CN1554242A - Trichoderma fungus biological control granular bacterial agent and its preparing method - Google Patents
Trichoderma fungus biological control granular bacterial agent and its preparing method Download PDFInfo
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- CN1554242A CN1554242A CNA2003101241219A CN200310124121A CN1554242A CN 1554242 A CN1554242 A CN 1554242A CN A2003101241219 A CNA2003101241219 A CN A2003101241219A CN 200310124121 A CN200310124121 A CN 200310124121A CN 1554242 A CN1554242 A CN 1554242A
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Abstract
The bacteria preparation consists of pure live trichoderma fungus, granular solid culture medium and coating agent; and is prepared through fungus seed separation, purifying and culture, preparing granular solid culture medium, inoculation and fermentation, coating with the coating agent, air drying and other steps. The granular bacteria preparation has guarantee period as long as 2 years, convenient use, fast proliferation speed of antagonizing bacteria, fast effect and excellent biological control effect on rhizoctonia disease, blight, etc of grain crop, cotton, vegetable, etc.
Description
Technical field
The present invention relates to microbial pesticide and preparation method thereof, especially relate to a kind of fungus Trichoderma biological control granular bacteria preparation and preparation method thereof.
Background technology
Long-term next, because the residue problem that unreasonable use chemical pesticide is produced, brought well-known environmental pollution, the ecological balance is destroyed and the uneasy congruent a series of problems of food, human and livestock health and life security have been produced harm, therefore, research and development utilize beneficial microbe pesticide control diseases and pests of agronomic crop to cause people's great attention.Wherein, the microbial activity microbial inoculum is one of main contents of microbial pesticide.That application is maximum in the current domestic and international fungus Trichoderma is trichoderma harzianum (Trichoderma harzianum), and common formulations is the wheat bran preparation.The wheat bran preparation of U.S.'s Trichoderma harzianum is used under field condition, can reduce cotton seedling blight 60%, and improves emergence rate 14%; (Pineda-JB, 2001; Nagaraju-P, 2002; Gracia-Garza-JA, 2003 or the like) also there is like product listing (agriculture chemical registration number: LS94694) in Yuhuan county, domestic Zhejiang Province.But present wooden mould wheat bran preparation, mainly form with the generation spore and the wheat bran of fungus Trichoderma, be powdery, its weak point is that the shelf-life is shorter, the spore survival period of border mensuration product had only about half a year factually, spore is relatively poor in field sprouting and adaptability simultaneously, and ecotope is had relatively high expectations; Therefore, from growing propagation surely, it is slower to begin to bring into play the speed that suppresses plant disease after forming dominant microflora, and in addition, the wheat bran preparation is subject to production environment such as influences such as strong wind, rainwater in the field, also reduced effect.
Summary of the invention
The present invention seeks to the weak point at fungus Trichoderma wheat bran preparation, develop a kind of long shelf-life, it is convenient to use, it is fast that growth rate is grown in the Trichoderma growth surely, it is little influenced by production environment, and protection effect is good, lower-cost granular pattern microbial inoculum and supporting preparation method thereof.
The objective of the invention is to be achieved by the following technical programs.
Fungus Trichoderma biological and ecological methods to prevent plant disease, pests, and erosion granular bacteria preparation is made up of fungus Trichoderma pure culture viable bacteria and granular solids medium thing and seed coating medicine;
Described fungus Trichoderma bacterial classification is Trichoderma viride (Trichoderma vivid) or this bacterial classification and Trichoderma harzianum (Trichoderma harzianum), yellow green trichoderma (Trichoderma aureoviride), tangerine green trichoderma (Trichoderma citrinouiride) (" Chinese fungi aggregation " Dai Fanglan, Science Press, 1997, " fungi identification handbook " Wei Jingchao, Shanghai science and technology is published, 1979) among any one or some kinds press the compound fungi strain of arbitrary proportion.
Described granular solids medium thing is the particle diameter particle of choosing any one kind of them among the millet between the 1-5mm, rice, corn, barley and wheat or jowar or is the formulated the same granular formulations of particle diameter of raw material and other auxiliary material with above-mentioned basic thing powder.
Described seed coating medicine is mixed by coating powder and binding agent, and coating powder is the raw material of choosing any one kind of them among the above-mentioned basic thing, the ultra micro powder that processes, and wherein the ultra micro powder of wheat or rice is a preferred feedstock; Binding agent is the mixture of sodium carboxymethylcellulose (CMC) or sodium carboxymethyl starch and water.
