CN100399883C - Plant tissue culturing liquid container - Google Patents
Plant tissue culturing liquid container Download PDFInfo
- Publication number
- CN100399883C CN100399883C CNB2005100462603A CN200510046260A CN100399883C CN 100399883 C CN100399883 C CN 100399883C CN B2005100462603 A CNB2005100462603 A CN B2005100462603A CN 200510046260 A CN200510046260 A CN 200510046260A CN 100399883 C CN100399883 C CN 100399883C
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- container
- ventail
- plant tissue
- tissue culturing
- opening
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a cultivation container for plant tissue liquid. The cultivation container has a transparent body, and the top is provided with a cover with an air inlet; the bottom of the container is provided with an opening which can be connected with external equipment controlling liquid surface by a conducting pipe; the bottom inside the container takes the opening as a center to be provided with a supporting object in a ring mode, and a supporting plate is placed on the supporting object. The present invention improves the growth condition of plants and improves the domestication survival rate of plants. The present invention can prevent the problems of the vitrification of the plants, malformed seedlings, etc., and realize the semi-automation of a cultivation process for the plants.
Description
Technical field
The present invention relates to a kind of culture vessel that is used for biological technical field, specifically plant tissue culturing liquid container.
Background technology
The characteristics of current plant tissue culture technique are that labour intensity is big, automaticity is low, solid culture, cost height, and this also is the reason of restriction tissue culture commercial application.Compare with solid culture medium, liquid nutrient medium has its unique advantages, and at first it provides more consistent condition of culture, and is balanced in nutrition, and nutrient is easy to be absorbed by explant, simultaneously, does not shift out culture and just is easy to change liquid nutrient medium.Though liquid nutrient medium has been compared many advantages with solid culture medium, to cultivate in the long-term liquid medium within of culture, problems such as vitrifying, lopsided seedling can appear.
Summary of the invention
The object of the present invention is to provide a kind of plant tissue culturing liquid container, the while can be realized liquid culture, throughput is adjustable and incubation is semi-automatic.
For achieving the above object, the technical solution used in the present invention is:
Comprise a container, hyalosome structure, its top is the lid with air inlet, bottom at described container is provided with opening, its by conduit with can the external equipment that liquid level is controlled be linked to each other, the bottom in the container is the center with the opening, be equipped with supporter, place gripper shoe on the supporter.
Above support and container can be structure as a whole; The interior end of described container and the junction of its inwall are slick and sly shape, and outer surface central part of the interior end outwards protrudes, and are infundibulate; Have air filter in described lid inside, its concrete structure is that wherein the 1st ventail and the 2nd ventail are loose structure from stacked successively inwards filter membrane, the 1st ventail and the 2nd ventail of being equipped with of air inlet; Lid inside has internal thread, and the edge of the 1st ventail has the external screw thread that is meshed with internal thread; The middle part of the 1st ventail has a threaded post, and the middle part of the 2nd ventail has a perforate that can be threaded with threaded post, also is provided with clamping cap on threaded post; The end that described conduit can link to each other with external equipment is installed a cover cap with removably; Be provided with an annular base at container bottom, establish a recess at least at its annular position.
The present invention has the following advantages:
1. realized the semi-automation of plant incubation.Because the present invention is provided with opening in bottom of culture vessel, culturing liquid container can be under automation controlled condition, do not take out culture, by the required nutrient solution of bottom conduit input culture different phase, realization is to the cultivation of different culture different phases, link to each other with outside automatic control equipment by conduit, can realize the semi-automation of incubation, thereby can greatly reduce the input of human and material resources;
2. improved the domestication survival rate of plant.Because of the present invention covering has air filter, not only improved aeration condition, stop assorted bacterium to enter, and help the growth of culture; The present invention simultaneously also provides throughput adjustable lid, according to the difference of culture desired gas amount, regulates suitable throughput, helps the growth of culture; In addition, because good throughput is arranged, tissue cultivating seedling is grown in oxygen half different oxygen environment half from the beginning, helps the domestication of later stage from the heterotrophism to the autotrophy, can improve the domestication survival rate greatly.
3. can prevent the appearance of problems such as plant vitrifying, lopsided seedling.Owing to be provided with supporter in the transparent vessel of the present invention, can support culture, be unlikely to make culture to be immersed in the liquid nutrient medium, container bottom is connected with outside automatic control equipment by conduit simultaneously, can control the liquid level in the container, realize intermittently providing nutrient, avoid the appearance of vitrifying seedling, lopsided seedling;
4. save resource, reduced cost.The present invention adopts liquid nutrient medium, has not only save agar, and liquid nutrient medium can also cycling and reutilization, has positive effect aspect the resource reducing cost and save.
Description of drawings
Fig. 1 is a structural representation of the present invention;
Fig. 2 is the assembling schematic diagram of the present invention's lid;
Fig. 3 is the vertical view of the present invention's the 1st ventail;
Fig. 4 is the vertical view of the present invention's the 2nd ventail;
Fig. 5 is the vertical view of clamping cap of the present invention.
Embodiment
The invention will be further described below in conjunction with drawings and Examples.
