CN107245371A - A kind of method of utilization impulse electric field assisted extraction microalgae grease - Google Patents
A kind of method of utilization impulse electric field assisted extraction microalgae grease Download PDFInfo
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- CN107245371A CN107245371A CN201710358924.2A CN201710358924A CN107245371A CN 107245371 A CN107245371 A CN 107245371A CN 201710358924 A CN201710358924 A CN 201710358924A CN 107245371 A CN107245371 A CN 107245371A
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- electric field
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- impulse electric
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- broken wall
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of method of utilization impulse electric field assisted extraction microalgae grease, including following steps：A：Broken wall step, is pumped into impulse electric field by certain density microalgae algae solution and carries out broken wall treatment；B：Algae solution carries out centrifugal treating after centrifugation step, the broken wall obtained to step A；C：Extraction step, takes algal gel and addition Extraction solvent progress extraction reaction thereto that step B is obtained, extraction time is 0.5 30 h；D：Distilled water is added in extraction step, the product obtained to step C and organic phase containing grease is obtained, organic solvent volatilization is treated totally, crude oil fat is obtained after drying.Impulse electric field broken wall microalgae cell has broken wall efficiency high, cost low, advantage capable of being industrialized；Meanwhile, the higher chloroform/methanol of traditional toxicity is replaced using low poison solvent, while extraction efficiency is ensured extraction process can be made more environmentally-friendly, a kind of technology with good industrial prospect is provided for microalgae grease.
Description
Technical field
Field, especially a kind of method of utilization impulse electric field assisted extraction microalgae grease are extracted the invention belongs to grease.
Background technology
Using microalgae as raw material prepare biodiesel technology by microalgae algae kind seed selection, the culture of microalgae, microalgae receipts
Obtain, grease extract and thick fat several key links compositions such as transesterification.The higher cost that wherein grease is extracted is microalgae diesel oil
One of main bottleneck of process industry.The cost that wet method carries fat is relatively low, but lacks microalgae wall breaking technology capable of being industrialized at present
And the low poison solvent of chloroform methanol can be substituted.Domestic and international researcher is ground to the various methods of microalgae cell broken wall
Study carefully, including multigelation method, bead mill method, high pressure homogenization method, ultrasonic method, microwave method in Mechanical Method, acid-base method in chemical method,
Osmotic shock method and bioanalysis etc., but there is industrialization bottleneck in economic or technology in these methods.Therefore in the urgent need to seeking
Look for low a kind of broken wall efficiency high, cost, green low toxicity and the microalgae grease extractive technique with preferable industrial prospect.
Pulse electric field technology (PulsedElectricFields, PEF) be with higher electric-field intensity (10-50kV/cm),
Shorter pulse width（0-100μs）Higher pulse frequency（0-2000Hz）Liquid, semi-solid food products are handled, and
And the production line of continuous sterilizing and sterile filling can be constituted.
Mainly there are following two effect collaborations to carry out.
1st, impulse electric field produces magnetic field, and this impulse electric field and pulsed magnetic field alternating action increases cell leakage, shaken
Aggravation is swung, film-strength weakens, thus film is destroyed, and material is easily flowed out in film, and outer membrane material is easily infiltrated into, the protection of cell membrane
Declines even disappear.
2nd, living matter is acted in the anions and canons and film that the material ionization nearby of ionization electrode is produced, thus is blocked
The progress of normal biochemical reaction and metabolic processes etc. in film；Meanwhile, the ozoniferous strong oxidation of liquid medium ionization production
Effect, can occur series reaction with intracellular matter.
Impulse electric field method（PEF）A kind of new nom-thermal sterilization technology in food processing field, with its treatment temperature it is low, when
Between good application characteristic the causes domestic and international researcher such as short, energy consumption is low, pollution is small extensive concern, industrial prospect ten
Divide wide.So far, PEF technologies are widely used in the various aspects in food engineering field, including the sterilizing of food, dry it is de-
Water, material extraction etc..Therefore consider that PEF technologies are applied into microalgae grease extracts preparation field of biodiesel oil.
