CN109392684B - Dynamic observation device for growth of wetland plant clone organ and use method thereof - Google Patents
Dynamic observation device for growth of wetland plant clone organ and use method thereof Download PDFInfo
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- CN109392684B CN109392684B CN201811242168.8A CN201811242168A CN109392684B CN 109392684 B CN109392684 B CN 109392684B CN 201811242168 A CN201811242168 A CN 201811242168A CN 109392684 B CN109392684 B CN 109392684B
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- 210000000056 organ Anatomy 0.000 title claims abstract description 25
- 230000012010 growth Effects 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 title claims abstract description 9
- 238000009423 ventilation Methods 0.000 claims abstract description 44
- 238000005286 illumination Methods 0.000 claims abstract description 16
- 238000007789 sealing Methods 0.000 claims description 53
- 238000004891 communication Methods 0.000 claims description 21
- 239000008399 tap water Substances 0.000 claims description 21
- 235000020679 tap water Nutrition 0.000 claims description 21
- 230000007704 transition Effects 0.000 claims description 12
- 230000003287 optical effect Effects 0.000 claims description 11
- 230000008635 plant growth Effects 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 2
- 239000003818 cinder Substances 0.000 claims 2
- 238000012258 culturing Methods 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 35
- 239000012535 impurity Substances 0.000 description 4
- 239000002893 slag Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000010367 cloning Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- 244000261422 Lysimachia clethroides Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000021368 organ growth Effects 0.000 description 1
- 230000010254 physiological adaptation Effects 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The dynamic observation device for growth of the wetland plant cloned organ and the application method are used for conveniently culturing and observing the cloned plant organ. The automatic culture device comprises an upper cover, a culture bottle, a support rod, an illumination mechanism, a ventilation mechanism and a distribution mechanism, wherein the upper cover is arranged on the culture bottle, a measuring scale which is vertically arranged is arranged in the culture bottle, the support rod is detachably arranged on the side wall of the upper cover, a soft rod is fixed at the top of the support rod, and the illumination mechanism with adjustable illumination intensity is arranged on the soft rod; the top of the upper cover is provided with a ventilation mechanism, and the air exchange between the culture bottle and the outside is realized through the ventilation mechanism. The outer wall of the upper cover is fixedly provided with a connecting pipe, the connecting pipe is provided with a distribution mechanism, the distribution mechanism comprises a shell, a regulator, a first conduit, a second conduit, a mixing pipe, a fixed shaft and a handle, the outer wall of the shell is provided with the first conduit, the second conduit and the mixing pipe, and the regulator is rotatably arranged in the inner cavity of the shell. The invention can conveniently realize the growth observation of cloned plant organs.
Description
Technical Field
The invention relates to the technical field of cloning plant growth observation, in particular to a dynamic observation device for growth of a wetland plant cloning organ and a use method thereof.
Background
The plant clone organ is a vector for realizing population growth of clone plants through asexual propagation, and comprises tissue organs such as buds, tillers or branches connected with a parent plant. Plant clone organ growth status is an important way for displaying individual physiological adaptation strategy and stress response mechanism of plants. At present, research on plant clone organs is generally carried out by collecting plant individuals and tissue organs for observation and measurement, and the observation mode of collecting plant individuals or tissue organs not only damages clone plants, but also causes resource waste, which is unfavorable for long-term dynamic observation, because the stress resistance and the anti-interference capability of the plant clone organs are weak. Therefore, a dynamic observation device for growth of a wetland plant clone organ is needed urgently, and is used for realizing in-situ nondestructive observation and sustainable dynamic research on the plant clone organ, and the device is convenient to operate, accurate in observation and free of damage to plants.
Disclosure of Invention
The invention aims to provide a dynamic observation device for growth of a cloned plant organ of a wetland and a use method thereof, which are used for conveniently culturing and observing the cloned plant organ.
