CN109362548A - The method of comprehensive induction tea tree bud-leaf purple - Google Patents
The method of comprehensive induction tea tree bud-leaf purple Download PDFInfo
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- CN109362548A CN109362548A CN201811379822.XA CN201811379822A CN109362548A CN 109362548 A CN109362548 A CN 109362548A CN 201811379822 A CN201811379822 A CN 201811379822A CN 109362548 A CN109362548 A CN 109362548A
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- tea tree
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention particularly relates to it is a kind of by comprehensive utilization simulation natural ecological environment change process induce tea tree newly slightly blade occur purpleization method.The present invention is on the basis of purpleization phenomenon gene chemical synthesis expression regulation occurs for apparent tea tree bud-leaf, the variation of simulating natural environment, influences the regulation and control model of tea tree bud-leaf anthocyanin key gene express spectra by regulating and controlling light quality and light intensity；Promote the expression of anthocyanin synthesis key gene using low temperature induction；Control the induction tea tree anthocyanin route of synthesis structural gene up-regulation of n-p-k element content, nitrogen in its blade is influenced in blade surface sprinkling sucrose solution, the appropriate drought stress of progress simultaneously to synthesize, it is increased so as to cause the accumulation of anthocyanin in tea tree bud-leaf, tea tree bud-leaf generates purpleization phenomenon.
Description
Technical field
The invention belongs to tree plant cultivation breeding fields, and in particular to the side of purpleization occurs to a kind of comprehensive induction tea tree bud-leaf
Method.
Technical background
Tea tree " purple bud " is a kind of special Tea Germplasm, relatively common in group's kind tea place, tea tree purple
Mainly due to the gene mutation that weather and seasonal variations cause, in the synthesis shadow of the environmental factors such as low temperature, illumination, fertilizer nutrient
Under sound, the expression up-regulation of anthocyanin synthase gene, while the chlorophyll in bud-leaf is converted into anthocyanidin, research shows that tea tree bud-leaf
The accumulation of purpleization and anthocyanidin in bud-leaf is closely related.Contained anthocyanidin accounts for about dry matter and contains in general tea tree Purple tea shoots
The 0.5%~1% of amount, much higher than green bud-leaf.As what purple young tea leaves tree germplasm innovation was studied gos deep into, main component
Medical health care function is gradually elucidated with, especially the stronger anti-oxidation function of anthocyanidin.Tea tree Purple tea shoots are as a kind of characteristic
Tea tree breed resource, the breeding of purple bud tea tree breed and high anthocyanidin tea product development and utilization more and more paid close attention to by people, phase
Research is closed to be rapidly developed in recent years.
Anthocyanidin is accumulated in summer and autumn by own growth and development state and extraneous nature in most of tea tree breed
The influence of the factors such as condition illumination, temperature, nutritional condition, to show different degrees of purple bud phenomenon.With molecular engineering
Horizontal deepens continuously, and having verified the genes such as CHS, CHI, F3H, DFR, ANS, ANR at present is that anthocyanin synthesizes in Purple tea shoots
Key gene, and the illumination of the different colours such as purple light, feux rouges, blue light, low-temperature treatment appropriate, n-p-k element low water
Flat induction, drought stress appropriate play a part of just regulating and controlling to the expression of these anthocyanin synthase genes.
However the breeding of tea tree purple kind at present is still formed by traditional field systematic breeding expansion is numerous, the breeding period
It is long, Breeding Process is cumbersome, field work amount is big etc., and inconvenient factor becomes the drawbacks of can not overcoming for a long time in breeding work, one
Determine the breeding that purpleization tea tree breed is significantly limited in degree.
Summary of the invention
The present invention utilizes illumination, temperature, n-p-k element level, drought stress etc. using Different Tea Varieties as raw material
The change of environmental factor influences tea tree by regulating and controlling the synthesis of anthocyanin key gene CHS, CHI, F3H, DFR, ANS, ANR
The accumulation of anthocyanidin content in bud-leaf contributes to realize the purpose of bud-leaf purpling for the popularization of tea tree purple kind, from
And it achieves the object of the present invention.
