Chinese medicine composition and preparation method thereof for treating mycoplasma gallinarum
Technical field:
The invention belongs to veterinary drug technical fields, and in particular to a kind of for treating the Chinese medicine composition and its system of mycoplasma gallinarum
Preparation Method.
Background technique:
Mycoplasma gallinarum infection is to cause chicken and the chronic of turkey to exhale by chicken virus mycoplasma (MG) and Mycoplasma synoviae (MS)
Road transmission disease is inhaled, is mainly shown as that tracheitis and air bag are scorching in chicken, air bag inflammation and nasosinusitis is then shown as in turkey, can make sometimes
Conjunctivitis occurs for chick and poult.Mycoplasma gallisepticum infection can cause the chronic respiratory tract disease based on respiratory symptom
(chronic respiratory disease, CRD), it is characterized in that cough, stream nose liquid, respiratory tract rale and mouth breathing.Disease
Disease develops slowly, and the course of disease is long, and Adult Chicken is mostly subclinical infection, can be in chicken group's long-term existence and sprawling.
This disease is distributed in one of countries in the world, and the Important Infectious Diseases for endangering poultry husbandry.Infect this after being ill, young chicken growth
Bad, Adult Chicken egg drop reduction, air bag inflammation increases broiler carcass quality decline, discarded rate, and morbidity group's medication increases, and can cause
Serious economic loss.Chicken and turkey have neurological susceptibility to this disease, and 4~8 week old chickens and turkey are most sensitive, and standard-bred poultry is easier than hybrid chicken
Sense.Diseased chicken and subclinical infection chicken are the infections sources of this disease.This disease has vertically and horizontally two kinds of circulation ways.Pathogen can pass through disease
Through respiratory tract infection, contaminated drinking-water, feed, apparatus can also propagate this disease for chicken cough, the droplet of sneeze and dust.Upper breathing
Road and eye conjunctiva are the main portals of the MG invasion in aerosol or small droplet.Vertical transmission, which may make up, to be handed down from age to age, and makes this disease
In chicken group successively.But hatching egg spreading rate changeability is larger, and acute stage reaches as high as 50%, and the no clinical symptoms infection phase is only
It is 0.5%~5%.It has also been found that with the presence of pathogen in the sperm of infection cock, thus with it is middle when can also infect.
Environmental factor, which also plays, influences the popular important function of MG infection.The crowded propagation for exacerbating cause of disease of chicken group, simultaneously
It is crowded be one kind stress, reduce the resistance of chicken.Henhouse is dirty, excrement is put aside, and the content of ammonia increases stimulation breathing in air
Mucous membrane facilitates the development of mycoplasma.Environment temperature also has apparent influence to infection, at 7~10 DEG C, the air bag of infected chicken
5 times when scorching disease incidence is 31-32 DEG C, the seriousness of air bag inflammation is also greatly aggravated.Subalimentation will lead to mycoplasmal diseases
Occur, once had the shortage due to vitamin A and incurred the report that mycoplasma infection is broken out.
This disease is propagated more slowly in chicken group, but is propagated comparatively fast in the chicken of neopathy group.It is different according to environmental factor,
The severity of disease is widely different, as stocking density is big, sanitary condition is poor, climate change, coop ventilation are bad, ties up in feed
Raw element lacks and the chicken of different days mixes raising, can aggravate the seriousness of disease and case fatality rate is promoted to increase.There are many diseases
When pathogenic microorganism is secondary and is complicated by infection, this disease can be made more serious, wherein be mainly infectious bronchitis virus, infectious disease
Laryngotracheitis virus, newcastle disease virus, infectious bursal disease virus, secondary chicken poultry bacillus Escherichia coli etc..The chick of MG is carried,
In the attenuated vaccine immunity for carrying out the diseases such as newcastle disease, infective bronchitis and infectious laryngotracheitis with aerosol and nasal drip
Inoculation makes, and can lure the occurrence of this disease into.Attenuate vaccine is produced with the chicken embryo with mycoplasma, the pollution two of vaccine is easily caused to disseminate this
Disease.
This disease can occur throughout the year, popular serious with cold season.Chicken flock is more than Adult Chicken mass-sending disease.Adult Chicken
It is generally to distribute.When young age chicken falls ill, clinical symptoms are more typical, show as slurries or slurry viscosity nose liquid, make nostril blocking harm
Hinder breathing, shake the head again and again, sneeze, cough, sees also the gentle capsulitis of sinusitis, eye conjunctivitis.When inflammation spreads to lower respiratory tract,
It then breathes and coughs more significant, and have respiratory tract rale.Diseased chicken loss of appetite, growth retardation.To the later period, such as nasal cavity and socket of the eye
Exudate is accumulated in lower sinus then to cause to cause to blind like " goldfish eye " outside eyelid swelled, or even the prominent eyeball of accumulation object.Sometimes it closes
Save inflammation, walk lamely, but it is rare have can not stand.Occurs ataxia once in a while in turkey, this is because cause of disease invades brain
Caused by interior.Also some causes ascites symptom.Infected chicken all living creatures declines at performance.After clinical symptoms disappear, develop by difference
The inhibition of degree.Adult Chicken is seldom dead, and for young chicken such as without complication, case fatality rate is also low.After laying hen infection, there is fallopian tubal
Inflammation, only showing egg production decline and hatching rate reduces, and the chick viability hatched reduces.Such as secondary coli-infection, also go out
Existing anorexia and diarrhea, death and culling rate increase.
This bacterium has certain sensibility to Multiple Classes of Antibiotics, commonly use Tiamulin, tylosin, spectinomycin, streptomysin and
Erythromycin etc. has suitable curative effect to this disease.But antibiotic is easy to remain in meat, egg milk, causes food safety hazards.In order to
Make mycoplasma gallinarum obtain good prevention and control and solve by with antibiotic prophylaxis and treatment bring antibiotic residue hidden danger, the present invention
A kind of Chinese medicine composition and preparation method thereof for preventing and treating mycoplasma gallinarum has been invented according to Chinese medicine medical knowledge, chicken can be effectively controlled
Mycoplasma.
