CN1093524A - Polymeric gel biological bacteria pesticide and preparation technology thereof - Google Patents
Polymeric gel biological bacteria pesticide and preparation technology thereof Download PDFInfo
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- CN1093524A CN1093524A CN 94103705 CN94103705A CN1093524A CN 1093524 A CN1093524 A CN 1093524A CN 94103705 CN94103705 CN 94103705 CN 94103705 A CN94103705 A CN 94103705A CN 1093524 A CN1093524 A CN 1093524A
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Abstract
A kind of polymeric gel biological bacteria pesticide and preparation technology belong to biopesticide insecticide and preparation technology.By active bio bacterium such as fungi by the contained weight of 500ml unit volume is that white muscardine fungi or bacterium are Bactospeine 20-30 grams, is that 20%500ml and proper amount of cross-linking agent, initator, stabilizing agent and 150-160 gram starch nutrition things are mixed mutually with its concentration of unimolecule organic matter that contains unsaturated double-bond.Its preparation technology mixes said components and content 30 minutes at 40-45 ℃, is finished product in 35 ℃ of following dryings again through moulding.This biological bacteria pesticide resistance, the field is active to prolong 2 months, and preventive effect reaches 86%, and products material is abundant, production technology is easy.
Description
A kind of polymeric gel biological bacteria pesticide and preparation technology thereof belong to biopesticide insecticide and preparation technology thereof.
The used bacterium for killing insect of biological bacteria pesticide is active stronger fungi such as white muscardine fungi and bacterium such as Bactospeine, and mostly fungi is to utilize the interior composition of pest body to be nutrition, by breeding in the larva body, parasitism the insect larva is caused death.Bacterium is eaten the back for excreting poison by insect and causes death.Existing so far recent two decades history is used in this two classes bacterium for killing insect field, and this class bacterium for killing insect is used and is the use of gymnospore bacterium always for a long time, and thalline self does not have protectiveness, meets unfavorable factors such as illumination, dehydration, drying and all will lose infection to the host.This is to wait the technical barrier that solves during use in biologic product desinsection field so far.Abroad the someone studies with the protection carrier of starch as the desinsection microbial inoculum in recent years, resists the influence of natural environment to strengthen biological bacteria.But starch is made the protection carrier and is met water and easily decompose, and does not have a repeatability.The academy of agricultural sciences, Jilin Province once initiated cultivates secondary bacterium that 2 grades of bacterium of white muscardine fungi promptly cultivate with the corn core leaf and directly takes the field and apply control of maize snout moth's larva in the corn core leaf; its result of use has had large increase; but the problem that exists is a bacterium, and the active time is short; still used by the gymnospore bacterium; the outer unprotect of bacterium; meet rainwater and easily run off, inactivations such as illumination and drying.
The objective of the invention is to solve above-mentioned the deficiencies in the prior art; provide a kind of polymeric gel to protect carrier as biological bacteria with dehydration, rehydration repeatability; thalline can be bred on gel, utilize the character of gel to make it strengthen resistance to external world, its preparation technology of polymeric gel biological bacteria pesticide agricultural chemicals of increase thalline field effective active.
The present invention realizes with following technical scheme: this polymeric gel biological bacteria pesticide is to be mixed mutually with the unimolecule organic matter 20% concentration 500ml that contains the two keys of insatiable hunger and an amount of crosslinking agent, initator, stabilizing agent and 150-160 gram starch nutrition thing by activated biological bacteria 20-30 gram by the contained weight of 500ml unit volume.Its preparation technology is the active bio bacterium 20-30 gram by the contained weight of 500ml unit volume 30 minute is become polymeric gel biological bacteria with an amount of initator, crosslinking agent, stabilizing agent and 150-160 gram starch nutrition thing 40-45 ℃ of insulation mixing with the unimolecule organic matter 20% concentration 500ml that contains unsaturated double-bond, through former beading particle, make the pearl polymerisation gel biological bacteria pesticide again in 35 ℃ of following dryings.Its preparation technology of appropriate change, can obtain the powdery polymeric gel biological bacteria pesticide, preparation technology is the unsaturated unimolecule organic matter that will contain two keys earlier, an amount of crosslinking agent, initator, stabilizing agents etc. mixed 30 minutes at 40-45 ℃, very fast formation gel, claim the RJS gel, being machined into little blob of viscose can be by 30 order meshes, again active bio bacterium such as white muscardine fungi are enlarged into three grades of bacterium of liquid step by step through PDA potato liquid nutrient medium or synthetic liquid nutrient medium, mix with above-mentioned RJS gel particle and corn starch wheat bran skin culture, cultivated 7-10 days in 26 ℃, it is finished product that the culture that obtains is crossed 40 mesh sieves in 35 ℃ of dry backs.
