CN106072267A - A kind of highly dissoluble probiotics fermention pollen and preparation and application thereof - Google Patents
A kind of highly dissoluble probiotics fermention pollen and preparation and application thereof Download PDFInfo
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- 235000018291 probiotics Nutrition 0.000 title claims abstract description 48
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 238000000855 fermentation Methods 0.000 claims abstract description 48
- 230000004151 fermentation Effects 0.000 claims abstract description 48
- 108010059892 Cellulase Proteins 0.000 claims abstract description 29
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- 238000000034 method Methods 0.000 claims abstract description 21
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- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 15
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- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical class [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of highly dissoluble probiotics fermention pollen and preparation and application thereof, cellulase is utilized to carry out enzymolysis processing to pollen before fermentation, improve pollen dissolubility and palatability, the glucide that cellulase hydrolysis produces simultaneously again can be as the substrate of lactobacillus-fermented；After fermentation, number of live bacteria of probiotics is up to 109More than CFU/g, and ferment after pollen favorable solubility, brew be difficult to layering, delicate mouthfeel.The technique improves existing probiotics fermention pollen pini dissolubility difference, brew rear easily layering, the shortcoming of mouthfeel unpolished rice, the pollen dissolving mass percent after process, up to more than 95%, significantly improves dissolubility and the palatability of pollen.
Description
(1) technical field
The present invention relates to the preparation of a kind of biofermentation pollen, relate to the preparation of a kind of highly dissoluble probiotics fermention pollen
Technique and tunning thereof and application.
(2) background technology
Pollen pini is a kind of natural high-grade nutrient food, but does not has treated pollen often to have some miscellaneous bacterias, directly
Connecing to eat to make people cause a disease.In addition, pollen pini is with a kind of taste making us and not liking.Meeting nutrition as food
While, it is necessary to there is the good condition of color tool, be just happy to be accepted.Pollen pini can make the crowd of some sensitive physique
Producing allergic reaction, pollen grain has one layer of thicker outer wall, and this layer of outer wall is all more stable to chemical factors such as soda acids, is unfavorable for
Absorbing of nutriment.Probio have in human body intestinal canal anti-mutagenesis, anticancer, improve lactose metabolism rate, reduce serum
Cholesterol, the function of stimulating immune system.In addition, the bacteriocin producing can suppress to produce poison Escherichia coli in food and human body
Growth, thus reduce or reduce some food poisoning risk, also have suppression Giardiasis (Giardia) and pylorus spiral shell in addition
The effect of swing arm bacterium (Helicobacter pylori).
Probio have in human body intestinal canal anti-mutagenesis, anticancer, improve lactose metabolism rate, reduce serum cholesterol, stimulation
Immune function.Additionally, the organic acid producing, bacteriocin can suppress to produce poison life in food and human body for the Escherichia coli
Long breeding, thus reduce or reduce the risk of some things poisoning, also there is suppression giardia lamblia (Giardia lamblia in addition
And the effect of helicobacter pylori (Helicobacter pylori) Stile).Lactic acid bacteria is an important class probio, utilizes it
Pollen is fermented, both can suppress harmful bacteria, can desensitize again, while retaining its nutritional labeling, also can improve its local flavor
And trophic structure.Pollen pini after fermentation has strong lactic acid local flavor, natural pollen pini mouthfeel can be overcome single and weak flavor
Defect, and rich in biodiasmin, and product preferably saves the heat-sensitive substances such as various organized enzyme and vitamin.
Make pollen pini become the nutraceutical of a kind of high protein and low fat by fermentation, have the high-quality battalion of probio health care simultaneously concurrently
Support hardening agent.
Although pollen is the full nutritious and healthy food of a kind of high-quality, but its content of cellulose higher (as pollen pini up to
More than 40%) so that pollen dissolubility is very poor, is easily layered after brewing, mouthfeel is not good.And the pollen fermentation being currently known, all
It is to use merely lactic acid bacteria, saccharomycete etc. to process, although pollen better flavor after fermentation, but be because these probios not
Possess the degradation capability of cellulose, so the pollen dissolubility after Fa Jiao does not have improvement, brew easily layering, mouthfeel unpolished rice.Existing
Having in technology or being the enzymolysis broken wall carrying out pollen individually with cellulase, this only achieves pollen broken wall and improves molten
The effect of solution property, but do not improve the local flavor of pollen；It is individually with probio, pollen to be fermented, improve pollen
Local flavor, the combination achieving probio and pollen, but dissolubility and the palatability of pollen can not be improved.
