JP3035384U - Incubator - Google Patents

Abstract

(57) Abstract: A plant tissue culture that can be advantageously used when performing various forms of plant tissue culture using a liquid culture medium by changing the medium in the same container to reduce the chance of contamination. Incubator V for. SOLUTION: A transparent container (CO) provided with a removable cap (Ca) and a membrane (M) communicating with the lower part thereof.
A side tube (ST) provided with a cap (Cp) having the above and a float member (F) provided with a porous culture tissue support sheet (PS) are the main constituent elements, and a vessel (V) is provided by the cap (Ca). Limit the inflow of pollutants into the inside of the side pipe (S
Using T), the culture medium is monitored, the culture medium is maintained at a predetermined level, and the inflow and outflow of the culture medium are adjusted. Tissue culture is performed without contaminating the tissue.

Description

[Detailed description of the invention]

[0001]

[Technical field to which the invention belongs]

 INDUSTRIAL APPLICABILITY The present invention relates to an incubator for culturing plant tissue, and is particularly useful for in vitro restoration of small plants and large-scale growth.

[0002]

[Prior art]

 It is now well known to use solid and liquid culture media for in-vitro regeneration and large-scale growth of the above plantlets. However, the use of liquid culture media in the conventional process of plantlet regeneration in glassware facilitates further oxygen and nutrient uptake by cells / tissues which enhances production levels. , Have been found to be even more effective. Incubators known in the art are usually considered rather suitable for solid culture media, which provides improved usage of liquid culture media, which is inherent to their structural young. This is because of restrictions. When carrying out the various stages of culture, it is important for plant tissue culture in liquid culture medium that the plant tissue in culture is not contaminated either by the atmosphere or by improper handling of the tissue. In addition, during use of the liquid culture medium, it is necessary to monitor the culture medium regularly to ensure the proper amount of liquid medium to facilitate proper uptake of the medium for its culture by the tissue. Is. Furthermore, in order to carry out various stages of tissue culture, it is necessary to change the liquid culture medium from one formulation to another during the culture process. When changing the liquid culture medium, it is necessary not to contaminate the tissue. In the art, conventional incubators currently in use are unsuitable for plant tissue culture using liquid culture media.

[0003]

[Problems to be solved by the device]

 An object of the present invention is to advantageously utilize a variety of plant tissue cultures using a liquid culture medium by changing the medium in the same vessel to reduce the chance of contamination. To bring the incubator.

[0004]

[Means for Solving the Problems]

 According to the invention, an incubator is provided with a transparent container with a detachably mounted cap, and with a detachably mounted cap, the lower part of said container via at least one passage. At least one set of side tubes in fluid communication with, wherein the side tubes are arranged at an angle to the wall of the container, the side tubes are arranged in the container and are of a porous tissue-supporting type for culturing. An incubator is obtained which comprises at least one set of float members with a support sheet.

The incubator of the present invention enables tissue culture without contaminating the tissue. A removable cap on the vessel limits the inflow of contaminants into the vessel during culture. In addition, the side tube may be used to change the necessary monitoring of the liquid culture medium and / or to maintain a certain predetermined level of liquid medium in the vessel, and / or to allow drainage into / out of the vessel as needed and / or needed. It prepares for the outing. The requirement to transfer tissue from one vessel to the other in order to present it to the culture stage is also a requirement in the same vessel by introducing an appropriate liquid medium into the vessel via the side tube at each stage of culture. It is carried out in. The side tube is also provided with a removable cap at its open end to limit the inflow of any contaminants through the tube into the vessel.

[0006]

[Embodiment of the invention]

 With reference to FIG. 1, the incubator V here includes a container CO which may be cylindrical with a screw cap Ca or of any other shape. The container CO is provided with a side pipe ST which is arranged so as to be inclined with respect to the side wall WS of the container CO. The side tube ST has a cap Cp removably held therein. As shown in FIG. 3, the cap Cp has a membrane made of any suitable resilient or pierceable material to allow insertion and withdrawal of a syringe needle. The side tube ST communicates with the inside of the container CO via the passages 01 and 02 provided in the side wall WS. The passages 1 and 2 are arranged in a spaced relation to each other, so that the passage 01 allows the introduction, addition or withdrawal of the culture medium into the container CO without removal of the cap Ca. The passage 02 is arranged at a certain height above the passage 01 and weakens the vacuum in the side tube ST. The container CO is shaped like an arrow and is equipped with a float member F which may be of any other shape. The shapes of the container CO and the float member F are not important. The float F has a porous sheet PS. According to one embodiment, as shown in Fig. 2, a radially inwardly extending flange FL is provided on the float F, which comprises the porous sheet PS retained therein. Alternatively, as shown in FIG. 3, the flange FL supports the porous sheet PS. The tissue is placed on the porous sheet PS, which at the same time allows the tissue to absorb nutrients from the liquid medium. Thus, the tissue for culturing is placed on the porous sheet PS, and then the float F is introduced through the open end of the container CO. The cap Ca then closes the container CO in order to bring the atmosphere free of pollutants to the tissue. Attachment of the cap Ca to the container C O can be done either by continuous or discontinuous threads, or by some press-fit connection of the cap Ca with the container CO. The liquid medium required for tissue culture is fed into the container CO through the side tube ST and the passage 01. As soon as the liquid medium occupies the container CO below the float F, the float F rises and floats above the liquid medium. It is lower than the height of the second passage 02 because the level of the liquid medium is maintained up to the maximum upper limit. The transparency of the container CO and the side tube ST further facilitates constant monitoring of the level of the liquid medium in the container CO. During tissue culture, the liquid medium is wicked by the tissue and this type of wicking of the liquid medium causes the liquid level to begin to drop. The above-mentioned drop of the level of liquid medium in the container CO is noted and is maintained by the introduction of fresh liquid medium via the side tube ST. Similarly, if it is important to change the liquid medium when carrying out the various stages of the culture process, the side tube ST is effective for draining and / or introducing the liquid medium required for plant tissue growth. Can be used for. The side tube ST is provided with a cap Cp that is detachably attached. The cap Cp according to the preferred embodiment has a membrane M made of rubber or any pierceable material for facilitating the discharge of the introduction of the culture medium into the container CO via the side tube ST. . The detachably mounted cap Cp has a threaded connection to the side tube or can be press fit onto the upper end of the side tube. Once the tissue is supported on the sheet PS, the needle of the syringe can be used to introduce, supplement, or withdraw the culture medium in order to maintain a contaminant-free atmosphere in the container CO. Be seen.

