CN114798124A - Preparation device and method of spore water aqua based on laser irradiation non-oxidation wall breaking technology - Google Patents
Preparation device and method of spore water aqua based on laser irradiation non-oxidation wall breaking technology Download PDFInfo
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C19/00—Other disintegrating devices or methods
- B02C19/18—Use of auxiliary physical effects, e.g. ultrasonics, irradiation, for disintegrating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C23/00—Auxiliary methods or auxiliary devices or accessories specially adapted for crushing or disintegrating not provided for in preceding groups or not specially adapted to apparatus covered by a single preceding group
Abstract
The invention discloses a preparation device and a preparation method of a spore water aqua based on a laser irradiation non-oxidation wall breaking technology, which comprises the steps of stirring and wall breaking, wherein in a container cup flow field, through stirring a suspension material which accords with Newtonian fluid at a proper speed, the effect that the inertia force is larger than the viscous force is realized, so that the turbulent flow is realized in the flow field in the container cup, namely, solid granular spores are displaced as fast as possible to move on the outermost layer of a cylindrical rotary water aqua and can be turned up and down; greatly reduces the laser irradiation burning time required by wall breaking of medicinal plant spores and pollen spores, and solves the problem of light pollution caused by long-time irradiation burning which can be caused by directly using laser light energy to wall breaking of the plant spores, namely the destructive influence of the heat effect and the light effect on nutrient substances of the spore contents during laser irradiation burning.
Description
Technical Field
The invention belongs to the technical field of biotechnology, and particularly relates to a preparation device and a preparation method of a spore water agent based on a laser irradiation non-oxidation wall breaking technology.
Background
It is known that the medicinal plant spores and pollen spores are the essence and nutrition treasury of plants, and the spore content contains all active nutritional ingredients required by human life bodies and many nutritional ingredients which can not be synthesized by human at present, wherein the nutritional ingredients comprise various proteins, various vitamins, amino acids, mineral substances, enzymes and coenzymes, nucleic acid, monosaccharide, triterpene, polysaccharide and the like, and the total content is more than 200. The plant spores in nature are various in variety and different in efficacy. For example, the rape pollen spores have good efficacy in treating male prostate diseases. More than 20 amino acids such as glutamic acid, proline, etc. contained in rape pollen can improve the blood circulation of prostate tissue and relieve urethral obstruction caused by prostatic hyperplasia, and flavonoids have strong antioxidant function, can prevent prostatic hyperplasia and prostate dysfunction, has the treatment rate of prostatitis of 90 percent, and is also a killer for prostatic hyperplasia.
The plant spore wall breaking technology has been developed for more than twenty years, so far, the extraction of spore contents without oxidation, damage and pollution is still a technical problem, so that no national standard and industrial standard appear so far, because the previous spore wall breaking technology comprises a chemical method; a biological enzyme method; mechanical methods; physical methods and the like have such disadvantages. For example, physical methods currently prevailing in the industry include temperature difference wall breaking; ultrasonic wall breaking method; wind tunnel (high pressure) supersonic airflow wall breaking method; ultra-low temperature air flow impact wall breaking methods and the like, the physical wall breaking equipment has large one-time investment, complex equipment structure and high production cost, is difficult to be used for large-scale production, is difficult to control the wall breaking rate and is polluted by heavy metal, and the volatilization loss phenomenon is serious and the recovery is difficult because spore content is nano-scale free substances after wall breaking.
The laser wall-breaking spore technology can not only obtain nutrient substances of inclusions in spores, but also solve the problems of nutrient volatilization loss and pollution of the inclusions in the spores after wall breaking. However, in the process of breaking spore wall by laser, the main difficulties are: the passing speed of the spore passing through the wall-breaking burning area of the laser pulse facula (focus) can meet the requirement that the spore obtains wall-breaking light energy and can prevent the spore inclusion from being damaged, burned, carbonized, gasified and the like caused by the heat effect of laser over burning. Secondly, to prevent the destructive effects of stimulation, inhibition, decomposition and the like on spore contents caused by the light effect of laser burning for too long time, the time for the spores to receive laser irradiation needs to be as short as possible.