(3) content of the invention
It is an object of the present invention to provide a kind of highly dissoluble probiotics fermention pollen and preparation and application thereof, it is intended to by sending out
With cellulase, pollen is pre-processed before ferment, two kinds of technology of cellulase hydrolysis broken wall and probiotics fermention are combined,
Improve pollen local flavor, introduce on the basis of probiotic effect, solve existing probiotics fermention pollen dissolubility difference, brew easily layering,
The problems such as mouthfeel unpolished rice, the pollen after process dissolves mass percent up to more than 95%.
The technical solution used in the present invention is:
The present invention provides a kind of highly dissoluble probiotics fermention pollen, and described fermented pollen is prepared as follows: (1) will
It is carried out after pollen collection and sterilization pretreatment, it is thus achieved that pretreated pollen；(2) again by pretreated pollen with aseptic
Water adjustment mass water content, to 60%～80%, adds cellulase, carries out enzyme digestion reaction, obtain under 35～50 DEG C of stirring conditions
Pollen mixture after must digesting；Described cellulase addition with water content 60%～80% pollen quality be calculated as 0.1%～
1%；(3) the pollen mixture after enzymolysis adds precipitated calcium carbonate, mix, inoculate probio, at 30～50 DEG C after mixing
Sealing and fermenting under the conditions of (preferably 35～40 DEG C), 100～200r/min, after fermentation ends, takes tunning, it is thus achieved that after fermentation
Pollen mixture；Described precipitated calcium carbonate quality consumption is calculated as 5%～20% with pollen mixture quality after digesting；Described prebiotic
Mycoplasma amount consumption is calculated as 5%～20% with pollen mixture quality after digesting；(4) while hot to fermentation after pollen mixture in add
With trehalose, mixing and dissolving, freeze-drying to mass water content, below 5%, is pulverized, it is thus achieved that described highly dissoluble probiotics fermention
Pollen；Described trehalose is calculated as 5%～20% with pollen mixture quality after fermenting.
Further, the described preprocess method of step (1) is: timely airing, removal of impurities of sieving, drying after pollen collection, then
Use60Co γ radiation sterilization (dosage 9kGy) carries out sterilization treatment to pollen.
Further, the described pollen of step (1) is edible pollen, including pollen pini, peony pollen, lotus pollen, rape
Flower pollen and zasiokaurin.
Further, the described cellulase addition of step (2) with water content 60%～80% pollen quality be calculated as 0.2%～
0.6%, described cellulase activity is 50U/mg.
Further, step (3) described precipitated calcium carbonate quality consumption with pollen mixture quality after digesting be calculated as 5%～
10%；Described probio quality consumption is calculated as 5%～15% with pollen mixture quality after digesting.
Further, the described trehalose of step (4) is calculated as 5%～15% with pollen mixture quality after fermenting.
Further, the described probio of step (3) is for including Lactobacillus plantarum Lp-115, Lactobacillus casei Lc-11, secondary cheese
Lactobacillus Lpc-37, lactobacillus acidophilus La-14 and lactobacillus bulgaricus Lb-64, be purchased from Du Pont China Holding Co., Ltd.
The bacterium powder of (Shanghai branch company), bacterium powder viable count is 2 × 1010CFU/g。
Further, the described freeze-drying condition of step (4) is vacuum 5～15Pa, cold under the conditions of temperature-55～-63 DEG C
It lyophilized dry is crushed to 200 mesh to mass water content below 5%.
Further, described highly dissoluble probiotics fermention pollen is prepared as follows: (1) dries in the air in time by after pollen collection
Solarization, removal of impurities of sieving, drying, then use60Co γ radiation sterilization (dosage 9kGy) carries out sterilization pretreatment to pollen, it is thus achieved that pre-
Pollen after process；(2) again by pretreated pollen sterilized water adjust mass water content to 60%～80%, add fiber
Element enzyme, carries out enzyme digestion reaction 0.5～5h, it is thus achieved that the pollen mixture after enzymolysis under 35～50 DEG C of stirring conditions；Described fiber
Element enzyme addition is calculated as 0.2%～0.6% with water content 60%～80% pollen quality；(3) the pollen mixture after enzymolysis
Middle addition precipitated calcium carbonate, mixes, and inoculates probio, 35～45 DEG C, sealing and fermenting under the conditions of 100～200r/min after mixing
10～20h, after fermentation ends, takes tunning, it is thus achieved that the pollen mixture after fermentation；Described precipitated calcium carbonate quality consumption with
After enzymolysis, pollen quality is calculated as 5%～10%；Described probio quality consumption with pollen mixture quality after digesting be calculated as 5%～
15%；(4) the pollen mixture after fermentation adds trehalose, mix and dissolve, at vacuum 5～15Pa, temperature-55～-
Freeze-drying under the conditions of 63 DEG C is crushed to 200 mesh to mass water content below 5%, it is thus achieved that described highly dissoluble probiotics fermention
Pollen；Described trehalose is calculated as 5%～15% with the pollen mixture quality after fermenting.
The present invention also provides a kind of described highly dissoluble probiotics fermention pollen answering in preparing food or health food
With described food or health food are with mass ratio 1:0.01～0.1 by highly dissoluble probiotics fermention pollen and antierythrite
Mix (preferably 1:0.05～0.1, more preferably 1:0.1)；Described antierythrite is crushed to 200 mesh.
Probio used by the present invention is that Du Pont China Holding Co., Ltd. (Shanghai branch company) produces and commercialization pin
(bacterium powder viable count is 2 × 10 to the bacterium powder sold10CFU/g), have reliable security, higher lactic acid produces ability and enteron aisle is fixed
Plant ability；Pollen used is the pollen species that state's laws regulation is allowed for food.In sweat, probio utilizes pollen
Contained glucide, metabolism produces the local flavor that the flavor substance such as lactic acid and other organic acids had both improved pollen, inhibits again
Harmful microbe is bred, and technique and tunning are safe and reliable.
Compared with prior art, the present invention has the advantages that: the present invention provides a kind of highly dissoluble probio
Fermented pollen and preparation thereof and application.Utilize cellulase to carry out enzymolysis processing to pollen before fermentation, improve pollen dissolubility
And palatability, the glucide that cellulase hydrolysis produces simultaneously again can be as the substrate of probiotics fermention；After fermentation, probio lives
Bacterium number is up to 109More than CFU/g, and ferment after pollen favorable solubility, brew be difficult to layering, delicate mouthfeel.This technology
Improving existing probiotics fermention pollen pini dissolubility difference, brewing rear easily layering, the shortcoming of mouthfeel unpolished rice, the pollen after process is molten
Solution mass percent, up to more than 95%, significantly improves dissolubility and the palatability of pollen.
(4) detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This:
The present invention provides the preparation technology of a kind of highly dissoluble probiotics fermention pollen, utilizes cellulase to flower before fermentation
Powder carries out enzymolysis processing, improves pollen dissolubility and palatability.Probio used by the embodiment of the present invention is purchased from China of Du Pont
Group Co., Ltd (Shanghai branch company), bacterium powder viable count is 2 × 1010CFU/g, has reliable security, higher lactic acid
Generation ability and intestinal colonisation ability.After fermentation in pollen number of live bacteria of probiotics up to 109More than CFU/g, and after fermenting
Pollen dissolve mass percent up to more than 95%, favorable solubility, brew be difficult to layering, delicate mouthfeel, there is strong breast
Acid local flavor.
Embodiment 1
1) timely airing, removal of impurities of sieving, drying and processing after pollen pini 100g gathers；
2) use60Co γ radiation sterilization (dosage 9kGy) carries out sterilization treatment to pollen pini；
3) adjust pollen pini mass water content to 60% with sterilized water, adjust later pollen quality 250g of water content,
0.5g cellulase (enzyme 50U/mg alive) is added to carry out enzyme digestion reaction in the ratio of mass concentration 0.2%；
4) hydrolysis temperature is 35 DEG C, enzymolysis time 5h, and enzymolysis process is stirred continuously, it is thus achieved that the pollen mixture after enzymolysis
250.5g；
5) in the pollen after enzymolysis processing, add 12.5g precipitated calcium carbonate, mix；
6) access 12.5g Lactobacillus plantarum Lp-115 again to mix；
7) sealing and fermenting, temperature 35 DEG C, speed of agitator 150r/min, fermentation time 14h, take tunning, it is thus achieved that fermentation
After pollen mixture 275.5g；
8), after fermentation ends, add 13.8g trehalose while hot and put tank as freeze drying protectant, mixing after dissolving；
9) it is lyophilized tunning thickness 2cm in pallet, quick freezing repository freezes rapidly；
10) in vacuum 10Pa, freeze-drying under the conditions of temperature-55 DEG C, make moisture be down to 5%；
11) with micronizer, the pollen after freeze-drying is crushed to 200 mesh, it is thus achieved that highly dissoluble probiotics fermention flower
Powder 119.3g；
12) dissolubility test: testing sample used when testing every time is identical in quality, and distilled water volume is also identical.Test
When, testing sample is quickly poured in the distilled water of room temperature (25 DEG C), is gently agitated for 2min with glass bar so that it is fully dissolve；
Then solution 2000r/min centrifuges 5min；The supernatant obtaining is carried out freeze-drying (condition with above), make moisture be down to
Testing sample is consistent.The solid masses now obtaining is the sample quality of dissolving, is the matter of dissolving with the ratio of testing sample
Amount percentage.