To achieve the goals above, the technical solution adopted by the present invention is that, it is a kind of it is comprehensive induction tea tree bud-leaf purple side
Method, it is characterized in that comprising the steps of:
(1) selection and water planting of tea tree branch
It chooses and has fresh tender leaf or axillary bud, the consistent tea tree stalwartness branch of growing way carries out 1~2d of water planting using pure water.
(2) drought stress is handled
Tea shoot in water planting is drained away the water, in its natural state, 15~35h is placed, carries out drought stress processing.
(3) treatment with ultraviolet light
By drought stress, treated that branch is placed in ultraviolet light incubator, ultraviolet wavelength 254nm, radiation intensity range
For 1.1~1.9w/m2, daily irradiation time is 4~5h, and the treatment with ultraviolet light period is 5~6d.
(4) sucrose solution is handled
100ml~200ml/m is pressed to tea tree branch leaf surface2Amount uniformly spray the sucrose solution of 0.05g/L, then
Allow its in the raw under.
(5) Solution culture method
Configure certain density low nitrogen low-phosphorous low potassium nutrition liquid (N < 60mmol/L, P < 45mmol/L, K+< 50mmol/
L), branch is inserted into configured nutrient solution and is cultivated.
Nutrient solution matches as follows in detail:
| Element | Molecular formula | Concentration (mmol/L) |
| NH4 + | (NH4)2SO4 | 10 |
| NO3 - | Ca(NO3)2.4H2O | 10 |
| P | KH2PO4 | 3.1 |
| K | KH2PO4.K2SO4 | 20 |
| Ca | CaCl2.H2O | 30 |
| Mg | MgSO4.7H2O | 25 |
| Fe | Fe-EDTA | 0.35 |
| B | H3BO3 | 0.1 |
| Mn | MnSO4.4H2O | 1.0 |
| Zn | ZnSO4.7H2O | 0.1 |
| Cu | CuSO4.5H2O | 0.025 |
| Mo | Na2MoO4.2H2O | 0.05 |
| Al | Al2(SO4)3.16-18.2H2O | 10.8 |
(6) low-temperature treatment
The branch cultivated in nutrient solution will be put into be placed in artificial climate incubator, carry out low-temperature treatment, treatment temperature is
Between 10 DEG C~20 DEG C, the period is 8~10d, and after process cycle, purpleization phenomenon is gradually presented in tea tree branch tender leaf.
(7) cuttage is expanded numerous
By the processed branch that purpleization has been presented, DB42/T181-1999 Clonal tea shoot is bred according to national standards
Technical regulation standard carries out cuttage in tea place and expands numerous and management.
The invention has the following advantages that
The present invention is on the basis of purpleization phenomenon gene chemical synthesis expression regulation occurs for apparent tea tree bud-leaf, by simulating oneself
The variation of right environment regulates and controls light quality and light intensity to influence the regulation and control model of tea tree bud-leaf anthocyanin key gene express spectra；Benefit
Promote the expression of anthocyanin synthesis key gene with low temperature induction；Control the induction tea tree anthocyanin synthesis of n-p-k element content
Pathway structure gene upregulation, while sucrose solution is sprayed in blade surface, it influences nitrogen in its blade and synthesizes, so as to cause tea tree
The accumulation of anthocyanin increases in bud-leaf, and tea tree bud-leaf generates purpleization phenomenon.Achieve the purpose that purpleization, the hair is presented in tea tree bud-leaf
It is bright to can break through the long-term predicament for relying on traditional field line breeding, solve that breeding cycle is long, Breeding Process is cumbersome, field work
The problem brought by factor that brings inconvenience such as big is measured, is contributed for the progress of Tea Breeding cause.
