CN108938681A - It is a kind of to have both the antitumor soft wall-breaking method of lucidum spore powder enzyme fermentation of anti-aging anticancer - Google Patents
It is a kind of to have both the antitumor soft wall-breaking method of lucidum spore powder enzyme fermentation of anti-aging anticancer Download PDFInfo
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- CN108938681A CN108938681A CN201810825216.XA CN201810825216A CN108938681A CN 108938681 A CN108938681 A CN 108938681A CN 201810825216 A CN201810825216 A CN 201810825216A CN 108938681 A CN108938681 A CN 108938681A
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- powder
- lucidum spore
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- spore powder
- aging
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Abstract
The antitumor soft wall-breaking method of lucidum spore powder enzyme fermentation of anti-aging anticancer is had both the present invention provides a kind of, utilize the parental strain of liquid spawn cultivating method culture, it induces lucidum spore powder natural decomposition and softens broken wall, the present invention carries out broken wall decomposition to conidia powder by fermentation by the enzyme of ganoderma lucidum parental strain itself, sporoderm-broken rate is up to 99.99%, it not only can thoroughly solve microorganism decomposition enzyme bacterium residue problem, stationary culture broken wall, keep the integrality of spore, spore is met water and is just decomposed, solve laser method, chemical method, conidia powder holding time short problem after the broken walls such as physical method, substantially increase shelf lives.The effective ingredient of the tough and tensile outer wall of conidia powder is available simultaneously is absorbed and utilized; the polyoses content of conidia powder is apparently higher than the polyoses content of other ganoderma spove powders of nature; protective rate has ensured the generation of secondary destruction during conidia powder effective component is not processed 99.17%.
Description
Technical field
The present invention designs the soft broken wall field of lucidum spore powder enzyme fermentation, and in particular to one kind has both anti-aging and antitumor work
The soft wall breaking technology of lucidum spore powder enzyme fermentation.
Background technique
In lucidum spore powder a variety of pharmacological activity such as polysaccharide, alkaloid, organic germanium and terpenoid rich at
Part, the immunity function of human body can be significantly improved, promotes the generation of IgM and IgC, there are the functions such as antiviral, anti-canceration, inhibiting cancer.
It is an elongated silk thread under an optical microscope, it is line of demarcation that it has cell wall between external environment；In cell wall
Inside contain cytoplasm, nucleus, the structures such as vacuole, mitochondria and plasma membrane.But the cell wall of conidia powder is tougher, dry weight
About the 30% of cell total weight.Its main function is to maintain the shape of cell, the organelle for protecting it internal.This layer of outer wall object
Matter is not soluble in water, is also insoluble in the chitin of acid, and after spore enters stomach, effective component can not be absorbed by the body utilizations, influence
The performance of its effect.Meanwhile this layer of outer wall is rich in s- glucan abundant, chitin, protein and β-(1 → 4) key connection
N- acerylglucosamine (- 2 deoxidations of 2- acetamide-D-glucose) composition, wherein chitin and acerylglucosamine
Content composition accounting highest, chitin have the effects that activating cell, inhibit aging, prevention disease, adjust human body physiological function,
The ground six big vital principle one of arranged side by side with protein, fat, carbohydrate, vitamin, minerals is described as by American-European academia.Acetyl
Base gucosamine, which has, inhibits excessive growth of cancer cells or fibrocytes, plays inhibition to cancer and malignant tumor and treatment is made
With.And traditional wall-breaking method is only handled by the way of breaking outer wall, it can not be by people after tough and tensile outer wall is edible
Body absorbs.Therefore, the protection technique of effective component has become Reishi sporule new drug after dynamical breaking trachytectum technology and broken wall
An important factor for development and utilization.The common method of the technical field has at present:
1. chemical broken wall: since chemical method broken wall mechanism is by external force break up cell wall, so broken cell wall is broken
Piece remains in cell liquid.