For this reason, it is necessary to designing a kind of new microalgae grease extracts method, extraction grease that can efficiently, inexpensive.
The content of the invention
The main object of the present invention is to provide a kind of method of utilization impulse electric field assisted extraction microalgae grease, for overcoming
Existing Microalgae grease extraction method cost is high, the problem of be difficult to industrial applications.
The present invention is achieved in that a kind of method of utilization impulse electric field assisted extraction microalgae grease, including following step
Suddenly：
Step A：Certain density microalgae algae solution is pumped into impulse electric field and carried out at broken wall by broken wall step, the broken wall step system
Reason；
Step B：Algae solution carries out centrifugal treating after centrifugation step, the broken wall that the centrifugation step system obtains to step A；
Step C：Extraction step, the extraction step system takes the algal gel of step B acquisitions and adds Extraction solvent thereto and carried
Negate and answer, extraction time is 0.5-30 h；
Step D：Distilled water is added in extraction step, the product that the extraction step system obtains to step C and is obtained has containing grease
Machine phase, treats organic solvent volatilization totally, crude oil fat is obtained after drying.By this method can quickly, low cost to microalgae oil
Fat is extracted.
The present invention further technical scheme be：The microalgae be tamed by mutagenesis chlorella, grid algae, Closterium,
One or more in Dunaliella salina algae kind.This method is applied widely.
The present invention further technical scheme be：Microalgae concentration is 1 × 10 when broken wall treatment is carried out in step A7-1×108
Individual/mL, electric-field intensity is that 5-50 kV/cm, frequency are that 10 kHz-200 kHz, pulsewidth are that 2-20 μ s, sample pass through pulse electricity
Flow velocity be that 3-15 L/h, cycle-index are 1-5 times.Broken wall efficiency when above-mentioned parameter is to broken wall plays decisive work
With.The broken wall efficiency of microalgae refers to accounting of the dead cell in total microalgae cell number after broken wall.The data can pass through reality
Test is obtained.Impulse electric field is made up of high-voltage pulse power source, oscillograph, process chamber, the part of computerized control system four, the pulse electricity
Field parameters refer to flow velocity by impulse electric field of the electric-field intensity, frequency, pulsewidth, sample of set impulse electric field, circulation
The parameters such as number of times.
The present invention further technical scheme be：Centrifugation rate is 5000-8000 r/min in step B, and centrifugation time is
5-10 min。
The present invention further technical scheme be：Extract solution used is n-hexane/isopropanol, n-hexane/second in step C
One kind in alcohol, n-hexane/normal propyl alcohol, petroleum ether/ethanol, ethyl acetate/methanol, ethyl acetate/ethanol compound system.Use
Above-mentioned composite solvent system replaces existing frequently-used chloroform/methanol, and chloroform/methanol toxicity is stronger, and above-mentioned compound system base
This toxicity is weaker.
The present invention further technical scheme be：The first solution and second solution in extract solution in the step C
Match as volume ratio 3:1、2:1、3:2、1:1、2:3 and 1:One kind in 2.
The present invention further technical scheme be：Extract solution and the volume ratio that preceding algae solution is centrifuged in step B are 1 in step C:
3 、1:2、1:1、2:1、3:It is a kind of in 1.
The present invention further technical scheme be：Extract reaction in step C to carry out on shaking table, the rotating speed of shaking table is 100-
200 r/min。
The present invention further technical scheme be：The volume of the amount and the amount of product in step C of distilled water is added in step D
Than for 1:7、1:5、1:It is a kind of in 3；The drying temperature of organic layer is 73-82 DEG C.
The present invention further technical scheme be：Product in step C is layered in step D by centrifugation, centrifugal rotational speed is
5000-8000 r/min, centrifugation time are 3-10 min.