And the stirring method in the container in the prior industry comprises the following steps: mechanical stirring, air-flow stirring, jet stirring, etc., and is suitable for only mechanical stirring in a small right-circular cylindrical vessel cup by using the light energy of laser. The mechanical stirring method is characterized in that except for a magnetic stirring device, more classical stirring devices comprise five types and various modifications thereof, wherein the five types of mechanical stirring devices comprise: stirring in a paddle manner; stirring in a turbine mode; thirdly, propeller type stirring; anchor stirring; stirring in a spiral belt type.
The five types of stirring devices are not suitable for the technical field of laser wall-broken spores, and the stirring devices aim to solve the stirring of liquid-liquid mixing, gas-liquid mixing and solid-liquid mixing in a container, namely stirring for mixing, and gas, liquid and suspended particles in a solution are uniformly mixed by stirring and circulating. The stirring technology of the spore wall-broken by the laser is not to uniformly mix, but to make all the ultrafine solid particle spores of suspension fluid in a container efficiently pass through the irradiation cauterization action area of laser spots in a turbulent flow form, which is essentially different from other stirring methods.
Disclosure of Invention
In order to solve the problems in the prior art, the invention aims to provide a device and a method for preparing a spore water aqua based on a laser irradiation non-oxidation wall breaking technology.
The technical scheme adopted by the invention is as follows:
the preparation method of the spore water agent based on the laser irradiation non-oxidation wall breaking technology comprises the following steps:
s1, stirring; in a flow field of the container cup, the suspension material conforming to Newtonian fluid is stirred at a proper speed, so that the influence of inertia force is greater than viscous force, the flow field in the container cup realizes turbulent flow, namely solid particle spores are displaced to the outermost layer of the cylindrical rotary water aqua as fast as possible and can be turned up and down;
s2, breaking the wall; the force applied to the solid particles is proportional to the mass of the particles, and spores with large mass can quickly occupy the outermost layer of the fluid (i.e. the inner wall of the container cup). When the spores spirally rise to pass through a set laser spot area, the spores are burned and broken to cause the escape of the inclusions and lose the complete quality, the escaped inclusions are instantly fused with water, the spore wall shells after wall breaking are in an incomplete and irregular block shape and lose the original quality, the spores which are not burned by the spots occupy the outermost layer of fluid because the spores do not lose the quality, the spores continue to flow forward through stirring and then repeatedly do the flow of spiral rising and falling, and the state is repeated until all the spores in the material container are almost burned by the irradiation of the spots to break the walls.
Preferably, in step S1, the right cylindrical container cup and the wading stirring member made of medical grade material are selected so that the micron-sized solid particles repeatedly flow along the inner wall of the material cup from bottom to top and spirally rise and fall around the inner wall of the cylindrical container.
In step S1, the present invention preferably uses two-layer paddle blades for stirring, the upper layer uses flat paddles for radial stirring, and the lower layer uses inclined paddles for axial stirring.
The preparation device of the spore water agent based on the laser irradiation non-oxidation wall breaking technology comprises a laser, a three-dimensional displacement material platform, a rotary tray, a stirring mechanism, a container cup and a foam recovery tank;
preferably, the laser is a nanosecond laser and/or a picosecond laser, and the light-emitting combination parameter and the scanning parameter of the laser are determined by the main component of the spore wall shell and the layer number of the spore wall shell.
Preferably, the three-dimensional displacement material table is used for positioning light spots emitted by matching with the laser;
the rotating tray is driven to rotate by a variable speed direct current motor, the rotating speed control range is 0-50 rpm, and the rotating tray is used for controlling the container cup to rotate.
Preferably, the stirring mechanism consists of a micro stepless speed change cantilever type direct current motor and a transmission part, the stirring rotating speed is controlled within the range of 0-1500 rpm, and a wading part of the stirring mechanism is a polytetrafluoroethylene coating part and/or a glass material part.