Detailed description of the invention:
Fig. 1 is to meet comparison diagram before and after white tea tree breed bud-leaf purpleization
Fig. 2 is comparison diagram before and after the tea tree breed bud-leaf purpleization of Fuding
Fig. 3 is comparison diagram before and after No. 10 tea tree breed bud-leaf purpleizations of tin tea
Specific embodiment:
The present invention is described in detail for each embodiment shown in reference to the accompanying drawing.
Case study on implementation 1:
(1) selection and water planting of tea tree branch
It chooses 10 and has fresh tender leaf, the consistent healthy and strong branch for meeting white tea tree breed of growing way carries out water using pure water
Train 1d.
(2) drought stress is handled
Tea shoot in water planting is drained away the water, places 15h in its natural state, carries out drought stress processing.
(3) treatment with ultraviolet light
By drought stress, treated that branch is placed in the ultraviolet light incubator of model UVC-254, ultraviolet wavelength
254nm, radiation intensity range are 1.1w/m2, daily irradiation time is 4h, and the treatment with ultraviolet light period is 5d.
(4) sucrose solution is handled
100ml/m is pressed to tea tree branch leaf surface2Amount uniformly spray the sucrose solution of 0.05g/L, then allow at it
In under natural conditions.
(5) Solution culture method
Configure certain density low nitrogen low-phosphorous low potassium nutrition liquid (N < 60mmol/L, P < 45mmol/L, K+< 50mmol/
L), branch is inserted into configured nutrient solution and is cultivated.
Nutrient solution matches as follows in detail:
| Element | Molecular formula | Concentration (mmol/L) |
| NH4 + | (NH4)2SO4 | 10 |
| NO3 - | Ca(NO3)2.4H2O | 10 |
| P | KH2PO4 | 3.1 |
| K | KH2PO4.K2SO4 | 20 |
| Ca | CaCl2.H2O | 30 |
| Mg | MgSO4.7H2O | 25 |
| Fe | Fe-EDTA | 0.35 |
| B | H3BO3 | 0.1 |
| Mn | MnSO4.4H2O | 1.0 |
| Zn | ZnSO4.7H2O | 0.1 |
| Cu | CuSO4.5H2O | 0.025 |
| Mo | Na2MoO4.2H2O | 0.05 |
| Al | Al2(SO4)3.16-18.2H2O | 10.8 |
(6) low-temperature treatment
The branch cultivated in nutrient solution will be put into be placed in the artificial climate incubator of model LRG-250A-L, carried out low
Temperature processing, treatment temperature are 10 DEG C, and after period 8d, 8d, purpleization phenomenon is gradually presented in tea tree branch tender leaf.
(7) cuttage is expanded numerous
By the processed branch that purpleization has been presented, DB42/T181-1999 Clonal tea shoot is bred according to national standards
Technical regulation standard carries out cuttage in tea place and expands numerous and management.
Case study on implementation 2:
(1) selection and water planting of tea tree branch
It chooses 10 and has axillary bud, the healthy and strong branch of the consistent Fuding tea tree breed of growing way carries out water planting using pure water
1.5d。
(2) drought stress is handled
Tea shoot in water planting is drained away the water, places 20h in its natural state, carries out drought stress processing.
(3) treatment with ultraviolet light
By drought stress, treated that branch is placed in the ultraviolet light incubator of model UVC-254, ultraviolet wavelength
254nm, radiation intensity range are 1.2w/m2, daily irradiation time is 4.5h, and the treatment with ultraviolet light period is 5.5d.
(4) sucrose solution is handled
150ml/m is pressed to tea tree branch leaf surface2Amount uniformly spray the sucrose solution of 0.05g/L, then allow at it
In under natural conditions.
(5) Solution culture method
Configure certain density low nitrogen low-phosphorous low potassium nutrition liquid (N < 60mmol/L, P < 45mmol/L, K+< 50mmol/
L), branch is inserted into configured nutrient solution and is cultivated.