13) antierythrite is crushed to 200 mesh；
14) in step 11) highly dissoluble probiotics fermention pollen 119.3g adds step 12) in antierythrite 6g,
Carry out mouthfeel adjustment.
Be not added with cellulase for comparison, other operations are identical；After experiment terminates, measure and relatively more each dissolving quality organized
Percentage, judges dissolubility height with this.After fermentation in pollen number of live bacteria of probiotics up to 3.42 × 109CFU/g, dissolves quality
Percentage is up to 95.9%, and it is 53.1% that control group dissolves mass percent.Pollen favorable solubility after fermentation, brew and be difficult to
Layering, delicate mouthfeel, have strong lactic acid local flavor.
Embodiment 2
1) timely airing, removal of impurities of sieving, drying and processing after zasiokaurin 100g gathers；
2) use60Co γ radiation sterilization (dosage 9kGy) carries out sterilization treatment to zasiokaurin；
3) adjust zasiokaurin mass water content to 70% with sterilized water, adjust the later pollen quality of water content
333.3g, adds 1.3g cellulase (enzyme 50U/mg alive) to carry out enzyme digestion reaction in the ratio of mass concentration 0.4%；
4) hydrolysis temperature is 40 DEG C, enzymolysis time 1h, and enzymolysis process is stirred continuously, it is thus achieved that the pollen mixture after enzymolysis
334.6g；
5) in the pollen mixture after enzymolysis processing, add 23.4g precipitated calcium carbonate, mix；
6) access 33.5g Lactobacillus casei Lc-11 again to mix；
7) sealing and fermenting, temperature 35 DEG C, speed of agitator 100r/min, fermentation time 16h, it is thus achieved that tunning 391.5g；
8), after fermentation ends, add 39.2g trehalose while hot and put tank as freeze drying protectant, mixing after dissolving；
9) it is lyophilized tunning thickness 1cm in pallet, quick freezing repository freezes rapidly；
10) in vacuum 10Pa, freeze-drying under the conditions of temperature-60 DEG C, make moisture be down to 5%；
11) with micronizer, the pollen after freeze-drying is crushed to 200 mesh, it is thus achieved that highly dissoluble probiotics fermention flower
Powder 167.4g；
12) antierythrite is crushed to 200 mesh；
13) in step 11) highly dissoluble probiotics fermention pollen 167.4g adds step 12) in antierythrite
16.74g, carries out mouthfeel adjustment.
Be not added with cellulase for comparison, other operations are identical；After experiment terminates, measure and relatively more each dissolving quality organized
Percentage, judges dissolubility height with this.Dissolubility is tested: method is same as in Example 1.Probiotics viable bacteria in pollen after fermentation
Number is up to 8.67 × 109CFU/g, dissolving mass percent is up to 96.2%, and it is 65.4 that control group dissolves mass percent.Fermentation
After pollen favorable solubility, brew be difficult to layering, delicate mouthfeel, there is strong lactic acid local flavor.
