hydroxide to adjust the pH, fully stirring for 1-2 h, and performing solid-liquid separation to obtain a monensin wet solid;

step three: drying the monensin wet solid, adding a carrier after drying, and fully mixing to obtain a mixture of the monensin and the carrier;

step four: feeding the mixture into a tablet press for dry tabletting, feeding the flaky material into a granulator for granulation, and then feeding the flaky material into a grading sieve for sieving to obtain a finished product of the monensin premix;

step five: weighing and packaging the finished product monensin premix.

In the design of the scheme, through reasonable setting of process parameters, fermentation is carried out to obtain fermentation liquor with high monensin content, the pH of the fermentation liquor is adjusted by sodium hydroxide to enable monensin to become a monensin sodium salt, then solid-liquid separation is carried out to obtain wet solids, a carrier is added after the wet solids are dried to form a monensin premix mixture, and finally dry tabletting is carried out to prepare the monensin premix mixture into flaky materials, so that the problems of large dust and poor production environment in the traditional production process are solved, and then granulation, sieving and packaging are carried out.

Further, the water content of the mixture in the third step is 4-10% by mass; the bulk density of the mixture is 0.3-0.8 g/ml; the Carr index range of the mixture is 10-35%, and the optimal tabletting preparation conditions are adjusted according to the physicochemical properties and the powder properties of the mixture, so that the generation of dust is greatly reduced, the production environment is optimized, and the raw material loss is reduced.

Further, in the second step, the temperature of the monensin fermentation liquor is raised to 60-80 ℃, then the temperature is reduced to 20-30 ℃, and then sodium hydroxide is added to adjust the pH value to 7.0-10.0.

In the design of the scheme, the fermentation liquid is inactivated by heating firstly, so that substances such as protein in the fermentation liquid are denatured, and the fermentation can be finished accurately; if the fermentation is finished without heating, the metabolism of microorganisms in the fermentation solution before the solid-liquid separation process cannot be controlled; when the pH value is adjusted to 7.0-10.0 by using sodium hydroxide, the conversion effect of the monensin sodium salt is the best, the whole material is more uniform and has good fluidity, the subsequent solid-liquid separation is facilitated, and the uniformity and the quality stability of the monensin premix are also improved.

Further, the solid-liquid separation method in the step two is one or two of plate-frame separation and centrifuge separation.

Further, the drying method in the third step is one or more of boiling drying, fluidized bed drying, air flow drying and spray drying.

Further, the fermentation culture conditions in the first step are as follows: the culture temperature is 32-36 ℃, the pH value is 6.0-7.5, the pressure is 0.03-0.06 MPa, the stirring speed is 115-145 r/min, the ventilation volume is 1: 0.050-0.95, and the culture time is 350-430 h.

Preferably, the carrier in the third step is one or more of calcium carbonate, corncob powder, defatted rice bran, bran and zeolite powder, the carrier has low water content, strong adsorbability and stable chemical property, can be fully and uniformly mixed with the monensin sodium salt, is favorable for tabletting in a subsequent tabletting process, and the prepared premix has stable quality and is storage-resistant.

The utility model provides a fermenting installation suitable for preparation technology is mixed in advance to above-mentioned monensin can effectively improve the dissolved oxygen degree and be difficult for the gassing among the fermenting installation, can effectively improve fermentation efficiency, and this fermenting installation is including a jar body, set up at jar transmission shaft of internal portion, set up on jar body and right the transmission shaft carries out driven motor, strain pan feeding mouth and feed supplement mouth have been seted up on the jar body, discharge gate and air inlet have been seted up to jar body lower extreme, still be provided with in the jar body with the stirring unit of transmission shaft connection, just the stirring unit includes two at least stirring rakes and supplementary oar.

Further, the stirring rake includes axis of rotation and at least two sets of stirring leaves that constitute and be wavy periodic distribution in vertical direction, the stirring leaf is wavy periodic distribution in vertical direction, make the stirring rake when rotating, each stirring leaf forms the water wave with axis of rotation symmetric diffusion in each zymotic fluid layer, and the stirring rake is provided with two sets of symmetric distribution, the flow field of formation can be fully with energy diffusion to each position of zymotic fluid, thereby dissolved oxygen degree obtains improving, the striking of reducible stirring leaf of curved stirring leaf design and zymotic fluid simultaneously, reduce the production of bubble.

Furthermore, the auxiliary paddle comprises an auxiliary upright post and an auxiliary groove formed in the surface of the auxiliary upright post, the auxiliary groove is designed into an inwards-concave arc-shaped groove, when two opposite diffusion waves diffuse to the position near the auxiliary paddle, the arc-shaped groove can effectively absorb the energy of the diffusion waves, the energy of the center of the fermentation liquid near the auxiliary paddle is balanced with the energy of other positions of the fermentation liquid, the impact of water waves can be reduced through the arc-shaped groove, and the generation of bubbles is reduced.