Preferably, the container cup is used for containing materials, and is made of a transparent and high-temperature-resistant material;
the container cup is in a right cylinder shape, one end of the container cup is provided with an opening, the end, provided with the opening, of the container cup is detachably provided with a container cover, a bearing seat is fixedly arranged on the container cover, a rotating shaft is rotatably arranged on the bearing seat and penetrates through the container cover, a transmission shaft is detachably arranged at one end, located outside the container cup, of the rotating shaft, and a flat pulp and an inclined pulp are fixedly arranged at one end, located inside the container cup, of the rotating shaft; the connection part of the rotating shaft and the container cover is sealed by a silica gel sealing shaft sleeve.
Preferably, the container cover is made of metal, the inner side of the container cover is coated with polytetrafluoroethylene, the container cover is further provided with a through hole, the through hole is connected with an exhaust pipe, and the exhaust pipe is made of silica gel.
Preferably, the foam recovery tank is made of food-grade PP and/or silica gel, the foam recovery tank is connected with the exhaust pipe, and the foam recovery tank is used for collecting foam and crushed slag pushed from the exhaust pipe.
The invention has the beneficial effects that:
1. greatly reduces the laser irradiation burning time required by wall breaking of medicinal plant spores and pollen spores, and solves the problem of light pollution caused by long-time irradiation burning which can be caused by directly using laser light energy to wall breaking of the plant spores, namely the destructive influence of the heat effect and the light effect on nutrient substances of the spore contents during laser irradiation burning.
2. The laser wall breaking is characterized in that the laser focusing energy is utilized, the content immediately enters water after the spore wall shell is burned and broken, and the material in the form of suspension is not very sensitive to the laser, so that the possibility of coking the content of the spore in the form of suspension is eliminated. According to the test results, the spore's contents will be attracted away by "van der Waals forces" as long as the spore has been burned by the laser to create a hole or a crack.
3. Almost has no possibility of volatilization loss of nutrient components of spore contents, the product from the production line is the wall-broken spore stock solution provided for downstream enterprises, and the downstream enterprises can carry out post-treatment such as wall-broken spore stock solution wall-shell separation, medical purification and the like according to own needs. The compound can be widely applied to the production of solid preparations such as granules, capsules, tablets, films and the like by downstream enterprises, even can be prepared into traditional Chinese medicine injections, and can also be widely applied to the health food industry and the beauty and skin care industry.
4. Has certain bactericidal effect. The invention has no phenomena of heavy metal pollution, chemical pollution, light pollution and nutrient loss of the inclusion, but has the function of killing most of bacteria because of the laser light energy.
5. The preparation device can be regarded as a station in the production line control system and a subordinate unit of the production line control system, one station on the production line is in fault, the production line can only withdraw the station from the production line without influencing the production of other stations, and the continuous production of the production line is ensured.
Drawings
The invention is described in further detail below with reference to the accompanying drawings and specific embodiments.
FIG. 1 is a schematic view of a container cup of the present invention;
FIG. 2 is a schematic of a laser profile of the present invention;
FIG. 3 is an electron microscope picture of wall-broken Ganoderma lucidum spore using the present invention;
FIG. 4 is an electron microscope picture of the wall-broken Ganoderma lucidum spore prepared by the method.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.
The following describes a specific embodiment of the present invention with reference to fig. 1-4, and the method for preparing the spore aqueous solution based on the laser irradiation non-oxidation wall-breaking technology comprises the following steps:
s1, stirring; in a flow field of the container cup, the suspension material conforming to Newtonian fluid is stirred at a proper speed, so that the influence of inertia force is greater than viscous force, the flow field in the container cup realizes turbulent flow, namely solid particle spores are displaced to the outermost layer of the cylindrical rotary water aqua as fast as possible and can be turned up and down;
s2, breaking the wall; the force applied to the solid particles is proportional to the mass of the particles, and spores with large mass can quickly occupy the outermost layer of the fluid (i.e. the inner wall of the container cup). When the spores spirally rise to pass through a set laser spot area, the spores are burned and broken to cause the escape of the inclusions and lose the complete quality, the escaped inclusions are instantly fused with water, the spore wall shells after wall breaking are in an incomplete and irregular block shape and lose the original quality, the spores which are not burned by the spots occupy the outermost layer of fluid because the spores do not lose the quality, the spores continue to flow forward through stirring and then repeatedly do the flow of spiral rising and falling, and the state is repeated until all the spores in the material container are almost burned by the irradiation of the spots to break the walls.
