CN109168874A - A kind of method that Abies ziyuanensis in two populations artificial pollination promotes Wild population seedlings regeneration - Google Patents
A kind of method that Abies ziyuanensis in two populations artificial pollination promotes Wild population seedlings regeneration Download PDFInfo
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- CN109168874A CN109168874A CN201811070273.8A CN201811070273A CN109168874A CN 109168874 A CN109168874 A CN 109168874A CN 201811070273 A CN201811070273 A CN 201811070273A CN 109168874 A CN109168874 A CN 109168874A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention discloses a kind of methods that Abies ziyuanensis in two populations artificial pollination promotes Wild population seedlings regeneration comprising the steps of: pollen collecting: when the male cone of 80%-90% on male cone brachyplast turns yellow, starting to collect pollen；Pollen storage: pollen is packed into Brown Glass Brown glass bottles and jars only, is stored under the conditions of 4 DEG C；It builds pollination operation rack: building pollination operation rack around the tree crown of Abies ziyuanensis in two populations pollination elite stand；Pollination: the 6th~10 day after collecting pollen, female cone bract scale is pollinated when opening a business；Bagging: to put on pollination bag immediately after female cone pollination, and pollination bag is tightened；Remove bag: pollination extractd pollination bag after 10~15 days.The present invention is for the flowering asynchronism of endangered plant Abies ziyuanensis in two populations male and female, spontaneous pollination is bad, seed height abortion leads to field seedlings regeneration disruption, a kind of artificial pollination method being simple and efficient is provided, Abies ziyuanensis in two populations seed plumpness greatly improved, Abies ziyuanensis in two populations Wild population is promoted to restore seedlings regeneration ability.
Description
Technical field
The invention belongs to endangered plants guard method technical field, specially a kind of Abies ziyuanensis in two populations artificial pollination promotes field
The method of population seedlings regeneration.
Background technique
Seedlings regeneration is the process that plant sprouts and settles down from seed production to seedling, and seedlings regeneration is plant population supplement
Newborn individual enables population to increase or maintain to stablize.In addition, because seedlings regeneration is sexual reproduction process, the filial generation of production
A physical efficiency generates certain hereditary variation, and species can be promoted constantly to evolve to adapt to environmental change.Therefore, seedlings regeneration is dimension
It is the highly important basis of species viability.If obstacle occurs in seedlings regeneration, population does not have the supplement of newborn individual, a side
Face, as individual is died off in population, the individual amount of population is gradually reduced, on the other hand, due to not having hereditary variation
The evolution potential of the replacement of body, population is stagnated, and is adapted to the ability decline of environmental change, is led to population continuous downturn.One kind is planted
For object, if seedlings regeneration obstacle long-term existence, the plant will sink into critically endangered state, until final extinction.It influences wild
The factor of outer seedlings regeneration mainly includes provenance and environmental condition, and enough provenances are the indispensable items that plant realizes seedlings regeneration
Part.
Abies ziyuanensis in two populations (Abiesziyuanensis) be the distinctive endangered plants of China, by " Chinese Plants Red Data Book " and
" national key protected wild plants register (first) " is classified as focused protection species.The population of plant remaining is very rare,
Only have on Guangxi Ziyuan County Yinzhulaoshan, Hunan Xinning County Shun Mount Huang, Hunan Yanling County compound forest farm and Jinggangshan County, Jiangxi Province and other places
A small amount of distribution, plant lazy weight 600 are in highly endangered situation.Abies ziyuanensis in two populations is since 1978 are found, field kind
Not yet there is seedling in group, there are serious obstacles for seedlings regeneration.Currently, Abies ziyuanensis in two populations field seedlings regeneration is promoted mainly to lead to
It crosses and removes forest lower layer excessively intensive shrub, draft and litter, the seed fallen from elite stand is allow to contact soil,
It is promoted to sprout and settle down, but this method fails that Abies ziyuanensis in two populations Wild population is promoted seedling sprouting occur always.
The present inventor studies for a long period of time discovery, Abies ziyuanensis in two populations the phenomenon that there are male and female flowering asynchronisms, and Post flowering is caused to be awarded naturally
Powder is seriously bad, naturally solid seed height abortion, therefore, only solid by spontaneous pollination, it will be difficult to realize Abies ziyuanensis in two populations open country
The seedlings regeneration of outer population.And artificial pollination can overcome male and female flowering asynchronism and reduce the influence etc. of florescence adverse weather,
Seed plumpness can be greatly improved, provides effective provenance for Abies ziyuanensis in two populations Wild population seedlings regeneration.Up to the present, not yet
The method that document or patent report Abies ziyuanensis in two populations artificial pollination promote field seedling natural renovation.
