CN1138095A - Present invention relates to culture vessel for plattissue culture and is particularly useful for in vitro restoration and mass propagation of plantlets - Google Patents
Present invention relates to culture vessel for plattissue culture and is particularly useful for in vitro restoration and mass propagation of plantlets Download PDFInfo
- Publication number
- CN1138095A CN1138095A CN96107443A CN96107443A CN1138095A CN 1138095 A CN1138095 A CN 1138095A CN 96107443 A CN96107443 A CN 96107443A CN 96107443 A CN96107443 A CN 96107443A CN 1138095 A CN1138095 A CN 1138095A
- Authority
- CN
- China
- Prior art keywords
- incubator
- culture
- floating member
- cover cap
- plant tissue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000338 in vitro Methods 0.000 title 1
- 230000004888 barrier function Effects 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 description 24
- 235000015097 nutrients Nutrition 0.000 description 23
- 238000000034 method Methods 0.000 description 9
- 238000012258 culturing Methods 0.000 description 8
- 230000008569 process Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 4
- 238000004161 plant tissue culture Methods 0.000 description 4
- 239000002131 composite material Substances 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 230000009972 noncorrosive effect Effects 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000010935 stainless steel Substances 0.000 description 3
- 229910001220 stainless steel Inorganic materials 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000009954 braiding Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 230000008467 tissue growth Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention relates to a culture apparatus, which comprises a transparent vessel, at least one side catheter and at least one floating unit. The transparent vessel is provided with a demountable matching cover; the side catheter is provided at least one demountable matching cover and is communicated with the lower part of the vessel through at least one channel; the floating unit is arranged inside the vessel and is provided with one bearing plate with a plurality of holes so as to support the cultured plant tissue.
Description
The present invention relates to the incubator that is used for plant tissue culture, specifically, the present invention is used in plantlet regeneration is also bred in large quantities.
At present knownly in the present technique solid and liquid nutrient medium can be used for making plantlet regeneration and in addition breeding in large quantities in vierics.But, have found that, in make the common process of plantlet regenerated with Glass Containers, use the liquid nutrient medium can be more effective, because this can be convenient to cell/tissue and absorbs more oxygen and nutrition, thereby can improve output.It is generally acknowledged that incubator known in the present technique more is applicable to solid medium.Because concerning using liquid nutrient medium, the structure of this incubator has some inherent limitation.Importantly, concerning come the culturing plants tissue with liquid nutrient medium, the pollution that the plant tissue in the culturing process is caused when not being subjected to the pollution from environment also not to be subjected to incorrect processing plant tissue in a plurality of cultivation stages.In addition, when using liquid nutrient medium, must monitor substratum termly, suitably absorb substratum to reach the purpose of culturing plants tissue thereby be convenient to plant tissue to guarantee to have the substratum of proper amt.And, in order to accomplish multistage cultivation, need in culturing process, liquid nutrient medium be changed over another kind of the composition from a kind of composition to plant tissue.When changing liquid nutrient medium, must be able to make plant tissue not contaminated.Employed common incubator is unsuitable for carrying out with liquid nutrient medium the cultivation of plant tissue in the present present technique.
The objective of the invention is a kind of incubator that is used for plant tissue culture of confession under directions, can in the different steps of plant tissue culture, use above-mentioned incubator easily, and therefore reduce contaminated possibility by changing the substratum in this incubator.
According to the present invention, a kind of incubator is provided, this incubator comprises:
One transparent container, it has dismountable at least one sidepiece conduit of cooperation cover cap, and it has a dismountable cooperation cover cap and leads to as fluid by the bottom of at least one passage and said vesse;
The said relatively wall of a container face of above-mentioned sidepiece conduit is provided with in the mode that tilts;
At least one floating member, it is arranged in the said vesse and has the plant tissue that a porous supporting plate is cultivated with supporting.
Incubator of the present invention can be cultivated this tissue in that plant tissue is had under the contaminated situation.The detachable cover cap of described container can stop pollutent to enter incubator in culturing process.In addition, the sidepiece conduit is used for the variation of liquid nutrient medium is made the predetermined height of liquid nutrient medium in necessary monitoring and/or the maintenance incubator and substratum is drawn into/discharges incubator when needed.By on each cultivation stage, all suitable liquid nutrient medium being imported incubator, plant tissue is transferred to another incubator so that this plant tissue is carried out the multistage culture requirement from an incubator thereby available same incubator is satisfied via the sidepiece conduit.The open end of sidepiece conduit also is equipped with a dismountable cover cap to enter incubator to prevent pollutent via this conduit.