Summary of the invention:
For the symptom occurred after chicken infection mycoplasma, animal doctor debates that disease surely belongs to wind-cold affecting lung cough and asthma and nasal mucus is thin, lung
The deficiency of vital energy causes deficiency of yang weakened defensive QI, heat in Lung-Yin deficiency, and phlegm retention hinders lung, causes a large amount of white glutinous nasal mucus of nose stream, takes good care of oneself in addition accidentally, instead
The heresy of multiple exogenous disease, it is inside and outside to be harmonious.In sick long disease penetrates with, channels is obstructed, qi depression to blood stasis, and more nose key stops up, and causes eye
Eyelid swelling, obstinate refractory, there is arthritis in the later period, occurs walking lamely.Clinic distinguish disease be broadly divided into wind-cold affecting lung, phlegm retention resistance lung,
Three disease types of Lung Qi deficiency.
For antibiotic medicine, remaining defect and problem, the present invention provide one kind and are not easy remaining use easily in meat, egg
In the Chinese medicine composition and preparation method thereof for the treatment of mycoplasma gallinarum.
The present invention solves scheme used by its technical problem: it is a kind of for treating the Chinese medicine composition of mycoplasma gallinarum,
Including following raw material: 5-35 parts of ramulus cinnamomi, 5-30 parts of almond, 5-25 parts of the root of purple-flowered peucedanum, 5-25 parts of honeysuckle, 5-30 parts of Fructus Forsythiae, macleaya cordata
3-15 parts, 5-35 parts of the root of Dahurain angelica, 5-25 parts of the flower bud of lily magnolia, 5-20 parts of ginger, 5-20 parts of Mangnolia officinalis, 5-30 parts of the coptis, 5-25 parts of the tuber of pinellia.
It is above-mentioned a kind of for treating the Chinese medicine composition of mycoplasma gallinarum, including following raw material: 20-35 parts of ramulus cinnamomi, almond
20-30 parts, 20-25 parts of the root of purple-flowered peucedanum, 15-20 parts of honeysuckle, 15-25 parts of Fructus Forsythiae, 3-10 parts of macleaya cordata, 15-30 parts of the root of Dahurain angelica, the flower bud of lily magnolia
15-20 parts, 15-20 parts of ginger, 15-20 parts of Mangnolia officinalis, 15-25 parts of the coptis, 15-20 parts of the tuber of pinellia.
Our inducing sweat and dispelling exogenous evils, freeing lung and relieving asthma, dispelling cold and removing dampness.The affection of exogenous wind-cold table case of a physically strong patient running a high fever is cured mainly, exogenous disease holds wet exterior cold disease under the arm.Disease
See that fever with aversion to cold, lossless cough and asthma are trembled, skin and flesh is tightly hard, and tongue fur is thin white, floating and tense pulse, limbs pain, cough, white and greasy fur.We are pungent
Warm inducing diaphoresis, chill exterior disease are pathogenic cold hampering the exterior, and affection of exogenous wind-cold heresy is wet, and lung qi loses caused by a surname's drop.Suitable inducing sweat and dispelling exogenous evils are controlled, promoting the dispersing function of the lung is flat
Asthma, dispelling cold and removing dampness.Ramulus cinnamomi, honeysuckle pungent-warm in side, can inducing sweat and dispelling exogenous evils with dispersing wind and cold, and can declare benefit lung qi with cough of relievining asthma, the flower bud of lily magnolia,
Ginger dispels whole body rheumatism, shares using inducing diaphoresis eliminating evil as monarch drug in a prescription;Fructus Forsythiae, coptis sweating expelling pathogenic factors from muscles and skin, warming meridian, and limbs can be released
Pain is ministerial drug;Almond dispersing and lowering lung-QI is relieving cough and asthma, root of purple-flowered peucedanum ventilating the lung and relieving cough, macleaya cordata invigorating spleen, is adjutant;The root of Dahurain angelica, thickness
Mend, tuber of pinellia QI invigorating and in, coordinate all medicines, to make medicine.All medicines are harmonious, play altogether inducing sweat and dispelling exogenous evils, freeing lung and relieving asthma, eliminating cold to remove dampness the benefits of.
It is a kind of for treating the Chinese medicine composition of mycoplasma gallinarum, including following raw material: 5-20 parts of ginger;5-35 parts of radix scutellariae,
10-20 parts of Mangnolia officinalis, 5-30 parts of kuh-seng, 5-15 parts of macleaya cordata, 5-35 parts of radix bupleuri, 1-10 parts of honeysuckle, 5-30 parts of Fructus Forsythiae, Poria cocos 5-
30 parts.
It is above-mentioned a kind of for treating the Chinese medicine composition of mycoplasma gallinarum, including following raw material: 10-15 parts of ginger;Radix scutellariae
15-20 parts, 10-15 parts of Mangnolia officinalis, 20-25 parts of kuh-seng, 10-15 parts of macleaya cordata, 18-30 parts of radix bupleuri, 1-6 parts of honeysuckle, Fructus Forsythiae 10-
25 parts;10-20 parts of Poria cocos.
We are eliminating dampness and eliminating phlegm, and qi-regulating is in;Damp-phlegm syndrome is cured mainly, is vomitted, abdominal distension.Symptoms include coughing with a lot of sputum, color are white, tongue fur is white
Profit.Our disease is the dysfunction of the spleen in transport, damp evil cohesion, caused by mechanism of qi retardance.Control preferably it is eliminating dampness and eliminating phlegm, qi-regulating and in.Ginger eliminating dampness in side
Resolving sputum, stopping nausea and vomiting by lowering the adverse flow of QI are monarch drug in a prescription;Gas is dropped along then phlegm, and then phlegm disappears for gasification, therefore minister is with Poria cocos, Fructus Forsythiae regulating qi-flowing for eliminating phlegm;Again because phlegm is by wet
Raw, then wetization is transported in spleen rehabilitation, and wet to go, phlegm disappears, therefore is assistant with radix bupleuri, radix scutellariae, Mangnolia officinalis invigorating spleen to remove dampness tonifying Qi;Make with honeysuckle
Sharp lung qi is declared, macleaya cordata, kuh-seng invigorating spleen coordinate all medicines.All medicines share, have effects that eliminating dampness and eliminating phlegm, qi-regulating and in.