Embodiment 3
1) timely airing, removal of impurities of sieving, drying and processing after pollen pini 100g gathers；
2) use60Co γ radiation sterilization (dosage 9kGy) carries out sterilization treatment to pollen pini；
3) adjust pollen pini mass water content to 80% with sterilized water, adjust later pollen quality 500g of water content,
3g cellulase (enzyme 50U/mg alive) is added to carry out enzyme digestion reaction in the ratio of mass concentration 0.6%；
4) hydrolysis temperature is 50 DEG C, enzymolysis time 0.5h, and enzymolysis process is stirred continuously, it is thus achieved that the pollen mixture after enzymolysis
500.3g；
5) in the pollen mixture after enzymolysis processing, add 50g precipitated calcium carbonate, mix；
6) access 75g Lactobacillus paracasei Lpc-37 in the ratio of mass concentration inoculum concentration 15% again to mix；
7) sealing and fermenting, temperature 40 DEG C, speed of agitator 200r/min, fermentation time 20h, it is thus achieved that tunning 625.3g；
8), after fermentation ends, add 93.8g trehalose while hot and put tank as freeze drying protectant, mixing after dissolving；
9) it is lyophilized tunning thickness 2cm in pallet, quick freezing repository freezes rapidly；
10) in vacuum 15Pa, freeze-drying under the conditions of temperature-55 DEG C, make moisture be down to 5%；
11) with micronizer, the pollen after freeze-drying is crushed to 200 mesh, it is thus achieved that highly dissoluble probiotics fermention flower
Powder 301.8g；
12) antierythrite is crushed to 200 mesh；
13) in step 11) highly dissoluble probiotics fermention pollen 301.8g adds step 12) in antierythrite
41.3g, carries out mouthfeel adjustment.
Be not added with cellulase for comparison, other operations are identical；After experiment terminates, measure and relatively more each dissolving quality organized
Percentage, judges dissolubility height with this.Dissolubility is tested: method is same as in Example 1.Probiotics viable bacteria in pollen after fermentation
Number is up to 5.31 × 109CFU/g, dissolving mass percent is up to 95.7%, and it is 57.8% that control group dissolves mass percent.Send out
Pollen favorable solubility after ferment, brew and be difficult to layering, delicate mouthfeel, there is strong lactic acid local flavor.
Embodiment 4
1) timely airing, removal of impurities of sieving, drying and processing after peony pollen 100g gathers；
2) use60Co γ radiation sterilization (dosage 9kGy) carries out sterilization treatment to peony pollen；
3) adjust peony pollen mass water content to 70% with sterilized water, adjust the later pollen quality of water content
333.3g, adds 1.7g cellulase (enzyme 50U/mg alive) to carry out enzyme digestion reaction in the ratio of mass concentration 0.5%；
4) hydrolysis temperature is 40 DEG C, enzymolysis time 1h, and enzymolysis process is stirred continuously, it is thus achieved that the pollen mixture after enzymolysis
335g；
5) in the peony pollen mixture after enzymolysis processing, add 23.5g precipitated calcium carbonate, mix；
6) in terms of the pollen mixture gross mass after enzymolysis, 33.5g Bao Jiali is accessed in the ratio of quality inoculum concentration 10%
Sub-lactobacillus Lb-64 mixes；
7) sealing and fermenting, temperature 35 DEG C, speed of agitator 100r/min, fermentation time 16h, it is thus achieved that tunning 392g；
8), after fermentation ends, add 39.2g trehalose while hot and put tank as freeze drying protectant, mixing after dissolving；
9) it is lyophilized tunning thickness 1cm in pallet, quick freezing repository freezes rapidly；
10) in vacuum 10Pa, freeze-drying under the conditions of temperature-60 DEG C, make moisture be down to 5%；
11) with micronizer, the peony pollen after freeze-drying is crushed to 200 mesh, it is thus achieved that probio sends out highly dissoluble
Ferment pollen 167.9g；
12) antierythrite is crushed to 200 mesh；
13) in step 11) highly dissoluble probiotics fermention pollen 167.9g adds step 12) in antierythrite
16.8g, carries out mouthfeel adjustment.
Be not added with cellulase for comparison, other operations are identical；After experiment terminates, measure and relatively more each dissolving quality organized
Percentage, judges dissolubility height with this.Dissolubility is tested: method is same as in Example 1.Probio in peony pollen after fermentation
Viable count is up to 8.67 × 109CFU/g, dissolving mass percent is up to 95.8%, and control group dissolves mass percent and is
63.5%.Peony pollen favorable solubility after fermentation, brew and be difficult to layering, delicate mouthfeel, there is strong lactic acid local flavor.