Fig. 1 is the sketch chart of incubator of the present invention, and it has shown the incubator that is equipped with the cover cap and is containing floating member, and the interior support of above-mentioned floating member has porous plate;
Fig. 2 is the sectional view of above-mentioned floating member, and it has illustrated provides flange to support said porous plate near the lower end of this floating member; And
Fig. 3 is the another sectional view along the whole incubator of the AA ' line among Fig. 1, is equipped with a cover cap on this incubator.
With reference to Fig. 1, wherein, incubator (V) comprises a container (CO), and it can be cylindrical or other Any shape and have a spiral cover cap (Ca).Container (CO) is equipped with sidepiece conduit (ST), and the sidewall (WS) of the relative container of this conduit (CO) is provided with in the mode that tilts.Sidepiece conduit (ST) has a cover cap (Cp), and this cover cap is fixed on the above-mentioned sidepiece conduit removably.As shown in Figure 3, cover cap (Cp) has a barrier film, and this barrier film can be made with the syringe needle that allows syringe by the material that any suitable elasticity maybe can pierce through and insert or extract.Sidepiece conduit (ST) by be arranged on the sidewall (WS) passage (O1) and (O2) and the inside of container (CO) interlink.By at interval relation each other passage (O1) and (O2) is set, therefore, passage (O1) can allow the substratum input is added or extraction container (CO) under the situation of not pulling down the cover cap.Passage (O2) is arranged on the certain altitude place of passage (O1) top and prevents to form vacuum in sidepiece conduit (ST).Container (CO) has a floating member (F), and it also can be cylindrical or any other shape.The shape of container (CO) and floating member (F) is unimportant.Floating member (F) has a porous plate (PS).According to one embodiment of the present of invention, floating member (F) is equipped with a flange that extends radially inwardly (FL), and as shown in Figure 2, this flange has a porous plate fixed thereon (PS).In addition, as shown in Figure 3, flange (FL) can support porous plate (PS).Plant tissue is placed on the porous plate (PS), and this porous plate can make that simultaneously plant tissue can absorb the nutrition from liquid nutrient medium.Like this, the plant tissue that will be used to cultivate is placed into porous plate (PS), passes through the open end of container (CO) then and puts into floating member (F).After this, seal container (CO) so that provide an environment that does not have pollutent for plant tissue with cover cap (Ca).Can or be pressed into formula by cover cap (Ca) and container (CO) any by continuous or discrete screw thread is connected and will covers cap (Ca) and be installed on the container (CO).In container (CO), provide culturing plants to organize required liquid nutrient medium by sidepiece conduit (ST) and path (O1).In case liquid nutrient medium has occupied the container (CO) of floating member (F) below, floating member (F) will rise and also float on the liquid medium within.The height of liquid nutrient medium remains on below the highest upper limit.Therefore, its height will be lower than the height of second passage (O2).The transparency of container (CO) and sidepiece conduit (ST) also helps to monitor frequently the height of the liquid nutrient medium in the container (CO).In the process of culturing plants tissue, plant tissue can absorb liquid nutrient medium, and therefore, owing to absorbed liquid nutrient medium, the height of liquid nutrient medium can begin to descend.Can observe the decline of the interior liquid nutrient medium height of container (CO), thereby, can be by keep the height of this substratum via the new liquid nutrient medium of sidepiece conduit (ST) input.Equally,, change liquid nutrient medium if desired, can effectively utilize then that sidepiece conduit (ST) is extracted out and/or the required liquid nutrient medium of input plant tissue growth carrying out the multistage during culturing process.Sidepiece conduit (ST) is equipped with detachable cooperation cover cap (Cp).The cover cap (Cp) of one most preferred embodiment has a barrier film (M), this barrier film make by rubber or any material that pierces through so that via sidepiece conduit (ST) with substratum input pod (CO).Dismountable cooperation cover cap (Cp) has the part or be contained on the top of sidepiece conduit in the mode of press-fit of being threaded that is connected in the sidepiece conduit.After being bearing in plant tissue on the porous plate (PS),, can import, dose or extract out substratum by needle pierces barrier film (M) with syringe in order to keep the contamination-free environment in the container (CO).
As shown in Figures 2 and 3, floating member (F) comprises an inner wall (F1), and it and outside wall (F2) are separately so that provide necessary strength for floating member (F).