The beneficial effects of the invention are as follows：This programme constructs a kind of side of utilization impulse electric field assisted extraction microalgae grease
Method, impulse electric field broken wall microalgae cell has broken wall efficiency high, cost low, advantage capable of being industrialized；Meanwhile, using low poison solvent
Instead of the higher chloroform/methanol of traditional toxicity, while extraction efficiency is ensured extraction process can be made more environmentally-friendly, be micro-
Algae oil fat, which is extracted, provides a kind of technology with good industrial prospect.
Brief description of the drawings
, below will be to embodiment or existing in order to illustrate more clearly of the embodiment of the present application or technical scheme of the prior art
There is the accompanying drawing used required in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments described in application, for those of ordinary skill in the art, on the premise of not paying creative work,
Other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is the schematic diagram of the method for utilization impulse electric field assisted extraction microalgae grease provided in an embodiment of the present invention.
Embodiment
The present invention provides a kind of method of utilization impulse electric field assisted extraction microalgae grease.Below in conjunction with drawings and Examples
The present invention is described in detail.
As shown in figure 1, the technical scheme is that：Build a kind of side of utilization impulse electric field assisted extraction microalgae grease
Method, including 1, impulse electric field processing system；2nd, microalgae algae solution；3rd, pump；4th, the microalgae algae solution after impulse electric field broken wall；5th, algae solution from
The heart simultaneously adds grease Extraction solvent into algal gel after centrifugation；6th, extraction reaction is carried out on shaking table, after reaction terminates, is added a certain amount of
Distilled water centrifugation layering, draw organic phase containing grease, treat organic solvent volatilization totally, after drying acquisition crude oil fat.
Specific implementation process is as follows：Finite concentration microalgae algae solution 2 is entered in impulse electric field processing system 1 by peristaltic pump 3
Process chamber, set impulse electric field parameter carry out the microalgae algae solution 4 after impulse electric field broken wall treatment, gained broken wall through centrifugation at
Reason, Extraction solvent 5 is added into centrifugation gained algal gel, is placed on shaking table 6 and is carried out extraction reaction, after reaction terminates, adds
A certain amount of distilled water centrifugation layering, draws organic phase containing grease, treats organic solvent volatilization totally, is obtained after being dried in baking oven
Crude oil fat.In specific embodiment, the microalgae is the algae kinds such as chlorella, grid algae, Closterium, the Dunaliella salina tamed by mutagenesis
In one or more.In specific embodiment, during impulse electric field processing microalgae, microalgae concentration is 1 × 107-1×108Individual/mL, electricity
Field intensity is that 5-50 kV/cm, frequency are that 10 kHz-200kHz, pulsewidth are that the flow velocity that 2-20 μ s, sample pass through impulse electric field is
3-15 L/h, cycle-index are 1-5 times.In specific embodiment, the grease Extraction solvent be n-hexane/isopropanol, n-hexane/
Match somebody with somebody between one kind in ethanol, n-hexane/normal propyl alcohol, petroleum ether/ethanol, ethyl acetate/methanol and ethyl acetate/ethanol, solvent
Than for 3:1、2:1、3:2、1:1、2:3 and 1:One in 2 six levels, solvent and algae solution ratio（Liquid ratio）For 1:3 、1:2、
1:1、2:1、3:One in 1 five levels, extraction time is 0.5-30 h.In specific embodiment, algal gel obtained by the centrifugation,
Centrifugation rate is 5000-8000 r/min, and the time is 5-10 min.The shaking speed is 100-200 r/min.It is described from
The heart is layered, and centrifugation rate is 5000-8000 r/min, and the time is 3-10 min.It is described to add a certain amount of distilled water centrifugation point
Layer, the ratio of added distillation water consumption and solvent for use is 1:7、1:5、1:One in 3 three levels.
With reference to specific embodiment, the more excellent case study on implementation of the present invention is described in further detail.