The technical scheme adopted for solving the technical problems is as follows: the dynamic observation device for growth of the wetland plant clone organ is characterized by comprising an upper cover, a culture bottle, a support rod, an illumination mechanism, a ventilation mechanism and a distribution mechanism, wherein the upper cover is arranged on the bottle mouth of the culture bottle in a threaded manner, a round hole for placing plant stems is formed in the center of the upper cover, a measuring scale which is vertically placed in the culture bottle is arranged in the culture bottle, the support rod is detachably arranged on the side wall of the upper cover, a soft rod is fixed at the top of the support rod, and the illumination mechanism with adjustable illumination intensity is arranged on the soft rod;
the top of the upper cover is provided with a ventilation mechanism, the ventilation mechanism comprises a ventilation box and a ventilation pipe, the ventilation box comprises an upper plate, a telescopic pipe and a lower plate which are sequentially arranged from top to bottom, the lower plate is in threaded connection with the top of the upper cover, the lower plate and the top of the upper cover are well sealed, the telescopic pipe comprises two layers which are internally and externally arranged, the telescopic pipe, the upper plate, the telescopic pipe and the lower plate are well sealed, and an air cavity is formed between the inner layer and the outer layer of the telescopic pipe in a surrounding manner; an upper communication hole is formed in the lower plate, a lower communication hole communicated with the upper communication hole is formed in the top of the upper cover, an air exchanging pipe communicated with the air cavity is fixed at the top of the upper plate, a pair of baffles which are arranged up and down are detachably arranged on the inner side of the air exchanging pipe, a sealing ring fixedly connected with the inner wall of the air exchanging pipe is arranged between the two baffles, a sealing plug is arranged between the two baffles, a sealing spring is arranged between the sealing plug and the baffles, and the sealing plug is tightly contacted with the inner wall of the sealing ring under the action of the two sealing springs; an upper avoidance hole is formed in the center of the upper plate, a lower avoidance hole is formed in the center of the lower plate, and the round hole, the upper avoidance hole and the lower avoidance hole cooperate to clamp stems of plants;
the utility model provides a be fixed with the connecting pipe on the outer wall of upper cover, be equipped with dispensing mechanism on the connecting pipe, dispensing mechanism includes shell, regulator, first pipe, second pipe, mixing tube, fixed axle and handle, shell and regulator are cylindrical hollow structure, be equipped with first pipe, second pipe and the mixing tube that set gradually along clockwise on the outer wall of shell, the regulator rotates the outer wall and the shell inner wall in close contact of installing in the shell inner chamber and regulator, be equipped with along first entry that corresponds of first pipe, the second entry that corresponds with the second pipe and the export that corresponds with the mixing tube that set gradually along clockwise on the lateral wall of regulator, be fixed with the fixed axle on the axis of regulator, stretch out the shell one section and be fixed with the handle on the fixed axle after the one end of fixed axle passes the shell.
Further, a pair of slots are formed in the inner wall of the round hole, clamping plates are movably arranged in the slots, clamping springs are arranged between the clamping plates and the inner wall of the slots, and the two clamping plates are contacted and enclose a clamping hole under the action of the clamping springs.
Further, the top of upper cover is equipped with inside and outside first inner ring and the first outer loop that sets up, is equipped with inside and outside second inner ring and the second outer loop that sets up in the bottom of hypoplastron, second outer loop and first outer loop threaded connection, second inner ring and first inner ring threaded connection, and first inner ring, second inner ring, first outer loop and second outer loop set gradually from inside to outside.
Further, a track seat is fixed on the baffle, guide rods are respectively fixed at the top and the bottom of the sealing plug, the guide rods are in sliding connection with the corresponding track seats, and a sealing spring is arranged between the track seat and the sealing plug.
Further, the device also comprises a purifying mechanism, wherein the purifying mechanism comprises a U-shaped filter in threaded connection with the second guide pipe and a filter screen arranged inside two ends of the filter, a slag discharging port is arranged at the bottom of the filter, and a plug is installed on the slag discharging port in a threaded manner.
Further, the lighting mechanism comprises a lamp box fixed on the soft rod, a lighting lamp arranged in the lamp box, a bracket fixed on the lamp box, a rotating plate rotatably arranged in the bracket, a top plate arranged below the rotating plate, a tensioning spring arranged between the top plate and the rotating plate, a vertical shaft fixed at the bottom of the top plate, a plurality of optical filters arranged around the vertical shaft and a connecting rod arranged between the optical filters and the vertical shaft, wherein the optical transmission of each optical filter is different.
Further, an outer rod is fixed at the bottom of the rotating plate, an inner rod is fixed at the top of the top plate, and the upper part of the inner rod is arranged in the inner cavity of the outer rod and is in sliding connection with the inner rod.
The application method of the dynamic observation device for growth of the wetland plant clone organ is characterized by comprising the following steps:
(1) Placing stems of cloned plants in the clamping holes of the upper cover, and installing an air ventilation box at the top of the upper cover;
(2) Adding culture solution into a culture solution barrel, connecting a transition pipe with a tap water pipe, adding tap water into the transition pipe, and adjusting the ratio of the culture solution to tap water entering a culture bottle according to the requirement;
(3) Turning on an illuminating lamp according to the requirement, and adjusting the brightness of the irradiated clone plants;
(4) The ventilation box is pressed as required to allow the flask to exchange gas with the outside.