Cover cap (Ca) and the formula that is pressed into (CP) are threaded and partly can have continuous and/or discontinuous screw thread and enter incubator (CO) to prevent pollutent.Best is that as shown in Figure 3, cover cap (Ca) is equipped with ramped bottom surface (SL) radially inside and that extend downwards.This ramped bottom surface can prevent the condensing droplet of edge cover cap wall joint delay and/or the pollution that these drops fall to being caused along the container wall.
Clearly, as the illustrated the present invention of the accompanying drawing of reference most preferred embodiment for columniform incubator and sidepiece conduit thereof can have any other shape and structure, can be leg-of-mutton, circular, quadrate, orthogonal, oval-shaped and polygonal.And the shape of floating member and porous bearing plates (PS) also can change to some extent according to the shape and the structure of said vesse.Said vesse and sidepiece conduit can be selectively by the autoclaving done such as glass, polymeric material and stainless steel/can make by germ-resistant transparent, translucent non-corrosive metal/nonmetallic composite.Floating member can have the sidewall of solid or hollow and be made by the non-corrosive metal/nonmetallic composite such as stainless steel and polymeric material.Said center support structure can form and can be made of the non-corrosive metal/nonmetallic composite such as glass fibre, polymer materials or stainless steel selectively by the mode of perforation/porose/braiding is molded.It is whole that this supporting structure can become with floating member, also can be a kind ofly to be suitable for being bearing in the absolute construction on the flange that floating member extends radially inwardly or to be arranged on similar matching block on the floating member.
In addition, can make tangible improvement, should determine scope of the present invention according to the disclosure of invention being made obvious improved possibility to above-mentioned incubator of the present invention.This respect should be noted that culture vessel of the present invention can have more than one sidepiece conduit rather than has an illustrated in the aforementioned embodiment sidepiece conduit.
Claims (10)
1. incubator, this incubator comprises:
One transparent container, it has a dismountable cooperation cover cap;
At least one sidepiece conduit, it has a dismountable cooperation cover cap, and leads to as fluid by the bottom of at least one passage and said vesse;
One porous bearing plates, it is supporting the plant tissue of being cultivated.
2. incubator as claimed in claim 1, it is characterized in that, the sidewall of said vesse has one first opening, and one second opening is interval in this first opening and is positioned at the certain altitude place of this first opening top, and above-mentioned two openings all lead to as fluid with aforementioned sidepiece conduit.
3. incubator as claimed in claim 1 is characterized in that, said floating member has a flange and a porous bearing plates that extends radially inwardly.
4. incubator as claimed in claim 3 is characterized in that said supporting plate is bearing on the said flange.
5. incubator as claimed in claim 3 is characterized in that said supporting plate is fixed on the said flange.
6. incubator as claimed in claim 1 is characterized in that said floating member comprises an inner and outer wall, and inwall is interval in outer wall.
7. incubator as claimed in claim 1 is characterized in that, the cover hatband of above-mentioned sidepiece conduit has a barrier film.
8. incubator as claimed in claim 1 is characterized in that, downward surface in the cover hatband of said vesse is oriented.
9. as any one described incubator of claim 1-8, it is characterized in that it is as indicated above basically.
10. as any one described incubator of claim 1-8, it is characterized in that it is basically as above-mentioned any one accompanying drawing is illustrated.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN546CA1995 IN183604B (en) | 1995-05-16 | 1995-05-16 | |
| IN546/95 | 1995-05-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1138095A true CN1138095A (en) | 1996-12-18 |
Family
ID=11083404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96107443A Pending CN1138095A (en) | 1995-05-16 | 1996-05-15 | Present invention relates to culture vessel for plattissue culture and is particularly useful for in vitro restoration and mass propagation of plantlets |
Country Status (9)
| Country | Link |
|---|---|
| JP (1) | JP3035384U (en) |
| CN (1) | CN1138095A (en) |
| AU (1) | AU4443496A (en) |