It is as follows that polymeric gel forms mechanism:
Polymeric gel is formed with multiple different system, and promptly owing to the monomer concentration difference, the polymerizing condition difference can form the different gels of inhaling the multiple of splashing, but various gel all has following structure, and it is as follows to form mechanism:
Earlier by monomer to propagation process:
Form imidization between the long-chain polymer, be cross-linked into network structure:
Add a certain amount of crosslinking agent in the system again, therefore following structure arranged again:
Therefore, gel shape microbial inoculum is the mixed structure of above-mentioned two kinds of different web form.The netted polymeric gel mycelia that different proportionings form all can grow; it is luxuriant that the set of dispense ratio that the present invention determines is more suitable for mycelial growth, mycelia penetrates gel growth; find out by the gel molecular structure; can the required nutrients of wrapping biological bacteria growing in the grid; corn starch and wheat bran extract; meeting can provide mycelia or required moisture content and the nutrients of bacterium spore germination after the water suction is splashed; when dry because the effect of gel moisture capacity even irreducible water; can keep the required minimum water of mycelia and spore, be that biological bacteria is protected the key point that increases the service life therefore.
The beneficial effect that the present invention had: because polymeric gel is unsaturated double-bond unimolecule organic matter, through initator, crosslinking agent, stabilizing agent etc. under certain condition the short time polymerization crosslinking reticulate gel.This gel has stronger water imbibition, and good water-retaining property except that being surrounded by a large amount of bacterium spores, also has required starch and the wheat bran nitride of thalli growth in the grid.When meeting rainwater, gel rapidly splash rise dry mycelia and spore of suction is sprouted, and when the slow dehydration of desiccant gel helps the spore and the mycelia of mycelia and sporulation dormancy, also can repeat said process when meeting rainwater.Therefore, the character that gel had is that this biological bacteria improves active key.Gel biological bacteria pesticide with the preparation of polymeric gel technology has following beneficial effect: 1, gel is suitable for multiple bacterium for killing insect, and microbial inoculum is suitable for preventing and treating nearly hundred kinds of insects.2, be example with the control corn borer, the field effective active extends to two months by three to five days of routine.3, gel has dehydration, rehydration repeatability, compares and has overcome starch and meet to decompose behind the water and do not have a technical barrier of repeatability with there being at present human starch to do the protection carrier abroad.4, be example with the control corn borer, this microbial inoculum effective drug duration is long, can shift to an earlier date dispenser at corn growth stage, has overcome present technical problem because of the tall and big dispenser difficulty of plant, welcome by the peasant.5, have than the large economy benefit: two kinds of microbial inoculums of pearl that the present invention possessed and powdery, according to present technology, production cost is as follows: a, powdery microbial inoculum are that 1.15 yuan of mu consumptions of per kilogram are 600 grams, medicament expense is that 0.69 yuan, preventive effect corn borer are 86%, and contrast exceeds 10%, by 300 kilograms of corns conversions of per mu yield, can retrieve a loss 24 kilograms/mu, pressing 0.4 yuan of conversion of per kilogram, is 9.6 yuan, and therefore the mu input-output ratio is 12.9: 1.B, pearl microbial inoculum cost are 18.1 yuan/kilogram.Every mu of usefulness 100 grams, 1.81 yuan of medicament expenses, preventive effect contrast improves 8%, on average can retrieve a loss 19.2 kilograms/mu.Amount to 7.68 yuan, input-output ratio is 4.24: 1.But the pearl microbial inoculum is more suitable in the control soil insect.
Heilongjiang Province, China northeast is a corn belt, and the annual sowing area reaches 3,300 ten thousand mu.Because the corn borer hazard area reaches 1,014 ten thousand mu, when serious mu with a toll of 10% or more and by present Prevention Technique, every year less than 4,500,000 mu.The present invention uses the polymeric gel technology, greatly improved present white muscardine fungi field control technology, can shift to an earlier date dispenser, the microbial inoculum resting period is long, can standardization, commercialization, these technological merits bring breakthrough will for Heilongjiang Province's corn borer large area control.
The beneficial effect of above 1-4 can be used the following verification experimental verification that pearl and powdery microbial inoculum are done.
Test 1, powdery and pearl microbial inoculum are to the influence of ultra violet lamp: powdery and pearl gel microbial inoculum are respectively got 1 gram dry product put the following 1.2 meters irradiation of 30W uviol lamp, be respectively 5,10,20,30 minutes, and then place 50ml liquid PDA medium to ferment 48 hours, dilute 0.1% again, the results are shown in Table 1 with the dull and stereotyped clump count that calculates of PDA.