2. physical mechanical broken wall: sporoderm-broken rate is not high, and the spore after broken wall has metal impurities, and effective component is by serious
It destroys, it is not high as drug effect of raw material exploitation.
3. air-flow broken wall: energy consumption is high, to be generally higher by other methods several times, there is also crush the limit, grinding particle size for air-flow
Linear with yield, yield is bigger, and granularity is bigger.
4. biological enzyme broken wall: cell wall is dissolved in the presence of biology enzyme, is allowed to not exist, in contrast, enzyme process
More thoroughly, shell-broken effect is significantly better than other methods to broken wall ratio.The disadvantage is that require enzyme have high activity, the specificity of height and
Mild reaction condition.It is thin to additionally depend on the type of enzyme, dosage, enzymolysis time, temperature, pH value, material for shell-broken effect simultaneously
A variety of composite factors such as degree, stirring.Therefore, this method is more demanding to technological means and research and development ability.
Summary of the invention
A kind of high, effective component that the technical problem to be solved in the present invention is to provide sporoderm-broken rates minimum damage during broken wall
The wall breaking technology for losing, improving effective component absorptivity and guaranteeing the quality period length, it is of the existing technology to solve the problems, such as.
In order to solve the above-mentioned technical problem, The technical solution adopted by the invention is as follows:
A kind of to have both the antitumor soft wall-breaking method of lucidum spore powder enzyme fermentation of anti-aging anticancer, described has both anti-aging
The antitumor soft wall-breaking method of lucidum spore powder enzyme fermentation of anticancer includes the following steps:
(1) Reishi sporule for choosing fresh free from admixture, obtains Reishi sporule powder raw material.
(2) purified water is added to the Reishi sporule powder raw material in step 1 under stirring conditions, lucidum spore powder used is former
Material and the mass ratio of purified water are 1:2-10, are dispersed in Reishi sporule powder raw material in water；It stands, removes upper foam shape
Floating object discards precipitating；Intermediate fluid is filtered to remove impurity, obtains filtered fluid；
(3) filtered fluid in step 2 is subjected to filters pressing processing, obtains filter cake；Filter cake is crushed, is added and filter cake quality ratio
For the purified water of 10-1:1, it is uniformly mixed, obtains suspension；
(4) suspension in step 3 is subjected to vacuum freezedrying processing, obtains the lucidum spore powder of high-purity；
(5) lucidum spore powder of high-purity in step 4 is added in biological fermentation tank, and moisture is controlled 60%, and
It is irradiated by 60 cobalt-y and carries out sterilization treatment, access ganoderma lucidum parental strain；And into biofermentation in temperature environment being 25-28 DEG C
Tank is sealed by fermentation 20-25 days, so that conidia powder be made to obtain 100% broken wall；
(6) lucidum spore powder for taking out 100% broken wall in step (5), is put into dryer and carries out drying and sterilizing processing, dries
At 45-75 DEG C, drying time 1-3h, the conidia powder dried is in pellet shape, and is sealed in polybag for the temperature control of dry machine
Inside stored, it can complete entire technical process.
Further, in step (1), the Reishi sporule powder raw material is harvested on Korea S's ganoderma lucidum body of artificial cultivation.
Further, in step (4), the method for the vacuum freezedrying processing are as follows: by the suspension in step (3)
It is sub-packed in lyophilized plate, vacuumizes 1-60Pa, filling nitrogen to atmospheric pressure is cooled to 0 DEG C--35 DEG C in advance, is kept for 0.5-4 hours
Afterwards, 1-60Pa is vacuumized, heating sublimation is to 0-10 DEG C, then is cooled to 0-- 50 DEG C, and suspension is made to be frozen into ice cube solid, protects
It holds 1-15 hours；1-60Pa is vacuumized again, and heating is biochemical, and 15-30 as a child led to nitrogen to 10-80Pa, rises to temperature of charge
40 DEG C -6- DEG C, 2-16 hours are kept the temperature, discharging.
Further, qualification is extracted and preserved using liquid bacterial strain cultivation in ganoderma lucidum parental strain described in step (5)
Parental strain.