It is a kind of for treating the Chinese medicine composition of mycoplasma gallinarum, including following raw material: 5-20 parts of Radix Codonopsis, 7-15 parts of ginger,
5-25 parts of Radix Astragali, 1-10 parts of macleaya cordata, 5-15 parts of aster, 5-35 parts of Schisandra chinensis, 1-10 parts of Fructus Forsythiae, 2-10 parts of Mangnolia officinalis, honeysuckle
1-15 parts, 5-25 parts of Rehmannia glutinosa, 5-20 parts of the root bark of white mulberry.
It is above-mentioned a kind of for treating the Chinese medicine composition of mycoplasma gallinarum, including following raw material: 12-20 parts of Radix Codonopsis, ginger
7-10 parts, 16-25 parts of Radix Astragali, 1-5 parts of macleaya cordata, 5-11 parts of aster, 20-35 parts of Schisandra chinensis, 1-5 parts of Fructus Forsythiae, 2-5 parts of Mangnolia officinalis,
1-8 parts of honeysuckle, 12-25 parts of Rehmannia glutinosa, 8-20 parts of the root bark of white mulberry.
We cure mainly insufficiency of lung-QI,deficiency of lung-QI, the lungs cold of insufficiency type, cough with dyspnea, inverse Man Shangqi, and bored plug in pharynx loses heart, trembles with fear from shouldering, clearly
Cremate phlegm.Void is mostly using Radix Codonopsis as main ingredient, and Radix Codonopsis plays the role of strong and regulating gastointestinal, and righting prevents from invading in perverse trend in, can
Keep qi and blood vigorous, and strengthening vital QI to eliminate pathogenic factors, dispeling wind and dehumidification function, is monarch drug in a prescription;Radix Astragali clearing away the heat located at Shaoyang Channel it is hot and suffocating, in removing summer-heat heat, dredging it is stagnant
Gas, Radix Astragali bitter cold, hardship drop are let out the benefits of full, and the inverse and stomach function regulating of ginger heat-clearing drop, is ministerial drug;Aster, warm-natured, acrid flavour, hardship, under moistening lung
Gas, stopping coughing and removing phlegm, Schisandra chinensis invigorating the spleen to dry, astringing lung-QI drop is inverse, and the acid of Schisandra chinensis is received, the dissipation of convergence lung qi, Fructus Forsythiae warming meridian,
Mangnolia officinalis QI invigorating is in, macleaya cordata invigorating spleen, is adjutant;Glutinous rehmannia does, and trembles with fear, return heart, liver and kidney channel, Yin-nourishing and body fluid promoting, and honeysuckle is clear
Hot cool blood, the root bark of white mulberry have the effect of removing heat from lung and relieving asthma, inducing diuresis for removing edema, are usually used in dyspnea and cough due to lung-heat, and water drink stops lung, and turgor asthma is anxious, water
It is swollen, difficult urination, to make medicine.
The present invention also provides a kind of for treating the preparation method of the Chinese medicine composition of mycoplasma gallinarum, including following step
It is rapid:
(1) it weighs each raw material respectively in proportion, raw material is put into medicine decocting tank, 10 times of raw material weight amounts are added into tank
Pure water impregnates 1h;
(2) first time decoction is carried out after impregnating, is filtered after decocting 30min, is stayed and is decocted filtrate for the first time;To first
Secondary to decoct the secondary decoction of pure water progress that 10 times of amounts are added in resulting filter residue, filtering, which is stayed second, after decoction 30min decocts
Boil filtrate;
(3) merge and decoct gained filtrate twice, be condensed into medicinal extract, gained medicinal extract is dried, powder is sprayed into;
(4) filter residue is dried and is crushed, be uniformly mixed with the dry gained powder of filtrate, packing pack.
The present invention also provides a kind of for treating the preparation method of the Chinese medicine composition of mycoplasma gallinarum, including following step
It is rapid:
(1) it weighs each raw material respectively in proportion, raw material is put into medicine decocting tank, 10 times of raw material weight amounts are added into tank
70% ethyl alcohol impregnates 2h;
(2) first time decoction is carried out after impregnating, is filtered after decocting 45min, is stayed and is decocted filtrate for the first time;To first
Secondary to decoct the secondary decoction of 70% ethyl alcohol progress that 10 times of amounts are added in resulting filter residue, filtering is stayed second after decocting 45min
Decoct filtrate;
(3) merge and decoct gained filtrate twice, be condensed into medicinal extract, gained medicinal extract is dried, powder is sprayed into;
(4) filter residue is dried and is crushed, be uniformly mixed with the dry gained powder of filtrate, packing pack.
Beneficial effects of the present invention:
The Chinese medicine composition for the treatment of mycoplasma gallinarum of the present invention is made of that had between each component using pure traditional Chinese medicine raw material
There is good synergistic effect, can effectively treat mycoplasma gallinarum according to symptom Symptomatic medicine, which can be according to this
Various dosage forms in pharmacy are made in field conventional method, such as powder, granule, oral solution, paste, pill, tablet etc.;In and
Drug composition both can be used as drug use when specifically used, can also be used as additive use, can also be used as premix
Carrier;Mycoplasma gallinarum is treated using the present invention, both juice can also can be taken to drink water powder decocting with spice mixed feeding, it can be with
Gavage, and compared with antibiotic, Chinese medicine have it is pure natural, preventive treatment of disease, have no toxic side effect, noresidue, without drug resistance, thoroughly control
More the features such as, is to prevent and treat the choice drug of mycoplasma gallinarum, and preparation method is simple, is suitble to large-scale industrial metaplasia
The needs of production are suitable for promoting and applying in husbandry sector.
Specific embodiment:
Below with reference to embodiment, the present invention is further described.
Embodiment 1: a kind of for treating the Chinese medicine composition of mycoplasma gallinarum described in the present embodiment, the medicine is for treating chicken
Wind-cold affecting lung type illness caused by mycoplasma infection, including following raw material:
Ramulus cinnamomi 50g, almond 200g, root of purple-flowered peucedanum 200g, honeysuckle 250g, Fructus Forsythiae 50g, macleaya cordata 30g, root of Dahurain angelica 50g, the flower bud of lily magnolia
150g, ginger 200g, Mangnolia officinalis 200g, coptis 150g, tuber of pinellia 150g.