Advantageously, in step S1, the right cylindrical container cup and the wading stirring member of medical grade material are selected such that the micron-sized solid particles repeatedly flow along the inner wall of the material cup from bottom to top and spirally up and back down around the inner wall of the cylindrical container.
Advantageously, in step S1, two layers of paddle blades are used for stirring, the upper layer is flat paddle for radial force stirring, and the lower layer is inclined paddle for axial force stirring.
The preparation device of the spore water agent based on the laser irradiation non-oxidation wall breaking technology comprises a laser 18, a three-dimensional displacement material platform, a rotary tray, a stirring mechanism, a container cup 11 and a foam recovery tank;
advantageously, the laser 18 is a nanosecond laser and/or a picosecond laser, the light output combination and scanning parameters of which are determined by the main component of the exine shell and the number of layers of the exine shell.
Beneficially, the three-dimensional displacement material stage is used to coordinate with spot positioning of the light emitted by the laser 18; the rotating tray is driven to rotate by a variable speed direct current motor, the rotating speed control range is 0-50 rpm, and the rotating tray is used for controlling the container cup 11 to rotate.
The stirring mechanism is composed of a micro stepless speed change cantilever type direct current motor and a transmission part, the stirring rotating speed is controlled within the range of 0-1500 rpm, and a wading part of the stirring mechanism is a polytetrafluoroethylene coating part and/or a glass material part.
Beneficially, the container cup 11 is used for containing materials, and the container cup 11 is made of light-transmitting and high-temperature-resistant materials; the container cup 11 is in a right cylinder shape, one end of the container cup 11 is provided with an opening, one end of the container cup 11 with the opening is detachably provided with a container cover 14, a bearing seat 12 is fixedly arranged on the container cover 14, a rotating shaft 13 is rotatably arranged on the bearing seat 12, the rotating shaft 13 penetrates through the container cover 14, one end of the rotating shaft 13, which is positioned outside the container cup 11, is detachably provided with a transmission shaft, and one end of the rotating shaft 13, which is positioned inside the container cup 11, is fixedly provided with a flat pulp 16 and an inclined pulp 17; the connection part of the rotating shaft 13 and the container cover 14 is sealed by a silica gel sealing shaft sleeve.
Beneficially, the container cover 14 is made of metal, the inner side of the container cover 14 is coated with polytetrafluoroethylene, and the container cover 14 is further provided with a through hole, the through hole is connected with an exhaust pipe, and the exhaust pipe is made of silica gel.
Advantageously, the foam recovery tank is made of food-grade PP and/or silica gel, the foam recovery tank is connected with the exhaust pipe, and the foam recovery tank is used for collecting foam and crushed slag pushed from the exhaust pipe.
The use method of the invention comprises the following steps:
the material is in a solid-liquid suspension form and has stability, and when the external condition is not changed, even if the material is stirred or stands for a long time, spores cannot be dissolved in water;
because spores are micron-sized solid particles (the sizes of medicinal plant spores and pollen spores are generally 6-60 um), the spores are heavier than water and are insoluble in water, the materials are in a suspension liquid form when being stirred in a container, and after standing for a period of time, spore particles are deposited at the bottom of a cup and have an obvious clear layered interface with water;
when the external conditions of laser wall breaking are changed, the content of the spore is instantly dissolved in water at the wall breaking moment, after standing for a period of time, the spore wall shell is deposited at the bottom of the container cup, and the fusion of the content of the spore and the water leads the material to be in a turbid liquid state;
and the mass percentage ratio concentration of the ultrafine solid particle spores and the water aqua of the material should maintain the form of Newtonian fluid, so that the influence of the spores on the fluid viscosity is reduced as much as possible. Newtonian fluids refer to low viscosity fluids in which the shear stress of the fluid is proportional to the rate of deformation after a force is applied.