Summary of the invention
The present invention is excessively high for the naturally solid seeds abortion rate of Abies ziyuanensis in two populations, causes the seedlings regeneration of Wild population long-term
The problem of interruption, a kind of method for disclosing artificial pollination, it is intended to obtain a kind of convenience and low cost, it is full that its seed can be effectively improved
Full scale promotes the method for Abies ziyuanensis in two populations Wild population seedlings regeneration.
To achieve the above object, technical solution provided by the invention is as follows:
A kind of method that Abies ziyuanensis in two populations artificial pollination promotes Wild population seedlings regeneration, including following operation:
(1) when the male cone of 80-90% on male inflorescence brachyplast switchs to yellow by yellow green, it is short pollen collecting: to cut male inflorescence
Branch, insertion are equipped with the bottle of clear water, the male cone of small part on spray or green and yellow green are all extractd, then water
Bottle moves on on a clean A4 specification blank sheet of paper, and the yellow pollen of Spontaneous release is allowed to trickle down on blank sheet of paper, collects within every 6 hours primary
Pollen on blank sheet of paper, it is continuous to collect 2 days；
(2) pollen stores: pollen being packed into Brown Glass Brown glass bottles and jars only, covers tightly bottle cap, is stored under the conditions of 4 DEG C；
(3) it builds pollination operation rack: building pollination operation rack around the tree crown of Abies ziyuanensis in two populations pollination elite stand；
(4) it pollinates: the 6th~10 day after collecting pollen, pollinating when the bract scale that female cone is closed originally is opened a business；
(5) bagging: pollination bag is put on immediately after pollinating to female cone, and tightens pollination bag；
(6) remove bag: pollination extractd pollination bag after 10~15 days.
In step (1), when collecting pollen on blank sheet of paper, to avoid pollen by scraped finish or rolling equivalent damage, flower arrangement is first removed
The water bottle of branch, then two, paper diagonal slightly doublings are caught, back side of paper is then softly patted, pollen is made to be slipped to the folding of blank sheet of paper
In seam, pollen is softly poured into the Brown Glass Brown glass bottles and jars only of dried and clean later.
Abies ziyuanensis in two populations is diclinism plant, by largely observe and it is demonstrated experimentally that male cone only it is raw in tree crown
On the branch of portion and lower part, since the illumination that the male cone in the middle part of tree crown obtains is more sufficient, pollen quality is than tree crown lower part
Obtain the weaker male cone of illumination wants high.To improve Abies ziyuanensis in two populations pollination rate, best Pollination Effect is obtained, is determined preferred
Male inflorescence brachyplast is the good branch of illumination in the middle part of tree crown.
Abies ziyuanensis in two populations male cone is cylindricality spike, and a male cone includes about 60~200 anther, and anther development is early
Phase is green, and development mid-term switchs to yellow green, and development later stage switchs to yellow.By largely observing and it is demonstrated experimentally that male cone
Color when changing into yellow by yellow green, pollen has been reached maturity, and anther will crack loose powder at this time.If changed into male cone
It cuts flowers before yellow, then the pollen collected not yet is reached maturity, if male cone is waited all to change into ability clip after yellow, portion
Potted flower medicine has shed pollen, and the yield of pollen is greatly reduced, and learns from many experiments summary, clip male cone brachyplast it is best
Time is when on brachyplast 90% or so male cone changes into yellow by yellow green.
In step (2), the pollen stored under the conditions of being 4 DEG C of guarantee is able to maintain higher vigor, using following two method
One or both of:
1) polliniferous Brown Glass Brown glass bottles and jars only will be filled and is put into the refrigerator with dehumidification function, stored under the conditions of 4 DEG C；
2) polliniferous Brown Glass Brown glass bottles and jars only will be filled and is put into plastic seal pocket, discoloration silica-gel desiccant 50~150 is added in sealed bag
Gram, it is tamping after sack under the conditions of being put into 4 DEG C and stores, when the water suction of the discoloration silica gel particle of blue becomes light blue or pink,
The discoloration silica gel more renewed in time.
In step (3), to pollinate to female cone as much as possible, when building pollination operation rack, height is to grasp
Author station stretches out one's hand most of female cones on accessible tree crown on rack to be advisable.