The preparation method of described fungus Trichoderma biological and ecological methods to prevent plant disease, pests, and erosion granular bacteria preparation, it is prepared from through following machining process:
(1) separation of fungus Trichoderma bacterial classification, purifying and cultivation reach 100% purity and do not have other assorted bacterium to bacteria containing amount;
(2) preparation of granular solids medium thing: will select the particle of basic thing or be the granular formulations that raw material and other auxiliary material are mixed with this base thing powder, therefrom filter out the particle of particle diameter at 1-5mm, at 15-20 pound pressure, 120 ℃ of left and right sides high pressure steam sterilizations cooled off under the room temperature after 30 minutes;
(3) bacterial classification inoculation of granular solids medium thing and fermented and cultured: after the equipment disinfection sterilization, under aseptic condition, bacterial classification and granular solids medium thing are inoculated by 1: 200～300 part by weight at normal temperatures, 20～30 ℃ of room temperatures, under humidity 65～90% conditions, fermented and cultured 4～7 days, during the studded shape solid culture medium of Trichoderma thing surface;
(4) preparation of seed coating medicine: (a) with above-mentioned sodium carboxymethylcellulose (CMC) or sodium carboxymethyl starch and water in 1～3 (gram weight): 1000 (milliliter volume) ratio is mixed mutually, stir into scattered paste shape binding agent; (b) above-mentioned optional basic thing is crossed 250～360 mesh sieves after attrition process and be ultra micro shape coating powder; (c) with coating powder and scattered paste shape binding agent in 50～100 (gram weight): 1～10 (milliliter volume) ratio is mixed mutually, makes seed coating medicine through stirring.
(5) the carry disease germs dressing of granular solids medium thing, dry and packing: the granular solids medium thing that will connect behind the strain fermentation is poured in an amount of seed coating medicine, pull out after stirring and spread out, with the air-dry back of normal temperature air-flow pack product.
Beneficial effect of the present invention, the one, granular bacteria preparation contains the graininess core, and it is the strain fermentation medium, is again the carrier of mycelium and spore, and the coating protection is arranged again outside it, storage environment is had stronger adaptive capacity, so its shelf-life was about 2 years; Two are to use conveniently, are not subject to production environment as blowing, and weather condition influence such as rain is wider to production environment accommodation, but generally at 10～40 ℃ of equal growth and breedings; The 3rd, after used in the field, bacterial classification was grown surely, reproduction speed is fast, because of still having a certain amount of remaining medium thing in this microbial inoculum, and mycelium all can be early than sporogenesis, growth under general environment, can win initial success through 7 to 10 days, along with the sprouting breeding of spore, effect is just more obvious later on; The 4th, test shows, this microbial inoculum control bacterium spectrum is wider, control efficiency is better, can be widely used in cereal crops and economic crops such as barley, corn, cotton, peanut, strawberry, tomato, cucumber, broccoli, cauliflower, onion, effectively prevent and treat stalk break, diseases such as fusarium wilt, damping off (seeing Table 1)
Table 1 pair stalk break, the control efficiency of diseases such as fusarium wilt, damping off
| Prevent and treat disease | Pathogen | Control efficiency (%) |
| The Solanaceae stalk break | ????Sclerotinia?sclerotiorum | ????70～95 |
| Bighead atractylodes rhizome southern blight | ????Sclerotium?rolfsii | ????70～95 |
| Cucumber fusarium axysporum | ????Fusarium?oxysporum | ????60～80 |
| Cotton seedling blight | ????Rhizoctonia?solan | ????70～90 |
| Melon damping off | ????Pythium?aphanidermatum | ????70～90 |
Description of drawings
Accompanying drawing is a production procedure schematic diagram of the present invention
Embodiment
The invention will be further described below in conjunction with accompanying drawing.
Fungus Trichoderma biological control granular bacteria preparation of the present invention is made up of the single bacterial classification of fungus Trichoderma or composite bacteria pure culture viable bacteria and granular solids medium thing and seed coating medicine, concrete preparation process, and technology is carried out according to accompanying drawing production procedure schematic diagram.
Embodiment 1:
(1) separation of trichoderma Trichoderma viride (Trichoderma vivid) bacterial classification, purifying and cultivation: by Fang Zhongda (the agriculture publishing house of " planting the disease research method ", published in 1979) described conventional method, from field crops or soil, carrying out orientation separates, subsequent purificn and cultivation reach 100% purity and do not have other assorted bacterium to bacteria containing amount.
