CN112680489A - Method for improving monensin bioavailability - Google Patents
Method for improving monensin bioavailability Download PDFInfo
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- CN112680489A CN112680489A CN202110057680.0A CN202110057680A CN112680489A CN 112680489 A CN112680489 A CN 112680489A CN 202110057680 A CN202110057680 A CN 202110057680A CN 112680489 A CN112680489 A CN 112680489A
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- 229930191564 Monensin Natural products 0.000 title claims abstract description 43
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- 229960005358 monensin Drugs 0.000 title claims abstract description 43
- GAOZTHIDHYLHMS-KEOBGNEYSA-N monensin A Chemical compound C([C@@](O1)(C)[C@H]2CC[C@@](O2)(CC)[C@H]2[C@H](C[C@@H](O2)[C@@H]2[C@H](C[C@@H](C)[C@](O)(CO)O2)C)C)C[C@@]21C[C@H](O)[C@@H](C)[C@@H]([C@@H](C)[C@@H](OC)[C@H](C)C(O)=O)O2 GAOZTHIDHYLHMS-KEOBGNEYSA-N 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 41
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- 102000016943 Muramidase Human genes 0.000 claims abstract description 10
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- 239000001963 growth medium Substances 0.000 claims description 22
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 238000011218 seed culture Methods 0.000 claims description 18
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 15
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
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- QYDYPVFESGNLHU-UHFFFAOYSA-N elaidic acid methyl ester Natural products CCCCCCCCC=CCCCCCCCC(=O)OC QYDYPVFESGNLHU-UHFFFAOYSA-N 0.000 claims description 3
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- QYDYPVFESGNLHU-KHPPLWFESA-N methyl oleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC QYDYPVFESGNLHU-KHPPLWFESA-N 0.000 claims description 3
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- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims description 3
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Abstract
The invention belongs to the technical field of monensin fermentation, and particularly discloses a method for improving the bioavailability of monensin. The method increases the lysozyme treatment cost, but reduces the sewage discharge, and the extraction stage really realizes zero discharge and is environment-friendly.
Description
Technical Field
The invention belongs to the technical field of fermentation of monensin, and particularly relates to a method for improving the bioavailability of monensin.
Background
Monensin interferes with the normal exchange of sodium and potassium ions in coccidian cells, so that a large amount of sodium ions enter the cells, the intracellular concentration of the sodium ions is high, the osmotic pressure is increased, and more water enters the coccidian. Monensin can affect energy metabolism in rumen of ruminant, improve rumen fermentation, increase output ratio of propionic acid and acetic acid, reduce volatile fatty acid, reduce methane generation, and improve protein utilization efficiency. In the case of the current ban on the addition of growth-promoting antibiotics to the feed, monensin can continue to be used as an anti-coccidial therapeutic effect. The product characteristics are as follows: 1. the stability is high, the coccidian control effect is good, the medicine is the first choice when the medicine is used alternately, and the effect is stable and the time is tested for all poultry; 2. the feed efficiency is improved, monensin can help broilers to be slaughtered in advance, the feed conversion ratio is improved, and the economic benefit is obvious; 3. Stimulating coccidiosis immunity; however, the existing production processes are all the phenomena that after fermentation is finished, thalli are directly collected and granulated to obtain the monensin premix product, and gastrointestinal dissolution experiments and bioavailability experiments show that the monensin premix product has low release rate of the monensin in the thalli, so that the bioavailability of the product is low.