Table 1 ultra violet lamp test * 10
3Individual
Test 2 is the test of survey living after two kinds of formulation long-term storage: powdery microbial inoculum and pearl microbial inoculum are at room temperature placed respectively and were respectively got 1 in 260 days and 256 days and restrain dry product and do following Processing Test, its method is as follows: (1) gets 1 gram dry product in the PDA liquid nutrient medium, contain sugar 2%, PH nature, 26～28 ℃ of shaken cultivation 72 hours, the microscopy counting.(2) respectively get the dried microbial inoculum of 1 gram and soaked 26 hours with excessive physiological saline respectively, in plate, cultivate collection spore and counting after 7 days for 26 ℃, the results are shown in Table 2.
Deposit under table 2 pearl and the powdery microbial inoculum room temperature to survey in 8 months and live
Above result can verify that pearl and powdery microbial inoculum were at room temperature deposited after 8 months and still have activity, and be better than conventional gymnospore.
Test 3 is the field application test.Gel microbial inoculum type once under the assistance of Heilongjiang Province's plant quarantine plant protection unit, had been done the application test of 3 years field control corn borers, and was the most obvious with 93 years field effects, the results are shown in Table 3.
Suitable as can be seen from Table 3 mu consumption is 15 gram bacterium spores, and spore content is 10
11Individual/gram.Gel pearl microbial inoculum consumption is 100 gram/mus, and the powdery microbial inoculum is 600 gram/mus.Preventive effect reaches more than 80%, reaches 86.15% in 93 years.Contrast high 10 percentage points.By field application test, pearl and powdery bacterium all are comparatively ideal novel form microbial inoculums.
Describe the present invention in detail below in conjunction with embodiment.
Used material, instrument, equipment and source are as follows: 1, its representative species of unsaturated double-bond unimolecule organic matter is selected acrylamide AM for use, the production of Dalian chemical reagent factory, technical grade, content 30%; 2, crosslinking agent is selected N, N '-methene base bisacrylamide chemical pure, Beijing Chemical Plant for use; 3, initator, tetramethyl second 2 amine; 4, Ammonium Persulfate 98.5 is the chemical pure Beijing Chemical Plant and produces.5, bacterial classification, white muscardine fungi 9# is for Jilin Academy of Agricultural Sciences produces, slant strains.6, RJS polymeric gel, Tech. Physics Inst., Sci. Academy of Heilongjiang Prov. provides.7, nutrients is selected corn starch and wheat bran skin, Harbin grain depot for use.8, JZ7114 type plant disintegrating machine.9, ZAX2 type biological photography microscope, Shanghai camera three factories; 10, liquid nutrient medium selects for use PDA potato liquid nutrient medium to contain sucrose 2%PH nature.11, bacterium determination of activity, micro-mensuration counting, the field practices.
Polymeric gel biological bacteria pesticide is by biological bacteria and spore not and two key unimolecule organic matters, crosslinking agent, initator, stabilizing agent and nutrients mixed preparing, can make pearl desinsection microbial inoculum, change technology a little and can make the powdery agrichemical microbial inoculum, described Biocidal bacterium is selected white muscardine fungi and Bactospeine for use, also is fit to the other biological bacterial parasite.
Embodiment 1: the polymeric gel biological bacteria pesticide microbial inoculum be by: by active bacterium powder 30 its spore contents of gram of the contained weight of 500ml unit volume is 10 * 10
11Individual/gram, 20% acrylamide AM500ml mixes mutually and adds 3%N, N '-methene base bisacrylamide crosslinker simultaneously together, 0.4% tetramethyl second, 2 amine initators, 0.1% ammonium persulfate stabilizing agent, 120 gram corn starchs and 30 gram wheat bran nutrients are mixed and make polymeric gel biological bacteria pesticide.Its preparation technology is as follows:
Polymeric gel biological bacteria pesticide pearl fungicide preparation technology, it is by the contained weight of 500ml unit volume active bio bacterium bacterium powder 30 grams to be arranged, spore content is 10 * 10
11Individual/gram mixes mutually with the acrylamide AM500ml of concentration 20% and adds 3%N, N '-methene base bisacrylamide crosslinker simultaneously, 0.4% tetramethylethylenediamine initator, 0.1% ammonium persulfate stabilizing agent, 120 gram corn starchs and 30 gram wheat bran nutrients mixed 30 minutes down at 43 ℃, become the gel biological bacteria mixture, place former to be molded into the pearl particle again, after 35 ℃ of dryings, get finished product 160 grams to 180 grams.Can be when producing in batches by combinations thereof content proportioning.The pearl microbial inoculum forms and mainly is subjected to initator, monomer concentration influence, and it is that index is tested that mycelia is inhaled splash multiple and sporulation quantity, monomer concentration is a principal element, secondly is initiator amount.The present invention is optimized combinatorial test and obtains a result monomer concentration 20%, and initiator amount 0.4% crosslinking agent 3% ± 0.1% nutrient inventory is 150 grams ± 2 grams.