(2) preparation of granular solids medium thing: as raw material, from its broken offcuts, filter out particle diameter 1-5mm particle with rice, drop in the high-pressure steam sterilizing pan,, about 120 ℃, sterilize after 30 minutes, cool off under the room temperature by 15 pounds of pressure;
(3) bacterial classification inoculation of granular solids medium thing and fermented and cultured: equipment, behind the sterilization of place, with the trichoderma viride of above-mentioned separation, purifying, cultivation and the cooled basic thing of sterilizing, mix and inoculation by 1: 200 part by weight at normal temperatures, at room temperature 20-30 ℃, under the humidity 65-90% condition, fermented and cultured 4-7 days, when treating that trichoderma viride breeding is covered with the rice pellets surface;
(4) preparation of seed coating medicine:
(a) with sodium carboxymethylcellulose (CMC) (chemical reagent station, Shanghai packing factory product) and sterile water in 1 (gram weight): 1000 (milliliter volume) ratio mixes into the scattered paste shape binding agent;
(b) be raw material with the wheat, after attrition process, cross the micropowders of 250～360 mesh sieves as coating powder;
(c) with above-mentioned coating powder and binding agent in 50 (gram weight): 3 (milliliter volume) ratio is mixed mutually, makes the scattered paste shape seed coating medicine through stirring.
(5) the carry disease germs dressing of granular solids medium thing, drying and packing: will be through inoculation, the granular rice of fermented and cultured is poured in an amount of scattered paste shape seed coating medicine, after stirring, pull out and spread out, air-dry with normal temperature air-flow air-dry machine, after the drying, weighing, and with the chemical fibre woven bag pack product, store at normal temperatures and turn round.
Embodiment 2:
(1) the frequent under field conditions (factors) and Trichoderma harzianum (Trichoderma harzianum) of trichoderma trichoderma viride (Trichoderma vivid), yellow green trichoderma (Trichoderma aureoviride), among the tangerine green trichoderma (Trichoderma citrinouiride) one or two or more kinds mixed and given birth to together, compile the described conventional method of " planting a disease research method " book by Fang Zhongda, above-mentioned any mixed living bacterial classification from field crops or soil, carrying out orientation separates, subsequent purificn and cultivation reach 100% purity and do not have other assorted bacterium to bacteria containing amount.
(2) be major ingredient with the corn flour, add an amount of sodium carboxymethyl starch and water through mixing granulation, after the drying, therefrom filter out the particle of particle diameter 1-5mm, after sterilization, the cooling back becomes the solid culture medium thing under the room temperature;
(3 with above-mentioned composite bacteria and corn particle shape solid culture medium thing, and mix by 1: 300 part by weight at normal temperatures and inoculate, 20～30 ℃ of room temperatures, under humidity 65～90% conditions, after fermented and cultured 4-7 days;
(4) preparation of seed coating medicine:
(a) with sodium carboxymethyl starch and aqua sterilisa in 3 (gram weight): 1000 (milliliter volume) ratio mixes into binding agent;
(b) be that to be processed into the ultra micro powder be coating powder to raw material with rice;
(c) with above-mentioned coating powder and binding agent in 90 (gram weight): 10 (milliliter volume) ratio is mixed mutually, is stirred and make seed coating medicine;
(5) the corn particle shape solid culture medium thing that will carry disease germs is poured into and is carried out dressing in an amount of seed coating medicine, drying, packing product, method is with embodiment 1.
Claims (6)
1. fungus Trichoderma biological and ecological methods to prevent plant disease, pests, and erosion granular bacteria preparation is characterized in that being made up of fungus Trichoderma pure culture viable bacteria and granular solids medium thing and seed coating medicine.
2. according to the described fungus Trichoderma biological and ecological methods to prevent plant disease, pests, and erosion of claim 1 granular bacteria preparation, it is characterized in that described fungus Trichoderma bacterial classification is Trichoderma viride (Trichoderma vivid) or this bacterial classification and Trichoderma harzianum (Trichodermaharzianum), yellow green trichoderma (Trichoderma auxeoviride), a kind of among the tangerine green trichoderma (Trichodermacitrinouirier) or some kinds are pressed the compound fungi strain of arbitrary proportion.