Embodiment 5
1) timely airing, removal of impurities of sieving, drying and processing after lotus pollen 100g gathers；
2) use60Co γ radiation sterilization (dosage 9kGy) carries out sterilization treatment to lotus pollen；
3) adjust lotus pollen mass water content to 70% with sterilized water, adjust the later pollen quality of water content
333.3g, adds 1.7g cellulase (enzyme 50U/mg alive) to carry out enzyme digestion reaction in the ratio of mass concentration 0.5%；
4) hydrolysis temperature is 40 DEG C, enzymolysis time 1h, and enzymolysis process is stirred continuously, it is thus achieved that the pollen mixture after enzymolysis
335g；
5) in the lotus pollen mixture after enzymolysis processing, add 23.5g precipitated calcium carbonate, mix；
6) in terms of the pollen mixture gross mass after enzymolysis, 33.5g is accessed addicted to yogurt in the ratio of quality inoculum concentration 10%
Bacillus La-14 mixes；
7) sealing and fermenting, temperature 35 DEG C, speed of agitator 100r/min, fermentation time 16h, it is thus achieved that tunning 392g；
8), after fermentation ends, add 39.2g trehalose while hot and put tank as freeze drying protectant, mixing after dissolving；
9) it is lyophilized tunning thickness 1cm in pallet, quick freezing repository freezes rapidly；
10) in vacuum 10Pa, freeze-drying under the conditions of temperature-60 DEG C, make moisture be down to 5%；
11) with micronizer, the lotus pollen after freeze-drying is crushed to 200 mesh, it is thus achieved that probio sends out highly dissoluble
Ferment pollen 167.3g；
12) antierythrite is crushed to 200 mesh；
13) in step 11) highly dissoluble probiotics fermention pollen 167.3g adds step 12) in antierythrite
16.8g, carries out mouthfeel adjustment.
Be not added with cellulase for comparison, other operations are identical；After experiment terminates, measure and relatively more each dissolving quality organized
Percentage, judges dissolubility height with this.Dissolubility is tested: method is same as in Example 1.Probio in lotus pollen after fermentation
Viable count is up to 8.67 × 109CFU/g, dissolving mass percent is up to 96.4%, and control group dissolves mass percent and is
62.1%.Lotus pollen favorable solubility after fermentation, brew and be difficult to layering, delicate mouthfeel, there is strong lactic acid local flavor.
Comparative example
For further illustrating the superiority of highly dissoluble probiotics fermention pollen preparation technology of the present invention, below in conjunction with
Pollen individually carries out digesting or individually carries out probiotics fermention, the superiority of the comparative illustration present invention.
Individually carrying out the pollen of enzymolysis processing, for comparing the present invention, its dissolubility is significant lower, dissolves percent mass
Number is averagely lower by about 10% than the present invention, and product does not exist probiotics viable bacteria, and product local flavor does not improve；And individually carry out benefit
The pollen viable count of raw bacterium fermentation low two orders of magnitude than the present invention, only 107CFU/g, dissolves mass percent and process
Front fair, namely its dissolubility does not improve.
Comparative example 1
1) timely airing, removal of impurities of sieving, drying and processing after pollen pini 100g gathers；
2) use60Co γ radiation sterilization (dosage 9kGy) carries out sterilization treatment to pollen pini；
3) adjust pollen pini mass water content to 60% with sterilized water, adjust later pollen quality 250g of water content；
4) to adjust pollen compounding substances gauge after water content, add 12.5g precipitated calcium carbonate, mix；
6) to adjust pollen compounding substances gauge after water content, mix by accessing 12.5g Lactobacillus plantarum Lp-115；
7) sealing and fermenting, temperature 35 DEG C, speed of agitator 150r/min, fermentation time 14h, take tunning, it is thus achieved that fermentation
After pollen mixture 275g；
8) after fermentation ends, with the pollen compounding substances gauge after fermentation, 13.8g trehalose is added while hot as lyophilized guarantor
Protect agent, mix and put tank after dissolving；
9) it is lyophilized tunning thickness 2cm in pallet, quick freezing repository freezes rapidly；
10) in vacuum 10Pa, freeze-drying under the conditions of temperature-55 DEG C, make moisture be down to 5%；
11) with micronizer, the pollen after freeze-drying is crushed to 200 mesh, it is thus achieved that probiotics fermention pollen
118.8g；
12) antierythrite is crushed to 200 mesh；
13) in step 11) probiotics fermention pollen 118.8g adds step 12) in antierythrite 6g, carry out mouthfeel
Adjust.
Control group is with the maltodextrin substituted plant lactobacillus Lp-115 bacterium powder of equal in quality, and other operations are identical；Experiment
Detecting after end, dissolubility method of testing is same as in Example 1.In experimental group pollen number of live bacteria of probiotics up to 2.32 ×
107CFU/g, control group is 0；It is 54.7% that experimental group dissolves mass percent, and control group is 54.7%.Pollen tool after fermentation
Have a strong lactic acid local flavor, but dissolubility is not good enough, brew after layering, mouthfeel have the sensation of unpolished rice.