The female cone of Abies ziyuanensis in two populations is short cylindrical shape inflorescence, is learnt by largely observing, and female cone is erected raw in tree crown
On nearly top to the branch at top, since the height of Abies ziyuanensis in two populations bearing tree is generally at 15 meters or more, need to build rack ability
Implement pollination.
The size of Abies ziyuanensis in two populations elite stand has a major impact the seed plumpness of Abies ziyuanensis in two populations artificial pollination, and the diameter of a cross-section of a tree trunk 1.3 meters above the ground is greater than 30
Centimetre elite stand, higher seed plumpness, elite stand of the diameter of a cross-section of a tree trunk 1.3 meters above the ground at 15 centimetres to 30 centimetres, seed after pollination can be obtained after pollination
Plumpness is decreased obviously, and elite stand of the diameter of a cross-section of a tree trunk 1.3 meters above the ground less than 15 centimetres, and since tree age is small and growth potential is weak etc., factors influence, and is awarded
Seed plumpness is very low after powder, therefore preferably elite stand of the diameter of a cross-section of a tree trunk 1.3 meters above the ground greater than 30 centimetres pollinates, and builds pollination around its tree crown
Operate rack.
In step (4), the Brown Glass Brown glass bottles and jars only of storage pollen in step (2) is put into foam preservative box by the pollination same day, fresh-keeping
The biological ice bag that 2 frosts are put into box keeps low temperature, is carried under pollination elite stand after closing tight box cover, operates rack by pollination
It rises to by the female cone branch on tree crown top.
Specific pollination operation are as follows: vial is held with left hand middle finger, the third finger and little finger of toe, thumb and index finger clamp female cone
Raw small stems keep stablizing, the right hand holds clean writing brush and protrudes into vial, and writing brush head is made to be stained with pollen, then light with writing brush head
Soft click female cone surface, clicks and enters pollen grain in the bract scale mouth opened a business on female cone, and bract scale mouth to be opened a business is entered pollen
Afterwards, pollination terminates.
After pollination, upper label is covered in pollination female cone base portion, writes male parent and maternal and pollination time exactly on label.
By largely observe and it is demonstrated experimentally that the Abies ziyuanensis in two populations florescence have protandry feature, i.e., in pollen maturation
And when discharging, the ovule of female flower is not yet reached maturity, and female flower, which does not enter into, to be awarded the phase, should not be pollinated at this time.Abies ziyuanensis in two populations
Female cone be cylindrical inflorescence, above helical form raw 80~200 bract scales, 1 cone-scale is associated on the inside of 1 bract scale, in pearl
Squama base portion have 2 pieces of ovules, the 6th~10 day after pollen maturation and release, Ovule Development is mature, significant to be characterized in
Originally the bract scale that closes is opened a business an osculum out, and female flower can carry out artificial pollination into that can award the phase at this time.Accordingly, it is preferred that awarding
The powder time is the 6th~10 day collected after pollen, when the bract scale closed originally is opened a business.
Since just as the spring rain time, female cone is not soaked with rain when to guarantee pollination during pollination, with improve pollination at
Power entangles female cone in pollination proxima luce (prox. luc) with template pouch, avoids female cone from being soaked with rain, when next day pollinates, first use
Rain gear shelters from rainwater above female cone, plucks the template pouch covered on female cone later, then pollinates, and completes institute
After having female cone pollination, rain gear is just removed.
Using the benefit of template bagging: template papery is pure, intensity is high, transparent good, waterproof, indeformable, can have
Effect protects female cone not to be soaked with rain.
In step (6), after pollination 6 months, Abies ziyuanensis in two populations cone turns yellowish-brown by green, in the cone Jing Guo artificial pollination
Middle random acquisition 10 peels seed respectively and carries out dissection and check whether there is or not embryo, counts the seed plumpness of artificial pollination cone.
Remaining cone Jing Guo artificial pollination stays in the tree, gives free rein to fall off and sow.To April next year, check around artificial pollinizers
Earth's surface fir growth of seedling situation counts Abies ziyuanensis in two populations seedling numbers.
The beneficial effects of the present invention are:
(1) present invention is bad for Abies ziyuanensis in two populations spontaneous pollination, seeds abortion rate is excessively high, causes the seedlings regeneration of Wild population long
Interim disconnected problem provides a kind of method of Abies ziyuanensis in two populations artificial pollination, easy to operate, efficient, pollination rate and seed plumpness
Height can effectively facilitate Abies ziyuanensis in two populations Wild population and restore seedlings regeneration, mention for protection and sustainable utilization Abies ziyuanensis in two populations germ plasm resource
For technical support.