Nutrient solution matches as follows in detail:
| Element | Molecular formula | Concentration (mmol/L) |
| NH4 + | (NH4)2SO4 | 10 |
| NO3 - | Ca(NO3)2.4H2O | 10 |
| P | KH2PO4 | 3.1 |
| K | KH2PO4.K2SO4 | 20 |
| Ca | CaCl2.H2O | 30 |
| Mg | MgSO4.7H2O | 25 |
| Fe | Fe-EDTA | 0.35 |
| B | H3BO3 | 0.1 |
| Mn | MnSO4.4H2O | 1.0 |
| Zn | ZnSO4.7H2O | 0.1 |
| Cu | CuSO4.5H2O | 0.025 |
| Mo | Na2MoO4.2H2O | 0.05 |
| Al | Al2(SO4)3.16-18.2H2O | 10.8 |
(6) low-temperature treatment
The branch cultivated in nutrient solution will be put into be placed in the artificial climate incubator of model LRG-250A-L, carried out low
Temperature processing, treatment temperature are 15 DEG C, and purpleization phenomenon is gradually presented in tea tree branch tender leaf after period 9d, 9d.
(7) cuttage is expanded numerous
By the processed branch that purpleization has been presented, DB42/T181-1999 Clonal tea shoot is bred according to national standards
Technical regulation standard carries out cuttage in tea place and expands numerous and management.
Case study on implementation 3:
(1) selection and water planting of tea tree branch
Choose 10 have fresh tender leaf, the healthy and strong branch of growing way No. 10 tea tree breeds of consistent tin tea, using pure water into
Row water planting 2d.
(2) drought stress is handled
Tea shoot in water planting is drained away the water, places 35h in its natural state, carries out drought stress processing.
(3) treatment with ultraviolet light
By drought stress, treated that branch is placed in the ultraviolet light incubator of model UVC-254, ultraviolet wavelength
254nm, radiation intensity range are 1.9w/m2, daily irradiation time is 5h, and the treatment with ultraviolet light period is 6d.
(4) sucrose solution is handled
200ml/m is pressed to tea tree branch leaf surface2Amount uniformly spray the sucrose solution of 0.05g/L, then allow at it
In under natural conditions.
(5) Solution culture method
Configure certain density low nitrogen low-phosphorous low potassium nutrition liquid (N < 60mmol/L, P < 45mmol/L, K+< 50mmol/
L), branch is inserted into configured nutrient solution and is cultivated.
Nutrient solution matches as follows in detail:
| Element | Molecular formula | Concentration (mmol/L) |
| NH4 + | (NH4)2SO4 | 10 |
| NO3 - | Ca(NO3)2.4H2O | 10 |
| P | KH2PO4 | 3.1 |
| K | KH2PO4.K2SO4 | 20 |
| Ca | CaCl2.H2O | 30 |
| Mg | MgSO4.7H2O | 25 |
| Fe | Fe-EDTA | 0.35 |
| B | H3BO3 | 0.1 |
| Mn | MnSO4.4H2O | 1.0 |
| Zn | ZnSO4.7H2O | 0.1 |
| Cu | CuSO4.5H2O | 0.025 |
| Mo | Na2MoO4.2H2O | 0.05 |
| Al | Al2(SO4)3.16-18.2H2O | 10.8 |
(6) low-temperature treatment
The branch cultivated in nutrient solution will be put into be placed in the artificial climate incubator of model LRG-250A-L, carried out low
Temperature processing, treatment temperature are 20 DEG C, and purpleization phenomenon is gradually presented in tea tree branch tender leaf after period 10d, 10d.
(7) cuttage is expanded numerous
By the processed branch that purpleization has been presented, DB42/T181-1999 Clonal tea shoot is bred according to national standards
Technical regulation standard carries out cuttage in tea place and expands numerous and management.
Claims (1)
1. a kind of method of comprehensive induction tea tree bud-leaf purple, it is characterized in that comprising the steps of:
(1) selection and water planting of tea tree branch
It chooses and has fresh tender leaf or axillary bud, the consistent tea tree stalwartness branch of growing way carries out 1~2d of water planting using pure water.