Furthermore, a limiting unit is arranged and connected with the transmission shaft and the stirring unit respectively, and the limiting unit comprises a bottom plate connected with the transmission shaft and a fixing block arranged at the lower end of the bottom plate and used for fixing the bottom plate.

Further, be provided with the messenger on the bottom plate the passageway that the transmission shaft wore to establish and pass through, it is protruding to be provided with the block on the outside of transmission shaft, correspondingly, be provided with on the bottom plate passageway lateral wall with the block of the protruding looks adaptation of block, further preferably, the block is protruding and the block can be for semi-circular post through the cross section of mutual joint, can consolidate the protruding connection with the block of block, reduces rocking that the stirring in-process produced, guarantees stirring efficiency and reduces the production of bubble.

Preferably, the fixed block includes that both ends are in through the mode block of pegging graft stand on the bottom plate and with the slot of transmission shaft lower extreme looks adaptation, the fixed block is used for further strengthening the connection of transmission shaft and bottom plate.

Further, two stirring rakes set up the bottom plate both sides, supplementary oar sets up fixed block center.

Preferably, a paper clip type heating cavity is arranged on the tank body of the fermentation tank, and a heating element is arranged on the bottom surface of the paper clip type heating cavity.

Preferably, an insulating layer is arranged on the outer side of the tank body of the fermentation tank.

Compared with the prior art, the invention has the beneficial effects that:

the preparation method adopted by the invention does not need to use a large amount of organic solvent, does not generate dust, and has good production environment and environmental protection; the preparation method has the characteristics of high automation control degree and continuous production, greatly reduces the labor intensity, and has high production efficiency and high yield.

The wave-shaped stirring paddle is matched with the auxiliary paddle for absorbing energy, so that each liquid layer of the fermentation liquid can be effectively dissolved with oxygen, and bubbles are generated less, thereby being beneficial to fermentation of the fermentation liquid; the invention strengthens the connection between the stirring unit and the transmission shaft, reduces irregular shaking generated in the stirring process and ensures the stirring efficiency; according to the fermentation tank, the heating cavity arranged on the outer side of the tank body is matched with the heat-insulating layer to heat and insulate the fermentation tank, compared with the traditional heater arranged in the fermentation tank, the fermentation tank has the advantages that the mutual interference of fermentation liquor and the heater is reduced while the heating temperature control efficiency is equivalent, and the fermentation effect is improved.

Drawings

FIG. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic structural diagram of the limiting unit of the present invention.

Fig. 3 is a partial structural schematic diagram of the present invention.

Reference numerals: 1 tank body, 11 strain inlets, 12 feeding ports, 13 discharging ports, 14 air inlets, 2 motors, 3 transmission shafts, 31 clamping protrusions, 4 stirring units, 41 stirring paddles, 411 rotating shafts, 412 stirring blades, 42 auxiliary paddles, 421 auxiliary columns, 422 auxiliary grooves, 43 fixed bearings, 5 limiting units, 51 bottom plates, 511 channels, 512 clamping pieces, 52 fixed blocks, 521 columns, 522 slots, 6-turn heating cavities, 61 heating elements and 10 heat insulation layers.

Detailed Description

Example 1

Preparing fermentation liquor: inoculating the activated streptomyces cinnamoneum strain which is cultured in a seed bottle and a seed tank in sequence, and then performing fermentation culture to obtain monensin fermentation liquor; wherein, the culture conditions of the seed bottle culture are as follows: the culture temperature is 32 ℃, the rotating speed of a shaking table is 250r/min, and the culture time is 20 h; the culture conditions of the seeding tank culture are as follows: the culture temperature is 32 ℃, the pH value is 6.0, the tank pressure is 0.04MPa, the stirring speed is 200r/min, the ventilation volume is 1:0.65, and the culture time is 26 h; the culture conditions of the fermentation culture are as follows: the culture temperature is 32 ℃, the pH value is 6.0, the tank pressure is 0.03MPa, the stirring speed is 130r/min, the ventilation volume is 1:0.70, and the culture time is 390 h.

Preparation of monensin wet solid: and (2) heating the prepared monensin fermentation liquor to 70 ℃ and continuing until protein and other substances in the fermentation liquor are inactivated and denatured, generally requiring 2-4 hours, then cooling to 25 ℃, adding a sodium hydroxide solution to adjust the pH to about 9.0, fully and uniformly stirring, and performing solid-liquid separation after the pH value is stable, wherein the solid-liquid separation can be plate-and-frame filtration to obtain the monensin wet solid.