The process comprises the following steps: the flow of the fluid is divided into three basic forms of laminar flow, transitional flow and turbulent flow, and in a right-cylinder-shaped container cup flow field, the influence of inertia force is larger than viscous force by stirring turbid liquid materials which accord with Newtonian fluid at a proper speed, so that the flow field in the container cup can realize a special form of turbulent flow as much as possible, namely solid particle spores are displaced to the outermost layer of a cylindrical rotary water aqua as fast as possible and can be turned up and down.
And selecting a wading stirring component which is suitable for medical grade or food grade safe materials and a suitable stirring method, so that the micron-sized solid particles flow repeatedly along the inner wall of the material cup from bottom to top and spirally rise and fall around the inner wall of the cylindrical container. The force applied to the solid particles is proportional to the mass of the particles, and spores with large mass can quickly occupy the outermost layer of the fluid (i.e. the inner wall of the container cup). As the spores spiral up through the set laser spot area, the spores are burned and broken causing the contents to escape losing their intact mass, their escaping contents instantaneously fusing with water. After wall breaking, spore shells are in an incomplete and irregular block shape and lose the original quality, spores which are not burned by light spots and wall breaking occupy the outermost layer of fluid because the quality is not lost, the spores continue to flow forward by stirring and then repeatedly do spiral rising and falling flow, and the state is repeated until all the spores in the material container are almost burned by light spot irradiation to break the walls.
The turbulence is the most important problem which is not solved by the classical physics, and the turbulent flow field in the container cup has no mature calculation formula in the aspect of stirring so far, so that the turbulence can be verified only by a large number of tests. For example, stirring with a two-layer paddle blade: calculating and testing the power distribution problem of an axial force stirring inclined paddle type blade (always arranged on the lower layer) and a radial force flat paddle type stirring blade (always arranged on the upper layer) according to different types of spores (so that the sizes and specific gravities of the spores) and different proportioning concentrations of the spores and water in the material, namely calculating and testing the respective paddle diameter sizes of the two layers of blades and the spacing distance between the two layers of paddle type blades so as to realize the optimal flow field shape in the container cup, namely supplying the axial force to the fluid by the lower blades; the upper vanes provide radial force to the fluid. The axial direction is the common direction with the mandrel in the container cup, the radial direction is perpendicular to the axial direction, and the radial direction is perpendicular to the axial space. Stirring the spores from the bottom into a shape of rolling up and falling back along the inner wall of the material container cup by using axial force; making the spores to rotate and flow in the radius direction of the end face circle of the material container cup by utilizing radial force; the combined force of the axial force and the radial force is utilized to lead the spores to do spiral rising flow and fall back along the inner wall of the material container, thereby reducing the central collapse of the liquid level. Or a special stirring rod is adopted to enable the container cup and the stirring rod to form a thin interlayer, and the materials are rotated in the thin interlayer to be burnt by laser irradiation.
And calculating and testing a proper proportion of the stirring part to the inner diameter of the container cup according to different types of plant spores and different proportioning concentrations of the spores and water in the material, so that enough gaps are reserved on the inner wall of the container cup to allow liquid to flow in an up-and-down circular manner, otherwise, convection circulation of suspension fluid is hindered, and the laser irradiation burning time is prolonged. Meanwhile, to prevent the generation of upper and lower two convection cycles, part of spores in the lower cycle cannot receive laser irradiation burning.
The preparation device comprises: the laser is fixed in place on a work table. The multi-laser irradiation cauterization can be carried out on one container cup, but if the multi-laser irradiation is adopted, a three-dimensional displacement base needs to be added to a laser base participating in the multi-laser irradiation so as to accurately position the light spot. Meanwhile, attention is paid to the light emitting direction, namely the placement angle, of each laser, and direct opposite irradiation is avoided, so that the lasers are prevented from being damaged (shown in figure 2).
