CN109512692B

CN109512692B - Antibacterial additive solution for wet tissue and preparation method thereof

Info

Publication number: CN109512692B
Application number: CN201811633665.0A
Authority: CN
Inventors: 王琳; 林成燊
Original assignee: Zhejiang Guoneng Technology Co ltd
Current assignee: ZHEJIANG GUONENG TECHNOLOGY Co.,Ltd.
Priority date: 2018-12-29
Filing date: 2018-12-29
Publication date: 2021-09-07
Anticipated expiration: 2038-12-29
Also published as: CN109512692A

Abstract

The invention discloses an antibacterial additive solution for wet tissues, which consists of the following substances in percentage by mass: 3% -8% of antibacterial polypeptide; 1 to 1.5 percent of humectant, 0.1 to 0.5 percent of pH regulator, 0.4 to 0.6 percent of emollient and the balance of deionized water. The antibacterial polypeptide sequence is LRKSKEKIGKEFKRRKDFLRNLV, the humectant is glycerin, the pH regulator is citric acid, the emollient is polydimethylsiloxane, and the pH value of the additive solution is 3.5-6.8. The additive solution prepared by the invention has excellent antibacterial performance, has a pH value close to that of human skin, is non-irritant, low in price and low in production cost, and has a wide application prospect.

Description

Antibacterial additive solution for wet tissue and preparation method thereof

Technical Field

The invention belongs to the field of daily necessities, and particularly relates to an antibacterial additive solution for wet tissues.

Background

In daily life, bacteria are ubiquitous. Bacteria can adhere to the surface of human skin and enter the body through such means as feeding, wounds, etc., causing infection and disease. With the continuous improvement of the living standard of people, in daily life, the wet tissue, especially the wet tissue with the antibacterial function is often used for wiping the skin, so that the bacterial infection can be effectively inhibited, and related diseases can be prevented.

However, currently, there are still certain problems with antibacterial wet wipes. Most of the antibiotic additive liquid for wet tissues uses artificially synthesized chemical antibiotic agents as antibiotic materials. These chemical antiseptics irritate human skin, especially infant skin and wounded skin, and give people an uncomfortable feeling. Therefore, aiming at the problem, a safer and more reliable antibacterial additive solution for wet tissues is provided, so that the wet tissues have excellent antibacterial performance and do not irritate the skin, and the key technical problem which needs to be solved in the field is urgently provided.

Disclosure of Invention

In view of the above, the invention provides an antibacterial additive solution for wet tissues and a preparation method thereof based on antibacterial polypeptides, which can effectively inhibit bacteria adhered to the surface of skin in daily life and reduce the irritation and toxic and side effects of antibacterial solution.

The invention provides an antibacterial additive solution for wet tissues, which comprises the following components: antibacterial polypeptide, humectant, pH regulator, emollient and deionized water.

Preferably, the antibacterial polypeptide sequence is LRKSKEKIGKEFKRRKDFLRNLV.

Preferably, the humectant is glycerin.

Preferably, the pH adjusting agent is citric acid.

Preferably, the emollient is polydimethylsiloxane.

Preferably, the composition comprises, in mass percent: 3% -8% of antibacterial polypeptide; 1 to 1.5 percent of humectant, 0.1 to 0.5 percent of pH regulator, 0.4 to 0.6 percent of emollient and the balance of deionized water, and the pH value of the additive solution is 3.5 to 6.8.

Preferably, the composition comprises, in mass percent: 6.2 percent of antibacterial polypeptide, 1.2 percent of humectant, 0.3 percent of pH regulator, 0.5 percent of emollient and the balance of deionized water, and the pH value is 4.8.

The invention also provides application of the antibacterial additive solution for the wet tissue in the field of daily sterilization.

The invention also aims to provide a preparation method of the antibacterial additive solution for the wet tissue, which comprises the following steps:

(1) 3% -8% of antibacterial polypeptide is weighed according to mass percentage; 1 to 1.5 percent of humectant, 0.1 to 0.5 percent of pH regulator, 0.4 to 0.6 percent of emollient and the balance of deionized water.