(2) present invention has determined a kind of suitable Abies ziyuanensis in two populations people according to the feature of Abies ziyuanensis in two populations florescence protandry
The method of work pollination, it is simple and fast, it is easy to operate, elite stand successful fertilization can be made, the seed plumpness for cone of pollinating is very high, can
Obtain the seed of a large amount of tool sprouting vigor.This method obtain vibrant seed, in addition to be able to satisfy Abies ziyuanensis in two populations seedlings regeneration and
Research work needs, and can also be Abies ziyuanensis in two populations breeding and afforestation, realize that the development and utilization of the plant resources provide critical kind
Source.
(3) it is found by experimental result, the healthy and strong elite stand that Abies ziyuanensis in two populations pollination will select the diameter of a cross-section of a tree trunk 1.3 meters above the ground to be greater than 30 centimetres, after pollination
Higher seed plumpness can be obtained, and diameter of a cross-section of a tree trunk 1.3 meters above the ground seed plumpness after 15 centimetres to 30 centimetres of elite stand, pollination is decreased obviously,
When the elite stand diameter of a cross-section of a tree trunk 1.3 meters above the ground is less than 15 centimetres, seed plumpness is very low after pollination, therefore present invention determine that the excellent strain mark of pollination elite stand
It will definitely be applied to Abies ziyuanensis in two populations artificial pollination breeding.
Detailed description of the invention
Fig. 1 is Abies ziyuanensis in two populations male cone form.
Fig. 2 is Abies ziyuanensis in two populations pollen collecting state.
Fig. 3 is Abies ziyuanensis in two populations female cone bract scale the first transaction of a day's business form.
Fig. 4 is the Abies ziyuanensis in two populations female cone pollination bag-shaped condition of rear enclosure.
Specific embodiment
The content of present invention is further described below with reference to embodiment and attached drawing, but is not limitation of the invention.
Embodiment 1
A kind of method that Abies ziyuanensis in two populations artificial pollination promotes Wild population seedling natural renovation, steps are as follows:
(1) pollen collecting: using the Abies ziyuanensis in two populations of Guangxi resource Yinzhulaoshan Nature Reserve as pollen source and pollination pair
As.The male cone and female cone of Abies ziyuanensis in two populations at the beginning of 4 months are quickly expanded, as shown in Figure 1, on male cone brachyplast about 90% male ball
Flower is changed into the 11:30-12:30 on the yellow same day by yellow green, clip the brachyplast of growth male cone sequence, take back conservation of nature rapidly
Management station of area, insertion are equipped with the bottle of clear water, the male cone of small part on brachyplast or yellow green are all extractd, then water
Bottle moves on on a clean A4 specification blank sheet of paper.Pollen started to discharge on 2nd, and the yellow pollen of Spontaneous release is trickled down in blank sheet of paper
On, collection in every 6 hours once trickles down the pollen on blank sheet of paper, continuous collection 2 days, as shown in Figure 2.
(2) pollen stores: when collecting pollen, to avoid pollen by scraped finish or rolling equivalent damage, first removing flower arrangement branch
Water bottle, then two, paper diagonal slightly doublings are caught, back side of paper is then softly patted, pollen is made to be slipped to the crease of blank sheet of paper
In, pollen is softly poured into the Brown Glass Brown glass bottles and jars only of dried and clean later, covers tightly 4 DEG C of refrigerator be put into after bottle cap with dehumidifier function
Storage.Terminate substantially to the release of 18:00 afternoon on the 3rd or so pollen.
(3) it builds pollination operation rack: the 3rd day after clip male cone brachyplast, selecting female cone quantity more, and chest
Diameter is that 32 centimetres of Abies ziyuanensis in two populations is pollination elite stand, builds pollination rack around its tree crown, climbs up pollination rack daily later and sees
Examine female cone developmental stage.
(4) the 5th day after clip male cone, bract scale goes out the sign for being now ready for opening a business on female cone, as shown in figure 3, with sulfuric acid
Paper pouch entangles female cone, as shown in figure 4, female cone is avoided to be soaked with rain.