The utility model has the advantages that compared with prior art, the present invention has the advantage that
Beneficial effects of the present invention:
1) present invention utilizes the parental strain of the same germline of ganoderma lucidum fruitbody, induces Reishi sporule by the biological enzyme of itself
Powder softens broken wall naturally, and lucidum spore powder outer wall is made to obtain softening broken wall treatment.
2) the entire broken wall process of the present invention is not in contact with any other biological enzyme or other techniques, so will not remain any has
Evil substance and secondary pollution.
3) since there is no the outer wall complete structures that damage conidia powder kernel plays the role of anti-oxidation protection by the present invention, so
Conidia powder retention cycle after broken wall is longer than the conidia powder that other wall breaking technologies are processed.
4) outer wall of lucidum spore powder is rich in s- glucan abundant, chitin, protein and β-(1 → 4) key connection
N- acerylglucosamine (- 2 deoxidations of 2- acetamide-D-glucose) composition, wherein chitin and acerylglucosamine
Content composition accounting highest.Chitin has the effects that activating cell, inhibits aging, prevention disease, adjusts human body physiological function,
The ground six big vital principle one of arranged side by side with protein, fat, carbohydrate, vitamin, minerals is described as by American-European academia.N- second
Acyl glucose amine, which has, inhibits excessive growth of cancer cells or fibrocytes, plays inhibition and treatment to cancer and malignant tumor
Effect.Due to the tough and tensile outer wall that the outer wall of Reishi sporule has one layer of extremely difficult chitin for being digested by human body gastric acid to constitute, not broken wall
Or the conidia powder of other broken wall methods, human body are that can not digest and assimilate to the effective component of conidia powder whole or outer wall.By this hair
Human body can be improved 120% to the absorptivity of lucidum spore powder after bright softening broken wall, while by chitin and n- acetyl group Portugal
Grapes glucosamine has the intake of effective component to absorb, to play anti-aging and antitumor efficacy.
Specific embodiment
According to following embodiments, the present invention may be better understood.However, as it will be easily appreciated by one skilled in the art that real
It applies content described in example and is merely to illustrate the present invention, without sheet described in detail in claims should will not be limited
Invention.
Embodiment 1
A kind of to have both the antitumor soft wall-breaking method of lucidum spore powder enzyme fermentation of anti-aging anticancer, described has both anti-aging
The antitumor soft wall-breaking method of lucidum spore powder enzyme fermentation of anticancer includes the following steps:
(1) Reishi sporule for choosing fresh free from admixture, obtains Reishi sporule powder raw material.
(2) purified water is added to the Reishi sporule powder raw material in step 1 under stirring conditions, lucidum spore powder used is former
Material and the mass ratio of purified water are 1:2-10, are dispersed in Reishi sporule powder raw material in water；It stands, removes upper foam shape
Floating object discards precipitating；Intermediate fluid is filtered to remove impurity, obtains filtered fluid；
(3) filtered fluid in step 3 is subjected to filters pressing processing, obtains filter cake；Filter cake is crushed, is added and filter cake quality ratio
For the purified water of 10-1:1, it is uniformly mixed, obtains suspension；
(4) suspension in step 4 is subjected to vacuum freezedrying processing, obtains the lucidum spore powder of high-purity；
(5) lucidum spore powder of high-purity in step 4 is added in biological fermentation tank, and moisture is controlled 60%, and
It is irradiated by 60 cobalt-y and carries out sterilization treatment, access ganoderma lucidum parental strain；And into biofermentation in temperature environment being 25-28 DEG C
Tank is sealed by fermentation 20-25 days, so that conidia powder be made to obtain 100% broken wall；Wherein, ending standard is decomposed in culture, takes out sample,
It observes after adding water to conidia powder with 1600 times of high-power microscopes, up to subject to 99.99% broken wall, can not extend up to 99.99 broken walls such as
Incubation time, until up to subject to 99.99%；
(6) lucidum spore powder for taking out 100% broken wall in step (5), is put into dryer and carries out drying and sterilizing processing, dries
At 45-75 DEG C, drying time 1-3h, the conidia powder dried is in pellet shape, and is sealed in polybag for the temperature control of dry machine
Inside stored, it can complete entire technical process.