(2) And (2) mixing the antibacterial polypeptide, the humectant, the emollient and part of the pH regulator in the step (1) with deionized water, and uniformly stirring.

(3) Further adjusting the pH of the mixed solution to 3.5-6.8 by using a pH regulator.

(4) Stirring until the mixture is clear, and obtaining the antibacterial additive solution for the wet tissue.

In summary, the invention provides an antibacterial additive solution for wet tissues, which comprises: antibacterial polypeptide, humectant, pH regulator, emollient and deionized water. The antibacterial polypeptide is used as an antibacterial agent, so that the problem of biostimulation of the traditional antibacterial agent is solved while the excellent antibacterial property is maintained, and common pathogenic bacteria in daily life can be effectively killed. Meanwhile, the invention has simple formula and low cost, and is suitable for mass production.

Detailed Description

The invention provides an antibacterial additive solution for wet tissues. The technical solutions in the embodiments of the present invention will be described below. The described embodiments are only some, not all embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Specific examples are as follows;

example 1

Weighing 6.2 parts of antibacterial polypeptide, 1.2 parts of humectant, 0.3 part of pH regulator, 0.5 part of emollient and 91.8 parts of deionized water according to mass percentage. And mixing and stirring the components according to the method until the components are clear, so as to obtain the antibacterial additive solution for the wet tissue. At this time, the pH of the antimicrobial additive solution for wet tissues was 4.8.

Example 2

Weighing 3 parts of antibacterial polypeptide, 1 part of humectant, 0.1 part of pH regulator, 0.4 part of emollient and 95.5 parts of deionized water according to mass percentage. And mixing and stirring the components according to the method until the components are clear, so as to obtain the antibacterial additive solution for the wet tissue. At this time, the pH of the antimicrobial additive solution for wet tissues was 6.8.

Example 3

Weighing 8 parts of antibacterial polypeptide, 1.5 parts of humectant, 0.5 part of pH regulator, 0.6 part of emollient and 89.4 parts of deionized water according to mass percentage. And mixing and stirring the components according to the method until the components are clear, so as to obtain the antibacterial additive solution for the wet tissue. At this time, the pH of the antimicrobial additive solution for wet tissues was 3.5.

Example 4

Weighing 4.5 parts of antibacterial polypeptide, 1.2 parts of humectant, 0.28 part of pH regulator, 0.48 part of emollient and 93.52 parts of deionized water according to mass percentage. And mixing and stirring the components according to the method until the components are clear, so as to obtain the antibacterial additive solution for the wet tissue. At this time, the pH of the antimicrobial additive solution for wet tissues was 4.5.

Example 5

Weighing 7 parts of antibacterial polypeptide, 1.4 parts of humectant, 0.4 part of pH regulator, 0.55 part of emollient and 90.65 parts of deionized water according to mass percentage. And mixing and stirring the components according to the method until the components are clear, so as to obtain the antibacterial additive solution for the wet tissue. At this time, the pH of the antimicrobial additive solution for wet tissues was 4.0.

Example 6

This example is a bacteriostasis experiment of the antiseptic additive solution for wet tissues prepared in examples 1-5.

(1) In the invention, common pathogenic bacteria in daily life, namely escherichia coli, staphylococcus aureus, pseudomonas aeruginosa and bacillus subtilis, are selected as experimental strains. Each strain was activated in broth at a shaker speed of 150rpm at 37 ℃ for 8 hours for use.

(2) The wet tissue antibacterial additive liquid products 1-5 sterilized at 121 ℃ for 20min are respectively added into a sterile broth culture medium, and the concentration is 0.05% (w/v). Meanwhile, deionized water with equal mass and sterilized at 121 ℃ for 20min is added into a broth culture medium without the wet tissue antibacterial additive solution product to serve as a control group for experiments.

