JP3022703U - Culture bottle - Google Patents

Abstract

(57) Abstract: A culture bottle for aseptic culture. SOLUTION: One bottle body having a bottle mouth at the bottom end and a substantially cylindrical body having an outer diameter smaller than the inner diameter of the bottle mouth are provided to support a culture medium, seeds, etc. and enter the bottle body. It is used to make the bottle body contact with the bottom end of the bottle mouth and has a flange around the bottom end which has a hole through which the inside and the outside of the bottle body pass and the inside of the bottle body is screwed to the bottle body. It is screwed to tightly fit the object board in the bottle body, and at least one airway is opened at a predetermined position, and a combination of the airway and a filter cotton sandwiched between the object board The inside of the bottle body is communicated with the outside air by means of the above, and one lid is provided for filtering impurities such as ash dust to prevent contamination inside the bottle body.

Description

[Detailed description of the device]

[0001]

[Technical field to which the device belongs]

 The present invention relates to a kind of culture bottle, and more particularly, to a culture bottle which is convenient for cleaning and disinfecting operations, can be collected and reused, has a low manufacturing cost, and has an increased seedling growth space during aseptic breeding.

[0002]

[Prior art]

 In the process of cultivating seedlings of general plants, varieties and, if necessary, sterile breeding or bacterial breeding are carried out.For the former, by separating from the outside world in the process of seed germination and seedling production, bacteria, Prevents infection by fungi, etc., and most of them are transplanted in the natural environment after forming seedlings in culture bottles. Most of the currently used aseptic breeding uses an Erlenmeyer flask as a culture bottle, and the culture medium rich in nutrients is first placed in the flask, and then the flask is heated, pressurized or chemically treated. After disinfection and sterilization according to the method, the tissue culture tissue of the seed or plant is placed in an aseptic operating environment, and the bottle mouth is tightly closed with a non-translucent wooden stopper (or plastic stopper). A hole is formed in the central part of the tube in the axial direction, and a cotton strip is placed in the hole to prevent the air in the flask from communicating with the outside world through the cotton strip and directly contacting it. It prevents the flask from bursting due to the thermal expansion of the internal air after it is irradiated with light, and by tying it by wrapping it with a plastic film (sheet) in addition to providing a wooden stopper at the bottle mouth. , Outside Of dust and bacteria, so that pollution in the by Ri flask to the entry of microorganisms such as fungi are not formed. However, since such a conventional culture bottle uses a general Erlenmeyer flask as it is, the bottle mouth is too small, so that the pretreatment disinfection process and the work of placing the culture medium and seeds proceed. Difficulty in removing seeds for transplantation after the seeds have germinated and grown, and the flascoes are prone to rupture, which often makes them unusable, thus forming a waste of resources. Furthermore, blocking the bottle mouth with a tree plug hinders relatively partial illumination, and the growth of the plant has a tropism, which has a more or less negative effect on the growth of seedlings. It became a thing. Also, the wooden stopper or plastic stopper is supported by the frictional force between the inner wall of the bottle mouth and the stopper so as not to come off from the bottle mouth, but such a rough and uncertain old method is likely to cause loosening. Therefore, external bacteria and fungi enter the flask to form contamination, which increases the failure rate of culture. Therefore, there has been a demand for a culture bottle that can be used repeatedly and is convenient to use.

[0003]

[Problems to be solved by the device]

 The main purpose of the present invention is to provide a single culture bottle that can be put in and taken out of seedlings and seeds easily, can be easily washed and disinfected, and can be used repeatedly.

[0004] Another object of the present invention is to provide a kind of culture bottle which does not provide a stopper on the upper end of the bottle and therefore does not block the illumination of the seedlings and thus can promote its growth sufficiently. Is.

A further object of the present invention is to combine a wide-mouthed bottle mouth with one lid so that the seedlings can be easily taken out, and aseptic planting work in the bottle can be easily progressed. The purpose of the present invention is to provide a single culture bottle that can perform disinfection work more easily than conventional ones, improve work efficiency, and reduce the cost and the failure rate of culture.

[0006]

[Means for Solving the Problems]

 The culture bottle of the present invention has a bottom end which has a large opening area for facilitating the putting and taking out of an article into the inside and has one bottle mouth provided with a screw thread on the outer periphery. A bottle-shaped body with a flange-shaped shield that prevents dust and impurities from entering the inside of the screw thread, and a substantially cylindrical body, the outer diameter of which is smaller than the inner diameter of the bottle mouth, It is used to support culture medium, plants, seeds, fungi, etc. to enter into the bottle body, and one flange is provided around the bottom end, and the outer diameter of the flange is larger than the inner diameter of the bottle mouth. A container for use in tightly sealing the bottle mouth, and at least one pore for communicating the inside and the outside of the bottle body penetrating within the inside of the bottle mouth of the flange; A screw thread that fits with the screw thread of the bottle mouth is provided on the inner circumference. By fitting the piles, the above-mentioned object board is tightly fitted in the bottle body, and at least one airway is opened at a predetermined position, and the filtration is sandwiched between the airway and the object board. It is equipped with a lid that allows the inside of the bottle to communicate with the outside air when combined with cotton and filters impurities such as ash dust to prevent contamination inside the bottle.