Embodiment 1
（1）It is determined that there is the parameter significantly affected on chlorella cell wall disruption：Impulse electric field is determined by dead cell counts method living
Electric-field intensity, frequency, pulsewidth, sample are influence impulse electric fields to chlorella cell wall disruption by the flow velocity of impulse electric field, cycle-index
The key factor of effect；
（2）Obtain the optimum condition that impulse electric field auxiliary microalgae grease is extracted：By changing impulse electric field gain of parameter chlorella
（Concentration is 1.2 × 107Individual/mL）Grease extraction efficiency, when drawing chlorella grease extraction efficiency highest, the parameter of impulse electric field is field
Strong 20 kV/cm, the L/h of flow velocity 5.4, the Hz of frequency 130, the μ s of pulsewidth 6, cycle-index 3 times.
（3）Obtain nontoxic or low toxicity chlorella grease Extraction solvent：By grease extraction efficiency, from n-hexane/isopropanol,
Filtered out just in n-hexane/ethanol, n-hexane/normal propyl alcohol, petroleum ether/ethanol, ethyl acetate/methanol and ethyl acetate/ethanol
Hexane/ethanol is the optimum solvent for replacing chloroform/methanol.
（4）Solvent dosing method optimizes：By grease extraction efficiency, optimization preferably solvent dosing method is 60 ml algaes
After liquid centrifuges 10 min through 8000 r/min, the ml of n-hexane 36, the ml of ethanol 24 are added in algal gel, is horizontally placed on 150 on shaking table
Rpm shakes 3 h, and reaction adds 12ml distilled water thereto after terminating, 8000 r/min centrifugations 5min makes layering, draws and contain grease
N-hexane phase, treat organic solvent volatilization totally, 80 DEG C drying after obtain crude oil fat.
Embodiment 2
（1）It is determined that there is the parameter significantly affected on chlorella cell wall disruption：Impulse electric field is determined by dead cell counts method living
Electric-field intensity, frequency, pulsewidth, sample are influence impulse electric fields to chlorella cell wall disruption by the flow velocity of impulse electric field, cycle-index
The key factor of effect；
（2）Obtain the optimum condition that impulse electric field auxiliary microalgae grease is extracted：By changing impulse electric field gain of parameter chlorella
（Concentration is 1.6 × 107Individual/mL）Grease extraction efficiency, when drawing chlorella grease extraction efficiency highest, the parameter of impulse electric field is field
Strong 20 kV/cm, the L/h of flow velocity 4.2, the Hz of frequency 150, the μ s of pulsewidth 10, cycle-index 3 times.
（3）Obtain nontoxic or low toxicity chlorella grease Extraction solvent：By grease extraction efficiency, from n-hexane/isopropanol,
Filtered out just in n-hexane/ethanol, n-hexane/normal propyl alcohol, petroleum ether/ethanol, ethyl acetate/methanol and ethyl acetate/ethanol
Hexane/ethanol is the optimum solvent for replacing chloroform/methanol.
（4）Solvent dosing method optimizes：By grease extraction efficiency, optimization preferably solvent dosing method is 40 ml algaes
After liquid centrifuges 10 min through 8000 r/min, the ml of n-hexane 24, the ml of ethanol 16 are added in algal gel, is horizontally placed on 150 on shaking table
Rpm shakes 3 h, and reaction adds 8ml distilled water thereto after terminating, 8000 r/min centrifugations 5min makes layering, draws and contain grease
N-hexane phase, treat organic solvent volatilization totally, 80 DEG C drying after obtain crude oil fat.
Embodiment 3
（1）It is determined that there is the parameter significantly affected on chlorella cell wall disruption：Impulse electric field is determined by dead cell counts method living
Electric-field intensity, frequency, pulsewidth, sample are influence impulse electric fields to chlorella cell wall disruption by the flow velocity of impulse electric field, cycle-index
The key factor of effect；
（2）Obtain the optimum condition that impulse electric field auxiliary microalgae grease is extracted：By changing impulse electric field gain of parameter chlorella
（Concentration is 2.3 × 107Individual/mL）Grease extraction efficiency, when drawing chlorella grease extraction efficiency highest, the parameter of impulse electric field is field
Strong 20 kV/cm, the L/h of flow velocity 4.2, the Hz of frequency 150, the μ s of pulsewidth 10, cycle-index 3 times.