Embodiment 2: powdery polymeric gel biological bacteria pesticide and preparation technology thereof.Get 1 test tube white muscardine fungi 9# original seed and insert in the PDA potato liquid nutrient medium, from 50ml, 250ml to 500ml step by step amplification culture be three grades of liquid bacterium.The same 1%N of 20% acrylamide AM500ml, N '-methene base acrylamide is mixed mutually with 0.4% tetramethylethylenediamine and 0.1% ammonium persulfate, the insulation polymerization is into about the 500ml polymeric gel in 41 ℃, claim RJS glue, cut into 30 order fragments, amount to 4000 gram nutrients with 1000 gram wheat brans and 3000 gram corn flour and mix mutually and sterilize.The cooling back adds three grades of bacterium of above-mentioned 500ml liquid and makes the gel bacterium in fermentation in 26 ℃ of following 7-10 days, cross 40 mesh sieves in 35 ℃ of dry backs, can get finished product 4200-4500 gram, batch process can be with reference to said ratio, three grades of bacterium of liquid can be used the artificial synthetic medium: contain corn flour 5%, wheat bran 1.5%, bean powder 0.5%, infusion 15-20 minute, get final product after the filtration.
Pearl microbial inoculum and powdery microbial inoculum compare: form from carrier, it is carrier that the powdery microbial inoculum mainly contains with the high suction of RJS multiple gel, and pearl bacterium glue is that special process once is polymerized.Seeing in nature of bacterium powder, powdery microbial inoculum are to contain a small amount of spore based on mycelia, and the pearl microbial inoculum almost is the bacterium oidium.The pearl microbial inoculum has the anti-uv-ray stronger than the powdery microbial inoculum, but the pearl microbial inoculum easily comes off from blade during insect on the ground in control, and the powdery microbial inoculum easily forms a large amount of zoogloeas at blade base, has planting the character of adhering on the pearl and can spray by dry powder.
Claims (2)
1, a kind of polymeric gel biological bacteria pesticide is characterized in that being restrained, being mixed mutually with the unimolecule organic matter 20% concentration 500ml that contains unsaturated double-bond and an amount of crosslinking agent, initator, stabilizing agent and 150-160 gram starch nutrition thing by activated biological bacteria 20-30 by the contained weight of 500ml unit volume.
2, a kind of polymeric gel biological bacteria pesticide preparation technology, it is characterized in that to mix 40-45 ℃ of insulation with 150-160 gram starch nutrition thing with unimolecule organic matter 20% concentration 500ml that contains unsaturated double-bond and an amount of initator, crosslinking agent, stabilizing agent by the active bio bacterium 20-30 gram of the contained weight of 500ml unit volume and became polymeric gel biological bacteria in 30 minutes, through former beading particle, make the pearl polymerisation gel biological bacteria pesticide again 35 ℃ of following dryings.
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| Application Number | Priority Date | Filing Date | Title |
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| CN94103705A CN1059780C (en) | 1994-03-31 | 1994-03-31 | Polymeric gel biological bacteria pesticide and preparation process thereof |
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| CN94103705A CN1059780C (en) | 1994-03-31 | 1994-03-31 | Polymeric gel biological bacteria pesticide and preparation process thereof |
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| CN1093524A true CN1093524A (en) | 1994-10-19 |
| CN1059780C CN1059780C (en) | 2000-12-27 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011113061A3 (en) * | 2010-03-12 | 2013-01-10 | Monsanto Technology Llc | Agrochemical gel compositions |
| CN106316659A (en) * | 2016-07-28 | 2017-01-11 | 安徽尚善生物科技股份有限公司 | Insect-resistant fertilizer filler with purple cabbage waste residues as main material and preparation method thereof |
| CN112825865A (en) * | 2020-12-31 | 2021-05-25 | 吉林省农业科学院 | Insecticidal composition, application thereof and insecticide |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1058130A (en) * | 1990-07-20 | 1992-01-29 | 王缉健 | Releaser for beauveria bassiana spore powder |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011113061A3 (en) * | 2010-03-12 | 2013-01-10 | Monsanto Technology Llc | Agrochemical gel compositions |
| AU2011226523B2 (en) * | 2010-03-12 | 2015-02-26 | Monsanto Technology Llc | Agrochemical gel compositions |
| US11419329B2 (en) | 2010-03-12 | 2022-08-23 | Monsanto Technology Llc | Agrochemical gel compositions |
| CN106316659A (en) * | 2016-07-28 | 2017-01-11 | 安徽尚善生物科技股份有限公司 | Insect-resistant fertilizer filler with purple cabbage waste residues as main material and preparation method thereof |
| CN112825865A (en) * | 2020-12-31 | 2021-05-25 | 吉林省农业科学院 | Insecticidal composition, application thereof and insecticide |
| CN112825865B (en) * | 2020-12-31 | 2022-07-12 | 吉林省农业科学院 | Insecticidal composition, application thereof and insecticide |
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