The beneficial effects of the invention are as follows: the dynamic observation device for growth of the wetland plant clone organ and the use method thereof can realize the cultivation of the clone plant; the distribution mechanism is arranged, so that the required mixed liquid can be added into the culture flask according to the requirement; the illumination mechanism can provide illumination with required intensity for the growth of the cloned plants. When the gas in the culture bottle needs to be replaced, the ventilation box is pressed downwards and then lifted upwards. The whole device can conveniently realize the culture and the character observation of cloned plant organs.
Drawings
FIG. 1 is a schematic front view of the present invention;
FIG. 2 is a three-dimensional schematic view of the upper cover;
FIG. 3 is a top view of the upper cover;
FIG. 4 is a cross-sectional view of the upper cover;
FIG. 5 is a schematic bottom view of the illumination mechanism;
FIG. 6 is a schematic view, partially in section, of an illumination mechanism;
FIG. 7 is a schematic cross-sectional view of a bracket;
FIG. 8 is one of three-dimensional schematic views of a ventilation mechanism;
FIG. 9 is a second three-dimensional schematic of a ventilation mechanism;
FIG. 10 is a schematic cross-sectional view of the ventilation box;
FIG. 11 is a schematic view of the internal structure of the ventilation tube;
fig. 12 is a three-dimensional schematic view of a sealing plug;
FIG. 13 is a schematic view of a dispensing mechanism and a purging mechanism;
FIG. 14 is a three-dimensional schematic of a housing;
FIG. 15 is one of three-dimensional schematic views of a regulator;
FIG. 16 is a second three-dimensional schematic of a regulator;
in the figure: the upper cover, the fixing block, the 111 threaded holes, the 12 connecting pipes, the 13 first inner ring, the 14 first outer ring, the 15 round holes, the 151 slot, the 16 clamping plates, the 17 clamping springs, the 18 clamping holes and the 19 lower communication holes are arranged on the upper cover, the 11 fixing block, the 111 threaded holes, the 12 connecting pipes, the 13 first inner ring, the 14 first outer ring, the 15 round holes, the 151 slot, the 16 clamping plates and the 17 clamping springs;
2 supporting rods, 21 soft rods;
3 lamp box, 31 bracket, 311 mounting hole, 32 vertical axis, 321 top plate, 322 inner rod, 33 optical filter, 34 connecting rod, 35 rotating plate, 351 outer rod, 36 tensioning spring;
4 lower plate, 41 upper plate, 411 upper avoidance hole, 42 telescopic tube, 43 second inner ring, 44 second outer ring, 45 lower avoidance hole, 46 upper communication hole, 47 air cavity;
5 ventilation pipes, 51 baffle plates, 511 ventilation holes, 52 vertical rods, 53 sealing rings, 54 sealing plugs, 541 guide rods, 55 track seats and 56 sealing springs;
6, culturing the bottle;
7 filters, 71 cushion blocks, 72 filter screens, 73 plugs, 74 knobs, 75 transition pipes;
8 outer shell, 80 first through hole, 801 second through hole, 802 third through hole, 803 shaft hole, 81 first conduit, 82 second conduit, 83 mixing tube, 831 snap ring, 84 culture solution barrel, 85 regulator, 851 first inlet, 852 second inlet, 853 outlet, 86 fixed shaft, 87 handle, 88 pointer, 89 mounting ring;
9 measuring tape.
Detailed Description
As shown in fig. 1 to 16, the present invention mainly includes an upper cover 1, a support rod 2, an illumination mechanism, a ventilation mechanism, a culture flask 6, a purification mechanism, a dispensing mechanism, and a measuring tape, and is described in detail below with reference to the accompanying drawings.
As shown in fig. 2 to 4, the upper cover 1 has a cylindrical hollow structure with an open bottom, a fixing block 11 and a connection pipe 12 are fixed to a circular outer wall of the upper cover, and a screw hole 111 is provided on an upper surface of the fixing block. The upper surface of the upper cover is provided with a circular ring-shaped first inner ring 13 and a circular ring-shaped first outer ring 14 which are arranged inside and outside, the center of the upper cover is provided with a round hole 15, the inner wall of the round hole is provided with a pair of slots 151, a semicircular clamping plate 16 is movably arranged in each slot, a clamping spring 17 is arranged between the clamping plate and the inner wall of each slot, the two clamping plates are contacted and enclosed into a circular clamping hole 18 under the action of the clamping spring, and a lower communication hole 19 is arranged between the first inner ring and the first outer ring. In use, the stem of the cloned plant is placed in the holding well.
