CN109123236A - A kind of efficient fish mixed feed with anti-oxidation function - Google Patents

A kind of efficient fish mixed feed with anti-oxidation function Download PDF

Info

Publication number
CN109123236A
CN109123236A CN201811118999.4A CN201811118999A CN109123236A CN 109123236 A CN109123236 A CN 109123236A CN 201811118999 A CN201811118999 A CN 201811118999A CN 109123236 A CN109123236 A CN 109123236A
Authority
CN
China
Prior art keywords
feed
fish
lycopene
rubber
vitamin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811118999.4A
Other languages
Chinese (zh)
Inventor
邓君明
张曦
陶琳丽
杨秀娟
毕保良
胡青
孔令富
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yunnan Agricultural University
Original Assignee
Yunnan Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yunnan Agricultural University filed Critical Yunnan Agricultural University
Priority to CN201811118999.4A priority Critical patent/CN109123236A/en
Publication of CN109123236A publication Critical patent/CN109123236A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/116—Heterocyclic compounds
    • A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
    • A23K20/126—Lactones
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/26—Compounds containing phosphorus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The efficient fish mixed feed with anti-oxidation function that the present invention relates to a kind of, belongs to technical field of aquaculture.Replace dregs of beans or fish meal as forage protein source using rubber seedcake part, and add lycopene, the wherein weight percent of various raw materials are as follows: fish meal 39.5~47.8%, rubber seedcake 15~45%, methionine 0.07~0.22%, lysine 0.15~0.47%, leucine 0.07~0.22%, fish oil 0.1~0.6%, soybean oil 0.1~3.1%, soybean lecithin 0.5~1.0%, lycopene 0.01~0.03%, wheat-middlings 10.84~21.46%, alphalise starch 1~8%, vitamin C phosphoric ester 0.02~0.05%, calcium dihydrogen phosphate 0.7~1.2%, salt 0.2~0.3%, choline chloride 0.2~0.3%, vitamin premix 0.5~0.8 %, mineral substance premix 0.8~1.0%；The present invention makes full use of rubber seedcake as forage protein source and n-3 unsaturated fatty acid source of supply, the feedstuff in short supply such as dregs of beans is saved, lycopene is added as natural, the oxidation resistance and immunity of fish is enhanced, improves fish culture benefit.