（3）Obtain nontoxic or low toxicity chlorella grease Extraction solvent：By grease extraction efficiency, from n-hexane/isopropanol,
Filtered out just in n-hexane/ethanol, n-hexane/normal propyl alcohol, petroleum ether/ethanol, ethyl acetate/methanol and ethyl acetate/ethanol
Hexane/ethanol is the optimum solvent for replacing chloroform/methanol.
（4）Solvent dosing method optimizes：By grease extraction efficiency, optimization preferably solvent dosing method is 30 ml algaes
After liquid centrifuges 10 min through 8000 r/min, the ml of n-hexane 18, the ml of ethanol 12 are added in algal gel, is horizontally placed on 150 on shaking table
Rpm shakes 3 h, and reaction adds 6ml distilled water thereto after terminating, 8000 r/min centrifugations 5min makes layering, draws and contain grease
N-hexane phase, treat organic solvent volatilization totally, 80 DEG C drying after obtain crude oil fat.
This programme constructs a kind of method of utilization impulse electric field assisted extraction microalgae grease, and impulse electric field broken wall microalgae is thin
Born of the same parents have broken wall efficiency high, cost low, advantage capable of being industrialized；Meanwhile, replace traditional toxicity higher using low poison solvent
Chloroform/methanol, can make extraction process more environmentally-friendly, one kind is provided for microalgae grease while extraction efficiency is ensured
Technology with good industrial prospect.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
Claims (10)
1. a kind of method of utilization impulse electric field assisted extraction microalgae grease, it is characterised in that comprise the following steps：
Step A：Certain density microalgae algae solution is pumped into impulse electric field and carried out at broken wall by broken wall step, the broken wall step system
Reason；
Step B：Algae solution carries out centrifugal treating after centrifugation step, the broken wall that the centrifugation step system obtains to step A；
Step C：Extraction step, the extraction step system takes the algal gel of step B acquisitions and adds Extraction solvent thereto and carried
Negate and answer, extraction time is 0.5-30 h；
Step D：Distilled water is added in extraction step, the product that the extraction step system obtains to step C and is obtained has containing grease
Machine phase, treats organic solvent volatilization totally, crude oil fat is obtained after drying.
2. the method for utilization impulse electric field assisted extraction microalgae grease according to claim 1, it is characterised in that：It is described micro-
Algae be tamed by mutagenesis chlorella, grid algae, Closterium, the one or more in Dunaliella salina algae kind.
3. the method for utilization impulse electric field assisted extraction microalgae grease according to claim 1, it is characterised in that：Step A
Microalgae concentration is 1 × 10 during middle progress broken wall treatment7-1×108Individual/mL, electric-field intensity is that 5-50 kV/cm, frequency are 10
KHz-200 kHz, pulsewidth are that the flow velocity that 2-20 μ s, sample pass through impulse electric field is that 3-15 L/h, cycle-index are 1-5 times.
4. the method for utilization impulse electric field assisted extraction microalgae grease according to claim 1, it is characterised in that：Step B
Middle centrifugation rate is 5000-8000 r/min, and centrifugation time is 5-10 min.
5. the method for utilization impulse electric field assisted extraction microalgae grease according to claim 1, it is characterised in that：Step C
In extract solution used be n-hexane/isopropanol, n-hexane/ethanol, n-hexane/normal propyl alcohol, petroleum ether/ethanol, ethyl acetate/first
One kind in alcohol, ethyl acetate/ethanol compound system.
6. the method for utilization impulse electric field assisted extraction microalgae grease according to claim 5, it is characterised in that：The step
The proportioning of the first solution and second of solution is volume ratio 3 in extract solution in rapid C:1、2:1、3:2、1:1、2:3 and 1:In 2
It is a kind of.
7. the method for utilization impulse electric field assisted extraction microalgae grease according to claim 6, it is characterised in that：Step C
Middle extract solution is 1 with the volume ratio that preceding algae solution is centrifuged in step B:3 、1:2、1:1、2:1、3:It is a kind of in 1.