Disclosure of Invention
The invention aims to provide a method for improving the bioavailability of monensin, and the preparation method of the monensin premix changes the traditional production process, does not need plate-and-frame filter pressing, directly sprays and dries the fermentation liquor after partial enzymolysis to produce the monensin product, reduces the generation of sewage and the use of equipment, utilizes the residual nutrient components after the fermentation is finished, saves the cost and improves the utilization rate of the equipment.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for improving the bioavailability of monensin adopts a culture medium for producing monensin by fermenting Streptomyces cinnamomea, the fermentation process is three-stage, and the method comprises first-stage seed culture, second-stage seed culture and fermentation culture and comprises the following steps:
1) the first-stage seed culture adopts a culture medium for culture, and the first-stage seed culture medium comprises the following components: 5g/L of glucose, 20g/L of dextrin, 15g/L of soybean meal, 3g/L of yeast powder and 5g of corn protein powder, wherein the culture process period is 20-30h, the culture temperature is 30-34 ℃, the rotating speed is 200rpm, the seed transfer index is pH 6.6-7.1, and the bacterial concentration is 15-20%;
2) the second-stage seed culture adopts a culture medium for culture, and the composition of the second-stage seed culture medium is as follows: 5g/L of glucose, 2g/L of calcium carbonate, 15g/L of soybean meal and 3g/L of yeast powder, 0.04% of first-stage inoculation second-stage inoculation amount of the culture process, 10-20h of culture period, 30-34 ℃ of temperature, 200rpm of rotating speed, 0.5-1.0vvm of ventilation and seed transplanting index: pH 6.6-7.1, bacterial concentration 15-20%;
3) the fermentation culture adopts a fermentation tank culture medium, the fermentation tank culture medium comprises 20g/L of glucose, 20g/L of soybean meal, 10g/L of methyl oleate, 20g/L of soybean oil, 2g/L of sodium nitrate, 2g/L of sodium sulfate, 0.1g/L of dipotassium hydrogen phosphate, 0.3g/L of manganese chloride, 0.02g/L of ascorbic acid, 3g/L of light calcium carbonate and 0.2g/L of sodium silicate, and the culture process comprises the following steps: the inoculation amount is 8.0 percent, and the period is as follows: 300h, the temperature is 30-34 ℃, the rotating speed is 100rpm, the ventilation volume is 0.5-1.0vvm, the pH of the culture solution is kept at 6.5 and the oil control level is 1.0-2.0% by adding a supplemented medium in the fermentation culture process;
4) extracting and preparing a finished product: adding lysozyme with the weight of 0.05 percent into the fermentation liquor after the fermentation in the step 3), keeping the temperature for reaction at 40 ℃ for 30-60min, heating to 60-80 ℃, stirring at the speed of 100 plus 200rpm to ensure that the enzymolysis rate is 30-40 percent, finishing the enzymolysis, performing partial wall breaking treatment on the monensin mycelium by the lysozyme, wherein the partial wall breaking treatment is because the enzyme amount required by the mycelium is larger when all the walls are broken and the treatment time is long, causing high cost, hardly ensuring all the walls are broken, heating to 70 ℃, adding the mixture into a pretreatment tank, adding sodium hydroxide from a tank opening, uniformly stirring to ensure that the pH value of the solution is 9.0-10, adding calcium carbonate, stirring for 35 min, adding all the feed liquid into a premixing tank, starting a pressure spray drying tower, adjusting the pressure of an air compressor to O.35MPa, the feeding flow to be 300L/h, and the air inlet temperature to be 120 plus 180 ℃, the air outlet temperature is 60-90 ℃, the discharge port temperature is 50-60 ℃, and the product is collected.
Further, the feed medium in the step 3) is at least one of palm oil, 18-23% of ammonia water, 10% of liquid alkali, 0.5% of ammonium sulfate and 0.05% of phosphoric acid mixed solution.
Further, the adding amount of the calcium carbonate in the step 4) is +/-5% of the calculated adding amount of the calcium carbonate.
A monensin premix product prepared by the method for improving the bioavailability of monensin.
The invention has the advantages that: the preparation method of the monensin premix changes the traditional production process, does not need plate-frame filter pressing, directly sprays and dries the zymotic fluid after partial enzymolysis to produce the monensin product, reduces the production of sewage, reduces the use of equipment, utilizes the residual nutrient components after the fermentation is finished, saves the cost, improves the utilization rate of the equipment, increases a lysozyme to carry out partial wall breaking treatment on the monensin mycelium in the extraction stage, releases partial product to be dissociated outside cells, finally directly sprays and dries the zymotic fluid through a pressure spray drying tower for granulation, carries out bioavailability test on the product prepared by the improved process and the product prepared by the original process, collects blood after the medicine is put into the product, detects the blood medicine concentration at different time points, and the experimental result shows that the peak reaching time of animals is shorter after the improved process medicine is orally taken, the absorption speed is high, the method increases the lysozyme treatment cost, reduces sewage discharge, really achieves zero discharge in the extraction stage, and is environment-friendly.
Drawings
FIG. 1 is a graph comparing the blood concentration-time curve of the experimental group and the control group in the example of the present invention.
Detailed Description
Examples
A method for improving the bioavailability of monensin adopts a culture medium for producing monensin by fermenting Streptomyces cinnamomea, the fermentation process is three-stage, and the method comprises first-stage seed culture, second-stage seed culture and fermentation culture and comprises the following steps:
1) the first-stage seed culture adopts a culture medium for culture, and the first-stage seed culture medium comprises the following components: 5g/L of glucose, 20g/L of dextrin, 15g/L of soybean meal, 3g/L of yeast powder and 5g of corn protein powder, wherein the culture process period is 20-30h, the culture temperature is 30-34 ℃, the rotating speed is 200rpm, the seed transfer index is pH 6.6-7.1, and the bacterial concentration is 15-20%;
2) the second-stage seed culture adopts a culture medium for culture, and the composition of the second-stage seed culture medium is as follows: 5g/L of glucose, 2g/L of calcium carbonate, 15g/L of soybean meal and 3g/L of yeast powder, 0.04% of first-stage inoculation second-stage inoculation amount of the culture process, 10-20h of culture period, 30-34 ℃ of temperature, 200rpm of rotating speed, 0.5-1.0vvm of ventilation and seed transplanting index: pH 6.6-7.1, bacterial concentration 15-20%;
3) the fermentation culture adopts a fermentation tank culture medium, the fermentation tank culture medium comprises 20g/L of glucose, 20g/L of soybean meal, 10g/L of methyl oleate, 20g/L of soybean oil, 2g/L of sodium nitrate, 2g/L of sodium sulfate, 0.1g/L of dipotassium hydrogen phosphate, 0.3g/L of manganese chloride, 0.02g/L of ascorbic acid, 3g/L of light calcium carbonate and 0.2g/L of sodium silicate, and the culture process comprises the following steps: the inoculation amount is 8.0 percent, and the period is as follows: 300h, the temperature is 30-34 ℃, the rotation speed is 100rpm, the ventilation volume is 0.5-1.0vvm, the fermentation culture process maintains the pH of the culture solution to be 6.5 by adding a supplemented medium, the oil control level is 1.0-2.0%, the supplemented medium is at least one of palm oil, 18-23% ammonia water, 10% caustic soda liquid, 0.5% ammonium sulfate and 0.05% phosphoric acid mixed solution;
4) extracting and preparing a finished product: adding 0.05 percent of lysozyme into the fermentation liquor after the fermentation in the step 3), keeping the temperature for reaction at 40 ℃ for 30-60min, heating to 60-80 ℃, stirring at the speed of 100 plus 200rpm to ensure that the enzymolysis rate is finished at 30-40 percent, heating to 70 ℃, pumping into a pretreatment tank, adding sodium hydroxide from the tank mouth, uniformly stirring to ensure that the pH value of the solution is 9.0-10, adding calcium carbonate, stirring for 35 min, wherein the addition of the calcium carbonate is +/-5 percent of the calculated value of the addition of the calcium carbonate, and because the lysozyme carries out partial wall breaking treatment on the monensin mycelium, the partial wall breaking treatment is because the enzyme quantity required by the mycelium is larger when all the walls are broken, the cost is high because the treatment time is long, in addition, the whole wall breaking is difficult to ensure, pumping the feed liquid into a premixing tank, starting a pressure spray drying tower, adjusting the pressure of an air compressor to be O.35MPa, and the feed flow rate is 300L/, the air inlet temperature is 120-180 ℃, the air outlet temperature is 60-90 ℃, the discharge port temperature is 50-60 ℃, and the product is collected.
A monensin premix product prepared by the method for improving the bioavailability of monensin.
Comparative experiment
Firstly, a proper hydrolysis process and lysozyme are found, the importance of the influencing factors is evaluated, the factors are tested and verified, and the optimal reaction conditions are determined. Through single factor analysis, the hydrolysis process is respectively determined to be that the added enzyme amount is 0.05 percent, the hydrolysis temperature is 40 ℃, the reaction time is 1 hour, and the pH and the rotating speed are natural (the enzyme activity unit is 1 ten thousand units/ml). The enzymolysis rate is the ratio of free drug potency to total potency, and the specific results are shown in table 1.
TABLE 1
The process is characterized in that after the slant spores are scraped by an inoculation needle, the inoculation needle is inoculated to first-stage shake flask seeds (40 ml of seed liquid), the culture temperature is 30-32 ℃, the rotation speed is 200rpm, the culture time is about 20 hours, the seed transfer index is about pH7.0, the fungus concentration is not lower than 15%, the microscopic hypha Meilan is deep and thick, and the hypha is long and spread; the liquid volume of the second-stage canning is 100L, the inoculation amount is 0.04%, the culture temperature is 32 ℃, the rotating speed is 200rpm, the ventilation volume is 0.5-1.0vvm, when the pH is 6.6-7.0, the bacteria concentration is not lower than 16%, and the culture period is 16-24 h; the liquid loading of the fermentation tank is 500L, the fermentation inoculation amount is 8% (40L of secondary seeds is needed), the culture temperature is 30-35 ℃, the rotating speed is 130rpm, and the ventilation volume is 0.4-1.0vvm and is not less than 30% according to the dissolved oxygen. The fermentation process detects the bacterial concentration, pH, amino nitrogen, ammonia nitrogen, total sugar, residual oil and titer. And (5) detecting the titer and residual oil after the fermentation is finished.
The improved monensin premix of example 1 was prepared as follows
(1) 13 tons of monensin fermentation liquor with the titer of 60000U/ml are put in a fermentation tank, the process of hydrolyzing palm oil by lysozyme is added, the addition amount is 0.05 percent, the heat preservation reaction is carried out for 30-60min at 40 ℃, the stirring is carried out at 200rpm, and the enzymolysis rate is ensured to be 30-40 percent. Heating to 70 ℃ after enzymolysis, putting into a pretreatment tank, adding sodium hydroxide from the tank opening, uniformly stirring until the pH value of the solution is 9.0-;
(2) pumping the feed liquid in the step (2) into a premixing tank, starting a pressure spray drying tower, adjusting the pressure of an air compressor to be O.35MPa, the feeding flow to be 300L/h, the air inlet temperature to be 120-;
(3) considering that the allowable monensin addition amount in the feed in China is only 20mg/kg, and the detection lower limit of the monensin ELISA kit is 0.05mg/kg, the test adopts a one-time administration mode, and the medicine adding amount is 10mg/kg for each test pig.
(4) Blood sample collection and processing
During the test period, all test pigs are fasted, and the free drinking water blood sampling time points are respectively 0 hour, 0.5 hour, 1 hour, 1.5 hours, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 8 hours, 10 hours, 12 hours, 14 hours, 17 hours, 20 hours and 24 hours after the medicine administration, and the blood sampling method is to sample blood from the front cavity vein of the pig for 16 times, wherein each time is 4-5 ml;
(5) results and analysis
And (3) carrying out blood concentration detection on the sampled product, drawing a blood concentration-time curve according to the content of the monensin in pig blood serum of a control group and an experimental group, and obtaining an analysis result shown in figure 1.
The experimental results show that the absorption speed of the drug in the experimental group (the drug after the process improvement) is higher than that of the control group, the maximum value is reached in a short time, the high blood concentration is maintained for a long time, the absorption process of the product prepared by adding a part of wall breaking process in the animal body has a certain promotion effect, and the bioavailability of the monensin is improved.
The preparation method of the monensin premix changes the traditional production process, does not need plate-and-frame filter pressing, directly sprays and dries the fermentation liquor after partial enzymolysis to produce the monensin product, reduces the generation of sewage and the use of equipment, utilizes the residual nutrient components after the fermentation is finished, saves the cost and improves the utilization rate of the equipment.
Claims (4)
1. A method for improving the bioavailability of monensin adopts a culture medium for producing monensin by fermenting Streptomyces cinnamomea, the fermentation process is three-stage, and the method comprises first-stage seed culture, second-stage seed culture and fermentation culture, and is characterized by comprising the following steps of:
1) the first-stage seed culture adopts a culture medium for culture, and the first-stage seed culture medium comprises the following components: 5g/L of glucose, 20g/L of dextrin, 15g/L of soybean meal, 3g/L of yeast powder and 5g of corn protein powder, wherein the culture process period is 20-30h, the culture temperature is 30-34 ℃, the rotating speed is 200rpm, the seed transfer index is pH 6.6-7.1, and the bacterial concentration is 15-20%;
2) the second-stage seed culture adopts a culture medium for culture, and the composition of the second-stage seed culture medium is as follows: 5g/L of glucose, 2g/L of calcium carbonate, 15g/L of soybean meal and 3g/L of yeast powder, 0.04% of first-stage inoculation second-stage inoculation amount of the culture process, 10-20h of culture period, 30-34 ℃ of temperature, 200rpm of rotating speed, 0.5-1.0vvm of ventilation and seed transplanting index: pH 6.6-7.1, bacterial concentration 15-20%;
3) the fermentation culture adopts a fermentation tank culture medium, the fermentation tank culture medium comprises 20g/L of glucose, 20g/L of soybean meal, 10g/L of methyl oleate, 20g/L of soybean oil, 2g/L of sodium nitrate, 2g/L of sodium sulfate, 0.1g/L of dipotassium hydrogen phosphate, 0.3g/L of manganese chloride, 0.02g/L of ascorbic acid, 3g/L of light calcium carbonate and 0.2g/L of sodium silicate, and the culture process comprises the following steps: the inoculation amount is 8.0 percent, and the period is as follows: 300h, the temperature is 30-34 ℃, the rotating speed is 100rpm, the ventilation volume is 0.5-1.0vvm, the pH of the culture solution is kept at 6.5 and the oil control level is 1.0-2.0% by adding a supplemented medium in the fermentation culture process;
4) extracting and preparing a finished product: adding 0.05 percent of lysozyme into the fermentation liquor after the fermentation in the step 3), keeping the temperature for reaction at 40 ℃ for 30-60min, heating to 60-80 ℃, stirring at the speed of 100 plus 200rpm to ensure that the enzymolysis rate is 30-40 percent after the enzymolysis is finished, heating to 70 ℃, pumping into a pretreatment tank, adding sodium hydroxide from the tank opening, uniformly stirring to ensure that the pH value of the solution is 9.0-10, adding calcium carbonate, stirring for 35 min, pumping all the feed liquid into a premixing tank, starting a pressure spray-drying tower, adjusting the pressure of an air compressor to O.35MPa, feeding the flow rate to 300L/h, the air inlet temperature to 120 plus 180 ℃, the air outlet temperature to 60-90 ℃, the discharge outlet temperature to 50-60 ℃, and collecting the product.
2. The method of claim 1, wherein the administration of monensin comprises: the feed supplement culture medium in the step 3) is at least one of palm oil, 18-23% of ammonia water, 10% of liquid caustic soda, 0.5% of ammonium sulfate and 0.05% of phosphoric acid mixed liquor.
3. The method of claim 2, wherein the administration of monensin comprises: the addition amount of the calcium carbonate in the step 4) is +/-5% of the calculated addition amount of the calcium carbonate.
4. A monensin premix product prepared by the method for improving the bioavailability of monensin according to any one of claims 1 to 3.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116410958A (en) * | 2023-02-09 | 2023-07-11 | 驻马店华中正大有限公司 | Preparation method of lysozyme inclusion particles and lysozyme inclusion particles |
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| CN103937848A (en) * | 2014-04-14 | 2014-07-23 | 宁夏泰瑞制药股份有限公司 | Novel culture medium for producing monensin by fermenting streptomyces cinnamonensis and culture method |
| CN104313079A (en) * | 2014-11-03 | 2015-01-28 | 金河生物科技股份有限公司 | Preparation method of monensin premix |
| CN106344520A (en) * | 2016-08-24 | 2017-01-25 | 浙江升华拜克生物股份有限公司 | Preparation method of monensin premix |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103937848A (en) * | 2014-04-14 | 2014-07-23 | 宁夏泰瑞制药股份有限公司 | Novel culture medium for producing monensin by fermenting streptomyces cinnamonensis and culture method |
| CN104313079A (en) * | 2014-11-03 | 2015-01-28 | 金河生物科技股份有限公司 | Preparation method of monensin premix |
| CN106344520A (en) * | 2016-08-24 | 2017-01-25 | 浙江升华拜克生物股份有限公司 | Preparation method of monensin premix |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116410958A (en) * | 2023-02-09 | 2023-07-11 | 驻马店华中正大有限公司 | Preparation method of lysozyme inclusion particles and lysozyme inclusion particles |
| CN116410958B (en) * | 2023-02-09 | 2024-06-04 | 驻马店华中正大有限公司 | Preparation method of lysozyme inclusion particles and lysozyme inclusion particles |
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