In the present embodiment, in step (1), the Reishi sporule powder raw material is harvested in Korea S's ganoderma lucidum body of artificial cultivation
On.
In the present embodiment, in step (4), the method for the vacuum freezedrying processing are as follows: by the suspension in step (3)
Liquid is sub-packed in lyophilized plate, vacuumizes 1-60Pa, and filling nitrogen to atmospheric pressure is cooled to 0 DEG C--35 DEG C in advance, is kept for 0.5-4 hours
Afterwards, 1-60Pa is vacuumized, heating sublimation is to 0-10 DEG C, then is cooled to 0-- 50 DEG C, and suspension is made to be frozen into ice cube solid, protects
It holds 1-15 hours；1-60Pa is vacuumized again, and heating is biochemical, and 15-30 as a child led to nitrogen to 10-80Pa, rises to temperature of charge
40 DEG C -6- DEG C, 2-16 hours are kept the temperature, discharging.
In the present embodiment, conjunction is extracted and preserved using liquid bacterial strain cultivation in ganoderma lucidum parental strain described in step (5)
The parental strain of lattice, 3%~6% specific gravity strain inoculation medium, with the bacterial strain newly grown up to, similarity condition is inoculated with again after growing up to
Culture medium, three times, with adding, the PDA culture medium slant medium of 3% specific gravity peptone is continuous to expand required amount of female parent tiltedly for inoculation altogether
Face bacterium.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (4)
1. a kind of have both the antitumor soft wall-breaking method of lucidum spore powder enzyme fermentation of anti-aging anticancer, which is characterized in that described
The antitumor soft wall-breaking method of lucidum spore powder enzyme fermentation of anti-aging anticancer is had both to include the following steps:
(1) Reishi sporule for choosing fresh free from admixture, obtains Reishi sporule powder raw material.
(2) purified water is added to the Reishi sporule powder raw material in step 1 under stirring conditions, Reishi sporule powder raw material used and
The mass ratio of purified water is 1:2-10, is dispersed in Reishi sporule powder raw material in water；It stands, it is floating to remove upper foam shape
Object discards precipitating；Intermediate fluid is filtered to remove impurity, obtains filtered fluid；
(3) filtered fluid in step 2 is subjected to filters pressing processing, obtains filter cake；Filter cake is crushed, addition is with filter cake quality ratio
The purified water of 10-1:1 is uniformly mixed, obtains suspension；
(4) suspension in step 3 is subjected to vacuum freezedrying processing, obtains the lucidum spore powder of high-purity；
(5) lucidum spore powder of high-purity in step 4 is added in biological fermentation tank, and moisture is controlled 60%, and is passed through
60 cobalt-y irradiation carries out sterilization treatment, accesses ganoderma lucidum parental strain；And temperature environment be 25-28 DEG C at it is close into biological fermentation tank
Seal ferment 20-25 days, so that conidia powder be made to obtain 100% broken wall；
(6) lucidum spore powder for taking out 100% broken wall in step (5), is put into dryer and carries out drying and sterilizing processing, dryer
Temperature control at 45-75 DEG C, drying time 1-3h, the conidia powder dried is in pellet shape, and be sealed in polybag it is interior into
Row storage, can complete entire technical process.
2. preparation method according to claim 1, which is characterized in that in step (1), the Reishi sporule powder raw material is adopted
It closes on Korea S's ganoderma lucidum body of artificial cultivation.