(5) the 6th day 9:00 in morning observes female cone after clip male cone, confirms that bract scale has been opened a business on female cone.Then
The Brown Glass Brown glass bottles and jars only of preservation pollen is taken out refrigerator, foam preservative box is moved rapidly into and the biological ice bag for putting 2 frosts keeps low
Temperature is carried under the Abies ziyuanensis in two populations elite stand of quasi- pollination after closing tight crisper cover board, after riseing onto pollination rack, is first hidden with rain gear
It blocks above female cone, avoids rainwater from trickling down on female cone, pluck the template pouch covered on female cone later, from fresh-keeping
Box taking-up fills polliniferous Brown Glass Brown glass bottles and jars only, holds vial with left hand middle finger, the third finger and little finger of toe, thumb and index finger are clamped female
Globe daisy raw small stems keep stablizing, the right hand holds clean writing brush and protrudes into vial, so that writing brush head is stained with pollen, then with writing brush
Head is soft to click female cone surface, pollen grain is clicked and entered the bract scale mouth opened a business on female cone, bract scale mouth to be opened a business is entered
After pollen, female cone is entangled from top to bottom with pollination bag, is blown away to avoid fitful wind or pollen on female cone is fallen in rain drop erosion, pricked
Tight pollination bag, hangs up tag board, and pollination male parent, female parent and pollination date are write on tag board, extracts and is covered in female ball after 10 days
The pollination bag taken.
(6) late October current year Abies ziyuanensis in two populations cone turns yellowish-brown by green, October 21, by artificial pollination
Random acquisition 10 in cone peel seed respectively and carry out dissection and check that the seed for counting artificial pollination cone whether there is or not embryo is full
Full scale.Remaining cone Jing Guo artificial pollination stays in the tree, gives free rein to fall off and sow.To April next year, artificial pollinizers are checked
Peripheral ground fir growth of seedling situation counts Abies ziyuanensis in two populations seedling numbers.
Embodiment 2
A kind of method that Abies ziyuanensis in two populations artificial pollination promotes Wild population seedling natural renovation, operating procedure are as follows:
(1) pollen collecting: using the Abies ziyuanensis in two populations of Guangxi resource Yinzhulaoshan Nature Reserve as pollen source and pollination pair
As.The male cone and female cone of Abies ziyuanensis in two populations at the beginning of 4 months are quickly expanded, and about 90% male cone is by yellow green on male cone brachyplast
Change into the 11:30-12:30 on the yellow same day, clip the brachyplast of raw male cone, take back nature reserve management station rapidly, be inserted into
Bottle equipped with clear water, by the male cone of small part on brachyplast or yellow green all extract, then water bottle move on to one it is clean
On net A4 specification blank sheet of paper.Pollen started to discharge on 2nd, and the yellow pollen of Spontaneous release is trickled down on blank sheet of paper, collected within every 6 hours
The pollen on blank sheet of paper once is trickled down, it is continuous to collect 2 days.
(2) pollen stores: when collecting pollen, to avoid pollen by scraped finish or rolling equivalent damage, first removing flower arrangement branch
Water bottle, then two, paper diagonal slightly doublings are caught, back side of paper is then softly patted, pollen is made to be slipped to the crease of blank sheet of paper
In, pollen is softly poured into the Brown Glass Brown glass bottles and jars only of dried and clean later, covers tightly 4 DEG C of refrigerator be put into after bottle cap with dehumidifier function
Storage.Terminate substantially to the release of 18:00 afternoon on the 3rd or so pollen.
(3) it builds pollination operation rack: the 4th day after clip male cone brachyplast, selecting female cone quantity more, and chest
Diameter is that 35 centimetres of Abies ziyuanensis in two populations is pollination elite stand, builds pollination rack around its tree crown, climbs up pollination rack daily later and sees
Examine female cone developmental stage.
(4) the 7th day after clip male cone, bract scale goes out the sign for being now ready for opening a business on female cone, is entangled with template pouch
Female cone avoids female cone from being soaked with rain.