(3) 1mL of activated bacteria solution (bacteria concentration 10) was taken under aseptic conditions⁹cfu/mL) was added to 49mL of the medium prepared in the above step (2), incubated at 37 ℃ and a shaker rotation speed of 150rpm for 6 hours, and then characterized by plate counting after dilution according to the national standard GB 4789.2-1994.

(4) The sterilization rate was calculated according to the following formula:

the sterilization rate is (blank group bacteria number-experimental group bacteria number)/blank group bacteria number

Wherein the number of bacteria is cfu/mL.

The results of the experiment are shown in table 1.

TABLE 1 Sterilization rates of different Care solutions against four bacteria

	Escherichia coli	Staphylococcus aureus	Pseudomonas aeruginosa	Bacillus subtilis
					Care solution 1	99.98％	99.83％	99.12％	99.80％
Care solution 2	75.28％	61.34％	66.02％	58.81％
					Care solution 3	95.21％	93.32％	98.04％	88.13％
Care solution 4	78.92％	70.13％	80.05％	69.90％
					Care solution 5	98.55％	91.28％	92.23％	94.39％

The result shows that the antibacterial additive solution product for the wet tissue has certain bactericidal effect on escherichia coli, staphylococcus aureus, pseudomonas aeruginosa and bacillus subtilis, wherein the nursing liquid prepared in the embodiment 1 has the best bactericidal effect.

In conclusion, the invention provides an antibacterial additive solution for wet tissues and a preparation method thereof, wherein the additive solution comprises the following components: antibacterial polypeptide, humectant, pH regulator, emollient and deionized water. The additive solution can effectively inhibit the breeding of common bacteria in daily life and reduce the stimulation to the skin. Meanwhile, the invention has simple formula and low cost, and is suitable for mass production.

The foregoing is only a preferred embodiment of the invention. Modifications thereof, which would occur to persons skilled in the art without departing from the principles of the invention, are also considered to be within the scope of the invention.

Claims

1. The antibacterial additive solution for the wet tissue is characterized by comprising the following components: the antibacterial skin-care product comprises an antibacterial polypeptide, a humectant, a pH regulator, an emollient and deionized water, wherein the sequence of the antibacterial polypeptide is LRKSKEKIGKEFKRRKDFLRNLV.

2. The antimicrobial additive solution for wet wipes of claim 1, wherein the humectant is glycerin.

3. The antiseptic additive solution for wet tissues as claimed in claim 1, wherein the pH regulator is citric acid.

4. The antiseptic additive for wet towel as recited in claim 1, wherein the emollient is polydimethylsiloxane.

5. The antibacterial additive solution for wet tissues as claimed in claim 1, wherein the composition comprises, in mass percent: 3 to 8 percent of antibacterial polypeptide, 1 to 1.5 percent of humectant, 0.1 to 0.5 percent of pH regulator, 0.4 to 0.6 percent of emollient and the balance of deionized water, wherein the pH value of the additive solution is 3.5 to 6.8.

6. The antibacterial additive solution for wet tissues as claimed in claim 5, wherein the composition comprises 6.2% of antibacterial polypeptide, 1.2% of humectant, 0.3% of pH regulator, 0.5% of emollient and the balance of deionized water by mass, and the pH value is 4.8.

7. The antibacterial additive solution for wet tissues as claimed in claim 6, characterized in that the preparation method comprises the following steps:

(1) weighing 3-8% of antibacterial polypeptide, 1-1.5% of humectant, 0.1-0.5% of pH regulator, 0.4-0.6% of emollient and the balance of deionized water according to mass percentage;

(2) mixing the antibacterial polypeptide, the humectant, the emollient and part of the pH regulator in the step (1) with deionized water, and uniformly stirring;

(3) further adjusting the pH value of the mixed solution to 3.5-6.8 by using a pH regulator;

8. The application of the antibacterial additive solution for the wet tissues as claimed in any one of claims 1 to 7 in the field of antibacterial wet tissues.