3. a kind of the antitumor soft broken wall side of lucidum spore powder enzyme fermentation of anti-aging anticancer is had both according to claim 1
Method, which is characterized in that in step (4), the method for the vacuum freezedrying processing are as follows: dispense the suspension in step (3)
In lyophilized plate, 1-60Pa is vacuumized, filling nitrogen to atmospheric pressure is cooled to 0 DEG C--35 DEG C in advance, after being kept for 0.5-4 hours, takes out
Vacuum 1-60Pa, heating sublimation is to 0-10 DEG C, then is cooled to 0-- 50 DEG C, and suspension is made to be frozen into ice cube solid, keeps 1-15
Hour；1-60Pa is vacuumized again, and heating is biochemical, and 15-30 as a child led to nitrogen to 10-80Pa, rises to 40 DEG C of -6- to temperature of charge
DEG C, 2-16 hours are kept the temperature, discharging.
4. a kind of the antitumor soft broken wall side of lucidum spore powder enzyme fermentation of anti-aging anticancer is had both according to claim 1
Method, which is characterized in that qualification is extracted and preserved using liquid bacterial strain cultivation in ganoderma lucidum parental strain described in step (5)
Parental strain.
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| CN201810825216.XA CN108938681A (en) | 2018-07-25 | 2018-07-25 | It is a kind of to have both the antitumor soft wall-breaking method of lucidum spore powder enzyme fermentation of anti-aging anticancer |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112121965A (en) * | 2020-09-07 | 2020-12-25 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
| CN114948870A (en) * | 2022-06-06 | 2022-08-30 | 安徽金寨仙芝灵生物科技有限公司 | Method for increasing suspension time of wall-broken ganoderma lucidum spore powder |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101596220A (en) * | 2009-04-07 | 2009-12-09 | 西藏月王生物技术有限公司 | A kind of wall-breaking method of Ganoderma spore |
| CN101703531A (en) * | 2009-11-13 | 2010-05-12 | 广州宝兴生物科技有限公司 | Method for refrigerating, vacuum-drying and purifying ganoderma spore powder |
| CN103340909A (en) * | 2013-06-07 | 2013-10-09 | 浙江五养堂药业有限公司 | Biological wall-breaking method of powder |
| CN104017729A (en) * | 2014-06-10 | 2014-09-03 | 信芝（厦门）生物科技有限公司 | Original ecological biological wall-breaking technology of ganoderma lucidum spore powder |
| CN107779400A (en) * | 2016-08-25 | 2018-03-09 | 丹阳西联生物技术有限公司 | A kind of microorganism wall-breaking method of lucidum spore powder |
-
2018
- 2018-07-25 CN CN201810825216.XA patent/CN108938681A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101596220A (en) * | 2009-04-07 | 2009-12-09 | 西藏月王生物技术有限公司 | A kind of wall-breaking method of Ganoderma spore |
| CN101703531A (en) * | 2009-11-13 | 2010-05-12 | 广州宝兴生物科技有限公司 | Method for refrigerating, vacuum-drying and purifying ganoderma spore powder |
| CN103340909A (en) * | 2013-06-07 | 2013-10-09 | 浙江五养堂药业有限公司 | Biological wall-breaking method of powder |
| CN104017729A (en) * | 2014-06-10 | 2014-09-03 | 信芝（厦门）生物科技有限公司 | Original ecological biological wall-breaking technology of ganoderma lucidum spore powder |
| CN107779400A (en) * | 2016-08-25 | 2018-03-09 | 丹阳西联生物技术有限公司 | A kind of microorganism wall-breaking method of lucidum spore powder |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112121965A (en) * | 2020-09-07 | 2020-12-25 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
| CN112121965B (en) * | 2020-09-07 | 2021-12-10 | 宁燕平 | Laser light energy-based spore physical wall breaking device and method |
| CN114948870A (en) * | 2022-06-06 | 2022-08-30 | 安徽金寨仙芝灵生物科技有限公司 | Method for increasing suspension time of wall-broken ganoderma lucidum spore powder |
| CN114948870B (en) * | 2022-06-06 | 2023-10-13 | 安徽金寨仙芝灵生物科技有限公司 | Method for increasing suspension time of wall-broken ganoderma lucidum spore powder |
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