Preparation method: weighing above-mentioned raw materials respectively, respectively crushes all raw materials, crosses 400 meshes, is uniformly mixed,
Obtain compound Chinese medicinal preparation.
Embodiment 2: a kind of for treating the Chinese medicine composition of mycoplasma gallinarum described in the present embodiment, the medicine is for treating chicken
Wind-cold affecting lung type illness caused by mycoplasma infection, including following raw material: ramulus cinnamomi 350g, almond 50g, root of purple-flowered peucedanum 250g, honeysuckle
50g, Fructus Forsythiae 300g, macleaya cordata 100g, root of Dahurain angelica 350g, flower bud of lily magnolia 250g, ginger 50g, Mangnolia officinalis 50g, coptis 50g, tuber of pinellia 250g.
Preparation method: weighing above-mentioned raw materials respectively, and all raw materials are put into medicine decocting tank, and raw material weight 10 is added into tank
The pure water of amount impregnates 1h again, then carries out first time decoction, is filtered after decocting 30min, and first time is stayed to decoct filtrate;Institute
Second of decoction of pure water progress that filter residue adds 10 times of amounts is obtained, second of decoction filtrate is stayed in filtering after decoction 30min, merging
Then filtrate twice is condensed into medicinal extract, gained medicinal extract is dried in vacuo, powder is sprayed into;Gained filter residue is dried and is crushed,
It is uniformly mixed with the dry gained powder of filtrate, packing pack.
Embodiment 3: a kind of for treating the Chinese medicine composition of mycoplasma gallinarum described in the present embodiment, which uses
The wind-cold affecting lung type illness caused by treatment mycoplasma gallinarum infects, including following raw material: ramulus cinnamomi 200g, almond 300g, the root of purple-flowered peucedanum
50g, honeysuckle 150g, Fructus Forsythiae 150g, macleaya cordata 150g, root of Dahurain angelica 150g, flower bud of lily magnolia 50g, ginger 150g, Mangnolia officinalis 150g, the coptis
300g, tuber of pinellia 180g.
Preparation method: weighing above-mentioned raw materials respectively, and all raw materials are put into medicine decocting tank, and raw material weight 10 is added into tank
70% ethyl alcohol of amount impregnates 2h again, carries out first time decoction, decocts the first time decoction filtrate filtered after 45min;Filter residue adds again
70% ethyl alcohol for entering 10 times of amounts decocts 45min, obtains second of decoction filtrate: the filtrate decocted twice being mixed and is condensed into leaching
Then cream is dried, be sprayed into powder;Filter residue is dried and crushed, is uniformly mixed with the dry resulting powder of filtrate, packing dress
Bag.
Embodiment 4: a kind of for treating the Chinese medicine composition of mycoplasma gallinarum described in the present embodiment, the medicine is for treating chicken
Wind-cold affecting lung type illness caused by mycoplasma infection, including following raw material: ramulus cinnamomi 280g, almond 250g, root of purple-flowered peucedanum 230g, honeysuckle
180g, Fructus Forsythiae 200g, macleaya cordata 70g, root of Dahurain angelica 240g, flower bud of lily magnolia 170g, ginger 170g, Mangnolia officinalis 160g, coptis 200g, the tuber of pinellia
180g。
Preparation method: weighing above-mentioned raw materials respectively, and all raw materials are put into medicine decocting tank, and raw material weight 10 is added into tank
The pure water of amount impregnates 1h again, then carries out first time decoction, is filtered after decocting 30min, and first time is stayed to decoct filtrate;Institute
Second of decoction of pure water progress that filter residue adds 10 times of amounts is obtained, second of decoction filtrate is stayed in filtering after decoction 30min, merging
Then filtrate twice is condensed into medicinal extract, gained medicinal extract is dried in vacuo, powder is sprayed into;Gained filter residue is dried and is crushed,
It is uniformly mixed with the dry gained powder of filtrate, packing pack.
Embodiment 5: a kind of for treating the Chinese medicine composition of mycoplasma gallinarum described in the present embodiment, the medicine is for treating chicken
Wind-cold affecting lung type illness caused by mycoplasma infection, including following raw material: ramulus cinnamomi 200g, almond 50g, root of purple-flowered peucedanum 200g, honeysuckle
2000g, Fructus Forsythiae 250g, macleaya cordata 100g, root of Dahurain angelica 300g, flower bud of lily magnolia 200g, ginger 150g, Mangnolia officinalis 50g, coptis 250g, the tuber of pinellia
200g。
Preparation method: weighing above-mentioned raw materials respectively, and all raw materials are put into medicine decocting tank, and raw material weight 10 is added into tank
The pure water of amount impregnates 1h again, then carries out first time decoction, is filtered after decocting 30min, and first time is stayed to decoct filtrate;Institute
Second of decoction of pure water progress that filter residue adds 10 times of amounts is obtained, second of decoction filtrate is stayed in filtering after decoction 30min, merging
Then filtrate twice is condensed into medicinal extract, gained medicinal extract is dried in vacuo, powder is sprayed into;Gained filter residue is dried and is crushed,
It is uniformly mixed with the dry gained powder of filtrate, packing pack.
Embodiment 6: in order to verify in the illness of the present invention for treating wind-cold affecting lung caused by mycoplasma gallinarum
The beneficial effect of drug composition respectively carries out chicken virus mycoplasma and Mycoplasma synoviae using the Chinese medicine composition minimum antibacterial
Concentration (minimal inhibition concentration, MIC) and minimum bactericidal concentration (minimal
Bacteriocidal concentration, MBC) measurement, respectively most to one plant of reference culture and 13 plants of Clinical isolations
Low Mlc and minimum bactericidal concentration are tested.Test method is as follows:
(1) precision weighs ramulus cinnamomi 15g, almond 20g, root of purple-flowered peucedanum 5g, honeysuckle 15g, Fructus Forsythiae 20g, macleaya cordata 10g, the root of Dahurain angelica
15g, flower bud of lily magnolia 5g, ginger 15g, Mangnolia officinalis 15g, coptis 15g, tuber of pinellia 5g;Above-mentioned raw materials are mixed and are put into medicine decocting tank, are added into tank
The distilled water for entering 10 times of raw material weight amounts carries out first time decoction, uses instead after boiling and is cooked by slow fire 1h, remains during decoction
The water of 10 times of amounts, then with 4 layers of filtered through gauze;Residue add 6 times amount water according to first time decoct method continue decoct 1h it is laggard
Second of decoction filtrate of row filtering, merges filtrate twice, is concentrated under reduced pressure, makes its concentration 1g/ml.