Comparative example 2
1) timely airing, removal of impurities of sieving, drying and processing after pollen pini 100g gathers；
2) use60Co γ radiation sterilization (dosage 9kGy) carries out sterilization treatment to pollen pini；
3) adjust pollen pini mass water content to 60% with sterilized water, adjust later pollen quality 250g of water content,
0.5g cellulase (enzyme 50U/mg alive) is added to carry out enzyme digestion reaction in the ratio of mass concentration 0.2%；
4) hydrolysis temperature is 35 DEG C, enzymolysis time 5h, and enzymolysis process is stirred continuously, it is thus achieved that the pollen mixture after enzymolysis
250.5g；
5) it is lyophilized the pollen mixture thickness 2cm after enzymolysis in pallet, quick freezing repository freezes rapidly；
6) in vacuum 10Pa, freeze-drying under the conditions of temperature-55 DEG C, make moisture be down to 5%；
7) with micronizer, the pollen after freeze-drying is crushed to 200 mesh, it is thus achieved that the pollen 97g after enzymolysis broken wall；
8) antierythrite is crushed to 200 mesh；
9) in step 7) enzymolysis broken wall after pollen 97g in add step 8) in antierythrite 5.8g, carry out mouthfeel tune
Whole.
Control group is with the maltodextrin alternative fiber element enzyme of equal in quality, and other operations are identical；Experiment is examined after terminating
Surveying, dissolubility method of testing is same as in Example 1.It is 86.1% that experimental group dissolves mass percent, and control group is 61%；Experiment
Group is all 0 with the number of live bacteria of probiotics of control group.Pollen dissolubility after enzymolysis preferably, brew and be difficult to layering, delicate mouthfeel, but
Pollen local flavor does not improve.
Claims (10)
1. a highly dissoluble probiotics fermention pollen, it is characterised in that described fermented pollen is prepared as follows: (1) will flower
It is carried out and sterilization pretreatment after powder collection, it is thus achieved that pretreated pollen；(2) again by pretreated pollen sterilized water
Adjustment mass water content, to 60%～80%, adds cellulase, carries out enzyme digestion reaction under 35～50 DEG C of stirring conditions, it is thus achieved that
Pollen mixture after enzymolysis；Described cellulase addition with water content 60%～80% pollen quality be calculated as 0.1%～
1%；(3) to enzymolysis after pollen mixture in add precipitated calcium carbonate, mix, inoculate probio, after mixing 30～50 DEG C,
Sealing and fermenting under the conditions of 100～200r/min, after fermentation ends, takes tunning, it is thus achieved that the pollen mixture after fermentation；Described
Precipitated calcium carbonate quality consumption is calculated as 5%～20% with the pollen mixture quality after digesting；Described probio quality consumption with
Pollen mixture quality after enzymolysis is calculated as 5%～20%；(4) the pollen mixture after fermentation adds trehalose, mix
Dissolving, freeze-drying to mass water content, below 5%, is pulverized, it is thus achieved that described highly dissoluble probiotics fermention pollen；Described marine alga
Sugar is calculated as 5%～20% with the pollen mixture quality after fermenting.
2. highly dissoluble probiotics fermention pollen as claimed in claim 1, it is characterised in that the described preprocess method of step (1)
For: timely airing, removal of impurities of sieving, drying after pollen collection, then use60Pollen is carried out at sterilizing by Co γ radiation sterilization
Reason.
3. highly dissoluble probiotics fermention pollen as claimed in claim 1, it is characterised in that the described pollen of step (1) includes preserved egg
Powder, peony pollen, lotus pollen, rape petal pollen and zasiokaurin.
4. highly dissoluble probiotics fermention pollen as claimed in claim 1, it is characterised in that the described cellulase of step (2) adds
Amount is calculated as 0.2%～0.6% with water content 60%～80% pollen quality, and described cellulase activity is 50U/mg.
5. highly dissoluble probiotics fermention pollen as claimed in claim 1, it is characterised in that described precipitated calcium carbonate quality consumption
It is calculated as 5%～10% with pollen mixture quality after enzymolysis；Described probio quality consumption is with pollen mixture quality after digesting
It is calculated as 5%～15%；Described trehalose is calculated as 5%～15% with pollen mixture quality after fermenting.