As shown in fig. 1, a support rod 2 is mounted on a threaded hole of the fixing block in a threaded manner, a soft rod 21 is fixed on the top of the support rod, the soft rod is a gooseneck, and an illumination mechanism is fixed on the soft rod.
As shown in fig. 5 and 6, the lighting mechanism comprises a lamp box 3 fixed on the upper part of the soft rod, a lighting lamp arranged in the lamp box, a bracket 31 fixed on the outer wall of the lamp box, a rotating plate 35 rotatably arranged in the bracket, an outer rod 351 fixed on the bottom of the rotating plate, a top plate 321 arranged below the rotating plate, an inner rod 322 fixed on the top plate, a tension spring 36 arranged between the top plate and the rotating plate, a vertical shaft 32 arranged on the bottom of the top plate, a plurality of connecting rods 34 fixed on the outer wall of the vertical shaft, and a light filter 33 fixed on the connecting rods, wherein the upper part of the inner rod is arranged in the inner cavity of the outer rod and is in sliding connection with the inner rod and the outer rod. As shown in fig. 7, a mounting hole 311 is provided in the bracket, the mounting hole includes three circular holes sequentially provided from top to bottom, the diameter of the middle circular hole is smaller than that of the upper and lower circular holes, and the rotating plate is rotatably mounted in the upper circular hole. The light transmittance of each optical filter is different, and the corresponding optical filter is selected according to the required illumination intensity; when the light box is operated, the vertical shaft is pulled downwards firstly, then the vertical shaft is rotated, the light filter corresponding to the light transmittance is rotated to the position right below the light box, then the vertical shaft is loosened, at the moment, the light filter is contacted with the bottom of the light box under the action of the tensioning spring, and the top plate is arranged in the circular hole below.
The top of upper cover is equipped with the ventilation box, and as shown in fig. 9 to 11, the ventilation box includes upper plate 41, flexible pipe 42 and the hypoplastron 4 that top-down set gradually, and flexible pipe's upper end links firmly and seals well with the upper plate, and flexible pipe's lower extreme links firmly and seals well with the hypoplastron. As shown in fig. 10, the telescopic tube comprises an inner layer and an outer layer, an air cavity 47 is enclosed between the inner layer and the outer layer of the telescopic tube, an upper avoiding hole 411 is arranged at the center of the upper plate, a lower avoiding hole 45 is arranged at the center of the lower plate, and the upper and lower avoiding holes are matched with the clamping holes on the upper cover to clamp the stem of the cloned plant. A second inner ring 43 and a second outer ring 44 are provided at the bottom of the lower plate, the annular region between the second inner ring and the second outer ring corresponds to the air chamber, and an upper communication hole 46 is provided between the second inner ring and the second outer ring, and the upper communication hole communicates with the air chamber. An air exchanging pipe 5 is fixed at the top of the upper cover and is communicated with the air cavity.
During installation, the second outer ring is in threaded connection with the first outer ring, and the second inner ring is in threaded connection with the first inner ring. At this time, the ventilation pipe is communicated with the inner cavity of the upper cover through the air cavity, the upper communication hole and the lower communication hole, and the first inner ring, the second inner ring, the first outer ring and the second outer ring are sequentially arranged from inside to outside.
As shown in fig. 11, a pair of baffles 51 are provided in the inner cavity of the ventilation pipe, and the baffles are screwed with the ventilation pipe and are well sealed. The baffle plates are provided with a plurality of vent holes 511 uniformly arranged along the same circumference, and a circular sealing ring 53 is fixed on the inner wall of the ventilation pipe between the two baffle plates and is a rubber piece. A sealing plug 54 is arranged on the inner side of the sealing ring and is of a hollow structure so as to reduce weight; as shown in fig. 12, the sealing plug comprises three coaxially arranged cylinders. Guide rods 541 are fixed at the top and the bottom of the sealing plug, a track seat 55 is fixed at the bottom of the upper baffle plate, a track seat 55 is also fixed at the top of the lower baffle plate, the guide rods are in one-to-one correspondence with the track seats, and the guide rods are arranged in the inner cavities of the track seats. A sealing spring 56 is arranged between the sealing plug and the track seat, and the stiffness coefficient and the length of the two sealing springs are the same. Under the effect of the sealing spring, the sealing plug is tightly contacted with the inner wall of the sealing ring, so that the sealing of the ventilation pipe is realized. During installation, the upper surface of the upper baffle plate is coplanar with the upper surface of the ventilation pipe, the lower surface of the lower baffle plate is flush with the lower surface of the ventilation pipe, and the deformation amount of the sealing spring is zero. A vertical rod 52 of a regular hexagonal prism shape is fixed on the top of the upper stop rod so as to facilitate the assembly and disassembly of the upper baffle plate. One end of the sealing spring is fixedly connected with the sealing plug, and the other end of the sealing spring is contacted with the track seat. The ventilation box, the ventilation pipe, the sealing plug, the sealing ring, the sealing spring, the track seat, the guide rod and the baffle plate form a ventilation mechanism.