(2) it prepares bacterium solution: chicken virus mycoplasma and Mycoplasma synoviae being seeded to culture medium, 37 DEG C of stationary cultures for 24 hours, are used
The blank cultures that sterilize correct bacterial concentration, make viable count 1-2 × 108CFU/ml, it is spare.
(3) minimum inhibitory concentration (minimal inhibition concentration, MIC) measures
It is carried out using test tube doubling dilution, determines Chinese medicine minimum inhibitory concentration (minimal inhibition
concentration,MIC).25, sterile test tube is taken to be numbered, every pipe adds culture medium 2mL, in the first pipe in addition
Medical fluid 2mL takes out 2mL and is put into the second pipe, and so on, it is discarded until the 23rd pipe takes out 2.0ml;Medical fluid is not added in 24th pipe, makees
For positive control, the 25th pipe takes out 2.0ml and discards, be not added as negative control, the 25th pipe plus tested medical fluid 2.0ml after mixing
Bacterium respectively manages parallel 3 parts as negative control.I.e. the concentration of drug is (1g/mL, 500mg/mL, 250mg/mL, 125mg/
mL、62.5mg/mL、31.25mg/mL、15.63mg/mL、7.81mg/mL、3.95mg/mL、1.98mg/mL、0.99mg/mL、
0.49mg/mL、0.25mg/mL、0.12mg/mL、0.06mg/mL、0.03mg/mL、15.43μg/mL、7.71μg/mL、3.86μ
G/mL, 1.93 μ g/mL, 0.97 μ g/mL, 0.48 μ g/mL, 0.24 μ g/mL), 10 μ L (10 of bacterium solution is added in 1~24 pipe8CFU/
ML), every kind of medicine does 3 and takes its average value in parallel, be placed in 37 DEG C of constant incubators and cultivate 24-48h, observes result.Sterile life
Long region is the smallest Mlc (MIC) of the medicine.
(4) minimum bactericidal concentration (minimal bacteriocidal concentration, MBC) measures
Culture in each dilution tube for more than MIC endpoint concentration not growing bacterium is inoculated with aseptic cotton carrier streaking inoculation
It cultivates for 24 hours, is observed as a result, 3 tests are averaged on culture medium, being placed in 37 DEG C of constant incubators.
(5) 1 and table 2 be the results are shown in Table
A kind of Chinese prescription for treating wind-cold affecting lung symptom caused by mycoplasma gallinarum infects of table 1 tests chicken virus mycoplasma
As a result
A kind of Chinese medicine composition for treating wind-cold affecting lung symptom caused by mycoplasma gallinarum infects of table 2 is to Mycoplasma synoviae
Test result
By Tables 1 and 2 it is found that the Chinese traditional medicine composition of the present invention for the wind-cold affecting lung illness treating mycoplasma gallinarum
Object has preferable antibacterial and bactericidal effect to chicken virus mycoplasma and Mycoplasma synoviae.
Embodiment 7: a kind of for treating the Chinese medicine composition of mycoplasma gallinarum described in the present embodiment, the medicine is for treating chicken
Phlegm retention caused by mycoplasma infection hinders pulmonary type symptom, including following raw material:
Ginger 200g, radix scutellariae 200g, Mangnolia officinalis 150g, kuh-seng 50g, macleaya cordata 100g, radix bupleuri 350g, honeysuckle 10g, Fructus Forsythiae
50g, Poria cocos 50g.
Preparation method: weighing above-mentioned each raw material respectively, all raw materials crushed respectively, crosses 400 meshes, and mixing is equal
It is even, obtain compound Chinese medicinal preparation.
Embodiment 8: a kind of for treating the Chinese medicine composition of mycoplasma gallinarum described in the present embodiment, the medicine is for treating chicken
Phlegm retention caused by mycoplasma infection hinders pulmonary type symptom, including following raw material: ginger 50g, radix scutellariae 350g, Mangnolia officinalis 100g, kuh-seng
300g, macleaya cordata 50g, radix bupleuri 50g, honeysuckle 60g, Fructus Forsythiae 100g, Poria cocos 300g.
Preparation method: weighing above-mentioned raw materials respectively, and all raw materials are mixed and are put into medicine decocting tank, and raw material weight is added into tank
The pure water for measuring 10 times of amounts impregnates 1h, then carries out first time decoction, is filtered after decocting 30min, stays and decocts filter for the first time
Liquid;The pure water that gained filter residue adds 10 times of amounts carries out second and decocts, and filtering stays second of decoction to filter after decocting 30min
Second of filtrate is mixed and then is condensed into medicinal extract with first time filtrate, gained medicinal extract is dried in vacuo, powder is sprayed by liquid
End;Gained filter residue is dried and crushed, is uniformly mixed with the dry gained powder of filtrate, packing pack.
Embodiment 9: a kind of for treating the Chinese medicine composition of mycoplasma gallinarum described in the present embodiment, the medicine is for treating chicken
Phlegm retention caused by mycoplasma infection hinders pulmonary type symptom, including following raw material: ginger 120g, radix scutellariae 170g, Mangnolia officinalis 130g, kuh-seng
240g, macleaya cordata 130g, radix bupleuri 240g, honeysuckle 30g, Fructus Forsythiae 180g, Poria cocos 160g.
Preparation method: weighing above-mentioned raw materials respectively, and all raw materials are mixed and are put into medicine decocting tank, and raw material weight is added into tank
The pure water for measuring 10 times of amounts impregnates 1h, then carries out first time decoction, is filtered after decocting 30min, stays and decocts filter for the first time
Liquid;The pure water that gained filter residue adds 10 times of amounts carries out second and decocts, and filtering stays second of decoction to filter after decocting 30min
Second of filtrate is mixed and then is condensed into medicinal extract with first time filtrate, gained medicinal extract is dried in vacuo, powder is sprayed by liquid
End;Gained filter residue is dried and crushed, is uniformly mixed with the dry gained powder of filtrate, packing pack.
