CN1027854C - Technology of breaking pollen wall - Google Patents

Abstract

The present invention relates to a pollen wall cracking technique which has the technical essential that two times of sterilization are adopted during wall cracking, and helicase is adopted to carry out enzyme digestion to make surface active substances of pollen spores agglomerate and make the active substances easy to absorb. Simultaneously, pollen walls are made thinner and to crack. The pollen is embrittled through snap freezing at-31 to-35 DEG C. After dilation, the pollen is pulverized with a low temperature pulverizer. Accordingly, the effective nutrient components can be furthest extracted. The present invention has simple technological method, and dispenses with special equipment. The product of the present invention is in accordance with national pertinent rules. The content of various kinds of amino acid is higher than that of similar products of American 1<#> and 2<#> by 4 to 10 times. Furthermore, the present invention maintains the characteristics of color, taste and perfume of the original pollen. The present invention is particularly suitable for the production of various kinds of pollen.

Description

Technology of breaking pollen wall

The present invention relates to a kind of pollen wall breaking technology, mainly be to be raw material with the natural spp. pollen that contains the abundant nutrition composition, comprise processing steps such as pollen purification and impurity removal, enzymeization, cold short, broken wall, and then extract the pollen wall-breaking technology method of its active ingredient to greatest extent.

Well-known natural spp. pollen is a kind of 18 seed amino acids, protein, multivitamin of containing, 20 several kinds of mineral elements, wherein contain zinc, iron, calcium, sodium, potassium, phosphorus of higher needed by human etc., also contain high nutrients such as plurality of enzymes and coenzyme class, bioactivator such as lipase, phosphatase, amylase, carbohydrase.Therefore pollen is as medical-care health food and existing very long history, just on the books in Shennong's Herbal, enter the fifties in this century, there are many countries such as Japan in the world, the U.S., countries such as Sweden have carried out the commodity research and development, substantially understood fully the nutritional labeling in the pollen, yet the contained nutriment of melissa powder but is included in the tough and tensile two-layer outer wall, can be removed the key of coming to greatest extent and be whether advanced science of wall-breaking method, therefore, in recent years, all paying attention to the broken shell Study on Technology both at home and abroad, as external enzymatic isolation method, because the complex enzyme that adopts, the biological active matter of pollen there is certain destruction, thereby the trophism in the reduction pollen, yet its wall but there is not abated effect; The disclosed temperature differential method of Japanese Patent Publication 54-3106 is to annotate 100 ℃ of hot water from-20 ℃ of freezing backs vitamin is damaged, adopt the cryogenic temperature of-10 ℃ and 0 ℃～-30 ℃ in and for example domestic 85109099A pollen shell breaking method and the 85106076A pollen wall breaking technology respectively, because the cryogenic temperature that said method adopts, do not reach the congealing point of pollen itself as yet, do not cause sporoderm-broken rate not ideal enough so reach " embrittlement "; What also have uses mechanical crushing method, makes its nutritional labeling suffer to destroy in various degree owing to temperature is high.In addition in the pollen wall breaking technology in the past, usually adopt the process of finished product sterilization, be suitable inadequately, because melissa powder from the nature collection, its surface very easily is stained with all kinds of pathogenic bacteriums, and these contaminated bacterias should be sterilized before broken wall and are advisable, otherwise its finished product is an inedibility, does not also meet national food hygienic standard simultaneously.

The object of the present invention is to provide that a kind of process is simple, sporoderm-broken rate is high, meet national food hygienic standard, can extract the pollen wall breaking technology of its effective nutritional labeling to greatest extent.

Technical essential of the present invention is to sterilize, adopt glusulase to carry out enzymeization before broken wall to make the cohesion of pollen spore surface reactive material be easy to absorb, make the pollen wall attenuation simultaneously and break, carry out quick-frozen embrittlement, expansion back low temperature mechanical breaking-wall method again, reach the sporoderm-broken rate height, fully extract the purpose of effective nutritional labeling, its technical process is:

1, pollen is sterilized;

2, with the aqueous solution of the glusulase pollen after to sterilization, 15～20 ℃ temperature, carry out enzymeization in airtight 1～2 hour;

3, the pollen after the enzymeization is sent in-31～-35 ℃ of medical refrigerators and carried out quick-frozen 20 hours, placed 10 ± 2 ℃ of insulating boxs then five hours;

4, dry in 42 ± 1 ℃ of drying bakers of immigration;

5, pulverize with the Lowtemperaturepulverizer broken wall that is lower than 42 ℃.

Described sterilization is with 75% alcoholic solution of pollen weight 3%, sprays rear enclosed while stirring one hour, and the edible white vinegar with pollen weight 2% carried out under the same terms sterilizing the second time with the first time again.