And secondly, measuring and calculating the approximate position of the light spot distance according to the size of the focusing lens of each type of laser, and fixing a manual/electric control three-dimensional displacement material table on the workbench according to the light emitting requirement of the first laser (without a three-dimensional displacement base).
Thirdly, fixing the container cup rotating tray on the manual/electric control three-dimensional displacement material table, and mainly paying attention to the fact that the center position of the light emitting lens of the laser is basically aligned with the center position of the container cup in the height direction. If magnetic stirring is adopted, the center of the rotating tray of the container cup is designed to be hollow so as to install a rotating magnetic disk mechanism, and a motor for driving the rotating magnetic disk is connected to a remote magnetic stirring controller through a connecting wire.
The center of the container cup on the container cup rotating tray can be located by a physical method or an optical method, so that the container cup can be guaranteed to be not eccentric all the time by the rotating of the container cup rotating tray during working.
The silica gel container cup cover can be used for magnetic stirring, and the exhaust pipe penetrates through the container cup cover to be connected with the recovery tank. The metal cup covers which need to use polytetrafluoroethylene coatings, such as various blade stirring or various shaft lever stirring, are adopted, the shaft lever for driving the stirring penetrates through the cup cover of the container to be connected with the bearing seat and the transmission mechanism, the transmission shaft lever on the cup cover is plugged by a silica gel sealing shaft sleeve through a hole, and the exhaust pipe penetrates through the cup cover of the container to be connected with the recovery tank.
Assembly line: the prepared uniformly dispersed suspension materials are filled into the container cups through a quantitative filling machine in a preparation room, for example, the container cups are 500ml, the filling materials are 300ml, and a space for turning up the materials when the container cups are stirred is reserved.
The container cups which are filled with the materials are placed at a fixed position on a conveyor belt and conveyed into an oxygen-free channel through the conveyor belt.
The anaerobic channel is a closed small workshop, and the laser wall breaking processing is completed in the anaerobic channel. The interior of the oxygen-free channel is dust-free, oxygen-free and air-free and is filled with nitrogen.
If an electric control three-dimensional displacement material platform is adopted, the laser can be remotely controlled to emit light at a light spot position, and a manipulator can be used for replacing the container cup. If a manual mode is adopted, staff who enter an anaerobic channel need to be protected from oxygen deficiency when the material cup is replaced.
The recovery method of the container cup stock solution for completing the wall breaking comprises the step of conveying the finished product to a closed sub-packaging chamber through a finished product conveying belt, wherein the closed sub-packaging chamber can be designed to be a chamber in which operators complete sub-packaging operation through extending-in silica gel gloves.
And (4) after the finished product stock solution is packed into the closed transfer barrel in the oxygen-free closed sub-packaging chamber, carrying the closed transfer barrel to a finished product warehouse.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and the like are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be directly connected or indirectly connected through an intermediate member, or they may be connected through two or more elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The foregoing is merely exemplary and illustrative of the present invention and various modifications, additions and substitutions may be made by those skilled in the art to the specific embodiments described without departing from the scope of the invention as defined in the following claims.
Claims (10)
1. The preparation method of the spore water agent based on the laser irradiation non-oxidation wall breaking technology is characterized by comprising the following steps:
s1, stirring; in a flow field of the container cup, the suspension material conforming to Newtonian fluid is stirred at a proper speed, so that the influence of inertia force is greater than viscous force, the flow field in the container cup realizes turbulent flow, namely solid particle spores are displaced to the outermost layer of the cylindrical rotary water aqua as fast as possible and can be turned up and down;
s2, breaking the wall; the stress of solid particles is in direct proportion to the mass of particles, spores with large mass can occupy the outermost layer of fluid (namely the inner wall of a container cup) quickly, when the spores rise spirally through a set laser spot area, the spores are burned and broken to cause the escape of inclusions and lose the complete mass, the escaped inclusions are instantly fused with water, spore wall shells after wall breaking are incomplete, irregular broken blocks and lose the original mass, the spores which are not burned by the light spots and have wall breaking are occupied the outermost layer of fluid because the spores do not lose the mass, spiral rising and falling flow are continuously and repeatedly carried out by stirring until all the spores in the material container are burned by light spot irradiation and have wall breaking.
