CN1273784A - Process for breaking wall of pollen - Google Patents
Process for breaking wall of pollen Download PDFInfo
- Publication number
- CN1273784A CN1273784A CN00105084A CN00105084A CN1273784A CN 1273784 A CN1273784 A CN 1273784A CN 00105084 A CN00105084 A CN 00105084A CN 00105084 A CN00105084 A CN 00105084A CN 1273784 A CN1273784 A CN 1273784A
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- pollen
- radiation
- wall
- processing
- breaking
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Links
- 238000000034 method Methods 0.000 title claims abstract description 19
- 230000008569 process Effects 0.000 title claims abstract description 7
- 230000005855 radiation Effects 0.000 claims abstract description 26
- 235000013305 food Nutrition 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims description 3
- 238000009930 food irradiation Methods 0.000 claims description 3
- 239000005022 packaging material Substances 0.000 claims description 2
- 238000012856 packing Methods 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000012545 processing Methods 0.000 description 17
- 230000001413 cellular effect Effects 0.000 description 12
- 241000628997 Flos Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- 238000002372 labelling Methods 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 239000000084 colloidal system Substances 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- -1 flavone compound Chemical class 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000002110 toxicologic effect Effects 0.000 description 2
- 231100000027 toxicology Toxicity 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000009172 bursting Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000007198 pollen germination Effects 0.000 description 1
- 239000012804 pollen sample Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 201000007094 prostatitis Diseases 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Jellies, Jams, And Syrups (AREA)
Abstract
A process for scrifying pollen features that the fresh or dried pollen is loaded in the container made of radiation-resistant food packing material and radiated under 1-11 KGy for 24 hrs. Its advantages include high wall-broken rate, sterilizing effect, no loss of nutrients, high efficiency and no environmental pollution.
Description
The present invention relates to a kind of wall-breaking method of pollen.
Pollen is the male gametophyte of plant sexual propagation.The nutritional labeling of pollen is very abundant, contains protein, aminoacid, saccharide, lipid, vitamin, nucleic acid, mineral, enzyme and flavone compound etc.Pollen has effects such as resistance prostatitis, defying age, anti-hypoxia, blood fat reducing, is widely used in aspects such as food, beverage, cosmetics, medicine, feedstuff.But pollen has a hard pollen wall, and the main component of pollen wall is sporopollenin, cellulose, carotenoid etc., and acidproof anticorrosive, the pollen main nutrient composition is present in the inclusions of pollen wall inside.Pollen has only the nutritional labeling of its inclusions behind breaking cellular wall to be digested and assimilated by body.
At present, the wall-breaking method of pollen mainly contains following several:
1) breaking cellular wall by temperature difference method: utilize the principle of expanding with heat and contract with cold, pollen drops into rapidly after freezing in the hot water " bursting ", and this method is destroyed bigger to the nutritional labeling of pollen, and complicated operation, and the pollen behind the breaking cellular wall also needs dry if will make powder.
2) colloid mill breaking cellular wall method: be to place-15～-25 ℃ to descend freezing 15 hours mixed pollen, pollen after freezing put in the water-bath melt, again by the colloid mill breaking cellular wall, this method sporoderm-broken rate is lower, pollen needs could handle after soaking into colloid, complicated operation, and the pollen behind the breaking cellular wall also needs dry if will make powder.
3) comminution by gas stream: be to utilize the compressed air pump air to produce high pressure draught, spray into pulverizing chamber with velocity of sound,, reach the purpose of pulverizing pollen at low temperatures by collision through nozzle.This method operation is complicated, and the pollen grain inclusions behind the breaking cellular wall is exposed in the external environment fully, is easy to generate oxidation deterioration.
4) fermentation enzymolysis breaking cellular wall method: be a kind of application method more widely, the enzyme that utilizes the abundant microorganism of pollen self or produce by microbe inoculation, make the fovilla degraded, osmotic pressure raises and makes pollen broken wall, required time of this method breaking cellular wall is long, and is easily influenced product quality by living contaminants in the sweat.
The objective of the invention is to propose to adopt radiation pollen grain integral body not to be gone to pot and rapid breaking cellular wall, and the high a kind of process for breaking wall of pollen of breaking cellular wall efficient.