The described glusulase aqueous solution is the aqueous solution of 1～2 ‰ glusulase.

The pollen of handling with the inventive method proves that through the amino acid content check and analysis its all kinds of amino acid contents all exceed 4～10 times than the like product of U.S. 1#, 2#, thereby illustrate that its sporoderm-broken rate height, vitamin are not damaged, the recovery rate height of biological active matter in the pollen, its product meets the provisions of the relevant regulations issued by the State, the characteristics such as look, flavor, perfume (or spice) that kept propollen, simultaneously technology simple, need not special equipment and implement to be beneficial to easily to apply.

With most preferred embodiment, be described in detail the concrete technology contents of the inventive method below:

1, selected qualified pollen is 100 kilograms, with the quick rinsing decontamination of distilled water silt, makes its ash content be lower than 3%.

2, stir while spraying with 3 kilogram 75% alcoholic solution, seal and carried out the sterilization first time in one hour, use 2 kilograms of edible white vinegars and similarity condition for the first time again, carry out second time and sterilize.

3, the aqueous solution with 1 ‰ glusulase waters on the pollen after the sterilization under 15～20 ℃ of temperature, seals and carries out the enzyme processing in 1～2 hour.

4, the good pollen of enzymeization is sent in-31～-35 ℃ the refrigerator carried out quick-frozen 20 hours, placed then in 10 ± 1 ℃ the insulating box five hours.

5, put into the good pollen of enzymeization airtight and have 42 ± 1 ℃ of steam vent infrared drying baker oven dry.

6, carry out pulverize and break cellular wall with the low temperature roller pulverizer that is lower than 42 ℃ and cross 100 mesh sieves.

7, will sieve down pollen is that carrier is made scattered paste shape with 5% cyclodextrin and 2% albumen sugar, goes in 42 ± 1 ℃ of infrared drying bakers baking after stirring and falls part moisture content, makes wet-milling, makes diameter 30 micron particles with granulator.

8, send Co after the packing ⁶⁰X-ray room X sterilizes at last.

Embodiment 2(makes the Normal juice pollen of not being with pollen wall) its technical process is as follows:

1, selected qualified pollen is 100 kilograms, with the quick rinsing decontamination of distilled water silt, makes its ash content be lower than 3%.

2, with 2 kilogram 75% alcoholic solution spray stir after, seal two hours, the pollen that purifies is carried out sterilization first time, again with 2 kilograms of edible white vinegars and the first time the same terms carry out the second time and sterilize.

3, the pollen after the sterilization carry out enzymeization with 2 ‰ the glusulase aqueous solution.

4, send in-35 ℃ the refrigerator and carried out quick-frozen 20 hours.

5, dry down through negative pressure-500 millimetres of mercury again.

6, pulverized 100 mesh sieves with the Lowtemperaturepulverizer that is lower than 42 ℃.

7, dissolve with 5 times distilled water or 75% alcohol pollen crushing screening.

8, separate pollen wall with centrifuge, make pollen Normal juice, be condensed into liquid pollen.

9, the pollen particles packing of making protective clothing is sent Co ⁶⁰The chamber sterilization.

Above-mentioned technical process was operated in twice halfhour workshop of ultraviolet radiation sterilization in every day, the pollen of producing with the technology of the present invention has and extracts effective nutritional labeling to greatest extent, meet the provisions of the relevant regulations issued by the State, ash is lower than 3%, and look, flavor, the fragrant characteristics of maintenance propollen, free from extraneous odour easily is the high-grade nutrient food that human body absorbed, both food, food additives, beauty food can be done, medicine, various drinks raw material, cosmetics can be done again.

Claims (1)

1, a kind of process for breaking wall of pollen mainly is to be that raw material comprises pollen purification and impurity removal, enzymeization, cold short, technology for broken wall step with the natural spp. pollen that contains the abundant nutrition composition, it is characterized in that:

1) pollen behind the purifying and dedusting being sterilized, is 75% alcoholic solution with pollen weight 3%, sprays rear enclosed one hour while stirring, and the edible white vinegar with pollen weight 2% carried out under the same terms sterilizing the second time with the first time again;

2) with the aqueous solution of the 1-2 ‰ glusulase pollen after to sterilization, 15～20 ℃ temperature, carry out enzymeization in airtight 1～2 hour;

3) pollen after the enzymeization is sent in-31～-35 ℃ of refrigerators and carried out quick-frozen 20 hours, placed 10 ± 2 ℃ of insulating boxs then five hours;

4) dry in 42 ± 1 ℃ of drying bakers of immigration;

5) pulverize with the Lowtemperaturepulverizer broken wall that is lower than 42 ℃.