2. The preparation method of the spore water aqua based on the laser irradiation non-oxidation wall breaking technology according to claim 1, which is characterized in that: in step S1, a right cylindrical container cup and a wading stirring member made of a medical grade material are selected so that the micron-sized solid particles repeatedly flow along the inner wall of the material cup from bottom to top while spirally rising and falling around the inner wall of the cylindrical container.
3. The preparation method of the spore water aqua based on the laser irradiation non-oxidation wall breaking technology according to claim 1, which is characterized in that: two layers of paddle type blades are adopted for stirring, the upper layer adopts a flat paddle for stirring by radial force, and the lower layer adopts an inclined paddle for stirring by axial force.
4. The device for preparing the spore aqueous solution based on the laser irradiation non-oxidation wall-breaking technology provides the method for preparing the spore aqueous solution based on the laser irradiation non-oxidation wall-breaking technology, which is characterized in that: comprises a laser, a three-dimensional displacement material platform, a rotary tray, a stirring mechanism, a container cup and a foam recovery tank.
5. The device for preparing the spore water aqua based on the laser irradiation non-oxidation wall breaking technology according to claim 4, is characterized in that: the laser is a nanosecond laser and/or a picosecond laser, and the light-emitting combination parameter and the scanning parameter of the laser are determined by the main components of the spore exine and the layer number of the exine.
6. The device for preparing the spore water aqua based on the laser irradiation non-oxidation wall breaking technology according to claim 4, is characterized in that: the three-dimensional displacement material table is used for positioning light spots emitted by the laser in a matching manner; the rotating tray is driven to rotate by a variable speed direct current motor, the rotating speed control range is 0-50 rpm, and the rotating tray is used for controlling the container cup to rotate.
7. The device for preparing the spore water aqua based on the laser irradiation non-oxidation wall breaking technology according to claim 4, is characterized in that: the stirring mechanism consists of a micro stepless speed change cantilever type direct current motor and a transmission part, the stirring rotating speed is controlled within the range of 0-1500 rpm, and a wading part of the stirring mechanism is a polytetrafluoroethylene coating part and/or a glass material part.
8. The device for preparing the spore water aqua based on the laser irradiation non-oxidation wall breaking technology according to claim 4, is characterized in that: the container cup is used for containing materials and is made of a transparent and high-temperature-resistant material; the container cup is in a right cylinder shape, one end of the container cup is provided with an opening, the end, provided with the opening, of the container cup is detachably provided with a container cover, a bearing seat is fixedly arranged on the container cover, a rotating shaft is rotatably arranged on the bearing seat and penetrates through the container cover, a transmission shaft is detachably arranged at one end, located outside the container cup, of the rotating shaft, and a flat pulp and an inclined pulp are fixedly arranged at one end, located inside the container cup, of the rotating shaft; the connection part of the rotating shaft and the container cover is sealed by a silica gel sealing shaft sleeve.
9. The device for preparing the spore water aqua based on the laser irradiation non-oxidation wall breaking technology according to claim 8, is characterized in that: the container lid is the metal material, the container lid inboard is polytetrafluoroethylene by the coating, still be equipped with the through-hole on the container lid, the through-hole is connected with the blast pipe, the blast pipe adopts the silica gel material.
10. The device for preparing the spore water aqua based on the laser irradiation non-oxidation wall breaking technology according to claim 9, is characterized in that: the foam recovery tank is made of food-grade PP and/or silica gel, the foam recovery tank is connected with the exhaust pipe, and the foam recovery tank is used for collecting foam and crushed slag pushed by the exhaust pipe.
