CN109136114A - 在丝状真菌宿主细胞中产生多种重组多肽的方法 - Google Patents
在丝状真菌宿主细胞中产生多种重组多肽的方法 Download PDFInfo
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Classifications
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- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
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- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
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- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
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| CN201280052277.8A Pending CN103890180A (zh) | 2011-08-24 | 2012-08-23 | 在丝状真菌宿主细胞中产生多种重组多肽的方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114561303A (zh) * | 2022-02-14 | 2022-05-31 | 大连理工大学 | 一株分泌高性能纤维素酶的里氏木霉工程菌株及其应用 |
| CN117757643A (zh) * | 2024-01-12 | 2024-03-26 | 华东理工大学 | 一种分泌表达人溶菌酶的里氏木霉工程菌及其构建方法和应用 |
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| IN2014CN02136A (enExample) * | 2011-08-24 | 2015-05-29 | Novozymes Inc | |
| US9404101B2 (en) * | 2011-08-24 | 2016-08-02 | Novozymes, Inc. | Methods for obtaining positive transformants of a filamentous fungal host cell |
| BR112014016587A2 (pt) * | 2012-01-05 | 2020-10-27 | Novartis Ag | células fúngicas filamentosas deficientes de protease e métodos de uso das mesmas |
| DK2852610T3 (en) | 2012-05-23 | 2018-09-03 | Glykos Finland Oy | PRODUCTION OF FUCOSYLED GLYCOPROTEIN |
| DK3019602T3 (en) | 2013-07-10 | 2018-11-12 | Glykos Finland Oy | MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF |
| US20150147774A1 (en) * | 2013-11-22 | 2015-05-28 | National University Of Singapore | Expression construct for yeast and a method of using the construct |
| DK3152315T3 (en) * | 2014-06-06 | 2018-11-26 | Novozymes As | ENZYME COMPOSITIONS AND APPLICATIONS THEREOF |
| CA2954974A1 (en) | 2014-07-21 | 2016-01-28 | Glykos Finland Oy | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
| CA2961280C (en) | 2014-09-23 | 2022-08-30 | Liuyang DIAO | Processes for producing ethanol and fermenting organisms |
| US10385352B2 (en) | 2015-03-09 | 2019-08-20 | Novozymes A/S | Methods of introducing multiple expression constructs into a eukaryotic cell |
| US10738293B2 (en) | 2016-03-02 | 2020-08-11 | Novozymes A/S | Cellobiohydrolase variants and polynucleotides encoding same |
| WO2018093752A1 (en) * | 2016-11-15 | 2018-05-24 | Danisco Us Inc. | Filamentous fungi with improved protein production |
| US20190345522A1 (en) | 2016-12-06 | 2019-11-14 | Novozymes A/S | Improved Processes For Production Of Ethanol From Xylose-Containing Cellulosic Substrates Using Engineered Yeast Strains |
| US11091753B2 (en) | 2017-05-31 | 2021-08-17 | Novozymes A/S | Xylose fermenting yeast strains and processes thereof for ethanol production |
| CA3064042A1 (en) | 2017-06-02 | 2018-12-06 | Novozymes A/S | Improved yeast for ethanol production |
| CN109988714B (zh) * | 2017-12-29 | 2021-12-28 | 青岛蔚蓝生物集团有限公司 | 一种里氏木霉及其应用 |
| AU2019213033A1 (en) | 2018-01-29 | 2020-09-17 | Microbiogen Pty. Ltd. | Microorganisms with improved nitrogen utilization for ethanol production |
| BR112021001282A2 (pt) | 2018-07-25 | 2021-04-27 | Novozymes A/S | levedura expressando enzimas para produção de etanol |
| CA3114783A1 (en) | 2018-10-08 | 2020-04-16 | Novozymes A/S | Enzyme-expressing yeast for ethanol production |
| CN113302305A (zh) * | 2018-12-12 | 2021-08-24 | 诺维信公司 | 提高丝状真菌细胞在多肽的产生方面的生产力的方法 |
