CN115644187A

CN115644187A - Butterflybush and metarhizium anisopliae composite microbial inoculum and application thereof

Info

Publication number: CN115644187A
Application number: CN202211223775.6A
Authority: CN
Inventors: 涂雄兵; 潘凡; 王芳; 朱凯辉; 张泽华; 张蓉
Original assignee: Institute of Plant Protection of Chinese Academy of Agricultural Sciences; Institute of Plant Protection of Ningxia Academy of Agriculture and Forestry Sicience
Current assignee: Institute of Plant Protection of Chinese Academy of Agricultural Sciences; Institute of Plant Protection of Ningxia Academy of Agriculture and Forestry Sicience
Priority date: 2022-10-09
Filing date: 2022-10-09
Publication date: 2023-01-31

Abstract

The invention discloses a butyl and green muscardine fungus composite microbial inoculum and application thereof. The butyl cloth and the metarhizium anisopliae are used in a combined mode, the aphids are fed in a feeding mode, the growth and the propagation of the aphids can be obviously inhibited, the butyl cloth and the metarhizium anisopliae are used in a combined synergistic mode, the using amount of chemical pesticides can be reduced, the pest control efficiency is improved, and resistance is not easy to generate.

Description

Butterflybush and metarhizium anisopliae composite microbial inoculum and application thereof

Technical Field

The invention belongs to the technical field of biological pesticides, and particularly relates to a butylbenzene and metarhizium anisopliae composite microbial inoculum and application thereof.

Background

Aphis gossypii belongs to an important pest of medlar, and damages young leaves, flower buds, young fruits and growing points of medlar through piercing and sucking, thereby causing the yield loss of medlar. The cotton aphid has large occurrence area and serious harm in Chinese wolfberry main production areas. At present, the chemical prevention and control of cotton aphids are still mainly carried out, and chemical pesticides are indiscriminately used and abused, so that the drug resistance of the cotton aphids is enhanced, the pesticide residue of the medlar is increased, and the health care and medicinal value of the cotton aphids is reduced. In order to ensure the healthy development of the medlar industry, the passive situation that the medlar pests are mainly prevented and controlled by chemical pesticides at the present stage is changed, and the development of novel and efficient green prevention and control products for dealing with the harm of medlar cotton aphids is urgently needed.

The Metarhizium anisopliae is used as a biological pesticide with wide application, has the advantages of wide host range, no toxicity to human, livestock and non-target organisms, no residue, long lasting period and the like, and has wide application prospect. However, the single use of fungus biopesticides such as metarhizium anisopliae for pest control has the characteristics of slow pest killing speed, low pest killing effect and the like, so that the search for a compound which is used together with the fungus and has synergistic property is very important.

Hydroxamic acid (Hydroxamic acid) and derivatives thereof are compounds with anti-pest allelopathy effect, and the substances are represented by butylbenzene and are important compounds for researching the interrelation of insects and plants. The study finds that the ambu inhibits the growth and development of insects, has effects on the development period, the reproduction and the like, and can inhibit the growth and development of insects by destroying the activity of various detoxification enzymes in the insects. But the research on the insecticidal effect of the composition on the cotton aphid of the medlar and the synergistic effect of the composition on the fungal insecticide is less. Therefore, the study on the effect of the composition on the cotton aphid with medlar in combination with the metarhizium anisopliae is very important for improving the problem of slow insecticidal effect of the metarhizium anisopliae.

Disclosure of Invention

The invention aims to provide a pudding and metarhizium anisopliae composite microbial inoculum and application thereof.

A bulrush and metarhizium anisopliae compound microbial inoculum comprises active components of bulrush and metarhizium anisopliae.

The dosage ratio of the butyl fabric to the metarhizium anisopliae is (400-600) mu g:10 ⁸ And (4) spores.

The complex microbial inoculum also comprises a pharmaceutically acceptable carrier and water.

The pharmaceutically acceptable carrier is one or a combination of more of agricultural milk 500, agricultural milk 600, agricultural milk 1602, diatomite and attapulgite.