Embodiment 10: a kind of for treating the Chinese medicine composition of mycoplasma gallinarum described in the present embodiment, the medicine is for treating
Phlegm retention caused by mycoplasma gallinarum infects hinders pulmonary type symptom, including following raw material:
Ginger 100g, radix scutellariae 50g, Mangnolia officinalis 200g, kuh-seng 200g, macleaya cordata 150g, radix bupleuri 180g, honeysuckle 100g, even
Stick up 300g, Poria cocos 100g.
Preparation method: weighing above-mentioned raw materials respectively, and all raw materials are put into medicine decocting tank, and raw material weight 10 is added into tank
70% ethyl alcohol of amount impregnates 2h again, carries out first time decoction, decocts the first time decoction filtrate filtered after 45min;Filter residue adds again
70% ethyl alcohol for entering 10 times of amounts decocts 45min, obtains second of decoction filtrate: the filtrate decocted twice being mixed and is condensed into leaching
Then cream is dried, be sprayed into powder;Filter residue is dried and crushed, is uniformly mixed with the dry resulting powder of filtrate, packing dress
Bag.
Embodiment 11: a kind of for treating the Chinese medicine composition of mycoplasma gallinarum described in the present embodiment, the medicine is for treating
Phlegm retention caused by mycoplasma gallinarum infects hinders pulmonary type symptom, including following raw material:
Ginger 150g, radix scutellariae 150g, Mangnolia officinalis 200g, kuh-seng 250g, macleaya cordata 150g, radix bupleuri 300g, honeysuckle 100g, even
Stick up 250g, Poria cocos 200g.
Preparation method: weighing above-mentioned raw materials respectively, and all raw materials are put into medicine decocting tank, and raw material weight 10 is added into tank
70% ethyl alcohol of amount impregnates 2h again, carries out first time decoction, decocts the first time decoction filtrate filtered after 45min;Filter residue adds again
70% ethyl alcohol for entering 10 times of amounts decocts 45min, obtains second of decoction filtrate: the filtrate decocted twice being mixed and is condensed into leaching
Then cream is dried, be sprayed into powder;Filter residue is dried and crushed, is uniformly mixed with the dry resulting powder of filtrate, packing dress
Bag.
Embodiment 12: of the present invention for treating the Chinese medicine of the resistance lung disorder of phlegm retention caused by mycoplasma gallinarum in order to verify
The beneficial effect of composition respectively carries out chicken virus mycoplasma and Mycoplasma synoviae using the Chinese medicine composition minimum antibacterial dense
Spend (minimal inhibition concentration, MIC) and minimum bactericidal concentration (minimal bacteriocidal
Concentration, MBC) measurement, the minimum inhibitory concentration to one plant of reference culture and 13 plants of Clinical isolations and most respectively
Low bacteriocidal concentration is tested.Test method is as follows:
(1) ginger 15g, radix scutellariae 15g, Mangnolia officinalis 10g, kuh-seng 20g, macleaya cordata 10g, radix bupleuri 18g, honeysuckle are accurately weighed
6g, Fructus Forsythiae 10g, Poria cocos 20g;Above-mentioned raw materials are put into medicine decocting tank, into tank be added 10 times of raw material weight amount distilled water into
Row decocts for the first time, uses instead after boiling and is cooked by slow fire 1h, the water of 10 times of amounts is remained during decoction, then with 4 layers of gauze mistake
Filter;Residue adds 6 times of amount water to continue to decoct second of decoction filtrate being filtered after 1h according to the method that first time decocts, and merges
Filtrate twice is concentrated under reduced pressure, makes its concentration 1g/ml.
(2) it prepares bacterium solution: chicken virus mycoplasma and Mycoplasma synoviae being seeded to culture medium, 37 DEG C of stationary cultures for 24 hours, are used
The blank cultures that sterilize correct bacterial concentration, make viable count 1-2 × 108CFU/ml, it is spare.
(3) minimum inhibitory concentration (minimal inhibition concentration, MIC) measures
It is carried out using test tube doubling dilution, determines Chinese medicine minimum inhibitory concentration (minimal inhibition
concentration,MIC).25, sterile test tube is taken to be numbered, every pipe adds culture medium 2mL, in the first pipe in addition
Medical fluid 2mL takes out 2mL and is put into the second pipe, and so on, it is discarded until the 23rd pipe takes out 2.0ml;Medical fluid is not added in 24th pipe, makees
For positive control, the 25th pipe takes out 2.0ml and discards, be not added as negative control, the 25th pipe plus tested medical fluid 2.0ml after mixing
Bacterium respectively manages parallel 3 parts as negative control.I.e. the concentration of drug is (1g/mL, 500mg/mL, 250mg/mL, 125mg/
mL、62.5mg/mL、31.25mg/mL、15.63mg/mL、7.81mg/mL、3.95mg/mL、1.98mg/mL、0.99mg/mL、
0.49mg/mL、0.25mg/mL、0.12mg/mL、0.06mg/mL、0.03mg/mL、15.43μg/mL、7.71μg/mL、3.86μ
G/mL, 1.93 μ g/mL, 0.97 μ g/mL, 0.48 μ g/mL, 0.24 μ g/mL), 10 μ L (10 of bacterium solution is added in 1~24 pipe8CFU/
ML), every kind of medicine does 3 and takes its average value in parallel, be placed in 37 DEG C of constant incubators and cultivate 24-48h, observes result.Sterile life
Long region is the smallest Mlc (MIC) of the medicine.
(4) minimum bactericidal concentration (minimal bacteriocidal concentration, MBC) measures
Culture in each dilution tube for more than MIC endpoint concentration not growing bacterium is inoculated with aseptic cotton carrier streaking inoculation
It cultivates for 24 hours, is observed as a result, 3 tests are averaged on culture medium, being placed in 37 DEG C of constant incubators.
(5) 3 and table 4 be the results are shown in Table
A kind of Chinese medicine composition for treating phlegm retention resistance lung symptom caused by mycoplasma gallinarum infects of table 3 tries chicken virus mycoplasma
Test result
A kind of Chinese medicine composition for treating phlegm retention resistance lung symptom caused by mycoplasma gallinarum infects of table 4 is to Mycoplasma synoviae
Test result
It is by table 3 and table 4 it is found that of the present invention for treating in phlegm retention resistance lung symptom caused by mycoplasma gallinarum infects
Drug composition has preferable antibacterial and bactericidal effect to chicken virus mycoplasma and Mycoplasma synoviae.
Embodiment 13: a kind of for treating the Chinese medicine composition of mycoplasma gallinarum described in the present embodiment, the medicine is for treating
Lung qi deficiency symptom caused by mycoplasma gallinarum infects, including following raw material: Radix Codonopsis 200g, ginger 70g, Radix Astragali 160g, macleaya cordata
50g, aster 50g, Schisandra chinensis 50g, Fructus Forsythiae 10g, Mangnolia officinalis 100g, honeysuckle 10g, Rehmannia glutinosa 250g, root bark of white mulberry 80g.
Preparation method: weighing above-mentioned raw materials respectively, respectively crushes all raw materials, crosses 400 meshes, is uniformly mixed,
Obtain compound Chinese medicinal preparation.
Embodiment 14: a kind of for treating the Chinese medicine composition of mycoplasma gallinarum described in the present embodiment, the medicine is for treating
Lung qi deficiency symptom caused by mycoplasma gallinarum infects, including following raw material:
Radix Codonopsis 50g, ginger 150g, Radix Astragali 250g, macleaya cordata 100g, aster 110g, Schisandra chinensis 200g, Fructus Forsythiae 50g, Mangnolia officinalis
20g, honeysuckle 80g, Rehmannia glutinosa 120g, root bark of white mulberry 200g.
Preparation method: weighing above-mentioned raw materials respectively, and all raw materials are put into medicine decocting tank, adds addition raw material weight 10 in tank
The pure water of amount impregnates 1h again, then carries out first time decoction, is filtered after decocting 30min, and first time is stayed to decoct filtrate;Institute
Filter residue adds the pure water of 10 times of amounts and carries out second and decoct, second of decoction filtrate is stayed in filtering after decocting 30min, by the
Secondary filtrate mixes and then is condensed into medicinal extract with first time filtrate, and gained medicinal extract is dried in vacuo, powder is sprayed into;By gained
Filter residue is dried and is crushed, and is uniformly mixed with the dry gained powder of filtrate, packing pack.
Embodiment 15: a kind of for treating the Chinese medicine composition of mycoplasma gallinarum described in the present embodiment, the medicine is for treating
Lung qi deficiency symptom caused by mycoplasma gallinarum infects, including following raw material:
Radix Codonopsis 160g, ginger 85g, Radix Astragali 200g, macleaya cordata 30g, aster 80g, Schisandra chinensis 280g, Fructus Forsythiae 30g, Mangnolia officinalis
30g, honeysuckle 50g, Rehmannia glutinosa 200g, root bark of white mulberry 150g.
Preparation method: weighing above-mentioned raw materials respectively, and all raw materials are put into medicine decocting tank, adds addition raw material weight 10 in tank
The pure water of amount impregnates 1h again, then carries out first time decoction, is filtered after decocting 30min, and first time is stayed to decoct filtrate;Institute
Filter residue adds the pure water of 10 times of amounts and carries out second and decoct, second of decoction filtrate is stayed in filtering after decocting 30min, by the
Secondary filtrate mixes and then is condensed into medicinal extract with first time filtrate, and gained medicinal extract is dried in vacuo, powder is sprayed into;By gained
Filter residue is dried and is crushed, and is uniformly mixed with the dry gained powder of filtrate, packing pack.
Embodiment 16: a kind of for treating the Chinese medicine composition of mycoplasma gallinarum described in the present embodiment, the medicine is for treating
Lung qi deficiency symptom caused by mycoplasma gallinarum infects, including following raw material:
Radix Codonopsis 120g, ginger 100g, Radix Astragali 50g, macleaya cordata 10g, aster 150g, Schisandra chinensis 350g, Fructus Forsythiae 100g, Mangnolia officinalis
50g, honeysuckle 150g, Rehmannia glutinosa 50g, root bark of white mulberry 50g.
Preparation method: weighing above-mentioned raw materials respectively, and all raw materials are put into medicine decocting tank, and 10 times of amounts are added into tank
70% ethyl alcohol impregnates 2h, carries out first time decoction, decocts the first time decoction filtrate filtered after 45min;Filter residue adds 10 times
70% ethyl alcohol of amount decocts 45min, obtains second of decoction filtrate: the filtrate decocted twice being mixed and is condensed into medicinal extract, so
After be dried, be sprayed into powder;Filter residue is dried and crushed, is uniformly mixed with the dry resulting powder of filtrate, packing pack.
Embodiment 17: of the present invention for treating the Chinese medicine group of Lung Qi deficiency illness caused by mycoplasma gallinarum in order to verify
The beneficial effect for closing object carries out minimum inhibitory concentration to chicken virus mycoplasma and Mycoplasma synoviae respectively using the Chinese medicine composition
(minimal inhibition concentration, MIC) and minimum bactericidal concentration (minimal bacteriocidal
Concentration, MBC) measurement, the minimum inhibitory concentration to one plant of reference culture and 13 plants of Clinical isolations and most respectively
Low bacteriocidal concentration is tested.Test method is as follows:
(1) Radix Codonopsis 12g, ginger 10g, Radix Astragali 16g, macleaya cordata 5g, aster 10g, Schisandra chinensis 20g, Fructus Forsythiae 5g accurately are weighed,
Mangnolia officinalis 5g, honeysuckle 8g, Rehmannia glutinosa 10g, root bark of white mulberry 8g;All raw materials are put into medicine decocting tank, raw material weight is added into tank
The distilled water of 10 times of amounts carries out first time decoction, uses instead after boiling and is cooked by slow fire 1h, and the water of 10 times of amounts is remained during decoction,
Then with 4 layers of filtered through gauze;Residue add 6 times amount water according to first time decoct method continue decoct 1h after be filtered second
Secondary decoction filtrate merges filtrate twice, is concentrated under reduced pressure, makes its concentration 1g/ml.
(2) it prepares bacterium solution: chicken virus mycoplasma and Mycoplasma synoviae being seeded to culture medium, 37 DEG C of stationary cultures for 24 hours, are used
The blank cultures that sterilize correct bacterial concentration, make viable count 1-2 × 108CFU/ml, it is spare.
(3) minimum inhibitory concentration (minimal inhibition concentration, MIC) measures
It is carried out using test tube doubling dilution, determines Chinese medicine minimum inhibitory concentration (minimal inhibition
concentration,MIC).25, sterile test tube is taken to be numbered, every pipe adds culture medium 2mL, in the first pipe in addition
Medical fluid 2mL takes out 2mL and is put into the second pipe, and so on, it is discarded until the 23rd pipe takes out 2.0ml;Medical fluid is not added in 24th pipe, makees
For positive control, the 25th pipe takes out 2.0ml and discards, be not added as negative control, the 25th pipe plus tested medical fluid 2.0ml after mixing
Bacterium respectively manages parallel 3 parts as negative control.I.e. the concentration of drug is (1g/mL, 500mg/mL, 250mg/mL, 125mg/
mL、62.5mg/mL、31.25mg/mL、15.63mg/mL、7.81mg/mL、3.95mg/mL、1.98mg/mL、0.99mg/mL、
0.49mg/mL、0.25mg/mL、0.12mg/mL、0.06mg/mL、0.03mg/mL、15.43μg/mL、7.71μg/mL、3.86μ
G/mL, 1.93 μ g/mL, 0.97 μ g/mL, 0.48 μ g/mL, 0.24 μ g/mL), 10 μ L (10 of bacterium solution is added in 1~24 pipe8CFU/
ML), every group do 3 it is parallel, take its average value, be placed in 37 DEG C of constant incubators and cultivate 24-48h, observe result.Asepsis growth
Region be the medicine the smallest Mlc (MIC).
(4) minimum bactericidal concentration (minimal bacteriocidal concentration, MBC) measures
Culture in each dilution tube for more than MIC endpoint concentration not growing bacterium is inoculated with aseptic cotton carrier streaking inoculation
It cultivates for 24 hours, is observed as a result, 3 tests are averaged on culture medium, being placed in 37 DEG C of constant incubators.
(5) 5 and table 6 be the results are shown in Table
A kind of Chinese medicine composition for treating Lung Qi deficiency symptom caused by mycoplasma gallinarum infects of table 5 tests chicken virus mycoplasma
As a result
A kind of Chinese medicine composition for treating Lung Qi deficiency symptom caused by mycoplasma gallinarum infects of table 6 tries Mycoplasma synoviae
Test result
By table 5 and table 6 it is found that the Chinese medicine group of wind-cold affecting lung symptom caused by treatment mycoplasma gallinarum of the present invention infects
Closing object has preferable antibacterial and bactericidal effect to chicken virus mycoplasma and Mycoplasma synoviae.
Embodiment 18: clinical application effect
There are 10,000 laying hens at case 1:2016 September 11 days, certain poultry family of breaking a seal, cough individually, breathing has hello
Sound, diseased chicken increases after 2d, and diseased chicken eyelid swelled has a head swelling;There is slurries sexual secretion in nasal cavity, chicken, which gets rid of head, secretion
Outflow, night can hear the symptoms such as sound of stridulating in henhouse, dead more than 60.Clinical symptoms, diseased chicken initial stage flow serosity or stickiness
Nose liquid, sneezing.Inflammation spreads to lower respiratory tract therewith, and appearance cough, expiratory dyspnea, tracheae have rale, loss of appetite, syntexis
With egg production decline etc..It is diagnosed as mycoplasma gallinarum through pathogen separation, identification, wind-cold affecting lung and phlegm retention hinder pulmonary type.It is used with the present invention
The Chinese medicine composition 1kg of the wind-cold affecting lung symptom caused by treatment mycoplasma gallinarum infects is watered 5 tons, twice a day, 2d is used in conjunction, allows
Chicken is freely drunk;Then the present invention is converted for treating the Chinese medicine composition 1kg of phlegm retention resistance lung symptom caused by mycoplasma gallinarum infects
5 tons of water, twice a day, 2d is used in conjunction, chicken is allowed freely to drink;The 5th day state of an illness of medication is controlled, and egg production bottom out is to just
Often.
Case 2:2017 March 6,7000 55 age in days chickens morbidity of table mountain chicken house.Field observation finds diseased chicken
Appetite stimulator has a running nose, gets rid of nose, sheds tears, coughing, breathing rale, is diagnosed as wind-cold affecting lung and lung qi deficiency.It is used with the present invention
The Chinese medicine composition 1kg of the wind-cold affecting lung symptom caused by treatment mycoplasma gallinarum infects is watered 5 tons, twice a day, 2d is used in conjunction, allows
Chicken is freely drunk;Then it is converted with the present invention for treating the Chinese medicine composition 1kg of Lung Qi deficiency symptom caused by mycoplasma gallinarum infects
5 tons of water, twice a day, 2d is used in conjunction, chicken is allowed freely to drink;The 5th day state of an illness of medication is controlled, and symptom disappears after 5 days, and chicken group is extensive
It is multiple normal.
Case 3:2018 April 17, the 13000 73 age in days chickens morbidities of Shangqiu chicken house, on inspection diseased chicken cough, gas
Asthma, tracheae have rale, are diagnosed as wind-cold affecting lung and phlegm retention resistance pulmonary type.With the present invention for treating wind caused by mycoplasma gallinarum infects
The Chinese medicine composition 1kg of cold beam lung symptom is watered 5 tons, twice a day, 2d is used in conjunction, chicken is allowed freely to drink;Then of the invention with using
Chinese medicine composition 1kg for treating phlegm retention resistance lung symptom caused by mycoplasma gallinarum infects is watered 5 tons, twice a day, 2d is used in conjunction,
Chicken is allowed freely to drink;Symptom disappears after 4 days, and chicken group restores normal.
The above be preferred embodiments of the invention, all equivalent variationss done according to scope of the present invention patent with repair
Decorations, all should belong to protection scope of the present invention.