The upper cover is arranged on the bottle mouth of the culture bottle 6 in a threaded manner, the culture bottle is a transparent glass bottle, when the sealing plug moves upwards or downwards until the sealing plug moves out of the sealing ring, the whole ventilation pipe is communicated up and down, gas outside the ventilation pipe can enter the culture bottle through the air cavity, the upper communication hole and the lower communication hole, and the gas inside the culture bottle can be discharged through the lower communication hole, the upper communication hole, the air cavity and the ventilation pipe.
When the air-exchanging box is pressed downwards, the cavity pressure formed by the culture bottle, the upper cover and the air-exchanging box is increased, so that the sealing plug is pushed to move upwards until the sealing plug is separated from the sealing ring, and at the moment, the air in the culture bottle is discharged through the upper cover, the air-exchanging box and the air-exchanging pipe; when the air-exchanging box is lifted upwards, the cavity pressure formed by the culture bottle, the upper cover and the air-exchanging box is reduced, and then the sealing plug is pushed to move downwards until the sealing plug is separated from the sealing ring, and at the moment, external air enters the culture bottle through the air-exchanging pipe, the air-exchanging box and the upper cover. When the air exchanging device is used, the baffle above the air exchanging device can be rotated to push the sealing plug to move downwards, so that the sealing plug is separated from the sealing ring, the air exchanging pipe is opened at the moment, and the culture bottle can exchange air with the outside automatically.
The connection pipe is provided with a distribution mechanism and a purification mechanism, the distribution mechanism comprises a shell 8, a first conduit 81, a second conduit 82, a mixing pipe 83, a culture solution barrel 84 and a regulator 85, the shell is of a cylindrical hollow structure, the circular outer wall of the shell is provided with a first through hole 80, a second through hole 801 and a third through hole 802 which are sequentially arranged along the clockwise direction, the lower end of the first conduit is fixed in the first through hole, the left end of the second conduit is fixed in the second through hole, and the upper end of the mixing pipe is fixed in the third through hole, as shown in fig. 14. A clamping ring 831 is arranged on the side surface of the mixing pipe, and the pipe connection between the mixing pipe and the connecting pipe is realized through the clamping ring. A culture liquid tank 84 is fixed to the upper end of the first conduit, and a valve is provided in the first conduit.
As shown in fig. 15 and 16, the regulator 85 is a cylindrical hollow structure, a first inlet 851, a second inlet 852 and an outlet 853 are sequentially arranged on the outer wall of the regulator in a clockwise direction, the outer wall of the regulator is in close contact with the inner wall of the shell, a fixed shaft 86 is arranged on the axis of the regulator, the fixed shaft penetrates through a shaft hole 803 in the center of the shell and then extends out of the shell for a section, a handle 87 is fixed on the fixed shaft arranged outside the shell, and the rotation of the handle can drive the regulator to rotate so as to regulate the communication size between the first inlet and the first conduit and the communication size between the second inlet and the water outlet pipe, and further regulate the proportion of culture solution and tap water entering the regulator; and when the communication port between the first inlet and the first conduit is gradually increased, the communication port between the second inlet and the water outlet pipe is gradually decreased. The mixed culture solution and tap water enter a mixing tube, and proportional scale marks are also arranged on the outer wall of the shell; the proportion scale mark indicates the proportion of the culture solution in the mixed liquid consisting of the culture solution and tap water entering the regulator. As shown in fig. 1, a pointer 88 is fixed on the fixed shaft, and when the pointer indicates a certain proportion of scale marks a, the ratio of the culture solution entering the mixing tube is a%, and the ratio of tap water entering the mixing tube is 100% -a%; the value of A ranges from 0 to 100, inclusive of 0 and 100.
