CN109395062B

CN109395062B - Female pudendum sterilization care solution

Info

Publication number: CN109395062B
Application number: CN201811630496.5A
Authority: CN
Inventors: 张明灯; 王琳; 林成燊
Original assignee: Guangzhou Tairan Biotechnology Co ltd
Current assignee: SHAANXI MEIFUKANG BIOLOGICAL TECHNOLOGY CO.,LTD.
Priority date: 2018-12-29
Filing date: 2018-12-29
Publication date: 2021-12-14
Anticipated expiration: 2038-12-29
Also published as: CN109395062A

Abstract

The invention belongs to the field of female daily necessities, and particularly relates to a female pudendum sterilization care solution. The invention provides a female pudendum sterilization care solution which comprises antibacterial polypeptide, a preservative, deionized water and a pH regulator. The care solution can effectively inhibit pathogenic bacteria of female pudendum, and reduce irritation and toxic and side effects of the care solution. Meanwhile, the invention has simple formula and low cost, is suitable for large-scale production and has wide application prospect.

Description

Female pudendum sterilization care solution

Technical Field

The invention belongs to the field of female daily necessities, and particularly relates to a female pudendum sterilization care solution.

Background

The vaginal environment of women is very complex, and is very easy to be infected by pathogenic bacteria and cause related diseases. Research shows that when pathogenic bacteria invade the vaginal environment, the bacteria can rapidly propagate to destroy the acidic microenvironment in the vaginal environment, and symptoms are produced. The female pudendum care solution is a female daily application maintenance solution, and has the main functions of inhibiting pathogenic bacteria, cleaning vaginal environment and adjusting pH.

Most of the commonly used vaginal care solutions at present contain plant extracts or animal extracts, such as chitosan and the like. These antibacterial agents act as "foreign molecules" that can irritate the vagina and disrupt the vaginal microenvironment. The antibacterial polypeptide is an antibacterial molecule consisting of amino acids, has a molecular weight of about 1000-7000 and consists of 20-60 amino acid residues. The antibacterial polypeptide widely exists in human bodies, has the advantages of broad-spectrum antibacterial property, low drug resistance, non-toxic and side-stimulating effect and the like as an antibacterial agent, and is concerned by researchers. However, reports of the antibacterial polypeptide used as an antibacterial component of female pudendum care solution are not seen.

Therefore, the female pudendum sterilization care solution is developed based on the antibacterial polypeptide, so that the pathogenic bacteria of the female pudendum can be effectively inhibited, the irritation and the toxic and side effects of the care solution are reduced, and the application prospect is wide.

Disclosure of Invention

In view of the above, the invention provides a female pudendum sterilization care solution based on antibacterial polypeptide, which can effectively inhibit pathogenic bacteria of female pudendum and reduce irritation and toxic and side effects of the care solution.

The invention provides a female pudendum sterilization care solution, which comprises the following components: antibacterial polypeptide, antiseptic, deionized water, and pH regulator.

Preferably, the antibacterial polypeptide sequence is LRKSKEKIGKEFKRRKDFLRNLV.

Preferably, the preservative is glyceryl monocaprylate.

Preferably, the pH regulator is 10% hydrochloric acid aqueous solution in volume percentage

Preferably, the composition comprises, in mass percent: 1 to 3 percent of antibacterial polypeptide, 0.3 to 0.8 percent of preservative, 94.7 to 98.2 percent of deionized water, 0.5 to 1.5 percent of pH regulator and 2.0 to 5.2 percent of pH.

Preferably, the composition comprises, in mass percent: 2.1 percent of antibacterial polypeptide, 0.5 percent of preservative, 96.6 percent of deionized water, 0.8 percent of pH regulator and 3.4 percent of pH.

The invention also provides application of the composition in the field of female pudendum sterilization.

The invention also aims to provide a preparation method of the female pudendum sterilization care solution, which comprises the following steps:

(1) weighing 1-3% of antibacterial polypeptide, 0.3-0.8% of preservative, 0.5-1.5% of pH regulator and the balance of deionized water according to mass percentage.

(2) And (2) mixing the antibacterial polypeptide, the preservative and part of the pH regulator in the step (1) with deionized water, and uniformly stirring.

(3) Further adjusting the pH of the mixed solution to 2.0-5.2 by using a pH regulator.

(4) Stirring to be clear, and obtaining the female pudendum sterilization care solution.

In summary, the present invention provides a composition comprising: antibacterial polypeptide, antiseptic, deionized water and pH regulator. The antibacterial polypeptide is used as an antibacterial agent, so that the problem of biostimulation of the traditional antibacterial agent is solved while the excellent antibacterial property is maintained, pudendum bacteria can be effectively killed, and the internal environment stability of the pudendum is maintained. Meanwhile, the invention has simple formula and low cost.

Detailed Description

The invention provides a female pudendum sterilization care solution. The technical solutions in the embodiments of the present invention will be described below. The described embodiments are only some, not all embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Specific examples are as follows;

example 1

Weighing 2.1 parts of antibacterial polypeptide, 0.5 part of preservative, 96.6 parts of deionized water and 0.8 part of pH regulator according to mass percentage. And mixing and stirring the components according to the method until the components are clear, and obtaining the female pudendum sterilization care solution. At this time, the pH of the solution was 3.4.