The use of the compound fungicide of butyl and green muscardine fungus in preventing and controlling cotton aphid.

The invention has the beneficial effects that: the butyl cloth and the metarhizium anisopliae are used in a combined mode, the aphids are fed in a feeding mode, the growth and the propagation of the aphids can be obviously inhibited, the butyl cloth and the metarhizium anisopliae are used in a combined synergistic mode, the using amount of chemical pesticides can be reduced, the pest control efficiency is improved, and resistance is not easy to generate.

Drawings

FIG. 1 shows the aphid mortality for different treatments.

FIG. 2 shows the aphid reproduction rate under different treatments.

Detailed Description

In order that the invention may be more fully understood, reference will now be made to the following description. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

Metarhizium anisopliae used in the following examples was deposited at the institute for plant protection, national academy of agricultural sciences, code IPPIMI330189.

Example 1

A test insect source: the test insects are bred in an artificial climate incubator. The breeding conditions are that the temperature is 26 ℃, the humidity is 75%, and the illumination time is as follows: dark time =16h: and 8h.

Test strains: inoculating Metarrhizium anisopliae on PDAY culture medium, culturing at 25 deg.C for 12d, scraping off conidia, drying, and storing at 4 deg.C.

The determination was carried out by a double-layered Parafilm M sandwich liquid artificial feed feeding method. 30 healthy adult aphids which breed several generations are selected in an artificial climate incubator and put into an aphid incubator, and each treatment is repeated for 5 times. The aphids are fed without respectively mixing the butyl cloths with different concentrations into 200 mul aphid feed (table 1), blank artificial feed is used as a control, the test period is one week, the test conditions are that the temperature is 26 ℃, the humidity is 75%, and the illumination time is as follows: dark time =16h: at 8h, aphid mortality (FIG. 1) and reproductive rate (FIG. 2) were observed and recorded.

TABLE 1 different treatment Components and concentrations

As can be seen from the analysis of figure 2, the single treatment of the ambrette at the concentrations of 600 mug/ml and 800 mug/ml has lower multiplication rate of cotton aphid than the single treatment of other concentrations and green muscardine fungus. The reproduction rate of cotton aphids treated by the butyl cloths with various concentrations and the green muscardine fungi together is lower than that of the cotton aphids treated by the green muscardine fungi alone, wherein the reproduction rate of the cotton aphids treated by the butyl cloths with 400 mu g/ml, 600 mu g/ml and 800 mu g/ml and the green muscardine fungi together is obviously lower than that of the cotton aphids treated by the green muscardine fungi alone. The lowest reproduction rate of the strain treated by the strain and the metarhizium anisopliae under the concentration of 600 mu g/ml is 41.33 percent.

Therefore, the lethal effect and the growth inhibition effect are comprehensively considered, the metarhizium anisopliae and the 600ug/ml butyl cloth can be selected to be mixed and applied, the use pollution of chemical pesticides can be reduced to a great extent, and the problem of long lethal time of fungi on pests by single application can be solved while the drug resistance of the pests is slowed down.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that various changes and modifications can be made by those skilled in the art without departing from the spirit of the invention, and these changes and modifications are all within the scope of the invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. The ambu and metarhizium anisopliae compound microbial inoculum is characterized in that the active components of the compound microbial inoculum are ambu and metarhizium anisopliae.

2. The ambu-metarhizium anisopliae composite microbial inoculum according to claim 1, wherein the dose ratio of the ambu to the metarhizium anisopliae is (400-600) μ g:10 ⁸ And (4) spores.

3. The bubba and metarhizium anisopliae complex microbial inoculum according to claim 1, which further comprises a pharmaceutically acceptable carrier and water.

4. The bulrush and metarhizium anisopliae composite microbial inoculum according to claim 1, wherein the pharmaceutically acceptable carrier is one or a combination of agricultural milk 500, agricultural milk 600, agricultural milk 1602, diatomite and attapulgite.

5. The butyl cloth and green muscardine fungus compound bacteria agent is applied to preventing and controlling cotton aphids.