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6440420B1 (en) * | 2001-03-19 | 2002-08-27 | Xin Liu | Method for extracting oleaginous substances from germination-activated Ganoderma lucidum spores |
| EP1504809A1 (en) * | 2003-08-08 | 2005-02-09 | ETH Zürich | Rotating stirring device with substantially narrow distribution of energy dissipation rate |
| KR20110136316A (en) * | 2010-06-14 | 2011-12-21 | 삼성테크윈 주식회사 | Method and apparatus for manufacturing high-purity nano colloid |
| CN206897526U (en) * | 2017-04-17 | 2018-01-19 | 长沙环境保护职业技术学院 | A kind of microbe in solid state culture medium sterile crushing device |
| CN209753030U (en) * | 2019-04-11 | 2019-12-10 | 史丹利化肥扶余有限公司 | High-speed shearing machine for producing fourth element high-tower compound fertilizer |
| CN111019834A (en) * | 2020-01-14 | 2020-04-17 | 龚霖迪 | Differential pressure wall breaking method for ganoderma lucidum spore powder for inhibiting tumors and resisting aging |
| CN111903961A (en) * | 2020-09-07 | 2020-11-10 | 宁燕平 | Preparation method of spore water aqua based on laser irradiation non-oxidation wall breaking technology |
| CN212040800U (en) * | 2020-03-25 | 2020-12-01 | 烟台寿蔬野果生物科技有限公司 | Cherry plum prune accords with high-efficient agitating unit of magma |
| CN112121965A (en) * | 2020-09-07 | 2020-12-25 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
| CN113634180A (en) * | 2021-09-03 | 2021-11-12 | 湖南源科工程技术有限公司 | Equipment that laser plant spore nonoxidation broken wall material preparation was used |
| CN215611093U (en) * | 2021-09-03 | 2022-01-25 | 湖南源科工程技术有限公司 | Turbulence generator for breaking wall of laser spore |
| CN215625422U (en) * | 2021-09-03 | 2022-01-25 | 湖南源科工程技术有限公司 | Material feeding device for preparing spore and pure water turbid liquid |
-
2022
- 2022-03-18 CN CN202210272767.4A patent/CN114798124A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6440420B1 (en) * | 2001-03-19 | 2002-08-27 | Xin Liu | Method for extracting oleaginous substances from germination-activated Ganoderma lucidum spores |
| EP1504809A1 (en) * | 2003-08-08 | 2005-02-09 | ETH Zürich | Rotating stirring device with substantially narrow distribution of energy dissipation rate |
| KR20110136316A (en) * | 2010-06-14 | 2011-12-21 | 삼성테크윈 주식회사 | Method and apparatus for manufacturing high-purity nano colloid |
| CN206897526U (en) * | 2017-04-17 | 2018-01-19 | 长沙环境保护职业技术学院 | A kind of microbe in solid state culture medium sterile crushing device |
| CN209753030U (en) * | 2019-04-11 | 2019-12-10 | 史丹利化肥扶余有限公司 | High-speed shearing machine for producing fourth element high-tower compound fertilizer |
| CN111019834A (en) * | 2020-01-14 | 2020-04-17 | 龚霖迪 | Differential pressure wall breaking method for ganoderma lucidum spore powder for inhibiting tumors and resisting aging |
| CN212040800U (en) * | 2020-03-25 | 2020-12-01 | 烟台寿蔬野果生物科技有限公司 | Cherry plum prune accords with high-efficient agitating unit of magma |
| CN111903961A (en) * | 2020-09-07 | 2020-11-10 | 宁燕平 | Preparation method of spore water aqua based on laser irradiation non-oxidation wall breaking technology |
| CN112121965A (en) * | 2020-09-07 | 2020-12-25 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
| CN113634180A (en) * | 2021-09-03 | 2021-11-12 | 湖南源科工程技术有限公司 | Equipment that laser plant spore nonoxidation broken wall material preparation was used |
| CN215611093U (en) * | 2021-09-03 | 2022-01-25 | 湖南源科工程技术有限公司 | Turbulence generator for breaking wall of laser spore |
| CN215625422U (en) * | 2021-09-03 | 2022-01-25 | 湖南源科工程技术有限公司 | Material feeding device for preparing spore and pure water turbid liquid |
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