Example 2

Weighing 1 part of antibacterial polypeptide, 0.3 part of preservative, 98.2 parts of deionized water and 0.5 part of pH regulator according to mass percentage. And mixing and stirring the components according to the method until the components are clear, and obtaining the female pudendum sterilization care solution. At this time, the pH of the solution was 5.2.

Example 3

Weighing 3 parts of antibacterial polypeptide, 0.5 part of preservative, 95.3 parts of deionized water and 1.2 parts of pH regulator according to mass percentage. And mixing and stirring the components according to the method until the components are clear, and obtaining the female pudendum sterilization care solution. At this time, the pH of the solution was 2.8.

Example 4

Weighing 1.8 parts of antibacterial polypeptide, 0.8 part of preservative, 95.9 parts of deionized water and 1.5 parts of pH regulator according to mass percentage. And mixing and stirring the components according to the method until the components are clear, and obtaining the female pudendum sterilization care solution. At this time, the pH of the solution was 2.1.

Example 5

Weighing 3 parts of antibacterial polypeptide, 0.8 part of preservative, 94.7 parts of deionized water and 1.5 parts of pH regulator according to mass percentage. And mixing and stirring the components according to the method until the components are clear, and obtaining the female pudendum sterilization care solution. At this time, the pH of the solution was 2.0.

Example 6

This example is a bacteriostatic test of the care solution products prepared in examples 1-5.

(1) In the invention, common pathogenic bacteria of female pudendum, such as escherichia coli, staphylococcus aureus, pseudomonas aeruginosa and bacillus subtilis, are selected as experimental strains. Each strain was activated in broth at a shaker speed of 150rpm at 37 ℃ for 8 hours for use.

(2) The solutions 1-5 were sterilized at 121 ℃ for 20min and added to sterile broth medium at a concentration of 0.05% (w/v). Meanwhile, a broth culture medium without a care solution product is added with deionized water with equal mass and sterilized for 20min at 121 ℃ to replace the care solution as a control group for carrying out experiments.

(3) 1mL of activated bacteria solution (bacteria concentration 10) was taken under aseptic conditions⁹cfu/mL) was added to 49mL of the medium prepared in the above step (2), incubated at 37 ℃ and a shaker rotation speed of 150rpm for 6 hours, and then characterized by plate counting after dilution according to the national standard GB 4789.2-1994.

(4) The sterilization rate was calculated according to the following formula:

the sterilization rate is (blank group bacteria number-experimental group bacteria number)/blank group bacteria number

Wherein the number of bacteria is cfu/mL.

The results of the experiment are shown in table 1.

TABLE 1 Sterilization rates of different Care solutions against four bacteria

	Escherichia coli	Staphylococcus aureus	Pseudomonas aeruginosa	Bacillus subtilis
					Care solution 1	99.92％	99.96％	99.91％	99.89％
Care solution 2	82.10％	83.54％	76.23％	90.09％
					Care solution 3	96.54％	97.89％	97.28％	93.21％
Care solution 4	90.03％	88.92％	87.87％	93.98％
					Care solution 5	98.12％	98.92％	96.79％	99.10％

The result shows that the care solution product has certain bactericidal effect on escherichia coli, staphylococcus aureus, pseudomonas aeruginosa and bacillus subtilis, wherein the care solution prepared in the example 1 has the best bactericidal effect.

In summary, the invention provides a female pudendum care solution and a preparation method thereof, and the care solution comprises the following components: antibacterial polypeptide, antiseptic, deionized water, and pH regulator. The care solution can effectively inhibit the breeding of female pudendum bacteria and reduce the stimulation to skin. Meanwhile, the invention has simple formula and low cost, and is suitable for mass production.

The foregoing is only a preferred embodiment of the invention. Modifications thereof, which would occur to persons skilled in the art without departing from the principles of the invention, are also considered to be within the scope of the invention.

Claims

1. The female pudendum sterilization care solution is characterized by comprising the following components: the antibacterial peptide comprises an antibacterial peptide, a preservative, deionized water and a pH regulator, wherein the antibacterial peptide has a sequence of LRKSKEKIGKEFKRRKDFLRNLV.

2. The female pudendal bactericidal care solution according to claim 1, wherein the preservative is glyceryl monocaprylate.

3. The female pudendal bactericidal care solution of claim 1, wherein said pH regulator is 10% by volume aqueous hydrochloric acid.

4. The female pudendum sterilizing care solution as defined in claim 1, wherein the care solution comprises, by mass: 1 to 3 percent of antibacterial polypeptide, 0.3 to 0.8 percent of preservative, 94.7 to 98.2 percent of deionized water, 0.5 to 1.5 percent of pH regulator and 2.0 to 5.2 percent of pH.

5. The female pudendum sterilizing care solution according to claim 4, wherein the care solution comprises, by mass, 2.1% of the antibacterial polypeptide, 0.5% of the preservative, 96.6% of deionized water, 0.8% of the pH regulator, and the pH is 3.4.

6. The female pudendum sterilizing care solution as claimed in claim 4, wherein the preparation method comprises the following steps:

(1) weighing 1-3% of antibacterial polypeptide, 0.3-0.8% of preservative, 0.5-1.5% of pH regulator and the balance of deionized water according to the mass percentage;

(2) mixing the antibacterial polypeptide, the preservative and part of the pH regulator in the step (1) with deionized water, and uniformly stirring;

(3) further adjusting the pH value of the mixed solution to 2.0-5.2 by using a pH regulator;