| CA3143381A1 (en) | 2019-07-26 | 2021-02-04 | Novozymes A/S | Microorganisms with improved nitrogen transport for ethanol production |
| BR112021026477A2 (pt) | 2019-08-06 | 2022-02-08 | Novozymes As | Célula hospedeira recombinante, métodos de produção de um produto de fermentação a partir de um material contendo amido ou contendo celulose, de produção do polipeptídeo maduro, de produção de um derivado de uma célula hospedeira recombinante e de produção de etanol, construto de ácido nucleico ou vetor de expressão, composição, e, uso de uma célula hospedeira recombinante |
| CN110724643B (zh) * | 2019-11-18 | 2022-07-15 | 河南省农业科学院植物保护研究所 | 一株具有防病促生功能的橘灰青霉及其菌剂和应用 |
| EP4073089A1 (en) | 2019-12-10 | 2022-10-19 | Novozymes A/S | Microorganism for improved pentose fermentation |
| EP4208559A1 (en) | 2020-09-04 | 2023-07-12 | Novozymes A/S | Improved fermenting organism for ethanol production |
| AU2022288057A1 (en) | 2021-06-07 | 2023-12-21 | Novozymes A/S | Engineered microorganism for improved ethanol fermentation |
| CN120344672A (zh) | 2022-09-23 | 2025-07-18 | 诺维信公司 | 用于乙醇生产的改进发酵生物 |
| WO2025088155A1 (en) | 2023-10-25 | 2025-05-01 | Novozymes A/S | Improved fermenting organism for ethanol production |
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| WO2010039889A2 (en) * | 2008-09-30 | 2010-04-08 | Novozymes, Inc. | Methods for using positively and negatively selectable genes in a filamentous fungal cell |
| CN101970651A (zh) * | 2007-05-31 | 2011-02-09 | 诺维信股份有限公司 | 用于降解纤维素材料的组合物 |
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| FI864473A7 (fi) * | 1985-11-20 | 1987-05-21 | Panlabs Inc | Yhdistetyt ilmaisukasetit fungaalitransformaatiota varten. |
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| US5989870A (en) | 1986-04-30 | 1999-11-23 | Rohm Enzyme Finland Oy | Method for cloning active promoters |
| US5648263A (en) | 1988-03-24 | 1997-07-15 | Novo Nordisk A/S | Methods for reducing the harshness of a cotton-containing fabric |
| DK115890D0 (da) | 1990-05-09 | 1990-05-09 | Novo Nordisk As | Enzym |
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| PT655890E (pt) * | 1992-07-31 | 2005-05-31 | Ab Enzymes Gmbh | Celulas recombinantes, construcoes de adn, vectores e metodos para expressao de enzimas degradantes de fitato em proporcoes desejadas |
| ATE412048T1 (de) | 1993-03-10 | 2008-11-15 | Novozymes As | Enzyme mit xylanaseaktivität aus aspergillus aculeatus |
| CN1192108C (zh) | 1994-06-03 | 2005-03-09 | 诺沃奇梅兹生物技术有限公司 | 纯化的毁丝霉属漆酶及编码该酶的核酸 |
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| EP0877801B1 (en) | 1996-01-19 | 2005-04-27 | Novozymes Biotech, Inc. | Morphological mutants of filamentous fungi |
| EP0966521A1 (en) * | 1996-09-13 | 1999-12-29 | Novo Nordisk Biotech, Inc. | Cells having dna insertion mutations which produce altered amounts of a polypeptide |
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| US10301610B2 (en) | 2019-05-28 |
| AU2012298799A1 (en) | 2014-02-13 |
| US20170015986A1 (en) | 2017-01-19 |
| CN103890180A (zh) | 2014-06-25 |
| WO2013028912A3 (en) | 2013-06-06 |
| US20140212977A1 (en) | 2014-07-31 |
| WO2013028912A2 (en) | 2013-02-28 |
| US10822601B2 (en) | 2020-11-03 |
| AU2012298799B2 (en) | 2018-02-01 |
| EP2748322A2 (en) | 2014-07-02 |
| US9493790B2 (en) | 2016-11-15 |
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