| CA (1) | CA2176615A1 (en) |
| DE (1) | DE19619114A1 (en) |
| FR (1) | FR2734280A1 (en) |
| GB (1) | GB2301374A (en) |
| IN (1) | IN183604B (en) |
| NL (1) | NL1003139C1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100399883C (en) * | 2005-04-15 | 2008-07-09 | 中国科学院沈阳应用生态研究所 | Plant tissue culturing liquid container |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2980867B2 (en) * | 1996-06-18 | 1999-11-22 | 明一 廖 | Cell culture method and culture device |
| US6794184B1 (en) | 1998-01-19 | 2004-09-21 | Ulrich Mohr | Culturing device and method for culturing cells or tissue components |
| DE19801763C2 (en) * | 1998-01-19 | 1999-10-28 | Ulrich Mohr | Culture device and method for culturing cells or tissue components |
| DE102004024834A1 (en) * | 2004-05-19 | 2006-01-12 | Universität Rostock | Apparatus for conducting a liquid-air culture of epithelium |
| DE102005002938B4 (en) * | 2004-11-08 | 2006-12-28 | Minuth, Will, Prof. Dr. | Method for supplying cells or tissues |
| DE102006043656B4 (en) | 2006-09-18 | 2023-08-10 | Pieter Van Weenen & Co. Gmbh The House Of Innovation | Gassing device and system |
| NL2012922B1 (en) * | 2014-05-30 | 2016-06-09 | Ccm Beheer Bv | Container for culturing organisms, method for monitoring the culturing of organisms inside said container, and monitoring system. |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4912058A (en) * | 1989-04-27 | 1990-03-27 | Becton, Dickinson And Company | Roller bottle |
-
1995
- 1995-05-16 IN IN546CA1995 patent/IN183604B/en unknown
-
1996
- 1996-02-09 AU AU44434/96A patent/AU4443496A/en not_active Abandoned
- 1996-05-10 GB GB9609762A patent/GB2301374A/en not_active Withdrawn
- 1996-05-11 DE DE19619114A patent/DE19619114A1/en not_active Withdrawn
- 1996-05-14 CA CA002176615A patent/CA2176615A1/en not_active Abandoned
- 1996-05-15 FR FR9606094A patent/FR2734280A1/en active Pending
- 1996-05-15 JP JP1996004151U patent/JP3035384U/en not_active Expired - Lifetime
- 1996-05-15 NL NL1003139A patent/NL1003139C1/en not_active IP Right Cessation
- 1996-05-15 CN CN96107443A patent/CN1138095A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100399883C (en) * | 2005-04-15 | 2008-07-09 | 中国科学院沈阳应用生态研究所 | Plant tissue culturing liquid container |
Also Published As
| Publication number | Publication date |
|---|---|
| DE19619114A1 (en) | 1996-12-05 |
| NL1003139C1 (en) | 1996-11-19 |
| GB9609762D0 (en) | 1996-07-17 |
| GB2301374A (en) | 1996-12-04 |
| AU4443496A (en) | 1996-11-28 |
| CA2176615A1 (en) | 1996-11-17 |
| IN183604B (en) | 2000-03-04 |
| JP3035384U (en) | 1997-03-18 |
| FR2734280A1 (en) | 1996-11-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4296205A (en) | Cell culture and continuous dialysis flask and method | |
| CA2113154C (en) | Culture vessel | |
| EP0239697B1 (en) | Apparatus for growing tissue cultures in vitro | |
| CN1329501C (en) | Culture dish and bioreactor system | |
| EP1502946B1 (en) | Solid state fermenter | |
| US20080076170A1 (en) | Cell culture insert and cell culture vessel | |
| US3448011A (en) | Sterile filter | |
| CA2182403A1 (en) | Plant Propagation System and Method | |
| CN208776708U (en) | A kind of microalgae sterile culture device suitable for mixotrophic cultivation | |
| CN203467354U (en) | Water-planting device for tissue culture seedlings | |
| CN1138095A (en) | Present invention relates to culture vessel for plattissue culture and is particularly useful for in vitro restoration and mass propagation of plantlets | |
| IL104385A (en) | Method and apparatus for growing biomass particles | |
| US5316945A (en) | Cell carrier arrangement | |
| EP0638640A2 (en) | Culture vessel | |
| CN207383196U (en) | A kind of forest seedling hydroponic device | |
| EP3069591B1 (en) | Bioreactor for in vitro plant culture | |
| CN108641909A (en) | A kind of microalgae sterile culture device and cultural method suitable for mixotrophic cultivation | |
| US10633628B1 (en) | Systems for cultivating and dewatering algae | |
| CN210314233U (en) | Device for extracting bacteria from plant species | |
| JP3780406B2 (en) | Methane fermentation equipment | |
| CN216712123U (en) | Ventilative digestion culture dish | |
| DE102004029709A1 (en) | Device for culturing cells in a vessel, useful e.g. for preparation of antibodies, comprising an oscillatory stirrer, membrane basket, containing carrier, and gasification membrane on the outside of the basket | |
| CN214373439U (en) | Sample section device for biological experiments | |
| CN215627902U (en) | Efficient chlorella-like culture device | |
| CN220597521U (en) | Porous cell culture plate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |