CN1237346A - Bacillus subtilis preparation, production method and its application - Google Patents
Bacillus subtilis preparation, production method and its application Download PDFInfo
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- CN1237346A CN1237346A CN 99114260 CN99114260A CN1237346A CN 1237346 A CN1237346 A CN 1237346A CN 99114260 CN99114260 CN 99114260 CN 99114260 A CN99114260 A CN 99114260A CN 1237346 A CN1237346 A CN 1237346A
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- bacillus subtilis
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Abstract
The present invention relates to a bacillus subtilis preparation, its production and application method, belonging to the field of microbial fermentation and biological control of plant diseases. Said preparation contains Caifengning B1 strain, Caifengning B2 strain and Maifengning B3 strain, and the total quantity of the strains is above five hundred million per gm. Its production method includes the following steps: activation of inoculum, seeding tank culture, amplification culture and preparing powder, and its application adopts the methods of seed dressing, irrigating root, dipping root and spraying, and the dosage of said preparation for per mu is 100-300 g. Said invented product is simple in use, pollutionless, safe for human and animal, and has no side effect.
Description
Bacillus subtilis microbial agent of the present invention, method for making and using method thereof belong to microbial fermentation and biocontrol of plant disease field, are exclusively used in the biological control of plant disease.
Along with the drawback (poison people and animals, destroy the ecological balance etc.) of chemical pesticide is familiar with by people gradually, biological control comes into one's own day by day.In the plant pest biological control, the most successful with insect biological and ecological methods to prevent plant disease, pests, and erosion especially Bt biopesticide control lepidoptera pest, the disease biological and ecological methods to prevent plant disease, pests, and erosion is comparatively backward, the main disease-preventing microbial that utilizes has that bacillus, wood are mould, actinomycetes etc., wherein active with the research and development of bacillus, abroad oneself is successfully used to fruit anti-corrosive fresh-keeping etc., the domestic research of carrying out preventing and treating wheat scab, bacterial blight of rice biocontrol fungicide, but be limited to indoor plate screening more, seldom move towards the land for growing field crops, as yet not large tracts of land field trial and suitability for industrialized production.
The purpose of this invention is to provide a kind of bacillus subtilis microbial agent, method for making and using method thereof, be used for the biological control of plant disease, have preventing disease production-increasing effect, be fit to suitability for industrialized production, product uses simple, and is pollution-free, have no side effect, and has the characteristics of biopesticide to the person poultry safety.
It is more than 500,000,000/gram that bacillus subtilis microbial agent provided by the present invention contains dish Fengning B1 bacterial strain, dish Fengning B2 bacterial strain, wheat Fengning B3 bacterial strain total amount.
The bacillus subtilis microbial agent method for making comprises:
1 fermentation:
(1) actication of culture
(2) seed tank culture: fermentative medium formula is soybean cake powder 1-3%, wheat bran 1-3%, CaCO
30.1%, MgSO
40.03%, (NH
4)
2SO
40.03%, Na
2HPO
40.2%.
After the bacterial classification enlarged culture after the activation, insert 1 ton of seeding tank, the inoculation bacteria concentration is 10
9-10
11CFU/ml, the inoculation volume is the 100-200 milliliter.The seed tank culture temperature is 30 ℃-32 ℃, and throughput is 1: 1, and mixing speed is 300 rev/mins.The seed tank culture time is 25-40 hour.Carrying out quality and bacterium amount between culture period detects.
(3) amplification culture: fermentation time is 36 hours, the same seeding tank of culture medium prescription, fermentation condition and quality determining method.
2. pulvis is made: zymotic fluid and adsorbent are pulverized by 1: 10 mixing, and 150 orders sieve.
The bacillus subtilis strain that uses in the bacillus subtilis microbial agent is as dish Fengning B1 bacterial strain, dish Fengning B2 bacterial strain, wheat Fengning B3 bacterial strain or be the mixture with any ratio of these three kinds of bacterial strains.Microbial inoculum is liquor and pulvis.
The using method of above-mentioned bacillus subtilis microbial agent is, every mu with microbial inoculum 100-300 gram, adds water 40-60 kilogram, adopts seed dressing, irritates root, dips in root, method controlling plant diseases such as spraying.The using method of Tui Chonging is to handle in seed treatment or seedling stage the most.
The bacillus subtilis strain that uses in the bacillus subtilis microbial agent is public bacterial strain as dish Fengning B1 bacterial strain, dish Fengning B2 bacterial strain, wheat Fengning B3 bacterial strain, it measures authentication method is known technology, document " Agricultural University Of Nanjing's journal " 1994 sees reference, 17 (supplementary issues), 63-66, " classification of 8 strain disease flocking biocontrol Bacillus strains is identified ".
Bacillus subtilis microbial agent provided by the present invention and using method thereof are fit to suitability for industrialized production, and product uses simple, and be pollution-free, have no side effect, and has the advantage of biopesticide to the person poultry safety.Be used for the biological control of plant disease, especially the field control effect of bacterial disease reaches more than the 60-80%, and the vegetative stage (as seed sprouting, growth of seedling, reproductive growth etc.) of trial crops is had obvious facilitation, more than the volume increase 10-30%.Wherein, be 70% to the control efficiency of soft rot of Chinese cabbage, surpass agricultural streptomycin, volume increase Chinese cabbage 20%; Control sclerotinia rot of colza effect is more than 60%, and is close with carbendazim, volume increase rape seed 15%; The effect of control wheat sharp eyespot is 60%, is better than jinggangmeisu and triadimefon, and the volume increase wheat is more than 10%.Product is workable, is easily accepted by technical cadre and peasant, is easy to grasp.Bacillus subtilis microbial agent large tracts of land provided by the present invention use the result prove its input-output ratio minimum be 1: 8, be generally about 1: 15.
The evidence bacillus subtilis microbial agent contains bacillus subtilis, antibiotic and short living beings, and its disease-preventing and yield-increasing mechanism is: 1. suppress pathogen growth breeding, interrupt germ history of life; 2. induce crop that germ is produced resistance; 3. change the microbiologic population that encloses as object; 4. produce growth regulatory substance; 5. the plant active component is grown effect surely.
With " dish Fengning B1 bacterial strain " is that the microbial inoculum of Main Ingredients and Appearance is used to prevent and treat soft rot of Chinese cabbage, and control efficiency is 70%, volume increase Chinese cabbage 20%; With " dish Fengning B2 bacterial strain " is that the microbial inoculum of Main Ingredients and Appearance is used to prevent and treat sclerotinia rot of colza, and effect is more than 60%, volume increase rape seed 15%; With " wheat Fengning B3 bacterial strain " is that the effect that the microbial inoculum of Main Ingredients and Appearance is used to prevent and treat wheat sharp eyespot is 60%, and the volume increase wheat is more than 10%.Above-mentioned three kinds of products have all passed through pilot scale, are successively produced by Nanjing letter major company Jiangpu pilot plant, Nanjing biopesticide factory and Huaiyin Electrochemical Plant.B1 and B2 fermentation index reach 1 * 10
10, the B3 fermentation index reaches 4 * 10
9More than, product meets the pulvis standard, and bacteria containing amount all reaches more than 500,000,000/g, factory's input-output ratio 1: 3, three kinds of biopesticides accumulative totals are used 5,500,000 mu of areas, and product is nuisanceless, and is pollution-free, welcome by society, produced huge social economy and ecological benefits.
Embodiment 1:
It is more than 500,000,000/gram that bacillus subtilis microbial agent contains dish Fengning B1 bacterial strain total amount.Method for making comprises:
1 fermentation
1) dish Fengning B1 bacterial strain actication of culture
2) seed tank culture: fermentative medium formula is a soybean cake powder 1%, wheat bran 1%, CaCO
30.1%, MgSO
40.03%, (NH
4)
2SO
40.03%, Na
2HPO
40.2%.
After the bacterial classification enlarged culture after the activation, insert 1 ton of seeding tank, the inoculation bacteria concentration is 10
9CFU/ml, the inoculation volume is 100 milliliters.The seed tank culture temperature is 30 ℃, and throughput is 1: 1, and mixing speed is 300 rev/mins.The seed tank culture time is 25 hours.Carrying out quality and bacterium amount between culture period detects.
3) amplification culture: fermentation time is 36 hours, the same seeding tank of culture medium prescription, fermentation condition and quality determining method.
2. pulvis is made: zymotic fluid and adsorbent are pulverized by 1: 10 mixing, and 150 orders sieve.
Above-mentioned is that the microbial inoculum of Main Ingredients and Appearance is used to prevent and treat soft rot of Chinese cabbage with " dish Fengning B1 bacterial strain ", and the using method of bacillus subtilis microbial agent is, every mu restrains with microbial inoculum 100-300, add water 40-60 kilogram, adopt seed dressing, irritate methods such as root, spraying, control efficiency is 70%, volume increase Chinese cabbage 20%.Embodiment 2:
It is more than 500,000,000/gram that bacillus subtilis microbial agent contains dish Fengning B2 bacterial strain amount.The microbial inoculum method for making comprises:
1 fermentation
1) dish Fengning B2 bacterial strain actication of culture;
2) seed tank culture: fermentative medium formula is a soybean cake powder 3%, wheat bran 3%, CaCO
30.1%, MgSO
40.03%, (NH
4)
2SO
40.03%, Na
2HPO
40.2%.
After the bacterial classification enlarged culture after the activation, insert 1 ton of seeding tank, the inoculation bacteria concentration is 10
11CFU/ml, the inoculation volume is 200 milliliters.The seed tank culture temperature is 32 ℃, and throughput is 1: 1, and mixing speed is 300 rev/mins.The seed tank culture time is 40 hours.Carrying out quality and bacterium amount between culture period detects.
3) amplification culture: fermentation time is 36 hours, the same seeding tank of fermentation condition and quality determining method.
2. pulvis is made: zymotic fluid and adsorbent are pulverized by 1: 10 mixing, and 150 orders sieve.
Be used to prevent and treat sclerotinia rot of colza with bacillus subtilis dish Fengning B2 bacterial strain microbial inoculum liquor or pulvis, every mu with microbial inoculum 100 gram, adds water 40-60 kilogram, adopts seed dressing, dips in method controlling plant diseases such as root, spraying.The using method of Tui Chonging is to handle in seed treatment or seedling stage the most.Effect is more than 60%, volume increase rape seed 15%: embodiment 3:
Bacillus subtilis microbial agent wheat Fengning B3 bacterial strain amount is more than 500,000,000/gram.The microbial inoculum method for making comprises:
1 fermentation:
1) wheat Fengning B3 bacterial strain actication of culture
2) seed tank culture: fermentative medium formula is a soybean cake powder 2%, wheat bran 2%, CaCO
30.1%, MgSO
40.03%, (NH
4)
2SO
40.03%, Na
2HPO
40.2%.
After the bacterial classification enlarged culture after the activation, insert 1 ton of seeding tank, the inoculation bacteria concentration is 10
10CFU/ml, the inoculation volume is 100 milliliters.The seed tank culture temperature is 30 ℃, and throughput is 1: 1, and mixing speed is 300 rev/mins.The seed tank culture time is 30 hours.Carrying out quality and bacterium amount between culture period detects.
3) amplification culture: fermentation time is 36 hours, the same seeding tank of culture medium prescription, fermentation condition and quality determining method.
2. pulvis is made: zymotic fluid and adsorbent are pulverized by 1: 10 mixing, and 150 orders sieve.
Above-mentioned bacillus subtilis wheat Fengning B3 bacterial strain microbial inoculum is liquor or pulvis, and every mu with microbial inoculum 100 gram, adds water 40-60 kilogram, and adopting the effect of methods control wheat sharp eyespots such as seed dressing, spraying is 60%, increases production wheat more than 10%.
No matter with the Fengning B1 bacterial strain of serving, dish Fengning B2 bacterial strain, three kinds of microbial inoculums of wheat Fengning B3 bacterial strain is that liquor or pulvis all can any ratio mix use, and this is that those of ordinary skill can be accomplished, and effect is also fine.The actication of culture program also is that those of ordinary skill is familiar with and uses always in the technology.
Claims (5)
1. bacillus subtilis microbial agent is characterized in that: containing dish Fengning B1 bacterial strain, dish Fengning B2 bacterial strain, wheat Fengning B3 bacterial strain total amount is more than 500,000,000/gram.
2. the described bacillus subtilis microbial agent method for making of claim 1 comprises:
1) actication of culture;
2) seed tank culture: after the bacterial classification enlarged culture after the activation, insert 1 ton of seeding tank, the inoculation bacteria concentration is 10
9-10
11CFU/ml, the inoculation volume is the 100-200 milliliter, and the seed tank culture temperature is 30 ℃-32 ℃, and throughput is 1: 1, and mixing speed is 300 rev/mins, and the seed tank culture time is 25-40 hour, and zymotic fluid is microbial inoculum;
3) amplification culture: fermentative medium formula is soybean cake powder 1-3%, wheat bran 1-3%, CaCO
30.1%, MgSO
40.03%, (NH
4)
2SO
40.03%, Na
2HPO
40.2%, the same seeding tank of fermentation condition.