3. according to the described fungus Trichoderma biological and ecological methods to prevent plant disease, pests, and erosion of claim 1 granular bacteria preparation, it is characterized in that described granular solids medium thing is the particle diameter particle of choosing any one kind of them or is the formulated the same granular formulations of particle diameter of raw material and other auxiliary material with this base thing powder among the millet between the 1-5mm, rice, corn, barley and wheat or jowar.
4. according to the described fungus Trichoderma biological and ecological methods to prevent plant disease, pests, and erosion of claim 1 granular bacteria preparation, it is characterized in that described seed coating medicine is mixed by coating powder and binding agent.
5. according to claim 1 or 4 described fungus Trichoderma biological and ecological methods to prevent plant disease, pests, and erosion granular bacteria preparation, it is characterized in that described coating powder is the ultra micro powder of choosing any one kind of them and being processed into for raw material among the above-mentioned medium thing, wherein wheat or rice ultra micro powder are preferred feedstock; Described binding agent is the mixture of sodium carboxymethylcellulose (CMC) or sodium carboxymethyl starch and water.
6. according to the preparation method of the described fungus Trichoderma biological and ecological methods to prevent plant disease, pests, and erosion of claim 1 granular bacteria preparation, it is characterized in that being prepared from through following machining process:
(1) separation of fungus Trichoderma bacterial classification, purifying and cultivation reach 100% purity and do not have other assorted bacterium to bacteria containing amount;
(2) preparation of granular solids medium thing: will select the particle of basic thing or the granular formulations that is mixed with this base thing powder, therefrom filter out the particle of particle diameter at 1-5mm, at 15-20 pound pressure, 120 ℃ of left and right sides high pressure steam sterilizations cooled off under the room temperature after 30 minutes;
(3) bacterial classification inoculation of granular solids medium thing and fermented and cultured: after the equipment disinfection sterilization, under aseptic condition, bacterial classification and granular solids medium thing are inoculated by 1: 200～300 part by weight at normal temperatures, 20～30 ℃ of room temperatures, under humidity 65～90% conditions, fermented and cultured 4～7 days;
(4) preparation of seed coating medicine: (a) with above-mentioned sodium carboxymethylcellulose (CMC) or sodium carboxymethyl starch and water in 1～3 (gram weight): 1000 (milliliter volume) ratio is mixed mutually, stir into scattered paste shape binding agent; (b) above-mentioned optional basic thing is crossed 250～360 mesh sieves after attrition process and be ultra micro shape coating powder; (c) with coating powder and scattered paste shape binding agent in 50～100 (gram weight): 1～10 (milliliter volume) ratio is mixed mutually, makes seed coating medicine through stirring.
(5) the carry disease germs dressing of granular solids culture, dry and packing: the granular solids medium thing that will connect behind the strain fermentation is poured in an amount of seed coating medicine, pull out after stirring and spread out, with the air-dry back of normal temperature air-flow pack product.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100482081C (en) * | 2006-07-21 | 2009-04-29 | 中国科学院生态环境研究中心 | Biocontrol trichoderma agent prepared with active sludge and abandoned biomass |
| CN101779678A (en) * | 2009-01-20 | 2010-07-21 | 北京嘉博文生物科技有限公司 | Biological sweet sorghum seed pelleting agent and preparation method thereof |
| CN102696688A (en) * | 2012-05-30 | 2012-10-03 | 上海交通大学 | Preparation method of trichoderma sorghum granules |
| CN103039439A (en) * | 2011-10-11 | 2013-04-17 | 上海万力华生物科技有限公司 | Seed coating agent of Trichoderma fungi and preparation method thereof |
| CN103131642A (en) * | 2013-03-18 | 2013-06-05 | 济南巨微生物技术有限公司 | Trichoderma viride and culture method and application thereof |
| CN103320330A (en) * | 2012-12-25 | 2013-09-25 | 山东省农业科学院农产品研究所 | Trichoderma aureoviride strain and application thereof |
| CN104211630A (en) * | 2013-05-30 | 2014-12-17 | 福州大学 | Alkaloid compound from Trichoderma citrinoviride and application thereof |
| CN104788175A (en) * | 2015-03-30 | 2015-07-22 | 上海交通大学 | Biological trichoderma viride granule as well as preparation method and application thereof |
| CN105779304A (en) * | 2016-03-25 | 2016-07-20 | 山东省科学院生态研究所 | Culture medium for screening trichoderma strains for preventing and controlling botrytis and sclerotinia sclerotiorum |
| CN106424127A (en) * | 2016-09-05 | 2017-02-22 | 青岛农业大学 | Repair method of phthalic acid ester contaminated soil |
| CN109042742A (en) * | 2018-08-18 | 2018-12-21 | 杭州渔森农业技术开发有限公司 | Compound formulation is used to prevent and treat soil and passes the purposes fallen ill |
| CN109414024A (en) * | 2016-04-28 | 2019-03-01 | 组合化学工业株式会社 | Pesticidal preparations composition, preparation method and the application method of trichoderma bacterium are used |
| CN109438087A (en) * | 2018-12-27 | 2019-03-08 | 天津环微生物科技有限公司 | A kind of instant granular type agricultural microbial agent and preparation method and application method |
| CN110810440A (en) * | 2019-11-13 | 2020-02-21 | 河南农贝得农业科技有限公司 | Microbial agent for preventing and treating crop southern blight and preparation method thereof |
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2003
- 2003-12-19 CN CN 200310124121 patent/CN1257672C/en not_active Expired - Fee Related
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100482081C (en) * | 2006-07-21 | 2009-04-29 | 中国科学院生态环境研究中心 | Biocontrol trichoderma agent prepared with active sludge and abandoned biomass |
| CN101779678A (en) * | 2009-01-20 | 2010-07-21 | 北京嘉博文生物科技有限公司 | Biological sweet sorghum seed pelleting agent and preparation method thereof |
| CN101779678B (en) * | 2009-01-20 | 2013-03-27 | 北京嘉博文生物科技有限公司 | Biological sweet sorghum seed pelleting agent and preparation method thereof |
| CN103039439A (en) * | 2011-10-11 | 2013-04-17 | 上海万力华生物科技有限公司 | Seed coating agent of Trichoderma fungi and preparation method thereof |
| CN103039439B (en) * | 2011-10-11 | 2015-04-15 | 上海万力华生物科技有限公司 | Seed coating agent of Trichoderma fungi and preparation method thereof |
| CN102696688A (en) * | 2012-05-30 | 2012-10-03 | 上海交通大学 | Preparation method of trichoderma sorghum granules |
| CN103320330B (en) * | 2012-12-25 | 2015-02-04 | 山东省农业科学院农产品研究所 | Trichoderma aureoviride strain and application thereof |
| CN103320330A (en) * | 2012-12-25 | 2013-09-25 | 山东省农业科学院农产品研究所 | Trichoderma aureoviride strain and application thereof |
| CN103131642A (en) * | 2013-03-18 | 2013-06-05 | 济南巨微生物技术有限公司 | Trichoderma viride and culture method and application thereof |
| CN103131642B (en) * | 2013-03-18 | 2014-07-23 | 赵钦刚 | Trichoderma viride and culture method and application thereof |
| CN104211630A (en) * | 2013-05-30 | 2014-12-17 | 福州大学 | Alkaloid compound from Trichoderma citrinoviride and application thereof |
| CN104211630B (en) * | 2013-05-30 | 2016-05-04 | 福州大学 | A kind of alkaloid compound and application thereof that comes from tangerine green trichoderma |
| CN104788175A (en) * | 2015-03-30 | 2015-07-22 | 上海交通大学 | Biological trichoderma viride granule as well as preparation method and application thereof |
| CN105779304A (en) * | 2016-03-25 | 2016-07-20 | 山东省科学院生态研究所 | Culture medium for screening trichoderma strains for preventing and controlling botrytis and sclerotinia sclerotiorum |
| CN109414024A (en) * | 2016-04-28 | 2019-03-01 | 组合化学工业株式会社 | Pesticidal preparations composition, preparation method and the application method of trichoderma bacterium are used |
| CN109414024B (en) * | 2016-04-28 | 2021-10-19 | 组合化学工业株式会社 | Pesticide preparation composition using trichoderma, preparation method and application method thereof |
| CN106424127A (en) * | 2016-09-05 | 2017-02-22 | 青岛农业大学 | Repair method of phthalic acid ester contaminated soil |
| CN109042742A (en) * | 2018-08-18 | 2018-12-21 | 杭州渔森农业技术开发有限公司 | Compound formulation is used to prevent and treat soil and passes the purposes fallen ill |
| CN109438087A (en) * | 2018-12-27 | 2019-03-08 | 天津环微生物科技有限公司 | A kind of instant granular type agricultural microbial agent and preparation method and application method |
| CN110810440A (en) * | 2019-11-13 | 2020-02-21 | 河南农贝得农业科技有限公司 | Microbial agent for preventing and treating crop southern blight and preparation method thereof |
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