8. the method for utilization impulse electric field assisted extraction microalgae grease according to claim 7, it is characterised in that：Step C
Middle to extract reaction progress on shaking table, the rotating speed of shaking table is 100-200 r/min.
9. the method for utilization impulse electric field assisted extraction microalgae grease according to claim 1, it is characterised in that：Step D
The volume ratio of the middle amount for adding distilled water and the amount of product in step C is 1:7、1:5、1:It is a kind of in 3；The drying temperature of organic layer
For 73-82 DEG C.
10. the method for utilization impulse electric field assisted extraction microalgae grease according to claim 9, it is characterised in that：Step D
In be layered in step C product by centrifugation, centrifugal rotational speed is that 5000-8000 r/min, centrifugation time are 3-10 min.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107794115A (en) * | 2017-12-06 | 2018-03-13 | 哈尔滨工业大学深圳研究生院 | A kind of method that broken wall assisted extraction microalgae grease is digested using protozoal predation |
| CN108587917A (en) * | 2018-05-25 | 2018-09-28 | 宋庆恒 | Chlorella pyrenoidosa cell utilizes the method that manioc waste is that primary raw material prepares biodiesel |
| CN108925957A (en) * | 2018-08-28 | 2018-12-04 | 湖南狄师傅食品科技有限公司 | A kind of organic hot pepper essential oil and pungent snack |
| CN111329054A (en) * | 2020-03-10 | 2020-06-26 | 广东健微营养科技有限公司 | A nutritional preparation containing Haematococcus pluvialis and its preparation method |
| CN111961525A (en) * | 2020-09-07 | 2020-11-20 | 中科广化（重庆）新材料研究院有限公司 | Method for extracting acer truncatum fruit edible oil |
| CN112121965A (en) * | 2020-09-07 | 2020-12-25 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
| CN113769437A (en) * | 2021-10-22 | 2021-12-10 | 武汉轻工大学 | Method for rapidly extracting high-quality bioactive components from microalgae cells and application thereof |
| WO2022036967A1 (en) * | 2020-08-20 | 2022-02-24 | 中国海洋大学 | Pulse current generator and control method |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107794115A (en) * | 2017-12-06 | 2018-03-13 | 哈尔滨工业大学深圳研究生院 | A kind of method that broken wall assisted extraction microalgae grease is digested using protozoal predation |
| CN108587917A (en) * | 2018-05-25 | 2018-09-28 | 宋庆恒 | Chlorella pyrenoidosa cell utilizes the method that manioc waste is that primary raw material prepares biodiesel |
| CN108587917B (en) * | 2018-05-25 | 2020-12-29 | 杭州富阳高博信息技术服务有限公司 | Method for preparing biodiesel by using chlorella pyrenoidosa cells as main raw material by using cassava residues |
| CN108925957A (en) * | 2018-08-28 | 2018-12-04 | 湖南狄师傅食品科技有限公司 | A kind of organic hot pepper essential oil and pungent snack |
| CN108925957B (en) * | 2018-08-28 | 2021-07-27 | 湖南狄师傅食品科技有限公司 | Organic pepper essential oil and spicy snack |
| CN111329054A (en) * | 2020-03-10 | 2020-06-26 | 广东健微营养科技有限公司 | A nutritional preparation containing Haematococcus pluvialis and its preparation method |
| WO2022036967A1 (en) * | 2020-08-20 | 2022-02-24 | 中国海洋大学 | Pulse current generator and control method |
| CN111961525A (en) * | 2020-09-07 | 2020-11-20 | 中科广化（重庆）新材料研究院有限公司 | Method for extracting acer truncatum fruit edible oil |
| CN112121965A (en) * | 2020-09-07 | 2020-12-25 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
| CN112121965B (en) * | 2020-09-07 | 2021-12-10 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
| CN113769437A (en) * | 2021-10-22 | 2021-12-10 | 武汉轻工大学 | Method for rapidly extracting high-quality bioactive components from microalgae cells and application thereof |
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