Preparing a mixture: drying the obtained monensin wet solid in a manner of one or more of fluidized drying, fluidized bed drying, airflow drying and spray drying which are commonly used in industrial processes, wherein the drying manner is not limited, the mass percent of water in the dried solid is less than 10%, and then adding the carrier corncob powder for uniformly mixing, so that the bulk density of the final mixture is within the range of 0.3-0.8 g/ml, and the Carr index is within the range of 10-35%.

Preparing a finished product monensin premix: and conveying the obtained mixture to a tablet press for dry tabletting, conveying the obtained flaky material into a granulating machine for granulating, conveying the flaky material into a grading sieve for sieving, and obtaining the finished product monensin premix meeting the requirements.

Example 2

The difference in the preparation of the fermentation broth in this example from example 1 is that:

the culture conditions of the seed bottle culture are as follows: the culture temperature is 33 ℃, the rotating speed of a shaking table is 260r/min, and the culture time is 18 h; the culture conditions of the seeding tank culture are as follows: the culture temperature is 33 ℃, the pH value is 7.5, the tank pressure is 0.06MPa, the stirring speed is 220r/min, the ventilation volume is 1:0.88, and the culture time is 20 h; the culture conditions of the fermentation culture are as follows: the culture temperature is 36 ℃, the pH value is 7.5, the tank pressure is 0.06MPa, the stirring speed is 145r/min, the ventilation volume is 1:0.95, and the culture time is 350 h.

Preparation of monensin wet solid: and (2) heating the prepared monensin fermentation liquor to 80 ℃ and continuing until protein and other substances in the fermentation liquor are inactivated and denatured, generally requiring 2-4 h, then cooling to 20 ℃, adding a sodium hydroxide solution to adjust the pH to about 7.0, fully and uniformly stirring, and performing solid-liquid separation after the pH value is stable, wherein the solid-liquid separation can be plate-and-frame filtration to obtain the monensin wet solid.

The carrier used in this example was calcium carbonate.

Example 3

The difference in the preparation of the fermentation broth in this example from example 1 is that:

the culture conditions of the seed bottle culture are as follows: the culture temperature is 35 ℃, the rotating speed of a shaking table is 280r/min, and the culture time is 22 h; the culture conditions of the seeding tank culture are as follows: the culture temperature is 35 ℃, the pH value is 6.0, the tank pressure is 0.06MPa, the stirring speed is 240r/min, the ventilation volume is 1:1, and the culture time is 30 h; the culture conditions of the fermentation culture are as follows: the culture temperature is 34 ℃, the pH value is 7.0, the tank pressure is 0.04MPa, the stirring speed is 115r/min, the ventilation volume is 1:0.05, and the culture time is 430 h.

Preparation of monensin wet solid: and (2) heating the prepared monensin fermentation liquor to 60 ℃ and continuing until protein and other substances in the fermentation liquor are inactivated and denatured, generally requiring 2-4 hours, then cooling to 30 ℃, adding a sodium hydroxide solution to adjust the pH to about 10.0, fully and uniformly stirring, and performing solid-liquid separation after the pH value is stable, wherein the solid-liquid separation can be plate-and-frame filtration to obtain the monensin wet solid.

The carrier used in this example was zeolite powder.

The results of the preparations of examples 1 to 3 were analyzed:

according to the preparation method based on CN106344520A, the monensin premix which meets the specification and is prepared in the embodiments 1-3 is stable in quality, durable in storage, less in dust generation in the preparation process, green and environment-friendly, has the characteristic of continuous production, and improves the production efficiency.

Example 4

The embodiment provides a fermentation device suitable for preparation of a monensin premix, as shown in fig. 1, the fermentation device comprises a tank body 1 and a transmission shaft 3 arranged in the middle of the inside of the tank body 1, a motor 2 is arranged at the top of the tank body 1, an output end of the motor is connected with the transmission shaft 3, a strain inlet 11 and a feed supplement port 12 are arranged at the upper end of the tank body 1, a discharge port 13 and an air inlet 14 for compressed air to enter are arranged at the lower end of the tank body 1, control valves are arranged at the strain inlet 11, the feed supplement port 12, the discharge port 13 and the air inlet 14, a square-shaped heating cavity 6 is arranged at the middle lower part of the tank body 1, a heating element 61 is arranged at the bottom inside of the square-shaped heating cavity 6, the heat conducting element is connected with an external storage battery to heat water or oil liquid in the square-shaped heating cavity through, thereby reducing energy consumption during fermentation.