(5) the 8th day 9:00 in morning observes female cone after clip male cone, confirms that bract scale has been opened a business on female cone.Then
The Brown Glass Brown glass bottles and jars only of preservation pollen is taken out refrigerator, foam preservative box is moved rapidly into and the biological ice bag for putting 2 frosts keeps low
Temperature is carried under the Abies ziyuanensis in two populations elite stand of quasi- pollination after closing tight crisper cover board, after riseing onto pollination rack, is first hidden with rain gear
It blocks above female cone, avoids rainwater from trickling down on female cone, pluck the template pouch covered on female cone later, from fresh-keeping
Box taking-up fills polliniferous Brown Glass Brown glass bottles and jars only, holds vial with left hand middle finger, the third finger and little finger of toe, thumb and index finger are clamped female
Globe daisy raw small stems keep stablizing, the right hand holds clean writing brush and protrudes into vial, so that writing brush head is stained with pollen, then with writing brush
Head is soft to click female cone surface, pollen grain is clicked and entered the bract scale mouth opened a business on female cone, bract scale mouth to be opened a business is entered
After pollen, female cone is entangled from top to bottom with pollination bag, is blown away to avoid fitful wind or pollen on female cone is fallen in rain drop erosion, pricked
Tight pollination bag, hangs up tag board, and pollination male parent, female parent and pollination date are write on tag board, extracts and is covered in female ball after 13 days
The pollination bag taken.
(6) late October current year Abies ziyuanensis in two populations cone turns yellowish-brown by green, October 22, by artificial pollination
Random acquisition 10 in cone peel seed respectively and carry out dissection and check that the seed for counting artificial pollination cone whether there is or not embryo is full
Full scale.Remaining cone Jing Guo artificial pollination stays in the tree, gives free rein to fall off and sow.To April next year, artificial pollinizers are checked
Peripheral ground fir growth of seedling situation counts Abies ziyuanensis in two populations seedling numbers.
Embodiment 3
A kind of method that Abies ziyuanensis in two populations artificial pollination promotes Wild population seedling natural renovation, operating procedure are as follows:
(1) pollen collecting: using the Abies ziyuanensis in two populations of Guangxi resource Yinzhulaoshan Nature Reserve as pollen source and pollination pair
As.The male cone and female cone of Abies ziyuanensis in two populations at the beginning of 4 months are quickly expanded, and about 90% male cone is by yellow green on male cone brachyplast
Change into the 11:30-12:30 on the yellow same day, clip the brachyplast of raw male cone, take back nature reserve management station rapidly, be inserted into
Bottle equipped with clear water, by the male cone of small part on brachyplast or yellow green all extract, then water bottle move on to one it is clean
On net A4 specification blank sheet of paper.Pollen started to discharge on 2nd, and the yellow pollen of Spontaneous release is trickled down on blank sheet of paper, collected within every 6 hours
Once trickle down the pollen on blank sheet of paper.
(2) pollen stores: when collecting pollen, to avoid pollen by scraped finish or rolling equivalent damage, first removing flower arrangement branch
Water bottle, then two, paper diagonal slightly doublings are caught, back side of paper is then softly patted, pollen is made to be slipped to the crease of blank sheet of paper
In, pollen is softly poured into the Brown Glass Brown glass bottles and jars only of dried and clean later, covers tightly 4 DEG C of refrigerator be put into after bottle cap with dehumidifier function
Storage.Terminate substantially to the release of 18:00 afternoon on the 3rd or so pollen.
(3) it builds pollination operation rack: the 5th day after clip male cone brachyplast, selecting female cone quantity more, and chest
Diameter is that 31 centimetres of Abies ziyuanensis in two populations is pollination elite stand, builds pollination rack around its tree crown, climbs up pollination rack daily later and sees
Examine female cone developmental stage.
(4) the 8th day after clip male cone, bract scale goes out the sign for being now ready for opening a business on female cone, is entangled with template pouch
Female cone avoids female cone from being soaked with rain.
(5) the 9th day 9:00 in morning observes female cone after clip male cone, confirms that bract scale has been opened a business on female cone.Then
The Brown Glass Brown glass bottles and jars only of preservation pollen is taken out refrigerator, foam preservative box is moved rapidly into and the biological ice bag for putting 2 frosts keeps low
Temperature is carried under the Abies ziyuanensis in two populations elite stand of quasi- pollination, after riseing onto pollination rack, because weather is fine after closing tight crisper cover board
It is bright, the template pouch covered on female cone is directly plucked, polliniferous Brown Glass Brown glass bottles and jars only is filled from crisper taking-up, in left hand
Refer to, nameless and little finger of toe holds vial, thumb and index finger clamp female cone raw small stems keep stablizing, the right hand holds cleaning
Writing brush protrudes into vial, and writing brush head is made to be stained with pollen, and female cone surface is then softly clicked with writing brush head, pollen grain is clicked and entered female
The bract scale mouth opened a business on globe daisy entangles female cone after the bract scale mouth wait open a business is entered pollen with pollination bag from top to bottom, with
It avoids that fitful wind is blown away or pollen on female cone is fallen in rain drop erosion, tightens pollination bag, hang up tag board, write and award on tag board
Powder male parent, female parent and pollination date are extractd after 13 days and cover the pollination bag on female cone.