Description

A kind of efficient fish mixed feed with anti-oxidation function
Technical field
The present invention relates to a kind of fish mixed feeds, and in particular to a kind of efficient fish cooperation feeding with anti-oxidation function Material, belongs to technical field of aquaculture.
Background technique
In recent years, with the improvement of people ' s living standards with the enhancing of health perception, the n-3 system with certain health-care effect Column unsaturated fatty acid increasingly causes the common concern of people, the aquatic products rich in alpha-linolenic acid by numerous consumers blueness It looks at.Currently, China's aquatic products increment mostlys come from culture fishery, current China's aquaculture yield has accounted for total output 72%.With the fast development of culture fishery, the demand of feedstuff is increasing.China is that protein resource is in short supply Country, forage protein source insufficient supply is especially prominent, has 25,000,000 tons or more of protein raw materials notch every year, especially closely Several years, the routine proteins such as dregs of beans, fish meal market price of raw material continuous rise greatly improved feed cost.
Rubber seed is the seed of rubber tree, is a kind of important by-product of ruber plantation.Rubber seed is by shell, benevolence two parts Composition, rubber seed hull account for about the 45% of butt, and rubber seed accounts for about 55% (oil-containing 45%~52% in seed benevolence) of butt.Cause This, rubber seed can be divided into three parts after processing: rubber seed oil, rubber seedcake, rubber seed hull.Under normal circumstances, rubber seedcake contains (unsaturated fatty acid content is sub- oily up to 82% for crude protein 25~30% (essential amino acid balanced proportion), crude fat 12~16% Acid and alpha-linolenic acid are up to 60% or more), rich in mineral elements (being shown in Table 1) such as calcium phosphorus.It is formed in rubber seedcake/oil fatty acid In, it is 3-4 times of soybean oil and rapeseed oil that n-3 series unsaturated fatty acid (alpha-linolenic acid) content, which is up to 18-20%,；N-6 system Column unsaturated fatty acid (linoleic acid) content is 34-38%, and n-3/n-6 ratio is 1/2, is a kind of typically rich in n-3 insatiable hunger With the plant feed raw material of fatty acid.Rubber seed is that a kind of larger stock number, cultivated area and yield are only second to walnut in China With the woody oleiferous plants of tea seed.About 930,000 tons of China's rubber seed annual output in 2013, about 700,000 tons of Yunnan Province's rubber seed annual output； Meanwhile the resource in the whole world 2/3rds has been concentrated in the Lancang Mekong River basin where Yunnan Province, produces rubber seed 6,400,000 per year Ton.Therefore, rubber seedcake can be used as routine protein raw material substitute and a kind of potential n-3 unsaturated fatty acid Source of supply has very high nutritive value, it may be considered that as the substitute of the protein raw materials such as dregs of beans, fish meal.
Table 1, rubber seedcake conventional nutrient composition
In the prior art, patent CN103284018A, CN103300222A, CN103300223A, CN103300255A, CN103300256A relates to the use of protein raw materials of the rubber seedcake as fish feed；Patent CN104719637A, CN104719678A, CN104719679A relate to the use of glyceride stock of the rubber seed oil as fish feed.However, rubber seed Unsaturated fatty acid is rich in cake/oil, high-content rubber seedcake/oil intake easily leads to grease/feed oxide and goes bad, generation aldehyde, The harmful substances such as ketone.In addition, oxidation grease/feed feeds fish, fish growth can be hindered；Cause muscular atrophy, muscle fibre row Column are chaotic, and transparent and pellet degradation occurs；The mobility of biomembrane is reduced, and then destroys the integrality of membrane structure；Draw Play liver enlargement and necrosis of liver cells.Therefore, generally in grease/feed add chemical antioxidants (such as ethoxyquinoline) with Prevent or slow down the oxidation reaction of grease.However, research confirms that the chemical antioxidants such as ethoxyquinoline have one to fish immunity Determine side effect, the feed containing ethoxyquinoline, which is used for a long time, can be such that fish body premunition declines.In addition, ethoxyquinoline can pass through Feed enters in animal body, and metabolite-ethoxyquinoline quinone has genetoxic.Therefore, on 2 6th, 2017, committee of European Union Member can notify, and implementing regulations draft is paused at and uses ethoxyquinoline in all animal feed additives.Therefore, it finds Safe and reliable natural has a very important significance.
Summary of the invention
In view of the above-mentioned problems, the present invention provides a kind of efficient fish mixed feed with anti-oxidation function, the present invention In a reasonable range, by-product-rubber seedcake part in ruber plantation is made full use of to replace dregs of beans or fish meal, both Dregs of beans or this forage protein source in short supply of fish meal have been saved, fish mixed feed cost is reduced；N-3 abundant is provided again not Saturated fatty acid meets fish to the needs of the essential fatty acids such as alpha-linolenic acid, improves aquatic products quality；Meanwhile it adding Natural-lycopene is configured to a kind of fish mixed feed with anti-oxidation function, overcome rubber seedcake this The shortcomings that one high-quality feed protein sources, sufficiently releases lycopene biological antioxidant activity, also enhances the anti-oxidant of fish Ability and immunity, and then improve feed cost performance and fish culture benefit.
To achieve the goals above, the technical scheme adopted by the invention is that:
Replace dregs of beans or fish meal as forage protein source using rubber seedcake part, and adds tomato red in feed formula Element.
A kind of efficient fish mixed feed with anti-oxidation function, including dregs of beans, rubber seedcake, methionine, lysine, Histidine, soybean oil, soybean lecithin, lycopene, wheat-middlings, grass meal, alphalise starch, calcium dihydrogen phosphate, vitamin C phosphoric ester, Salt, choline chloride, vitamin premix, mineral substance premix, wherein the weight percent of various raw materials are as follows: dregs of beans 38~ 47%, rubber seedcake 9~36%, methionine 0.5~0.6%, lysine 0.4~0.7%, histidine 0.2~0.3%, soybean Oil 0.1~6%, soybean lecithin 0.5~1.0%, lycopene 0.01~0.03%, wheat-middlings 18~35%, grass meal 3~5%, Alphalise starch 0~3%, calcium dihydrogen phosphate 0.7~1.2%, vitamin C phosphoric ester 0.02~0.05%, salt 0.2~0.3%, chlorine Change choline 0.2~0.3%, vitamin premix 0.5~0.8%, mineral substance premix 0.8~1.0%；The weight of all raw materials The sum of percentage is 100%.
Lycopene (Lycopene) is also known as ψ-carrotene, belongs to isoprenoid compound, is natural It is a kind of.
Lycopene is by physics and chemical mode quenching singlet oxygen or captures peroxylradicals, and lycopene can also Eliminating oxyradical by the chemical reaction of the active oxygen with other forms, (such as hydrogen peroxide, nitrite anions oxidation is free Base).The effect of lycopene removing free radical, outclass other carotenoid and vitamin E, and singlet-oxygen quenching rate is normal Number is 100 times of vitamin E.Currently, lycopene is most one of the powerful antioxidant being found in natural plant, can apply In feed addictive.
Lycopene is a kind of liposoluble substance, needs to be dissolved in base oil, and quality quality is directly related to tomato The effect of red pigment, plays and total quality.
Lycopene can effectively remove the intracorporal free radical of animal, keep cell eubolism, improve the antioxygen of animal Change ability and immunity of organisms.
Beneficial effects of the present invention:
(1) by-product-rubber seedcake in ruber plantation is made full use of, as the protein raw materials in fish mixed feed Part dregs of beans or fish meal are substituted, dregs of beans or fish meal this protein raw materials in short supply has been saved, has reduced fish feed cost；
(2) it is also rich in n-3 unsaturated fatty acid in rubber seedcake, had both met fish to essential fatty acids such as alpha-linolenic acids Needs, and improve aquatic products quality；
(3) lycopene is added in mixed feed as natural, both overcomes this high-quality feeding of rubber seedcake The shortcomings that expecting protein sources, and sufficiently release lycopene biological antioxidant activity, also enhance the oxidation resistances of fish with Immunity, and then improve feed cost performance and fish culture benefit.
Specific embodiment
Below in conjunction with specific embodiment, the technical solution in the present invention is clearly and completely described, it is clear that institute The embodiment of description is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, Every other embodiment obtained by those of ordinary skill in the art without making creative efforts, belongs to this hair The range of bright protection.
Embodiment one
Using dregs of beans and rubber seedcake as forage protein source, soybean oil, soybean lecithin and lycopene/ethoxyquinoline The mixed feed ready-mixed oil of preparation has prepared the energy such as 6 kinds of equal nitrogen feed (such as according to conventional feed mix proportion scheme as fat source Shown in table 2), wherein it is not added with rubber seedcake in 1 feed of example, as a control group；Rubber seedcake is added in 2~5 feed of example Amount, with Substitution for Soybean Meal, is configured to the mixed feed of the unsaturated fatty acid of n-3/n-6 containing different proportion for 9~36%.Example 1~5 Lycopene is added in feed as antioxidant, using ethoxyquinoline as antioxidant in 6 feed of example.
Table 2, each example feed formula (%, dry matter basis)
Note: mineral substance premix (g/kg): MgSO4·7H2O, 180；KI, 1；FeSO4·H2O, 260；ZnSO4·H2O, 180；CuSO4·5H2O, 25；Na2Se2O3, 0.01；MnSO4·H2O, 180；CoCl2·6H2O, 0.75；Vitamin premix (g/ Kg): vitamin A, 2；Vitamin D3, 0.03；Vitamin E, 30；Vitamin K, 3；Thiamine hydrochloride, 8；Riboflavin, 11；Hydrochloric acid pyrrole It trembles alcohol, 8；Vitamin B12, 0.02；Ascorbic acid, 50；Folic acid, 1；Biotin, 0.1；Niacin, 30；Calcium pantothenate, 32；Inositol, 25.
All feedstuffs are crushed by pulverizer, and all cross 60 meshes, by all feedstuffs crushed by table 2 Feed formula is by other in addition to the feed ready-mixed oil that soybean oil, soybean lecithin and lycopene/ethoxyquinoline are prepared Raw material is uniformly mixed, and soybean oil, soybean lecithin and lycopene/ethoxyquinoline (soybean lecithin and tomato is then added Red pigment/ethoxyquinoline is first dissolved in soybean oil), it is uniformly mixed, finally adding about 30% distilled water makes mash feed shape It at hard group, is placed in pelleter and feed is squeezed into the strip of diameter 1.0mm, and drying 12 is small in 40 DEG C of convection ovens When, so that its moisture content is down to 12% or less.Through detecting, the nutritional ingredient of each example feed formula of the invention is as shown in table 3.
Table 3, each example feed nutrient
Test is the same a collection of seed of artificial culture with grass carp, is provided by certain local farm.Before test, grass carp first uses quotient Industry feed supports 2 weeks temporarily to adapt to breeding environment.Grass carp fasting is for 24 hours before on-test.Select (about 5.8g) uniform in size, physique strong Strong grass carp is randomly assigned in 18 breeding barrels (6 test process groups, every group of 3 repetitions), every barrel of 40 tails.It throws sooner or later daily Bait 2 times (07:00,18:00), up to the level of being satiated with food.Breeding water is the tap water of aeration dechlorination.Progress 10 weeks altogether are tested, entirely It is all made of continuous oxygenation, natural lighting, water temperature for 24 hours during culture experiment and remains at 22~26 DEG C.
After culture experiment, stop feeding for 24 hours, weigh every barrel of fish body gross weight and record fish body number, it is raw to calculate grass carp Long rate and survival rate.In addition, the tissue samples such as acquisition Blood of Ctenopharyngodon, enteron aisle and liver, and analyze measurement digestive ferment, resistance to oxidation With the indexs such as immunity.The analysis test of each index is all made of maturation method used in research both at home and abroad.All equal tables of numerical value It is shown as
ˉ
Average value ± standard error (x ± SE), it is significant when existing between processing group using one-way analysis of variance (ANOVA) When sex differernce (P < 0.05), Multiple range test analysis is carried out with Tukey ' s inspection.Comparative analysis difference antioxidant is examined using T Group difference.
Table 4~6 is test data and test result.
Table 4, each example Growth of Grass Carps Ctenopharyngodon Idellus results of property
Note: colleague's shoulder infuses different letter persons, significant difference (P < 0.05)；Indicate that T examines difference between example 3 and 6 groups of example Significantly.
As shown in Table 4: with the raising of rubber seedcake Substitution for Soybean Meal ratio in feed, grass carp end weight and increases day by day at body weight gains Weight coefficient is in the trend being gradually reduced；The grass carp fed using 1~4 feed of example, feed coefficient and protein efficiency ratio, PER are equal There was no significant difference.36% rubber seedcake is added in feed and significantly reduces Growth of Grass Carps Ctenopharyngodon Idellus performance, but adds 9~27% pairs of feeds Utilization rate is without obvious negative effect.
Again 1~5 feed of comparison example feeding grass carp data, it can be found that 2 feed feeding group feed coefficient of example and Protein efficiency ratio, PER is substantially better than other example feed feeding groups.(the n-3:n-6 unsaturated lipid when rubber seedcake additive amount is 9% Fat acid=1:4.28), effect is best.
In addition, as shown in Table 4, the grass carp fed using 3 feed of example, end weight, body weight gains and daily gain coefficient are aobvious It writes and is higher than 6 feed feeding group of example.Antioxidant of the lycopene as fish mixed feed improves Growth of Grass Carps Ctenopharyngodon Idellus performance effect Fruit is substantially better than ethoxyquinoline.
Table 5, each example grass carp pipe intestinal digesting enzymatic activity result
Note: colleague's shoulder infuses different letter persons, significant difference (P < 0.05)；Indicate that T examines difference between example 3 and 6 groups of example Significantly.
As shown in Table 5: with the raising of rubber seedcake Substitution for Soybean Meal ratio in feed, grass carp intestinal trypsase and starch Enzymatic activity is in downward trend after first rising；Wherein, the grass carp fed using 2 feed of example, enteron aisle trypsase and shallow lake Powder enzymatic activity highest is significantly higher than 5 feed feeding group of example.With the raising of rubber seedcake Substitution for Soybean Meal ratio in feed, grass Fish intestinal fat enzyme and Disacchridase Activities are in the trend being gradually reduced；Wherein, the grass carp fed using 1~2 feed of example, There was no significant difference for intestinal fat enzyme and Disacchridase Activities.9~18% rubber seedcakes are added in feed to grass carp intestinal digestive ferment Activity is without obvious negative effect；But when additive amount reaches 36%, grass carp intestinal digestive enzyme activity is obviously inhibited.
The grass carp data of 1~5 feed of comparison example feeding again, it can be found that 2 feed feeding group grass carp intestinal pancreas of example Protease, lipase and amylase activity are apparently higher than other embodiments feed feeding group.When rubber seedcake additive amount is 9% (n-3:n-6 unsaturated fatty acid=1:4.28), effect is best.
In addition, as shown in Table 5, the grass carp fed using 3 feed of example, enteron aisle trypsase and lipase active are significant Higher than 6 feed feeding group of example.Antioxidant of the lycopene as fish mixed feed improves grass carp intestinal digestive enzyme Property effect is substantially better than ethoxyquinoline.
Table 6, each example Liver of Ctenopharyngodon Idellus oxidation resistance index result
Note: colleague's shoulder infuses different letter persons, significant difference (P < 0.05)；Indicate that T examines difference between example 3 and 6 groups of example Significantly.
As shown in Table 6: with the raising of rubber seedcake Substitution for Soybean Meal ratio in feed, Liver of Ctenopharyngodon Idellus catalase and mistake Oxide enzyme is in downward trend after first rising；Wherein, the grass carp fed using 2 feed of example, liver hydrogen peroxide enzyme activity Property is significantly higher than other embodiments feed feeding group；The grass carp fed using 2 feed of example, liver peroxidase are significantly high In 5 feed feeding group of example.With the raising of rubber seedcake Substitution for Soybean Meal ratio in feed, Liver of Ctenopharyngodon Idellus glutathione reduction Enzyme, antioxidative activities and alkaline phosphatase activities are in the trend being gradually reduced；Wherein, the grass fed using 1~3 feed of example Fish, liver glutathione reductase and antioxidative activities activity are significantly higher than 4~5 feed feeding group of example；It is raised using example 5 Expect the grass carp of feeding, liver alkaline phosphatase activities are substantially less than 1 feed feeding group of example.On the contrary, with rubber in feed The raising of seedcake Substitution for Soybean Meal ratio, Liver of Ctenopharyngodon Idellus activity of glutathione peroxidase are in the trend risen after falling before；Its In, the grass carp fed using 5 feed of example, liver activity of glutathione peroxidase is significantly higher than the feeding of 1 feed of example Group.9~18% rubber seedcakes are added in feed to grass carp resistance to oxidation without obvious negative effect, but add 27~36% rubber seeds Cake significantly reduces grass carp oxidation resistance.
The grass carp data of 1~5 feed of comparison example feeding again, it can be found that 2 feed feeding group Liver of Ctenopharyngodon Idellus mistake of example Hydrogen oxide enzyme and peroxidase activity are apparently higher than other example feed feeding groups.(the n- when rubber seedcake additive amount is 9% 3:n-6 unsaturated fatty acid=1:4.28), effect is best.
In addition, as shown in Table 6, the grass carp fed using 3 feed of example, liver catalase, peroxidase and Antioxidative activities activity is significantly higher than 6 feed feeding group of example.Antioxidant of the lycopene as fish mixed feed, changes Kind grass carp oxidation resistance effect is substantially better than ethoxyquinoline.
In conclusion adding 9~18% rubber seedcakes in grass carp compound feed to efficiency of feed utilization, grass carp intestinal digestive ferment Activity and resistance to oxidation are without obvious adverse effect；And when additive amount is 9%, efficiency of feed utilization, grass carp intestinal digestive enzyme activity It is improved with oxidation resistance；But when additive amount reaches 27~36%, Growth of Grass Carps Ctenopharyngodon Idellus performance, efficiency of feed utilization, enteron aisle disappear Change enzymatic activity and resistance to oxidation is decreased obviously.Therefore, when rubber seedcake additive amount is 9~18% in grass carp compound feed, for most Good proportion, n-3:n-6 unsaturated fatty acid ratio is 1:3.06~4.28 in mixed feed at this time, both will not be to grass carp digestive ferment Activity, efficiency of feed utilization and oxidation resistance generate adverse effect, and the optimal of aquatic products quality and culture efficiency may be implemented Change.
Present invention combination grass carp physiological property for Growth of Grass Carps Ctenopharyngodon Idellus performance, efficiency of feed utilization, Activity of Digestive Enzymes in Intestine and resists Oxidizing force is designed, and replaces dregs of beans using the by-product in ruber plantation-rubber seedcake part, both saved dregs of beans this Forage protein source in short supply, reduces grass carp compound feed cost；N-3 unsaturated fatty acid abundant is provided again, meets grass Fish improves aquatic products quality to the needs of the essential fatty acids such as alpha-linolenic acid；Meanwhile adding natural-tomato red Element is configured to a kind of grass carp compound feed with anti-oxidation function, overcomes lacking for this high-quality feed protein sources of rubber seedcake Point sufficiently releases the biological antioxidant activity of lycopene, also enhances the oxidation resistance and immunity of grass carp, Jin Erti High feed cost performance and grass carp culture efficiency.
Embodiment two
Using fish meal and rubber seedcake as forage protein source, fish oil, soybean lecithin and lycopene/ethoxyquinoline are matched The mixed feed ready-mixed oil of system is as fat source, and according to conventional feed mix proportion scheme, having prepared 6 kinds of equal nitrogen etc. can feed (such as table 7 It is shown), wherein it is not added with rubber seedcake in 1 feed of example, as a control group；Rubber seedcake additive amount in 2~5 feed of example For 15~60% to substitute fish meal, it is configured to the mixed feed of the unsaturated fatty acid of n-3/n-6 containing different proportion.Example 1~5 is raised Lycopene is added in material as antioxidant, using ethoxyquinoline as antioxidant in 6 feed of example.
Table 7, each example feed formula (% dry matter basis)
Note: mineral substance premix (g/kg): MgSO4·7H2O, 180；KI, 1；FeSO4·H2O, 260；ZnSO4·H2O, 180；CuSO4·5H2O, 25；Na2Se2O3, 0.01；MnSO4·H2O, 180；CoCl2·6H2O, 0.75；Vitamin premix (g/ Kg): vitamin A, 2；Vitamin D3, 0.03；Vitamin E, 30；Vitamin K, 3；Thiamine hydrochloride, 8；Riboflavin, 11；Hydrochloric acid pyrrole It trembles alcohol, 8；Vitamin B12, 0.02；Ascorbic acid, 50；Folic acid, 1；Biotin, 0.1；Niacin, 30；Calcium pantothenate, 32；Inositol, 25.
All feedstuffs are crushed by pulverizer, and all cross 60 meshes, by all feedstuffs crushed by table 7 Feed formula by addition to the feed ready-mixed oil that fish oil, soybean lecithin and lycopene/ethoxyquinoline are prepared other are former Material is uniformly mixed, be then added fish oil, soybean lecithin and lycopene/ethoxyquinoline (soybean lecithin and lycopene/ Ethoxyquinoline is first dissolved in fish oil), it is uniformly mixed, finally adding about 30% distilled water makes mash feed form hard group, It is placed in pelleter and feed is squeezed into the strip of diameter 3.0mm, and is 12 hours dry in 40 DEG C of convection ovens, make it Moisture content is down to 12% or less.Through detecting, the nutritional ingredient of each example feed formula of the invention is as shown in table 8.
Table 8, each example feed nutrient
Test is the same a collection of seed of artificial culture with Tilapia mossambica, is provided by certain local farm.Before test, Tilapia mossambica is first Support 2 weeks temporarily with commercial feed to adapt to breeding environment.Tilapia mossambica fasting is for 24 hours before on-test.Select it is uniform in size (about 65.3g), physically fit Tilapia mossambica is randomly assigned in 18 breeding barrels (6 test process groups, every group of 3 repetitions), every barrel 30 tails.Daily morning and evening bait throwing in 2 times (07:00,18:00), up to the level of being satiated with food.Breeding water is the tap water of aeration dechlorination.Test It carries out 10 weeks altogether, continuous oxygenation, natural lighting, water temperature for 24 hours is all made of during entire culture experiment and remains at 27~29 DEG C.
After culture experiment, stop feeding for 24 hours, weigh every barrel of fish body gross weight and record fish body number, calculates Tilapia mossambica Growth rate and survival rate.In addition, the tissue samples such as acquisition Tilapia mossambica blood, enteron aisle and liver, and analyze measurement digestive ferment, antioxygen Change the indexs such as power and immunity.The analysis test of each index is all made of maturation method used in research both at home and abroad.All numerical value It is represented as average value ± standard errorIt is aobvious when existing between processing group using one-way analysis of variance (ANOVA) When writing sex differernce (P < 0.05), Multiple range test analysis is carried out with Tukey ' s inspection.Examine comparative analysis difference anti-oxidant using T Agent group difference.
Table 9~11 is test data and test result.
Table 9, each example Growth Op Tilapia results of property
Note: colleague's shoulder infuses different letter persons, significant difference (P < 0.05)；Indicate that T examines difference between example 3 and 6 groups of example Significantly.
As shown in Table 9: with the raising of rubber seedcake substitution fish meal ratio in feed, Tilapia mossambica end weight, body weight gains and day The coefficient that increases weight is in the trend being gradually reduced；The Tilapia mossambica fed using 1~4 feed of example, there was no significant difference for end weight；Make The Tilapia mossambica fed with 1~2 feed of example, body weight gains and daily gain coefficient there are no significant difference；It is raised using example 1~4 Expect the Tilapia mossambica of feeding, feed efficiency and protein efficiency ratio, PER there are no significant difference.15~45% rubber seeds are added in feed Cake is to Growth Op Tilapia performance and efficiency of feed utilization without obvious negative effect.
In addition, as shown in Table 9, the Tilapia mossambica fed using 3 feed of example, end weight, body weight gains and daily gain coefficient are equal It is significantly higher than 6 feed feeding group of example；Conversely, 3 feed feeding group feed coefficient of example is significantly lower than 6 feed feeding group of example. Antioxidant of the lycopene as fish mixed feed improves the Growth Op Tilapia impact of performance and is substantially better than ethoxyquin Quinoline.
Table 10, each example Tilapia mossambica Activity of Digestive Enzymes in Intestine result
Note: colleague's shoulder infuses different letter persons, significant difference (P < 0.05)；Indicate that T examines difference between example 3 and 6 groups of example Significantly.
As shown in Table 10: with the raising of rubber seedcake substitution fish meal ratio in feed, Tilapia mossambica intestinal protein enzymatic activity In downward trend after first rising；The Tilapia mossambica fed using 2 feed of example, enteron aisle tryptic activity are significantly higher than example 4~5 feed feeding groups.With the raising of rubber seedcake substitution fish meal ratio in feed, Tilapia mossambica intestinal fat enzyme and disaccharidase Activity is in the trend being gradually reduced；The Tilapia mossambica fed using 1 feed of example, intestinal fat enzyme and Disacchridase Activities are significantly high In 4~5 feed feeding group of example.It can be seen that: 15~30% rubber seedcakes are added in feed to Tilapia mossambica enteron aisle digestive enzyme Property is without obvious negative effect；But when additive amount reaches 45~60%, Tilapia mossambica Activity of Digestive Enzymes in Intestine is inhibited by certain.
The Tilapia mossambica data of 1~5 feed of comparison example feeding again, it can be found that 2 feed feeding group Tilapia mossambica intestines of example Road tryptic activity is substantially better than other embodiments feed feeding group.When rubber seedcake additive amount is 15%, (n-3:n-6 is not Saturated fatty acid=1:0.52), effect is best.
In addition, as shown in Table 10, using the Tilapia mossambica of 3 feed of example feeding, enteron aisle trypsase and lipase active It is significantly higher than 6 feed feeding group of example.Antioxidant of the lycopene as fish mixed feed improves Tilapia mossambica enteron aisle and disappears Change enzymatic activity effect and is substantially better than ethoxyquinoline.
Table 11, each example Tilapia mossambica blood plasma and liver oxidation resistance index result
Note: colleague's shoulder infuses different letter persons, significant difference (P < 0.05)；Indicate that T examines difference between example 3 and 6 groups of example Significantly.
As shown in Table 11: with the raising of rubber seedcake substitution fish meal ratio in feed, Tilapia mossambica blood plasma catalase Activity and mda content are in the trend being gradually reduced；Wherein, the Tilapia mossambica fed using 5 feed of example, blood plasma peroxidating Hydrogenase activity is substantially less than 1 feed feeding group of example；The Tilapia mossambica fed using the feed of example 2 and 4, plasma malonaldehyde content Substantially less than 1 feed feeding group of example；Using 1~5 feed of example feed Tilapia mossambica, blood plasma lysozyme, alkaline phosphatase, Peroxidase, glutathione peroxidase and antioxidative activities activity there are no significant difference.
As shown in Table 11: with the raising of rubber seedcake substitution fish meal ratio in feed, liver of hybrid tilapia glutathione mistake Peroxidase activity is in the trend being gradually reduced；Wherein, the Tilapia mossambica fed using 1~2 feed of example, liver glutathione Peroxidase activity is significantly higher than 5 feed feeding group of example；The Tilapia mossambica fed using 1~5 feed of example, liver peroxide Change hydrogen enzyme, peroxidase and antioxidative activities activity and mda content there are no significant difference.In feed addition 15~ 45% rubber seedcake is to Tilapia mossambica resistance to oxidation and immunity without obvious negative effect.
The Tilapia mossambica data of 1~5 feed of comparison example feeding again, it can be found that 2 feed feeding group Tilapia mossambica blood of example It starches mda content and is significantly lower than 1 feed feeding group of example, and liver activity of glutathione peroxidase is slightly above example 1 Feed feeding group.When rubber seedcake additive amount is 15% (n-3:n-6 unsaturated fatty acid=1:0.52), effect is best.
In addition, as shown in Table 11, the Tilapia mossambica fed using 3 feed of example, peroxide plasma enzyme, catalase It is significantly higher than 6 feed feeding group of example with antioxidative activities activity and CAT activity of liver；Conversely, 3 feed of example is raised It feeds group blood plasma and liver mda content is substantially less than 6 feed feeding group of example.Lycopene is as the anti-of fish mixed feed Oxidant improves Tilapia mossambica oxidation resistance effect and is substantially better than ethoxyquinoline.
In conclusion adding 15~45% rubber seedcakes in compound feed of tilapia to Growth Op Tilapia performance, feed benefit With rate, resistance to oxidation and immunity index without obvious adverse effect；And when additive amount is 15%, Tilapia mossambica enteron aisle trypsase Activity and resistance to oxidation index are improved；But when additive amount reaches 60%, Growth Op Tilapia performance, efficiency of feed utilization and anti- Oxidizing force is decreased obviously.Therefore, rubber seedcake broiler diets are 15~45% in compound feed of tilapia, n- in mixed feed 3:n-6 unsaturated fatty acid ratio is 1:0.52~1.00.
Embodiment three
Using fish meal and rubber seedcake as forage protein source, fish oil, soybean oil, soybean lecithin and lycopene/ethoxy The mixed feed ready-mixed oil that base quinoline is prepared has prepared 5 kinds and has waited nitrogen etc. that can raise as fat source according to conventional feed mix proportion scheme Expect (as shown in table 12), wherein be not added with rubber seedcake in 1 feed of example, as a control group；Rubber seed in 2~4 feed of example Cake additive amount is 15~45% to substitute fish meal, is configured to the mixed feed of the unsaturated fatty acid of n-3/n-6 containing different proportion；It is real Lycopene is added in 1~4 feed of example as antioxidant, using ethoxyquinoline as antioxidant in 5 feed of example.
Table 12, each example feed formula (%, dry matter basis)
Note: mineral substance premix (g/kg): MgSO4·7H2O, 180；KI, 1；FeSO4·H2O, 260；ZnSO4·H2O, 180；CuSO4·5H2O, 25；Na2Se2O3, 0.01；MnSO4·H2O, 180；CoCl2·6H2O, 0.75；Vitamin premix (g/ Kg): vitamin A, 2；Vitamin D3, 0.03；Vitamin E, 30；Vitamin K, 3；Thiamine hydrochloride, 8；Riboflavin, 11；Hydrochloric acid pyrrole It trembles alcohol, 8；Vitamin B12, 0.02；Ascorbic acid, 50；Folic acid, 1；Biotin, 0.1；Niacin, 30；Calcium pantothenate, 32；Inositol, 25.
All feedstuffs are crushed by pulverizer, and all cross 60 meshes, by all feedstuffs crushed by table 12 feed formulas will be in addition to the feed ready-mixed oil that soybean oil, fish oil, soybean lecithin and lycopene/ethoxyquinoline are prepared Other raw materials be uniformly mixed, soybean oil, fish oil, soybean lecithin and lycopene/ethoxyquinoline (soybean ovum is then added Phosphatide and lycopene/ethoxyquinoline are first dissolved in soybean oil/fish oil), it is uniformly mixed, finally adds about 30% distillation Water makes mash feed form hard group, is placed in pelleter and feed is squeezed into the strip of diameter 1.0mm, and in 40 DEG C of air blast It is 12 hours dry in baking oven, so that its moisture content is down to 12% or less.Through detecting, the nutrition of each example feed formula of the invention Ingredient is as shown in table 13.
Table 13, each example feed nutrient
Test is the same a collection of seed of artificial culture with mystus nemurus, is provided by certain local farm.Before test, mystus nemurus is first Support 2 weeks temporarily with commercial feed to adapt to breeding environment.Mystus nemurus fasting is for 24 hours before on-test.Select (about 1.2g) uniform in size, Physically fit mystus nemurus is randomly assigned in 15 breeding barrels (5 test process groups, every group of 3 repetitions), every barrel of 40 tails.Often Its morning and evening bait throwing in 2 times (07:00,18:00), up to the level of being satiated with food.Breeding water is the tap water of aeration dechlorination.Test carries out 8 altogether Week it is all made of continuous oxygenation, natural lighting, water temperature for 24 hours during entire culture experiment and remains at 27~29 DEG C.
After culture experiment, stop feeding for 24 hours, weigh every barrel of fish body gross weight and record fish body number, calculates mystus nemurus Growth rate and survival rate.In addition, the tissue samples such as acquisition mystus nemurus blood, enteron aisle and liver, and analyze measurement digestive ferment, antioxygen Change the indexs such as power and immunity.The analysis test of each index is all made of maturation method used in research both at home and abroad.All numerical value It is represented as average value ± standard errorIt is aobvious when existing between processing group using one-way analysis of variance (ANOVA) When writing sex differernce (P < 0.05), Multiple range test analysis is carried out with Tukey ' s inspection.Examine comparative analysis difference anti-oxidant using T Agent group difference.
Table 14~17 is test data and test result.
Table 14, each example mystus nemurus growth performance result
Note: colleague's shoulder infuses different letter persons, significant difference (P < 0.05)；Indicate that T examines difference between example 2 and 5 groups of example Significantly.
As shown in Table 14: with the raising of rubber seedcake substitution fish meal ratio in feed, mystus nemurus end weight, body weight gains, day Weight gain coefficient and protein efficiency ratio, PER are in downward trend after first rising；The mystus nemurus fed using 1~3 feed of example, end Weight, body weight gains, daily gain coefficient and protein efficiency ratio, PER there are no significant difference；The mystus nemurus fed using 1~4 feed of example, There was no significant difference for its feed coefficient.15~30% rubber seedcakes are added in feed to mystus nemurus growth performance and efficiency of feed utilization Without obvious negative effect.
The mystus nemurus data of 1~4 feed of comparison example feeding again, it can be found that 2 feed feeding group mystus nemurus end of example Weight, body weight gains, daily gain coefficient, feed coefficient and protein efficiency ratio, PER are substantially better than other example feed feeding groups.Work as rubber seed When cake additive amount is 15% (n-3:n-6 unsaturated fatty acid=1.15:1), effect is best.
In addition, as shown in Table 14, the mystus nemurus fed using 2 feed of example, end weight, body weight gains and daily gain coefficient are equal It is significantly higher than 5 feed feeding group of example；Conversely, 2 feed feeding group feed coefficient of example is significantly lower than 5 feed feeding group of example. Antioxidant of the lycopene as fish mixed feed improves mystus nemurus growth performance effect and is substantially better than ethoxyquin Quinoline.
Table 15, embodiment mystus nemurus pipe intestinal digesting enzymatic activity result
Note: colleague's shoulder infuses different letter persons, significant difference (P < 0.05)；Indicate that T examines difference between example 2 and 5 groups of example Significantly.
As shown in Table 15: the mystus nemurus fed using 1~4 feed of example, pepsin and intestinal fat enzymatic activity are equal There was no significant difference；The mystus nemurus fed using 1~2 feed of example, enteron aisle trypsase and amylase activity are also without significant Sex differernce；The mystus nemurus fed using 3~4 feed of example, enteron aisle tryptic activity are substantially less than the feeding of 1 feed of example Group；However, the mystus nemurus fed using 4 feed of example, Amylase Activity are significantly higher than 3 feed feeding group of example.It raises 15% rubber seedcake is added in material to mystus nemurus pipe intestinal digesting enzymatic activity without obvious negative effect；But when additive amount reaches 30~ When 45%, mystus nemurus Activity of Digestive Enzymes in Intestine is inhibited by certain.
In addition, as shown in Table 15, using the mystus nemurus of 2 feed of example feeding, enteron aisle trypsase and lipase active It is significantly higher than 5 feed feeding group of example.Antioxidant of the lycopene as fish mixed feed improves mystus nemurus enteron aisle and disappears Change enzymatic activity effect and is substantially better than ethoxyquinoline.
Table 16, each example mystus nemurus blood plasma and liver oxidation resistance index result
Note: colleague's shoulder infuses different letter persons, significant difference (P < 0.05)；Indicate that T examines difference between example 2 and 5 groups of example Significantly.
As shown in Table 16: with the raising of rubber seedcake substitution fish meal ratio in feed, mystus nemurus plasma malonaldehyde content In the trend risen after falling before；Wherein, the mystus nemurus fed using 4 feed of example, plasma malonaldehyde content are significantly higher than 3 feed feeding group of example；The mystus nemurus fed using 1 feed of example, blood plasma IgM level are substantially less than the feeding of 2~4 feed of example Feed group；The mystus nemurus fed using 1~4 feed of example, there was no significant difference for blood plasma alkaline phosphatase activities.
As shown in Table 16: the mystus nemurus fed using 1~3 feed of example, CAT activity of liver are significantly higher than 4 feed feeding group of example；Using 3 feed of example feed mystus nemurus, liver lactic acid dehydrogenase activity be significantly higher than example 1~ 2 feed feeding groups；The mystus nemurus fed using 1 feed of example, liver content of nitric oxide is significantly higher than example 2,4 feeds are raised Feed group；The mystus nemurus fed using 1~4 feed of example, Liver Superoxide Dismutase Activity, glutathione peroxidase, paddy The sweet fabk polypeptide of Guang, peroxidase and alkaline phosphatase activities there are no significant difference.15~30% rubber are added in feed Seedcake is to mystus nemurus resistance to oxidation and immunity without obvious negative effect.
The mystus nemurus data of 1~4 feed of comparison example feeding again, it can be found that 2~3 feed feeding group mystus nemurus of example Plasma malonaldehyde content is significantly lower than 1 feed feeding group of example, and blood plasma IgM content is apparently higher than 1 feed feeding group of example.When When rubber seedcake additive amount is 15~30% (unsaturated fatty acid=1.15 n-3:n-6~1.18:1), effect is best.
In addition, as shown in Table 16, the mystus nemurus fed using 2 feed of example, plasma malonaldehyde content is substantially less than real 5 feed feeding group of example；Conversely, 2 feed feeding group Liver Superoxide Dismutase Activity of example, catalase and peroxidase activity Property is significantly higher than 5 feed feeding group of example.Antioxidant of the lycopene as fish mixed feed improves mystus nemurus antioxygen Change ability effect is substantially better than ethoxyquinoline.
Table 17, the analysis of each example 1-4 feed cost
As shown in Table 17: when rubber seedcake additive capacity is 15~45% in mystus nemurus mixed feed, per kilogram mystus nemurus Weight gain saves 0.41~0.60 yuan of feed cost.Using culture efficiency as reference frame, rubber seedcake in mystus nemurus mixed feed Additive capacity is 15~45% more appropriate.
In addition, as shown in Table 17, relative to 5 feed feeding group of example, the weight gain of 2 feed feeding group per kilogram mystus nemurus of example Save 0.27 yuan of feed cost.Using culture efficiency as reference frame, use lycopene as anti-in mystus nemurus mixed feed Oxidant is better than ethoxyquinoline.
In conclusion adding 15~30% rubber seedcakes in mystus nemurus mixed feed to mystus nemurus growth performance, feed benefit With rate, resistance to oxidation and immunity index without obvious adverse effect；And when additive amount is 15%, mystus nemurus growth performance is raised Material utilization rate, resistance to oxidation and immunity index are improved；But when additive amount reaches 45%, mystus nemurus growth performance is raised Material utilization rate and resistance to oxidation are decreased obviously.Therefore, when rubber seedcake additive amount is 15~30% in mystus nemurus mixed feed, it is Optimum proportioning, n-3:n-6 unsaturated fatty acid ratio is 1.15~1.18:1 in mixed feed at this time, both will not be raw to mystus nemurus Long performance and oxidation resistance generate adverse effect, and the optimization of aquatic products quality and culture efficiency may be implemented.
Finally, it is stated that preferred embodiment above is only used to illustrate the technical scheme of the present invention and not to limit it, although logical It crosses above preferred embodiment the present invention is described in detail, however, those skilled in the art should understand that, can be Various changes are made to it in form and in details, without departing from claims of the present invention limited range.

Claims (2)

1. a kind of efficient fish mixed feed with anti-oxidation function, it is characterised in that: replace beans using rubber seedcake part The dregs of rice or fish meal add lycopene as forage protein source in feed formula.
2. a kind of efficient fish mixed feed with anti-oxidation function according to claim 1, it is characterised in that: described The efficient fish mixed feed with anti-oxidation function include fish meal, rubber seedcake, methionine, lysine, leucine, fish Oil, soybean oil, soybean lecithin, lycopene, wheat-middlings, alphalise starch, vitamin C phosphoric ester, calcium dihydrogen phosphate, salt, chlorination Choline, vitamin premix, mineral substance premix, wherein the weight percent of various raw materials are as follows: fish meal 39.5~47.8%, rubber Glue seedcake 15~45%, methionine 0.07~0.22%, lysine 0.15~0.47%, leucine 0.07~0.22%, fish oil 0.1~ 0.6%, soybean oil 0.1~3.1%, soybean lecithin 0.5~1.0%, lycopene 0.01~0.03%, wheat-middlings 10.84~ 21.46%, alphalise starch 1~8%, vitamin C phosphoric ester 0.02~0.05%, calcium dihydrogen phosphate 0.7~1.2%, salt 0.2~ 0.3%, choline chloride 0.2~0.3%, vitamin premix 0.5~0.8%, mineral substance premix 0.8~1.0%；All raw materials The sum of weight percent is 100%.
CN201811118999.4A 2017-07-05 2017-07-05 A kind of efficient fish mixed feed with anti-oxidation function Pending CN109123236A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811118999.4A CN109123236A (en) 2017-07-05 2017-07-05 A kind of efficient fish mixed feed with anti-oxidation function

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201811118999.4A CN109123236A (en) 2017-07-05 2017-07-05 A kind of efficient fish mixed feed with anti-oxidation function
CN201710543223.6A CN107149057B (en) 2017-07-05 2017-07-05 A kind of efficient fish mixed feed with anti-oxidation function

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201710543223.6A Division CN107149057B (en) 2017-07-05 2017-07-05 A kind of efficient fish mixed feed with anti-oxidation function

Publications (1)

Publication Number Publication Date
CN109123236A true CN109123236A (en) 2019-01-04

Family

ID=59795140

Family Applications (3)

Application Number Title Priority Date Filing Date
CN201811118999.4A Pending CN109123236A (en) 2017-07-05 2017-07-05 A kind of efficient fish mixed feed with anti-oxidation function
CN201811118998.XA Pending CN109123235A (en) 2017-07-05 2017-07-05 A kind of efficient fish mixed feed with anti-oxidation function
CN201710543223.6A Active CN107149057B (en) 2017-07-05 2017-07-05 A kind of efficient fish mixed feed with anti-oxidation function

Family Applications After (2)

Application Number Title Priority Date Filing Date
CN201811118998.XA Pending CN109123235A (en) 2017-07-05 2017-07-05 A kind of efficient fish mixed feed with anti-oxidation function
CN201710543223.6A Active CN107149057B (en) 2017-07-05 2017-07-05 A kind of efficient fish mixed feed with anti-oxidation function

Country Status (1)

Country Link
CN (3) CN109123236A (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111567683A (en) * 2020-06-24 2020-08-25 广东海洋大学 Fish feed additive for improving antioxidant function
CN111743036A (en) * 2020-06-24 2020-10-09 广东海洋大学 Fish feed additive for improving antioxidant and immune functions
CN116784438B (en) * 2023-07-10 2023-12-26 佛山市顺德区旺海饲料实业有限公司 Feed for preventing soybean meal from inducing damage to endoplasmic reticulum of tilapia and preparation method of feed
CN117044832A (en) * 2023-08-25 2023-11-14 广州市联鲲生物科技有限公司 Additive, preparation method and application thereof in improving muscle quality of grass carp

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101305770A (en) * 2008-07-16 2008-11-19 东北农业大学 Feedstuff additive containing lycopene
CN101828641A (en) * 2010-04-07 2010-09-15 江苏微生物研究所有限责任公司 Animal feed using lycopene fungus oil as additive
CN103284018A (en) * 2013-06-28 2013-09-11 云南农业大学 Tilapia mossambica formula feed with rubber seedcakes and soya bean meal serving as main protein raw materials
US20150208696A1 (en) * 2012-08-03 2015-07-30 Kyowa Hakko Bio Co., Ltd. Feed for farmed fish and method for promoting growth of farmed fish using the same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103300256B (en) * 2013-06-28 2014-05-07 云南农业大学 Application of rubber seedcake to cyprinus carpio mixed feed and formula of feed
CN106721526B (en) * 2016-11-29 2019-08-02 河南科技大学 A kind of preparation method of the Yellow River carp anti-stress feed

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101305770A (en) * 2008-07-16 2008-11-19 东北农业大学 Feedstuff additive containing lycopene
CN101828641A (en) * 2010-04-07 2010-09-15 江苏微生物研究所有限责任公司 Animal feed using lycopene fungus oil as additive
US20150208696A1 (en) * 2012-08-03 2015-07-30 Kyowa Hakko Bio Co., Ltd. Feed for farmed fish and method for promoting growth of farmed fish using the same
CN103284018A (en) * 2013-06-28 2013-09-11 云南农业大学 Tilapia mossambica formula feed with rubber seedcakes and soya bean meal serving as main protein raw materials

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
温辉梁主编: "《饲料添加剂生产技术与配方》", 31 December 2009, 南昌：江西科学技术出版社 *

Also Published As

Publication number Publication date
CN107149057A (en) 2017-09-12
CN109123235A (en) 2019-01-04
CN107149057B (en) 2019-01-04

Similar Documents

Publication Publication Date Title
CN107149057B (en) A kind of efficient fish mixed feed with anti-oxidation function
CN106879864B (en) Yellow-feather broiler feed capable of improving meat quality efficiently and at low cost and application of yellow-feather broiler feed
CN103340308B (en) Application of fibrauretine in cyprinus carpio compound feed and formula of cyprinus carpio compound feed containing fibrauretine
CN103349156B (en) Oligopeptide-rich powder-type compound feed for anguilla marmorata
CN109198214A (en) A kind of fermented feed, preparation method and its mixed feed
Gamboa-Delgado et al. Comparing the assimilation of dietary nitrogen supplied by animal-, plant-and microbial-derived ingredients in Pacific white shrimp Litopenaeus vannamei: A stable isotope study
CN104719679B (en) Application and its formula of a kind of rubber seed oil in rainbow trout mixed feed
CN103262959B (en) Puffing settleable mixed feed for adult fish of fugu obscurus
CN103300222B (en) Application of rubber seedcake to oncorhynchus mykiss mixed feed and formula of feed
CN111938044A (en) Nano-selenium feed for reducing excessive fat accumulation in abdominal cavity of grass carp
CN115462473B (en) Daily ration for improving eggshell quality in later period of egg production and preparation method thereof
Gaylord et al. Re‐evaluation of vitamin E supplementation of practical diets for channel catfish, Ictalurus punctatus, production
CN103300256B (en) Application of rubber seedcake to cyprinus carpio mixed feed and formula of feed
CN103300223B (en) Application of rubber seedcake in megalobrama amblycephala formula feed and formula of megalobrama amblycephala formula feed
CN103300255B (en) Application of rubber seedcake in tilapia formula feed and formula of tilapia formula feed
CN105767546B (en) A kind of piglet premixed feed and mixed feed
Andriani et al. Utilization of fermented Mangrove propagules (Rhizophora mucronata) as feeding material for Nile tilapia (Oreochromis niloticus)
CN107259216B (en) A kind of fish feed ready-mixed oil with anti-oxidation function
CN104719678B (en) Application and its formula of a kind of rubber seed oil in compound feed for carps
WO2021015648A2 (en) Protein feed additive for livestock animals and fish
Gabr et al. UTILIZATION OF DISTILLERS DRIED GRAINS WITH SOLUBLES IN FISH NUTRITION 2-PARTIAL REPLACEMENT OF FISH MEAL AND YELLOW CORN BY GRADED LEVELS OF DDGS IN NILE TILAPIA FINGERLINGS DIETS (Oreochromis niloticus).
CN104719637B (en) Application and its formula of a kind of rubber seed oil in sturgeon mixed feed
CN1281733C (en) Yangqi penicillium capable of giving high yield of alpha-alactosidase as well as its application for preparing feeding alpha-alactosidase in solid state fermentation
CN104719677B (en) A kind of sturgeon mixed feed that rubber seedcake utilization rate is improved using yolk powder
CN111789197A (en) Feed for laying hens laying yellow mealworms for first time

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20190104