The purifying mechanism comprises a U-shaped filter 7, the left end of the filter is connected with a second conduit 82, and the right end of the second conduit is provided with a mounting ring 89 which is in threaded connection with the filter. A transition pipe 75 is arranged at the right end of the filter, and a mounting ring is also arranged at the bottom of the transition pipe and is in threaded connection with the filter. The inner walls of the two ends of the filter are fixedly provided with cushion blocks 71, the filter screen 72 is placed on the cushion blocks, the bottom of the filter is provided with a slag discharge port, a plug 73 is arranged on the slag discharge port in a threaded manner, and a knob 74 is fixed at the bottom of the plug so as to facilitate the disassembly of the plug; after the plug is removed, impurities in the filter can be discharged. The transition pipe is used for being connected with the water pipe, tap water enters the filter through the transition pipe, then the tap water is filtered under the action of the filter screen, impurities in the tap water are filtered, and the tap water after the impurities are filtered enters the regulator through the second guide pipe. The filter is arranged into a U-shaped structure, so that impurities in tap water can be conveniently precipitated, the buffer effect on the water flow is achieved, tap water can be conveniently and fully contacted with the filter screen, and the purifying effect is guaranteed. Tap water is purified firstly and then enters a distribution mechanism, and the proportion of the culture solution to the tap water is regulated by the distribution mechanism so as to meet the growth requirement of cloned plant organs.
As shown in fig. 1, in order to measure the stem length of the cloned plant in the culture flask, a measuring scale 9 vertically placed is fixed in the culture flask, and the stem length of the cloned plant is measured by the measuring scale.
The application method of the dynamic observation device for growth of the wetland plant clone organ comprises the following steps:
(1) Placing stems of cloned plants in the clamping holes of the upper cover, and installing an air ventilation box at the top of the upper cover;
(2) Adding culture solution into a culture solution barrel, connecting a transition pipe with a tap water pipe, adding tap water into the transition pipe, and adjusting the ratio of the culture solution to tap water entering a culture bottle according to the requirement;
(3) Turning on an illuminating lamp according to the requirement, and adjusting the brightness of the irradiated clone plants;
(4) The ventilation box is pressed as required to allow the flask to exchange gas with the outside.
Claims (3)
1. The dynamic observation device for growth of the wetland plant clone organ is characterized by comprising an upper cover, a culture bottle, a supporting rod, an illumination mechanism, a ventilation mechanism and a distribution mechanism, wherein the upper cover is arranged on the bottle mouth of the culture bottle in a threaded manner, a round hole for placing plant stems is formed in the center of the upper cover, a pair of slots are formed in the inner wall of the round hole, clamping plates are movably arranged in the slots, a clamping spring is arranged between the clamping plates and the inner wall of the slots, and the two clamping plates are contacted and enclose a clamping hole under the action of the clamping spring; a measuring ruler which is vertically placed is arranged in the culture flask, a supporting rod is detachably arranged on the side wall of the upper cover, a soft rod is fixed at the top of the supporting rod, and an illumination mechanism with adjustable illumination intensity is arranged on the soft rod; the illuminating mechanism comprises a lamp box fixed on the soft rod, an illuminating lamp arranged in the lamp box, a bracket fixed on the lamp box, a rotating plate rotatably arranged in the bracket, a top plate arranged below the rotating plate, a tensioning spring arranged between the top plate and the rotating plate, a vertical shaft fixed at the bottom of the top plate, a plurality of optical filters arranged around the vertical shaft, and a connecting rod arranged between the optical filters and the vertical shaft, wherein the optical transmission of each optical filter is different; an outer rod is fixed at the bottom of the rotating plate, an inner rod is fixed at the top of the top plate, and the upper part of the inner rod is arranged in an inner cavity of the outer rod and is in sliding connection with the outer rod;