6. highly dissoluble probiotics fermention pollen as claimed in claim 1, it is characterised in that described probio includes planting step (3)
Thing lactobacillus, Lactobacillus casei, Lactobacillus paracasei, lactobacillus acidophilus and lactobacillus bulgaricus.
7. highly dissoluble probiotics fermention pollen as claimed in claim 1, it is characterised in that the described freeze-drying condition of step (4)
For vacuum 5～15Pa, freeze-drying under the conditions of temperature-55～-63 DEG C is crushed to 200 mesh to mass water content below 5%.
8. highly dissoluble probiotics fermention pollen as claimed in claim 1, it is characterised in that described fermented pollen is as follows
Preparation: (1), by airing timely after pollen collection, removal of impurities of sieving, drying, then uses60Pollen is carried out by Co γ radiation sterilization
Sterilization pretreatment, it is thus achieved that pretreated pollen；(2) more pretreated pollen sterilized water is adjusted mass water content extremely
60%～80%, add cellulase, under 35～50 DEG C of stirring conditions, carry out enzyme digestion reaction 0.5～5h, it is thus achieved that after enzymolysis
Pollen mixture；Described cellulase addition is calculated as 0.2%～0.6% with water content 60%～80% pollen quality；(3) to
In pollen mixture after enzymolysis add precipitated calcium carbonate, mix, inoculate probio, after mixing 35～45 DEG C, 100～
Sealing and fermenting 10～20h under the conditions of 200r/min, after fermentation ends, it is thus achieved that the pollen mixture after fermentation；Described lightweight carbonic acid
Calcareous amount consumption is calculated as 5%～10% with pollen mixture quality after digesting；Described probio quality consumption is with pollen after digesting
Mixture quality is calculated as 5%～15%；(4) the pollen mixture after fermentation adds trehalose, mixes and dissolves, in vacuum 5
～15Pa, freeze-drying under the conditions of temperature-55～-63 DEG C is crushed to 200 mesh to mass water content below 5%, it is thus achieved that described height
Dissolubility probiotics fermention pollen；Described trehalose is calculated as 5%～15% with pollen mixture quality after fermenting.
9. application in preparing food or health food for the highly dissoluble probiotics fermention pollen described in a claim 1.
10. apply as claimed in claim 9, it is characterised in that described food or health food are to be sent out by highly dissoluble probio
Ferment pollen mixes with mass ratio 1:0.01～0.1 with antierythrite.
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| CN107136298A (en) * | 2017-05-04 | 2017-09-08 | 凤台硕力畜禽养殖有限公司 | One kind makes an addition to fermented pollen polysaccharide in feed and prepares probiotics |
| CN108029918A (en) * | 2017-12-27 | 2018-05-15 | 浙江亚林生物科技股份有限公司 | One kind is rich in flavone compound solid beverage and preparation method thereof |
| CN109430783A (en) * | 2018-11-12 | 2019-03-08 | 湖南德海制药有限公司 | A kind of enzyme solution of pollen |
| IT201800007229A1 (en) * | 2018-07-16 | 2020-01-16 | MICROBIOLOGICAL PROCESS FOR THE PRODUCTION OF BEE BREAD | |
| CN111671084A (en) * | 2020-06-23 | 2020-09-18 | 烟台新时代健康产业有限公司 | Preparation method of composite enzymolysis-fermentation coupled fermented pine pollen extract |
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| CN107136298A (en) * | 2017-05-04 | 2017-09-08 | 凤台硕力畜禽养殖有限公司 | One kind makes an addition to fermented pollen polysaccharide in feed and prepares probiotics |
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| CN114052113A (en) * | 2021-11-01 | 2022-02-18 | 菏泽尧舜牡丹生物科技有限公司 | Preparation method of pollen tablet candy |
| CN114081854A (en) * | 2021-11-19 | 2022-02-25 | 烟台新时代健康产业日化有限公司 | Pollen pini extract with barrier repair function and fermentation preparation process thereof |
| CN114081854B (en) * | 2021-11-19 | 2023-08-15 | 烟台新时代健康产业日化有限公司 | Pollen pini extract with barrier repairing function and fermentation preparation process thereof |
| CN114209624A (en) * | 2021-12-24 | 2022-03-22 | 美出莱(杭州)化妆品有限责任公司 | Peony fermentation stock solution and preparation method and application thereof |
| CN114209624B (en) * | 2021-12-24 | 2022-09-20 | 美出莱(杭州)化妆品有限责任公司 | Peony fermentation stock solution and preparation method and application thereof |
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