(2) drought stress is handled
Tea shoot in water planting is drained away the water, in its natural state, 15~35h is placed, carries out drought stress processing.
(3) treatment with ultraviolet light
By drought stress, treated that branch is placed in ultraviolet light incubator, ultraviolet wavelength 254nm, and radiation intensity range is
1.1~1.9w/m2, daily irradiation time is 4~5h, and the treatment with ultraviolet light period is 5~6d.
(4) sucrose solution is handled
100ml~200ml/m is pressed to tea tree branch leaf surface2Amount uniformly spray the sucrose solution of 0.05g/L, then allow it
Under in the raw.
(5) Solution culture method
Configure certain density low nitrogen low-phosphorous low potassium nutrition liquid (N < 60mmol/L, P < 45mmol/L, K+< 50mmol/L), it will
Branch is inserted into configured nutrient solution and is cultivated.
Nutrient solution matches as follows in detail:
(6) low-temperature treatment
The branch cultivated in nutrient solution will be put into be placed in artificial climate incubator, carry out low-temperature treatment, treatment temperature is 10 DEG C
Between~20 DEG C, the period is 8~10d, and after process cycle, purpleization phenomenon is gradually presented in tea tree branch tender leaf.
(7) cuttage is expanded numerous
By the processed branch that purpleization has been presented DB42/T181-1999 Clonal tea shoot raising technology according to national standards
Norm standard carries out cuttage in tea place and expands numerous and management.
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| CN201811379822.XA CN109362548A (en) | 2018-11-05 | 2018-11-05 | The method of comprehensive induction tea tree bud-leaf purple |
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|---|---|---|---|
| CN201811379822.XA CN109362548A (en) | 2018-11-05 | 2018-11-05 | The method of comprehensive induction tea tree bud-leaf purple |
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| CN109362548A true CN109362548A (en) | 2019-02-22 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113785715A (en) * | 2021-09-18 | 2021-12-14 | 福建农林大学 | Method for regulating and controlling tea tree leaf color |
| CN113853964A (en) * | 2021-10-08 | 2021-12-31 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Macadamia nut breeding method combining grafting and mutagenesis |
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| CN104962565A (en) * | 2015-07-16 | 2015-10-07 | 广东省农业科学院饮用植物研究所 | Purple bud tea tree R2R3-MYB gene CsMYB2 and application thereof |
| CN105432828A (en) * | 2015-12-17 | 2016-03-30 | 中国农业科学院茶叶研究所 | Processing method for improving content of anthocyanins in purple bud tea |
| CN106480063A (en) * | 2016-08-22 | 2017-03-08 | 华南农业大学 | A kind of tea tree myb transcription factor CsAN1 and its application in regulation and control anthocyanidin metabolism |
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2018
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104962565A (en) * | 2015-07-16 | 2015-10-07 | 广东省农业科学院饮用植物研究所 | Purple bud tea tree R2R3-MYB gene CsMYB2 and application thereof |
| CN105432828A (en) * | 2015-12-17 | 2016-03-30 | 中国农业科学院茶叶研究所 | Processing method for improving content of anthocyanins in purple bud tea |
| CN106480063A (en) * | 2016-08-22 | 2017-03-08 | 华南农业大学 | A kind of tea tree myb transcription factor CsAN1 and its application in regulation and control anthocyanidin metabolism |
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| SOLFANELLI C,等: "Sucrose-specific induction of the anthocyanin biosynthetic pathway in Arabidopsis", 《PLANT PHYSIOLOGY》 * |
| 李智: "不同环境因子调控茶树紫色芽叶形成的分子机制研究", 《中国博士学位论文全文数据库农业科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113785715A (en) * | 2021-09-18 | 2021-12-14 | 福建农林大学 | Method for regulating and controlling tea tree leaf color |
| CN113853964A (en) * | 2021-10-08 | 2021-12-31 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Macadamia nut breeding method combining grafting and mutagenesis |
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Application publication date: 20190222 |