the top of the upper cover is provided with a ventilation mechanism, the ventilation mechanism comprises a ventilation box and a ventilation pipe, the ventilation box comprises an upper plate, a telescopic pipe and a lower plate which are sequentially arranged from top to bottom, the lower plate is in threaded connection with the top of the upper cover, the lower plate and the top of the upper cover are well sealed, the telescopic pipe comprises two layers which are internally and externally arranged, the telescopic pipe, the upper plate, the telescopic pipe and the lower plate are well sealed, and an air cavity is formed between the inner layer and the outer layer of the telescopic pipe in a surrounding manner; an upper communication hole is formed in the lower plate, a lower communication hole communicated with the upper communication hole is formed in the top of the upper cover, an air exchanging pipe communicated with the air cavity is fixed at the top of the upper plate, a pair of baffles which are arranged up and down are detachably arranged on the inner side of the air exchanging pipe, a sealing ring fixedly connected with the inner wall of the air exchanging pipe is arranged between the two baffles, a sealing plug is arranged between the two baffles, track seats are fixed on the baffles, guide rods are respectively fixed at the top and the bottom of the sealing plug, the guide rods are in sliding connection with the corresponding track seats, sealing springs are arranged between the track seats and the sealing plug, and the sealing plug is in tight contact with the inner wall of the sealing ring under the action of the two sealing springs; an upper avoidance hole is formed in the center of the upper plate, a lower avoidance hole is formed in the center of the lower plate, and the round hole, the upper avoidance hole and the lower avoidance hole cooperate to clamp stems of plants;
the device comprises an upper cover, a connecting pipe, a distributing mechanism, a fixed shaft, a culture solution barrel, a first inlet, a second inlet and an outlet, wherein the connecting pipe is fixed on the outer wall of the upper cover;
still include purifying mechanism, purifying mechanism includes the filter of the U-shaped with second pipe threaded connection, sets up the filter screen at filter both ends inside, is equipped with the row's cinder notch in the bottom of filter, is equipped with the transition pipe in the right-hand member of filter, installs the stopper on row's cinder notch screw thread.
2. The dynamic observation device for growth of plant cloned organs of wetland according to claim 1, wherein a first inner ring and a first outer ring which are arranged inside and outside are arranged at the top of the upper cover, a second inner ring and a second outer ring which are arranged inside and outside are arranged at the bottom of the lower plate, the second outer ring is in threaded connection with the first outer ring, the second inner ring is in threaded connection with the first inner ring, and the first inner ring, the second inner ring, the first outer ring and the second outer ring are sequentially arranged from inside to outside.
3. The method of using the dynamic observation device for growth of plant clone organs of the wetland according to any one of claims 1 to 2, characterized in that it comprises the following steps:
(1) Placing stems of cloned plants in the clamping holes of the upper cover, and installing an air ventilation box at the top of the upper cover;
(2) Adding culture solution into a culture solution barrel, connecting a transition pipe with a tap water pipe, adding tap water into the transition pipe, and adjusting the ratio of the culture solution to tap water entering a culture bottle according to the requirement;
(3) Turning on an illuminating lamp according to the requirement, and adjusting the brightness of the irradiated clone plants;
(4) The ventilation box is pressed as required to allow the flask to exchange gas with the outside.
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Citations (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3022703U (en) * | 1995-09-14 | 1996-04-02 | 清奇 謝 | Culture bottle |
| CN201167508Y (en) * | 2008-03-14 | 2008-12-24 | 莫家贤 | Plant cultivation pot |
| CN203860185U (en) * | 2014-05-14 | 2014-10-08 | 杭州电子科技大学 | Novel flowerpot structure |
| CN204443322U (en) * | 2015-01-27 | 2015-07-08 | 蒋桂欣 | Multifunction plant cultivating device |