Further, the bottle body of the present invention is characterized in that the upper end surface of the bottle body is an arcuate surface which is recessed toward the inside of the bottle body.

[0008]

[Embodiment of device]

 The culture bottle of the present invention has one bottle body, one container, and one lid. Among them, the bottom side of the bottle body is opened to form one bottle mouth, and the bottle mouth is assumed to have a considerable opening area so that articles can be easily placed in the bottle body and taken out from the bottle body. A screw thread is formed on the outer wall around the bottle mouth.

The above-mentioned object board is a substantially cylindrical body, the outer diameter of which is smaller than the inner diameter of the mouth of the bottle so that it can support culture medium, plants, seeds, fungi, etc. and enter the bottle. Is. A flange is provided around the bottom end of the container plate, and the outer diameter of the flange is larger than the inner diameter of the bottle mouth so as to be tightly sealed in the bottle mouth.

One pore penetrates the area inside the bottle mouth of the flange, so that the inside of the bottle and the outside communicate with each other.

A screw thread to be fitted to the screw thread of the bottle mouth is provided on the inner circumference of the lid, and the object board is tightly fitted in the bottle body by fitting the both screw threads. At least one airway is opened at a predetermined position of the lid, and the inside of the bottle body and the outside air communicate with each other by a combination of the airway and a filter cotton sandwiched between the lid and the object board. , And dust and other impurities are filtered to prevent contamination inside the bottle.

The air circulation system of the culture bottle goes to the lower opening, and the flange of the plate further strengthens the tightness of the bottle body and the lid, and the inside of the bottle is contaminated by dust falling outside the bottle. The infection rate of non-bacterial seedlings will be drastically reduced, and the possibility that the inside of the bottle will be thoroughly contaminated will be eliminated.

[0013]

【Example】

 The culture bottle of the present invention includes one bottle body 10, one container 20 and one lid 30, in which the bottle body 10 is made of a translucent material, and the upper surface thereof is It has a concave arc surface. One bottle mouth 12 is opened to the outside at the bottom of the bottle body 10. The bottle mouth 12 has an opening area convenient for putting an article in the bottle body 10 or taking out an article from the bottle body 10. And a thread 14 is provided on the outer periphery thereof, and a flange-shaped shield 16 is formed on the upper end of the thread 14. Further, the above-mentioned object board 20 is made into one cylindrical body, and is used for supporting the culture medium, plant seeds, fungi, etc. The outer diameter of the cylindrical object of the object board 20 is the above-mentioned bottle mouth 12 Since it is smaller than the inner diameter of the bottle, it can enter the inner space of the bottle body 10 from the bottle mouth 12. One flange 22 is provided on the outer periphery of the bottom end of the object board 20, the outer diameter of the flange 22 is provided larger than the inner diameter of the bottle mouth 12, and the flange 22 is made of a material having appropriate elasticity. The bottle mouth 12 can be closed by bringing the flange 22 into contact with the bottom end surface of the bottle mouth 12. Two pores 222 are formed at a predetermined position within the bottle mouth 12 of the flange 22, so that the air inside the bottle body 10 communicates with the outside world, whereby the air inside the bottle body 10 thermally expands. The rupture of the bottle body 10 due to the cold shrinkage is prevented, and the ash dust in the external environment is prevented from contaminating the sterile space inside the bottle body 10 due to the convection of air. Further, one screw thread 31 to be fitted with the screw thread 14 of the bottle mouth 12 of the bottle body 10 is provided on the inner circumference of the lid 30, and the lid 30 is screwed onto the bottom of the bottle body 10 to be supported. It is used to seal the board 20 in the bottle 10. An airway 32 is formed in the center of the lid 30 at a predetermined position, and a filter cotton 40 is sandwiched between the airway 32 of the lid 30 and the object board 20. The filter cotton 40 is made of a considerably dense material and filters the air entering from the airway 32 to remove impurities such as ash dust in the gas and prevent the inside of the bottle body 10 from being contaminated.