3. according to the described bacillus subtilis microbial agent method for making of claim 2, it is characterized in that, increase pulvis and make: zymotic fluid and adsorbent are pulverized by 1: 10 mixing, and 150 orders sieve.
4. the using method of the described bacillus subtilis microbial agent of claim 1 comprises: every mu with microbial inoculum 100-300 gram, adds water 40-60 kilogram, adopts seed dressing, irritates root, dips in root, method controlling plant diseases such as spraying.
5. according to the using method of the described bacillus subtilis microbial agent of claim 4, best using method is to handle in seed treatment or seedling stage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99114260 CN1237346A (en) | 1999-06-15 | 1999-06-15 | Bacillus subtilis preparation, production method and its application |
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|---|---|---|---|
| CN 99114260 CN1237346A (en) | 1999-06-15 | 1999-06-15 | Bacillus subtilis preparation, production method and its application |
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| CN1237346A true CN1237346A (en) | 1999-12-08 |
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ID=5277366
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| CN 99114260 Pending CN1237346A (en) | 1999-06-15 | 1999-06-15 | Bacillus subtilis preparation, production method and its application |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101861877A (en) * | 2010-06-18 | 2010-10-20 | 河南省农业科学院 | Seed treating agent |
| CN101993836A (en) * | 2010-06-13 | 2011-03-30 | 河南省农业科学院 | Bacillus subtilis strain YB-81, fungicide and preparation method and application thereof |
| CN102391965A (en) * | 2011-11-17 | 2012-03-28 | 吉林省集韩生物肥有限公司 | Preparation method of biological agent for preventing and treating ginseng diseases |
| CN102757277A (en) * | 2012-07-31 | 2012-10-31 | 长春华御实业集团有限公司 | Paddy biofertilizer, preparation method thereof and bacillus subtilis |
| CN102871008A (en) * | 2012-10-11 | 2013-01-16 | 北京大北农科技集团股份有限公司 | Simple preparation method of bacillus bacteria powder |
| CN105255773A (en) * | 2015-11-05 | 2016-01-20 | 王志民 | Use method of plant space-occupying bacteria |
| CN107760641A (en) * | 2017-12-11 | 2018-03-06 | 天津市农业生物技术研究中心 | A kind of bacillus subtilis microbial agent and its application |
| CN111296208A (en) * | 2020-03-25 | 2020-06-19 | 山西省农业科学院作物科学研究所 | Scutellaria baicalensis seedling growing method |
| CN112136583A (en) * | 2020-09-17 | 2020-12-29 | 保定市科绿丰生化科技有限公司 | Bacillus subtilis NCD-2-based banana wilt prevention and control method and application |
-
1999
- 1999-06-15 CN CN 99114260 patent/CN1237346A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993836A (en) * | 2010-06-13 | 2011-03-30 | 河南省农业科学院 | Bacillus subtilis strain YB-81, fungicide and preparation method and application thereof |
| CN101861877A (en) * | 2010-06-18 | 2010-10-20 | 河南省农业科学院 | Seed treating agent |
| CN102391965A (en) * | 2011-11-17 | 2012-03-28 | 吉林省集韩生物肥有限公司 | Preparation method of biological agent for preventing and treating ginseng diseases |
| CN102391965B (en) * | 2011-11-17 | 2013-01-30 | 吉林省集韩生物肥有限公司 | Preparation method of biological agent for preventing and treating ginseng diseases |
| CN102757277A (en) * | 2012-07-31 | 2012-10-31 | 长春华御实业集团有限公司 | Paddy biofertilizer, preparation method thereof and bacillus subtilis |
| CN102871008A (en) * | 2012-10-11 | 2013-01-16 | 北京大北农科技集团股份有限公司 | Simple preparation method of bacillus bacteria powder |
| CN105255773A (en) * | 2015-11-05 | 2016-01-20 | 王志民 | Use method of plant space-occupying bacteria |
| CN107760641A (en) * | 2017-12-11 | 2018-03-06 | 天津市农业生物技术研究中心 | A kind of bacillus subtilis microbial agent and its application |
| CN111296208A (en) * | 2020-03-25 | 2020-06-19 | 山西省农业科学院作物科学研究所 | Scutellaria baicalensis seedling growing method |
| CN112136583A (en) * | 2020-09-17 | 2020-12-29 | 保定市科绿丰生化科技有限公司 | Bacillus subtilis NCD-2-based banana wilt prevention and control method and application |
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