| CN204482473U (en) * | 2014-11-05 | 2015-07-22 | 东莞骅国电子有限公司 | Plant incubator |
| CN205357344U (en) * | 2016-02-02 | 2016-07-06 | 白艳萍 | Three -dimensional greening case |
| CN105972743A (en) * | 2016-06-20 | 2016-09-28 | 翁正祥 | Mist culture type air purifier |
| CN106035036A (en) * | 2016-05-30 | 2016-10-26 | 吉林省农业科学院 | Water culturing device suitable for whole-growing-period testing of soybean |
| CN106332759A (en) * | 2016-08-26 | 2017-01-18 | 魏满妹 | Implementation method for culturing plants in culture flask |
| CN205946812U (en) * | 2016-05-23 | 2017-02-15 | 青海省畜牧兽医科学院 | Plant culture dish |
| CN106386453A (en) * | 2016-11-17 | 2017-02-15 | 宁夏荣光电节能科技实业有限公司 | Plant planting and illuminating integrated device and control system thereof |
| CN106386451A (en) * | 2016-11-02 | 2017-02-15 | 广西壮族自治区农业科学院经济作物研究所 | Aeroponic cultivation device for observing growth development of plant |
| CN106434314A (en) * | 2016-11-02 | 2017-02-22 | 广东石油化工学院 | Culture bottle air change device |
| CN206005426U (en) * | 2016-08-26 | 2017-03-15 | 程依清 | A kind of plant culture bottle |
| CN206212884U (en) * | 2016-07-28 | 2017-06-06 | 北京天通泰农业科技发展有限公司 | A kind of hothouse plants automatic liquid supply culture apparatus |
| CN206760183U (en) * | 2017-04-19 | 2017-12-19 | 福建东进农业科技有限公司 | A kind of succulent planting unit |
| CN107548980A (en) * | 2017-08-18 | 2018-01-09 | 陈伟 | A kind of method of full-automatic continuous-flow type plant culture |
| CN108157157A (en) * | 2018-02-09 | 2018-06-15 | 唐麒华 | Plant cultivation box and the growth device for plant using the plant cultivation box |
| CN207610039U (en) * | 2017-11-07 | 2018-07-13 | 东莞市怡佳照明科技有限公司 | A kind of novel plant oxygen life lamp |
| CN108419662A (en) * | 2018-05-07 | 2018-08-21 | 佛山科学技术学院 | A kind of aerosol training device may act as light source |
| CN209201741U (en) * | 2018-10-24 | 2019-08-06 | 杭州师范大学 | Wetland plant clone organ Growth trends observation device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10072880B2 (en) * | 2015-09-14 | 2018-09-11 | Herbert Newsam | Self-contained plant cloning system and method |
-
2018
- 2018-10-24 CN CN201811242168.8A patent/CN109392684B/en active Active
Patent Citations (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3022703U (en) * | 1995-09-14 | 1996-04-02 | 清奇 謝 | Culture bottle |
| CN201167508Y (en) * | 2008-03-14 | 2008-12-24 | 莫家贤 | Plant cultivation pot |
| CN203860185U (en) * | 2014-05-14 | 2014-10-08 | 杭州电子科技大学 | Novel flowerpot structure |
| CN204482473U (en) * | 2014-11-05 | 2015-07-22 | 东莞骅国电子有限公司 | Plant incubator |
| CN204443322U (en) * | 2015-01-27 | 2015-07-08 | 蒋桂欣 | Multifunction plant cultivating device |
| CN205357344U (en) * | 2016-02-02 | 2016-07-06 | 白艳萍 | Three -dimensional greening case |
| CN205946812U (en) * | 2016-05-23 | 2017-02-15 | 青海省畜牧兽医科学院 | Plant culture dish |
| CN106035036A (en) * | 2016-05-30 | 2016-10-26 | 吉林省农业科学院 | Water culturing device suitable for whole-growing-period testing of soybean |
| CN105972743A (en) * | 2016-06-20 | 2016-09-28 | 翁正祥 | Mist culture type air purifier |
| CN206212884U (en) * | 2016-07-28 | 2017-06-06 | 北京天通泰农业科技发展有限公司 | A kind of hothouse plants automatic liquid supply culture apparatus |
| CN106332759A (en) * | 2016-08-26 | 2017-01-18 | 魏满妹 | Implementation method for culturing plants in culture flask |
| CN206005426U (en) * | 2016-08-26 | 2017-03-15 | 程依清 | A kind of plant culture bottle |
| CN106386451A (en) * | 2016-11-02 | 2017-02-15 | 广西壮族自治区农业科学院经济作物研究所 | Aeroponic cultivation device for observing growth development of plant |
| CN106434314A (en) * | 2016-11-02 | 2017-02-22 | 广东石油化工学院 | Culture bottle air change device |
| CN106386453A (en) * | 2016-11-17 | 2017-02-15 | 宁夏荣光电节能科技实业有限公司 | Plant planting and illuminating integrated device and control system thereof |
| CN206760183U (en) * | 2017-04-19 | 2017-12-19 | 福建东进农业科技有限公司 | A kind of succulent planting unit |
| CN107548980A (en) * | 2017-08-18 | 2018-01-09 | 陈伟 | A kind of method of full-automatic continuous-flow type plant culture |
| CN207610039U (en) * | 2017-11-07 | 2018-07-13 | 东莞市怡佳照明科技有限公司 | A kind of novel plant oxygen life lamp |
| CN108157157A (en) * | 2018-02-09 | 2018-06-15 | 唐麒华 | Plant cultivation box and the growth device for plant using the plant cultivation box |
| CN108419662A (en) * | 2018-05-07 | 2018-08-21 | 佛山科学技术学院 | A kind of aerosol training device may act as light source |
| CN209201741U (en) * | 2018-10-24 | 2019-08-06 | 杭州师范大学 | Wetland plant clone organ Growth trends observation device |
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