As shown in FIG. 2 and FIG. 3, when the culture bottle of the present invention is actually used, the bottle body 10, the container 20 and the lid 30 are pre-disinfected. Detoxification is thorough and easy to perform, improving culture quality and increasing success rate. After disinfection, tissue samples of seeds and plants, and fungi are cultivated using the culture medium on the substrate 20. At this time, since the inlet is not as narrow as in conventional culture bottles, it is easy to adjust the amount of culture medium, seeds, etc., and the work can be performed easily without causing contamination by touching the bottle body. , Saves time. After the placement of the culture medium and seeds is completed, the parts are simply assembled in order in a sterile environment, and the planting is completed. Here, since the material of the flange 22 has elasticity, the lid 30 and the bottle body 10 are tightly coupled. Further, if all the culture bottles are placed on the mesh shelf in order, since the airway 34 is provided at the bottom, the flow of air is not obstructed, and at the same time, the shielding part 16 shields impurities such as dust from the lid 30. It does not enter from between the bottle bodies 10 and contaminate the inside. Due to this design that vents from below, the air flows appropriately inside and outside the bottle, it is difficult to create stains, and there is no need for the conventional procedure of covering with a plastic film or sheet and binding, and the work process And save time and money. When the seedlings in the bottle are taken out after they have grown to a certain extent, simply swiveling the lid 30 to remove the seedling board 20 with the plant can be taken out together, and the bottle body 10 must be broken. In addition, the bottle body 10, the object board 20 and the like can be easily cleaned and disinfected, and then reused. Therefore, the utilization rate of resources is increased and the cost is reduced. Further, since the upper end of the bottle body 10 is not provided with a conventional light-tight wooden plug or the like and the upper surface of the bottle body 10 is inwardly concave, the influence of blocking light irradiation is affected. In addition, when light rays are applied, the light rays cause a phenomenon of scattering in the bottle body 10 due to the principle of refraction of light in the lens, and the light rays are evenly distributed in the inner space of the bottle body 10. Each part of the plant can receive a uniform light beam, and the growth mode is good. Therefore, even when cultivating a large number of seedlings at one time, some seedlings grow faster due to insufficient irradiation of light, It prevents the irradiation of other strains from being exposed to light and prevents the growth from slowing down, thus ensuring uniform culture quality.

[0015]

EFFECTS OF THE INVENTION The culture bottle of the present invention makes it possible to increase the growth space of seedlings by taking advantage of the new bottle structure and spatial morphology, and to easily take out the seedlings after growth for transplantation. The body can be easily cleaned or does not require cleaning, or can be reused in duplicate after disinfection to achieve the effect of resource utilization.

The present invention also does not block the illumination of the seedlings by virtue of the fact that there is no plugging object at the upper end of the bottle, and therefore it can fully promote its growth.

Furthermore, the present invention combines a wide-mouthed bottle mouth shape with one lid so that the seedlings can be easily taken out, and aseptic transplantation work in the bottle can be easily progressed, and sterilization work for sealing and opening the bottle is performed. Can be performed more easily than conventional ones, work efficiency can be improved, and cost and culture failure rate can be reduced.

[Brief description of drawings]

FIG. 1 is a perspective view of a conventional sterile culture bottle.

FIG. 2 is an exploded perspective view of the present invention.

FIG. 3 is a sectional view showing a usage state of the present invention.

[Explanation of symbols]

19 ... Bottle body 20 ... Object board 30 ... Lid 1
2 ... Bottle mouth 14 ... Thread 16 ... Shield 22 ... Flange 222 ... Pore 31 ... Thread 32 ... Airway 40 ... Filter cotton

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor, Yuan Lei, Taiwan, Nantou, Nobuyoshi Town, Zhiqiang Town, Yanghe Town, No. 69, (72) Inventor, Yi Rong Jing, Taichung, Taichung, Taiwan, No. 35, 46, No.

Claims (2)

[Scope of utility model registration request] 1. A bottle mouth, which has a bottom end having a large opening area for facilitating the putting and taking out of an article into the inside by opening, and has a screw thread provided on the outer periphery, is formed on the screw thread. A bottle-shaped body having a flange-shaped shielding part for preventing dust and impurities from entering the inside and a substantially cylindrical body, the outer diameter of which is smaller than the inner diameter of the bottle mouth, and the culture medium, It is used to support plants, seeds, fungi, etc. to enter into the bottle body, and one flange is provided around the bottom end, and the outer diameter of the flange is larger than the inner diameter of the bottle mouth. It is used for tightly sealing the mouth, and at least one pore for communicating the inside and the outside of the bottle body is penetrated in the range of the inside of the bottle mouth of the flange.
One object board and a screw thread to be fitted with the screw thread of the bottle mouth are provided on the inner circumference, and by fitting both threads, the object board is tightly fitted in the bottle body. At least one airway is pierced at the position of, and the inside of the bottle body is communicated with the outside air by a combination of the airway and a filter cotton sandwiched between the object board and impurities such as ash dust are filtered. A culture bottle with a lid to prevent the inside of the bottle from being contaminated. 2. The culture bottle according to claim 1, wherein an upper end surface of the bottle is an arcuate surface that is recessed toward the inside of the bottle.