In this embodiment, as shown in fig. 1 and 2, it is provided with the bottom plate 51 and is connected with the transmission shaft 3, the stirring paddles 41 are symmetrically arranged on both sides of the lower surface of the bottom plate 51, the center of the lower surface of the bottom plate 51 is provided with the auxiliary paddle 42, the stirring paddles 41 include the rotating shaft 411 and six groups of stirring blades 412 which are arranged in a wave-shaped cycle in the vertical direction, the auxiliary paddle includes the auxiliary column 421 and the auxiliary groove 422 which is arranged on the surface of the auxiliary column 421, and the fixed bearing 43 which is connected with the bottom plate 51 is sleeved on the connecting positions of the stirring paddles 41 and the auxiliary paddle 42.

In this embodiment, the connection mode not illustrated may be a common screw connection, welding, hinge connection, clamping connection, or the like.

The working principle of the embodiment is as follows: will treat the material and the bacterial of fermentation respectively through feed supplement mouth 12, bacterial entry 11 is carried to jar internal 1, thereby motor 2 drive transmission shaft 3 rotates stirring rake and the supplementary oar rotation on the bottom plate 51 of being connected with transmission shaft 3 and carries out the intensive mixing to the zymotic fluid, simultaneously through the battery through heating member 61 to the water or fluid of type heating intracavity of returning, make type heating chamber 6 keep warm to jar body 1, heat preservation 10 plays the synchronous heat preservation effect to jar body 1 simultaneously, in the fermentation process, carry the pressure and the air output of compressed gas control jar body 1 through air inlet 14, adjust optimum fermentation condition, after the fermentation is accomplished, carry the fermented material out of jar body 1 through discharge gate 13.

Example 5

The difference between this embodiment and embodiment 4 is that, as shown in fig. 2, a fixing block 52 for connecting the reinforcing bottom plate 51 and the transmission shaft is disposed at the center of the lower surface of the bottom plate 51, the center of the lower surface of the fixing block 52 is connected with the auxiliary paddle 42, a slot 522 adapted to the bottom surface of the transmission shaft 3 is disposed on the upper surface of the fixing block 52, and the upper surface of the fixing block 52 is further symmetrically provided with a vertical column 521 for being inserted into the lower surface of the bottom plate 51, so as to further enhance the fixing effect, a magnetic block may be disposed at the upper end of the vertical column 521, and correspondingly, a magnetic block for generating a magnetic force for mutual.

In this embodiment, as shown in fig. 3, the outer surface of the transmission shaft 3 is provided with a clamping protrusion 31, and correspondingly, the inner side surface of the channel 511 formed in the bottom plate 51 is provided with a clamping member 512 which is in contact with and clamped with the clamping protrusion, specifically, the clamping protrusion 511 and the clamping member 512 are of a column structure with a semicircular cross section, which can further strengthen the connection between the transmission shaft and the bottom plate 51 and reduce the shaking of the motor during operation.

The working principle of the embodiment is as follows: the material and the bacterial of waiting to ferment respectively through feed supplement mouth 12, bacterial entry 11 is carried to jar body 1 in, thereby motor 2 drive transmission shaft 3 rotates the stirring rake of drive connection on the bottom plate 51 of being connected with transmission shaft 3 and fixes and rotate the supplementary oar of fixing the fixed block lower extreme of being connected with bottom plate 51 lower surface and carry out the intensive mixing to the zymotic fluid, simultaneously through the battery through heating member 61 to the water or fluid of returning the type heating intracavity heat, make returning the type heating chamber 6 keep warm to jar body 1, heat preservation 10 plays the heat preservation effect to jar body 1 in step simultaneously, in the fermentation process, carry the pressure and the air output of compressed gas control jar body 1 through air inlet 14, adjust optimum fermentation condition, after the fermentation is accomplished, carry the fermented product out jar body 1 through discharge gate 13.

The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or ambit of the invention as defined in the appended claims.

Claims (2)

1. The utility model provides a fermenting installation suitable for monensin mixes agent preparation technology in advance, a serial communication port, including jar body (1), set up in jar transmission shaft (3) of body (1) inside, set up and be in jar body (1) is gone up and right transmission shaft (3) carry out driven motor (2), strain entry (11) and feed supplement mouth (12) have been seted up on jar body (1), discharge gate (13) and air inlet (14) have been seted up to the lower extreme of jar body (1), still be provided with in jar body (1) with stirring unit (4) that transmission shaft (3) are connected, stirring unit (4) include two at least stirring rakes (41) and an auxiliary paddle (42), stirring rake (41) include axis of rotation (411) and two sets at least constitution stirring leaf (412) that are wavy periodic distribution in vertical direction.

2. The fermentation device suitable for the preparation process of the monensin premix according to claim 1, wherein the auxiliary paddle (42) comprises an auxiliary column (421) and an auxiliary groove (422) formed on the surface of the auxiliary column.

Images