(6) late October current year Abies ziyuanensis in two populations cone turns yellowish-brown by green, October 23, by artificial pollination
Random acquisition 10 in cone peel seed respectively and carry out dissection and check that the seed for counting artificial pollination cone whether there is or not embryo is full
Full scale.Remaining cone Jing Guo artificial pollination stays in the tree, gives free rein to fall off and sow.To April next year, artificial pollinizers are checked
Peripheral ground fir growth of seedling situation counts Abies ziyuanensis in two populations seedling numbers.
Above three embodiments are done with three plants of different elite stands, and except pollination time is different, remaining operation is phase
With.
Comparative example
Do not implement artificial pollination, allow Abies ziyuanensis in two populations Post flowering spontaneous pollination, steps are as follows:
It (1) is observation object with the Abies ziyuanensis in two populations of Guangxi resource Yinzhulaoshan Abies ziyuanensis in two populations Nature Reserve.Money at the beginning of 4 months
The male cone and female cone of source fir are quickly expanded, and are being provided near male cone plant, and 1 plant of random selection is provided with female cone,
And the Abies ziyuanensis in two populations that the diameter of a cross-section of a tree trunk 1.3 meters above the ground is 33 centimetres, any artificial processing is not done to this plant of Abies ziyuanensis in two populations, by its spontaneous pollination.
(2) late October current year Abies ziyuanensis in two populations cone turns yellowish-brown by green, in October from twenty-first to twenty-second, in step (1)
The upper random acquisition cone 10 of the Abies ziyuanensis in two populations tree chosen peels seed respectively and carries out dissection and check whether there is or not embryo, counts cone
Seed plumpness.Remaining is stayed in the tree without the cone of artificial pollination, is given free rein to fall off and be sowed.To April next year, look into
It sees the Abies ziyuanensis in two populations tree peripheral ground fir growth of seedling situation for not making artificial pollination, counts Abies ziyuanensis in two populations seedling numbers.
Embodiment 1 and comparative example are counted according to the content to be counted in following table, as a result as shown in the table:
| Content | 1 artificial pollination of embodiment | Spontaneous pollination |
| Seed plumpness | 26.4% | 0.01% |
| Tree is lower to grow seedling numbers (strain) | 637 | 0 |
The aforementioned description to specific exemplary embodiment of the invention is in order to illustrate and illustration purpose.These descriptions are not
Want to limit the invention to disclosed precise forms, and it will be apparent that much can be changed and be become according to foregoing description
Change.The purpose for being selected embodiment and being described is to explain the specific principles of the present invention and its practical application, so that
The practitioner of this field can be realized and using a variety of different embodiments of the invention and a variety of different selections and change
Become.
Claims (6)
1. a kind of method that Abies ziyuanensis in two populations artificial pollination promotes Wild population seedlings regeneration, characterized in that comprise the steps of:
(1) pollen collecting: when the male cone of 80%-90% on male cone brachyplast switchs to yellow by yellow green, start to collect pollen；
(2) pollen stores: the pollen of collection being packed into Brown Glass Brown glass bottles and jars only, bottle cap is covered tightly, stores under the conditions of 4 DEG C；
(3) it builds pollination operation rack: building pollination operation rack around the tree crown of Abies ziyuanensis in two populations pollination elite stand；
(4) it pollinates: the 6th~10 day after collecting pollen, pollinating when female cone bract scale is opened a business；
(5) bagging: to put on pollination bag immediately after female cone pollination, and pollination bag is tightened；
(6) remove bag: pollination extractd pollination bag after 10~15 days.
2. the method that Abies ziyuanensis in two populations artificial pollination as described in claim 1 promotes Wild population seedlings regeneration, characterized in that step
Suddenly the method for (1) described pollen collecting are as follows: clip grows the brachyplast of male inflorescence, and insertion is equipped with the bottle of clear water, will be few on brachyplast
The male cone of part or green and yellow green is all extractd, and then water bottle is moved on on a clean blank sheet of paper, allows release
Yellow pollen is trickled down on blank sheet of paper, and the pollen on blank sheet of paper is trickled down in collection.