As shown in FIGS. 2 and 3, the float F includes an inner wall F1 that is spaced from an outer wall F2 to impart the required strength to the float F.

The threaded press-fit connection of the caps Ca and Cp can be provided with continuous and / or discontinuous threads to prevent the entry of contaminants into the incubator V. Desirably, as shown in FIG. 3, the cap Ca is provided with an inclined bottom surface SL extending radially inward and downward. The tilted ramp cap prevents contamination caused by blocked condensation droplets held along the cap wall joint and / or their drip along the wall of the container.

The incubator of the present invention and its side tube shown in the cylindrical shape described above in connection with the accompanying drawings of the preferred embodiment have any other shape, namely triangular, circular, square, long. Obviously, it may be square, elliptical and polygonal. Further, the shapes of the float member and the porous support structure PS can be changed according to the shape of the container. Containers and side tubes are selected from glass, polymeric materials and stainless steel, which can be placed in pressure cookers or sterilized materials, transparent materials, translucent materials, non-corrosive metal / non-metal composite It can be made from the specified materials. The float can have solid or hollow sidewalls and can be made from non-corrosive metal / non-metal composite materials such as polymeric materials or stainless steel. The central support structure can be porous / perforated / woven and made from non-corrosive metal / non-metal composite materials such as glass wool, polymeric materials and stainless steel. The support structure can be integral with the float matrix and is adapted to be supported by a radially inwardly extending flange and / or the like of the float provided in the float member. But it's okay.

It should be determined from the viewpoint that it is possible to change the incubator of the present invention described above, and the scope of the present invention can change the disclosure of the present invention. In this regard, the incubator of the present invention may have multiple side tubes instead of the one side wall described in the preferred embodiment above.

[Brief description of the drawings]

FIG. 1 is a schematic view of an incubator according to the present invention showing a container containing a float member having a porous sheet attached with a cap and supported inside the float.

FIG. 2 is a sectional view of the float member showing installation of a flange supporting the porous sheet near the lower end of the float member.

FIG. 3 is a further cross-sectional view of the complete incubator taken along line AA ′ of FIG. 1 with a cap attached.

[Explanation of symbols]

 V Incubator CO Container Ca Screw cap Cp Cap 01 First opening 02 Second opening M Membrane WS Side wall PS Porous sheet ST Side tube SL Inclined bottom F Float member F1 Inner wall F2 Outer wall FL Flange

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[Procedure amendment]

[Submission date] September 19, 1996

[Procedure 3]

[Document name to be amended] Statement

[Correction target item name] Claims for utility model registration

[Correction method] Change

[Correction contents]

[Utility model registration claims]

Front page continued (73) Utility model holder 596067618 Vinod Kumar Sefgar India Calcutta, Marshall Haus 818, Netaji Subhahas Road 33/1 (72) Inventor Bimal Chandra Villatta Charya India Karagupa, IT Campus, BF 1 / 12 (72) Inventor Pulsa Sarasi Byattacharya India Karagupa, IT Campus BF 4/3 (72) Creator Sat Yahali India Caragupa, IT Campus, 1-Br-6

Claims (10)

[Utility model registration claims] 1. An incubator, a transparent container with a removably attachable cap, having a removably attachable cap, and being in fluid communication with a lower portion of said container via at least one passageway. An incubator comprising at least one set of side tubes and a porous tissue-supporting support sheet for culturing. 2. The incubator according to claim 1, wherein
The side wall of the container comprises a first opening and a second opening spaced at a height above the first opening,
An incubator in which both of the openings are in fluid communication with the side tube. 3. The incubator according to claim 1,
An incubator in which the float member comprises a porous support sheet and a flange extending radially inward. 4. The incubator according to claim 3,
An incubator in which the support sheet is supported on the flange. 5. The incubator according to claim 3,
An incubator in which the support sheet is fixed to the flange. 6. The incubator according to claim 1, wherein
An incubator in which each of the inner wall and the outer wall is included in the float so that the inner wall is separated from the outer wall. 7. The incubator according to claim 1,
An incubator in which the cap of the side tube is provided with a membrane. 8. The incubator according to claim 1,
An incubator in which the cap of the container has an inwardly descending surface. 9. An incubator as claimed in any one of claims 1 to 8 essentially as described above. 10. The method according to any one of claims 1 to 8.
In the incubator described in paragraph 1, essentially any one of the drawings
Incubator as shown in Figure 1.