As shown in Figure 1, the present invention includes a container 2, it is made by transparent high temperature resistant, high pressure material, and have one the lid 1, with container 2 tops for being threaded, this lid has air inlet 15; Container 2 bottoms are provided with opening 5, and it links to each other with conduit 3, and conduit is equipped with cover cap 3a, and this cover cap 3a is installed on the conduit removably, cover cap 3a are unloaded conduit 3 is connected with outside automatic control equipment, by conduit 3 transportation culture fluids; Supporter 4 is arranged in the transparent vessel 2, and ring is located at around the opening 5, is fixed as one with container 2, and transparent vessel 2 is had the reinforcing supporting role, and support 4 shapes are not limit, but will guarantee that gripper shoe 4a can stablize the also maintenance level that is placed on the support 4; There is the gripper shoe 4a of a porous supporter 4 tops; In the present embodiment, this supporter 4 supports with the solid thing of two symmetries, and the center is continuous, leaves and opening 5 sizable mouths; Chamber wall 7 is slick and sly shape with inboard, container bottom 8 junctions, and is not residual herein with assurance liquid, and container bottom 8 outwards protrudes, and is infundibulate; When liquid when opening 5 flows into, make liquid can be dispersed in container 2 bottoms; In container 2 bottoms one annular base 6 is installed also, its annular position has recess, to make things convenient for being connected of conduit 3 and external control devices.
As shown in Figure 2, lid has an air inlet 15 on 1, and lid 1 and air filter are for being threaded; Described air filter is to be fixed together by threaded post 11a by two the 1st ventails 9 with holes and the 2nd ventail 10, and compress with clamping cap 11, be placed with filter membrane 12 between ventail 9 and lid 1, this filter membrane 12 is a native fiber, also can be synthetic fibers; The 1st ventail 9 and the 2nd ventail 10 are loose structure, can relatively rotate; During use, the dislocation size that elder generation regulates the 1st ventail 9 and the 2nd ventail 10 air-vents according to the required throughput of culture, then filter membrane 12 is placed on the 1st ventail 9, is screwed in by the external screw thread 13 on the 1st ventail 9 and covers on 1, compress the 1st ventail 9 and the 2nd ventail 10 with clamping cap 11; The gripper shoe 4a of porous is placed on the support 4, again culture is placed on the gripper shoe 4a, container 2 is screwed on covered 1, behind the autoclaving, lay down cover cap 3a, conduit 3 is linked to each other with outside automatic control equipment, input or output culture fluid, and control liquid level automatically, carry out intermittence or continuous culture.Stage difference according to culture is cultivated inputs or outputs culture desired nutritional liquid by conduit 3, and need not culture be taken out, and has saved the man power and material.Described clamping cap 11 is fixed together the 1st, 2 ventails 9,10 by threaded post 11a.
From above-mentioned explanation as can be seen, essence of the present invention provides a kind of reasonable in design, the purpose of can realize liquid culture again simultaneously, throughput is adjustable, semi-automation is cultivated.The said parts of the present invention are not limited to above-mentioned concrete shape and structure.
Claims (4)
1. plant tissue culturing liquid container, this container is a hyalosome structure, the top is the lid (1) with air inlet, it is characterized in that: the bottom (8) in described container (2) is provided with opening (5), opening (5) links to each other with the external equipment that liquid level is controlled by conduit (3), bottom in the container (2) is the center with opening (5), is equipped with supporter (4), goes up at supporter (4) and places gripper shoe (4a); Bottom (8) in container (2) is provided with an annular base (6), establishes a recess at least in this annular base (6) lower end; Container bottom (8) is slick and sly shape with the inboard, junction of chamber wall (7); Has air filter in described lid (1) inside, this air filter concrete structure is from inwardly stacked successively filter membrane (12), the 1st ventail (9) and the 2nd ventail (10) of being equipped with of air inlet (15), and wherein the 1st ventail (9) is loose structure with the 2nd ventail (10); Lid (1) inside has internal thread (14), and the edge of the 1st ventail (9) has the external screw thread (13) that is meshed with internal thread (14); The middle part of the 1st ventail (9) has a threaded post (11a), and the middle part of the 2nd ventail (10) has a perforate that is threaded with threaded post (11a), also is provided with clamping cap (11) on threaded post (11a).
2. by the described plant tissue culturing liquid container of claim 1, it is characterized in that: above support (4) is structure as a whole with container (2).
3. by the described plant tissue culturing liquid container of claim 1, it is characterized in that: described container bottom (8) is slick and sly shape with the inboard, junction of chamber wall (7), and container bottom (8) central part outwards protrudes, and is infundibulate.
4. by the described plant tissue culturing liquid container of claim 1, it is characterized in that: the end that described conduit (3) links to each other with external equipment is installed a cover cap (3a) with removably.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100462603A CN100399883C (en) | 2005-04-15 | 2005-04-15 | Plant tissue culturing liquid container |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100462603A CN100399883C (en) | 2005-04-15 | 2005-04-15 | Plant tissue culturing liquid container |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1846478A CN1846478A (en) | 2006-10-18 |
| CN100399883C true CN100399883C (en) | 2008-07-09 |
Family
ID=37076410
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100462603A Expired - Fee Related CN100399883C (en) | 2005-04-15 | 2005-04-15 | Plant tissue culturing liquid container |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100399883C (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2239134Y (en) * | 1996-03-08 | 1996-11-06 | 陈贯 | Plant culture vessel having automatic water supply |
| CN1138095A (en) * | 1995-05-16 | 1996-12-18 | 印度技术研究院 | Present invention relates to culture vessel for plattissue culture and is particularly useful for in vitro restoration and mass propagation of plantlets |
| CN2466900Y (en) * | 2001-03-21 | 2001-12-26 | 中国科学院遗传研究所 | Culture container |
-
2005
- 2005-04-15 CN CNB2005100462603A patent/CN100399883C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1138095A (en) * | 1995-05-16 | 1996-12-18 | 印度技术研究院 | Present invention relates to culture vessel for plattissue culture and is particularly useful for in vitro restoration and mass propagation of plantlets |
| CN2239134Y (en) * | 1996-03-08 | 1996-11-06 | 陈贯 | Plant culture vessel having automatic water supply |
| CN2466900Y (en) * | 2001-03-21 | 2001-12-26 | 中国科学院遗传研究所 | Culture container |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1846478A (en) | 2006-10-18 |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080709 Termination date: 20100415 |