3. the method that Abies ziyuanensis in two populations artificial pollination as described in claim 1 promotes Wild population seedlings regeneration, characterized in that step
Suddenly (2) described pollen storage is using one or both of following methods:
1) polliniferous Brown Glass Brown glass bottles and jars only will be filled and is put into the refrigerator with dehumidification function, stored under the conditions of 4 DEG C；
2) polliniferous Brown Glass Brown glass bottles and jars only will be filled and is put into plastic seal pocket, the discoloration silica dehydrator of blue is added in sealed bag
Agent is tamping after sack under the conditions of being put into 4 DEG C and stores, when the discoloration silica-gel desiccant water suction of blue becomes light blue or pink
When, the drier self-indicating that more renews in time.
4. the method that Abies ziyuanensis in two populations artificial pollination as described in claim 1 promotes Wild population seedlings regeneration, characterized in that step
Suddenly the diameter of a cross-section of a tree trunk 1.3 meters above the ground of (3) described Abies ziyuanensis in two populations pollination elite stand is greater than 30 centimetres.
5. the method that Abies ziyuanensis in two populations artificial pollination as described in claim 1 promotes Wild population seedlings regeneration, characterized in that step
Suddenly in (4), the method for pollination are as follows: the Brown Glass Brown glass bottles and jars only of storage pollen in step (2) is put into preservation by low temperature box and taken by the pollination same day
It takes under pollination elite stand, is risen by pollination operation rack to by the female cone branch of tree crown top；Clean writing brush is held when pollination to stretch
Enter vial, writing brush head is made to be stained with pollen, female cone surface is then softly clicked with writing brush head, pollen grain is clicked and entered on female cone
The bract scale mouth of the first transaction of a day's business, when the bract scale mouth wait open a business is entered pollen, pollination terminates.
6. the method that Abies ziyuanensis in two populations artificial pollination as described in claim 1 promotes Wild population seedlings regeneration, characterized in that step
Suddenly in (6), pollination 6 months after, the pollination cone of random acquisition maturation peels seed respectively, then dissection seed check whether there is or not
Embryo, the seed plumpness of statistics pollination cone, remaining pollination cone stay in the tree, are allowed to seed and fall to earth's surface naturally
On, check and count the quantity of artificial pollination tree peripheral ground germinating Abies ziyuanensis in two populations seedling April next year.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114303940A (en) * | 2022-01-19 | 2022-04-12 | 广西壮族自治区中国科学院广西植物研究所 | Powder control type dry pollinator for resource fir |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511384A (en) * | 2011-12-31 | 2012-06-27 | 中国林业科学研究院热带林业研究所 | Greenhouse pollination hybridization control method for casuarina |
| CN105210854B (en) * | 2015-10-29 | 2017-07-11 | 山东省林业科学研究院 | A kind of method of Chinese scholar tree artificial pollination |
| CN108243948A (en) * | 2018-02-26 | 2018-07-06 | 山东省林业科学研究院 | A kind of artificial pollination breeding method of salt tolerant fast-growing Fraxinus velutina |
-
2018
- 2018-09-13 CN CN201811070273.8A patent/CN109168874A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511384A (en) * | 2011-12-31 | 2012-06-27 | 中国林业科学研究院热带林业研究所 | Greenhouse pollination hybridization control method for casuarina |
| CN105210854B (en) * | 2015-10-29 | 2017-07-11 | 山东省林业科学研究院 | A kind of method of Chinese scholar tree artificial pollination |
| CN108243948A (en) * | 2018-02-26 | 2018-07-06 | 山东省林业科学研究院 | A kind of artificial pollination breeding method of salt tolerant fast-growing Fraxinus velutina |
Non-Patent Citations (3)
| Title |
|---|
| 佚名: "所党委副书记何茂勋一行赴资源县开展调研", 《广西植物研究所HTTP://WWW.GXIB.CN/CONTENT/?1649.HTML》 * |
| 哀建国: "百山祖冷杉濒危机制与保护对策研究", 《中国优秀硕士学位论文全文数据库（电子期刊）-农业科技辑》 * |
| 蒋桂雄: "《林业新闻看点 2012年广西林业新闻作品选集》", 30 September 2013, 广西科学技术出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114303940A (en) * | 2022-01-19 | 2022-04-12 | 广西壮族自治区中国科学院广西植物研究所 | Powder control type dry pollinator for resource fir |
| US11930751B2 (en) | 2022-01-19 | 2024-03-19 | Guangxi Institute Of Botany, Guangxi Zhuang Autonomous Region And Chinese Academy Of Sciences | Pollen control dry pollination machine for Abies ziyuanensis |
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Application publication date: 20190111 |