In order to achieve the above object, the technical solution used in the present invention is: the pollen of fresh or drying is packed in the container that radiation-resistant packaging material for food (as glass, polyethylene) makes, under room temperature, normal pressure, be placed on the radiation source that can be used for food irradiation, as
60C
OOr
137C
SDown, carry out radiation treatment, radiation dose is 1KGy～11KGy (kilogray), is preferably 5KGy～10KGy, and the radiation treatment time is 24 hours.Preferably use vacuum-packed technique,, can adopt the packing specification of 100g, 1kg, 20kg, 50kg to reduce radiation and Oxidation when storing.
The present invention compared with prior art, the useful effect that has is:
1, adopt radiation that the interior macromolecular substances (as protein, DNA etc.) of pollen cell is degraded, secondary structure is destroyed, infiltration voltage rise height in the cell; Radiation improves the Cytoplasm membrane permeability, and inclusions collapses expansion from pollen germination pore easily, the effect that reaches not only breaking cellular wall but also sterilize.
2,, help nutritional labeling and preserve because pollen grain integral body does not have destroyedly, and inclusions still is present in the pollen wall under drying condition.
3, easy and simple to handle, the efficient height, energy savings does not produce the refuse of contaminated environment, is highly suitable for industrialized mass.
Be embodiments of the invention below:
Embodiment 1: take by weighing exsiccant Pollen Maydis, Flos Chrysanthemi powder, Pollen Helianthi 10g respectively, in the vial of packing into, be placed on
60C
OUnder the radiation source, respectively through 3KGy, 5KGy, 7KGy, 9KGy, 11KGy radiation treatment, the radiation treatment time is 24 hours.Get each 1g of pollen sample of radiation treatment and matched group, add the 9ml distilled water diluting, observe, count, calculate sporoderm-broken rate and be listed as follows at the OLYMPUS microscopically:
The table 1 average sporoderm-broken rates of different pollen kinds (%)
| The pollen kind | Matched group | Radiation treatment dosage (KGy) | |||
| ????3 | ????5 | ????7 | ????9 | ||
| Corn | ??9.01 | ????16.69 | ????18.60 | ????21.53 | ????36.30 |
| Flos Chrysanthemi | ??23.7 | ????41.2 | ????51.1 | ????49.7 | ????53.7 |
| Helianthi | ??19.2 | ????41.1 | ????45.2 | ????51.3 | ????52.1 |
Matched group in table 2 his-and-hers watches 1 and result of the test are carried out variance analysis
Annotate: df: degree of freedom
| Kind | Source of variation | ????df | ????SS | ????MS | ????F | Significance |
| Corn | Between processing | ????5 | ????0.785 | ??0.157 | ????78.2 | ????** |
| In the processing | ????54 | ????0.097 | ??0.002 | |||
| Flos Chrysanthemi | Between processing | ????5 | ????0.89 | ??0.178 | ????59.33 | ????** |
| In the processing | ????54 | ????0.184 | ??0.003 | |||
| Helianthi | Between processing | ????5 | ????0.79 | ??0.158 | ????31.6 | ????** |
| In the processing | ????54 | ????0.269 | ??0.005 |
SS: sum of sguares of deviation from mean
MS: sample mean variance
F: mean square between processing to error average variance ratio
*: expression difference is extremely remarkable
Through variance analysis, find that radiation treatment is remarkable to the sporoderm-broken rate effect that improves pollen such as corn, Flos Chrysanthemi, Helianthi.
Embodiment 2: take by weighing dry Folium Camelliae sinensis pollen 10g, its wall-breaking method is identical with embodiment 1, and its sporoderm-broken rate is listed as follows:
The average sporoderm-broken rate of the different places of production of table 3 Flos Camelliae Japonicae powder (%)
| The place of production | Matched group | Radiation treatment dosage (KGy) | ||||
| ????3 | ????5 | ????7 | ????9 | ????11 | ||
| Linan, Zhejiang | ??20.5 | ??85.7 | ??95.9 | ??98.2 | ??95.8 | ??98.5 |
| Yixing, Jiangsu | ??9.0 | ??88.11 | ??95.2 | ??98.3 | ??98.1 | ??98.1 |
| Chunan, Zhejiang | ??38.4 | ??82.6 | ??86.6 | ??94.6 | ??97.2 | ??96.9 |
| Anhui | ??39.8 | ??93.6 | ??95.1 | ??95.0 | ??97.1 | ??95.7 |
| The Tonglu, Zhejiang | ??57.7 | ??80.6 | ??90.4 | ??94.4 | ??94.7 | ??96.9 |
Table 4 his-and-hers watches 3 matched groups and test group result carry out variance analysis
| The place of production | Source of variation | ????df | ????SS | ????MS | ????F | Significance |
| Linan, Zhejiang | Between processing | ????5 | ??2.355 | ??0.471 | ????471 | ????** |
| In the processing | ????24 | ??0.019 | ??0.001 | |||
| Yixing, Jiangsu | Between processing | ????5 | ??3.160 | ??0.632 | ????632 | ????** |
| In the processing | ????24 | ??0.028 | ??0.001 | |||
| Chunan, Zhejiang | Between processing | ????5 | ??1.226 | ??0.253 | ????84.33 | ????** |
| In the processing | ????24 | ??0.074 | ??0.003 | |||
| Anhui | Between processing | ????5 | ??1.287 | ??0.037 | ????18.5 | ????** |
| In the processing | ????24 | ??0.059 | ??0.002 | |||
| The Tonglu, Zhejiang | Between processing | ????5 | ??0.556 | ??0.111 | ????37 | ????** |
| In the processing | ????24 | ??0.075 | ??0.003 |
Through variance analysis, find that radiation treatment is remarkable to the sporoderm-broken rate effect that improves Flos Camelliae Japonicae powder.
The expert group of coordination committee mechanism of World Health Organization (WHO) reaches a conclusion recently, can not bring any toxicologic threat to the radiating food of the radiation dose of 10KGy, and the food of handling with this dosage no longer needs any toxicologic evaluation.
Claims (3)
1. process for breaking wall of pollen, it is characterized in that: the pollen of fresh or drying is packed in the container that radiation-resistant packaging material for food makes, under room temperature, normal pressure, be placed under the radiation source that can be used for food irradiation, carry out radiation treatment, radiation dose is 1KGy～11KGy, and the radiation treatment time is 24 hours.
2. process for breaking wall of pollen according to claim 1, it is characterized in that: radiation dose is preferably 5KGy～10KGy.
3. process for breaking wall of pollen according to claim 1 and 2 is characterized in that: the radiation source that is used for food irradiation is
60C
OOr
137C
S
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00105084A CN1101148C (en) | 2000-04-25 | 2000-04-25 | Process for breaking wall of pollen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00105084A CN1101148C (en) | 2000-04-25 | 2000-04-25 | Process for breaking wall of pollen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1273784A true CN1273784A (en) | 2000-11-22 |
| CN1101148C CN1101148C (en) | 2003-02-12 |
Family
ID=4577518
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN00105084A Expired - Fee Related CN1101148C (en) | 2000-04-25 | 2000-04-25 | Process for breaking wall of pollen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1101148C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103125800A (en) * | 2013-03-11 | 2013-06-05 | 中国林业科学研究院亚热带林业研究所 | Processing technology of pollen pini for preventing and controlling Indian meal moth damage |
| CN112121965A (en) * | 2020-09-07 | 2020-12-25 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1026167C (en) * | 1987-09-03 | 1994-10-12 | 浙江省农业科学院 | Preparation process of the pollen essence |
-
2000
- 2000-04-25 CN CN00105084A patent/CN1101148C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103125800A (en) * | 2013-03-11 | 2013-06-05 | 中国林业科学研究院亚热带林业研究所 | Processing technology of pollen pini for preventing and controlling Indian meal moth damage |
| CN112121965A (en) * | 2020-09-07 | 2020-12-25 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
| CN112121965B (en) * | 2020-09-07 | 2021-12-10 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1101148C (en) | 2003-02-12 |
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| C06 | Publication | ||
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| C14 | Grant of patent or utility model | ||
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Granted publication date: 20030212 Termination date: 20100425 |